Alleleustrious pmTFP1-ER

Source Catalog Number: Introduction All ustrious pmTFP1-ER is a mammalian expression vector that includes a Calreticulin (CALR) signal sequence to the N-terminus of mTFP1 and ER retention signal sequence KDEL to the C-terminus. This product can be used as a subcellular marker for the endoplasmic reticulum (ER). The properties of All ustrious mTFP1 are superior to those of the commonly used Aequorea victoria variant ECFP and which can be easily detected in the mitotic spindle of mammalian cells using standard CFP filter sets. In addition, the fluorescent signal from mTFP1 is significantly brighter than that from EGFP using standard GFP filter sets. mTFP1 also makes an excellent fluorescence resonance energy transfer (FRET) donor to yellow or orange fluorescent proteins. Calreticulin is a Ca2+-binding chaperone found in the lumen of the endoplasmic reticulum (ER). It binds monoglucosylated oligosaccharides and misfolded proteins in a quality control processes during protein synthesis and folding. It is also a factor in regulating Ca2+ homeostasis. This fusion protein retains the calreticulin signal on the N-terminus and the KDEL (lys-asp-glu-leu) on the C-terminus, which should direct mTFP1 into ER.
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ABP-FP-TER

Engineered variant of Clavularia sp. fluorescent protein cFP484. Recommended Use To further optimize detection, the use of filter sets specific to the mTFP1 excitation and emission spectra is recommended. Features More than 3-fold brighter than ECFP More than twice as photostable as ECFP Single emission peak Single-exponential fluorescence lifetime ( = 3.2 ns) Can be co-imaged with red and yellow FPs Low sensitivity to acidic pH (fluorescence pKa=4.3) Mammalian expression vector ready to transfect your favorite cells True monomer that will not aggregate or cause non-specific interactions Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 Calreticulin signal sequence....597-656 mTFP1 657-1364 KDEL ER retention signal..1365-1379 SV40 PolyA Signal.....................1570-1620 bla Promoter...............................2159-2263 Ampicillin Resistance Gene......2248-3108 pUC Origin..................................3257-3899 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA AMPR pUC Ori PCMV Calreticulin Signal Sequence

pmTFP1-ER 3981bp

mTFP1

KDEL ER Retention Signal SV40 Poly A

References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Nakamura K, Zuppini A, Arnaudeau S, Lynch J, Ahsan I, Krause R, Papp S, De Smedt H, Parys JB, Muller-Esterl W, Lew DP, Krause KH, Demaurex N, Opas M, Michalak M. "Functional specialization of calreticulin domains." J Cell Biol. 2001 Sep 3;154(5):961-72. Rokeach,L.A., Haselby,J.A., Meilof,J.F., Smeenk,R.J., Unnasch,T.R., Greene,B.M. and Hoch,S.O.. "Characterization of the autoantigen calreticulin" J. Immunol. 1991 147 (9), 3031-3039

