Population Selection Criteria

LT-HSC SP+ c-Kit+ Sca-1+ Lin -

NK Cells Nk1.1+ CD3-
B-Cells CD19+ 33D1-
naïve CD4+ T-Cells CD4+ CD25- CD69-
activated CD4+ T-Cells Isolated 8-11 Hours After Stimulation CD4+ CD25+ CD69+
naïve CD8+ T-Cells CD8+ CD25- CD69-
activated CD8+ T-Cells Isolated 8-11 Hours After Stimulation CD4+ CD25+ CD69+
Granulocytes Gr-1+ 7/4 clone+ Lin- (CD2,CD5,B220,F4/80,ICAM-1,Ter-119)
Monocytes Mac-1+ FSC and SSC properties
Nucleated Erythrocytes Ter119+ Lin- (CD3, CD4, CD8, Mac1, Gr1, B220)
Tissue Purity
WBM 0.99
PB 0.99
Spleen 0.99
Spleen 0.98
Spleenocytes treated with ConA 0.99
Spleen 0.99
Spleenocytes treated with ConA 0.99
WBM 0.99
PB 0.96
WBM 0.98