GENOHEL X

B I O L A B S

GENOHELIX BIOLABS

A part of Jain Group of Institutions

About GENOHELIX About GENOHELIX

GENOHELIX BIOLABS (Formerly JIVAS) was established in the year 1998 with the objective of providing needbased and affordable quality training in the different disciplines of life science . It was a vision of a true forerunner; Dr. R Chenraj Jain, Chairman of Jain Group of Institutions (JGI) who kindled the thought for need based education. Young and vibrant minds are made mundane by piling up of mere theory rather than practice. In such a scenario, Genohelix has taken a different route to embark upon quality training. This centre recognizes the needs of Industries and supplies them with the talent nursed and groomed by experienced professionals. Bridging the gap between Industry and academia is the focal point of all efforts at Genohelix. The industry relevant approach is also the primary vision that circumscribes the implementation of every training and related activities. The technical team of Genohelix comprises of professionals endowed with a blend of industrial skills, research experience and teaching flavor. The rapidly mechanizing and high tech areas of life sciences require an equally advanced infrastructure which is clearly evident in our state of the art laboratories. Our lab equipments amount to a level which exceeds training capabilities to touch and excel in the next realm of research activities and contract research for clients. Our labs are equipped with instruments like 3 litre fermenter, HPLC, Thermal Cycler, Liquid Chromatography system, Automated 7 Sampler UV Visible Spectrophotometer, ELISA Microplate Reader and Washer, Gel Documentation System, Level II Biosafety Cabinets, Pilot Shaker Incubators, Millipore Water Purification System, Ultra cool Centrifuges, Deep Freezers, Protein and DNA Electrophoresis units, apart from all the required basic laboratory instruments. GENOHELIX has an excellent library with over 2000 books, journals, periodicals, ebook collection and CD ROM based data bases built with special care to give the best study ambience. It is truly a knowledge paradise for students with a thirst for intellectual

P R O G R A M S
PROFICIENCY COURSES (15 - 20 Days)
Proficiency courses are basically designed to make the students proficient in the different techniques used in Biotechnology. The students will be exposed to advanced areas of bioscience which will give them a cutting edge in enhancing the employment opportunity but also the academic advancement. The program lays more emphasis on hands on training. It gives a thorough practical exposure in handling the most principally used techniques in different avenues of Biotechnology.

Modules offered:
AGRICULTURAL BIOTECHNOLOGY Isolation of o Cellulose degrading Microbes o Pectin degrading Microbes o Starch degrading Microbes o Lignin degrading Microbes Isolation of Rhizobium from Root Nodules of legumes Isolation of non-symbiotic Azotobacter from Soil Isolation of Associative Nitrogen fixer - Azospirillum Demonstration of Cyanobacterial Symbiosis in Azolla Demonstration of Nitrate reduction by Bacteria Assay of Biofertilizers Mushroom cultivation Estimation of Protein and carbohydrate content of mushroom Vermi-composting FOOD MICROBIOLOGY Isolation of Microbes from fermented foods Study of Aflatoxin producing fungi- Aspergillus flavus Isolation of microbes from infected fruits and vegetables Isolation of microbes from dairy products Quality analysis of water by MPN test Production of Bio-yoghurt Quality analysis of milk Quality analysis of canned food Antimicrobial compounds in food Enumeration of microbes in food by Haemocytometer Production and estimation of Alcohol from Yeast HACCP concepts

IMMUNOLOGY Blood collection, separation of serum and plasma Separation of serum proteins on native PAGE Blood Smear preparation and Visualization of Blood Cells Blood Grouping Counting of RBCs, WBCs, and Platelets Estimation of Haemoglobin using haemometer Erythrocyte sedimentation rate count Counter Current Immunoelectrophoresis PCV Widal, RPR, RA, and Latex agglutination test Measurement of Serum Albumin Separation of Serum Amino acids by Paper Chromatography RID, RIEP and ODD , GENOMICS Genomic DNA isolation from Bacteria Genomic DNA isolation from Plants Genomic DNA isolation from Blood Plasmid DNA Isolation RNA Isolation from Yeast Agarose Gel electrophoresis Gel Elution Restriction Digestion Ligation Polymerase Chain Reaction Phage Titration Transformation Softwares and servers for Data Mining PROTEOMICS Introduction to Protein Biochemistry Structure, Classification and Importance of Proteins Chromatography - Basic principles Types of Chromatography Introduction to Liquid Chromatography system Operation of the LC system Purification of Industrially important Proteins using Liquid Chromatography Introduction to Softwares in Proteomics Analysis of the proteins using tools of proteomics

