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Forward Genetics 2:
Complementation, Epistatic Relationships,
Sequencing Analysis, & Prediction of Protein
Function in Arabidopsis thaliana with emphasis on
trichome morphogenesis
(*companion paper to Forward Genetics: An approach to the mapping and identification of a mutation in
trichome morphogenesis of Arabidopsis thaliana (4/24/2003) pgs 1-18)
Lab #3
A02-92-6779
Group #6
-1-
Abstract:
Based on previous investigation1 the location of the mutation of interest (mut-1) was
mapped to chromosome 5 of Arabidopsis on the BAC K14B20. A complementation test
was preformed and the outcome was an allelic relationship between mut-1 and mut-3.
This complementation group was the focus of further investigation. The gene of interest
was discovered through the use of Agrobacterium transformation. A wild-type gene
construct was inserted to rescue the mutant phenotype. The construct that produced a
three prong trichome (wt) was the clone AT5G65930.1 which contains the ZWICHEL
gene. ZWICHEL is one of the candidate genes located on K14B20. The actual names of
mut-1 and mut-3 were determined to be zwi-3 and zwi-9311 after sequence comparison
analysis using MacVector. The mut-1 and mut-3 sequences were compared to the
Complete ZWI gene2. A double-mutant test involving an and nok displayed that AN is
epistatic to NOK. Final analysis was performed on the possible role of the functional
domains and structural motifs of the ZWICHEL protein in relation to its role as a kinesin-
like calmodulin-binding protein (KCBP) involved in trichome morphogenesis3.
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Lab #3
A02-92-6779
Group #6
-9-