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GXM & GSH Antiglobulin test (Coombs test)
AHG (Antihuman globulin) – produced via animals by injection of human globulin,
Compatibility testing compleme nt, immunoglob ulin
Prevent haemolysis AHG + Human RBC (coated with Ig antibody) or complement
Agglutination – indicate +ve test
Donor’s blood is not compatible/ safe for transfusion
Blood grouping
AHG enhance red cell agglutination (red cells coated by antibodies)
Reagents/ equipments
Anti A, B, AB (direct grouping)
Blood from recipient (cells, serum)
Tile, Test tubes
Centrifuge
Known cells – O, A, B (indirect grouping)
Principle
Antigen-antibody reaction causes RBC agglutination