PLANT BIOTECHNOLOGY Stock solution and Chemical preparation Media preparation In vitro culturing technique Primary Culture: Seed, Embryo, Nodal, Internodal, Leaf Storage organ, Nucellus, Anther, Shoot tip, Root tip Suspension culture Sub culture : Callus, Shoot induction, Root induction, Embryo induction Production of Synthetic seeds Agrobacterium mediated transformation Protoplast isolation Genomic DNA isolation from plant sample CLINICAL BIOCHEMISTRY

CHROMATOGRAPHY Paper Chromatography Thin Layer Chromatography Ion exchange Chromatography Gel Filtration Chromatography Affinity Chromatography HPLC Analysis of pharmaceutical formulations

BIOINFORMATICS Qualitative analysis of Urine Estimation of Blood Glucose Serum Albumin - Globulin ratio Serum SGOT, SGPT, LDH RNA Cholesterol Lipid Profile test Thin Layer Chromatography Assay of Enzymes for the effect of pH Enzyme Concentration Activators INDUSTRIAL BIOTECHNOLOGY Isolation, Screening and Mass production of industrially important micro organisms Enzyme producing microorganism Antibiotic producing microorganism Immobilization of Enzymes/cells Wine Production and Estimation of alcohol content in the produced wine Production and Estimation of Citric acid from Aspergillus niger Study of Growth curve of microorganisms Study of fermenter operations Precipitation Dialysis Chromatography Thin Layer Chromatography Gel Filtration Chromatography Module 1 - Basics: Biosciences and Computing along with an introduction to PERL programming. Module 2 - Data Collection: Techniques involved in biological data collection and storage in databases. Module 3 - Data Analysis: Analyzing biological data - This includes nucleotide/protein sequences, protein structures etc. Temperature Substrate Concentration Inhibitors Module 4 - Large Scale analysis and Applications: The applications of Bioinformatics and its employment in large scale data analysis.

Serum Proteins Serum Amino Acids DNA Uric acid Free fatty acids

ADVANCED PROFICIENCY COURSES (30 Days) PLANT BIOTECHNOLOGY GENETIC ENGINEERING Instrumentation Reagent preparation Conjugation Transduction Genomic DNA Extraction Plasmid DNA Extraction Agarose Gel Electrophoresis Gel Elution Polymerase Chain Reaction Restriction Digestion Ligation Transformation Total RNA isolation from Blood RT-PCR Gene Expression Affinity Chromatography Ion Exchange Chromatography Gel Filtration Chromatography Western Blotting ELISA DNA Fingerprinting RAPD RFLP Southern Blotting Stock solution, reagent and Media preparation In vitro culturing technique Primary Culture: - Seed, Embryo, Nodal, Internodal, Leaf, Storage organ, Nucellus, Anther, Shoot tip, Root tip, Suspension culture Sub culture: - Callus, Shoot induction, Root induction, Somatic embryo induction Somatic embryo viability test Production of Synthetic seeds Stress tolerance studies and biochemical estimations Protoplast isolation, fusion and culturing Agrobacterium mediated transformation Genomic DNA isolation from plant samples Plasmid DNA isolation from Agrobacterium Screening of transgenic Plants using PCR RAPD and ISSR analysis to analyze the chemically induced Somaclonal variation Secondary metabolite production, purification and confirmation through biochemical tests Histobiochemical GUS assay.