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-ER
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG TGCTATCCGT TGAGCAAGGG AGATGGAGGG CCTACGACGG CCTACGACAT ACGACATCCC TGACCTTCGA CCTTCATCTA AGAAGAAGAC TGAAGGGCGA TCAAGACCAT ACCGCATCGA CCGTGGCCCG AATTCTGCAG ATCAGCCATA CTGAACCTGA AATGGTTACA CATTCTAGTT ATTAAGCGCG AGCGCCCGCT TCAAGCTCTA CCCCAAAAAA TTTTCGCCCT AACAACACTC GGCCTATTGG ATTAACGCTT TTATTTTTCT CTTCAATAAT CCCTTTTTTG AAAGATGCTG GGTAAGATCC GTTCTGCTAT CGCATACACT ACGGATGGCA GCGGCCAACT AACATGGGGG CCAAACGACG TTAACTGGCG GATAAAGTTG AAATCTGGAG AAGCCCTCCC AATAGACAGA GTTTACTCAT GTGAAGATCC TGAGCGTCAG GTAATCTGCT CAAGAGCTAC ACTGTTCTTC ACATACCTCG CTTACCGGGT GGGGGTTCGT CAGCGTGAGC GTAAGCGGCA TATCTTTATA TCGTCAGGGG GCCTTTTGCT AACCGTATTA TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA GCCGTTGCTG CGAGGAGACC CAACGTGAAT CACCAACACC TCTGACCACC CAACTACTTC GGACAAGGGC CGAGATACAC CACCGGCTGG CGTCAAGCAC CTACAGGGCC GATCCTGAAC CAACTCCACC TCGACGGTAC CCACATTTGT AACATAAAAT AATAAAGCAA GTGGTTTGTC GCGGGTGTGG CCTTTCGCTT AATCGGGGGC CTTGATTAGG TTGACGTTGG AACCCTATCT TTAAAAAATG ACAATTTAGG AAATACATTC ATTGAAAAAG CGGCATTTTG AAGATCAGTT TTGAGAGTTT GTGGCGCGGT ATTCTCAGAA TGACAGTAAG TACTTCTGAC ATCATGTAAC AGCGTGACAC AACTACTTAC CAGGACCACT CCGGTGAGCG GTATCGTAGT TCGCTGAGAT ATATACTTTA TTTTTGATAA ACCCCGTAGA GCTTGCAAAC CAACTCTTTT TAGTGTAGCC CTCTGCTAAT TGGACTCAAG GCACACAGCC TATGAGAAAG GGGTCGGAAC GTCCTGTCGG GGCGGAGCCT GGCCTTTTGC CCGCCATGCA TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CTCGGCCTCC ACAATGGGCG GGCCACGCCT ATCAACCTGG GCGTTCGCCT AAGCAGTCCT ATCGTGAAGG CTCAAGGGCG GACGCCTCCA AAGCTGCTGC AAGAAGGCGG CACGACAAGG GACGGCATGG CGCGGGCCCG AGAGGTTTTA GAATGCAATT TAGCATCACA CAAACTCATC TGGTTACGCG TCTTCCCTTC TCCCTTTAGG GTGATGGTTC AGTCCACGTT CGGTCTATTC AGCTGATTTA TGGCACTTTT AAATATGTAT GAAGAGTATG CCTTCCTGTT GGGTGCACGA TCGCCCCGAA ATTATCCCGT TGACTTGGTT AGAATTATGC AACGATCGGA TCGCCTTGAT CACGATGCCT TCTAGCTTCC TCTGCGCTCG TGGGTCTCGC TATCTACACG AGGTGCCTCA GATTGATTTA TCTCATGACC AAAGATCAAA AAAAAAACCA TCCGAAGGTA GTAGTTAGGC CCTGTTACCA ACGATAGTTA CAGCTTGGAG CGCCACGCTT AGGAGAGCGC