Professional Documents
Culture Documents
Introduction
My interest in research safety began after skimming the recommended safety precautions for working with certain chemicals in the 7.02 lab. Previously, research safety seemed like a non-issue, since everyone agrees that people should be cautious with dangerous substances and try to minimize contact as much as possible. After reading the precautions and observing how one particular chemical, ethidium bromide, was actually handled in lab, I saw that the research safety was not as black and white as I previously assumed. People do not generally agree on how to handle ethidium bromide, and while the recommended safety precautions are very clear, no lab I spoke with actually followed those guidelines. Instead, decisions about how to treat a chemical were influenced not only by the formal guidelines, but by the examples set by coworkers, the attitudes of the supervisor, the frequency with which the chemicals were used, and by the individual's willingness to take risks. Research safety is not simply a matter of education and implementation; researchers have to asses the hazards of working with a chemical, and use their own professional judgment on how to act.
1. Motivation
After looking over my 7.02 lab safety sheet describing ethidium bromide (or EtBr), I was worried about our practices in the lab. (See Appendix A) I saw people touching the gels, which are stained with EtBr, with gloves and then touching other common objects in the lab, such as faucets and beakers. I also saw people dumping the gels in the garbage while the TA's watched. As a precaution I began wearing gloves all the time and washing my hands after leaving lab. Why didn't they mention something in lecture or recitation about how dangerous this ethidium bromide was? Was all this dumping a serious problem? To address these concerns, I interviewed an instructor in the 7.02 lab for my mini-project. In general, she seemed to take the problem very seriously, and said she felt there should be more education about these chemicals before lab in recitation. I decided to follow up on this issue for my final project. Instead of concentrating on educational labs, I shifted the focus to research labs to see how the problem is dealt with in a less controlled environment, where no TA's are present to make sure people don't violate safety precautions.
2. Scenario
I am a new researcher in this laboratory, and part of my job is to prepare and run polyacridamide gels. I read the descriptions of the hazardous reagents involved in this procedure, and I found that the ethidium bromide used to stain the DNA is a strong carcinogen. I also noticed that acetone (a component of nail polish remover) was listed as a dangerous substance, and should never be breathed or touched. As I am working, I notice several other employees touching the gels containing ethidium bromide and then touching faucets or door handles. I also notice people disposing of the gels in the garbage instead of into the collection jar in the hood. What should be done in this situation? 1. Who do I contact first? 2. Do you see this situation as a legitimate safety hazard? 3. What should be done about the ethidium bromide that has been poured down the drain or thrown in the garbage? 4. Who decides what the unsafe disposal limit is? 5. Should the community be informed? When? 6. What is OSHA? How are they involved in this lab? 7. Is the lab inspected? By whom? 8. Who determines and enforces the safety regulations? 9. Do you trust that the lab's door handles and faucets are free from hazardous substances? 10. Where do you get information about dangerous substances? 11. How much faith do you place in this information? 12. How are new employees educated about safety practices? What is mentioned about ethidium bromide? 13. Do you have a list of emergency numbers? 14. When is the last time a number was called?
3. Interviews
People had a variety of opinions about how to handle EtBr, and I tried to get a feel for how they were educated about its use, and what factors influenced their decisions in how to handle it. The main barrier I encountered in these interviews was that the researchers were sometimes cautious about discussing their safety practices, as if they suspected I was a disguised OSHA representative. I had to reword some questions in order to get direct answers. For instance, the last question I asked about the safety numbers was intended to get the interviewee to tell a story about an emergency or a safety problem and how the lab handled it. I never got a detailed response if I simply asked, "what are some examples of safety concerns you've had in the past?" Overall, people were very helpful in answering my questions once I had proposed them correctly.
office or the lab safety coordinator. She did know about the radiation office however, and said if there was another kind of emergency she'd probably just call them first. From her experiences in other labs, she said the P.I. "sets the tone" as far as safety is concerned. Martha had had no formal training, but remarked that "its a matter on the job training" and that you have to use your own judgment sometimes as well. Concentration and ventilation are two guidelines she uses when making these decisions, as well as looking at what other people do.
