MICROSCOPE COMPONENTS GUIDE

Choosing The Ideal UIS2 Optics Components For Your Equipment

2008-10

The wide range of Olympus components introduced here allows users in such diverse fields as research, inspection and production to take advantage of the quality, flexibility and outstanding optical performance of the UIS2 Optical System. That's why installing Olympus microscope components is, quite simply, the right choice for your equipment.

CONTENTS

WELCOME TO UIS2/UIS OPTICS ---------------------------------------------- 3 4

EYEPIECES/FILAR MICROMETER EYEPIECE --------------------------------- 33 Widefield eyepieces WHN10x, WHN10x-H, CROSSWHN10x, WH15x --------------------- 33 SWH10x-H, MICROSWH10x, CROSSSWH10x ------------------------------ 33 U-OSM --------------------------------------- 33

SYSTEM DIAGRAM --------------------------------------------------------------- 5 6

Super widefield eyepieces Filar micrometer eyepiece

UIS2/UIS OBJECTIVE LENSES ----------------------------------------------- 7 18

NEW

NEW

M Plan Apochromat MPLAPON series -------------------------------8 M Plan SemiApochromat MPLFLN series -------------------------------- 9 Long WD M Plan SemiApochromat LMPLFLN series ---------------------------- 10 M Plan Achromat MPLN series --------------------------------- 11 LCD Long WD M Plan SemiApochromat LCPLFLN-LCD series -------------------------12 Super Long WD M Plan Achromat SLMPLN series -------------------------------13 IR Long WD M Plan Achromat LMPlan-IR series -----------------------------14 IR M Plan Achromat MPlan-IR -------------------------------------14 M Plan SemiApochromat BD MPLFLN-BD series ---------------------------15 M Plan SemiApochromat BDP MPLFLN-BDP series --------------------------16 Long WD M Plan SemiApochromat BD LMPLFLN-BD series --------------------------17 M Plan Achromat BD MPLN-BD series ------------------------------18

REVOLVING NOSEPIECES ---------------------------------------------------- 34 35 Quintuple revolving nosepiece U-5RE-2-------------------------------------- 34 Sextuple revolving nosepiece with slider slot for DIC U-D6RE--------------------------------------- 34 Sextuple revolving nosepiece with slider slot for DIC with ESD treatment U-D6RE-ESD --------------------------------- 34 Septuple revolving nosepiece with slider slot for DIC U-D7RE -------------------------------------- 34 Centerable quadruple revolving nosepiece with slider slot for DIC U-P4RE -------------------------------------- 34 Centerable sextuple revolving nosepiece with slider slot for DIC U-P6RE -------------------------------------- 34 Quintuple revolving nosepiece for BF/DF U-5BDRE ------------------------------------ 35 Quintuple revolving nosepiece for BF/DF with slider slot for DIC U-D5BDRE ----------------------------------- 35 Sextuple revolving nosepiece for BF/DF with slider slot for DIC/ U-D6BDRE ----------------------------------- 35 Centerable quintuple revolving nosepiece U-P5BDRE ----------------------------------- 35 Adapter to mount BF objectives BD-M-AD ------------------------------------ 35

MICROSCOPE SYSTEM BXFM ---------------------------------------------- 19 22 BXFM frame BXFM BXFM-S Universal stand type 2 Compact stand Large stand BXFM-F -------------------------------------- 19 BXFM-F+BXFM-ILH+BXFM-ILHSPU---------- 20 BXFM-F+BXFM-ILHS ------------------------ 21 SZ2-STU2 ----------------------------------- 22 U-ST ----------------------------------------- 22 SZ-STL -------------------------------------- 22

ILLUMINATION UNITS --------------------------------------------------------- 23 25 Reflected light illuminator for BF/DF BX-RLA2 ------------------------------------- 23 Universal reflected light illuminator BX-URA2 ------------------------------------ 23 Reflected light illuminators for BF BX-KMA/BX-KMA-ESD ----------------------- 24 Reflected light illuminator for BF U-KMAS ------------------------------------- 25

VIDEO CAMERA ADAPTERS ------------------------------------------------- 36 37 C-mount video camera ports Video camera mount adapters Video camera port U-TV0.25xC, U-TV0.35xC-2, U-TV0.5xC-3, U-TV0.63xC ------------------- 36 U-CMAD3, U-BMAD, U-SMAD, U-TMAD, U-FMT ----------------------------- 37 U-TV1x-2 ------------------------------------ 37

LAMP HOUSING & ACCESSORIES ----------------------------------------- 26 28 75W xenon apo lamp housing U-LH75XEAPO ------------------------------- 26 100W mercury apo lamp housing U-LH100HGAPO ----------------------------- 26 100W mercury lamp housing U-LH100HG ---------------------------------- 26 100W halogen lamp housings U-LH100-3/U-LH100IR/U-LH100L-3 -------- 26 External power supply TH4-100/200 -------------------------------- 27 Hand switch TH4-HS -------------------------------------- 27 Extension cord U-RMT --------------------------------------- 27 DF converter for BX-URA2 U-RCV --------------------------------------- 27 Fiber adapter for reflected light observation U-LGAD -------------------------------------- 27 Transmitted light guide adapter SZX-TLGAD ---------------------------------- 27 Light source LG-PS2 -------------------------------------- 28 Light guide LG-SF ---------------------------------------- 28 Double lamp house adapter U-DULHA ------------------------------------ 28

MOTORIZED UNITS------------------------------------------------------------- 38 41 Motorized BF/DF reflected light illuminator+motorized Nomarski DIC sextuple revolving nosepiece+100W halogen lamp housing BX-RLAA+U-D6REMC+U-LH100-3 ---------- 38 Motorized universal reflected light illuminator BX-RFAA ------------------------------------- 38 Motorized sextuple BD revolving nosepiece with slider slot for DIC U-D6BDREMC ------------------------------- 39 Motorized centerable quintuple revolving nosepiece with slider slot for DIC U-P5BDREMC -------------------------------- 39 Motorized quintuple BD revolving nosepiece with slider slot for DIC U-D5BDREMC ------------------------------- 39 Motorized sextuple revolving nosepiece with slider slot for DIC U-D6REMC ---------------------------------- 39 Motorized centerable quintuple revolving nosepiece with slider slot for DIC U-P5REMC ----------------------------------- 39 Control unit BX-UCB -------------------------------------- 39 Hand switch U-HSTR2 ------------------------------------ 39 Control box for motorized nosepiece and BF/DF illuminator BX-REMCB ---------------------------------- 39 AC adapter for BX-REMCB U-ACAD4515 -------------------------------- 39 Active auto focus unit U-AFA2M-VIS -------------------------------- 40 Motorized reflected filter wheel U-FWR --------------------------------------- 40 Motorized illumination with power focus BXFMA-F ------------------------------------ 41

NEW

NEW

OBSERVATION TUBES -------------------------------------------------------- 29 30 Widefield trinocular tube U-TR30-2 ------------------------------------ 29 Widefield trinocular tube for IR U-TR30IR ------------------------------------ 29 Widefield erect image trinocular tube U-ETR-4 ------------------------------------- 29 Single port tube with lens U-TLU ---------------------------------------- 29 Single port tube with lens for IR U-TLUIR ------------------------------------- 29 Super widefield trinocular tube U-SWTR-3 ----------------------------------- 30 Super widefield erect image trinocular tube U-SWETR ------------------------------------ 30 Super widefield erect image tilting trinocular tube MX-SWETTR --------------------------------- 30

NEW

DEEP ULTRAVIOLET OBSERVATION SYSTEM -------------------------------- 42 UV248 compatible intermediate tube U-UVF248IM --------------------------------- 42 UV quartz light guide U-UVF2FV/5FB ------------------------------- 42 UV248 compatible light source box + Mercury Xenon lamp housing U-UVF248LB+U-LH80HBXE ----------------- 42

INTERMEDIATE TUBES & ACCESSORIES ------------------------------- 31 32 Magnification changer U-CA ----------------------------------------- 31 Magnification changer 2x U-ECA --------------------------------------- 31 Trinocular intermediate attachment U-TRU --------------------------------------- 31 Dual port U-DP ----------------------------------------- 32 Dual port 1x U-DP1xC ------------------------------------- 32 Eyepoint adjuster U-EPA2 -------------------------------------- 32 Arrow pointer U-APT --------------------------------------- 32

OPTICAL TERMINOLOGY ---------------------------------------------------- 43 47

WELCOME TO UIS2/UIS OPTICS

UIS2/UIS: The System That Maximizes The Advantage Of Infinity-Corrected Optics

What's infinity-corrected optics? UIS2/UIS optics is an infinity-corrected optical system in other words, a system in which light passes from the specimen through the objective lens without forming an image along the way. Instead, it travels in the form of infinity parallel rays to the tube lens. The tube lens is where the intermediate image is formed, whereas in finitecorrected optics, this is done by the objective lens.
Figure 1 Infinity-corrected and finite-corrected optical system principles
Infinity-corrected optical system Parallel light beam Eyepiece

light between the objective lens and tube lens, allowing the creation of user-specific or taskspecific optical systems. To establish real flexibility with such a system, it is necessary to eliminate the occurrence of coma aberration.
*In UIS2/UIS objective lenses, the parfocal distance is designed at 45mm and the focal length of the tube lens is 180mm.
Figure 2 Advantages of Infinity-corrected optical system
Infinity-corrected optical system Finite-corrected optical system

Objective lens

Tube lens

Objective lens

UIS/UIS2 objective lens

Tube lens

Intermediate image Eyepiece

Basic dimensions of UIS2/UIS optical system The UIS2/UIS optical system optimally corrects aberration with a dedicated telan lens and an eyepiece so that the coma aberration and flatness are not degraded even when the telan lens exit pupil position is changed by changing the objective lens and telan distance. This makes it possible to use a distance of 50mm to 170mm from objective lens mounting position to the single port tube with lens.
*Coma aberration: refer to the optical terminology at the end of this document.
Figure 3 Basic dimensions of UIS2/UIS2 optical system
U-TLU (Single port tube with lens) Objective lens Image

Finite-corrected optical system

Objective lens

Intermediate image

Advantages of infinity-corrected optics This system, known as "infinity-corrected optics", offers a number of advantages: There is no change in magnification even when the distance between the objective lens and tube lens is altered. With the total magnification remaining constant, there is no image aberration even when prisms or sliders are interposed between the objective lens and the tube lens. As thousands of users have found by experience, these advantages are crucial to composing the ideal microscope optical system. What's more, it is even possible to freely insert or remove intermediate attachments in the parallel rays of
3

* 40mm 45mm

* 84mm 57.6mm 102mm

Recommended distance 50-170mm

*Basic dimensions when our revolving nosepiece and illuminator are combined. When the position of the illuminator above is changed, illumination performance cannot be maintained.

