PROTIOMECs

proteomics
Blocking Reagents ..................................... 212 - 213 Chemiluminescent Substrates...................218 - 219 Chromatography Buffers ...................................206 Detergents ...........................................................205 Electrophoresis Products ...........................208 - 210 Introduction to ProPure Products ..................196 Labeling Reagents .............................................. 215 ProPure Products ....................................197 - 203 Protease Inhibitors..............................................204 Protein Assays .....................................................207 Protein Transfer................................................... 211 Stripping Buffers.................................................220 Wash Buffers ....................................................... 214 Western Blotting Kits ................................ 216 - 217
Pro-Pure Products
High Purity, Stringently Tested Products for Proteomics Research Free of Protease Activity Functionally Tested Where Applicable
The study of the proteome, or Proteomics, relies on analytical means to identify and quantify proteins in their various forms. It is an extensive field that encompasses techniques in fields such as: Protein Expression and Purification, Protein Separation, Protein Visualization, and Protein Identification and Characterization. As proteins are highly sensitive to a variety of chemicals that can destroy their activity and/or degrade their structure, proteomics grade materials must be used to ensure the integrity and quality of the sample. AMRESCOs Pro-Pure line provides products that are of the highest purity and offer extreme sensitivity. This product line is stringently manufactured and tested to ensure optimal results in proteomics research. By using more sensitive tools, more precise interpretation of the results is possible. Protein Expression and Purification AMRESCOs affinity chromatography buffers are designed for optimal purification and recovery of recombinant proteins. Refer to page 206 of this section. Protein Separation NEXT GEL ready-to-pour acrylamide solutions produce high sensitivity acrylamide gels and enable a high resolution visual comparison between the separated proteins. For a complete listing of all NEXT GEL products as well as AMRESCOs other electrophoresis products, refer to pages 153 - 158 of the Electrophoresis section of this catalog.
Protein Visualization AMRESCO offers a wide range of products for the visualization of proteins both in a gel and membrane bound. For a complete listing and a description of these products, refer to pages 166 - 171 of the Electrophoresis section of this catalog.
Protein Identification AMRESCO offers a wide range of products for the identification of individual proteins. Refer to pages 216 - 220 of this section.
196 PROTEOMICS
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Acr - Bra
Pro-Pure Products
ACRYLAMIDE
Product code Size Price
BENZAMIDINE HYDROCHLORIDE
M120
100 g 500 g 1 kg
$39.50 $131.30 $207.20
M141
25 g 50 g 100 g
$42.10 $72.00 $115.60
Proteomics, 204
ACRYL-40
A 40% (W/V) Solution of Ultra Pure Acrylamide. Product code Size Price
BES FREE ACID

M121
100 ml 500 ml
$12.30 $45.40
M225
Electrophoresis, 162
100 g 500 g
$67.40 $244.90
ACRYL/BIS 37.5:1
40% (W/V) SoLutioN A 40% (w/v) solution of Ultra Pure Acrylamide (38.96%) and bis-Acrylamide (1.04%) at a final ratio of 37.5:1. Product code Size Price
BESTATIN
M179
Proteomics, 204
10 mg
$125.80
M157
100 ml 500 ml
$17.00 $59.90
ACRYL/BIS 37.5:1
PreMiXed PoWder Ultra Pure Acrylamide and bis-Acrylamide powders blended to achieve a final ratio of 37.5:1. Reconstituted with deionized water, 40 g size has a final volume of 100 ml; 200 g size has a final volume of 500 ml. Reconstitution will produce a 40% (w/v) solution. Product code Size Price
BIS-ACRYLAMIDE
M104
50 g 100 g 250 g
$28.20 $52.10 $103.20
M156
40 g 200 g
$28.40 $92.40
BIS-2
2% SoLutioN oF BiS-AcrYLAMide Product code Size Price
AEBSF
(4-(2-AMiNoetHYL)-BeNzeNeSuLFoNYL FLuoride, HYdrocHLoride) Product code Size Price
M193
500 ml
$24.40
BIURET PROTEIN ASSAY REAGENT

Sufficient reagent to run 50-70 samples. Product code Size Price
M182
50 mg 250 mg 500 mg
$45.40 $138.40 $268.70
Proteomics, 204
M262
Proteomics, 207
500 ml
$35.70
AMMONIUM PERSULFATE
(APS) Product code Size Price
BLUE BANDIT PROTEIN STAIN

Sufficient reagent to run 50 mini gels. Product code Size Price
M133
25 g 100 g
$11.20 $26.20
M192
1L
$69.40
AMMONIUM SULFATE
BORIC ACID
M105
1 kg 5 kg
$22.30 $71.40
M139
ANTIPAIN DIHYDROCHLORIDE
500 g 1 kg 2.5 kg
$21.50 $36.50 $65.00
M181
Proteomics, 204
5 mg
$41.30
BRADFORD REAGENT
M172
Proteomics, 207
Size Price
1L
$108.40
APROTININ
Product code
M167
Proteomics, 204
10 mg 50 mg
$62.90 $257.60
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PROTEOMICS 197
Pro-Pure Products
Bra - Gly
BRADFORD METHOD PROTEIN ASSAY KIT

The Kit contains 1 L of Bradford Reagent, 200 ml of 0.15 N Sodium Chloride, and 1.5 ml of 0.5 mg/ml BSA. Product code Size Price
COOMASSIE BRILLIANT BLUE R-250

M128
M173
Proteomics, 207
1 Kit
$110.40
10 g 25 g 50 g
$22.80 $43.50 $66.70
DANSYL CHLORIDE BRIj 35, 10% SOLUTION

PeroXide-Free deterGeNt iN SiNGLe-uSe 10ML AMPouLeS Product code Size Price Product code Size Price
M125
5g 10 g
$77.00 $135.50
M238
5 Pk
$55.20
Proteomics, 205, Buffers & Detergents, 230
DETERGENT SAMPLER, PEROXIDE FREE

Contains a 10 ml ampoule of each of the following: Brij 35 10% solution, Tween 20 10% solution, Triton X-100 10% solution and Triton X-114 10% solution. Product code Price Size Price
BRIj 35
(PoLYoXYetHYLeNe LAurYL etHer) Product code Size
M248
4 pk
$47.70
M106
Proteomics, 205
1 kg 5 kg
$40.00 $139.20
Proteomics, 205 , Buffers & Detergents, 230
DL-DITHIOTHREITOL
(dtt) Product code Price Size Price
BROMOPHENOL BLUE SODIUM SALT

Product code Size
M109
M116
50 g 100 g
$53.10 $100.70
5g 25 g
$69.30 $236.30
E-64
(trans-ePoXYSucciNYL-L-LeucYLAMido(4-GuANidiNo)ButANe) Product code Size Price
CHAPS
(3-[(3-cHoLAMidoProPYL)diMetHYL-AMMoNio]-1-ProPANeSuLFoNAte) Product code Size Price
M186
Proteomics, 204
5 mg
$85.40
M127
5g 10 g 50 g
$63.30 $105.50 $420.00
Proteomics, 205
EDTA DISODIUM SALT DIHYDRATE

CHAPSO
(3-[(3-cHoLAMidoProPYL) diMetHYLAMMoNio]-2-HYdroXY-1ProPANeSuLFoNAte) Product code Size Price
M101
500 g 1 kg 2.5 kg
$49.30 $75.30 $165.50
Proteomics, 204
M165
1g 5g 10 g
$43.50 $160.60 $268.30
FORMALDEHYDE, 37% SOLUTION

Proteomics, 205
M134
200 ml 500 ml
$19.70 $25.20
CHYMOSTATIN
GLUTARALDEHYDE, 50% SOLUTION

M184
Proteomics, 204
5 mg
$71.40
M155
100 ml 500 ml
$22.60 $61.00
a-CHYMOTRYPSIN
GLYCEROL
M102
1g 5g
$52.00 $228.70
M152
1L 4L
$26.00 $113.70
COOMASSIE BRILLIANT BLUE G-250

GLYCEROL, STERILE
M140
10 g 25 g 50 g
$22.20 $34.30 $61.30
M153
50 ml 100 ml
$13.75 $21.60
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Gly - Mop
Pro-Pure Products
GLYCEROL, 20% STERILE SOLUTION

IMIDAZOLE CHROMATOGRAPHY BUFFER, 5X

$18.50
pH 7.0 eLutioN BuFFer For NicKeL AFFiNitY coLuMNS Product code Size Price
M154
100 ml
GLYCINE
M264
Proteomics, 206
100 ml 500 ml
$38.50 $191.80
M103
1 kg 5 kg
$32.50 $141.80
IODOACETAMIDE
GLYCINE CHROMATOGRAPHY BUFFER, 5X

pH 3.0 eLutioN BuFFer For ProteiN A ANd ProteiN G AFFiNtY coLuMNS Product code Size Price
M216
30 g
$73.50
IODOACETIC ACID SODIUM SALT

M265
Proteomics, 206
100 ml 500 ml
$27.40 $89.30
M119
10 g 50 g
$58.00 $260.00
GUANIDINE HYDROCHLORIDE
LEUPEPTIN
M110
100 g 250 g 500 g
$35.10 $62.50 $107.80
M180
Proteomics, 204
5 mg 25 mg
$44.00 $133.90
GUANIDINE HYDROCHLORIDE, 8M SOLUTION