4. Discussion
How does an individual asses the hazards of working with a particular chemical? Why is ethidium bromide described in safety literature as a dangerous substance, never to be disposed of in the garbage if it is done regularly? Dismissing the 7.02 safety guidelines as overexaggerations propagated by OSHA and the 7.02 staff is an easy explanation for these incongruities. However, unlike most carcinogens, the method by which EtBr causes cancer is actually known. It has a flat, ringed structure, and has residues that can form weak bonds with portions of DNA. When it comes in contact with DNA, it can slide between two of the rungs of the DNA "ladder" and hinder replication. Its presence greatly increases the chance that mistakes will be made when the DNA is copied before the cell divides. The more mistakes made in replication, the more of a chance one of those mistakes will give rise to a cell that cannot regulate its division, giving rise to cancer. When I learned this, I suddenly became much more concerned about ethidium bromide. I pictured a molecule of EtBr intercalating in my DNA rungs, changing the usually reliable process of replication, and mutating my cells. The idea is certainly frightening, and because of this I personally cannot simply dismiss the safety precautions described in the 7.02 guidelines. After talking with researchers, I have seen several steps that one could take to address a safety concern about EtBr, but the dilemma here was in deciding when something is really a risk. The range of responses was considerable-I was very surprised to find that Brenda considered throwing the gels in the trash "standard practice", while Paul said he would quit if something wasn't done. If I had some more time, I would have liked to interview a specialist on carcinogens to see how dangerous he or she thought the EtBr was in the concentrations being used in these gels. I expected my scenario to be something that would happen once in a while in a lab, but it seems that in most labs it happens all the time and isn't even considered a safety risk. After these interviews, two possible causes for this attitude about EtBr I noticed were desensitization to everyday dangers, and pressure to conform with coworkers. In my interviews, particularly in Brenda and Martha's lab, I had the impression that the newer workers were generally more afraid of EtBr than people who had been there for a while. Brenda especially seemed very desensitized to the EtBr she dealt with almost daily. My explanation for this is that is it probably extremely difficult to perceive something as a risk when it looks like water (poses no immediate physical danger), and you see it every day. Also, radiation is taken so seriously it almost seems like the only important safety hazard. Researchers who took extra precautions were seen as inexperienced because they had not been desensitized to these risks. This would seem to create some pressure for those people to dismiss their ideas about what is dangerous and conform to the practices of coworkers. Coworkers teach each other more about safety practices than OSHA guidelines or chemical labels, and if someone in charge has the wrong idea about howdangerous something is, they may influence others in the same way. Overall, I feel that this project has been very informative not only about the safety networks available to researchers, but about how laboratories are run and managed. One problem with my study is that the only profit-based lab I could get an interview in was also a biomedical lab, not a genetic research lab like the academically-funded labs I interviewed. However, I did see some differences which I feel might have something to do with the funding source. First, the academic labs appeared more autonomous than the biomedical lab, and seemed to handle their affairs privately as much as possible. At the hospital, Paul was very concerned with both OSHA and the FDA, while at the Medical Schools the researchers told me that the P.I. has considerable influence over safety practices and was not influenced directly by any agency. Martha didn't even know what OSHA was until I told her. Since the profit-based lab had to meet FDA guidelines to make their work worthwhile, they had a built-in interest in safety. On the other hand, the company has a larger interest in making a profit, and safety concerns seemed to be there for the FDA more than for the workers. Also, the lab employees at the hospital were in the dark about exactly what they were researching. The way the labs are managed does seem to have some implications in how they handle safety.
listed for EtBr on its package, and to work to encourage everyone in the lab to do the same. In my opinion, the person involved should speak with coworkers first, then the supervisor, the safety officer, and finally go outside the lab to report to the safety office. Consequences of this action are not trivial; it would potentially bring the distrust and scorn of coworkers, since they would probably see the changes as an inconvenient and unnecessary. In order to avoid these problems, I think the government should work to enforce their regulations even more, especially in academically funded labs. While I realize this would create more inconveniences, someone has to take responsibility for keeping track of the total amount of chemicals that end up in our landfills and down the drains. Also, researchers need some motivation to keep their respect for carcinogens they use daily, and prevent these hazards from failing into the shadow of the radiation dangers. In summary, decisions about how to handle a chemical were influenced not only by what is printed on the box or in safety guidelines, but also to a large extent by examples of coworkers, willingness to assume personal risk, desensitization to everyday dangers, and the attitude of supervisor. Safe practices are not always obvious, and people must use their own professional judgment about what is safe for themselves and for the environment.