Biotechnology Biotechdesk Catalog 2006 07

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CATALOG 2006-07
Biotech
We at Biotech Desk Pvt. Ltd. wish to thank all our customers who have believed in us, the services we render and the products we sell. We endeavor to supply you goods that deliver and try to do so in hassle-free manner. We have grown significantly since our inception in 2003, and managed to deepen our loyal customer base that spans research laboratories and industries throughout India. We pride ourselves in being a knowledge-based company and realize the importance of supplying the right product appropriate for a particular experiment, and in the right manner taking care of issues like temperature-sensitive transportation. Biotech Desk Pvt. Ltd. supplies research tools and reagents for: Molecular l Biology l Cell Biology l Proteomics l Immunology
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Be it DNA isolation, In vitro transcription, Genomic cloning, RNAi, Realtime PCR, or Signal transduction, we have a product to suit every customers need. Biotech Desk also offers research services: Gene synthesis l Oligo synthesis l Peptide synthesis l Custom antibody production l cDNA library construction l Site directed Mutagenesis l BAC Library construction l Cloning and related services.
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Biotech Desk Pvt. Ltd. also offers consultancy services to startups in the field of Biotechnology. Biotech Desk Pvt. Ltd. 4F5 Ballad Estate Tarnaka Hyderabad 500017 India Tel: 040-27017477/ 65916167 Fax: 040-27016168 support@biotechdesk.com www.biotechdesk.com
Biotech
CONTENTS
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DNA/ RNA PURIFICATION

Nucleic Acid Isolation
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X-RAY CRYSTALLOGRAPHY ........................................ 77

Crystal Screens, Cystal Optimization, Heavy Atom Derivatization, Consumables, Cryo Crystallization
PCR ............................................................................................ 5
Polymerase Chain Reaction, PCR Optimization, Polymerases, Real Time PCR, DNTPs / Ladders / PCR Enhancer / Buffers, Custom Primer / Oligo Synthesis & Modifications
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ENZYME DETECTION REAGENTS AND ASSAY KITS ......................................................................... 83

MMPs, Viral Proteases, Proteases, Glycosidases, Phosphatases, Phospholipases, Galactosidase, -secretase, Peroxidases-redox Enzymes, Cytochrome P450 & Oxidases, Amyloids, Apoptosis
RT-PCR .................................................................................. 14
Reverse Transcriptases, Kits for RT-PCR/Realtime RT-PCR
CLONING .............................................................................. 16
PCR Cloning, CopyControl Cloning Systems, BAC Cloning, Cosmids & Phage Extracts, Screening and Electroporation, Competent Cells
SIGNAL TRANSDUCTION ............................................... 97

GTP-Ras, GTP-Ran, GTP Heterotrimer, GAP, GTPase, GPCRs, Importins, P13K, PTK, PKA, PKB, PKC, CamK, Cyclin dep. Kinase, Substrates, Inhibitors, Phosphatases,
cDNA LIBRARIES AND CLONES ................................... 25

Ready Clones, cDNA/BAC Clones,
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ANTIBODIES ..................................................................... 113

*Primary, Secondary Substrates, Biotin-Avidin
IN VITRO TRANSCRIPTION .......................................... 29

Prokaryotic Polymerases, Probe synthesis, RNA Amplification, Transcripts for Translation,
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RECOMBINANT PROTEINS
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116
MICROARRAY ..................................................................... 33
Selection guide and Related Items, Amplification Kits
Prion Proteins, Viral Proteins, Transcription Factors, Hematopoetins, Nuclear Receptors, Cytokines, Growth Factors
RNAi ....................................................................................... 36
shRNA Libraries, Vectors, Transfection kits, Making shRNA
PEPTIDE SYNTHESIS ................................................... 127

Amino acids, Resins and reagents, Building Blocks, Catalogue Peptides
MODIFYING ENZYMES ................................................... 38

Nucleases, Glycosylases, Phosphatases, Kinases/Ligases, DNA - binding proteins, Restriction Enzymes
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CUSTOM SERVICES ....................................................... 131

Genomic & Proteomic Services, Antibody and Other Services
TRANSPOSOMICS ............................................................. 50
Insert Promoters, Origin of replication, Plasmid Rescue, Insert Signal Peptide, Custom Transposons, In-vivo mutagenesis, Protein Engg.
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FLUORESCENCE ............................................................. 134

Fluorescent Dyes, Probes, FRET Probes, Quenchers, Protein detection Calcium Detection, Cell Viability, Proliferation & Toxicity,
SEQUENCING ...................................................................... 55
Transposon Insertion kits, Kits for automated & manual Seq
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CYTOGENETICS .............................................................. 149

StarFish Chromosomal Paints,
PROTEIN PURIFICATION ............................................... 57

