Stem Cell Markers

Stem cell biology, the study of mechanisms causing the undifferentiated embryonic
or adult stem cells to differentiate, is one of the fastest growing areas in biology
today. Cell Signaling Technology's goal is to accelerate stem cell research by
developing the highest quality, most rigorously validated antibodies for the study of
stem cell markers, epigenetic regulation and stem cell signaling.

Ternary complex of the DNA binding domains of Oct1 (red) and Sox2 (yellow) bound
to cognate DNA (in this case a region of the HoxB1 regulatory element).
New No. Antibody Application Reactivity Lineage Stage Description
Five-transmembrane
glycosylated cell-surface
blastocyst,
CD133 early protein initially described as
hematopoietic
(C24B9) W, IP, IHC-P, embryo, a hematopoietic stem cell
new 3663 H stem cells,
Rabbit F mesoderm, marker. Also expressed in
neuronal stem
mAb ectoderm and used to identify
cells
neuronal, cancer and
embryonic stem cells.
Transcription factor
expressed in undifferentiated
pluripotent embryonic stem
cells and germ cells during
Oct-4 W, IHC-P, early
new 2750 H blastocyst normal development.
Antibody IF-IC, F embryo
Together with Sox2 and
Nanog, is necessary for the
maintenance of pluripotent
potential.
Mediates biological effects by
binding to and activating c-
kit. Promotes proliferation of
SCF early
new 2273 W, IHC-P H blastocyst embryonic stem cells and
Antibody embryo
hematopoetic stem cells.
Essential for hematopoiesis,
melanogenesis and fertility.
Transcription factor
expressed in undifferentiated
pluripotent embryonic stem
cells and germ cells during
Sox2 early
new 2748 W, IP H, M blastocyst development. Together with
Antibody embryo
Oct-4 and Nanog, is
necessary for the
maintenance of pluripotent
potential.
GFAP neural stem
(GA5) W, IP, IHC-P, cell/ Marker protein specifically
new 3670 H, M, R ectoderm
Mouse IF-F ectodermal expressed in astrocytes.
mAb progenitor
Cell-adhesion molecule
important in cell adhesion
bulge stem
and migration. Marker
Integrin β1 H, M, R, cell/
new 4706 W ectoderm expressed on mesenchymal
Antibody Mk, Hm mesenchymal
stem cells, bulge stem cells
stem cell
and during development of
the liver.
new 2274 MELK W, IP H, M ectoderm neural stem Implicated in stem cell
Antibody cell renewal, cell cycle
New No. Antibody Application Reactivity Lineage Stage Description
progression and pre-mRNA
splicing. Marker for neural
progenitors.
RNA-binding protein that
regulates the translation of
target mRNA during
asymmetric cell division of
Musashi neural stem ectodermal precursor cells.
new 2154 W, IF-F H, M, R ectoderm
Antibody cell Marker for proliferating
neural precursor cells.
Expressed in epithelial stem
cells including intestinal and
mammary gland stem cells.
Glycoprotein found in serum
of mammalian fetal liver, yolk
sac and GI tract. Expressed
AFP hematopoietic
new 2137 W, IP H, M endoderm during development of the
Antibody stem cell
primitive endoderm. Marker
for endodermal differentiation
from pluripotent stem cells.
Adhesion molecule
expressed in embryonic (not
VE-
hematopoietic adult) hematopoietic stem
new 2158 Cadherin W, IP, IF-IC H, B mesoderm
stem cell cells. Also expressed in
Antibody
vascular smooth muscle
cells.
Transmembrane
CD34 glycophosphoprotein
(ICO115) hematopoietic expressed in hematopoietic
new 3569 IHC-P, F H mesoderm
Mouse stem cell skin cells, vascular
mAb endothelium and some
fibroblasts.
Multidomain membrane
glycoprotein highly
expressed in some human
cancer cells and in both
CDCP1 hematopoietic
new 4115 W, IP, IF-IHC H mesoderm typical and atypical
Antibody stem cell
(cancerous) colon. May be
epigenetically regulated.
Putative hematopoetic stem
cell marker.
c-Kit hematopoietic Receptor tyrosine kinase that
new 3392 W, IP H, M mesoderm
Antibody stem cell plays a critical role in cell
c-Kit growth and proliferation in
(Ab81) hematopoietic hematopoietic stem cells,
new 3308 W, IP, F H mesoderm
Mouse stem cell mast cells, melanocytes and
mAb germ cells. Binding of its
c-Kit ligand (SCF) to c-kit initiates
(Ab81) proliferation and
Mouse hematopoietic differentiation of
new 3310 IF-IC, F H mesoderm hematopoietic progenitor
mAb (Alexa stem cell
Fluor® 488 cells. Hematopoietic stem
Conjugate) cell marker.
PU.1 W, IP, IHC-P, hematopoietic Member of the Ets family of
new 2266 H, M, (Mk) mesoderm
Antibody IF-IC, F stem cell transcription factors that
PU.1 (9G7) plays a pivotal role in the
W, IP, IHC-P, hematopoietic
new 2258 Rabbit H, M, (Mk) mesoderm differentiation of myeloid
IF-IC, F stem cell
mAb cells and lymphocytes.
new 2216 PU.1 (9G7) F H, M mesoderm hematopoietic Expressed in several
New No. Antibody Application Reactivity Lineage Stage Description
Rabbit hematopoietic cells including
mAb (Alexa B lymphocytes,
stem cell
Fluor® 488 macrophages, neutrophils,
Conjugate) mast cells, early erythroid
cells and megakaryocytes.
PU.1 (9G7) Critical for both
Rabbit determination of
hematopoietic hematopoietic cell lineage
new 2240 mAb (Alexa F H, M mesoderm
stem cell and regulation of
Fluor® 647
Conjugate) differentiation vs. stem cell
proliferation.
Basic helix-loop-helix
transcription factor that
functions as a master
regulator of embryonic
morphogenesis. Plays
essential roles in
mesenchymal differentiation
TWIST1 neural crest and osteogenic
new 4119 W, IP H, Mk
Antibody stem cell determination. Also plays
critical role in neural crest
formation and is a marker for
neural crest stem cells.
Upregulated in various
human tumors and may play
a role in EMT and
metastasis.