BIOINFORMATICS Introduction and Scope of Bioinformatics The Biological Databases Perl Programming for Bioinformatics Sequence Alignment and Phylogenetic analysis Analyzing Protein and DNA Sequences Structural Bioinformatics and Drug discovery Visualization, Modeling, Dynamics and Docking Genome Analysis Mapping projects and the Human Genome Project Work

INDUSTRIAL BIOTECHNOLOGY Isolation of Industrially important microorganisms from Soil, Water or Air Screening for production of Industrially Important metabolites (Organic Acids, Vitamins, Antibiotics) Strain improvement trials Mass Production of the Metabolite producing organisms Partial purification of the metabolite Assay of the metabolite Characterization of the metabolite Documentation of experiments Report preparation PROTEIN PURIFICATION Introduction to Proteins Structure, Classification and Importance of Proteins Chromatography - Basic principles Types of Chromatography Introduction to Liquid Chromatography system Operation of the LC system Purification of industrially important Proteins using Liquid Chromatography Characterization of the Purified product SDS PAGE Protein Estimation Documentation of experiments Report preparation ENZYME BIOTECHNOLOGY Isolation of Industrially important microorganisms from Soil, Water or Air Screening for production of Enzymes (Amylase, Protease, Cellulase etc.) Strain improvement trials Mass Production of the Enzyme producing organisms Partial purification of the enzymes by any of the methods mentioned below Precipitation techniques Chromatography Characterization of the purified Enzyme SDS PAGE Protein Content Enzyme assay Study of Process Efficiency Enzyme immobilization Assay of the enzyme after immobilization Documentation of experiments Report preparation

PROJECTS

(15-180 Days)

INDUSTRIAL BIOTECHNOLOGY

Industrial Biotechnology is the application of Biotechnology for industrial purposes involving sustainable production of industrially useful products like biochemicals, biomaterials and biofuels from renewable resources, using living cells and/or their enzymes. It also involves large-scale fermentation as is used in some pharmaceutical production. Industrial Biotechnology is mainly based on fermentation technology and biocatalysis. The use of fermenter is not only in production but also in standardization where optimizations of production processes are done to achieve maximum yield since the industrial profit hovers around the criteria of maximum output with minimum input. Immobilization is a break through technology in this arena which allows economical enzyme aided fermentation with reusability of used enzyme and reduced down streaming. Projects offered Isolation, Screening, biochemical characterization, media optimization, Shake flask production, pilot scale production, downstream processing immobilization and enzyme kinetics of any of the enzymes listed: Amylase, Protease, Cellulase, Pectinase, Lipase, Endoglucanase, Invertase etc Production of various industrially important microbial products Enzymes, Vitamins, Antibiotics, Organic acids, Alcohols, Wine, Bacteriocins, Single Cell Proteins etc Production of Various medically important microbial products Mycotoxins, Pigments etc Strain improvement of Industrially important Microorganisms Immobilization studies of various enzymes Large scale production of enzyme, Purification, Quantification, Immobilization, and comparative studies Production and assay of Biofertilizers Rhizobium , Azotobacter, Azospirillum,Cyanobacteria, Liquid Manure etc.

APPLIED MICROBIOLOGY

Applied microbiology is an actively researched multidisciplinary field which is advancing continually. Environmental microbiology involves the study of the composition and physiology of microbial communities in the environment. Medical microbiology deals with the study of pathogens which are of medical importance. Microorganisms are beneficial for biodegradation or bioremediation of domestic, agricultural and industrial wastes and subsurface pollution in soils, sediments and marine environments. Projects offered Effects of heavy metals on microbial growth. Lead, Mercury, Chromium, Cadmium, Arsenic, Copper and Tin etc Bacteriophage Studies Isolation of Bacteriophages, Plaque assay, Purification of phages Invitro Antimicrobial studies of various phytochemical compounds Extraction, antimicrobial assay and purification of compounds Microbiological and Chemical analysis of various compounds (Case studies) Milk, Water, Eatables, Cosmetics, Effluents etc Microbial degradation of environmentally hazardous compound Mycotoxins, dyes, Petroleum compounds, plastics, heavy metals etc