WELCOME TO UIS2/UIS OPTICS

Features of UIS2 objective lenses UIS2 objective lenses ensure compatibility (screw diameter, optical performance) with the UIS optical system and have the following features compared to conventional objective lenses. 1. Wavefront aberration control The Olympus UIS2 objective lenses set a new standard, with wavefront aberration control in addition to common performance standards of N.A. and W.D. Olympus challenges farther highest order optics which has not been fulfilled by the conventional standards. We offer excellent performance objective lenses by minimizing the aberrations that lower resolution.
*Wave front aberration: refer to the optical terminology at the end of this document.

4. Lightening Weight has been reduced to approximately 2/3 that of conventional products by using an aluminum objective lens barrel cover. This has the effect of lightening the load on the devices at objective lens up/down, suppressing vibrations by lowering the inertial force at objective lens switching, etc. (MPLFLN series, LMPLFLN series) 5. Adoption of eco-lens The glass materials of UIS2 objective lenses are all lead- and cadmium-free eco-glass.

2. Objective lenses with excellent image parcentricity High power SemiApochromatic UIS2 objective lenses make the centration tolerance between objective lenses on the microscope nosepiece keep the image within the enter of the field of view even with digital cameras. (50x or higher power in both MPLFLN and LMPLFLN series) 3. Improvement of color reproducibility UIS2 objective lenses realize natural color reproduction without any chromatic shifts using stringently selected high transmittance glass and advanced coating technology that provides high transmittance which is flat over an ultrawide band wavelength. In addition, since the total optical system, including the tube lens is designed to reproduce a natural color, clear images faithful to the specimen are obtained even with digital imaging.

Based on our conviction that the UIS2/UIS system is the best way to maximize the advantages of infinity-corrected optical systems, we confidently recommend the UIS2/UIS-featured Olympus microscope units for all your high-precision needs in research, inspection and production equipment.
* Refer to the Olympus home page for detailed objective lenses specifications.

SYSTEM DIAGRAM
BXFM SYSTEM DIAGRAM
Video system
Video camera C-mount Video camera B mount 2/3" Video camera S mount 2/3" Video camera F mount U-FMT U-CMAD3 U-BMAD U-SMAD U-TMAD

Refer to pages 36-37

U-TV0.25xC

U-TV0.35xC-2

U-TV0.5xC-3

U-TV0.63xC

U-TV1x-2

Observation/single tubes and eyepieces

WHN Eyepieces Widefield trinocular observation tubes SWH Eyepieces Super widefield trinocular observation tubes

Refer to pages 29-30

U-TLU U-TLUIR

Illumination systems and power supply

U-25ND6, U-25ND25, U-25LBD, U-25IF550, U-25L42, U-25FR, U-BP1100IR, U-BP1200IR

U-AN360-3 U-AN360IR

U-AN

U-PO3 U-POIR

U-POTP3

Revolving nosepiece (Refer to pages 34-35)

Refer to pages 23-28

FS AS
5 6 SHUTTER

FS

AS

ND

BX-RLA2

BX-URA2

U-KMAS

BX-KMA/ BX-KMA-ESD

SZX-TLGAD

U-RCV

LG-SF LG-PS2

U-LGAD

Focusing units

Refer to pages 19-21

BXFM-ILHS

U-DULHA U-LH100-3 U-LH100L-3 U-LH100IR TH4-100 TH4-HS

BXFM-ILH

U-LH100HGAPO U-LH100HG BXFM-ILHSPU U-LH75XEAPO BXFM-F

Stands

Refer to page 22

SZ-STL

U-ST

SZ2-STU2

*Different types may be offered in each area.

SYSTEM DIAGRAM
MOTORIZED UNIT SYSTEM DIAGRAM
Observation tubes (Refer to pages 29-30) MX-AFDIC
U-AN360-3

Refer to pages 38-40

U-AN

U-AN

Intermediate tubes (Refer to pages 31-32)

U-PO3

U-FWO

Refer to page 26
U-AFA2M-VIS U-LH75XEAPO U-LH100HGAPO U-LH100HG

U-POTP3

U-25ND6, U-25ND25 U-25LBD U-25IF550 U-25L42 U-25FR

Mirror units
U-FWR BX-RFAA BX-RLAA

U-LH100-3 U-LH100L-3

U-ZPCB Z board BX-UCB*

U-DICR U-D6REMC U-DICRH

Refer to page 35

Refer to pages 7-18

See manual

BF objective lenses BD-M-AD

U-HSTR2

U-DICRHC

U-D5BDREMC U-D6BDREMC BF/DF objective lenses

PC

analySIS FIVE Control software * BX-REMCB is also available for BX-RLAA + motorized revolving nosepiece control (refer to page 39)

BXFM-A SYSTEM DIAGRAM

Refer to page 41 Refer to page 26
32filter Video system (Refer to pages 37-38) Observation tubes (Refer to pages 29-30) MX-AFDIC U-AN
Auxiliary lens (provided with the BXFMA-F) U-LH100HG U-LH75XEAPO Power source

Refer to pages 27-28

U-AFA2M-VIS LG-SF U-LGAD LG-PS2

BXFMA-F

Refer to pages 26-27

U-FWR

U-LH100-3 U-LH100L-3

U-RMT

BX-UCB

U-ZPCB Z board
See manual

U-D6REMC U-P5REMC U-D5BDREMC U-D6BDREMC U-P6BDREMC

U-HSTR2 U-IFFH PC

Objective lenses

U-FH

analySIS FIVE Control software

UIS2/UIS OBJECTIVE LENSES

Meaning of abbreviations shown on objective lens

M P L (Plan) F L N - 1 0 0

B D

M: LM:

Metallurgical (no cover) Long working distance metallurgical use SLM: Super long working distance metallurgical use LC: Observation through substrate

PL: Plan/ Corrects field curvature of the periphery of the image plane

None: Achromat/ Corrects aberration at 2 wavelengths of blue and red FL: SemiApochromat/ Corrects chromatic aberration in the visible range (violet~red) APO: Apochromat/ Optimally corrects chromatic aberration in the entire visible band (violet~red)

None: UIS N: UIS2

Number: Objective lens magnification

None: Brightfield BD: Brightfield/darkfield BDP: Brightfield/darkfield/ polarizing IR: IR LCD: LCD

Objective lens notation

Objective lenses series abbreviation (PL: Plan) Magnification N.A. (Numerical Aperture)

Infinity-corrected optical system For brightfield observation Cover glass thickness (no cover) Field Number

Objective lens series list

UIS2 Series MPLAPON MPLFLN Magnification 50/100 1.25/2.5 5/10/20/50/100 5/10/20/50/100 5/10/20/50/100 20/50/100 20/50/100 5/10/20/50/100 *4 5/10/20/50/100/150 5/10/20/50/100 5/10/20/50/100 5/10/20/50/100 BF \ \ \ \ \ \ \ \ \ \ \ \ \U \L \L \ \ \*2 \ DF DIC*1 \U POL \ FL F.N. (Field Number) 26.5 1.25x: 22 /2.5x: 26.5 26.5 26.5 22 26.5 26.5 22 26.5 26.5 26.5 22 Remarks Use together with polarizer and analyzer recommended

UIS UIS2

LMPLFLN MPLN LCPLFLN-LCD SLMPLN LMPlanIR/MPlanIR MPLFLN-BD MPLFLN-BDP LMPLFLN-BD MPLN-BD

For LCD For near-IR observation

\ \ \ \

\U aU \L

\ a \

\*2 \*2 \

*1 DIC prism U-DICR: UM/LM position, U-DICRHC: LM position fixed, U-DICRH: UM position fixed. *2 5~20x: U excitation also possible *3 50x: DIC observation not applicable *4 MPlanIR: available 100x only \: Responds a: Optimally responds BF: Brightfield DF: Darkfield DIC: Differential Interference Contrast POL: Polarized light FL: Fluorescence

Features of objective lens series

a MPLAPON: Plan Apochromat P 8 Plan Apochromat objective lenses that correct chromatic aberrations at the highest level. Olympus guarantees*1 optical performance (wavefront aberration) with a Strehl ratio*2 of 95% or better. They are also designed for use with Olympus' U-AFA2M active AF unit. *1 Definition of guaranteed values: Measurements assessed with Olympus Interferometer for Transmitted Wavefront Measurement under specified conditions (measurement: temp. 23C1C; assessment: measurement within the 97% range of the pupil dia.). 2 * Strehl ratio: Indicates in percent (%) the ratio of the proportion of light that an actual optical system can concentrate with respect to the proportion of light concentrated in the image plane (central intensity) by an ideal, aberration-free optical system, with the latter serving as 100%. A higher percentage indicates a higher quality optical system. a MPLFLN series: M Plan SemiApochromat P 9 Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 7 objective lenses ranging from 1.25x to 100x, and secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the reflected light illuminator. a LMPLFLN series: Long WD M Plan SemiApochromat P 10 Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. a MPLN series: M Plan Achromat P 11 Plan Achromat objective lenses providing excellent image flatness up to F.N. 22. a LCPLFLN-LCD series: LCD Long WD M Plan SemiApochromat P 12 Perfect objective lens series for observation of LCD panels and other samples through a glass substrate. Aberration correction matched to the glass thickness is accomplished using a correction ring. a SLMPLN series: Super Long WD M Plan Achromat P 13 Plan Achromat objective lenses with high magnification and super long working distance. Three magnifications, 20x, 50x and 100x are available. For 5x or 10x objective lenses, select from the LMPLFLN Series. a LMPlan-IR series: IR Long WD M Plan Achromat P 14 MPlan-IR: IR M Plan Achromat P 14 IR objective lenses which compensate for aberrations from visible to near infrared light. Ideal for the observations of semiconductor interiors and the back surface of a chip package as well as CSP bump inspection. a MPLFLN-BD series: M Plan SemiApochromat BD P 15 Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-150x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. a MPLFLN-BDP series: M Plan SemiApochromat BDP P 16 Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. The BDP series optimizing brightfield/darkfield and polarized light characteristics is perfect for Nomarski DIC and polarized light observations. a LMPLFLN-BD series: Long WD M Plan SemiApochromat BD P 17 Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. a MPLN-BD series: M Plan Achromat BD P 18 Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.

