es 1 ea 7 Base Base wo 40, 0H bord S Be ee here Ha M on w * | peowmieose| (* [pipeoxymieose ert nudlgonee tio O14, SO DNA palynerase: ronds nere can't add the next nudeonde ond StOPS. Senger heihes!] __ Template strand S'ATGACCAGATCTGATG 5 ¥ Phmers (abeud PTACTIGG TC TAGACTAC _ mth P32 (adioacnie) _) Primer nce ees \nadere To Enc 1S ADDED TACIGGTC. aa acre eR a iG) nc “excess DNA\tO sequeree ay CTAGASTES Waacte + excess priiners ve al enacre Y voces DNA Ose > Wt re ras Ladd ATP sexcess GATP,ATTP, TACIGGTCTA «5 agn ban? A AGTP, ACTP (ruceotiac) HST ETAGACTA seach tube gets 1. oF cca 4. dTTP FS 4 dideox: TACIOGTCT equ. att? & Sideoxy nucleotide. 1) TReReeTCTAGACT ERS Urreoests A sa SBMA cout Pures ae Se Hic] yo * + bigger fragments rmycless = (fom “ve 40 410) le S + excess CTP, AGTP, ele. ea) Goal the way 1 the other iO ° ia] g Ds eag eeustnct ight toler a |r Vee oe whoa reactor eae tues 40 get end @) end CCAGATCGATA = GGTCTAGACTAC