FISH Probes - Fluorescent In Situ Hybridization Probes

12/11/2012 13:38

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FISH Probes - Fluorescence In Situ Hybridization

Description
FISH (fluorescence in situ hybridization) is a cytogenetic technique used to detect or confirm abnormalities in any of the 24 chromosomes. The sample DNA (metaphase chromosomes or interphase nuclei) is first denatured, a fluorescently labeled probe of interest is then added to the denatured sample mixture, which binds to only those parts of the chromosome with which they show a high degree of sequence similarity, and hybridizes with the sample DNA at the target site as it re-anneals back into a double helix. One can then use a fluorescent microscope to identify if a signal is present or not. Unlike most other techniques used to study chromosomes, FISH does not have to be performed on cells that are actively dividing. This makes it a very versatile procedure.

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How does FISH work?

FISH is useful, for example, to help a researcher identify where a particular gene falls within an individual's chromosomes. Here's how it works: Make a probe complementary to the known sequence. When making the probe, label it with a fluorescent marker, e.g. fluorescein, by incorporating nucleotides that have the marker attached to them. Put the chromosomes on a microscope slide and denature them. Denature the probe and add it to the microscope slide, allowing the probe hybridize to its complementary site. Wash off the excess probe and observe the chromosomes under a fluorescent microscope. The probe will show as one or more fluorescent signals in the microscope, depending on how many sites it can hybridize to.

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Our Services and Products

Empire Genomics is the leader in the development and manufacturing of custom labeled FISH probes, providing uniquely tailored FISH probe solutions to meet the individual needs of researchers and clinicians alike. Our FISH technology allows for fast, accurate detection and confirmation of chromosomal aberrations across the genome.

This probe is for RESEARCH USE ONLY The probe has NOT BEEN TESTED ON CLINICAL SAMPLES and is NOT VALIDATED.
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FISH Probes - Fluorescent In Situ Hybridization Probes

12/11/2012 13:38

Copyright 2012 Empire Genomics. All Rights Reserved.

http://www.empiregenomics.com/Fluorescent-In-Situ-Hybridization/FISH-Probes/FISH-Probes.html

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