Chem 7 quizbank 1.

T/F In a classic competitive heterogeneous RIA to measure t4 as the concentration of analyte in the test sample increases the concentration of bound-labeled molecule decreases (true) 2. The strength of binding between an antigen and antibody is related to the: goodness of fit between the epitope and the F(ab) 3. Which homogeneous immunoassay relies on inhibiting the activity of the enzyme label when bound to antibody reagent to eliminate separating free from bound-labeled reagent? [EMIT] 4. Which unlabeled immune precipitation method in gel is used to quantitate a serum protein? [radial Immunodiffusion] 5. In flow cytometry the side scatter is related to the [Granularity of the cell] 6. True or false in a classis competitive heterogeneous RIA o measure t4 the tracer is t4 with a radiolabel [True] 7. In monoclonal antibody production the specificity of the antibody is determined by the [sensitized b lymphocytes] 8. In Immunofixation electrophoresis discrete bands appear at the same electrophoretic location, one reacted with antihuman IgA ( alpha chain specific) reagent and the other reacted with antihuman reagent [An IgA monoclonal protein] 9. Which unlabeled immune precipitation method in gel is used to quantitate a serum protein? [Radial Immunodiffusion] 10. You analyze the DNA content on a sample breast tissue for suspected malignancy using flow cytometry and get the following results DI=2.5 and % cells in S phase =29% . based on these results you can conclude that [These results are likely indicative of a malignant breast tumor] 11. Match terms a. Proteins are the target western blot b. Messenger RNA is the target northern blot c. Single stranded DNA is the target southern blot 12. In nephelometry the antigen antibody complex formation is enhanced in the presence of: [Polyethylene glycol] 13. T/F in a classic competitive heterogeneous RIA t measure t4 the assay requires a separation step [True] 14. t/f in classic competitive heterogeneous RIA to measure t4 as the concentration of analyte in the test sample increases the concentration of bound labeled molecule decreases [True] 15. number the steps of western blot a. protein antigen are isolated and denatured b. proteins are separated by SDS PAGE c. proteins are transferred to a nitrocellulose membrane

Chem 7 quizbank d. test serum is incubated with the proteins e. labeled antiserum reagent that reacted is evaluated 16. true false in the classic competitive heterogeneous RIA to measure the t4 the detector molecule is radiolabeled anti t4 [False] 17. the nucleic acid technique in which RNA is transferred to DNA which is then amplified is known as [RT-PCR]