Spectrophotometry

(Infrared Spectrophotometry)

dela Cruz, Charmaine L.

B.S. Biology 3-1d

Prof. Rafael Eleazar

Spectrophotometry
• A spectrophotometer is employed to
measure the amount of light that a
sample absorbs. The instrument operates
by passing a beam of light through a
sample and measuring the intensity of
light reaching a detector.
•
Infrared Spectrophotometry
• Infrared Spectrophotometry is designed to
identify or determine the sample by measuring
absorption of infrared radiation when it passes
through the sample



Typical Apparatus used in
IR Spectrophotometry
The infrared portion of the
electromagnetic
spectrum is usually divided into three

regions:


• the near-infrared
•
• the mid-infrared; and


• the far- infrared


Biological Applications


• Determination of enzymatic
activity
• Diagnosis of certain diseases
