Case Study of Histotechnolgy On

Various Methods of Tissue Embedding

Submitted by
Name Ajay Kumar Reg. No. 11112589 Roll No. - RZ1151A41 Class BSc. MLT (4th Sem)

Submitted to
Mr. Harpreet Singh

Date of Submission
17th April 2013

Qno.1 A hemoglobin and hematocrit were ordered on a patient for a surgical floor. The test was performed on the Coulter LH 750, and the hemoglobin and hematocrit were compatible with previous results. However, the instrument routinely reports the complete CBC, and while observing the entire nine parameters, the operator noticed that the platelet count was only 23,000, a critical value. The delta check on the patient from the previous day showed that the platelet count was 257,000, a significant difference. Corrective action needed to be taken. The operator decided to check the tube that she has just cycled through the instrument for clots, and a small clot was found. How many times should a purple top tube be inverted once drawn to prevent clotting?

Case Study of Haematology On

Cytochemical Stains and Cytogenetic Analysis

Submitted by Name Ajay Kumar Reg. No. 11112589 Roll No. - RZ1151A41 Class BSc. MLT (4th Sem)

Submitted to Mr. Vinod Kumar Date of Submission 17th April 2013

References:
Websites: http://www.sigmaaldrich.com/life-science/cell- biology/antibodies/antibodiesapplication/protocols/fish.html

http://www.scribd.com/doc/50190592/Cytochemistry

Books: Practical Hematology 10th edition, by Dacie and Lewis Page No. 318- 328

Clinical hematology: theory and procedures, 4th edition, By Mary Louise Turgeon, Page No. 466-470

Hematology: clinical principles and applications, 3rd edition, by Bernadette F. Rodak, George A. Fritsma, Kathryn Doig, Ph.D. Page No. 399-402

Blood: principles and practice of hematology, 2nd edition, by Robert I. Handin, Samuel E. Lux, Thomas P. Stossel Page No. 17-19

Wintrobe's clinical hematology. 12th edition, by John P. Greer, M.d. Page No. 14

References:
Websites: http://www.scribd.com/doc/79052419/21/Histochemical-fixatives Books: Medical Lab Science: Theory & Practice, by J Ochei & A Kolhatkar Page No. 413- 420

Histophatologic Techniques, 2nd edition, by Bruce-Gregorios, Jocelyn Page No. 76- 91

INDEX
S No. 1 2 3 4 5 6 7 8 9 10 11 12

Topic
Introduction Paraffin Wax Embedding Substitute of Paraffin Wax Celloidin Embedding Nitrocellulose Embedding Gelatin Embedding Resin Embedding Agar Embedding

Page No.

INDEX
S No
1

Topic Introduction Myeloperoxidase Stain Sudan Black B Periodic Acid Schiff Stain Neutrophil Alkaline Phosphate Esterase Stain Fluorescence In Situ Hybridization

Page No.

2 3 4 5 6 7 8 9