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MORPHOLOGY
entire margins umbonate elevation. Gram negative motile bacillus shape mucoid colonies
CULTURE facultative anaerobe grown on nutrient agar 48 h at 25C. Optimum growth: pH 9 and at temperatures from 20-37 C.
SEROLOGY
difficult to treat because of resistance to a variety of antibiotics P-Lactam resistance Aminoglycoside resistance
BIOCHEMICAL TEST
oxidase test (positive) negative for methyl red test. Reduce nitrate Indole negative Simmon's citrate positive
ESCHERICHIA
a) Morphology
b) Culture
d) Serology test
ENTEROBACTER
1)morphology
-gram negative rod -capsulated -motile -Straight rod shape. -0.6-1.0 micrometers wide X 1.2-3.0 micrometers long.
2)Culture
-Facultative anaerobe. -Best results are obtained when Enterobacter cultures are incubated at 30C. At 37C.
3)Biochemical test
-Indole negative. -Lysine negative (except E. gergoviae). -Voges-Proskauer positive (mostly). Simmons citrate positive (mostly).
4)Serology test
PROTEUS :
CULTURE Facultative anaerobes Do not form endospores Exhibit swarming behavior - form concentric rings as they spread across the surface of agar plates.
BIOCHEMICAL TESTS Oxidase negative Catalase positive Nitrate positive Urease positive Phenylalanine deaminase positive
PROVIDENCIA
BIOCHEMICAL TESTS : Citrate utilization - Positive d-Mannose fermentation - Positive Gelatin liquefaction (22C) -Negative H2S production (TSI) - Negative Lipase (corn oil) - Negative Ornithine decarboxylase - Negative
Swarming - Negative
MORGANELLA
MORPHOLOGY
Straight rods, 0.6-0.7 um in diameter and 1.0-1.7 um in Motile by means of peritrichous flagella large,gray, moist colonies
CULTURE
grow on nutrient agar,MacConkey agar, trypticase soy agar. Facultatively anaerobic Gram-negative
SEROLOGY
The 0 antigens were used for grouping the H antigens were used for further subclassification.
BIOCHEMICAL TEST
catalase :+ve gelatinase : -ve oxidase : -ve VP : -ve indole : +ve urease :+ve
CITROBACTER
Morphology
Belong to gammaproteobacteria Gram negative Non-spore forming Rod-shaped bacteria Mostly surrounded by flagella Motile Facultative anaerobes Culture
Most will grow in wide temperature range in ordinary culture media including nutrient agar and blood agar. On BA, enterobacteria produce large, shiny, grey colonies that may be haemolytic. A coliform & fecal coliform
Biochemical test
Indole test: posite. Ability to convert tryptophan to indole. ferment lactose, and use malonate.
Serology test
K.pneumoniae
morphology
- gram ngtve bact -non motile -slimy grayish white colonies on NA/muccoid appearance -small size ( 1-2 um) coccobacilli capsulated -arranged in pairs n cluster
culture
-facultative anaerobes -growth on NA - produce distinctive yeasty odor serology test - prodce nzyme (carbapenemase) makes it ressintant to drug carbapenem -inhibit growth of similar related strains bact by prodcng proteinaceous toxins