Degree of cell injury of human erythrocytes due to storage in to

different preservative solutions

Complex structures, like the human erythrocytes, require an in-depth and

deeper analysis than mere simple dielectrics. Storage conditions further enhance

the complexities of cellular biological systems. A composite system containing

polarisable and conductive materials may be used to deal with such systems.

When the erythrocytes are plunged into preservative solutions, they get shocked

thermally, chemically & somatically. As a result, their steady state will be

disturbed resulting in the changes in their physiology. The storage period also

affects significantly on the cellular physiology of erythrocytes.

The Dielectrophoretic spectrum is unique & characteristic of cell

physiology. The subtle changes in the cell physiology & biochemistry can be

sensed in a better way by using the technique of biological cell DEP. The

biological cell DEP can be used as an important tool to understand the drastic

changes in the 4-layered structure of human erythrocytes membrane.

The present study focuses on the degree of cell injury or percent change in

different electrical parameters in particular the DCR of human erythrocytes of 4

major human blood groups A, B, AB and O. The degree of cell injury of human

erythrocytes, it is evident that after 8 days of refrigerated storage in EDTA, ACD

& CPD-A, blood used for transfusion is not safe and may create different

problems to the normal health of human beings and after two weeks of

refrigerated storage, the material used for transfusion can merely be described as

blood.