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800/234-5333 517/372-9200
ISO-GRID
800/234-5333 517/372-9200
1. SET-UP. Attach the short arm of the Y tube without the shut-off valve to the vacuum manifolds base (or vacuum flask, if used). The tubes arm with the shut-off valve is left free until step 7. 5. ADD DILUENT. Add sterile diluent. Most of it will remain on the stainless steel prefilter.
2. PLACE MEMBRANE FILTER. With the vacuum source on, and manifold vacuum and Y tube valves open, place a membrane filter on the base of the filtration unit.
3. CLOSE FILTRATION UNIT. Swivel the hinged filtration unit to the closed position.
6. ADD SAMPLE. Pipette a known quantity of sample into the filtration unit, followed by additional diluent to ensure thorough mixing.
800/234-5333 517/372-9200
Sample Results
8. FILTER. Close the valve on the free arm of the Y tube to draw the sample and diluent through the membrane filter.
9. TRANSFER. Without turning off the valve, open the filtration unit. If no droplets remain on the membrane filter, aseptically transfer the filter to the desired agar plate.
800/234-5333 517/372-9200
ISO-GRID Products
Equipment
6810 6800 6801 6802 6803 6804 6805 6806 6807 6808 6809 9463 9464 9735 1-Place Filtration Manifold 3-Place Filtration Manifold 6-Place Filtration Manifold Membrane Filters (100) Filtration Unit Filtration Unit Clamp Methods Manual Filtration Unit Base Filtration Unit Funnel ISO-GRID Starter Kit - 200 membrane filters, 4 filter units, 2 filter unit clamps, Methods Manual Paddle Bags (500) Pipettor (100 L-1 mL) Pipette Tips Incubator w/thermometer
6803
6800 9463
6802
Enzyme Products
6850E 6851E 6852E 6853E 6854E Papain APUG (alkaline protease) AMG (amyloglucosidase from rhizopus mold) Cellulase Hemicellulase
9464
6804
Culture Media
6900E 6901E 6902E 6903E 6904E 6905E 6906E 6907E 6909E 6910E 6912E Bacteriological Agar EF-18 Agar - for Salmonella detection LMG Agar - for E. coli / coliform enumeration BMA Agar - for confirmation of E. coli YM-11 Agar - for enumeration of yeasts and molds TSAF Agar - for total bacterial count LM-137 Agar (125 g + supplement pool) - for Listeria detection SD-39 Agar - for enumeration of E. coli / E. coli O157:H7 Tetrathionate Broth Base - Salmonella Enrichment Broth SCCRAM Broth - Salmonella Enrichment Broth Chlortetracycline-HCL - antibiotic supplement for YM-11 Agar
9735
Incubator w/thermometer
620 Lesher Place Lansing, MI 48912 800/234-5333 (USA and Canada only) 517/372-9200 fax: 517/372-2006 e-mail: neogen-info@neogen.com www.neogen.com
Neogen Corporation, 2001. Neogen is a registered trademark and ISO-GRID is a trademark of Neogen Corporation, Lansing, MI. p-ISO-GRID4-1001