LP-NEXT GEL ELECTROPHORESIS KIT

HorizoNtAL AGAroSe GeL eLectroPHoreSiS Kit For LArGe ProteiNS Each kit contains NEXT GEL Sample Buffer, 4X, NEXT GEL Running Buffer, 20X and Agarose HRP. There are sufficient reagents to perform 35-50 mini gels. Product code Size Price
M113
4L
$736.70
HTS NEXT GEL KIT

HiGH tHrouGHPut eLectroPHoreSiS oF ProteiNS. Each kit containes NEXT GEL sample loading buffer, NEXT GEL Running Buffer, and HTS Agarose. There are sufficient reagents to run five 25cm x 25cm gels. Product code Size Price
M272
Electrophoresis, 157, Proteomics, 210
1 Kit
$112.20
L-LYSINE MONOHYDRATE
M281
Proteomics, 210, Electrophoresis, 158
1 Kit
$291.50
M234
100 g 500 g
$109.50 $263.00
IEF ANODE BUFFER, 20X

2-MERCAPTOETHANOL
( b-MercAPtoetHANoL) Product code Size Price
M218
250 ml 4 X 250 ml
$11.70 $35.00
M131
100 ml 250 ml
$13.40 $18.40
IEF CATHODE BUFFER, 20X

MOPS FREE ACID

M243
250 ml 4 X 250 ml
$55.00 $172.60
M214
100 g
$52.00
IMIDAZOLE
MOPS SODIUM SALT

M136
10 g 50 g 100 g
$32.30 $105.00 $125.00
M215
100 g
$45.50
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PROTEOMICS 199
Pro-Pure Products
Nat - Pep
NATIVE NEXT GEL ELECTROPHORESIS KIT

NoN-deNAturiNG GeL eLectroPHoreSiS Kit Each kit contains Native NEXT GEL sample buffer, 4X, Native NEXT GEL Running Buffer 20X, and Agarose HRP. There are sufficient reagents to perform 35-50 mini gels. Product code Size Price
NON-FAT POWDERED MILK

Sufficient material per pack to prepare 1L of a 1X solution. Product code Size Price
M203
10 Pk
$18.40
M271
1 Kit
$95.40
NONIDET P-40 SUBSTITUTE

(octYLPHeNoXYPoLYetHoXYetHANoL) Product code Size Price
NEXT GEL, 5% SOLUTION

Pre-MiXed, reAdY-to-Pour AcrYLAMide SoLutioN For tHe SdS-PAGe ANALYSiS oF ProteiNS Product code Size Price
M158
50 ml 100 ml 500 ml
$14.10 $21.50 $68.80
M254
100 ml 500 ml
$30.80 $104.50
N-OCTYL-b-D-GLUCOPYRANOSIDE
NEXT GEL, 7.5% SOLUTION

M129
500 mg 1g 5g
$25.50 $40.30 $159.00
Proteomics, 205
M255
100 ml 500 ml
$30.80 $104.50
OCTYL-b-D-THIOGLUCOPYRANOSIDE

M130
Proteomics, 205
100 mg 1g
$15.90 $36.80
M256
100 ml 500 ml
$30.80 $104.50
PBS WITH BSA

PoWder BLocKiNG AGeNt Sufficient material per pack to prepare 1L of a 1X solution. Product code Size Price
NEXT GEL, 12.5% SOLUTION

M233
Proteomics, 213
5 Pk
$55.20
PBS WITH NON-FAT POWDERED MILK

M257
100 ml 500 ml
$30.80 $104.50
M232
5 Pk
$30.00

Proteomics, 213
PBS WITH TWEEN 20

M258
100 ml 500 ml
$30.80 $104.50
M245
Proteomics, 213
Price
5 Pk
$20.30
NEXT GEL SAMPLE LOADING BUFFER, 4X

Product code Size
PEPSIN 1:3000
PorciNe Source Product code Size Price
M260
5 ml
$15.90
NEXT GEL TRANSFER BUFFER

M142 $78.80
250 g
$43.10
M279
Proteomics, 211
500 ml
PEPSTATIN
M183
Proteomics, 204
5 mg 25 mg
$39.00 $102.60
200 PROTEOMICS
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Phe - Sod
Pro-Pure Products
PHENYLMETHYLSUFONYL FLUORIDE
(PMSF) Product code Size Price
PROTEASE INHIBITOR COCKTAIL, PLANT

M145
Proteomics, 204
5g 25 g
$34.00 $113.80
M307
Proteomics, 204
1 ml
$35.00
PROTEIN GEL LOADING BUFFER, 2X

WitH PYroNiN Product code Price Size Price
1,10-PHENANTHROLINE MONOHYDRATE
(o-Phenanthroline) Product code Size
M266
5 ml
$12.00
M135
Proteomics, 204
10 g 50 g
$37.10 $137.80
PROTEIN STAIN, IN GEL

StAiNS ProteiNS duriNG eLectroPHoreSiS Product code Size Price
PHOSPHATE CHROMATOGRAPHY BUFFER, 5X

pH 7.4 AFFiNtY coLuMN BiNdiNG BuFFer Product code Size Price
M252
100 ml 1L
$21.80 $73.50
M263
Proteomics, 206
100 ml 500 ml
$27.40 $89.30
SEPARATING GEL BUFFER, 4X

PHOSPHORAMIDON
M197
500 ml
$47.70
SILVER BULLIT SILVER STAIN KIT

Sufficient reagents to stain 50 mini gels. Product code Size Price
M185
Proteomics, 204
5 mg
$134.90
M227
1 Kit
$139.20
PROACT MEMBRANE STAIN

A fast stain for the detection of proteins on nitrocellulose or PVDF membranes. Used to confirm transfer in Western blotting procedures. Product code Size Price
SILVER NITRATE
M282
1L
$81.10
M122
25 g 100 g 500 g
$57.20 $178.10 $532.20
PROTEASE INHIBITOR COCKTAIL, BACTERIAL

SODIUM CARBONATE ANHYDROUS

M306
Proteomics, 204
5 ml
$38.20
M138
500 g 1 kg
$20.40 $39.90
PROTEASE INHIBITOR COCKTAIL, GENERAL USE

SODIUM DODECYL SULFATE

(SdS) Product code Size Price
M221
Proteomics, 204
1 ml
$35.00
M107
PROTEASE INHIBITOR COCKTAIL, GENERAL USE, WITH EDTA

100 g 250 g 500 g 1 kg
$30.50 $67.80 $105.00 $165.00
Proteomics, 205
M222
Proteomics, 204
1 ml
$35.00
SODIUM DODECYL SULFATE, 10% SOLUTION

(SdS) (W/V) SoLutioN Product code Size Price
PROTEASE INHIBITOR COCKTAIL, MAMMALIAN

M250
Proteomics, 204
1 ml
$35.00
M111
Proteomics, 205
100 ml
$20.10
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PROTEOMICS 201
Pro-Pure Products
Sod - Tri
SODIUM DODECYL SULFATE, 20% SOLUTION

(SdS) (W/V) SoLutioN Product code Size Price
TEMED
(N,N,N',N'-tetrAMetHYLetHYLeNe-diAMiNe) Product code Size Price
M112
Proteomics, 205
200 ml 500 ml
$22.50 $44.00
M146
25 ml 50 ml 100 ml
$17.10 $28.60 $49.10
TG BUFFER, 10X SODIUM HYDROXIDE PELLETS

Product code Size Price triS-GLYciNe 10X LiQuid coNceNtrAte A 1X working solution contains 0.025 M Tris base, 0.192 M Glycine. Product code Size Price
M137
500 g 1 kg
$24.60 $36.50
M114
1L 4L
$19.30 $68.20
SODIUM THIOSULFATE PENTAHYDRATE

TG BUFFER
PoWder ANd 10X reAdY-PAcK Each pack makes 1L of 10X concentrate. 40L size contains sufficient dry powder to prepare 40L of 1X Buffer. 1X TG solution contains 0.025 M Tris base, 0.192 M Glycine. Product code Size Price
M132
500 g
$15.10
STACKING GEL BUFFER, 4X

M115
M198
500 ml
$47.70
40 L 2 Pk
$31.80 $34.50
SUCROSE
rNASe ANd dNASe Free Product code Size Price
TG-SDS BUFFER, 10X

10X LiQuid coNceNtrAte (triS-GLYciNe-SodiuM dodecYL SuLFAte) A single strength (1X) solution contains 0.025 M Tris base, 0.192 M Glycine, and 0.1% Sodium Dodecyl Sulfate. In the 4L pack size, there is a sufficient concentrate to prepare 40 L of 1X TG-SDS Buffer. Product code Size Price
M117
500 g 1 kg
$17.30 $29.40
SUCROSE, 20% STERILE SOLUTION

M148
1L 4L
$27.30 $76.30
M118
100 ml
$36.20
TG-SDS BUFFER
PoWder ANd 10X reAdY-PAcK Each pack makes 1 L of 10X concentrate. 40 L size contains sufficient dry powder to prepare 40 L of 1X Buffer. 1X solution contains, 0.025 M Tris, 0.192 M Glycine, and 0.1% SDS. Product code Size Price
TBS WITH BSA

PoWder BLocKiNG AGeNt Sufficient material per pack to prepare a 1L of a 1X solution. Product code Size Price
M149
M231
Proteomics, 213
5 Pk
$60.70
40 L 2 Pk
$36.20 $36.80
THIOUREA
TBS WITH NON-FAT POWDERED MILK

M226
100 g 500 g
$45.00 $67.00
M230
Proteomics, 213
5 Pk
$32.00
TRACKING DYE - 2D
M217
1 ml
$13.20
TBS WITH TWEEN 20