UIS2 OBJECTIVE LENSES

Plan Apochromat
MPLAPON series
Plan Apochromat objective lenses that correct chromatic aberrations at the highest level. Olympus guarantees*1 optical performance (wavefront aberration) with a Strehl ratio*2 of 95% or better. They are also designed for use with Olympus' U-AFA2M active AF unit.

MPLAPON50x
20.32 4.5 4.5

MPLAPON100x
20.32

8.8 WD=0.35 18.9 20.3 28.5 WD=0.35

5.5 16 20.2 28

*1 Definition of guaranteed values: Measurements assessed with Olympus Interferometer for Transmitted Wavefront Measurement under specified conditions (measurement: temp. 23C1C; assessment: measurement within the 97% range of the pupil dia.). 2 * Strehl ratio: Indicates in percent (%) the ratio of the proportion of light that an actual optical system can concentrate with respect to the proportion of light concentrated in the image plane (central intensity) by an ideal, aberration-free optical system, with the latter serving as 100%. A higher percentage indicates a higher quality optical system.

41.62

37.98

(44.65)

(44.65)

42.78

42.4

45

41

45

38.43

Unit: mm

UIS2 objective lenses Objective lens (magnification) MPLAPON 50x MPLAPON 100x Numerical Aperture 0.95 0.95 Working distance Focal distance f (mm) (mm) 0.35 0.35 3.6 1.8 Weight (g) 139 125

Widefield eyepiece WHN10x Field Number 22

Super widefield eyepiece SWH10x Field Number 26.5

Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (m) magnifications of view (mm) focus (m) 500 1000 0.44 0.22 1.0 0.67 500 1000 0.53 0.27 1.0 0.7

Screw: W20.32x0.706 (0.8"x1/36")

UIS2 OBJECTIVE LENSES

M Plan SemiApochromat
MPLFLN series
Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 7 objective lenses ranging from 1.25x to 100x, and secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the reflected light illuminator.

MPLFLN1.25x*
28 20.32

MPLFLN2.5x*
28 20.32

MPLFLN5x

MPLFLN10x

20.32

20.32

4.5

4.9

4.9

22.55

(25)

4.5

(34.3)

31.6

(41.5)

41.2

45

45

45

WD=3.5

WD=10.7

28.6 29 30

24.5 WD=20 29 30

20.9 WD=11 26

45

(34)

28.41

14.5 26

MPLFLN20x
20.32

MPLFLN50x
20.32

MPLFLN100x
20.32

4.5

4.8

35.1

36.8

4.8

41.78

40.8

(41.9)

42.61

(44)

12.1 WD=3.1 WD=1 26

10.7 WD=1 15.2 17.8 26

(44)

45

45

45

42.4

38.4

7.8 15.2 17.8 26

Unit: mm

UIS2 objective lenses Objective lens (magnification) MPLFLN MPLFLN MPLFLN MPLFLN MPLFLN MPLFLN MPLFLN 1.25x* 2.5x* 5x 10x 20x 50x 100x Numerical Aperture 0.04 0.08 0.15 0.30 0.45 0.80 0.90 Working distance Focal distance f (mm) (mm) 3.5 10.7 20.0 11.0 3.1 1.0 1.0 145 72 36 18 9 3.6 1.8 Weight (g) 122 106 51.5 68.1 70.4 89.9 90.9

Widefield eyepiece WHN10x Field Number 22

Super widefield eyepiece SWH10x Field Number 26.5

Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (m) magnifications of view (mm) focus (m) 12.5 25 50 100 200 500 1000 17.6 8.8 4.4 2.2 1.1 0.44 0.22 870 220 59 15 5.2 1.3 0.73 25 50 100 200 500 1000 10.6 5.3 2.7 1.3 0.53 0.27 220 59 15 5.1 1.3 0.73

Screw: W20.32x0.706 (0.8"x1/36")

UIS2 OBJECTIVE LENSES

Long WD M Plan SemiApochromat

LMPLFLN series

(WD: Working Distance)

Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification.

LMPLFLN5x
20.32

LMPLFLN10x
20.32

LMPLFLN20x
20.32

4.9

4.5

(22.5)

22.2

(24)

22.1

4.8

45

45

WD=22.5

25.4 WD=21 26

45

(33)

29.31

15.2 WD=12 22.4 26

17 26

LMPLFLN50x
20.32

LMPLFLN100x
20.32

4.9

(34.4)

30.4

4.7

(41.6)

45

45

41.1

37.26

WD=10.6

18.2 26 WD=3.4

12.5 15 18.1 26

Unit: mm

UIS2 objective lenses Objective lens (magnification) LMPLFLN LMPLFLN LMPLFLN LMPLFLN LMPLFLN 5x 10x 20x 50x 100x Numerical Aperture 0.13 0.25 0.40 0.50 0.80 Working distance Focal distance f (mm) (mm) 22.5 21.0 12.0 10.6 3.4 36 18 9 3.6 1.8 Weight (g) 50 54 73 77 94

Widefield eyepiece WHN10x Field Number 22

Super widefield eyepiece SWH10x Field Number 26.5

Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (m) magnifications of view (mm) focus (m) 50 100 200 500 1000 4.4 2.2 1.1 0.44 0.22 70 18 6.1 2.5 0.87 50 100 200 500 1000 5.3 2.7 1.3 0.53 0.27 70 18 6.1 2.5 0.87

Screw: W20.32x0.706 (0.8"x1/36")

10

UIS2 OBJECTIVE LENSES

M Plan Achromat
MPLN series
Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.

MPLN5x
20.32

MPLN10x
20.32

MPLN20x
20.32

4.5

(25)

23.4

4.5

(34.4)

33.6

32.71

28.8

4.5

WD=20

WD=10.6

WD=1.3

21 24

10.5 12 16 24

(43.7)

42.4

45

45

45

41.3

37.2

6 11.9 15.8 24

MPLN50x
20.32

MPLN100x
20.32

4.5

42.62

(44.62)

41.3

37.2

4.5

(44.79)

43.16

42.8

45

45

38.7

WD=0.38

11.9 15.8 24

WD=0.21

4.4 11.6 15.6 24

Unit: mm

UIS2 objective lenses Objective lens (magnification) MPLN 5x MPLN 10x MPLN 20x MPLN 50x MPLN 100x Numerical Aperture 0.10 0.25 0.40 0.75 0.90 Working distance (mm) 20.0 10.6 1.3 0.38 0.21 Focal distance f (mm) 36 18 9 3.6 1.8 Weight (g) 64 80 111 113 116

Widefield eyepiece WHN10x Field Number 22 Total Practical field of magnifications view (mm) 50 4.4 100 2.2 200 1.1 500 0.44 1000 0.22 Depth of focus (m) 98 18 6.1 1.4 0.73

Screw: W20.32x0.706 (0.8"x1/36")

11

UIS2 OBJECTIVE LENSES

LCD Long WD M Plan SemiApochromat

LCPLFLN-LCD series
Perfect objective lens series for observation of LCD panels and other samples through a glass substrate. Aberration correction matched to the glass thickness is accomplished using a correction ring.

LCPLFLN20xLCD***
20.32

LCPLFLN50xLCD***
20.32

LCPLFLN100xLCD***
20.32

4.5

20

4.8

20

4.5

24.5

(36.738)

36.55

34.75

45.238 *

45.238 *

45.238 *

(42.038)

(43.638)

40.45

38.65

t=0.7

t=0.7

WD=7.8

25 29.5 31

WD=2.5

17.84 25 29.5 31

WD=0.9

25 29.5 31

t=0.7

15

12.77

43.4

41.6

27.5

25

20

15.2

Unit: mm

* Value at glass thickness 0.7mm observation

Objective lens Corresponding glass thickness (mm) Correction ring indication Working distance (mm) Correction system

0 8.3

LCPLFLN20xLCD 0-1.2 0.7 7.8 Correction ring

1.2 7.4

0 3.0

LCPLFLN50xLCD 0-1.2 0.7 2.5 Correction ring

1.2 2.2

0 1.2

LCPLFLN100xLCD 0-0.7 0.5 0.98 Correction ring

0.7 0.9

UIS2 objective lenses Objective lens (magnification) Numerical Aperture Working distance** Focal distance f (mm) (mm)

Widefield eyepiece WHN10x Field Number 22

Super widefield eyepiece SWH10x Field Number 26.5

LCPLFLN 20xLCD*** 0.45 LCPLFLN 50xLCD*** 0.70 LCPLFLN 100xLCD*** 0.85 Screw: W20.32x0.706 (0.8"x1/36")

Total Practical field Depth of Total Practical field Depth of Weight magnifications of view (mm) focus (m) magnifications of view (mm) focus (m) (g) 7.8 9 146 200 1.1 5.2 200 1.3 5.2 2.5 3.6 170 500 0.44 1.6 500 0.53 1.6 0.9 1.8 185 1000 0.22 0.79 1000 0.27 0.79 **The figure shown here is the value when the correction ring indication is 0.7. *** To be available in the beginning of 2007

12

UIS2 OBJECTIVE LENSES

Super Long WD M Plan Achromat

SLMPLN series
Plan Achromat objective lenses with high magnification and super long working distance. Three magnifications, 20x, 50x and 100x are available. For 5x or 10x objective lenses, select from the LMPLFLN series.

