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PHYTOCHEMICAL AND IN-VITRO STUDY OF ETHYL ACETATE EXTRACT OF Spondias pinnata

A thesis report, submitted to the Department of Pharmacy, East West University, in partial fulfillment of the requirements of the Degree of Pharmacy

Submitted by Md. Nasidul Islam ID 2008-3-70-098

Research Supervisor Kh. Tanvir Ahmed Senior Lecturer Department of Pharmacy East West University Aftabnagor, Rampura, Dhaka.

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This Research Paper is dedicated to Almighty ALLAH The most Gracious The most Merciful & To My Parents

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Declaration by the Research Candidate

I, Md. Nasidul Islam, hereby declare that the dissertation entitled Phytochemical and in-vitro

study of methanolic extract of Spondias pinnata, submitted by me to the Department of

Pharmacy, East West University, Bangladesh, in the partial fulfillment of the requirement for the award of the degree of Bachelor of Pharmacy is a bonafide record of original research work carried out by me in 2012 under the supervision and guidance of Kh. Tanvir Ahmed, Senior lecturer, Department of Pharmacy, East West University and it has not formed the basis for the award of any other Degree/Diploma/Fellowship or other similar title to any candidate of any University.

Place Dhaka

Signature of the Candidate Md. Nasidul Islam

_______________________ Date: 23/ 12 / 2012 ID# 2008-3-70-098

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Certificate

This is to certify that the thesis submitted to the Department of Pharmacy, East West University, Bangladesh, in partial fulfillment of the requirement for the Degree of Bachelor in Pharmacy, was carried out by Md. Nasidul Islam ID. 2008-3-70-098.

Sufia Islam, Ph.D Chairperson and Associate Professor Department of Pharmacy East West University Aftabnagor, Rampura, Dhaka

Certificate

This is to certify that the thesis Phytochemical and in-vitro study of methanolic extract of

Spondias pinnata submitted to the Department of Pharmacy, East West University, Bangladesh, in
partial fulfillment of the requirement for the degree of Bachelor of Pharmacy was carried out by Md. Nasidul Islam ID. 2008-3-70-098, under my guidance and supervision and that no part of the thesis has been submitted for any other degree. I further certify that all the sources of information, laboratory facilities availed of this connection is dully acknowledged.

Kh. Tanvir Ahmed

Senior lecturer Department of Pharmacy East West University Aftabnagor, Rampura, Dhaka

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Acknowledgement

First, all praise and glory are to Allah for all the bounties granted to me and only with His guidance and help this achievement has become possible. I wish to express my thanks and gratitude towards my supervisor Kh. Tanvir Ahmed, Senior Lecturer, Department of Pharmacy, East West University, for his constant encouragement, helpful discussions and able guidance throughout this study. His generosity with time and constructive comments were of vital support and were above the call of duty. I would also like to specially thank Md. Amran Howlader, Senior Lecturer, Department of Pharmacy; Apurba Sarkar Apu, Senior Lecturer, Department of Pharmacy, East West University for the continuous support. Their advice and instructions helped me to get the best results. I am indebted to Dr. Sufia Islam, Chairperson and Associate Professor, East West University, for her kind support and the permission to use the facilities available at the Department of Pharmacy, East West University, as well as for her encouragement to carry out research. I would like to convey deepest love and obedience to my caring parents and elder sister for their support and guiding me all through my life until today, which keeps me strong and honest to do the things I needed to do. Finally I would also like to thank all the students especially Mohiuddin, Md. Shamimuzzaman, Md. Mahfuz-e alam, A. K. M. Radowan Ahmad, Md. Mustakim Billah, Md. Aminul Islam, Keya, Farzana Haq, Naifa Akhter, Tasnuba Karim, Laboni, Sumaya, Tamanna for their technical direction and co-operation which enabled me to work in a very congenial and comfortable atmosphere.

vii Abstract Spondias pinnata is an evergreen parasitic plant under the family anacardiaceae and used traditionally in medicinal system. This plant reported to have in vitro antioxidant activity (non-enzymatic hemoglobin glycosylation), and antibacterial activity. The intention of the study was to emphasize on the separating the compounds of methanol extracts of S.pinnata according to their polarity and evaluate their cytotoxicity, antioxidant activity and antimicrobial activity. To fulfill this purpose I did partition of the extracts of S.pinnata into Ethyl acetate solvent system. Then evaluate the antioxidant activity , cytotoxic property by using brine shrimp lethality bioassay. After collection, identification and drying the tree sample, extraction was performed by using methanol solvent system. Then partition was done. Then extracted components were used to evaluation.

Key Words: Spondias pinnata, Brine shrimp lethality assay, Ethyl acetate

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List of Contents

CHAPTER 1: CHAPTER 2: CHAPTER 3: CHAPTER 4: CHAPTER 5: CHAPTER 6: CHAPTER 7:

INTRODUCTION.1-17 LITERATURE REVIEW ....18-23 PLANT DESCRIPTION.....24-34 COLLENTION, DRYING AND EXTRACTION...35-38 DESIGN OF IN-VITRO TEST.......................................................39-41 METHODS AND MATERIALS.42-66 RESULTS AND DISCUSSIONS...67-79

Conclusion.......80 References.....81-83