Bacterial lysis, Affinity Chromatography
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NEW TECHNOLOGIES .................................................. 151

APA Gene Technology, Cloning Vector, Eucaryotic Expression System
INSTRUMENTS ................................................................... 59
Electrophoresis, Electro blotting, Centrifuges, Cryogenics, Homogenizers, Mixers, Stirrers, Shakers, Water Baths, Hybridization Ovens, Electroporation Cuvettes, Transilluminators, Crosslinkers, General Lab Equipments
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INDEX ..................................................................... (i) - (viii)
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DNA PURIFICATION
Kits for DNA Extraction and Purification from EPICENTRE
PlasmidMAX DNA Isolation Kit

Simple, rapid miniprep-type procedure - Scalable to larger volumes High yields of highly purified plasmid DNA of sequencing quality Uses no toxic organic solvents
MasterPure DNA Purification Kit for Blood

Purify genomic DNA up to 10 g from as little as 325 l of blood High DNA purity- A260/280 ratios of 1.8-2.0 - DNA is PCR ready No organic solvents, no columns
MasterPure Plant Leaf DNA Purification Kit

Versatile- recover DNA from leaves of many plant species Rapid- purify DNA in less than 1 hour Recover DNA of higher integrity than column-based purification methods. Takes care of PCR inhibitors like polyphenolics and polysaccharides
SoilMaster/ WaterMaster DNA Extraction Kit

Rapid-extract PCR-ready soil / water high M.W. DNA in less than 45 minutes No need for dedicated equipment or bead beating or organic reagents Removes PCR inhibitors like humic and fulvic acids
MasterPure DNA Purification Kit

Extensive sample rangepurify DNA from any sample, every time High purity- O.D.260/280 ratios consistently between 1.8 and 2.0 High yields, High sensitivity Safe- no phenol, chloroform, or other caustic solvents
MasterPure Yeast DNA Purification Kit

Higher yields of yeast chromosomal DNA than other kits Versatile- recover DNA from a wide variety of yeast species Purify DNA in less than 40 minutes Eliminates bead beating, enzymatic lysis and organic extractions
Biotech Desk Pvt. Ltd.
Tel: +91-40-27017477/ 65916167
Fax: +91-40-27016168
e-mail: support@biotechdesk.com
CUSTOM GENE SYNTHESIS

Why waste time cloning and sequencing your gene when we do it all for you!
Gene synthesis at SPECIAL PRICES Includes insertion into vector of choice
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Includes expression optimization Includes DNA sequencing data plus chromatogram 100% fidelity GUARANTEED Lyophilized plasmid DNA provided E.coli culture transformed and stab provided at minimal cost Expression systems: Bacterial (E.coli), Yeast (Pichia pastoris) Baculovirus (Insect Cell), Mammalian (Mouse)
RANTE UA
100%
We guarantee a quick turnaround-time, unbeatable pricing and large inserts!
OTHER GENOMIC SERVICES BAC LIBRARY CONSTRUCTION

Plasmid library construction Lambda library construction Site-directed mutagenesis Large-construct DNA engineering APA genome walking service Subcloning Gap filling 5' and 3' RACE of first-strand cDNAs 5' and 3' genomic walking Identifying intron/exon junctions Identifying transgene/genomic DNA junctions Identifying gene traps and transposoninsertion location We guarantee coverage We perform HMW DNA extraction at our facilities We have experience with plant, fungal, bacterial and animal libraries We have extensive experience with GC-rich organisms and marine bacteria Our arrayed BAC libraries are shipped in bar-coded microplates Shipment temperature of non-arrayed libraries is always monitored using a BLUE FLAG Temperature Data logger We provide technical support
Biotech
Tel: +91-40-27017477/ 65916167 Fax: +91-40-27016168 e-mail: support@biotechdesk.com
RANTE UA
CUSTOMER SATISFACTION
Millions of Clones
Just identify your clone from:
Nucleotide GenBank Accession # Gene symbols Clone ID (cDNA only) Yeast ORF ID, Record # We can ship them as glycerol stocks or on Isocode paper
Mammalian cDNA Libraries and Clones

Mammalian Gene Collection (MGC) Assay-Ready MGC cDNA BioTrack Human Gene Subsets Human ORF Collection Human Freedom ORFs Incyte Gene Collection (IGC) Mammalian IMAGE ESTs Other ESTs Brain cDNA Libraries Incyte cDNA Libraries
Non Mammalian cDNA Libraries and Clones

Brucella ORF Collection C. jejuni ORF Collection Zebrafish cDNA Library Zebrafish Gene Collection (ZGC) Zebrafish IMAGE cDNAs Zebrafish IMCB, Morpholino and NEIBank cDNA Clones Collections Drosophila Gene Collection (DGC) Xenopus IMAGE cDNAs C. elegans ORF, ORF-RNAi and Promoter Collections
Yeast cDNA Libraries and Clones