TRICINE BUFFER
(N-triS(HYdroXYMetHYL)MetHYLGLYciNe) Product code Size Price
M235
Proteomics, 213
5 Pk
$19.90
M159
100 g 500 g
$28.60 $93.40
202 PROTEOMICS
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Tri - Zip
Pro-Pure Products
TRIS, FREE BASE

(triS(HYdroXYMetHYL)AMiNoMetHANe) Product code Size Price
TRITON X-114
M151
500 g 1 kg
$36.50 $65.00
M144
Proteomics, 205
1L 4L
$23.50 $76.80
TRIS HYDROCHLORIDE
TRYPSIN
M108
500 g 1 kg
$63.60 $85.40
M150
1g 5g
$42.40 $154.20
TRYPSIN INHIBITOR, SOYBEAN

TRIS-TRICINE BUFFER, 10X

10X LiQuid coNceNtrAte A 1X solution contains 100 mM Tris and 100 mM Tricine. Product code Size Price
M191
Proteomics, 204
1g 10 g
$62.40 $442.90
M170
1L
$89.50
TWEEN 20, 10% SOLUTION

PeroXide-Free deterGeNt iN SiNGLe-uSe 10ML AMPouLeS Product code Size Price
TRIS-TRICINE BUFFER, 10X

10X reAdY-PAcK A 1X solution contains 100 mM Tris and 100 mM Tricine. Sufficient material per pack to prepare 1L of a 10X solution. Product code Size Price
M228
5 Pk
$47.70
M171
1 Pk
$79.50
TWEEN 20
(PoLYoXYetHYLeNe-20-SorBitAN MoNoLAurAte) Product code Size Price
TRIS-TRICINE SDS BUFFER, 10X

10X LiQuid coNceNtrAte A 1X SoLutioN coNtAiNS 100 MM triS, 100 MM triciNe ANd 3.5 MM SdS. Product code Size Price
M147
50 ml 1L 4L
$11.50 $26.40 $64.10
M168
1L
$89.50
Proteomics, 205, Buffers & Detergents, 228-229
TRIS-TRICINE SDS BUFFER, 10X

10X reAdY-PAcK A 1X SoLutioN coNtAiNS 100 MM triS, 100 MM triciNe ANd 3.5 MM SdS. Sufficient material per pack to prepare 1L of a 10X solution. Product code Size Price
TWEEN 80
(PoLYoXYetHYLeNe-20-SorBitAN MoNooLeAte) Product code Size Price
M126
M169
1 Pk
$86.00
100 ml 1L 4L
$15.60 $26.40 $52.90
Proteomics, 205, Buffers & Detergents, 228-229
Triton X-100, 10% Solution

PeroXide-Free deterGeNt iN SiNGLe-uSe 10ML AMPouLeS Product code Size Price
UREA
M236
5 Pk
$55.20
M123
100 g 500 g 1 kg 2.5 kg
$18.50 $32.20 $48.00 $95.40
TRITON X-100
t-octYLPHeNoXYPoLYetHoXYetHANoL Product code Size Price
UREA, 8M SOLUTION
M143
Proteomics, 205
1L 4L
$22.40 $72.20
M124
250 ml
$42.13
ZIP REVERSIBLE PROTEIN DETECTION KIT

Sufficient reagents to stain 25-50 mini gels. Product code Price Size Price
TRITON X-114, 10% SOLUTION

PeroXide-Free deterGeNt iN SiNGLe-uSe 10ML AMPouLeS Product code Size
M277
1 Kit
$94.90
M237
5 Pk
$52.50
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PROTEOMICS 203
Protease Inhibitors
Protein Protection
Protease InhIbItors
Extensive Offering Inhibits a Wide Range of Proteases Effectively Preserve the Native State of Proteins Protease Inhibitor Cocktails are Convenient & Easy-to-use
Powerful proteases can begin degrading your protein samples from the moment cells or tissues are lysed. Protein yield and sample quality can be drastically reduced by proteases. Complex protein solutions are usually treated with a mixture of several protease inhibitors in order to preserve the native state of proteins.
Product deScriPtioN Protease inhibitors SPeciFitY oF iNHiBitioN An irreversible serine protease inhibitor of chymotrypsin, trypsin, kallikrein, plasmin and thrombin. Useful as a non-toxic alternative for PMSF. A reversible cysteine and serine protease inhibitor of trypsin, papain and cathepsin A and B. Slightly inhibits plasmin. A competitive and reversible serine protease inhibitor of chymotrypsin, trypsin, kallikrein and plasmin. Not an inhibitor of Factor Xa and thrombin. A protease inhibitor of arginine-selective enzymes like trypsin, kallikrein and thrombin. A competitive inhibitor of aminopeptidases, especially aminopeptidase B, leucine aminopeptidase and tripeptide aminopeptidase. Not an inhibitor of carboxypeptidases. A reversible serine protease inhibitor of a, b, g, d-chymotrypsin. Also inhibits papain and many other cysteine proteases. An irreversible inhibitor of cysteine proteases like papain, calpain and cathepsin B, H, L and S. Also an effective inhibitor of collegenase. A reversible metalloprotease inhibitor useful for chelating metal ions. A reversible inhibitor of cysteine proteases and serine proteases resembling trypsin. Inhibits trypsin, plasmin, papain, kallikrein, thrombin and cathepsin A and B. An aspartic acid protease inhibitor of pepsin, renin and cathepsin D. Also an inhibitor of the HIV protease. Not water soluble. An irreversible serine protease inhibitor of trypsin, chymotrypsin, kallikrein, subtilisin and thrombin. Also inhibits the cysteine protease papain. A metalloprotease inhibitor useful for chelating iron and other divalent metals. A specific inhibitor of thermolysin and other metalloproteases. Also an effective inhibitor of collegenase. Contains a mix of protease inhibitors: AEBSF, Aprotinin, E-64, Bestatin and Leupeptin Contains a mix of protease inhibitors: AEBSF, Aprotinin, E-64, Bestatin and Leupeptin Contains a mix of protease inhibitors: AEBSF, E-64, Bestain, Leupeptin and EDTA Disodium Dihydrate Contains protease inhibitors for mammalian cell use: AEBSF, Aprotinin, E-64, Bestatin, Leupeptin and Pepstatin Contains a mix of protease inhibitors: AEBSF, E-64, Bestain, Pepstatin, Leupeptin and 1, 10-Phenanthroline A serine protease inhibitor specific for trypsin and trypsin-like proteases. WorKiNG rANGe 1.1 1.0 mg/ml (0.4 4 mM) 50 g/ml (74 M) 0.06 2.0 g/ml (0.01 0.3 M) 0.1 0.6 g/ml (0.5 4 mM) 40 g/ml (130 M) 6 60 g/ml (10 100 M) 0.5 10 g/ml (0.14 28.0 M) 1.2 0.5 mg/ml (0.5 1.3 mM) 0.5 g/ml (1 M) 0.7 g/ml (1 M) 17 170 g/ml (0.1 1.0 mM) 4 g/ml (20 mM) 4 330 g/ml (7 570 M) 1X 1X 1X 1X 1X Use at equimolar concentrations code M182-50MG M182-250MG M182-500MG M181-5MG M167-10MG M167-50MG M141-25G M141-50G M141-100G M179-10MG Size 50 mg 250 mg 500 mg 5 mg 10 mg 50 mg 25 g 50 g 100 g 10 mg See PAGe 197
AEBSF* Antipain Dihydrochloride* Aprotinin* Benzamidine Hydrochloride* Bestatin* Chymostatin* E-64* EDTA Disodium Salt* Dihydrate Leupeptin* Pepstatin* Phenylmethylsulfonyl Fluoride (PMSF) 1, 10-Phenanthroline Monohydrate* Phosphoramidon
Protease inhibitor cocktails
197 197
197
197
M184-5MG M186-5MG M101-500G M101-1KG M101-2.5KG M180-5MG M180-25MG M183-5MG M183-25MG M145-5G M145-25G M135-10G M135-50G M185-5MG
5 mg 5 mg 500 g 1 kg 2.5 kg 5 mg 25 mg 5 mg 25 mg 5g 25 g 10 g 50 g 5 mg
198 198 198
199
200 201
201
201
Protease Inhibitor Cocktail, 100X Protease Inhibitor Cocktail, 100X with EDTA Protease Inhibitor Cocktail, Bacterial 100X Protease Inhibitor Cocktail, Mammalian, 100X Protease Inhibitor Cocktail, Plant 100X Trypsin Inhibitor, Soybean
* Product is Animal-free
M221-1ML M222-1ML M306-5ML M250-1ML M307-1ML M191-1G M191-10G
1 ml 1 ml 5 ml 1 ml 1 ml 1g 10 g
201 201 201 201 201 203
204 PROTEOMICS
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Detergents
Purification & Isolation
PeroxIde Free detergents

Optimized for Isolating Proteins & Sample Preparation Contains < 1 g of Peroxide Per ml Provided in Ready-to-use Concentrations, Pre-measured in Ampoules Offers Convenient & Long Term Stability
AMRESCOs high purity Pro-Pure grade detergents are carefully packed under inert gas, making them extremely stable. The detergents are provided in ready-to-use 10% solutions that are packaged in 10 ml ampoules for optimal convenience.
Product deScriPtioN
Brij 35, 10% Solution Triton X-100, 10% Solution Triton X-114, 10% Solution Tween 20, 10% Solution Detergent Sampler Pack
code M238-10ML-5PK M236-10ML-5PK M237-10ML-5PK M228-10ML-5PK M248-4PK
Size 5 pack 5 pack 5 pack 5 pack 4 pack
See PAGe 198 203 203 203 198
Contains one 10 ml ampoule of each of the following: Brij 35, 10% Solution Triton X-100, 10% Solution Triton X-114, 10% Solution Tween 20, 10% Solution
standard detergents For ProteomIcs Work

