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Medium
Appearance of negative result White colonies / red medium Colorless and translucent colonies No greenish metallic sheen Orange medium (no RED ring on the surface) Yellow medium Yellow brown medium No change in color of medium (green) No growth and no change in color
Appearance of Positive result Yellow medium, yellow colonies Red colonies, red medium Greenish metallic sheen w/ blue black center in transmitted light Presence of Pink to Red ring on the surface of the broth Red medium
Manitol Salt Agar -M. luteus -E. coli (+) MacConkey Agar -E. aerogenes (-) Eosin-Methylene Blue agar -E. coli (+)
Neutral red
Indole Production
Tryptone Broth
MR test
Nutrient broth + glucose -E. aerogenes(+) -E. coli(-) Simmons citrate agar -E. aerogenes(+) -E. coli (+) O-F medium (w/ oil) -P. aeruginosa (-) -E. coli (+) O-F medium w/o -P. aeruginosa (+)
Green slant
Green
Green
No color change
yellow
-E. coli (+) Triple Sugar Iron -B. subtilis (-) -M. luteus (-) Starch Agar -B. subtilis (+) -E. aerogenes (facultative anaerobe) -P. aeruginosa (strict aerobe) -B. subtilis (+) Nutrient gelatin -M. luteus (-) Sugar fermentation broth -E. aerogenes (+) Red -B. subtilis(-) -E. coli (+) Notrate broth -S. aureus(-) -M. luteus(+) Nutrient agar -E. aerogenes(+) -E. aerogenes(+) Lysine Iron Agar -K. oxycatoa (-) purple White Pale yellow Pale yellow Clear white Red
Yellow butt, red slant with air production Blue-black color surrounding the bacterial growth
Thioglycollate medium
Pale yellow
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Gelatin hydrolysis Sugar fermentation Nitate reduction test Catalase test Glucose fermentation
Solidified agar
Agar becomes liquid Yellow medium with air trapped inside the inverted tube red Presence of bubbles Purple for lysine decarboxylation
Yellow