GTTTCGCCAC ATGGAAAAAC TCACATGTTC T ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC TCGGCCTGGC TAATCAAGCC TCGTGATCGA AGGTGAAGGA ACGGCAACAG TCCCCGAGGG TGAAGTCCGA AGAACTTCCC CCGAGAGGAT TGGAGGGCGG TGAAGCTGCC ACTACAACAA ACGAGCTGTA GGATCCACCG CTTGCTTTAA GTTGTTGTTA AATTTCACAA AATGTATCTT CAGCGTGACC CTTTCTCGCC GTTCCGATTT ACGTAGTGGG CTTTAATAGT TTTTGATTTA ACAAAAATTT CGGGGAAATG CCGCTCATGA AGTATTCAAC TTTGCTCACC GTGGGTTACA GAACGTTTTC ATTGACGCCG GAGTACTCAC AGTGCTGCCA GGACCGAAGG CGTTGGGAAC GTAGCAATGG CGGCAACAAT GCCCTTCCGG GGTATCATTG ACGGGGAGTC CTGATTAAGC AAACTTCATT AAAATCCCTT GGATCTTCTT CCGCTACCAG ACTGGCTTCA CACCACTTCA GTGGCTGCTG CCGGATAAGG CGAACGACCT CCCGAAGGGA ACGAGGGAGC CTCTGACTTG GCCAGCAACG TTTCCTGCGT AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC CGTCGCCGAC CGACATGAAG GGGCGAGGGC GGGAGCCCCC GGCCTTCACC CTACTCTTGG CATCTCCATG CCCCAACGGC GTACGTGCGC CGGCCACCAC CGACTATCAC GGTGACCGTT CAAGAAGGAC GATCTAGATA AAAACCTCCC ACTTGTTTAT ATAAAGCATT AACGCGTCCT GCTACACTTG ACGTTCGCCG AGTGCTTTAC CCATCGCCCT GGACTCTTGT TAAGGGATTT AACGCGAATT TGCGCGGAAC GACAATAACC ATTTCCGTGT CAGAAACGCT TCGAACTGGA CAATGATGAG GGCAAGAGCA CAGTCACAGA TAACCATGAG AGCTAACCGC CGGAGCTGAA CAACAACGTT TAATAGACTG CTGGCTGGTT CAGCACTGGG AGGCAACTAT ATTGGTAACT TTTAATTTAA AACGTGAGTT GAGATCCTTT CGGTGGTTTG GCAGAGCGCA AGAACTCTGT CCAGTGGCGA CGCAGCGGTC ACACCGAACT GAAAGGCGGA TTCCAGGGGG AGCGTCGATT CGGCCTTTTT TATCCCCTGA TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCATGC CGGTCGATGG ATCAAGCTGA GAGGGCAAGC CTGCCCTTCT AAGTACCCCG GAGCGCACCA GAGGAGGACT CCCGTGATGC GACGGCGTGC CGCGTTGACT TTTGTGGACC TACGAGAGCG GAGCTGTAAG ACTGATCATA ACACCTCCCC TGCAGCTTAT TTTTTCACTG GTAGCGGCGC CCAGCGCCCT GCTTTCCCCG GGCACCTCGA GATAGACGGT TCCAAACTGG TGCCGATTTC TTAACAAAAT CCCTATTTGT CTGATAAATG CGCCCTTATT GGTGAAAGTA TCTCAACAGC CACTTTTAAA ACTCGGTCGC AAAGCATCTT TGATAACACT TTTTTTGCAC TGAAGCCATA GCGCAAACTA GATGGAGGCG TATTGCTGAT GCCAGATGGT GGATGAACGA GTCAGACCAA AAGGATCTAG TTCGTTCCAC TTTTCTGCGC TTTGCCGGAT GATACCAAAT AGCACCGCCT TAAGTCGTGT GGGCTGAACG GAGATACCTA CAGGTATCCG AAACGCCTGG TTTGTGATGC ACGGTTCCTG TTCTGTGGAT