Candida albicans Genomic Library HA-Tagged Yeast Strains Yeast Insertional Mutant Strains Yeast Knockout Strains Yeast Magic Marker Strains Yeast ORF Collection Yeast TAP Fusion Collection Yeast Tet-Promoter Hughes Strains
BAC Clones
FISH Mapped BAC Clones GenMap Human BAC Collection Disease-specific Human Mapped BAC Collections Mere Mouse Mapped BAC Collection CalTech Human and Mouse BAC Clones
Biotech DeskPvt. Pvt. Ltd. Biotech Desk Ltd. 4F 5 Ballad Estate, Tarnaka,Hyderabad-500 Hyderabad-500 017,india 4F 5 Ballad Estate, Tarnaka, 017,India. Tel: +91-40-27017477 / 65916167 Fax: Fax: +91-40-27016168 support@biotechdesk.com Tel: +91-40-27017477/ 65916167 +91-40-27016168 Email: e-mail: support@biotechdesk.com
CUSTOM PEPTIDE SYNTHESIS

From 5 mg to 100mg and more For purity levels ranging between 70% to >98% N-terminal and C-terminal modifications Conjugation to carrier Peptides for raising antibodies GMP Grade peptides Labelled peptides with fluorophore Phosphopeptides Glycopeptides Lipopeptides Cyclic Peptides DNA-peptide conjugates Drug-peptide conjugate
Biotech Desk Pvt. Ltd 4F 5 Ballad Estate, Tarnaka, Hyderabad 500 017 India Tel: +91-40-27017477/ 65916167 Fax: +91-40-27016168 Email: support@biotechdesk.com
CUSTOM ANTIBODY PRODUCTION

Polyclonal Antibodies
NIH 90-day standard protocol or 70-day conventional protocol 10 ml pre-immune serum 100 ml anti-serum
Monoclonal Antibodies
Free consultation for epitope selection Evaluation of screening methods Frequent interactions between You & Biotechdesk during hybridoma development process Project segmentation into different phases to provide flexibility for terminating the project at the end of each phase
Additional antibody services include:

ELISA Peptide synthesis 10 mg peptide, >80% purity, MS and HPLC certified Peptide-Carrier protein conjugation to BSA or KLH
Tel: +91-40-27017477/ 65916167
nts a i r a v agged t y t i n i s, Aff n i e t o r p Native

GTP Family of Signaling Proteins
Small GTPases (Ras Superfamily) Heterotrimeric G-Proteins GEFs (Guanine Nucleotide Exchange Factors) GAPs (GTPase Activation Proteins) GTPase Cycle Effectors GTPase Modifying Enzymes GPCRs (G-Protein Coupled Receptors) Importins
ucts d o r p ciated o s s A &
Serine/Threonine Kinases
MEK, MKK
Protein Kinase A, B and C

Protein Kinase A AkT/ PKB PKC-a, PKC-b, PKC-g, PKC-d, PKC-e Inhibitors Antibodies Substrates
Other Related Proteins:

Calcium/ Calmodulin-dependent kinases Cyclin dependent Kinases Phosphatase Transcription Factors
Phosphoinositide 3-kinase (PI3K)

PI3K a PI3K b PI3K g PI3K d Adaptor Proteins Substrates Inhibitors Antibodies
Biotech
Tel: +91-40-27017477 / 65916167 Fax: +91-40-27016168 Email: support@biodesk.com
Tyrosine Kinases
Abl, Hck, Lck, Src, PDGFR, Raf
Macromolecular Crystallography
The JBS Product Family- the key to protein structure determination
Crystallization Screens
What makes JBScreeen Classic so different from other crystal screens available? The composition of the buffers in the screen cover 240 of the most prominent reagent mixtures for protein crystallization. Their composition results from data mining several thousands of crystallized proteins.
JBScreen Classic bulk

10 individual kits, 24 conditions each, supplied in 10 ml tubes
JBScreen Classic ampoules

10 individual kits, 24 conditions each, supplied as 0.7 ml single shots in a vial
JBScreen Classic refill

Refill your JBScreen ampoules hits
JBScreen Classic HTS

2 pre-filled 96 deep-well Blocks
Wizard Screens I & II from Emerald Biosystems

Highly effective random sparse matrices for the crystallization of biologicalmacromolecules
TM
JBScreen Membrane
Cover 72 of the most promising reagents for crystallization of membrane proteins
JBScreen Cryo
Has been designed for vapour diffusion set-ups to determine initial crysallization conditions
JBScreen Cryo Pro