SLMPLN20x
20.32 4.9 4.9

SLMPLN50x
20.32 4.9

SLMPLN100x
20.32

19.8

(20)

19.3

24.1

26.3

45

(27)

16.1 25 WD=25 26 WD=18

45

16.9 WD=7.6 17.3 21.4 26

45

(37.4)

37.2

36.2

14.7 23.8 26

Unit: mm

UIS2 objective lenses Objective lens (magnification) Numerical Aperture Working distance Focal distance f (mm) (mm) 25 18 7.6 9 3.6 1.8 Weight (g) 56 74 100

Widefield eyepiece WHN10x Super widefield eyepiece SWH10x Field Number 22 Field Number 26.5 Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (m) magnifications of view (mm) focus (m) 200 500 1000 1.1 0.44 0.22 11.4 4.2 1.3 200 500 1000 1.3 0.53 0.27 11.4 4.2 1.3

SLMPLN 20x 0.25 SLMPLN 50x 0.35 SLMPLN 100x 0.6 Screw: W20.32x0.706 (0.8"x1/36")

13

UIS OBJECTIVE LENSES

IR Long WD M Plan Achromat/IR M Plan Achromat

LMPlan-IR series/MPlan-IR
IR objective lenses which compensate for aberrations from visible to near infrared light. Ideal for the observations of semiconductor interiors and the back surface of a chip package as well as CSP bump inspection.
UNIVERSAL INFINITY SYSTEM

LMPlan5xIR
20.32 4.5 4.5

LMPlan10xIR
20.32 4.5

LMPlan20xIR
20.32

22.5

(26.5) 25 23

(25)

45

45

21 WD=20 WD=8.1 26 WD=18.5 12.4 15 22 26 10.4 18 26

LMPlan50xIR
20.32 4.5 4.7

LMPlan100xIR
20.32 4.5

45

(36.9)

34.3

30.3

MPlan100xIR
20.32

36.9

32.9

36.8

37.5

(41.6)

40.8

(39)

WD=6

12.5 WD=3.4 15.6 18 26

12.5 15 18.12 26 WD=0.3 4.95 12.2 14.8 26

(44.7)

43.4

45

45

45

42.6

37

Unit: mm

UIS objective lenses Objective lens (magnification) LMPlan 5xIR LMPlan 10xIR LMPlan 20xIR LMPlan 50xIR LMPlan 100xIR MPlan 100xIR Numerical Aperture 0.10 0.25 0.40 0.55 0.80 0.95 Working distance (mm) 20.0 18.5 8.1 6.0 3.4 0.3 Focal distance f (mm) 36 18 9 3.6 1.8 1.8 Weight (g) 73 73 110 115 122 130

Widefield eyepiece WHN10x Field Number 22 Total magnifications 50 100 200 500 1000 1000 Practical field of Depth of focus view (mm) (m) 4.4 98 2.2 18 1.1 6.1 0.44 2.2 0.22 0.87 0.22 0.67

Screw: W20.32x0.706 (0.8"x1/36")

14

UIS2 OBJECTIVE LENSES

M Plan SemiApochromat BD
MPLFLN-BD series

(BD:Brightfield/Darkfield)

Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-150x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification.

MPLFLN5xBD
26

MPLFLN10xBD
26

MPLFLN20xBD
26

4.5

31.5

30.25

4.5

4.5

(38.5)

36.5

39.5

45

45

16 WD=6.5 WD=12 22.4 29.5 32

16.8 22 26.2 27 32 WD=3

45

(42)

39

35.97

31

37

34.1

(33)

17 22 27.5 28.5 32

MPLFLN50xBD
26

MPLFLN100xBD
26

MPLFLN150xBD
26

4.5

4.5

41

4.5

(44)

41

(44)

WD=1

WD=1

27.2 32

27.2 32

WD=1

20

20

(44)

45

45

45

41

20 27.2 32

Unit: mm

UIS2 objective lenses Objective lens (magnification) MPLFLN 5xBD MPLFLN 10xBD MPLFLN 20xBD MPLFLN 50xBD MPLFLN 100xBD MPLFLN 150xBD Screw: W26x0.706 Numerical Aperture 0.15 0.30 0.45 0.80 0.90 0.90 Working distance Focal distance f (mm) (mm) 12.0 36 6.5 18 3.0 9 1.0 3.6 1.0 1.8 1.0 1.2 Weight (g) 95.5 82.8 87.7 99.8 98.9 104.8

Widefield eyepiece WHN10x Field Number 22

Super widefield eyepiece SWH10x Field Number 26.5

Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (m) magnifications of view (mm) focus (m) 50 4.4 59 50 5.3 59 100 2.2 15 100 2.7 15 200 1.1 5.2 200 1.3 5.2 500 0.44 1.3 500 0.53 1.3 1000 0.22 0.73 1000 0.27 0.73 1500 0.15 0.6 1500 0.18 0.6

15

UIS2 OBJECTIVE LENSES

M Plan SemiApochromat BDP

MPLFLN-BDP series

(BDP:Brightfield/Darkfield/Polarizing)

Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification. The BDP series optimizing brightfield/darkfield and polarized light characteristics is perfect for Nomarski DIC and polarized light observations

MPLFLN5xBDP
26

MPLFLN10xBDP
26

MPLFLN20xBDP
26

4.5

4.5

(33)

31.5

30.25

4.5

(38.5)

39.5

45

45

45

(42)

39

35.97

31

36.5

37

34.1

16 WD=6.5 WD=12 22.4 29.3 32

16.8 22 26 27 32 WD=3

17 22 27.5 28.5 32

MPLFLN50xBDP
26

MPLFLN100xBDP
26

4.5

4.5

41

(44)

20 WD=1 27.5 32 WD=1

(44)

45

45

41

20 27.5 32

Unit: mm

UIS2 objective lenses Objective lens (magnification) MPLFLN 5xBDP MPLFLN 10xBDP MPLFLN 20xBDP MPLFLN 50xBDP MPLFLN 100xBDP Screw: W26x0.706 Numerical Aperture 0.15 0.25 0.40 0.75 0.90 Working distance Focal distance f (mm) (mm) 12.0 36 6.5 18 3.0 9 1.0 3.6 1.0 1.8 Weight (g) 95.5 83.3 88.5 100.5 101.5

Widefield eyepiece WHN10x Field Number 22

Super widefield eyepiece SWH10x Field Number 26.5

Total Practical field Depth of Total Practical field Depth of magnifications of view (mm) focus (m) magnifications of view (mm) focus (m) 50 4.4 59 50 5.3 59 100 2.2 18 100 2.7 18 200 1.1 6.1 200 1.3 6.1 500 0.44 1.4 500 0.53 1.4 1000 0.22 0.73 1000 0.27 0.73

16

UIS2 OBJECTIVE LENSES

Long WD M Plan SemiApochromat BD

LMPLFLN-BD series
Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. Suitable with samples having a height difference and in preventing collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification.

LMPLFLN5xBD
26

LMPLFLN10xBD
26

LMPLFLN20xBD
26

4.5

26.6

26.2

24

30.2

4.5

32.3

(35)

45

45

45

(33)

32.5

32

32.2

(30)

31.3

16.2 WD=15 WD=10 22.4 28 29.5 32

15.5 21.9 28.2 30 32 WD=12

22 23 28 30 32

LMPLFLN50xBD
26

LMPLFLN100xBD
26

4.8

(34.4)

32.7

31.8

33

4.7

45

20.7 WD=10.6 23 28 32 WD=3.3

45

(41.7)

37.7

37.3

36.5

21 29 30.3 32

Unit: mm

UIS2 objective lenses Objective lens (magnification) LMPLFLN 5xBD LMPLFLN 10xBD LMPLFLN 20xBD LMPLFLN 50xBD LMPLFLN 100xBD Screw: W26x0.706 Numerical Aperture 0.13 0.25 0.40 0.50 0.80 Working distance Focal distance f (mm) (mm) 15.0 36 10.0 18 12.0 9 10.6 3.6 3.3 1.8 Weight (g) 81 84 86 85 102

Widefield eyepiece WHN10x Field Number 22

Super widefield eyepiece SWH10x Field Number 26.5 Depth of focus (m) 70 18 6.1 2.5 0.87

Total Practical field Depth of Total Practical field magnifications of view (mm) focus (m) magnifications of view (mm) 50 4.4 70 50 5.3 100 2.2 18 100 2.7 200 1.1 6.1 200 1.3 500 0.44 2.5 500 0.53 1000 0.22 0.87 1000 0.27

17

UIS2 OBJECTIVE LENSES

M Plan Achromat BD
MPLN-BD series
Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.

MPLN5xBD
26

MPLN10xBD
26 (4.5)

MPLN20xBD
26

4.5

31.5

30.25

4.5

(38.5)

36.5

34.01

(33)

31

37

(43.7)

42.5

45

16 WD=12 29.3 30.5 32 WD=6.5

45

16.8 22 26 27 32 WD=1.3

45

41

17 23.6 29 32

MPLN50xBD
26

MPLN100xBD
26

4.5

4.5

(44.62)

(44.79)

43.68

43.71

43.33

42.5

42.5

41

45

45

41

10 WD=0.38 20.7 23.1 29 32 WD=0.21

10 23 27 29 32

Unit: mm

UIS2 objective lenses Objective lens (magnification) MPLN MPLN MPLN MPLN MPLN 5xBD 10xBD 20xBD 50xBD 100xBD Numerical Aperture 0.10 0.25 0.40 0.75 0.90 Working distance (mm) 12.0 6.5 1.3 0.38 0.21 Focal distance f (mm) 36 18 9 3.6 1.8 Weight (g) 137 155 162 157 160

Widefield eyepiece WHN10x Field Number 22 Total Practical field of magnifications view (mm) 50 100 200 500 1000 4.4 2.2 1.1 0.44 0.22 Depth of focus (m) 98 18 6.1 1.4 0.73

Screw: W26x0.706

18

MICROSCOPE SYSTEM BXFM

BXFM frame
BXFM-F
Widely used system that allows use in combination with fiber illumination, motorized revolving nosepiece and telan lens unit. Can easily be integrated into other equipment. Attach to the equipment by rear bolt mounting screw or pillar mounting hole.

84 36 82 58.5 Stroke 36 (13) 4-M8 depth8 (Bolt mount screw) 35 7 4M4 depth9

23

100

124

17

80

0.5

0.5 66.2 4M4 depth7

2A00002

55

17 Pillar mount hole center

34

3 2H (Pi 8 llar mo
98 110

un

th

ole

Weight: 1.9kg

16

17

(13)

Unit: mm

19

MICROSCOPE SYSTEM BXFM

BXFM
BXFM-F+BXFM-ILH+BXFM-ILHSPU
Accommodates the reflected light brightfield/darkfield and fluorescence illuminators.