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-ER
Enzyme AatII Acc113I Acc16I Acc65I AccB1I AccB7I AccBSI AccI AcsI AcyI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII BalI BamHI BanI BanII BbiII BbsI BbuI Bbv12I Bbv16II BcgI BclI BcoI BglI BpiI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse8I BseRI Bsh1285I Bsh1365I BshNI BsiEI BsiHKAI BsiI BsmI BsoBI Bsp120I Bsp1407I Bsp143II Bsp19I BspCI BspHI BspLU11I BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssSI BssT1I BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I BstXI BstYI Cfr10I Cfr42I Cfr9I CfrI Cuts 5 1 1 1 5 1 2 1 4 7 2 1 1 3 2 1 2 1 2 4 2 2 1 1 1 3 2 1 1 1 5 3 7 1 1 3 1 1 1 2 6 1 3 1 3 1 7 2 2 4 5 4 1 3 4 1 5 4 3 2 2 2 1 1 4 2 1 2 1 2 1 2 4 1 4 2 2 2 4 1 4 1 4 1 6 1 10 1 10 4 1 1 4 Positions 125 178 261 447 1153 2554 2812 1396 465 789 1396 1851 3082 1096 1751 2196 1391 1380 1590 2075 2086 122 175 258 444 1102 1150 2495 1662 3923 649 3035 2367 2452 3613 2363 3609 3514 933 1407 1408 2363 3609 1380 1590 2075 2086 7 2860 7 2860 2435 1396 3035 2367 2452 3613 933 1407 2812 742 1411 465 789 1396 1851 3082 827 1408 1821 122 175 258 444 1102 1150 2495 1090 599 2367 2452 3613 1090 2519 1433 933 1407 90 212 283 631 1224 2917 1090 812 1175 2950 1090 340 1124 1892 1438 122 175 258 444 1102 1150 2495 680 2968 1526 1625 650 2517 2666 3589 649 1417 2739 3570 3717 649 1092 1787 2950 1438 632 677 1016 650 2517 2666 3589 1438 465 789 1396 1851 3082 650 2517 2666 3589 2367 2452 3613 2371 3755 1526 1625 933 1407 1404 1357 1321 1737 1745 3683 360 1006 2666 2195 3203 3923 1751 2196 1438 2799 2981 649 1092 1787 2950 1357 649 1092 1787 2950 2371 3755 360 1006 1751 2196 360 1006 1321 1400 1301 1321 1737 1745 3683 1411 650 2517 2666 3589 1301 1211 1385 1669 2789 3467 3658 340 585 1270 1411 1420 2388 2405 3173 3185 3271 3282 806 585 1270 1411 1420 2388 2405 3173 3185 3271 3282 649 1092 1787 2950 1403 1407 637 740 1181 2642 Sequence gacgt/c agt/act tgc/gca g/gtacc g/gyrcc ccannnn/ntgg gagcgg gt/mkac r/aatty gr/cgyc a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg gggcc/c g/tgcac r/aatty at/taat at/taat gaann/nnttc g/gtacc gacnnn/nngtc gwgcw/c c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc grgcy/c gr/cgyc gaagac gcatg/c gwgcw/c gaagac cgannnnnntgc t/gatca c/ycgrg gccnnnn/nggc gaagac ctggag gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy gatnn/nnatc gaggag cgry/cg gatnn/nnatc g/gyrcc cgry/cg gwgcw/c ctcgtg gaatgc c/ycgrg g/ggccc t/gtaca rgcgc/y c/catgg cgat/cg t/catga a/catgt gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy ccannnnn/ntgg r/gatcy r/ccggy ccgc/gg c/ccggg y/ggccr DraI DraII DraIII DrdI DsaI EaeI Eam1104I Eam1105I EarI EclHKI Eco105I Eco130I Eco24I Eco255I Eco31I Eco57I Eco64I Eco88I Eco91I EcoO109I EcoO65I EcoRI EcoT14I EcoT22I ErhI Esp1396I FauNDI FbaI FriOI FspI GsuI HaeII Hin1I HincII HindII HpaI Hsp92I KpnI Ksp22I Ksp632I KspI MamI MfeI MflI MluI MluNI Mph1103I MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NcoI NdeI NgoAIV NgoMI NheI NsiI NspBII NspI PaeI PflMI PinAI Ple19I Ppu10I PshBI Psp1406I PspAI PspALI PspEI PspOMI PstI PstNHI PvuI RcaI SacII SalI ScaI SfcI SfiI Sfr303I SmaI SnaBI SphI SspBI SspI SstII StyI Van91I VneI VspI XbaI 4 1 2 2 4 4 1 1 1 1 1 2 3 1 2 4 5 2 1 1 1 1 2 1 2 1 1 1 3 1 3 4 7 3 3 1 7 1 1 1 1 1 1 10 1 1 1 1 1 5 7 4 1 2 1 2 1 1 1 1 1 4 2 1 1 1 1 1 2 2 1 1 1 1 1 1 1 2 1 1 1 6 1 1 1 1 1 1 2 1 2 1 2 2 1 1478 2457 3149 3168 880 1253 1895 1939 3821 360 1006 1321 1400 637 740 1181 2642 2246 3035 2246 3035 340 360 1006 827 1408 1821 2554 680 2968 722 1145 2353 3401 465 789 1396 1851 3082 933 1407 1301 880 1301 1380 360 1006 3980 360 1006 1096 234 1433 827 1408 1821 2812 812 1175 2950 1321 1737 1745 3683 122 175 258 444 1102 1150 2495 1196 1392 1539 1196 1392 1539 1539 122 175 258 444 1102 1150 2495 1400 1433 2246 1403 1438 1526 585 1270 1411 1420 2388 2405 3173 3185 3271 3282 1662 742 3980 1787 742 365 1005 2264 2623 2782 122 175 258 444 1102 1150 2495 1402 2399 3340 3585 1526 1526 1625 1789 360 1006 234 1787 1787 591 3980 1402 2399 3340 3585 599 3927 599 1096 649 2666 3976 7 2860 2433 2806 1407 1409 1301 1404 1389 591 2666 2195 3203 1403 1390 2554 1211 1385 1669 2789 3467 3658 631 1403 1409 340 599 1357 2100 2230 1403 360 1006 1096 2363 3609 7 2860 1423 ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr ctcttc gacnnn/nngtc ctcttc gacnnn/nngtc tac/gta c/cwwgg grgcy/c agt/act ggtctc ctgaag g/gyrcc c/ycgrg g/gtnacc rg/gnccy g/gtnacc g/aattc c/cwwgg atgca/t c/cwwgg ccannnn/ntgg ca/tatg t/gatca grgcy/c tgc/gca ctggag rgcgc/y gr/cgyc gty/rac gty/rac gtt/aac gr/cgyc ggtac/c t/gatca ctcttc ccgc/gg gatnn/nnatc c/aattg r/gatcy a/cgcgt tgg/cca atgca/t g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc atgca/t cmg/ckg rcatg/y gcatg/c ccannnn/ntgg a/ccggt cgat/cg a/tgcat at/taat aa/cgtt c/ccggg ccc/ggg g/gtnacc g/ggccc ctgca/g g/ctagc cgat/cg t/catga ccgc/gg g/tcgac agt/act c/tryag ggccnnnn/nggcc ccgc/gg ccc/ggg tac/gta gcatg/c t/gtaca aat/att ccgc/gg c/cwwgg ccannnn/ntgg g/tgcac at/taat t/ctaga