Produce effective cryo-protectants from your crystallization reservoir solution
JBScreen Plus
Most useful in the optimization of preliminary crystallization conditions
JBScreen Detergents kits

Are ideal supplements to the JBScreen Membrane screens
JBS Halo Kits

Halogenated ATP and GTP analogs provide an alternative method that allow incorporation of heavy atoms into a large number of physiologically relevant enzymes.
Ask us for plates and accessories for crystallization: Linbro plates, CombiClover Plates, Compact Clover plates, Sealing Tape, Cover Slides and Grease
Biotech Desk Pvt. Ltd. Tel: +91-40-27017477/ 65916167
Biotech
Fax: +91-40-27016168
ANTIBODIES FOR EVERYTHING!!

In Need of Phosphospecific Antibodies?
Biotechdesk offers a large selection of phosphospecific antibodies including:
Phosphospecific antibodies are available in biotinylated and dye-labeled forms, as well as AP/ HRP and APC/ B-PE/ R-PE conjugates, Biotechdesk also provides custom phosphospecific Antibody production; please contact support@biotechdesk.com for more information.
Antibodies for:
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Secondary Antibodies:
Tagged to Biotin, HRP , Alkaline Phosphatase, FITC, Texas Red, Cy3, PE, Cy5 or Gold
Cancer Neuroscience Signal transduction Cell cycle regulators Apoptosis Cytokines MMPs Viruses
Epitope Tagged Antibodies

Anti-His, Anti-HA, Anti-GST, Anti-GFP, Anti-cMyc and more
Substrates
OPD, TMB, DAB, Urea Peroxide, BCIP/NBT
Antibody Related Kits:

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KLH Conjugation BSA Conjugation Maleimide-activated Antigen Conjugation Alkaline Phosphatase Assay Peroxidase ELISA Assay Biotin Labelling Biotin, Streptavidin and its conjugates
If you cannot find what you are looking for, we will custom make the antibody for you!! Try our polyclonal and monoclonal custom antibody production services
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Biotech
A N T I B O D I E S
GENOMIC CLONING
Construct BAC or Fosmid Libraries in single copy and induce to High Copy number!
CopyControl Fosmid Library Production
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CopyControl BAC Library Production

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Cloning-ready pCC1FOS or pCC2FOS vector coupled to ultra high efficiency MaxPLax Lambda packaging extract No need for restriction enzyme digestion or PFGE to prepare DNA High cloning efficiencies
Linearized, dephosphorylated and highly purified pCC1 BAC vector Fast Link DNA Ligase which reduces ligation times from 16h to 4 h Unique colony screening process for estimating size of the BAC clones without restriction digestion or PFGE
Maximise yields from every clone!!

BACMAX and FosmidMAX DNA Purification Kit
Superior yields- alkaline lysis method Convenient- No columns, no organic extractions Versatile- Protocol can be scaled up from 1.5 ml to 100 ml
Electroporation Cuvettes
Our range of HiMaX Electroporation cuvettes are designed to maximise molecular electroporation and electrofusion efficiencies for Bacteria, Yeast, Insect, Plant and Mammalian cells.
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UNBEATABLE PRICE COMPATIBILITY The cuvettes are compatible with most electroporation systems LOW DEAD VOLUMES All 1mm and 2mm cuvettes have a tapered V bottom so that reduced sample volumes can be used while aiding sample pick up and minimizing dead volumes.
Optimizing Real-Time PCR

Using Labeled Probes
FailSafe PROBES Real-Time PCR Optimization Kit FailSafe PROBES Real-Time PCR PreMix-Choice Kit Convenient, simple & easy to use
PreMixes are preoptimized to save you time and energy.
Using SYBR Green I Dye

FailSafe GREEN Real-Time PCR Optimization Kit FailSafe GREEN Real-Time PCR PreMix-Choice Kit FIRST TIME
Perform real-time PCR with your template & primers using the FailSafe GREEN Real-time PCR Optimization Kit & select the optimal Real-time PCR Premix
Simple Optimization
Robust enzyme mix and set of 2X optimization PreMixes contains all necessary components necessary for successful PCR. Enables optimum amplification efficiency to generate high quality, trustworthy data.
And EVERY TIME

For consistent PCR results, choose the PCR Premix identified as optimal when you get the FailSafe GREEN Real-time PCR Premix Choice Kit Real time PCR Premixes contain all components for quantitative PCR using Sybr Green I dye. No specific probes required. Includes ROX, an internal reference dye. Kits available for both tube and capillary instruments
Consistent results
Enzyme and PreMix formulations do not change which gives you consistent, error-free results every time.
No hot-start needed
FailSafe PROBES Real-Time PCR System is compatible with all real-time PCR instruments and fluorescent probes.
MasterAmp GREEN Real-Time RT-PCR Kit