180

Light axis 165 130 Light axis 83 Pillar axis Holder mounting position

Revolving nosepiece mounting position 40 72 Objective lens mounting position 45 11

Stroke

23

Specimen position

3.5

Weight: 3.2kg

Unit: mm

BXFM combination sample BXFM-F+BXFM-ILH+BXFM-ILHSPU+TR30-2+BX-RLA2+U-LH100L-3

165 130 87.5 83

32 249

3.5

220

180 587

Specimen surface

* For installation dimensions, refer to those for the BXFM-F (page 19).

45

Weight: 8.2kg (exclude objective lens)

Unit: mm

20

11

17-47(stroke) 124

40

MICROSCOPE SYSTEM BXFM

BXFM-S
BXFM-F+BXFM-ILHS
Compact focusing unit suitable for building into existing equipment.

Pillar axis

141 106 59

Holder mounting position Light axis

84

20

Revolving nosepiece mounting position Objective lens mounting position

40

Stroke 23

45

Weight: 2.4kg
Specimen position

Unit: mm

BXFM-S combination sample BXFM-F+BXFM-ILHS+TR30-2+U-KMAS+U-LH100L-3

106

187

290

32

124

20

19-49 (Stroke)

45

40

169 Specimen surface 84 208

* For installation dimensions, refer to those for the BXFM-F (page 19).

92.5

Weight: 5.5kg (exclude objective lens)

Unit: mm

21

MICROSCOPE SYSTEM BXFM

Stands
A wide variety of stands are available to suit different applications and purposes.

SZ2-STU2 Universal stand type 2

Major specifications
Item 1 Diameter of focusing arm or fixing section of tube 2 Vertical pole diameter 3 Horizontal poles diameters 4 Stroke 5 Movement range
300 350 622 214~435 180

Specifications 32mm 40mm 25mm (both upper and lower poles) Horizontal: 234mm, Vertical: 205mm Horizontal: 541 (435+106) mm max. (Vertical pole BXFM-S optical axis) Forward: 10kg (within 90-degree area) Transverse direction: 6kg Backward direction: 7kg (at maximum stroke) 30kg

250

300

6 Maximum specimen weight

25

30
90

7 Weight

32 503 40 130

* The rotation angle of the horizontal arm can restrict to 90 degrees with stopper.

U-ST Compact stand

50.5

SZ-STL Large stand

133

110

88

400 35 73 32 106 143 32

160

320

133

49

20

Weight: 1.8kg

Weight: 5kg

17.5

45

46

267

Unit: mm

22

ILLUMINATION UNITS

Reflected light illuminator for BF/DF

BX-RLA2
ND filters are linked when exchanging between brightfield and darkfield.
144 31.5

Accessories Unit name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 U-AN360-3 U-AN U-DICR U-DICRH U-DICRHC Description LBD filter slider IF550 filter slider ND filter ND filter Frost filter slider UV-cut filter Polarizer slider for reflected light Polarizer slider for reflected light with tint plate 360 rotatable analyzer slider Analyzer slider for reflected light DIC slider for reflected light DIC slider for reflected light (high resolution type) DIC slider for reflected light (high contrast type) Weight (g) 20 20 20 20 20 20 71 71 79 50 130 130 130
Revolving nosepiece mounting position

46

30

108

75 88

17.5

12

17

26

11.8

29.4

56.8

38.7

84

Illuminator mounting position

41

13

16.2

3.5

45 265 335

Weight: 3.4kg

Universal reflected light illuminator

BX-URA2
Suitable for observations ranging from brightfield to fluorescence. Six mirror units can be attached to this reflected light illuminator simultaneously.
Accessories Unit name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 U-AN360-3 U-AN U-DICR U-DICRH U-DICRHC U-MBF3 U-MDF3* U-MDIC3 U-MBFL3 U-MWUS3 U-MWBS3 U-MWGS3 Description LBD filter slider IF550 filter slider ND filter ND filter Frost filter slider UV-cut filter Polarizer slider for reflected light Polarizer slider for reflected light with tint plate 360 rotatable analyzer slider Analyzer slider for reflected light DIC slider for reflected light DIC slider for reflected light (high resolution type) DIC slider for reflected light (high contrast type) Mirror unit for reflected brightfield Mirror unit for reflected darkfield Mirror unit for reflected DIC Mirror unit for reflected brightfield, for high intensity light source Fluorescence mirror unit for reflected (U excitation) Fluorescence mirror unit for reflected (B excitation) Fluorescence mirror unit for reflected (G excitation) Weight (g) 20 20 20 20 20 20 71 71 79 50 130 130 130 80 80 80 80 80 80 80
Revolving nosepiece mounting position 84

(152)

76

17

11.6

86.8

11.6

3.5

41

Illuminator mounting position

13

12

27.2

41 261 367

* U-RCV (DF converter for BX-URA2) is needed with darkfield observation.

84 88

Weight: 3.8kg

Unit: mm

23

35 7.5

107.5

30

(17

.9

ILLUMINATION UNITS

Reflected light illuminators for BF

BX-KMA/BX-KMA-ESD
Enables brightfield, Nomarski DIC and simple polarizing observations. ESD model is also available.

Accessories Unit name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 U-AN360-3 U-AN U-DICR U-DICRH U-DICRHC Description LBD filter slider IF550 filter slider ND filter ND filter Frost filter slider UV-cut filter Polarizer slider for reflected light Polarizer slider for reflected light with tint plate 360 rotatable analyzer slider Analyzer slider for reflected light DIC slider for reflected light DIC slider for reflected light (high resolution type) DIC slider for reflected light (high contrast type) Weight (g) 20 20 20 20 20 20 71 71 79 50 130 130 130
108 70 30 250 312.5 88

Revolving nosepiece mounting position Illuminator mounting position

84

41

3.5

Cable length 260.5mm

* Combine SZX-TLGAD when using fiber illumination.

Weight: 3.1kg

Unit: mm

MOUNTING DIMENSIONS OF ILLUMINATORS (BX-RLA2, BX-URA2 and BX-KMA/BX-KMA-ESD)

21 170 (Distance to the light axis)

100 (0.1) 36 (0.1)

5.5 4-M5 depth12 or more

52 (Revolving nosepiece relief dimension)

3.5

Location face (11)

37 0.1 (45 location face)

45

(10

2.5

')

Location face

82 (Revolving nosepiece relief dimension) Convex section(2-4) for positioning

Fix illuminator using four M5 screws and projection for fastening.

(6)

62 (0.1)

Le ss th an R6

Unit: mm

24

LAMP HOUSING & ACCESSORIES

Reflected light illuminator for BF

U-KMAS
Very compact reflected light illuminator with reduced depth.

Accessories Unit name U-25LBD U-25IF550 U-25ND6 U-25ND25 U-25FR U-25L42 U-PO3 U-POTP3 U-AN360-3 U-AN U-DICR U-DICRH U-DICRHC Description LBD filter slider IF550 filter slider ND filter ND filter Frost filter slider UV-cut filter Polarizer slider for reflected light Polarizer slider for reflected light with tint plate 360 rotatable analyzer slider Analyzer slider for reflected light DIC slider for reflected light DIC slider for reflected light (high resolution type) DIC slider for reflected light (high contrast type) Weight (g) 20 20 20 20 20 20 71 71 79 50 130 130 130
64

21

88

155 198

21

75

Weight: 1.2kg

Unit: mm

25

LAMP HOUSING & ACCESSORIES

Lamp housings
Various different lamp housings are available, for use with different light sources: choose to suit the intended purpose.
* If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand.

U-LH75XEAPO 75W xenon apo lamp housing

U-LH100HGAPO 100W mercury apo lamp housing U-LH100HG 100W mercury lamp housing

(148.5) 65 83.5 65

30.2 93 65 8

(148.5) 83.5 65

(30.2) 93

30

30

25

8 115 115 169 (depth dimension for installation) 180.5

25

40.8

6 20

55

75

20

40.8

130

70

169 (depth dimension for installation) 180.5 130 18.5

Cable length 2,000mm

Accepted lamp: UXL-75XB

Weight: 3.1kg

Cable length 2,000mm

Accepted lamp: USH-103OL

Weight: 2.7kg

*Power supply unit (U-RX-T200) and power cable (UYCP) are necessary for 75W xenon lamp housing. These items are sold separately. U-RX-T200 dimensions: 115(W)x195(D)x260(H), weight approx. 3kg

* Power supply unit (U-RFL-T200) and power cable (UYCP) are necessary for 100W mercury lamp housings. These items are sold separately. U-RFL-T200 dimensions: 150(W)x295(D)x200(H), weight approx. 4.8kg

U-LH100-3/U-LH100IR/U-LH100L-3 100W halogen lamp housings

107

Cable length U-LH100-3: 290mm U-LH100IR: 290mm U-LH100L-3: 800mm

85.5

Weight: 880g

Accepted lamp: 12V100WHAL (high intensity lamp) 12V100WHAL-L (long life lamp)

135(depth dimension for installation) 146.5

* External power supply (TH4-100 or TH4-200) and power cable (UYCP) are necessary for 100W halogen lamp housings. These items are sold separately. For TH4-100/200 installation dimensions, refer to the next page. Unit: mm

26

37

75

LAMP HOUSING & ACCESSORIES

Lamp housing accessories

For the 100W halogen lamp, the external power supply TH4-100/200 with an intensity adjustment switch and an ON/OFF switch, both are located close to the operator's hand, are provided. All Olympus reflected light illuminators can be used with fiber illumination.
* If you use the units in your production line, please consult your nearest Olympus representative in your region about the use conditions beforehand.

TH4-100/200 External power supply

TH4-HS Hand switch

Cable length: 2,000mm 18.5

125

120

Weight: 2.2kg
42 75 14.5 200 38

77

Weight: 140g

U-RMT Extension cord

1,700

Weight:200 g

U-RCV DF converter for BX-URA2

U-LGAD Fiber adapter for reflected light observation

SZX-TLGAD Transmitted light guide adapter

75

67

32

59

59

30

12 24 44

37.5

21 31.5 (42.5) (dimensions for installation) 51 62.5 (dimension for installation)

26

(dimension for installation)

Darkfield light excluding tube which is built into the BX-URA2.

* Mountable with BX-KMA/BX-KMA-ESD only.

