ARCA, 5(ppp)5G

Anti-Reverse Cap Analog

Description

n order to carry out large scale synthesis of RNA transcripts by way of in vitro transcription, adjustment of the RNA capping element is suggested. To help researchers accomplish this, Allele offers ARCA (Anti-Reverse Cap Analog), which is a modified cap analog in which the 3 OH group is replaced with OCH3. This re-figuration allows for RNA polymerase to only initiate transcription with the remaining hydroxyl group, and forces ARCA incorporation in the forward orientation. This outcome leads to the transcripts synthesized with ARCA at the 5 end to become translatable causing a stimulatory effect on translation. This feature is not found in standard cap analogs.

Box 1 | Content
Cat. No. ABP-PP-NTPARCA

Quantity

Concentration

50l

100mM

Each batch of reagents is vigorously tested for consistency and stability, and certified 99% pure and free of endonucleases and modifying agents.

Quality Assurance:

Website: www.allelebiotech.com Call: 1-800-991-RNAi/858-587-6645 (Pacific Time: 9:00AM~5:00PM) Email: oligo@allelebiotech.com

For Technical Support: Email:oligo@allelebiotech.com

Box 2 | ARCA Structure

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