Realtime RT-PCR
The MasterAmp GREEN Real-Time RT-PCR Kit provides all necessary components to perform high-sensitivity one-step quantitative T-PCR using SYBR Green I Dye for detection.
Extremely high sensitivity and specificity. Easy-to-use PreMix contains all components for successful quantitative RT-PCR, including SYBR Green I dye. A thermostable enzyme increases sensitivity and specificity by reducing RNA secondary structure. MasterAmp PCR Enhancer increases sensitivity and specificity by reducing polymerase pausing and stops during reverse transcription and PCR. SYBR Green I dye for detection saves time and expense compared to use of labeled primers. The kit uses flexible protocols for real-time detection on almost all real-time PCR thermocyclers
Biotech Desk Pvt. Ltd. Tel: +91-40-27017477/ 65916167 Fax: +91-40-27016168 e-mail: support@biotechdesk.com
Perform PCR with your tem plate and primers usin g the FailSa fe PCR PreMix Selec tion kit and c hoose the PreMix with th e best amplific ation.
FIRST TIME
FailSafe
PCR System
Whatever the length, whatever the sequence, the FailSafe PCR System will faithfully amplify your template every time!
Applications
Amplify any template up to 20 kb l Amplify templates up to 85% GC rich l High sensitivity amp. of as little as 1 copy of template l High fidelity amplification l Multiplex PCR l No need for hot start
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Y TIME Get the sele cted PreMix with the FailSafe PC R System an d use it for c o n s is te n t a m p l if ic a ti o n of your template/prim er pair.
And EVER
MasterAmp High Sensitivity RT-PCR Kit

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MasterAmp High Fidelity RT-PCR Kit

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High sensitivity RT-PCR from as little as 1 pg of total cellular RNA Out-performs similar products from other vendors Reduces effect of RNA secondary structure by performing RT reactions at higher temperature Reduces chance for cross contamination by using one enzyme in a one-step and one-tube reaction protocol with no sample transfer MasterAmp PCR Enhancer Technology enhances PCR performance with GC-rich and other difficult templates
Highly accurate RT-PCR amplification of RNA templates for

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Cloning Sequencing Expression Transcription analysis
All purpose RT-PCR in which full-length amplification and high accuracy are essential Multiplex RT-PCR Comes along with an TAQURATE, a high flidelity polymerase enzyme blend
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Never-ending reasons why you should buy the MonsterScript 1st-Strand cDNA Synthesis Kit
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MonsterScript Reverse Transcriptase lacks RNase H, enabling improved synthesis of full-length cDNA even for long mRNA. MonsterScript is thermostable, permitting reverse transcription at temperatures >50C, reducing RNA secondary structure& improves priming specificity. The kit includes both an oligo(dT)-containing and a random nonamer primer. A potent RNase Inhibitor is included.
MonsterScript RT PreMix contains optimized concentrations of dNTPS, Mg+2 and Betaine for superior performance & minimal pipetting steps. Betaine in the cDNA Synthesis PreMix reduces pausing and stops during reverse transcription. First-strand cDNA can be made from picogram amounts of total RNA. The cDNA is a good template for subsequent end-point PCR or real-time PCR.
Tel: +91-40-27017477/ 65916167
Fax: +91-40-27016168
Purify DNA from Buccal Cells/any sample

QuickExtract DNA Extraction
The QuickExtract DNA Extraction Solution permits processing of samples using a simple protocol. Aliquots of the QuickExtract Solution are provided as individual sample tubes. To obtain PCR-ready DNA, just rotate the buccal sample swab in one of these tubes, mix, and heat. No centrifugation step is needed; so sample-handling times are very short.

BuccalAmp DNA Extraction Kit
Cat. No. QE09050
Product QuickExtract DNA Extraction Solution 1.0
Qty 50 ml Figure 1. BuccalAmp extraction protocol.
Catch-All Sample Collection Swabs

Soft foam swabs on soft, flexible plastic handles. Catch-All Swabs provide gentle, safe buccal sample collection, even for infants, and the porous foam on these swabs catches more of the sample than buccal brushes. Catch-All Swabs, also available separately, are provided individually packaged in sterile hard-pack plastic cylinders. After collecting the sample, return the sample swab to the cylinder package, for safe, secure storage and transport from the collection site to the analysis site. Versatilerecover DNA from the leaves of many plant species, even those high in polysaccharides and polyphenolic compounds. Rapidpurify DNA in less than one hour. Yields high molecular weight DNA. Simple and non-toxiceliminates organic solvent and CTAB extractions, CsCl gradients, and enzyme digestions. Recover DNA of higher integrity than column-based purification methods.
Cat. No. BQ0901S BQ0908S BQ0916S
Products BuccalAmp DNA Extraction Kits BuccalAmp DNA Extraction Kits BuccalAmp DNA Extraction Kits
Qty 1 kit (15 rxns) 8 kits 16 kits
MasterAmp Buccal Swab Kits and DNA Extraction Solution