ENZYMOLOGY

Enzymes are biological polymers that catalyze the chemical reactions which make life possible. The presence and maintenance of a complete and balanced set of enzymes is essential for the breakdown of nutrients to supply energy and chemical building blocks. The applications of enzymes are varied and range from medicine to industry. In Medicine for example enzymes like transaminases, lactate dehydrogenases, asparaginases etc are widely studied. In industry, enzymes like amylase, protease, xylanase, catalase etc., play a major role. Enzyme Kinetics is the field of Biochemistry concerned with the quantitative measurement of the rates of enzyme catalysed reactions and the systematic study of factors that affect these rates. The study of enzyme kinetics also represents the principal way to identify potential therapeutic sources that selectively enhance or inhibit the rates of specific enzyme-catalyzed processes.

Projects offered Enzyme characterization and Kinetic studies Study of inhibition kinetics of an enzyme Screening of different organic/inorganic compounds as inhibitors for the enzymes Immobilization of Enzymes/ Cells Comparative study of immobilized and Free enzymes Screening and quantification of different enzymes from single source.

HPLC

The need for high-resolution separation methods for analytical and preparative purposes is growing by the minute in the industries and research areas. HPLC is a stateof-the-art technique that is ideal for such requirements due to its precision, sensitivity and reproducibility. HPLC is widely used in most of the pharmaceutical and Biotech industries. Project Offered Method Development for Antibiotics using HPLC Method Development for Agrochemicals using HPLC

PLANT BIOTECHNOLOGY

Plant Biotechnology is a rapidly expanding discipline. It chiefly involves the introduction of foreign genes into economically important plant species, resulting in crop improvement and the production of novel plants. Plant Biotechnology has many applications in the field of medicine, environment, food, farming and pharmaceutical industries. Projects offered Studies on induction of organogenesis In various plant species Studies on induction of somatic embryos in various plant species Studies on selection and screening of stress tolerant food crops Studies on somaclonal variation in various plant species Production of plant secondary metabolites Agrobacterium mediated gene transformation in plants Mutagenic studies in Plants and confirmation of mutation through DNA fingerprinting. Phytoremediation Cost effective media formulations for Plant Tissue culture. In vitro study of effectiveness of Biofertilizers.
MOLECULAR BIOLOGY

BIOINFORMATICS

Molecular Biology is the study of biological molecules and the molecular basis of structure and function in living organisms. Ever since the revolution in experimental biology occurred, the techniques in molecular biology and genetic engineering have been at the forefront and is still contributing immensely to the research being carried out globally Projects offered Gene Cloning Prokaryotic Genes. Eukaryotic Genes cDNA Cloning TA Cloning Expression, Purification & Detection of proteins Expression studies of cloned Genes DNA Fingerprinting using RAPD Method ISSR Method Identification of SNP (Single Nucleotide Polymorphism) by PCR-RFLP PCR based Molecular Diagnostics Identification of micro organisms using Multiplex PCR Microbial identification by 16s rDNA analysis Mutational studies and molecular characterization of mutants

Bioinformatics is the application of computer technology to the management of biological data. The data for analysis is obtained from techniques like DNA I Protein sequencing, X-Ray Crystallography, Gene expression studies etc. Analysis of such data includes identifying genes, detecting motifs of functional significance, predicting structure from sequence, studying evolutionary relationships etc. It is a rapidly developing branch of biology and is highly interdisciplinary, using techniques and concepts from informatics, statistics, mathematics, chemistry, biochemistry, physics, and linguistics. It has many practical applications in different areas of biology and medicine. Projects offered Homology Modeling and Structure Analysis Sequence Analysis Phylogenetic Analysis

