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EX-CELL™TM302
EX-CELL 302
CHO-K1 Multiple Passage
CHO-K1 Multiple Passage
6e+6
6e+6 1.0
1.0
5e+6
5e+6
Culture Density (cell/mL)
0.8
0.8
4e+6
4e+6
Viability (%)
0.6
0.6
3e+6
3e+6
Viability (%
0.2
0.2
1e+6
1e+6
00 0.0
0.0
00 5
5 10
10 15
15 20
20 25
25 30
30 35
35 40
40 45
45
Culture Days Culture Growth
Culture Days Culture Growth
Maximum Culture Density
Maximum Culture Density
Culture Viablility
Culture Visibility
Conclusions
Data presented here demonstrate extended cellular growth
in EX-CELLTM 302 medium for eight (8) days in spinner batch
cultures. Cultures of CHO-K1 cells achieved an average
maximum cell density of approximately 3.5 x 106 cells/mL
with exponential doubling times in the range of 20 hours
while maintaining viabilities greater than 70%.
EX-CELLTM 302 contains low levels (< 100 µg/L total) of
recombinant proteins. CHO-K1 cells, cryopreserved and
adapted to serum-free growth in EX-CELL TM 301, were
transferred directly into EX-CELLTM 302 without an adaptation
period. SAFC Biosciences’ regulatory friendly EX-CELLTM 302
is suitable for the production of human therapeutic products
since it does not contain animal-derived proteins or other
undesirable components.
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