Weight: 315g

Light guide mount hole 12 Weight: 390g

Light guide mount hole 12 Weight: 135g

Unit: mm

27

49

43

LAMP HOUSING & ACCESSORIES

LG-PS2* Light source

15 (Light guide mounting position)
76 8 235

86

130

10 10

126

251

*The types of model varies by country in use. Weight: 1.6kg

LG-SF Light guide

10 Groove: Width3, Depth1

15

10.1

25

31 61

30

13

25

20

1,000

Weight: 210g

U-DULHA Double lamp house adapter

82 202

12

14

0
171

Weight: 1.2kg

88

Unit: mm

28

OBSERVATION TUBES

Widefield trinocular observation tubes

Trinocular observation tubes with widefield of view. Compatible with F.N. 22.

U-TR30-2/ Widefield trinocular tube U-TR30IR Widefield trinocular tube for IR

U-ETR-4 Widefield erect image trinocular tube

175

59.65

92.5* (IR: 93.9)

18

47.9 62.5 (IR: 64.5)

95.8

150.5 186.6 (IR: 188.9)

60.6

43.5

163.1 199.9

51.6

62.5 103.9 104.9

92

33.5

16

120

Unit: mm

Name U-TR30-2 U-TR30IR U-ETR-4

Field Number (F.N.) 22 22 22

Inclination angle (degree) 30 30 30

Interpupillary distance (mm) 50-76 50-76 50-76

Light path selector (eyepiece/video port) 100/0, 20/80, 0/100 100/0, 0/100 100/0, 0/100

Observation image Inverted Inverted Erect

Weight (g) 1600 1600 1900

*Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62mm.

Single port tube with lens

When the visual observation is not needed and only video observation is required, a single port tube with a built-in telan lens can be attached directly to the video port.
U-TLU Single port tube with lens U-TLUIR Single port tube with lens for IR
60

56

Weight: 350g For attachable video camera adapters, refer to video camera adapters system diagram page (pages 5-6).

Unit: mm

29

OBSERVATION TUBES

Super widefield trinocular observation tubes

Trinocular observation tubes with super widefield of view. Compatible with F.N. 26.5.

U-SWTR-3 Super widefield trinocular tube

U-SWETR Super widefield erect image trinocular tube

180.8

14

63.1

82.3*

173.8 220.8

68.6

49.9 201.9 248.9

55.6 79.6 98.8

76.4*

98.6

62

MX-SWETTR Super widefield erect image tilting trinocular tube

117.223

318.527

42

0' 0 "

20 0' 0"

149.081

70.330

2.837

328.338 337.718

72.200

88.000 95.929 101.929

139.400

61.981

Name U-SWTR-3 U-SWETR MX-SWETTR

Field Number (F.N.) 26.5 26.5 26.5

Inclination angle (degree) 24 24 0-42

Interpupillary distance (mm) 50-76 50-76 50-76

Light path selector (eyepiece/video port) 100/0, 20/80, 0/100 100/0, 0/100 100/0, 0/100

Observation image Inverted Erect Erect

Weight (g) 2300 4200 4200

*Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62mm.

30

73 83.4 92.9

36.5

19

224

Unit: mm

INTERMEDIATE TUBES & ACCESSORIES

Intermediate tubes
Various accessories for various observation need.

U-CA Magnification changer Provides 1x, 1.2x, 1.6x and 2x intermediate magnifications.
138

U-ECA Magnification changer 2x Provides 1x and 2x intermediate magnifications.

88
140

96 75

42

42 45

Weight: 1.3kg
70 52

37

150

Weight: 1.3kg

U-TRU Trinocular intermediate attachment Intermediate attachment which divides the light path, allowing attachment of both digital and video cameras.
183.9
140

37

150

106.9

52

BI:PT=100:0/20:80

58.2

Weight: 1.3kg

Unit: mm

31

INTERMEDIATE TUBES & ACCESSORIES

U-DP Dual port Use this intermediate tube to divide the light path.
88
140

U-DP1xC Dual port 1x Combine with U-DP to obtain a 1x image.

17.53

44 25

30 4.5 170.5(mount face) 182

151

1-32UN

Weight: 500g
51(mount face) 38

57

Weight: 1kg

Light path selector by mirror unit Transmitted side port: side port = 100:0 Transmitted side port: side port = 70:30 (with use of U-MBF3)

U-APT Arrow pointer Projects an arrow into the field of view.

89

(8)
45
15V0.2A

30

0.92 (tolerence from light axis) 120 45.3

Weight: 1.2kg Weight: approximately 500g Unit: mm

32

(45)

88

21.2

115

U-EPA2 Eyepoint adjuster Raises eyepoint by 30mm.

44

EYEPIECES/FILAR MICROMETER EYEPIECE

Eyepieces
Eyepieces for UIS2 optical system.

WHN10x Widefield eyepiece

WHN10x-H CROSSWHN10x Widefield eyepieces

WH15x Widefield eyepiece

SWH10x-H MICROSWH10x CROSSSWH10x Super widefield eyepieces

46.2

41 38.5

41 38.5

41 36.5 EP

43.2 39 EP

48.6

48.6

29.6

39.6

39.4

18.7

36.8

27.8

53.2

28

51.2

62.6

(23.1)

(23.2)

(25) 43.7

41.4 30 (28.5)

30

30

30

Name WHN10x WHN10x-H CROSSWHN10x WH15x SWH10x-H MICROSWH10x CROSSSWH10x *EP=eyepoint

Field Number 22 22 22 14 26.5 26.5 26.5

Diopter adjustment range (1/m) -8 +5 -8 +5 -8 +2 -8 +2 -8 +2

Weight Micrometer diameter (mm) (g) 24 24 24 90 170 170 90 210 210 210

Remarks

With adjustable diopter With cross lines and adjustable diopter With adjustable diopter With micrometer and adjustable diopter With cross lines and adjustable diopter

Filar micrometer eyepiece

U-OSM
Used for precise measurement in the field of view.

137 129.2 117.8

23.5

Eyepiece

Magnification 10, erect image (inverted when used with erect image observation tube), F.N. 14. Diopter adjustment range: 5 1/m. Provided with rubber eye shade.

EP

Measuring scale Scale lines graduated in increments of 1mm in the entire 10mm length. Shift of scale lines: 1mm per rotation of the shift ring, the circumference of which is divided into 100 graduations. Measuring range 10mm/objective lens magnification

65 41

30

Mounting position (inside)

Compensation limit for objective lens magnification tolerance Actual size

5% by combined use of the zoom compensation ring and the provided stage micrometer. Compensation ring clamping screw. Magnification compensation scale. Actual size (mm) = Measured value (mm) Objective lens magnification

29.5

60.9

75.5

Repeatability

0.007 mm A (A Objective lens magnification) Repeatability error *Measuring error (A Objective lens magnification: L Measured length in mm) 0.007 [ (0.0002A+0.002) L + ] mm A Unit: mm

Weight: 580g

Accuracy

33

69.9

Unit: mm

60.2

REVOLVING NOSEPIECES

Revolving nosepieces for BF objective lenses

Choose from following 6 types. For motorized nosepieces, refer to motorized unit page.

U-5RE-2 Quintuple revolving nosepiece

U-D6RE Sextuple revolving nosepiece with slider slot for DIC U-D6RE-ESD Sextuple revolving nosepiece with slider slot for DIC with ESD treatment

U-D7RE Septuple revolving nosepiece with slider slot for DIC

(87.4) 26.5 60.9 38 (114.4) 76.4 38

(125.6) 87.6

40.8

40

48.2

40

102
84

.4
116.5

Weight: 520g

Weight: 800g

47.2

Weight: 980g

U-P4RE Centerable quadruple revolving nosepiece with slider slot for DIC

U-P6RE Centerable sextuple revolving nosepiece with slider slot for DIC

40

38

(114.4) 76.4

(125.6) 38 87.6

48.2

47.2

40

102

.4

40

116

.5

Weight: 1kg

Weight: 1kg

Insert the DIC dummy when not using the DIC slider

116.5

104

Unit: mm

34

116.5

83

104

REVOLVING NOSEPIECES

Revolving nosepieces for BF/DF objective lenses

Choose from following 3 types. Use of adapter to mount BF objectives (BD-M-AD) enables attachment of brightfield objective lenses. For motorized nosepieces, refer to motorized unit page.

U-5BDRE Quintuple revolving nosepiece for BF/DF

U-D5BDRE Quintuple revolving nosepiece for BF/DF with slider slot for DIC

U-D6BDRE Sextuple revolving nosepiece for BF/DF with slider slot for DIC/ U-P5BDRE Centerable quintuple revolving nosepiece

104

104

(125.6) 34.8 (111.2) 76.4 (114.4) 38 76.4 38 87.6

40

40

48.2

48.2

102

.4

102

.4
116 .5

Weight: 800g

Weight: 800g

47.2

40

Weight: 1kg

Insert the DIC dummy when not using the DIC slider

BD-M-AD Adapter to mount BF objectives

W260.706

W20.320.706

30

28.2

+0.2 0

Weight: 10g

(4)

Unit: mm
8

35

116.5

U-D6BDRE U-D6BDRE

2A00002 JAPAN

U-D6BDRE
JA PA N

VIDEO CAMERA ADAPTERS

C-mount video camera ports

Allows direct attachment of a C-mount video camera. Four types are provided: 0.63x, 0.5x, 0.35x and 0.25x. All models feature a focus adjustment function

U-TV0.25xC C-mount video port with 0.25x lens

U-TV0.35xC-2 C-mount video port with 0.35x lens

17.53

36

3.5

Image plane

1-32UN

Image plane 147.3 156.84 17.53

1-32UN 4

60

60 64

Weight: 1.2kg

U-TV0.5xC-3 C-mount video port with 0.5x lens

U-TV0.63xC C-mount video port with 0.63x lens

30
Image plane 1-32UN

17.53

1-32UN Image plane 17.53 30 3.5

68.75
2 A0 0 0 0 1

3.5
60

60

30.1 32.6 42.1

Weight: 200g

78.25

12.4 22.4

Weight: 100g

Weight: 430g

Unit: mm

Field of view (F.N.) 2/3" CCD 1/2" CCD

]Video camera adapter (Projection lens)

Projection magnifications

Projection area (F.N.)