The MasterAmp Buccal Swab DNA Extraction Kits are easy-to-use, singletube systems for the rapid preparation of DNA from human or other mammalian buccal (cheek) cells for PCR amplification. The format allows processing of one to hundreds of samples in less than one hour. Yields range from 0.5-3.0 g of DNA from a buccal sample, enough to perform several PCR amplifications. The standard MasterAmp Buccal Swab DNA Extraction Kit contains individually packaged Buccal Swab Brushes in a sterile, soft package. The kit for remote site testing contains brushes for off-site use that are packaged in sterile, hard-pack plastic cylinders for secure storage and transport from the collection site to the analysis site. Benefits No blood draws are necessary to obtain PCR-ready DNA. Simple and rapid extraction protocol. Includes MasterAmp PCR Enhancer Technology, which improves many PCR amplifications. Single-tube system assures sample integrity and prevents contamination. Safeuses no organic solvents.
Cat. No. QEC091H QEC0925
Product Catch-All Sample Collection Swabs Catch-All Sample Collection Swabs
Qty 100 swabs 25 swabs
BuccalAmp DNA Extraction Kit

The BuccalAmp DNA Extraction Kit is a single-tube system for rapid preparation of DNA from buccal (cheek) swab samples for use in PCR amplification assays. The kit incorporates the QuickExtract DNA Extraction Solution, developed by EPICENTRE scientists, that permits processing of samples using a simplified new protocol. Benefits Simple and rapid sample collectiona swab of the inner cheek replaces blood draws for obtaining genomic DNA. Gentlethe sample is collected using a swab made of soft, porous foam on a soft, flexible plastic handle. Simple and rapid DNA extraction protocol - just rotate the swab sample in the QuickExtract Solution, mix, and heat. Safereduces risks from sample collection (e.g., needle sticks) and uses no phenol, chloroform, or other toxic solvents. Amenable to automation and high throughputno centrifugation needed.
Cat. No. MB71030
Product MasterAmp Buccal Swab DNA Extraction Kit (standard)* MasterAmp Buccal Swab Brushes (standard)** MasterAmp Buccal Swab DNA Extraction Kit (remote site testing)* MasterAmp Buccal Swab Brushes (remote site testing)*** MasterAmp DNA Extraction Solution
Qty
30 rxns
MB030BR
30
MB79100
100 rxns
MB100BR
100 50ml (100 rxns)
MB7901S
*Contents : MasterAmp DNA extraction solution, Swab brushes ** Individually packed sterile brushes in soft pack *** Individually packed sterile brushes in hard pack
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DNA Purification from Blood, Plant leaf, Soil, Water and Feaces
MasterPure DNA Purification Kit for Blood
High yield of genomic DNAup to 10 g from 325 l of whole blood and approximately 100 g from 600 l of buffy coat. High DNA purityA260/280 ratios of 1.8-2.0. Rapidcan be completed in less than 30 minutes. Convenientthe buffy coat protocol uses only 1.5-ml microcentrifuge tubes and no columns. Safeno organic solvents are used. Purified DNA is PCR-ready. 5 ml blood samples can be centrifuged to generate 600 l of buffy coat per sample. Each 600ul of buffy coat yield about 100ug DNA on an average.

SoilMaster DNA Extraction Kit

The kit utilizes a hot detergent lysis process combined with a simple chromatography step that removes organic inhibitors, such as humic and fulvic acids, that are known to co-extract with DNA from soil and sediment samples. The SoilMaster Kit isolates larger and more intact DNA than other soil DNA kits incorporating bead beating or vortex mixing. Benefits
Rapidextract PCR-ready soil DNA in less than 45 minutes. Simpleno need for dedicated equipment or bead beating. Larger, higher molecular weight DNA recovery. Humic acid and protein removal Product SoilMaster DNA extraction kit Spin Columns Inhibitor Removal resin Qty 50 rxns 50 Columns 55ml
Cat. No. SM0250
Cat. No. MG71100
Product MasterPure DNA Purification Kit for Blood
Qty 100 rxns
SC04350 SR04350
MasterPure Plant Leaf DNA Purification Kit

Versatilerecover DNA from the leaves of many plant species, even those high in polysaccharides and polyphenolic compounds. Rapidpurify DNA in less than one hour. Yields high molecular weight DNA. Simple and non-toxiceliminates organic solvent and CTAB extractions, CsCl gradients, and enzyme digestions. Recover DNA of higher integrity than column-based purification methods. Product MasterPure Plant Leaf DNA Purification Kit MasterPure Plant Leaf DNA Purification Kit Qty 10 rxns
WaterMaster DNA Purification Kit