Highly Purified and Suitable for Most Biological Applications Protease-free
Product deScriPtioN code
M106-1KG M106-5KG M127-5G M127-10G M127-50G M165-1G M165-5G M165-10G M158-50ML M158-100ML M158-500ML M129-500MG M129-1G M129-5G M130-100MG M130-1G M107-100G M107-250G M107-500G M107-1KG M111-100ML M112-200ML M112-500ML M143-1L M143-4L M144-1L M144-4L M147-50ML M147-1L M147-4L N126-100ML N126-1L N126-4L
Size
1 kg 5 kg 5g 10 g 50 g 1g 5g 10 g 50 ml 100 ml 500 ml 500 mg 1g 5g 100 mg 1g 100 g 250 g 500 g 1 kg 100 ml 200 ml 500 ml 1L 4L 1L 4L 100 ml 1L 4L 100 ml 1L 4L
See PAGe
198 198
Brij-35 CHAPS CHAPSO Nonidet P-40 Substitute N-Octyl-b-D-Glucopyranoside Octyl-b-D-Thioglucopyranoside Sodium Dodecyl Sulfate (SDS)
198
200
200
200 201
Sodium Dodecyl Sulfate (SDS), 10% (w/v) Solution Sodium Dodecyl Sulfate (SDS), 20% (w/v) Solution Triton X-100 Triton X-114 Tween 20 Tween 80
201 202 203 203 203
203
Please refer to the Buffers & Detergents section pages 208 - 209 for more information on Detergents
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PROTEOMICS 205
Purification & Isolation
Chromatography
Related Products
Ammonium Acetate Ammonium Phosphate Monobasic Glutathione PBS Potassium Phosphate tris
aFFInIty column chromatograPhy buFFers

Liquid Concentrate, Ready-to-use Proteomics Grade Buffers for Protein Isolation Convenient For Everyday Use as Well as in High Throughput Expression, Purification & Characterization of Recombinant Proteins Consistent Performance from Lot-to-lot Easy-to-use
AMRESCO offers three affinity column chromatography buffers to optimize the purification and recovery of recombinant proteins. The Phosphate Chromatography Buffer is an affinity column binding buffer used in metal chelate chromatography. Two types of elution buffers are available, designed specifically for use with His-Tag fusion proteins and monoclonal antibodies. The Imidazole Chromatography Buffer is an elution buffer for nickel affinity column chromatography used to isolate His-Tag fusion proteins. The Glycine Chromatography Buffer is an elution buffer for Protein A and Protein G affinity columns, used for isolating monoclonal antibodies.
FuSioN tAG
iMMoBiLized LiGANd
GeNerAL BiNdiNG coNditioNS
GeNerAL eLutiNG coNditioNS
eLutioN BuFFer
Polylhistidine (6X His) Monoclonal antibody
Chelated Ni2+ or Co2+ Protein A, Proten G sepharose
Neutral, pH without redox agents. Tag must be accessible in protein structure. High ionic strength, neutral pH
coNceNtrAtioN 5X 5X 5X pH 7.4 7.0 3.0
> 200 mM Imidazole, low pH Low pH, decrease in ionic strength

code M263-100ML M263-500ML M264-100ML M264-500ML M265-100ML M265-500ML Size
Imidazole Buffer (Code: M264) Glycine Buffer (Code: M265)

See PAGe 201 199 199
Product deScriPtioN
Phosphate Chromatography Buffer, Binding Buffer Imidazole Chromatography Buffer, Elution Buffer (His-Tag) Glycine Chromatography Buffer, Elution Buffer (GST Tag)
100 ml 500ml 100 ml 500ml 100 ml 500ml
hPlc grade solvents

High Purity Functionally Tested Reagents Suitable for HPLC and Mass Spectrometry Low UV Absorbing & Low Particulate Count to Reduce Background
Product deScriPtioN code K981 -1L K981-4L N170-4L N169-4L K977-1L K977-4L N167-4L N168-4L K978-1L K978-4L Size 1L 4L 4L 4L 1L 4L 4L 4L 1L 4L See PAGe 13 13 14 78 78 78 133
Acetonitrile Acetonitrile with 0.1% Glacial Acetic Acid Acetonitrile with 0.1% Trifluoroacetic Acid Methanol Methanol with 0.1% Glacial Acetic Acid Methanol with 0.1% Trifluoroacetic Acid Water, Sterile
206 PROTEOMICS
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Protein Assays
Protein Detection
bradFord ProteIn assay kIt

Fast, Consistent & Sensitive Protein Assay Kit Determine Protein Concentrations from 1 100 g Fast Color Change is Read at 595 nm
The Bradford Protein Assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie Brilliant Blue G-250 dye1, the Bradford method is easier, faster and more sensitive than the Lowry method. The standard protocol is useful for measuring between 10 - 100 g of protein and the microassay is useful for measuring between 1 - 10 g of protein. For maximum convenience, Bradford Reagent and 0.5 mg/ml BSA standard solution are offered separately or together as a kit.
1. Bradford, M. (1976) Anal. Biochem., 72, 248-254.
Related Products
NeXt GeL Products Protein Molecular Weight Markers Protein Stain, in-Gel Blue BANdit Protein Stain Silver BuLLit Staining Kit BSA Standards Kit
Product deScriPtioN
code M173-1KIT
Size 1 Kit
See PAGe 198 Biuret Protein Assay
Bradford Protein Assay Kit
Contains: Bradford Reagent, 1 L Albumin, Bovine - 0.5 mg/ml Standard, 1.5 ml
Bradford Reagent Albumin, Bovine - 0.5 mg/ml Standard
M172-1L E531-1.5ML
1L 1.5 ml
197 20
bIuret ProteIn assay reagent

Determine Protein Concentrations from 1 10 mg/ml Very Few Interfering Agents Color Change is Read at 550 nm Ready-to-use Reagent is Stable For at Least Two Years
Biuret Assay Standard curve

