Professional Documents
Culture Documents
http://www.pub.ac.za
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Cell Nucleus
Chromosome
DNA
DNA Packaging:
www.csu.edu.au/.../molbol/images/8_10.jpg
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http://www.pub.ac.za
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http://www.ncbe.reading.ac.uk/DNA50/Resources/wc1993.gif
DNA Structure
DNA
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Nitrogenous Bases
http://www.ca.uky.edu/Agripedia/glossary/agriart/
Nitrogenous Bases
Purines
Pyrimidines
http://www.ca.uky.edu/Agripedia/glossary/agriart/
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Nucleotides
http://ghs.gresham.k12.or.us/science/ps/sci/ibbio/chem/notes/chpt14/nucleotide1.gif
http://bvc.kribb.re.kr/download/image/nucleotide2.gif
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http://www.emc.maricopa.edu/faculty/farabee/biobk/BP2.gif
RNA
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Types of RNA
messenger RNA (mRNA): Carries the genetic information out of the nucleus into cytoplasm for protein synthesis ribosomal RNA (rRNA): Makes up ribosome, constitutes 50% of a ribosome, which is a molecular assembly involved in protein synthesis transfer RNA (tRNA): Decodes the information in mRNA
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http://scienceblogs.com/clock/upload/2006/11/a2%20DNA-RNA-ribosome2.jpg
Proteins
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Cellular Proteins
Enzymes Catalyze chemical reactions Cytoskeletal proteins Give cell its shape and motility Receptor proteins Bind to molecular stimuli from outside the cell Signal transduction Relaying of signal into intracellular events Perform almost every other function in a cell
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"My mind was, that a dogma was an idea for which there was no reasonable evidence. You see?!" And Crick gave a roar of delight. "I just didn't know what dogma meant. And I could just as well have called it the 'Central Hypothesis,' or you know. Which is what I meant to say. Dogma was just a catch phrase. - Horace Freeland Judson, The Eighth Day of Creation
http://www.pub.ac.za
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http://www.pub.ac.za
http://www.pub.ac.za
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http://www. pub.ac.za
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Messenger RNA
mRNA Copies information from DNA to bring out into cytoplasm Is used by transfer RNA (tRNA) and ribosomes to produce protein Is made up of codons as informational units
Codon
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Genetic Code
the letters of the genetic alphabet... are the nucleotides A, T, G, & C of DNA unit of information is CODON = genetic 'word' 3 nucleotides = 1 codon (word) = 1 amino acid Amino acids are the building blocks of proteins Amino acid : word :: protein : sentence
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Stop codons mark the end of the protein Amino acid : word Protein : sentence
www.accessexcellence.org
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Recombinant DNA
DNA molecules formed when segments of DNA from two different sources often different species are combined in vitro (in a test tube) Central to genetic engineering
Direct manipulation of genes for practical purposes
DNA/gene cloning
The production of multiple copies of a single gene
GENE CLONING!!!
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Ligase
Joins DNA strands together
Plasmid DNA
Circular bacterial DNA (replicate separately from bacterial DNA)
Gene/DNA of interest
Foreign gene that is introduced into the plasmid DNA and evetually the bacterium
http://fig.cox.miami.edu/~cmallery/150/gene/sf15x1box.jpg
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Plasmid DNA
Double stranded, circular Separate from bacterial DNA Can replicate independently Carries accessory genes antibiotic resistance, toxins, etc.
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Visualizing DNA
Visualizing DNA
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Visualizing DNA
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
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http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
Sanger Method
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Automated Sequencing
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/sequencing.html
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www.revolt.it
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PCR
library.thinkquest.org
PCR
library.thinkquest.org
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PCR
library.thinkquest.org
PCR
library.thinkquest.org
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