Human REX1-OCT3/4Nanog Methylation, set of

PCR primers for validation of methylation status of iPSC promoters.

he aim of Alleles Human REX1-OCT3/4-Nanog Methylation primer sets is to provide researches with

tion statuses of the Oct3/4, Rex1 and NANOG promoter regions. In Takahashis 2006 iPS publication, it was discovered that these promotor regions were highly unmethylated. Instead of ordering individual oligos, these complete for 50 reactions for each gene. Each batch of primers is vigorously tested for oligo integrity.

Protocols
PCR protocol (using Allele-in-One Mix):

Box 3 | Recommended PCR Setup

Component Taq DNA Polymerase Template DNA (1ng/l)
M)

Volume 5 units 1l 1 l 1 l 5 l 1 l

Final Conc. NA 20 pg/l 0.6 M 0.6 M 1X 0.2 mM

Features
Pre-tested and complete set of PCR primers for: 3 promotor genes. All sequences are identical to those as published by Takahashi et al. in their 2006 publication in Cell that All oligos were produced in-house at Allele Biotech with quality control provided at uniform concentrations for easy reaction setup.

Upstream primer (20

Downstream gene10 X PCR buffer 10 mM dNTP mix

(20 M)

Nuclease-free water

of 50 l

References
1.

Takahashi, K. and S. Yamanaka, Induction of pluri-

follows:

Denature Anneal Anneal and extend

94C for 30 sec 58C for 30 sec 72C for 1min

Name mehREX1-F1-S mehREX1-F1AS mehOCT3/4 F2-S mehOCT3/4 F2-AS mehNANOG-F1S mehNANOGF1-AS

Primers Sequence GGT TTA AAA GGG TAA ATG TGA TTA TAT TTA CAA ACT ACA ACC ACC CAT CAA C GAG GTT GGA GTA GAA GGA TTG TTT TGG TTT CCC CCC TAA CCC ATC ACC TCC ACC ACC TAA TGG TTA GGT TGG TTT TAA ATT TTT G AAC CCA CCC TTA TAA ATT CTC AAT TA

Box 1 | Product Info Human REX1-OCT3/4-Nanog Methylation Detection Primer Set

CAT# ABP-SC-iPShMSP

Contents
3 pairs of PCR primers (50l of 20M oligo)

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