Description

ProductSheet

Bacilluspumilus(ATCC

Designation:NCTC8241[CCM2218,DSM361,NCIB8982]
DepositedName:BacilluspumilusMeyerandGottheil
ProductDescription:Usedintheassayofneomycin,framycetin,gentamicin,lymecycline,erythromycin,
tetracycline,oxytetracycline[terramycin],andchlortetracycline[aureomycin].

Propagation

14884)

Medium
ATCCMedium3:Nutrientagarornutrientbroth

PleasereadthisFIRST

StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

GrowthConditions
Temperature:30C
Atmosphere:Aerobic
PropagationProcedure
1. Openvialaccordingtoenclosedinstructions.
2. Usingasingletubeof#3broth(5to6mL),withdrawapproximately0.5to1.0mLwithaPasteuror
1.0mLpipette.Rehydratetheentirepellet.
3. Asepticallytransferthisaliquotbackintothebrothtube.Mixwell.
4. Useseveraldropsofthesuspensiontoinoculatea#3agarslantand/orplate.
5. Incubatethetubesandplateat30Cfor24to48hours.

Notes
Colonieson#3appearcircular,irregular,opaque,flat,glistening,andrough.
Colonialvariationmaybeobservedandistypicalforthisstrain.
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Bacilluspumilus(ATCC14884)

References
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.

BiosafetyLevel:1
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty
TheviabilityofATCCproductsiswarrantedfor30daysfromthedateofshipment,andisvalidonlyifthe
productisstoredandculturedaccordingtotheinformationincludedonthisproductinformationsheet.ATCC
liststhemediaformulationthathasbeenfoundtobeeffectiveforthisstrain.Whileother,unspecifiedmedia
mayalsoproducesatisfactoryresults,achangeinmediaortheabsenceofanadditivefromtheATCC
recommendedmediamayaffectrecovery,growthand/orfunctionofthisstrain.Ifanalternativemedium
formulationisused,theATCCwarrantyforviabilityisnolongervalid.

Disclaimers

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page1of2

Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproduct
sheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliterature
andpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhas
beenconfirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.
ATCCisnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.While
reasonableeffortismadetoensureauthenticityandreliabilityofstrainsondeposit,ATCCisnotliablefor
damagesarisingfromthemisidentificationormisrepresentationofcultures.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2014.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[03/14]

ProductSheet

Bacilluspumilus(ATCC
14884)
PleasereadthisFIRST

StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Bacilluspumilus(ATCC14884)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page2of2