A solution of Bovine Serum Albumin (BSA) at 10 mg/ml was prepared in water. Two-fold serial dilutions of BSA solution was made and 0.1 ml each was incubated at room temperature with 0.9 ml of Biuret Reagent for 15 minutes. Absorbance at 550 nm was measured (Y-axis) and plotted against mg/ml protein (X-axis). A linear response of absorbance vs. protein concentration was observed.
The Biuret Protein Assay is a simple method for determining protein concentration. The amount of protein present in the sample is calculated based on the color change of the copper in the Biuret reagent in the presence of peptide bonds. The intensity of the color change is directly proportional to the number of peptide bonds present.
Product deScriPtioN code M262-500ML Size 500 ml See PAGe 197
Biuret Protein Assay Reagent
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PROTEOMICS 207
Protein Detection
Electrophoresis
Product Summary
NEXT GEL SpriNT NEXT GEL Fluorescent NEXT GEL Fluorescent Sprint NEXT GEL Turbo NEXT GEL 16 x16 cm gels Lp-NEXT GEL HTS-NEXT GEL Native Proteins Native NEXT GEL
Mini-gels
Acrylamide
In-gel Fluorescent Stain
Denatured Proteins
250 kDa 160 kDa 105 kDa 75 kDa 66.2 kDa 50 kDa 40.0 kDa 97.4 kDa
N XT G L
26.6 kDa 21.5/19.7 kDa
Agarose
A REVOLUTION IN RESOLUTION
Optimized for High Resolution SDS-Page Speed - Pour an Acrylamide Gel in One Minutes High Resolution - Resolve a 14.2 kDa Band from a 14.4 kDa Band on a Mini-gel Broad Range - Separate 3.5 kDa & 212 kDa proteins on same gel Convenience - No Stacking Gel required
35 kDa
30 kDa 25 kDa
For a complete listing and description of NEXT GEL products please refer to pages 153 - 158 of the Electrophoresis section.
*
15 kDa 14.4 kDa 10 kDa
SdS-PAGe with 10% NeXt GeL. Lane A and d: GE Healthcare Rainbow Molecular Weight Marker. Lane B: 50 g E. coli lysate. Lane c: 100 g E. coli lysate. Lane e: AMRESCO Mid/Low Range Protein Molecular Weight Marker.
Optimized for Running Large Format Gels (16 x 16 cm) in < 3 Hours Quality - Exceptional Resolution at < 1 kDa Convenient - No stacking gel required
Product deScriPtioN MoLecuLAr WeiGHt SePArAtioN rANGe 20-300 kDa code M323-100ML M323-500ML M313-100ML M313-500ML M310-100ML M310-500ML Size 100 ml 500 ml 100 ml 500 ml 100 ml 500 ml See PAGe 130
SdS-PAGe on turbo NeXt GeL. 16 x 16 cm x 1 mm 10% acrylamide gel run at 300 volts for 3 hours. Lane A: GE Healthcare Rainbow Molecular Weight Markers. Lane B: Total E. coli lysates (100 g). Lane c: AMRESCO Mid/Low Range Protein Molecular Weight Marker (Code: J450-200UL).
Turbo NEXT GEL, 7.5% Solution
Includes: NEXT GEL Running Buffer, 20X Each 30 ml will prepare a 16 cm x 16cm x 1.00 mm gel.
Turbo NEXT GEL, 10% Solution
10-200 kDa
130
Includes: NEXT GEL Running Buffer, 20X Each 30 ml will prepare a 16cm x 16cm x 1.00 mm gel.
Turbo NEXT GEL, 12.5% Solution
3.5-100 kDa
130
Includes: NEXT GEL Running Buffer, 20X Each 30 ml will prepare a 16 cm x 16 cm x 1.00 mm gel. A B c
*NEXT GEL products are patent pending
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Electrophoresis
Protein Detection
*
97.4 kDa 66.2 kDa
Optimized for Running Mini-gels in < 30 Minutes Fast Casting Time - Cast & Polymerize a 10 x 10 x 0.75 cm Mini-gel in < 15 Minutes Convenient - No Stacking Gel required Versatile - Compatible with Standard Staining Procedures & Downstream Applications
PRODUCT DESCRIPTION MoLecuLAr WeiGHt SePArAtioN rANGe 10-200 kDa code M312-100ML M312-500ML M311-100ML M311-500ML Size 100 ml 500 ml 100 ml 500 ml See PAGe 115
40.0 kDa 26.6 kDa 21.5/19.7 kDa 14.4 kDa Silver Stained SDS-PAGE on 10% Sprint Next Gel. Gel was run at 300 V for 30 min. Lane A: AMRESCO Mid/Low Range Protein MW Marker. Lane B: Total E. coli lysate, 50 g.
SPRINT NEXT GEL, 10% Solution
Includes: NEXT GEL Running Buffer, 20X Each 7.5 ml will prepare a 10 cm x 10 cm x 0.75 mm mini-gel.
SPRINT NEXT GEL, 12.5% Solution
3.5-100 kDa
115
Includes: NEXT GEL Running Buffer, 20X Each 7.5 ml will prepare a 10 cm x 10 cm x 0.75 mm mini-gel.
*
A
97.4 kDa 66.2 kDa
Optimized for Immediate Band Visualization Speed - Pour an Acrylamide Gel in One Minute Convenience - Fluorescent Stain incorporated into Gel Solution Enables Immediate Band Visualization Safe - No Need to Prepare or Dispose of Staining & Destaining Solutions
Product deScriPtioN MoLecuLAr WeiGHt SePArAtioN rANGe 10-200 kDa code M290-100ML-KIT M290-500ML-KIT M291-100ML-KIT M291-500ML-KIT Size 100 ml 500 ml 100 ml 500 ml See PAGe 54
40.0 kDa 26.6 kDa 19.7 kDa 14.4 kDa comparison of rapid fluorescent visualization with traditional coomassie staining. A 10% Fluorescent NEXT GEL was run using AMRESCOs Mid/Low Protein Marker (Code: J450-200UL) and NEXT GEL Running Buffer. The gel was run at 175 volts for 1 hour. The protein bands were first visualized by exposure to UV light for 3-5 minutes (A). The same gel (B) was subsequently stained with 0.1% Coomassie R-250 for 3 hours and destained according to standard methods. Fluorescent NEXT GEL offers comparable sensitivity to Coomassie R-250 (100 200 ng/ band) in a fraction of the time.
Fluorescent NEXT GEL, 10% Solution
Includes: NEXT GEL Running Buffer, 20X Each 10 ml will prepare a 10 cm x 10 cm x 0.75 mm mini-gel.
Fluorescent NEXT GEL, 12.5% Solution

*
3.5-100 kDa
54
Optimized for Fast Mini-gels & Immediate Band Visualization Speed - Cast & Run an SDS-PAGE Mini-gel in Under One Hour Convenience - Fluorescent Stain incorporated into Gel Solution Enables immediate Band Visualization Safe - No Need to Prepare or Dispose of Staining & Destaining Solutions
Product deScriPtioN MoLecuLAr WeiGHt SePArAtioN rANGe 10-200 kDa code M317-KIT-100ML M317-KIT-500ML M318-KIT-100ML M318-KIT-500ML Size 100 ml 500 ml 100 ml 500 ml See PAGe 54
Fluorescent SPRINT NEXT GEL, 10% Solution
Includes: NEXT GEL Running Buffer, 20X Each 10 ml will prepare a 10 cm x 10 cm x 0.75 mm mini-gel
Fluorescent SPRINT NEXT GEL, 12.5% Solution
3.5-100 kDa
54
rapid visualization of protein bands on 12.5% Fluorescent Sprint NeXt GeL. Gel was run at 300 V for 30 min., and bands visualized by exposure to UV light for 3 min. Lane A: AMRESCO Wide Range Protein MW Marker. Lane B: Total E. coli lysate. Lane c: Amresco Mid/Low Range Protein MW Marker.
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PROTEOMICS 209
Protein Detection
Electrophoresis
hts N
Optimized for High-Throughput Electrophoresis High-Throughput - Analyze up to 200 Protein Samples in Two Hours Non-Toxic & Biodegradable - Proprietary Agarose Blend Contains No Acrylamide
Product deScriPtioN Includes: HTS Agarose, 60g NEXT GEL Running Buffer, 20X, 2 Packs NEXT GEL Sample Buffer, 4X, 5 ml Contains sufficient reagents for 5 (25 cm x 25 cm) gels (smaller gels can be poured). code M281-KIT Size 1 Kit See PAGe 65, 199
XT G L
HTS NEXT GEL Electrophoresis Kit
MAiN PHoto: HTS NEXT GEL (4%)Stained with Coomassie R-250. iNSet PHoto: HTS NEXT GEL (4%)-Stained with Coomassie R-250. Protein Samples used: MW Standards (Wide) (Code: K494.
lP N
6400 kDa 3200 kDa 1600 kDa 800 kDa 400 kDa 200 kDa
Optimized for Large Protein SDS-PAGE Performance - Resolve 14,000 up to 6 Million Daltons on a Single Gel Versatile - Compatible with Downstream Applications including: Western Blotting, N-terminal Sequencing, Mass Spectometry & MALDI
Product deScriPtioN code M272-KIT Size 1 Kit See PAGe 73, 199
XT G L
Resolution of cross-linked myosin molecule on 1% Agarose HrP prepared with Fluorescent NeXt GeL Buffer. The gel was run at 100 volts for 1 hour. The protein bands were visualized by exposure to UV light for 3-5 minutes. The gel was then stained with 0.1% Coomassie R-250 for 3 hours and destained according to standard procedures (Right). The LP-NEXT GEL Fluorescent procedure offers enormous time savings with sensitivity comparable to Coomassie R-250.
LP-NEXT GEL Kit
Includes: Agarose HRP, 25 g NEXT GEL Running Buffer, 20X, 500 ml Fluorescent NEXT GEL Buffer, 20X, 125 ml NEXT GEL Sample Loading Buffer, 4X, 5 ml Sufficient materials to run 50 mini-gels.
natIve N
Optimized for Resolution of Both Basic & Acidic Native Proteins Easy-to-use - Non-denaturing Gel Electrophoresis Convenient - Utilizes Standard (Agarose) Electrophoresis Systems Versatile - Compatible with Downstream Applications including: Western Blotting, N-terminal Sequencing, Mass Spectometry & MALDI
A variety of proteins were separated using the Native NeXt GeL electrophoresis Kit. Lane 1: Trypsin Inhibitor. Lane 2: BSA. Lane 3: BSA. Lane 4: Blank. Lane 5: Glucose Oxidase. Lane 6: Glucose Oxidase. (Proteins stained with Coomassie Blue)
XT G L
Product deScriPtioN
code M271-KIT
Size 1 Kit
See PAGe 85, 200
Native NEXT GEL Electrophoresis Kit
Includes: Agarose HRP, 25g NEXT GEL Running Buffer, 20X, 500 ml NEXT GEL Sample Buffer, 4X, 5 ml Contains sufficient reagents to perform 30 50 mini gels.
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Protein Transfer
Blotting
Protein Detection
The definition of the word detection is most closely linked to the term discovery and discovery is the cornerstone to the life sciences. Although broadly speaking, detection can be viewed as the discovery of anything, in the molecular biology sense, detection largely focuses on the identification of the major biomolecules, DNA, RNA and proteins. Over the years, detection of DNA, RNA and proteins has evolved into not only answering the question of whether a biomolecule is present but specifically, which molecule is present. Commonly referred to as blotting or hybridization, the technique was originally developed in 1975 by British molecular biologist E. M. Southern. The original technique was optimized for the identification of DNA fragments, and appropriately named the Southern Blot. In years to follow, the technique was modified for the detection of RNA, known as the Northern Blot and for protein, known as the Western Blot. With the increasing advances in automation married with the information and software technologies, the birth and growth of microarray technologies has been possible. Based on the traditional blotting methodologies, the microarray and protein array technologies continue to be developed and refined for use as not only research and investigational tools but tools for the fast and accurate diagnosis and predisposition of disease. The fundamental hybridization or blotting technique can be summarized as the very specific interaction of a known fragment of DNA, RNA or antibody with an unknown sample, thereby revealing its identity. Typically the procedures begin with the electrophoretic separation of the molecules, followed by the transfer of these molecules to an immobilized membrane. This membrane is then probed with the known DNA, RNA or antibody, which will bind to the membrane only if the specific target is present on the blot. This presence is revealed through a number of visible modes; including substrate colorimetric reactions, fluorescent molecules, radioactive detection and chemiluminescent substrates.
totalblot+ blottIng membranes

