UNIVERSITY OF AGRICULTURAL SCIENCES, BANGALORE

SET-1

SERICULTURE COLLEGE, CHINTAMANI- 563125

Final Theory External Examination/Main or Supplementary ID: NO--------------TITLE: PLANT BIOTECHNOLOGY PBT201 (1+1) II B.Sc. (Agri.) IISEM-- 2011-12 MAX.MARKS: 50.0M TIME: 2.00 hr

Q.1a

Underline the most appropriate answers

(10X0.5M=5M)

1) Which one of the following bacteria has found extensive use in genetic engineering work a) Agrobacterium tumifaciens b) Clostridium septicum c) Xantomonas citri d) Bacillus coagulens 2) What is the name of technique used to obtain Heterokaryon a) Interspecific hybridization b) intergeneric hybridization c) Somatic hybridization d) intervarietal hybridization 3) Which is the precursor for the biosynthesis of Auxins a) Mevalonate b) Tryptophan c) IPP d) All the above 4) The plant hormone responsible for breaking of the dormancy in plants is by a) IAA b) GA3 c)Kinetin d)None 5) The additional round of DNA replication is called a) Duplication b) Clone c) Endo-reduplication d) Replication 6) Which one of the following is not a PCR-based molecular marker a) RAPD b) AP-PCR c) DNF d) RFLP 7) The Selectable marker that provides Rsistance to the Antibiotic kanamycin is a) Gentamycin acetyl transferase b) npt-II c) hpt-II d) Streptomycin phosphotransferase 8)A Vector specifically designed for cloning 40-49 kb DNA fragment is a) Plasmid b) Phagmid c) Cosmid d) BAC 9) Virus free plants can be produced by a) Ovule culture b) Anther culture c) Stem tip culture d) Leaf explants culture 10) Undifferentiated mass of cells is a) Cell clumps b) Callus c) Both d) None

Q.Ib Match the following

A a) PCR-Technology b) DNA Finger printing c) Pseudomonas d) Embryo culture e) Protoplast fusion f) Molecular Scissors g) Sorbitol h) Cell viability i) Wilmut j) Shoot bud B Unipolar Hanning Dolly Osmaticum Somatic hybrid FDA Kary Mullis Patenting organism Restriction enzymes Allec Jeffrys

(10x0.5M=5M)

Q.II

Answer the Following Questions in one or two sentences

(10X1M=10M)

1) Micropropagation 2) Cybrids 3)Transformation 4) Binary vector 5) Gene cloning 6) Ti Plasmid 7) Sterilization technique 8) Plant growth regulator 9) Haploid plants 10) Suspension culture

Q.III
1) 2) 3) 4) 5) 6) 7) 8)

Answer any Five questions

(5x2M=10M)

Define plant Biotechnology and write its major applications Write important differences between shoot bud and somatic embryo What is DNA Finger printing and its application in plants Write a neat labeled diagram of a typical plant gene Mention the role of Auxins and Cytokinins in plant tissue culture Essentials required for Gene cloning What is direct and indirect method of regeneration Write important features of a vector

Q.IV Answer any Four questions

1) 2) 3) 4) 5) 6)

(4X2.5M=10M)

List out direct and indirect gene transfer methods List important Selectable and Scorable marker genes What are Restriction Enzymes? Describe type I, II and type III REs What are somoclonal variations? Briefly describe their molecular basis and applications What is a Marker describe various types of markers What is PCR Technology , procedure and its applications

Q.V Answer any three questions

(4TH Question is must)

(3+3+4=10M)

1) Briefly describe the modes of production of haploid plants and their various applications for crop improvement 2) Describe Southern technology 3) What is DNA sequencing? Describe Sangers method of DNA sequencing 4) Agrobacterium mediated gene transformation ----------------------------------------------------------------GOOD LUCK--------------------------------------------------------

UNIVERSITY OF AGRICULTURAL SCIENCES, BANGALORE

SET-2

SERICULTURE COLLEGE, CHINTAMANI- 563125

Final Theory External Examination/Main or Supplementary ID: NO--------------TITLE: PLANT BIOTECHNOLOGY PBT201 (1+1) II B.Sc. (Agri.) IISEM-- 2011-12 MAX.MARKS: 50.0M TIME: 2.00 hr Q.1a Underline the most appropriate answers (10X0.5M=5M)

1) Which one of the following technique is employed to detect proteins of a particular specificity a) Western blotting b) Southern blotting c) Northern blotting d) Slot blotting 2) The plasmid derived from E.Coli Plasmid ColEI is a) YIP b) pYAC c) PBR-322 d) Ti Plasmid 3) Which is the precursor for the biosynthesis of Ethylene a) Mevalonate b) Methionine c) IPP d) All the above 4) The plant hormone responsible for breaking of the dormancy in plants is by a) IAA b) GA3 c)Kinetin d)None 5) The additional round of DNA replication is called a) Duplication b) Clone c) Endo-reduplication d) Replication 6) Which one of the following is not a reproducible molecular marker a) RAPD b) AP-PCR c) DNF d) RFLP 7) Who is the following is associated with the discovery of Restriction Endonucleases a) H.Smith b) H.G. Khorana c) Nierenberg d) None 8)A Vector specifically designed for cloning 10 kb DNA fragment is a) Plasmid b) Phagmid c) Cosmid d) BAC 9) Virus free plants can be produced by a) Ovule culture b) Anther culture c) Stem tip culture d) Leaf explants culture 10) Plants raised from the pollen culture are a) Diploid b) Tetraploid c) Haploid d) Polyploid

Q.Ib Match the following

A a) PCR-Technology b) Jumping genes c) Pseudomonas d) Embryo culture e) Protoplast fusion f) r-DNA Technology g) Monnitol h) Cell viability i) Venter j) Somatic embryo B Bipolar Hanning HGP Osmaticum Somatic hybrid TTZ Kary Mullis Patenting organism Restriction enzymes Mc Clintock

(10x0.5M=5M)

Q.II

Answer the Following Questions in one or two sentences

(10X1M=10M)

2) Totipotency 2) Cybrids 3)Transformation 4) Shuttle vector 5) Genetic engineering 6) Dis arming 7) Cryopreservation 8) Plant growth regulator 9) Haploid plants 10) Electrophoresis

Q.III
1) 2) 3) 4) 5) 6) 7) 8)

Answer any Five questions

(5x2M=10M)

Define plant Tissue culture and write its major applications What are the different types of media and its major and minor nutrients What is DNA Finger printing and its application in plants Write a neat labeled diagram of a typical plant gene Mention the role of Gibberellins and Cytokinins in plant tissue culture Essentials required for Gene cloning What are symmetric and Asymmetric hybrids Write important features of a Plasmid

Q.IV Answer any Four questions

1) 2) 3) 4) 5) 6)

(4X2.5M=10M)

List out direct and indirect gene transfer methods Ballistic gene gun method What are Restriction enzymes? Describe type I, II and type III REs What are somoclonal variations? Briefly describe their molecular basis and applications Write important applications of molecular markers What is PCR Technology, procedure and its applications

Q.V Answer any three questions

(4TH Question is must)

(3+3+4=10M)

1) Briefly describe the modes of production of haploid plants and their various applications for crop improvement 2) Describe Various methods of protoplast fusion 3) What is DNA sequencing? Describe Maxum-Gilbert method of DNA sequencing 4) Agrobacterium mediated gene transformation ----------------------------------------------------------------GOOD LUCK--------------------------------------------------------