Anti-RFP Monoclonal Antibody, 3F5 MAb is characterized by

high affinity and specificity against the DsRed derivatives

Anti-RFP Monoclonal Antibody Cat #: ACT-CM-3F5RFP1 Storage Condition: 2-8C for up to 6 months. For long term storage, store at -20C. Content: 100 g, 1mg/ml

Background: Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living protein (GFP) from Aequorea victoria, a red the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc) which become attractive tools for a number of applications. For example the combination of green and red fusion proteins allows multicolor tracking as well as protein-protein interaction studies in living cells. Description: Allele Biotech provides Chromoteks Anti-RFP, which is a mouse monoclonal antibody (MAb) against multiple red FPs, designated as multi-red 3F5. 3F5 corresponding fusion proteins. It is suitable for ELISA, immunoblotting, immunoprecipitation, and and fusions thereof become attractive tools for interacting proteins or enzymatic analyses as well as for microscopic analyses. Features: proteins derived from DsRed and RFP-tagged fusion proteins. - Liquid; hybridoma supernatant. The hybridoma was cultured in protein free medium. - Tissue culture supernatant was concentrated by centrifugation using a 30 kD exclusion membrane to an antibody concentration of 1 mg/ml. - Isotype: IgG2a

Applications: Suitable for ELISA, immunoblotting, assays. Recommended Dilutions: - Western Blot: Used at a dilution of 1/1000. - IP ,Co-IP: 4g of multi-red antibody 3F5 used incubation for 1 h at RT 1/1000. Used at a dilution of

Mouse RFP antibody 3F5 is highly specific to RFPs and shows no crossreactivity to GFP or any other cellular protein primary antibody: 1/1000 RFP antibody 3F5 incubation for 1 h at RT secondary antibody: 1/500 anti-mouse-Alexa647 incubation for 1 h at RT