High Binding Capacity Nylon Membranes are Mechanically Strong & Resistant to Tearing or Cracking PVDF Membranes are Well Suited for Western Protein Transfer
Product deScriPtioN MeMBrANe Size 15 x 15 cm 10 x 10 cm 30 cm x 3 m (1 Roll) 15 x 15 cm 10 x 10 cm 30 cm x 3 m (1 Roll) code E576-5X15CMSQ E576-10X10CMSQ E576-1ROLL E578-5X15CMSQ E578-10X10CMSQ E578-1ROLL Size 5 10 1 Roll 5 10 1 Roll See PAGe 124
Related Products
Albumin Blocking Buffers Wash Buffers Horse radish Peroxidase Alkaline Phosphatase Western Blotting Kits
TotalBLOT+ Nylon Membranes
TotalBLOT+ PVDF Membranes
124
transFer buFFers
Product deScriPtioN deScriPtioN Buffer used for the transfer of protein from SDS-PAGE gels to membranes for protein sequencing or Western Blotting Buffer specifically formulated for the transfer of proteins from NEXT GELs to membranes for protein sequencing or Western Blotting coNceNtrAtioN code 10X K872-500ML Size 500 ml See PAGe 34
CAPS Transfer Buffer, 10X
NEXT GEL Transfer Buffer
10X
M279-500ML
500 ml
200
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PROTEOMICS 211
Blotting
Blocking
Technical Tip
Blocking is usually performed using a protein like milk or serum in order to block nonspecific sites on the membrane. Blocking should improve the sensitivity of the assay by reducing the background without interfering with the epitope for antibody binding. Recommendations - PBS interferes with alkaline phosphatase. Therefore, avoid using PBS for blocking when using alkaline phosphatase. - Do not use milk to block membranes when using an avidin-biotin system. Milk contains biotin.
albumIn, bovIne serum (bsa)

Extensive Line of BSA for a Wide Variety of Applications
Product NAMe deScriPtioN Nuclease and protease-free powder, suitable for use in most molecular biology applications. A heat shock isolation product provided as a fine, white powder in a salt-free form (pH 5.2) for low salt applications. This salt-free preparation is the purest material best suited for the most sensitive biochemical and diagnostic assays. It is DNase and RNase-free, virtually free of globulins and other interfering activities, making it an excellent stabilizer for molecular biology enzymes. Contains 0.1% sodium azide as preservative and strictly tested for RNase, DNase and Protease making it suitable for sensitive applications. Concentrated 20% BSA solution (Albumin, Bovine, Fraction V Cold Alcohol Isolation) in 0.85% sodium chloride and contains 0.1% sodium azide (as a preservative for stability). Concentrated 30% BSA solution (Albumin, Bovine, Fraction V Cold Alcohol Isolation) in 0.85% sodium chloride and contains 0.1% sodium azide (as a preservative for stability). code 0332-25G 0332-100G 0175-25G 0175-100G Size 25 g 100 g 25 g 100 g See PAGe 20
Albumin, Bovine Serum, Fraction V, Heat Shock Isolation Albumin, Bovine Serum, Fraction V, Salt-free Form
20
Albumin, Bovine Serum, Fraction V, Cold Alcohol Isolation
0903-5G 0903-10G
5g 10 g
20
Albumin, Bovine Serum, 20 mg/ml Solution
J642-1ML J642-5ML
1 ml 5 ml
20
Albumin, Bovine Serum, 20 % Solution
K720-50ML K720-500ML
50 ml 500 ml
21
Albumin, Bovine Serum, 30 % Solution
K719-50ML K719-500ML
50 ml 500 ml
21
WeSterN BLottiNG ProtocoL

1. Run gel 2. Trasfer protein to membrane 3. Block non-specific sites on the membrane 4. Wash membrane
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Blocking
Blotting
blockIng buFFers
High Quality Liquid & Powdered Blocking Buffers Optimize Assay Performance & Improve Sensitivity Ready-To-use Pre-measured Pouches Provide Maximum Convenience
BIO-BLOCK is a blocking buffer optimized for blocking non-specific binding sites on positively charged nylon or PVDF membranes in nucleic acid or protein blotting applications. The resulting lower background increases the signal-to-noise ratio to improve sensitivity and specificity of your blots. BIOBLOCK is available in either 1X PBS or 1X TBS to ensure compatibility with most enzyme systems. Each pack of AMRESCOs Powdered Blocking Buffers will make one liter of 1X solution. These reagents, including TBS with Non-Fat Powdered Milk, TBS with BSA, PBS with Non-Fat Powdered Milk, PBS with BSA and TBS with Tween 20, are designed for use in Western blotting.
Product deScriPtioN code E671-1L E667-1L M232-39.8G-5PK M233-19.8G-5PK M245-10.4G-5PK M230-42G-5PK M231-22G-5PK M235-12.5G-5PK M247-6PK Size 1L 1L 5 pack 5 pack 5 pack 5 pack 5 pack 5 pack 6 pack See PAGe 28 28 200 200 200 202 202 202 29
BIO-BLOCK in 1X PBS BIO-BLOCK in 1X TBS PBS with Non-Fat Powdered Milk PBS with BSA PBS with Tween 20 TBS with Non-Fat Powdered Milk TBS with BSA TBS with Tween 20 Blocking Agent Sampler Pack
Includes 1 of each TBS & PBS pack listed above Excludes Bio-Block & Fish Gelatin Products
Fish gelatin blocking of Western blots - courtesy of dr. eric carlson, case Western reserve university, cleveland, ohio. Whole corneal extracts were immunoprecipitated and resolved by SDS-PAGE on a 10% Fluorescent SPRINT NEXT GEL. Bands were transferred to membrane and blocked with a 5% Fish Gelatin Blocking Buffer in phosphate-buffered saline and Tween 20 (PBST). Primary and secondary antibody incubations were perfomed in PBST prior to development with ECL. The blot was slightly overexposed to show the extremely low background in the control samples (Lanes 1-4) and the experimental samples (Lanes 5-8).
Fish Gelatin Blocking Buffer, 10% Solution
M319-100ML M319-500ML
100 ml 500 ml
53
5. Incubate membrane with primary & secondary antibodies
6. Add the detection reagents & visualize bands
Wash
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PROTEOMICS 213
Blotting
Wash Buffers & Enzymes
Wash buFFers & detergents

Prevent High Background by Effectively Removing Unbound Reagents Convenient Concentrated PBS & TBS Ideal for Blotting & ELISA Applications
Following each step in a Western blotting procedure, a wash step is required in order to remove unbound reagents. PBS (Phosphate Buffered Saline) and TBS (Tris Buffered Saline) with a detergent such as Tween 20 are commonly used wash buffers in immunoassay procedures. Detergents are added to blocking and wash buffers in order to reduce non-specific binding thereby improving the sensitivity of the assay. AMRESCO offers a comprehensive line of buffers and detergents ideal for use in Western blotting, ELISA, etc
Product deScriPtioN code K875-500ML K873-500ML 0780-10L E404-100TABS E404-200TABS E703-500ML E703-1L E715-500ML E715-1L 0788-2PK J640-4L K859-100TABS K859-200TABS M228-10ML-5PK Size 500 ml 500 ml 10 L* 100 Tablets** 200 Tablets 500 ml 1L 500 ml 1L 2 x 1 of 20X 4L 100 Tablets 200 Tablets 5 pack See PAGe 29 29 96 96 96 97 120 120 120 130
Peroxide-free detergents
Blot Wash Buffer, 20X PBS Tween 20 Blot Wash Buffer, 20X TBS Tween 20 PBS Powder PBS Tablets PBS, 20X Liquid Concentrate
PBS tablets
PBS, with Tween, pH 7.5 TBS Buffer, 20X Ready-Pack TBS, 20X Liquid Concentrate TBS Buffer Tablets Tween 20, 10% Solution, Peroxide-free 10 ml ampoules
*There is sufficient powder to prepare 10L of a 1X solution by using 9.88 g/L **Each tablet prepares 100 ml of 1X PBS when diluted in water
enzymes
The common use of ELISA, immunoblotting and immunochemical techniques requires the use of high quality enzymes and substrates for the detection of biomolecules. Alkaline phosphatase and horseradish peroxidase are the most commonly employed enzymes for these highly sensitive detection methods. AMRESCO supplies both enzymes and a line of specially prepared colorimetric substrates for each enzyme in powder, tablet and ready-to-use liquid stable forms.
Product NAMe deScriPtioN An enzyme commonly used for the removal of terminal phosphates from the 5 ends of DNA or RNA strands to prevent re-annealing of cohesive ends and facilitate future radiolabeling of the strands. Is most often conjugated to a secondary antibody or cellular component (avidin or biotin). HRP catalyzes the decomposition of hydrogen peroxide in solution causing the oxidation of a number of substrates. Used extensively as a label for immunohistological assays and ELISA. Its exceptional stability in powdered and solution form enhances its desirability as an enzyme conjugate. SPeciFic ActiVitY 3000 U/mg protein code E552-1000UNITS E552-5000UNITS Size 1000 Units 5000 Units See PAGe 21
Alkaline Phosphatase (Alk Phos)
Peroxidase, Horseradish Conjugation Grade (HRP)
250 Purpurogallin Units/mg
0343-10000U 0343-25000U
10,000 Units 25,000 Units
93
Peroxidase, Horseradish Reagent Grade (HRP) b-Galactosidase
100 Purpurogallin Units/mg
0417-25000U 0417-100000U 0641-1000U 0641-5000U
25,000 Units 100,000 Units 1000 Units 5000 Units
93
An enzyme responsible for the degradation of galactosyl b-linkages in glycoproteins, glycolipids and polysaccharides. Can be conjugated to a variety of probes for the chromogenic detection of target sequences using X-Gal.
600 U/mg protein
56
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Labeling Reagents
Blotting
ProteIn & antIbody labelIng reagents

