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Boil half of the seedlings for 5minutes. Decant, allow to cool and sterilize with 100ml formalin for 5 minutes. Drain.
KOH vials were suspended inside, thermometers were inserted and observed.
Fill thermoflask with the other half of the seedlings and suspend a small vial of KOH inside. Cover with cork, a thermometer sticking out of it. Make sure thermometer is buried in the seedlings.
Explanation The temperature of the boiled seedlings was higher compared to the unboiled seedlings on the first 15 minutes primarily it is due to the heat absorbed by the seeds when boiled. After a period, the temperature of the boiled seeds lowered while the temperature of the unboiled seeds continuously increases. The main purpose of sterilizing the boiled seedlings with formalin is to kill or prevent other factors such as bacteria or such that will affect the setup. Formalin is used to kill bacteria, which are present in decomposition of materials. VI. Conclusion We therefore conclude that the seedlings respire aerobically because of the presence of oxygen that is trapped inside the flasks. Furthermore, we also concluded that germinating seedlings release heat, while nongerminating seedlings do not. Lastly, we observed that respiration could not take place in boiled seeds therefore there is no heat produced.
Record initial temperature and at 15minute interval for two hours in Table 1 of the question sheet.
V. Results
Bolied Seedlings 26 C 30 C 28 C 25 C 24 C 23 C
Unboiled Seedlings 26 C 26 C 27 C 29 C 30 C 31 C