2/3" CCD 11 17.5 22

1/2" CCD 8 12.7 16 22

1/3" CCD 6 9.5 12 17.1 24

U-TV1x-2 U-TV0.63xC U-TV0.5xC-3 U-TV0.35xC-2 U-TV0.25xC

Practical field of view (mm) =

1x 0.63x 0.5x 0.35x 0.25x

Projection area (Field Number) Objective lens magnifications

Projection area

Focus the video camera adapter to prevent defocusing the eyepiece image and defocusing by magnification switching. Generally, the video camera adapter is focused by switching to a low magnification after focusing at a high magnification.objective lens.

36

VIDEO CAMERA ADAPTERS

Video camera mount adapters

Allows attachment to video cameras with C, Bayonet, Sony and F mounts. Use with the U-TV1x-2. Focus by amount of screwing into U-TV1x-2.

U-CMAD3 C-mount adapter

44.5 30 Image plane 17.53 1-32UN

U-BMAD Bayonet mount adapter

U-SMAD Sony mount adapter

Image plane 4 38

Image plane

48

48

60.5

80.5

42

48.7

49

20

M56X2

M56X2

M56X2

64.4

64

64

Weight: 165g

Weight: 80g

Weight: 90g

U-TMAD T mount adapter

Image plane

U-FMT F/T mount adapter *

Image plane 55 46.5

M42X0.75 4 42

45.7 13

23

43

54.7 M56X2

64

Weight: 70g

Weight: 30g * It must be combined with U-TMAD Unit: mm

Video camera port

This port can be attached directly to the trinocular observation tube as well as to the single port tube with lens.

U-TV1x-2 Video port 1x

64

22

60

Weight: 150g

28

40 60

30

Unit: mm

37

MOTORIZED UNITS

Motorized units
Various motorized units, perfect for automation of equipment, are available.

BX-RLAA+U-D6REMC+U-LH100-3 Motorized BF/DF reflected light illuminator+motorized Nomarski DIC sextuple revolving nosepiece+100W halogen lamp housing Enables motorized exchange of objective lenses, selection between brightfield and darkfield observations as well as aperture diaphragm closing/opening. The BX-UCB control unit has an RS232C connector, allowing control via a PC. For method of attaching illuminator, refer to page 24.

80

115 108

86.2 87

11.8

12

(487) 400

84

40

(169)

64.8

45

Illuminator cable length: 1.800mm

81.5

Weight: 5.5kg(exclude objective lens)

BX-RFAA Motorized universal reflected light illuminator Reflected light fluorescence illuminator with simultaneous attachment of six mirror units. Incorporates motorized mirror unit changeover and shutter.

14

92

84

76

17

86.8

11.6

27.2

126 109.5

Illuminator mounting position

41

Revolving nosepiece mounting position

87.5

11.6

12

88

15

Illuminator cable length: 1.800mm

135 261 371 41

211.5

41

107

Weight: 4.3kg

Unit: mm

38

MOTORIZED UNITS

U-D6BDREMC Motorized sextuple BD revolving nosepiece with slider slot for DIC U-P5BDREMC Motorized centerable quintuple BD revolving nosepiece with slider slot for DIC

U-D5BDREMC Motorized quintuple BD revolving nosepiece with slider slot for DIC U-D6REMC Motorized sextuple revolving nosepiece with slider slot for DIC U-P5REMC* Motorized centerable quintuple revolving nosepiece with slider slot for DIC
34

34

14

115.5

14

83.5 103.5 135 150 40.5 103.5 135 150 25.5

74.5

40.5

25.5

40

51.1

40

115

50

46

50 46

69.8

Weight: 1.4 kg

68.3

*Please contact Olympus for the availability. Weight: 1.1kg

BX-UCB Control unit Motorized units including motorized illuminator and auto focus unit can be totally controlled from BX-UCB

U-HSTR2 Hand switch

32

216

212

108

105

7 146

Cable length 2000mm

Weight: 370g

310 125 332 (depth)

Weight: 1.0kg * Extension cord U-RMT (1700mm) should be used to connect the lamp housing (U-LH100-3) to the BX-UCB.

BX-REMCB Control box for motorized nosepiece and BF/DF illuminator BX-RLAA and U-D5BDREMC/U-D6REMC/U-P5REMC can be controlled from U-HSTR2, or direct from the computer keyboard via an RS232C connector. * BX-RFAA and U-D5BDREM/U-D6REM combination not applicable.
39.8 190.4 34

U-ACAD4515 AC adapter for BX-REMCB

2000 +100 0

144

711

129.51 351

Unit: mm

39

50

MOTORIZED UNITS

U-AFA2M-VIS Active auto focus unit Featuring an AF laser light source in wavelength 785 nm. The multiple-spots sensor enables the high-speed and stable focusing of specimens with variable height differences.

313

108

62.5

51

45

Weight: 2.6kg

Cable AFA2M-CBL2M AFA2M-CBL3M

Weight: 0.23kg Weight: 0.36kg

length: 2m length: 3m

* Consult your Olympus dealer about the motorized focus.

U-FWR Motorized reflected filter wheel Accomplish maximum 6 filter position exchange
180.5 147.9

Weight: 1.0kg

2000

24.5 58.5

42

30.5

130

Unit: mm

40

MOTORIZED UNITS

BXFMA-F Motorized illumination with power focus A motorized microscope unit for integration with your equipment. Motorized operations such as revolving nosepiece up/down, objective lens switching, aperture diaphragm open/close, and brightfield/darkfield switching are accomplished with this component. Several microscopic operations are totally controlled from an external unit by combining this component with an auto focus unit. This is the configuration combined with Active Auto Focus Unit U-AFA2M-VIS, Single Port Tube with Lens U-TLU, a lamphousing, a motorized nosepiece and objective lenses.

206

77 45 56 133 169 32.5

Stroke
77 51 7 2 334 342.1 92

36

310.5

Specimen surface (standard focusing position)

* Consult your Olympus dealer about the mounting dimensions.

Weight: 13kg (BXFMA-F frame 7.6kg)

U-FH Focus adjustment knob unit

U-IFFH Focus adjustment knob interface

70 33.6

91.5

50

Weight: 760g
70 75.5 4 54 82.3 210 214

100 104

Weight: 1450g

Unit: mm

41

DEEP ULTRAVIOLET OBSERVATION SYSTEM

Deep ultraviolet observation system

This module adds a deep ultraviolet (248nm) optical system to a general microscope. An ultra-high resolution observation is executed by using an extremely short wavelength ray.

U-UVF248IM UV248 compatible intermediate tube

8 (light guide)

U-UVF2FB/5FB UV quartz light guide

+200 0 +200 0

2000 79

or 5000

108

30.4

34 69

Weight: U-UVF2FB 50g U-UVF5FB 80g

105.8 258.5

39.4 30 1-32 UNF (C Mount Thread)

102.5

196.8 45 (distance between mounting positions) 70 (39.5) 5.5

157.5

196

Weight: 1.9kg

U-UVF248LB+U-LH80HGXE UV248 compatible light source box + Mercury Xenon lamp housing

45 30

20 150 180 170 150

4-4.5, 8 C'bore 5 Deep 4-4.5, 13 C'bore 5 Deep

170

240

41

Weight:6.5kg

227

210.3

Unit: mm

42

OPTICAL TERMINOLOGY

1. Field Number (F.N.) and Practical Field of View The field number (F.N.) is referred to as the diaphragm size of eyepiece in mm unit which defines the image area of specimen. The diaphragm diameter actually seen through eyepiece is known as the practical field of view (F.O.V.) which is determined by the formula: Eyepiece F.N. (mm) Objective lens magnification

5. Total Magnification 5.1 Observation through eyepiece (binocular observation)

M(bino)=M(ob)M(oc) M(bino): Total magnification for binocular observation M(ob): Objective lens magnification M(oc): Eyepiece magnification
5.2 Video monitor observation Total magnification for video monitor

F.O.V. =

M(video monitor)=M(ob)M(video camera adapter)Monitor magnification*

2. Working Distance (W.D.) The distance between the front edge of the objective lens and the specimen surface (with the surface of the cover glass in case of the cover glass objective lens) when the specimen is focused.

M(video monitor): Total magnification on the video monitor M(ob): Objective lens magnification M(video camera adapter): Projected magnification for video camera
adapter including photo eyepiece
(refer to Figure 1) * Refer to Figure 3 for "Monitor magnification"

Practical field of view for video monitor observation 3. Parfocal Distance It is the distance between the objective lens mounting plane and the specimen. In UIS2/UIS objective lenses, the parfocal distance is designed at 45mm.
Working distance and parfocal distance Objective lens mounting position

Practical field of view for = video monitor observation

Image device size *

M(ob)M(video camera adapter)

M(ob): Objective lens magnification M(video camera adapter): Projected magnification for video camera
adapter including photo eyepiece
(refer to Figure 1 for projected magnifications) * Refer to Figure 2 for image device size Figure 1 Video camera adapter and projection magnifications Video camera adapter (Projection lens) U-TV1x-1 + video camera mount adapters U-TV0.63xC U-TV0.5xC-3 U-TV0.35xC-2 U-TV0.25xC Projection magnifications 1x 0.63x 0.5x 0.35x 0.25x

Parfocal distance Working Distance (W.D.)

Focal plane

Figure 2 Imaging device size

For parfocal distance of the LCPLFLN-LCD series objective lenses, refer to the appropriate objective lens page.

Camera format 1/3" 1/2" 2/3"

Diagonal 6.0mm 8.0mm 11.0mm

Horizontal 4.8mm 6.4mm 8.8mm

Vertical 3.6mm 4.8mm 6.6mm

The above table is for standard image device sizes. Check your device size for precise calculation.

4. Relationship between the objective lens's focal length and magnifications Indicated magnifications of UIS2/UIS objective lenses are the values when the focal length of the tube lens is 180 mm.

Figure 3 Imaging device size and monitor magnifications Monitor size (diagonal) Camera format 10" 15" 17" 19" 1/3" 42.3x 63.5x 72.0x 80.4x 1/2" 31.8x 47.6x 54.0x 60.3x 2/3" 23.1x 34.6x 39.3x 43.9x

21" 88.9x 66.7x 48.5x

M(ob)=

Focal length of tube lens f

M(ob): Objective lens magnification f: Objective lens's focal length

Example What is total magnifications for video monitor when objective lens is 50x, video camera adapter U-TV0.5xC, 2/3" video camera and 21" monitor are used ?

