Professional Documents
Culture Documents
• Ligase requires ATP and string nucleotides together in nucleic acids and
simple sugars in polysaccharides.
• Lyase breaks bonds between carbon atoms or between carbon and nitrogen.
For example, deaminase removes amino group from amino acid.
• Hydrolase breaks large molecules into simpler molecules with the addition of
a water molecule. For example, proteases split proteins into amino acids;
amylase splits carbohydrates into simple sugars; and lipase breaks apart
triglycerides.
Return to Top
• Food enzymes are found in raw foods. All food, whether animal or plant,
contains enzymes.
• Digestive enzymes are secreted by the body to digest the food eaten. There
are three categories of digestive enzymes:
• Lipases (found in the stomach and pancreatic juices, and in food fats)
aid in fat digestion.
• Metabolic enzymes run all the body organs and systems by performing
various chemical reactions within the body cells. Without them, life would
cease to exist. Two important metabolic enzymes are SOD (superoxide
dismutase, which is an antioxidant and Catalase, which breaks down
hydrogen peroxide, a metabolic waste product, liberating the oxygen for use
in the body.
Return to Top
Digestive enzymes and their function
• Amylase I and II are secreted, first by the salivary glands, and then by the
pancreas. They function best in a pH of 6.7-7.5, breaking bonds between
carbohydrate molecules to produce disaccharides and trisaccharides. Amylase
I is activated by chewing and begins the digestive process by converting
starch (amylose) to maltose. Amylase II, produced by the pancreas, is only
slightly different chemically and continues the process started by the salivary
amylase.
• Lipase is secreted by the pancreas -- but only if bile salts are present. It
functions best in a pH of 7-8. Lipase targets triglycerides to produce fatty
acids and monoglycerides. Lipase also seems to be activated by the presence
of Vitamin C, glutathione, and cysteine.
• Maltase, sucrase, and lactase are secreted by the mucosal cells of the small
intestine and found in the membrane surface of microvilli. They function best
in a pH of 7-8. They respectively target the sugars maltose, sucrose, and
lactose to produce monosaccharides.
• Peptidase is secreted by the mucosal cells of the small intestine and found in
membrane surfaces of the microvilli. It functions best in a pH of 7-8.
Peptidase targets dipeptides and tripeptides to produce amino acids.
Return to Top
• Papain is found in papaya. It is often used with bromelain. Both are used as
meat-tenderizers and to help with protein digestion and in controlling acidic
conditions of the stomach.
• Lactase is obtained from a fungal source Aspergillus niger and a yeast source
Saccharomyces lactis. A. niger is most active in a pH of 4.0 to 5.0, while S.
lactis prefers 6.0 to 8.5. Lactase is used to treat lactose insufficiency and as a
digestive aide.