Proceedings of the 26th Academic Council held on 18.5.

2012

MBY506ANALYTICALTECHNIQUES VersionNo. Course Pre-requisites/ Co-requisites/ anti-requisites Objectives: ExpectedOutcome: : 1 : Nil

LTPC 3045

ELECTROCHEMICALTECHNIQUES 5hours UnitNo.1 pH Definition and significance, Buffers and their preparation, Derivation of Henderson Hasselbach equation, calculation of pKa with numericals Determination of pH using the pH meter: Concept of a glass electrode, reference electrode, SHE UnitNo.2 CHROMATOGRAPHY 10hours General Principles, Classification of chromatographic methods, Nature of partition forces, performance parameters, retention times & volumes, resolution, peak broadening, and elution., Principles of adsorption, gel permeation, ion exchange and affinity chromatography Principle, procedures and applications of Paper and Thin Layer chromatography Principle, Instrumentation, procedure and applications of Gas Chromatography and High Performance Liquid Chromatography UnitNo.3 ELECTROPHORESIS 10hours Principle and components of electrophoresis unit, Factors affecting electrophoretic mobility, support medium and buffers used Principle, procedure and applications of paper, cellulose acetate, Agarose, and Polyacrylamide electrophoresis, electro-blotting, 2D PAGE, isoelectric focusing and immuno electrophoresis, capillary electrophoresis, PFGE UnitNo.4 CENTRIFUGATIONANDMICROSCOPY 12hours Principle, centripetal and centrifugal forces, factors affecting sedimentation rate, sedimentation co efficient and constant, gradient media, types of centrifuges, preparative and analytical centrifugation. Principles of Microscopy, resolving power, magnification, Compound Microscope Principle and Instrumentation, Types of light microscopes, Phase contrast, Fluorescence, confocal microscope, Electron Microscope - overview of SEM and TEM UnitNo.5 OPTICALSPECTROSCOPY 8hours Electromagnetic spectrum and interaction of electromagnetic radiation with matter Absorption spectroscopy - terms employed, Beer Lamberts Law and its limitations, Quantitative aspects of absorption measurements, Photometric and spectrophotometric designs, Instrumentation of Single, double and split beam instruments Molecular UV-VIS absorption spectroscopy, absorbing species and types of transitions, organic chromophores, Biological applications

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Proceedings of the 26th Academic Council held on 18.5.2012

Textbooks: 1. Willard, Merrit, Dean, Seattle, Instrumental Methods of analysis, CBS Publishers, 2007. ReferenceBooks: 1. Plummer, D.T (1988) An introduction to practical biochemistry. Tata McGraw-Hill Publishing Company 2. Wilson, K and Walker, J (2004) Practical Biochemistry. V Edition, Cambridge University Press. 3. Cooper, G,M (2000) The Cell: Molecular Approach II Edition, ASM Press, Washington, DC 4. Webster, J.G (2005) Bioinstrumentation, John Wiley & Sons. ModeofEvaluation Recommendedbythe BoardofStudieson DateofApprovalbythe AcademicCouncil Continuous Assessment (CATs, Assignments, etc.) and TEE. 30th April 2012 18th May 2012

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