Brianna Zajac

3rd hour
Testing Organic Molecules
Background Information
The macromolecules in this experiment were distilled water, monosaccharide,
polysaccharide, lipid, and protein. The indicators in this experiment were benedicts
solution, which needs to be heated in a warm water bath, biurets solution, iodine,
and a brown paper bag. First, when Benedicts solution was tested on all of the
macromolecules, the only macromolecule that changed was the monosaccharide.
The Benedicts solution changed from a light Caribbean blue color to an orange
color. Second, when Biurets solution was tested on all of the macromolecules, the
only macromolecule that changed was protein. The Biurets solution changed from
a light blue color to a light purple color. Next, when Iodine was tested on all of the
macromolecules, the only one that changed was the polysaccharide. The iodine
changed from a dark rusty color to immediately black. Finally when the brown
paper bag was tested on all the macromolecule, the only one that changed was the
lipid. The lipid on the paper bag looked wet even though it was dry.
Statement of the Problem
The problem in this experiment is trying to find out if the new energy
supplement bar number two/B contains all protein and no carbohydrates or fats.
The Food and Drug Administration (FDA) is in charge of testing the validity of the
company marketing claims.
Hypothesis

If a meal supplement bar contains all protein and no carbohydrates or fats,

then it is valid company marketing claim.
Materials

Small hot water bath

Test tubes
Test tube holders
Beakers
Eye droppers
Plastic palette
Brown paper bag
Monosaccharide
Polysaccharide
Lipid
Protein
Distilled water
Benedicts solution
Unknown sample B
Biurets solution
Iodine
Chart for information
Pencil
Timer
Sink
Tongs
White piece of paper
soap

Procedure
1. Gather all the materials
2. Turn on the hot water bath
3. Label the palette according to where the macromolecules and the indicators
are going to go (rows and columns)
4. Put the palette on a white piece of paper
5. Assign each indicator, except the brown paper bag, a beaker
6. Assign each indicator except for the brown paper bag and each
macromolecule a specific eyedropper
7. Put five to ten drops of each macromolecule using the specific eyedroppers in
the specified spots (there should be three spots for each macromolecule)

8. Record what each macromolecule looks like before any indicators change the
characteristics
9. Put each indicator in one spot on the palette per indicator using the assigned
eyedroppers
10.Record what each indicator looks like in the chart
11.First test the Biurets solutions by putting five to ten drops using the biurets
solution eyedropper in one spot per macromolecule.
12.Wait three to five minutes and record the results in the chart
13.Next, test the iodine by putting five to ten drops using the iodine eyedropper
in one spot per macromolecule
14.Wait three to five minutes and record the results in the chart
15.Put the test tubes in the test tube holder
16.Assign each macromolecule a specific test tube
17.Put five to ten drops of the monosaccharide by using the monosaccharide
eyedropper in the monosaccharide test tube.
18.Put five to ten drops of the Benedicts solution in the monosaccharide test
tube by using the benedicts solution eyedropper
19.Carry the test tube with the tong to the hot water bath and hold it in there for
three to five minutes.
20.Carry it back to the test tube holder by using the tongs.
21.Record results in the chart
22.Dump the results out in the sink and wash the test tube with soap and warm
water
23.Put the test tube in the test tube holder
24.Put five to ten drops of the polysaccharide by using the polysaccharide
eyedropper in the polysaccharide test tube.
25.Put five to ten drops of the Benedicts solution in the polysaccharide test tube
by using the benedicts solution eyedropper
26.Repeat steps 17-21
27.Put five to ten drops of the lipid by using the lipid eyedropper in the lipid test
tube
28.Put five to ten drops of the Benedicts solution in the lipid test tube by using
the benedicts solution eyedropper
29.Repeat steps 17-21
30.Put five to ten drops of the protein by using the protein eyedropper in the
protein test tube

31.Put five to ten drops of the Benedicts solution in the protein test tube by
using the benedicts solution eyedropper
32.Repeat steps 17-21
33.Divide the brown paper bag in four equal sections
34.Assign each macromolecule a specific section
35.Put one to two drops of each macromolecule in the specific section
36.Wait five minutes and record the results in the chart
37.Throw away paper towel
38.Wash the palette with soap and warm water in the sink
39.Assign the unknown sample an eyedropper
40.Put the palette on a white piece of paper
41.Put five to ten drops of the unknown sample in three spots on the palette
42.Assign the biurets solution and iodine a spot on the palette
43.Record what the unknown sample looks like without an indicator in the chart
44.Put five to ten drops of the biurets solution in its specified spot
45.Wait three to five minutes and record results in the chart
46.Put five to ten drops of the iodine in its specified spot
47.Wait three to five minutes and record results in the chart
48.Get a new paper bag and put one to two drops of the unknown sample on the
paper bag
49.Wait five minutes and record the results on the chart
50.Assign the unknown sample a test tube.
51.Put five to ten drops of the unknown sample in a test tube using the unknown
samples eyedropper in the unknown samples test tube.
52.Put five to ten drops of the Benedicts solution in the unknown sample test
tube by using the benedicts solution eyedropper
53.Repeat steps 17-21
54.Wash the palette with soap and warm water in the sink and put it away
55.Throw away the paper towel
56.Put the test tubes and the test tube holder away
57.Wash all the eyedroppers with warm water and soap in the sink and put them
away
58.Put all the caps on the macromolecules and put them away
59.Put the tongs, white piece of paper, and the timer away
60.Dump the extra indicators down the sink and wash the beakers with soap and
warm water in the sink
61.Put the beakers away
62.Turn off the hot water bath and dump the water in the sink
Conclusions

I reject my hypothesis for many reasons. First I reject it because the

unknown sample contained not just protein but also monosaccharaides and fats.
How I figured this out was when we tested the benedicts solution on all of the
macromolecules, the only macromolecule that changed was the
monosaccharide. The benedicts solution changed from a light Caribbean blue to
an orange color. When we tested the biurets solution on all of the
macromolecules, the only macromolecule that changed was the protein. The
biurets solution changed from a light blue color to a light purple color. When we
tested the iodine on all of the macromolecules, the only macromolecule that
changed was the polysaccharide. The iodine changed from a rusty brown color
to immediately black. When we tested the brown paper bag on all of the
macromolecules, the only macromolecule that changed was the lipid. The lipid
dried but it still looked wet and was transparent. When we tested the unknown
sample on all of the indicators, the benedicts solution changed to an orange
color, the biurets solution changed to a light purple, the iodine didnt change,
and the unknown sample dried but still looked wet. This indicated that the
unknown sample contained carbohydrates, fats, and proteins. One thing that I
learned from this experiment is that you cant trust everything that businesses
claim. Some sources of error could be cross-contamination of the eyedropper.
Also there could have been some other samples in the palette or the test tubes
that could throw off the results. Lastly, there could be confusion in which
substance is which. This could occur by not labeling the substances clearly or
properly. This is why I rejected my hypothesis.