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-ER
XcmI 1 ccannnnn/nnnntgg XhoII 10 1343 585 1270 1411 1420 2388 2405 r/gatcy XmaI XmnI Zsp2I 1 1 1 3173 3185 3271 3282 1407 2435 3980 c/ccggg gaann/nnttc atgca/t

The following enzymes do not cut: AatI, AccIII, AclNI, AfeI, AflII, AocI, Aor51HI, AscI, AspI, AtsI, AvrII, BanIII, BbeI, BbrPI, BfrI, BglII, BlnI, BlpI, Bpu1102I, Bpu14I, Bsa29I, BscI, Bse21I, BseAI, BseCI, BsePI, BsgI, BsiMI, BsiWI, BsmBI, Bsp106I, Bsp119I, Bsp13I, Bsp1720I, Bsp68I, BspDI, BspEI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, BstBI, BstZI, Bsu15I, Bsu36I, CciNI, CelII, ClaI, CpoI, Csp45I, CspI, CvnI, EagI, Ecl136II, EclXI, Eco147I, Eco32I, Eco47III, Eco52I, Eco72I, Eco81I, EcoICRI, EcoNI, EcoRV, EheI, Esp3I, FseI, HindIII, KasI, Kpn2I, LspI, MroI, MspCI, NarI, NotI, NruI, NspV, PacI, PaeR7I, Pfl23II, PmaCI, Pme55I, PmeI, PmlI, PpuMI, PshAI, Psp124BI, Psp5II, PspLI, PvuII, RsrII, SacI, SapI, SbfI, SexAI, Sfr274I, SfuI, SgfI, SgrAI, SmiI, SpeI, SplI, SrfI, Sse8387I, SseBI, SstI, StuI, SunI, SwaI, Tth111I, Vha464I, XhoI, XmaIII

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party. Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010