The WaterMaster DNA Purification Kit provides all of the liquid reagents needed to purify microbial DNA from any water source after passage through a filter of your choice. This capability includes DNA from bacteria and eukaryotes. Benefits Simple, gentle procedure employs no toxic reagents or mechanical disruption Can detect DNA from bacteria as low as 10 per 100 ml Small final volume of DNA 50-fold lesser than competing kits
Cat. No. MPP92010
Cat. No. WM04005
Product WaterMaster DNA Purification Kit WaterMaster DNA Purification Kit
Qty 5 rxns 20 rxns
MPP92100
100 rxns
WM04020
MasterPure Gram Positive DNA Purification Kit

The MasterPure Gram Positive DNA Purification Kit provides all of the reagents needed to purify DNA from gram positive bacteria. These bacteria lyse more readily after treatment with Ready-Lyse and the Gram Positive Cell Lysis Solution. Ready-Lyse Lysozyme is a stable solution of a non-mammalian, non-avian recombinant lysozyme, with high specific activity and no net charge at neutral pH. Thus, there is no waiting to dissolve the lysozyme and it does not bind DNA. Some of the species it has been shown to work on are: Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Lactococcus lactis Cat. No. MGP04020 Product MasterPure Gram Positive DNA Purification Kit MasterPure Gram Positive DNA Purification Kit Qty
ExtractMaster Fecal DNA Extraction Kit

The ExtractMaster Fecal DNA Extraction Kit provides all of the reagents necessary to recover PCR-ready DNA from the feces of a variety of animals. The kit utilizes a detergent lysis process combined with a chromatography step, which removes enzymatic inhibitors that often coextract with DNA from such samples. The extracted DNA is PCR-ready, and can be used with the FailSafe PCR System to amplify bacterial, protist or animal templates. Benefits Simple, gentle procedure no toxic reagents or mechanical disrution Small final volume of DNA Product ExtractMaster Fecal DNA Extraction Kit ExtractMaster Fecal DNA Extraction Kit Qty 5 rxns 25 rxns
Cat. No. 20rxns FD05005 100rxns FD05025
MGP04100
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Isolate DNA/RNA/mRNA from any sample

MasterPure Complete DNA and RNA Purification Kits MasterPure DNA Purification Kit MasterPure RNA Purification Kit
MasterPure DNA & RNA Purification Kits use a novel technology that enables efficient purification of intact total nucleic acid (TNA), DNA, or RNA from every type of biological material (Table1). Targets Human & Mammalian RNA & Genomic DNA HIV E. coli HCV B. pertussis RSV Yeast M. tuberculosis Enterovirus HPV Soy (tofu) Maize Insect tissues Streptococcus mutans Benefits Extensive sample rangePurify DNA or RNA from any sample, every time. Quick, easy protocolpurify TNA in 30 minutes, DNA in 45 minutes, and RNA in 60 minutes, without cumbersome columns. High purityO.D. 260/280 ratios consistently between 1.8 and 2.0. High yields e.g. recover >90% of theoretical DNA yield from E. coli . High sensitivitycompletely recover even small amounts of nucleic acid Safeno phenol, chloroform, or other caustic solvents. Samples Extracted Serum Plasma Whole blood Guthrie cards Buccal cells Liver Mouse tail Kidney Saliva Urine Sputum Tissue culture cell lines Cervical cells Paraffin-embedded tissues

MasterPure Individually Packaged, Bulk Reagents (10X Size)