Optimize the Sensitivity of Your Assay Using AMRESCO Labeling Reagents
Product deScriPtioN APPLicAtioN A 66 kDa glycoprotein containing four identical subunits with high binding affinity for biotin. Stable over wide pH and temperature ranges. Used in detection and protein purification methods because it can bind one molecule of biotin with each subunit. The strong avidinbiotin interaction is often utilized in affinity chromatography, ELISA, immunohistochemistry and Western Blotting. A small biomolecule, 244 Daltons, that has high binding affinity to avidin and streptavidin often used to label antibodies and enzymes. Several molecules of biotin can react with a single protein molecule to increase the sensitivity at the site of detection in ELISA, immunohistochemistry and Western Blotting. Features an excitation wavelength of 494 nm and an emission wavelength of 520 nm. Used widely in immunohistochemistry, flow cytometry, FACS analysis and molecular structure and functional studies. Features an excitation wavelength of 544 nm and an emission wavelength of 576 nm. Used widely in immunohistochemistry, flow cytometry, FACS analysis and molecular structure and functional studies. A 60 kDa tetrameric protein that exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit, streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting. code 0866-5MG Size 5 mg See PAGe 26
Avidin, Chicken Egg White
D-Biotin
0340-1G 0340-5G
1g 5g
28
Fluorescein Isothiocyanate
0633-500MG
500 mg
53
Rhodamine B Isothiocyanate
0731-100MG 0731-250MG
100 mg 250 mg
105
Streptavidin (Streptomyces avidinii)
E497-1MG E497-5MG
1 mg 5 mg
116
www.amresco-inc.com
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PROTEOMICS 215
Blotting
Chromogenic Kits
Western max chromogenIc kIts

Complete Anti-Mouse & Anti-Rabbit Detection Kits for Immunoblotting Rapid & Sensitive Detection Consistent & Reproducible Results
Product deScriPtioN Includes: AP Conjugated Anti-Mouse Antibody BCIP/NBT Substrate Tablets IgG positive control Dilution Buffer Powder Tween 20 BSA, Fraction V Western MAX Guide with Protocol Contains sufficient reagents for forty 10 cm x 10 cm immunoblots. Includes: AP Conjugated Anti-Rabbit Antibody BCIP/NBT Substrate Tablets IgG positive control Dilution Buffer Powder Tween 20 BSA, Fraction V Western MAX Guide with Protocol Contains sufficient reagents for forty 10 cm x 10 cm immunoblots. Includes: HRP Conjugated Anti-Mouse Antibody DAB Substrate Tablets IgG positive control Dilution Buffer Powder Tween 20 BSA, Fraction V Hydrogen Peroxide Western MAX Guide with Protocol Contains sufficient reagents for forty 10 cm x 10 cm immunoblots. Includes: HRP Conjugated Anti-Rabbit Antibody DAB Substrate Tablets IgG positive control Dilution Buffer Powder Tween 20 BSA, Fraction V Hydrogen Peroxide Western MAX Guide with Protocol Contains sufficient reagents for forty 10 cm x 10 cm immunoblots. code K203-1KIT Size 1 Kit See PAGe 134
Western MAX AP Kit, Anti-Mouse
Western MAX AP Kit, Anti-Rabbit
K204-1KIT
1 Kit
134
Western MAX HRP Kit, Anti-Mouse
K205-1KIT
1 Kit
134
Related Products
Acrylamide Ammonium Persulfate teMed Protein MW Markers ziP reversible Protein Stain
Western MAX HRP Kit, Anti-Rabbit
K206-1KIT
1 Kit
134
216 PROTEOMICS
1-800-829-2805
www.amresco-inc.com
Chemiluminescent Kits
Blotting
Western max chemIlumInescent kIts

Rapid Chemiluminescent Western Blotting Kits Offer Superior Sensitivity These Kits Minimize Species Cross Reactivity while Offering High Resolution & Low Background Flexible Configurations Enable Detection of Mouse or Rabbit Antibodies Using Either Alkaline Phosphatase or Horseradish Peroxidase Can be Used with PVDF or Nitrocellulose Membranes
AMRESCO now offers chemiluminescent Western blotting kits that offer rapid and sensitive detection of either rabbit or mouse antibodies complexed with antigen immobilized on PVDF or nitrocellulose membranes. The highly purified secondary antibody conjugates and matching reagents provide superior sensitivity, minimize species cross reactivity and reduce lot-to-lot variation. All solutions required for blocking and washing the membrane and for diluting antibodies are provided. The chemiluminescent substrate is provided as a stable two component solution which emits light when combined with a Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP) labeled antibody. Results are recorded on X-ray film.
Product deScriPtioN Includes: AP Conjugated Anti-Mouse Antibody VisiGlo AP Chemiluminescent Substrate IgG positive control Dilution Buffer Powder Tween 20 BSA, Fraction V Western MAX Guide with Protocol Contains sufficient reagents for forty 10 cm x 10 cm immunoblots. Includes: AP Conjugated Anti-Rabbit Antibody VisiGlo AP Chemiluminescent Substrate IgG positive control Dilution Buffer Powder Tween 20 BSA, Fraction V Western MAX Guide with Protocol Contains sufficient reagents for forty 10 cm x 10 cm immunoblots. Includes: HRP Conjugated Anti-Mouse Antibody VisiGlo HRP Chemiluminescent Substrate IgG positive control Dilution Buffer Powder Tween 20 BSA, Fraction V Western MAX Guide with Protocol Contains sufficient reagents for forty 10 cm x 10 cm immunoblots. Includes: HRP Conjugated Anti-Rabbit Antibody VisiGlo HRP Chemiluminescent Substrate IgG positive control Dilution Buffer Powder Tween 20 BSA, Fraction V Western MAX Guide with Protocol Contains sufficient reagents for forty 10 cm x 10 cm immunoblots. code N221-KIT Size 1 Kit See PAGe 134
Bovine serum albumin was run on an electrophoresis gel and transferred to a nitrocellulose membrane following standard Western blotting procedures. The blot was developed using AMRESCOs Western MAX HRP Chemiluminescent Kit, Anti-Mouse. The chemiluminscent bands corresponding to bovine serum albumin were detected following a ten second exposure to X-ray film.
Western MAX Chemiluminescent AP Kit, Anti-Mouse
Technical Tip
N220-KIT 1 Kit 134
Western MAX Chemiluminescent AP Kit, Anti-Rabbit
Weak or low signal Western blots can be caused by proteins that did not transfer properly to the membrane. Precautions: - Use ProAct Membrane Stain (Code: M282-1L) to check that the proteins transferred to the membrane. - After transfer is complete, stain the gel with a total protein stain (Blue BANDit Protein Stain, Code: K2171L) to determine transfer efficiency.
Western MAX Chemiluminescent HRP Kit, Anti-Mouse
N222-1KIT
1 Kit
134
Western MAX Chemiluminescent HRP Kit, Anti-Rabbit
N223-1KIT
1 Kit
134
www.amresco-inc.com
1-800-829-2805
PROTEOMICS 217
Blotting
Chemiluminescent Substrates
vIsIglo & vIsIglo Plus chemIlumInescent substrates

Extremely Sensitive Substrates - Save precious target proteins & antibodies Low Background without Compromising Signal Intensity
VisiGlo PLuS AP Substrate
VisiGlo Substrates for HRP Offer a Dynamic Range that is Linear for a Longer Period of Time than Other Chemiluminescent Substrates VisiGlo Substrates for AP Feature Long Glow Times of up to 5 Days - Allows for repeated exposure or luminosity reading
visiglo comParIson chart VisiGlo HrP Activation Enzyme Detection Limit Working Solution Emission Duration Stability Recommended Membranes Detection Methods Horseradish Peroxidase (HRP) Picogram Mix 2 components 1-2 hours > 1 year at 4 C Nitrocellulose and PVDF Film VisiGlo PLuS HrP Horseradish Peroxidase (HRP) Femtogram Mix 2 components 4-8 hours > 1 year at 4 C Nitrocellulose and PVDF Film/ Chemiluminescent Imager VisiGlo AP Alkaline Phosphatase (AP) Picogram Ready-to-use > 5 days 2 years at 4 C Nitrocellulose and PVDF Film/ Chemiluminescent Imager VisiGlo PLuS AP Alkaline Phosphatase (AP) Femtogram Ready-to-use > 5 days 2 years at 4 C Nitrocellulose and PVDF Film/ Chemiluminescent Imager
VisiGlo AP Substrate
cdP-Star chemiluminescent Substrate

A comparison of low-end sensitivity using VisiGlo PLUS AP Substrate, VisiGlo AP Substrate and CDP-Star Chemiluminescent Substrate on PVDF membranes. Five-fold serial dilutions of Mouse IgG (2 ng - 3.2 pg) were separated by SDS-PAGE and transferred to the membranes. Protein was detected using a 1:1,000 dilution of biotin-labeled goat antimouse IgG, a 1:10,000 dilution of AP-labeled streptavidin and each respective substrate. CDP-Star was mixed with NitroBlock-II Luminescence Enhancer before applying it to the nitrocellulose blot. Film was exposed for 10 minutes.
vIsIglo aP & vIsIglo Plus aP chemIlumInescent substrates