43

OPTICAL TERMINOLOGY
Total magnification on the video monitor: m(ob)=50, M(video camera adapter) is 0.5 from Figure 1 and monitor magnification is 48.5 from Figure 3. M(monitor observation)=M(ob)M(video camera adapter)monitor magnification =500.548.5=1213 Practical filed of view for video observation(horizontal side): M(ob)=50, M(video camera adapter) is 0.5 from Figure 1 and horizontal side of 2/3" imaging device is 8.8mm from Figure 2 Practical field of view = for video observation 8.8 (mm) = =352m 50 0.5 Image device size Resolving power formula The following formula is generally used for determing resolution. (Reyleigh formula) = 0.61 N.A. : Wavelength or radiation in use (=0.55m is used for visible light) N.A.: Objective lens N.A. Example MPLFLN100(N.A.=0.90), =0.55m = 0.61 0.3355 0.3355 = = = 0.37m N.A. N.A. 0.90

M(ob) M(video camera adapter)

8. Focal depth of Microscope 6. Numerical Aperture (N.A.) The numerical aperture is a key factor to the performance of objective lens (resolving power, focal depth and brightness). The N.A. is determined by the following formula: N.A.= n sin n=Refraction rate of the medium between specimen and objective lenses. (Air: n=1, oil: n=1.515) : Angle which is made by the optical axis and refraction of the light farthest from the center of the lens. The visual field brightness (B) of the microscope is determined by the following formula in relation to the objective lens magnification (M). The larger the N.A. and the lower the objective magnification, brightness will increase in the factor of the second power. N.A.2 B M2
Numerical aperture

The focal depth refers to the depth of the specimen layer which is in sharp focus at the same time, even if the distance between the objective lens and the specimen plane is changed when observing and shooting the specimen plane by microscope. As human eyes are individually different in the ability of their focus adjustment, each person's perception of the focal depth varies. At present, the Berek formula is generally used, because it gives a focal depth value that often coincides with that obtained through experiments. Focal depth formula Visual observation (Berek formula) D.O.F.= 250,000 (m) + N.A. M 2(N.A.) 2

D.O.F.: Depth Of Focus

: Resolving power of eyes 0.0014 (when optical angle is 0.5 degrees) M: Total magnification (objective lens magnification x eyepiece magnification)
Objective

n=1 (air)

Sample surface

350 0.275 D.O.F. = N.A. M + N.A.2 (=0.55m) This indicates that the focal depth becomes smaller as the numerical aperture becomes larger. Example With MPLFLN100(N.A.=0.90), WHN10: 350 0.275 + = 0.39 + 0.34 = 0.73m 0.90 1,000 0.81

D.O.F. = 7. Resolving Power The resolving power of an objective lens is measured by its ability to differentiate two lines or points in an object. The greater the resolving power, the smaller the minimum distance between two lines or points that can still be distinguished. The larger the N.A., the higher the resolving power.

Video camera In the case of a video camera, the focal depth will vary according to number of pixels of CCD, optical magnification, and numerical aperture. The above-mentioned formula is used as a rough guide only.

44

OPTICAL TERMINOLOGY

9. Aberrations A difference between an ideal image and an actual image that passes through an optical system is called an aberration. 9.1 Requirements for Ideal Image Formation The following three requirements must be satisfied to form an image with no aberration, or an ideal image. (i) All the light rays coming from a single point and passing through an image formation optical system converge on a single point. (ii) Image points, which correspond to object points on the same plane perpendicular to the optical axis, are present on the same plane. (iii) The planar shape of an object and the planar shape of an image that are on the same plane perpendicular to the optical axis have a similarity relation.
Figure 9-1 Requirements for Ideal Image Formation
ii iii

characteristics of glass materials used for the optical system. Expansion of a point image can also be expressed by wavefront aberration that regards the light as waves and takes account of the phase to include the influence of diffraction. (1) Spherical aberration When light rays coming out of an axial object point enter a lens, the light rays with a larger numerical aperture (N.A.) are subjected to stronger refraction power and cross the optical axis in positions with larger differences from the ideal image formation position. The aberration caused this way by different image forming positions due to differences in N.A. of axial light rays is called spherical aberration. (Spherical aberration is proportional to the cube of N.A.)
Figure 9-3 Spherical Aberration

Specimen

Aplanatic tube lens

Object

i Image plane

Objective lens with spherical aberration

Image plane

In an actual optical system, however, it is very difficult to strictly meet the requirements for ideal image formation and this causes aberrations that interfere with image forming performance. 9.2 Classification of Aberrations Aberrations that interfere with image forming performance are classified as shown below in Figure 9-2. Seidels aberration = Expansion of a point image + Curvature of image plane + Deformation
Figure 9-2 Classification of Aberrations
(1) Spherical aberration (2) Coma aberration Seidel's aberration (3) Astigmatism (4) Field curvature Aberration (5) Distortion (6) Longitudinal (axial) chromatic aberration (7) Chromatic aberration of magnification

It is said that objective lenses with larger N.A. have better resolution but worsen spherical aberration. Our advanced design and manufacturing techniques have realized good optical performance even with large numerical aperture. (2) Coma aberration Even though spherical aberration is compensated to be very small, there are cases where light rays coming out of an off-axis object point are not condensed to a single point on the image plane but generate asymmetric blur just like a comet leaving traces. This is called coma aberration.
Figure 9-4 Coma Aberration and Spot Shape on the Image Plane

Specimen

Aplanatic tube lens

Objective lens with coma aberration

Image plane

Chromatic aberration

Types (1) to (3) correspond to expansion of a point image that goes against requirement (i) for ideal image formation in Figure 91. Type (4) corresponds to curvature of image plane that goes against requirement (ii) in Figure 9-1. Type (5) corresponds to deformation that goes against requirement (iii) in Figure 9-1. Types (6) and (7) correspond to color blur of images caused by
45

(3) Astigmatism Even though a lens is compensated for spherical aberration and coma aberration, there are cases where an image of an off-axis object point is not focused to a single point but separated to a concentric line image and a radial line image. This is called astigmatism. When astigmatism is present, a point image blurs vertically and horizontally, before and after the focus position.

OPTICAL TERMINOLOGY

Figure 9-5

Astigmatism and Change in Spot Shape in Different Focus Positions

aberration of magnitude. Many special glass materials are used, e.g., for apochromats (MPlanApo in Olympus), to eliminate chromatic aberration in a wide range from violet light (g-rays with wavelength of 435 nm) to red light (c-rays with wavelength of 656 nm). 9.3 Wavefront Aberration Since a long time ago, aberrations have been used in geometric optics, which considers light as light rays. Microscope optical systems are often used for observation of very small specimens at a wavelength level, and sometimes adopt wave optics, which regards light as waves and handles the phase information, taking account of the influence of diffraction. In such a case, wavefront aberration is used for evaluation. As shown below, when requirements for ideal imaging are satisfied in a microscope optical system, the spherical wavefront (spherical waves) coming from a single point on an object (specimen) is converted to plane waves through an ideal objective lens. The plane waves are converted to spherical waves through an ideal tube lens, and condensed to a single point. The wavefront of these waves is called the ideal wavefront.
Figure 9-7 Ideal Microscope Optical System

(a) (b) (c)

(a)

(b)

(c)

(4) Field curvature An image plane of an object on a plane perpendicular to an optical axis does not always become a plane perpendicular to the optical axis, but it generally becomes a curved plane. This symptom is called field curvature. When field curvature is present, the image is more displaced as it becomes closer to the periphery of the visual field. Therefore, when the center of an image is brought into focus, blur occurs in the peripheral areas of the image. To bring the entire image, including the periphery, into clear focus, it is necessary to adequately compensate for this type of aberration. (5) Distortion When there is no similar relation between a planar shape on an object and a shape on the image plane, this is called distortion. When distortion is present, a square image appears in a shape of a barrel or pin-cushion as shown in Figure 9-6.
Figure 9-6 Distortion
(a) Barrel shape type (a) Pin-cushion type

Specimen Ideal objective lens

Ideal tube lens

Image plane

Spherical wave

Plane wave

Spherical wave

Based on the figure indicated for (1) spherical aberration, the behavior of the wavefront in an optical system that has an aberration is described below.
Figure 9-8 Illustration of Wavefront Aberration
Actual wavefront Specimen

The microscope optical system contains some distortion. When distortion is present, it can bring erroneous results of shape measurements. When a microscope is used for precision measurements, pay close attention to this aberration, for example, by providing it with an aberration compensation function. (6) Chromatic aberration Glasses used for optical systems have different refractive indexes depending on the wavelength. This causes differences in focal length between wavelengths and generates displacement of image forming position. This phenomenon is called chromatic aberration, which is sometimes subdivided into axial displacement on the optical axis, called axial chromatic aberration (or lateral chromatic aberration) and displacement on the image plane, called chromatic
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Ideal wavefront

Objective lens with spherical aberration

A difference (a degree of disagreement) between the ideal wavefront and the actual wavefront shown above is called wavefront aberration.

OPTICAL TERMINOLOGY
9.4 Strehl ratio When a point light source is observed with an aberration-free optical system and an aberrated optical system , the former concentrates the focal point to a point at the image formation position. In contrast, the latter fails to produce a focal point, instead causing a spread in the intensity distribution of the point image (this is known as point spread). The specific appearance of such a point image (i.e. point spread) is shown in Fig. 9-9.
Figure 9-9 Appearance of condensed light in the image plane (point spread)

Aberration-free optical system

Aberrated optical system

With the proportion of light concentrated in the image plane (intensity of light concentrated in the Airy disk) by an aberrationfree optical system serving as 100%, the proportion of light concentrated by an aberrated optical system is known as the Strehl ratio. When graphed, the Strehl ratio reveals peaks in intensity as shown in Fig. 9-10. The higher the SR, the closer an optical system is to being aberration-free.
Figure 9-10 Strehl ratio
Intensity

Aberration-free optical system

100% Aberrated optical system SR

Image plane

A Strehl ratio of 80% is typically called the diffraction limit, and lenses with a lower ratio lack the performance required to serve as an objective lens. A ratio of over 95% means that the lens performance in general observations is comparable to that of an aplanatic lens (which is corrected for spherical aberrations and coma). Note) A laser interferometer is used for actual assessment of optical performance, so assessment is done at a single wavelength. Unless otherwise noted, Strehl ratio measurements are at the e-line (544nm).

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