INTERNATIONAL TURKISH HOPE SCHOOL-CHITTAGONG BRANCH

BIOLOGY WORKSHEET CLASS

TOPIC: .
NAME:.

DATE:

1. In an experiment to study the effect of temperature on the action of an enzyme for coagulating milk. Milk and the
enzyme were first kept in separate test tubes in a water bath at T oC for 15 minutes before they were mixed. The
resulting mixture was kept in the same water bath. The time taken for milk to coagulate was noted and the
experiment was repeated by using water baths at different temperatures. The time taken for the milk to coagulate at
different temperature is shown in the table below.

(i)

(ii)
(iii)

Temperature of water bath

(ToC)

Time taken for coagulation

(minutes)

10

No coagulation occurred.

20

30

40

50

60

No coagulation occurred.

What is the purpose of keeping the milk and enzyme in the water bath for 15 minutes
before mixing?
[01]
_______________________________________________________________________________
At which temperature was the enzyme most active?

[01]

_______________________________________________________________________________
State and explain whether there would be any change in the results if the mixture originally kept at 10 oC was
warmed to 30oC.
[03]
_______________________________________________________________________________
_______________________________________________________________________________

(iv)

_______________________________________________________________________________
What control experiment should be carried out to confirm that the coagulation of milk
was due to the action of an enzyme? What was the expected result?
[03]
_______________________________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________

(v)

State one factor, other than temperature, that can affect the activity of enzymes.
_______________________________________________________________________________

[01]

INTERNATIONAL TURKISH HOPE SCHOOL-CHITTAGONG BRANCH

BIOLOGY WORKSHEET CLASS
TOPIC: .
NAME:.

DATE:

2. The drawing below shows how a certain biochemical reaction takes place inside the small intestine of a mammal:

(i)

Which molecule is an enzyme?

(1 mark)

(ii)

_______________________________________________________________________________
Which are the substrate(s) and product(s) of this reaction?
(4 marks)
_______________________________________________________________________________

(ii)

_______________________________________________________________________________
What is the name of the part of the enzyme that determines the specificity of the reaction?
(1 mark)

(iii)

_______________________________________________________________________________
Draw a simple sketch to show the relationship between enzyme activity and
(1) temperature.
(2) pH.
(1)

(iv)

(2)

(4 marks)
Give an example of this type of reaction in the small intestine and state the products of the reaction.
(2 marks)
_________________________________________________________________________________

________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________
________________________________________________________________________________

INTERNATIONAL TURKISH HOPE SCHOOL-CHITTAGONG BRANCH

BIOLOGY WORKSHEET CLASS
TOPIC: .
NAME:.

DATE:

3. Tom prepared an experiment to investigate the action of an enzyme. The test tubes were placed in a water bath at
37C.
At the beginning
3 minutes later
30 minutes later
2 cm3 of
enzyme
suspension
was added
and stirred

10 cm3 of
egg white

cloudy
(i)

cloudy

Name the enzyme.

clear
(1 mark)

_______________________________________________________________________________
(ii)

Explain why the suspension became clear.

(1 mark)

(iii)

_______________________________________________________________________________
Suggest with reason one method to speed up the action.
(3 marks)
_______________________________________________________________________________
_______________________________________________________________________________

(iv)

_______________________________________________________________________________
Predict and explain the result if the test tube is placed in a water bath at
(1) 80C.

(2 marks)

_______________________________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________
(2) 0C.
(2 marks)
_______________________________________________________________________________
_______________________________________________________________________________
_______________________________________________________________________________

INTERNATIONAL TURKISH HOPE SCHOOL-CHITTAGONG BRANCH

BIOLOGY WORKSHEET CLASS
TOPIC: .
NAME:.

DATE:

4. The following experiment was carried out to study the properties of a washing powder containing a biologically
active substance X.

washin
g
powder
solution

B
film with a
layer of
protein coat

After 2 hours

protein
coat
absent
(i)

Describe the action of substance X on the protein coat.

boiled
washin
g
powder
solution

protein
coat
present
(2 marks)

_______________________________________________________________________________

(ii)

_______________________________________________________________________________
What is the effect of boiling on substance X?
(1 mark)
_______________________________________________________________________________

(iii)

Name substance X. Explain why this washing powder can remove egg stain from clothes. (3 marks)
_______________________________________________________________________________
_______________________________________________________________________________

(iv)

_______________________________________________________________________________
It is not advisable to use this washing powder with water at temperatures above 60oC.
Explain why.
(1 mark)

(v)

_______________________________________________________________________________
It is also suggested not to use this washing powder together with bleaching solution.
Explain briefly.
(2 marks)
_______________________________________________________________________________
_______________________________________________________________________________