REFRESHER COURSE (Instrumentation Training) (30 days) In this course emphasis is placed both on understanding the different uses of the advanced instruments as well as the principle behind their functioning. This track provides an excellent training experience for life science students and prepares them for careers in industry, higher education or research. Students are provided the rare opportunity to handle advanced instruments like Fermenter, PCR, HPLC, ELISA reader etc as part of this course Course Contents (Practicals) Fermenter Operations Basics of Fermenter Media preparation & sterilization of the fermenter & usage of various probes Pre-culture preparation & inoculation Observing the growth of the cultures in fermenter PCR DNA isolation & Confirmation Amplification of the gene of interest Blotting Techniques Southern Blotting Digesting DNA, Electrophoresis, Transfer of DNA to the membrane & Hybridisation Western Blotting SDS PAGE, Transfer of Protein to the membrane, Immunodetection Gel Documentation Analysis of gels using gel documentation system HPLC History & principle of HPLC Instrumentation & working Chemistry of solvents Software & Result interpretation & solutes Any one analysis ( Pharma compound / Pesticide compound) ELISA Diagnosis of one disease HBsAg / HIV / HCV Use of ELISA reader Calculation of the cut off values Interpretation of the results Spectrophotometer Estimation of absorbance, transmittance, absorption maxima, rate of activity, purity and quantification of biomolecules

Bioinformatics Retrieving of DNA & protein sequences Retrieving protein structures & studying structural features Identifying domains & motifs in proteins Identification of conserved regions in proteins using a multiple sequence alignment. Locating transmembrane regions, signal peptides, chloroplast & mitochondrial transit peptides Plant biotechnology Protoplast Isolation and culturing Production of synthetic seeds Agrobacterium mediated Transformation
CAPSULE COURSES

HPLC Basic Module (3days) History & principle of HPLC Instrumentation & working Software usage & Result Interpretation HPLC - Advanced Module (6 Days) History and Principle Instrumentation and working Software usage Solvent preparation Sample injection Result interpretation Estimation of agrochemicals/pharmaceutical compounds using a preset method PCR: Basic Module (3days) Isolation of Genomic DNA Agarose Gel Electrophoresis Specific Gene Amplification and confirmation PCR: Advanced Module (14 days) Specific Gene Amplification RT-PCR Colony PCR Gradient PCR RAPD PCR Multiplex PCR Nested PCR

Blotting Techniques (5 days) Western Blotting Southern Blotting FPLC (fast Protein Liquid Chromatography(4 days) Usage of Akta Prime Plus Liquid Chromatography System Usage of Akta Primeview Software Gene TransferTechniques (6 days) Transformation Phage titration Conjugation Operation of Fermenter (5 days) Basics of Fermenter Media preparation & insitu sterilization of the fermenter Pre-- culture preparation Inoculation Observing the growth of the cultures in
FOUNDATION COURSES (7 Days)

Screening of Transgenic Plants (3 days) Isolation of Plant Genomic DNA Agarose Gel Electrophoresis Specific Gene Amplification and confirmation of transgene DNA Fingerprinting (6days) RAPD RFLP ISSR Dendrogram Construction ELISA (4days) Use of Microtiter plate reader Diagnosis of Antigens using ELISA reader

Foundation courses are short term introduction courses offered in different departments and disciplines in Biotechnology. They aim at baptizing the student towards Biotechnology and providing them with the basic platform for more advanced research. The training modules compromise individual hands-on training and serve as an introductory exposure to learning highly advanced modules in future. You could choose from the following modules: Industrial Biotechnology Immobilization of Cells Isolation & screening for industrially important bacteria Antibiotic production & testing its efficacy. Aflatoxin production Thin Layer chromatography Quality analysis of milk Immunology Separation of serum & plasma from blood. Erythrocyte Sedimentation Rate (ESR)

Blood smear preparation, Staining & visualization of blood cells Blood counts: Counting of WBC, Platelets, Reticulocytes Estimation of Blood hemoglobin using Haemometer Direct & indirect Antiglobulin Test (Coomb's test) Widal, Preganancy tests Measurement of serum albumin Separation of serum amino acids by paper chromatography RID Molecular Biology Orientation and instrumentation Calculations and Reagent preparation Isolation of Plasmid DNA Isolation of Genomic DNA from Bacteria Total RNA Isolation from Yeast Agarose Gel Electrophoresis Conjugation

