Stock Acetosyringone Transcultivation Media Plates
98.1 mg/1 mL of 95% EtOH = 500 mM 4.4 g MS 25 g Sucrose MS Plates 2.5 g Gelrite/Gelzan 4.4 g MS pH 5.7 – 5.9 25 g Sucrose Autoclave 15 min. 2.5 g Gelrite/Gelzan After cooling – Add in pH 5.7 – 5.9 250 µL NAA, Autoclave 15 min. 2000 µL Zeatine, SIM Media 1000 µL Cefotaxime, 5.882 g Sodium Citrate 2000 µL Kanamycin, 20 g Sucrose 1000 µL Vancomycin pH 5.5 Root Media Plates Autoclave 15 min. 4.4 g MS After cooling - Add in 25 g Sucrose X µL Acetosyringone, 2.5 g Gelrite/Gelzan X µL Spectinomycin, pH 5.7 -5.9 X µL Chloramphenicol Autoclave 15 min. for every X mL bacteria culture After cooling – Add in Co-Cultivation Media (Liquid) 200 µL NAA, 4.4 g MS 1000 µL Cefotaxime, 25 g Sucrose 2000 µL Kanamycin, pH 5.7 – 5.9 1000 µL Vancomycin Autoclave 15 min.
Co-Cultivation Media (Solid)
4.4 g MS 25 g Sucrose 2.5 g Gelrite/Gelzan pH 5.7 – 5.9 Autoclave 15 min. After cooling – Add in 2000 µL NAA, 2000 µL Zeatine, 250 µL Acetosyringone