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Code 9A23304 II B.Tech I semester (R09) Regular Examinations, November 2010 MICROBIOLOGY
(Biotechnology)

1
Max Marks: 70 Answer any FIVE questions All questions carry equal marks

Time: 3 hours

1. (a) Write a detailed account of major events in the eld of microbiology. (b) Write a typical characteristics features of bacteria and algae. 2. (a) Write characteristics of bacteria with special reference to Archaea and Eubacteria. (b) Describe briey about dierent classication systems in microbiology. 3. (a) Describe macro and micronutrients required by microorganisms for their growth. (b) Describe dierent pure culture methods spread plate , pour plate and streak plate methods. 4. (a) Write about dierent methods for preservation of microorganisms. (b) Write about bacterial staining techniques.

5. (a) Write about denition of infection, diseases and pathogens with examples. (b) Write about virulence and virulence factors of inuenza and tuberculosis. 6. (a) Write 3 examples of animal viruses and plant viruses. (b) Describe the classication of viruses.

7. (a) Describe the bacterial replication and also mechanism of lysogeny. (b) Write about DNA viruses and RNA viruses.

8. (a) Write a detailed account on dierent method of identication and invitro cultivation of viruses. (b) Write about assay of animal viruses.

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D L

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Code 9A23304 II B.Tech I semester (R09) Regular Examinations, November 2010 MICROBIOLOGY
(Biotechnology)

2
Max Marks: 70 Answer any FIVE questions All questions carry equal marks

Time: 3 hours

1. (a) Explain the theory of spontaneous generation. (b) Write an account of microbial diversity. 2. (a) Write two scientists name and their contribution in the eld of microganisms. (b) Write about major characteristics used in taxonomy of microganisms. 3. (a) Write about microganisms classication based on nutrition. (b) What are the special techniques used for cultivation of anaerobes. 4. (a) Write about working and primary stock cultures-Lyophilisation.

(b) Write about inuence of environmental factors on the growth microganisms. 5. (a) Write about denition of infection diseases and pathogens with examples. (b) Write about virulance and virulance factors of inuenza and tuberculosis. 6. (a) Write about the properties of viruses and illustrate the virus structure. (b) Write about the application of viruses in biotechnology industry. 7. (a) Describe the bacterial virus replication and also mechanism of lysogeny. (b) Write about DNA viruses and RNA viruses

8. (a) Write a detailed account on dierent methods of identication and invitro cultivation of viruses. (b) Write about assay of animal viruses.

T N

W U

R O

D L

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www.jntuworld.com

Code 9A23304 II B.Tech I semester (R09) Regular Examinations, November 2010 MICROBIOLOGY
(Biotechnology)

3
Max Marks: 70 Answer any FIVE questions All questions carry equal marks

Time: 3 hours

1. (a) Write about germ theory of diseases (b) Write dierent techniques to identify micro organsims. 2. (a) What is the signicance of microbial classication? Describe the classication of CarlWooese et al. (b) Describe dierent taxonomic ranks in microbiology with special reference to bacteria. 3. (a) Write briey on nutritional classes of microorganisms.

(b) Write a detailed account on dierent types of media- selective media, dierential media and enrichment media. 4. (a) Write about use of agar glants, agar stabs for preservation of microorganisms. (b) Write about aims and principles of biochemical test - sugar fermentations IMVIC tests and catalase production. 5. (a) Write about denition of infection, diseases and pathogens with examples. (b) Write about virulence and virulence factors of inuenza and tuberculosis. 6. (a) Write 3 name of animal viruses and plant viruses. (b) Describe the classication of viruses.

7. (a) Describe the bacterial replication and also mechanism of lysogeny. (b) Write about DNA viruses and RNA viruses. 8. (a) Write a detailed account on dierent methods of identication and invitro cultivation of viruses. (b) Write about assay of animal viruses.

T N

W U

R O

D L

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www.jntuworld.com

Code 9A23304 II B.Tech I semester (R09) Regular Examinations, November 2010 MICROBIOLOGY
(Biotechnology)

4
Max Marks: 70 Answer any FIVE questions All questions carry equal marks

Time: 3 hours

1. (a) Write two scientists names and their contribution in the eld of microbiology. (b) Write typical characteristics features of fungi an their signicance role in medicine. 2. (a) What are the dierent growth factors and their functions in microbial metabolism? (b) Describe cultivation of microorganisms and also describe the role of solidifying agents in media. 3. (a) Write about preservation of microorganisms-use of sterile oil and cryopreservation . (b) Write a detailed account on simple staining dierential staining and endospore staining. 4. (a) Write about three domains of life.

(b) Write briey about biochemical, immunological and genetical characters referring to Taxonomy of microorganisms. 5. (a) Write about denition of infection, diseases and pathogens with examples. (b) Write about virulance and virulance factors of inuenza and tuberculosis. 6. (a) Write about the properties of viruses and illustrate the virus structure. (b) Write about the application of viruses in biotechnology industry. 7. (a) Describe the bacterial replication and also mechanism of lysogeny. (b) Write about DNA viruses and RNA viruses. 8. (a) Write a detailed account on dierent methods of identication of viruses and in vitro cultivation of viruses. (b) Write about assay of animal viruses.

T N

W U

R O

D L

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