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General account , structure and functions of bacterial cells : (NOTE : SHORT QUESTIONS CAN BE IN THE FOUND OF BOLD LETTERS IN THE NOTES) Anton Von Leeuwenhoek is regarded as father of bacteriology because he first time (1683) observed these tiny animalcules from the scum of teeth of a man. Bacteria are cosmopolitan in distribution, occur every where in water , air, soil, food stuffs etc. Bacteria are prokaryotic, achlorophyllous and u nicellular organism. Instead of true chlorophyll, bacteria may contain bacterio chlorophyll and chlorobium chlorophyll. Normally, the bacteria may ranges between 1 to 5 um. It posses the foloowing forms: Cocci(spherical) Eg.Micrococcus, Diplococcus Bacilli(rod shaped)- Eg. Diphobacilli, Streptobacilli Spirilla (spirally coiled) Eg. Spirochaete, Spirillum Vibrio(comma shaped) Eg. Vibrio cholera
On the basis of flagella , bacteria are : Atrichous(no flagella ) Monotrichous(single flagellum at each end) Amphitrichous(one flagellum at each end) Cephalotrichous(tuft of flagella at each pole) Lophotrichous(tuft of flagella at each pole) Peratrichous(several flagella all over the surface)
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Saprophytic bacteria : These types of bacteria take their food from dead organic matter and are found growing in animals dung decaying vegetables etc. It secretes and breaks the complex food into simpler and soluble forms.
Symbiotic bacteria : Some bacteria like Rhizobium species occur as symbionts on the root nodulues of leguminous plants. (Note :- Symbiotic means both the partners or the organisms are equally benefited by the symbiotic bacteria.)
The energy is either obtained chemically called chemotropic or from solar radiation called photo autotrophic forms. While manufacturing food, chemoautotrophic bacteria obtain energy from
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chemicals; do not require solar energy but the photoautotrophic (photosynthetic) bacteria posses chlorobium chlorophyll and bacterio chlorophyll which trap solar energy. Bacterial photosynthesis takes in light but in absence of oxygen. Kochs postulates a. The specific organism should be shown to be present in all cases of animals suffering from a specific disease but should not be found in healthy animals. b. The specific micro organism should be isolated from the diseased animal and grow on artificial laboratory media. c. This freshly isolated micro organism when inoculated into a heat laboratory animal should cause the same disease seen in the original animal. d. The micro organism should be reisolated in pure culture from the experimental infection.
Gram positive bacteria They retain the deep blue or purple with crystal violet and subsequently with potassium iodide and alcohol. The cell wall is 100 to 200 0A. Peptidoglycan constitutes the 70% of cell wall. They are generally susceptible to lysosomes or antibiotics. Generally they do not bear fimbriae or sex pili. Cell membrane forms mesosomes.
Gram negative bacteria They do not retain the color when treated with potassium iodide and alcohol. The cell wall is 70 to 1200A. Peptidoglycan constitutes the 30 % of the cell wall. They are generally resistant to lysosomes or antibiotics. Generally they bear pili or fimbriae. Mesosomes are absent.
Economic importance
(Note : While writing the economic importance example i.e scientific names are compulsory.) Beneficial activities :a. Some species of bacteria like Pseudomonas, Rhizobium, Chlostridium ,Azotobacter, fix atmospheric nitrogen in usable forms for plants. b. Some species of clostridium are used in manufacturing of Vitamin B(riboflavin). c. Lactic acid bacteria (Streptococcus lactis) help in coagulation of milk. d. Three types of medicine antibiotics, antisera and vaccines are formed by bacteria. e. Bacteria (E. coli) present in human colon synthsize vitamin B and releases it form human use. f. Bacteria help in digestion in herbivores animals in the digestion of cellulose. Bacillus subtilis produces the enzymes amylase and protease. g. By the activity of clostridium the butyl alcohol and acetone are obtained form sugar molasses. h. It helps in fibers retting of jute, coconut and other fibrous plants.
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i.
Bacteria like Bacillus subtilis, Bacillus ramosus etc. decompose complex organic compounds like proteins into ammonium compound . These are also known as ptrefying bacteria.
Harmful activities :a. Many human diseases such as diarrhoea, meningitis, pneumonia etc. are caused by pathogenic bacteria. b. Some denitrifying bacteria reduce the soil fertility by depleting the nitrogen contents of soil. c. Some bacteria spoils food stuffs, leather and wooden articles. d. Certain bacteria destroy the activity of penicillin by producing an enzyme penicillinase. e. Tuberculosis of cattle, anthrax of sheep is caused by Mycobacterium tuberculosis and Bacillus anthracis respectively. f. Citrus canker by Xanthomonas citri, ring rot of potato by cornebacterium sepedonieum etc. g. Spirochete cytophoga destroys cotton and article made from it.
HSEB QUESTIONS
VERY SHORT QUESTIONS: Give the function of mesosomes of a bacterial cell. How do the bacteria get their nutrition? ANSWER IN BRIEF : Give the economic importance of bacteria. Discuss the agricultural economic importance of bacteria. (3 Marks each) (1 Marks each)
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