Recombinant DNA Technology Orientation and instrumentation Calculations and Chemical preparation Isolation of Plasmid DNA Isolation of Genomic DNA from Bacteria Isolation of total RNA from Yeast. PCR Amplification of DNA Agarose Gel Electrophoresis Restriction Digestion Ligation Plant Biotechnology Stock solution and Chemical Preparation Media Preparation Invitro regeneration techniques Organogenesis Nodal Culture & Leaf Culture Somatic Embryogenesis Suspension Culture Callogenesis Callus Culture Rhizogenesis Invitro rooting Estimation of plant secondary metabolites Estimation of chlorophyll Production of artificial seeds Plant Genomic DNA Isolation Agarose Gel Electrophoresis Clinical Biochemistry Qualitative analysis of urine samples Estimation of glucose from blood and urine Estimation of Level of iron and hemoglobin in blood Lipid profile Test - Estimation of cholesterol, HDL, LDL, Triglycerides, Lactate dehydrogenase Liver function tests - Determination of SGOT and SGPT activity, Albumin/Globulin ratio in serum, Estimation of bilirubin Bioinformatics Bioinformatics History and development and Future challenges.Basics of Linux operating system.

Swiss Prot., KEGG. Sequence comparison by alignment. Types of alignment global and local. Scoring an alignment substitution matrices and gap penalties. BLAST and its applications. Software/Servers : Lalign, BLAST Multiple sequence alignment and Phylogenetic analysis. Software/Servers : Clustal X, ,Phylip. Nucleotide sequence analysis Gene prediction, primer design for PCR. Restriction digestion, Software/Servers : EMBOSS, GENSCAN, ORFFinder, Primer3, NEBWebcutter. Protein sequence analysis studying physico-chemical characteristics, locating signal and transit peptides. Identifying motifs and domains. Software/Servers : ExPaSy, PROSITE, Pfam, Introduction to protein structure Protein structure databases. Software/Servers : PDB, MMDB, CATH, SCOP . Visualizing protein structure data

CONTRACT RESEARCH & CUSTOM SERVICES Genohelix provide contract research services that help pharmaceutical and biotech companies significantly enhance their R&D success. We are committed to exceeding the needs and expectations of our clients by providing innovative, professional and superior quality service, with integrity and reliability. Our services are the best in their class, powered by sophisticated instruments and a well experienced team, providing you with trustworthy solutions to accelerate your research. We offer a complete range of services with personalized attention and flexibility in the following areas: Microbial Identification Services Antimicrobial Activity studies HPLC analysis of Compounds Protein Purification using Liquid Chromatography. PCR optimizations. SNP Analysis Cloning and expression DNA fingerprinting services Extraction and analysis of Phytochemicals Micropropogation of plants Standardization of conditions for Plant tissue culture. Enzyme analysis, Kinetic study and inhibitor studies Optimization of production conditions for different

Jain Group of Institutions

Jain Group of Institutions is a conglomerate of 59 vibrant institutions, corporate divisions, entrepreneurial ventures, distinguished professional alliances and a noteworthy social opportunity model. Initiated by the dynamism of its visionary founder and chairman Dr. R Chenraj Jain, the group is inspired by a shared vision of a self reliant, progressive and a proactive India providing the world leaders of quality and substance. In short span, the group has burgeoned into a benchmark in the education scenario in India.

We provide facilities for M.Phil, Ph.D students & other interested candidates to carry out their research work. Well equipped Molecular Biology, Microbiology, Plant Biotechnology, Industrial Biotechology, Biochemistry labs are available for use. We also conduct onsite customized practical workshops in

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GENOHELIX

GENOHEL X
B I O L A B S

Bus Route : From Majestic City Railway Station City Market 45 A, B, C, D, E, F, G etc., 43 A, B, C, D, E, F etc., 36 A, B, C, D, E, F, etc., 31, 31 E, 35, 10 Alight at : Uma Theatre

GENOHEL X
B I O L A B S
GENOHEL X
B I O L G E N O H E L IA B I O L A B S X B S

127/3, Bull Temple Road, Chamarajpet, Bangalore - 560 019 P : +91 80 2660 4992 / 2660 4993

e-mail : info@genohelix.com www.genohelix.com