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Determination of Heavy Metals in Fruits of Capsicum annuum L.

by Inductively Coupled Plasma Mass Spectrometry Thawatchai Phaechamud,1 Juree Charoenteeraboon,2 and Chutima Limmatvapirat1,*
1

Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand 2 Department of Biopharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand 3 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand
*

chutima@su.ac.th

Keywords: heavy metals; Capsicum annuum; ICP-MS; long red pepper

Abstract. Capsicum annuum L. belonging to the family Solanaceae was a popular spice in Thailand. The mature fruits (long red pepper) were composed of carotenoid, capsaicinoid and ascorbic acid exhibiting physiological and pharmacological properties. Because of environmental pollution, these fruits might be contaminated with toxic heavy metals. Therefore, the objective of this study was to determine the concentrations of 11 heavy metals in long red pepper using nitric acid digestion followed by ICP-MS analysis. 13 samples of fresh fruits were collected in 9 provinces located in the west of Thailand. The concentrations of toxic heavy metals including arsenic (As), cadmium (Cd), mercury (Hg), and lead (Pb) in all samples were lower than the permissible limits (4, 0.3, 0.02, and 10 mg/kg, respectively) while the concentrations of essential elements including copper (Cu) and zinc (Zn) were less than the permissible limits (20 and 100 mg/kg, respectively). The highest concentrations of aluminium (Al), chromium (Cr), iron (Fe), manganese (Mn), and nickel (Ni) were found 36.813, 0.345, 44.464, 32.172, and 1.623 mg/kg, respectively. The concentrations of heavy metals presented in this study could be valuable in quality control of vegetables for Thai people.

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