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THE EFFECT OF UV-B RAY MUTAGEN ON MICROALGAE

BOTRYOCOCCUS BRAUNII AND NANNOCHLOROPSIS SP. GROWTH AND


LIPID PRODUCTIVITY

Name /NRP
:
1.Dinny Amrina Maharani
NRP.2312 100 066
2.Muhammad Helmi Faisal
NRP.2312 100 118
Advisor
: Prof.Dr.Ir.Arief Widjaja,M.Eng

ABSTRACT

The current technology leads to the alternative energy sources as replacement


for non-renewable energy. One of them is the of biodiesel manufacture as a subtitution
of diesel fuel. Microalgae are a viable source of renewable energy to be developed.
Botryococcus braunii and Nannochloropsis sp. are microalgae that currently being
tested for its ability as diesel fuel source because of its high lipid content. Mutagenesis
were done to improve the productivity of lipids, which used UV B rays as mutagen.
The first stage was to do microalgae Botryococcus braunii and Nannochloropsis
sp. cultivation for 14 days and nutrients adding once every 12 hours. The second stage
was to determine the effect of UV-B ray mutagen on the growth of microalgae in
different medium pH. The third stage was lipid extraction. Lipid extraction process
steps include of centrifugation, drying in an oven at 60 C for 2 hours, soxhlet
extraction using n-hexane solvent, separation of lipids from the solvent with a rotary
vacuum evaporator. Furthermore GCMS analysis was used to determine the content of
the compound of microalgae oil, DNA sequencing to determine the sequence of DNA
bases her.
Key words: Microalgae, Botryococcus braunii, Nannochloropsis sp, mutation, UV B

iv

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