Heat shock proteins

.......................................................................
.. ,
, .. 2555

, ,

Heat
shock

proteins

7 Hoagland
PEG

6000

Hoagland
PEG

6000 5

4
Hoagland PEG

6000 5

 Hoagland
PEG

6000 5

Hoagland
PEG

6 0 0 0

Heat

shock

proteins 6 HSP 80.2, HSP 78.4, HSP 71.1,

HSP

58.7, HSP

26.7

HSP

17.2

Semi-Quantitative RT-PCR
Heat shock
proteins Actin

housekeeping

gene

Normalization

RT-PCR Actin

Title

Study on growth and the expression of Heat

Shock Protein genes in
drought condition of SANG YOD rice (Oryza

sativa)
Author

Chanida Imccaran

Advisor

Dr. Supaporn Passorn

Academic Paper

Independent Study B.Sc. (Biotechnology)

in Biotechnology,
University of Phayao, Year 2012
Keywords

Drought condition, Heat shock proteins, SANG

YOD rice

ABSTRACT
Study on growth and the expression of Heat Shock
Proteins in drought condition of SANG YOD rice (Oryza sativa).
As an experimental control, the seedlings were germinated and
grown for 7 days then continue growing in liquid medium
Hoagland solution without PEG 6000. To simulate drought
condition,

the

plants

were

cultured

in

liquid

medium

supplemented with 5 % (w/v) of PEG 6000. The cultured period

was 4 weeks. The result showed a significantly decreased in
height of seedlings grown in liquid medium supplemented with
5 % (w/v) of PEG 6000 and appearance of bent and wither
leaves as well as dwarfed trunk was observed. These results
concluded that 5 % (w/v) of PEG 6000 has negative effect to
the development of seeding in term of height and defected

forms of leaves and dwarfed trunk. Gene expression of six heat

shock proteins including HSP 80.2, HSP 78.4, HSP 71.1, HSP

58.7, HSP 26.7 and HSP 17.2 genes were compared using
Semi-Quantitative RT-PCR method. The results could not be
concluded confidently due to the expression of Actin gene,
which was chosen to be as internal standard for quantitative
comparison of mRNA levels, failed to detect. The result
suggests that the RT-PCR amplification condition for Actin
gene was probably unsuitable.