VisiGlo AP and VisiGlo PLUS AP substrate are the chemiluminescent substrates of choice for easy, reliable detection of alkaline-phosphatase (AP) in Western blotting or ELISA. VisiGlo AP and VisiGlo PLUS AP substrates are ready-to-use, one component systems requiring no special blocking solutions. These substrates are stable for two years at 4C eliminating concerns about rapidly degrading substrates.
Product deScriPtioN code N216-100ML N217-100ML Size 100 ml 100 ml See PAGe 133 133
VisiGlo AP Chemiluminescent Substrate

Sufficient material for ~ 2000 cm2 of membrane.
VisiGlo PLUS AP Chemiluminescent Substrate

Sufficient material for ~ 2000 cm2 of membrane.
218 PROTEOMICS
1-800-829-2805
www.amresco-inc.com
Chemiluminescent Substrates
Blotting
vIsIglo hrP & vIsIglo Plus hrP chemIlumInescent substrates

VisiGlo HRP and VisiGlo PLUS HRP are luminol-based chemiluminescent substrates designed for the rapid and sensitive detection of peroxidase labeled-conjugates. They offer increased sensitivity compared to chromogenic substrates in both blotting and ELISA applications. VisiGlo HRP and VisiGlo PLUS HRP can be detected using X-ray film and chemiluminescent imagers in blotting applications (see Comparison Chart below) or using a luminometer for ELISA. VisiGlo PLUS HRP is ideal for those situations where the protein of interest is expressed in low abundance. Strong signal is achieved with the use of reduced amounts of precious target and antibodies.
Densitometry for VisiGlo vs. ECL

VisiGlo ECL
Comparison of VisiGlo Plus and ECL chemiluminescent substrate sensitivity. Serial dilutions of Mouse IgG (1 ng 31 pg) were separated by SDS-PAGE and transferred to PVDF membranes. Protein detection was performed using HRP-labeled anti-mouse antibody and each respective substrate. Film was exposed for 10 minutes and analyzed for sensitivity and signal to noise ratios. A) VisiGLO PLUS HRP, B) ECL Plus, C) ECL Advance. Sensitivity of VisiGlo HrP. Serial dilutions of mouse (6.1-0.024 pg) IgG were run on SDS-PAGE and transferred to membrane. Blots were developed with VisiGlo HRP and exposed to X-ray film for 10 minutes.
Densitometry of VisiGlo Plus HRP vs. Competitor Substrates

90000 80000 VisiGlo PLUS HRP West Femto West Dura ECL Adv ECL Plus
Relative Image Density
70000 60000 50000 40000 30000 20000 1 0000
Product deScriPtioN
code N218-KIT N218-S-KIT N218-SAMPLE-KIT N219-KIT
Size 1 Kit
See PAGe 133
VisiGlo HRP Chemiluminescent Substrate Kit
VisiGlo PLUS HRP Chemiluminescent Substrate Kit

Sufficient material for ~ 2400 cm of membrane.
2
1 Kit
133
0 3.906 7.813 15.63 31.25 62.5 125
Concentration (pg)
www.amresco-inc.com
1-800-829-2805
PROTEOMICS 219
Blotting
Stripping Buffers
gentle revIeW buFFer

I tried Gentle [ReView] for 30 mins at 37 C with shaking, and I got 100% removal of both primary and secondary [antibodies]. ... I would love to use this for my regular projects.
Odor-free, Room-temperature Procedure - No hood needed! Convenient Ready-To-use Formulation Minimize Loss of Valuable Antigen
Samarjit Das, Ph.D. Department of Cardiac Pathology Johns Hopkins University School of Medicine, Baltimore, MD
Differential expression pattern of IP3 Receptor Type 1 and Type 3 in Dexamethasone (Dex) treated WEHI 7.2 mouse T cell lymphoma line. Courtesy of Dr. Karen McColl, Case Western Reserve University, Cleveland, Ohio. A single Western blot was used to analyse the expression of IP3 Receptor Type 1 and Type 3 during Dex treatment.. The blot was probed with antibodies to IP3 Recepetor Type 1 and then stripped for 45 minutes with Gentle Review Stripping Buffer prior to reprobing with antibodies to IP3 Receptor Type 3. The different expression pattern obtained with each antibody demonstrates the effectiveness of the stripping and reprobing procedure. type 1: expression increases with Dex treatment.. type 3: expression remains constant with Dex treatment. Actin: Sample loading control.
Product deScriPtioN
code N552-1L
Size 1 liter
See PAGe 58
Gentle ReView Buffer
Related Products
Membranes tris t PBS tween t VisiGlo chemiluminescent Substrates
revIeW buFFer solutIon

Save Money & Time with Multiple Rounds of Stripping & Reprobing of a Single Blot Confirm Images Derived from the Same or Different Antibodies in the Same Blot
Product deScriPtioN code N166-1L Size 1 liter See PAGe 105
ReView Buffer Solution
v vantage revIeW strIPPIng buFFer

Remove High-affinity Antibodies with Minimal Loss of Bound Antigen Conserve Scarce Antigen & Valuable Antibodies Supplied as a Ready-to-use Solution
Detection of IP3 Type 1 and Type 3 Receptors on a Single Western Blot

16 hr 24 hr 24 hr 32 hr 32 hr 40 hr 48 hr 48 hr 72 hr 0 hr 0 hr
Type 1 Type 3 Actin Bcl-2
24 hr Veh 24 hr Veh
Wehi 7.2
24 hr 24 hr
0 hr
differential expression of iP3 type 1 and type 3 receptors in 1 M dexamethasone treated WeHi 7.2 mouse t cell lymphoma line. Courtesy of Dr. Karen McColl, Case Western Reserve University, Cleveland, Ohio. A single Western blot was probed with antibodies to the IP3 receptor Type I and then stripped for 65 minutes with Vantage ReView buffer. After removal of primary and secondary antibodies, the blot was reprobed with IP3 receptor Type 3 antibody. Successful removal of Type 1 antibody is demonstrated by the differential expression of Type 1 and Type 3 antibodies on the same blot.
4 hr 4 hr
8 hr 8 hr
Product deScriPtioN
code
Size 500 ml
See PAGe 133
Vantage ReView Stripping Buffer
N592-500ML
220 PROTEOMICS
1-800-829-2805
www.amresco-inc.com

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proteomics

$39.50 $131.30 $207.20

$42.10 $72.00 $115.60

BES FREE ACID

$28.20 $52.10 $103.20

BIURET PROTEIN ASSAY REAGENT

$45.40 $138.40 $268.70

BLUE BANDIT PROTEIN STAIN

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BRADFORD METHOD PROTEIN ASSAY KIT

COOMASSIE BRILLIANT BLUE R-250

$22.80 $43.50 $66.70

DANSYL CHLORIDE BRIj 35, 10% SOLUTION

Proteomics, 205, Buffers & Detergents, 230

DETERGENT SAMPLER, PEROXIDE FREE

Proteomics, 205 , Buffers & Detergents, 230

BROMOPHENOL BLUE SODIUM SALT

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EDTA DISODIUM SALT DIHYDRATE

$49.30 $75.30 $165.50

$43.50 $160.60 $268.30

FORMALDEHYDE, 37% SOLUTION

GLUTARALDEHYDE, 50% SOLUTION

COOMASSIE BRILLIANT BLUE G-250

$22.20 $34.30 $61.30

GLYCEROL, 20% STERILE SOLUTION

IMIDAZOLE CHROMATOGRAPHY BUFFER, 5X

GLYCINE CHROMATOGRAPHY BUFFER, 5X

IODOACETIC ACID SODIUM SALT

100 g 250 g 500 g

$35.10 $62.50 $107.80

GUANIDINE HYDROCHLORIDE, 8M SOLUTION

LP-NEXT GEL ELECTROPHORESIS KIT

HTS NEXT GEL KIT

IEF ANODE BUFFER, 20X

IEF CATHODE BUFFER, 20X

MOPS FREE ACID

MOPS SODIUM SALT

$32.30 $105.00 $125.00

NATIVE NEXT GEL ELECTROPHORESIS KIT

NON-FAT POWDERED MILK

NONIDET P-40 SUBSTITUTE

NEXT GEL, 5% SOLUTION

$14.10 $21.50 $68.80

Proteomics, 205, Buffers & Detergents, 89

Electrophoresis, 153, Proteomics, 208

NEXT GEL, 7.5% SOLUTION

$25.50 $40.30 $159.00

Electrophoresis, 153, Proteomics, 208

NEXT GEL, 10% SOLUTION

PBS WITH BSA

Electrophoresis, 153, Proteomics, 208

NEXT GEL, 12.5% SOLUTION

PBS WITH NON-FAT POWDERED MILK

Electrophoresis, 153, Proteomics, 208

NEXT GEL, 15% SOLUTION

PBS WITH TWEEN 20

Electrophoresis, 153, Proteomics, 208

NEXT GEL SAMPLE LOADING BUFFER, 4X

NEXT GEL TRANSFER BUFFER

PROTEASE INHIBITOR COCKTAIL, PLANT

PROTEIN GEL LOADING BUFFER, 2X

PROTEIN STAIN, IN GEL

PHOSPHATE CHROMATOGRAPHY BUFFER, 5X