Cat. No. MRC0912H MTC096H MMP095H MMP03750 MTE0970 MTC085H MPRK092 D9905K MBD092H MRNA092 MPS09250 PCR04050 PCR04250 Product Red Cell Lysis Solution Tissue & Cell Lysis Solution MPC Protein Precipitation Solution MPC Protein Precipitation Solution TE Buffer 2X T&C Lysis Buffer Proteinase K 50 g/l DNase I, RNase-Free 1 U/l 1X DNase Buffer RNase A 5 g/l Precipitation Solution for Blood PCR Precipitation Solution PCR Precipitation Solution Qty 1200 ml 600 ml 500 ml 50 ml 70 ml 500 ml 2 ml 5 ml 200 ml 2 ml 250 ml 50 x 50 l Purif. 250 x 50 l Purif.
mRNA-ONLY Prokaryotic mRNA Isolation Kit mRNA-ONLY Eukaryotic mRNA Isolation Kit
For isolation of rRNA-free prokaryotic/eukaryotic mRNA The mRNA-ONLY Prokaryotic mRNA Isolation Kit provides a novel, simple and effective method for obtaining mRNA transcripts that are substantially free of ribosomal RNA (rRNA) in about an hour. Although oligo(dT)- or oligo(dU)- immobilized polymers or other substrates are commonly used to purify polyadenylated eukaryotic mRNA, this has not been possible for prokaryotic mRNA since most prokaryotic mRNA is not polyadenylated. The mRNA-ONLY protocol begins with a purified preparation of total RNA from a prokaryote, such as that obtained using the MasterPure RNA Purification Kit. The first step of an mRNA-ONLY protocol is treatment of total RNA with a proprietary mRNA-ONLY Isolation Solution, which removes 16S and 23S prokaryotic rRNA in the preparation, leaving a solution that is highly enriched for mRNA. In some protocols, mRNA is then salt-precipitated using the LiCl Solution provided in the kit. Cat. No. Product mRNA-ONLY Prokaryotic mRNA Isolation Kit mRNA-ONLY Prokaryotic mRNA Isolation Kit mRNA-ONLY Eukaryotic mRNA Isolation Kit mRNA-ONLY Eukaryotic mRNA Isolation Kit Qty 10 purif. 24 purif. 10 purif. 24 purif.
Cat. No. MC89010 MC85200 MCD85201 MCR85102
Product Complete DNA & RNA Purification Kit* Complete DNA & RNA Purification Kit* MasterPure DNA Purif. Kit MasterPure RNA Purif. Kit
Qty 10 rxns 200 rxns 200 rxns 100 rxns
MOP51010 *Contents: Red Cell Lysis Solution,Tissue and Cell Lysis Solution, MPC Protein Precipitation Reagent, 2X T&C Lysis Solution, TE Buffer, RNase A, Dnase I, RNase-Free Proteinase K, 1X DNase Buffer. The DNA Purification kit has only RNAse A and no DnaseI The RNA Purification kit has only DnaseI and no Rnase. MOP51024 MOE51010 MOE51024
Contents: Proprietary mRNA-ONLY mRNA Isolation Reagents, including mRNA-ONLY Isolation Solution, RNase Inhibitor, 10X mRNA-ONLY Isolation Buffer, Stop Solution, and LiCl Precipitation Solution.
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Purify DNA/RNA from Yeast, BACs, Fosmids and for Microarray

MasterPure Yeast DNA Purification Kit
The MasterPure Yeast DNA Purification Kit enables efficient, high-yield purification of high molecular weight DNA from yeast and other fungi. The protocol involves non-enzymatic cell lysis at 65C, followed by removal of protein by precipitation, then nucleic acid precipitation and resuspension. No lyticase or proteolytic enzymes are used in the procedure. Higher yields of yeast chromosomal DNA than with other kits (Figure 1). Versatilerecover DNA from a wide variety of yeast species including Candida , Saccharomyces , Pichia , and Schizosaccharomyces, and filamentous fungi such as Aspergillus 2 and Penicillium . Rapidpurify DNA in less than 40 minutes. Simple and safeeliminates the need for enzymatic lysis, columns, and phenol or other organic extractions.

ArrayPure Nano-scale RNA Purification Kit

The ArrayPure Nano-scale RNA Purification Kit provides all of the reagents needed to purify RNA from one to a few thousand eukaryotic cells, such as obtained from Laser Capture Microdissection (LCM) procedures.The reagents are all aqueous to avoid the use of toxic organic solvents.This nano-scale protocol has been developed and tested with quantitative real-time PCR on 1 to 10,000 eukaryotic cells. Cell numbers were based on living cells that were enumerated and then diluted. The average yields of RNA from 1,000 and 10,000 HeLa cells, as determined by RiboGreen fluorescence, were 21 and 213 ng, respectively. In addition, g amounts of RNA have been produced from 20 HeLa cells using aRNA 2round synthesis techniques. Applications: RT-PCR, aRNA, LCM Cat. No. MPS04050 Product ArrayPure Nano-scale RNA Purification Kit Qty 50 Purif.
BACMAX DNA Purification Kit FosmidMAX DNA Purification Kit

The BACMAX / FosmidMAX DNA Purification Kit was developed for easy, reliable isolation of high-quality BAC/Fosmid DNA. The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6 to 25 g of BAC/Fosmid DNA from 1.5 to 100 ml of a single-copy BAC culture. Selective precipitation steps and the incorporation of EPICENTREs RiboShredder RNase Blend effectively remove contaminants that degrade DNA and interfere with downstream applications. Figure 1. The MasterPure Yeast DNA Purification Kit gives higher yields of DNA than other kits. DNA was quantitated specifically with Hoechst fluorescent dye 33258, which gives minimal fluorescence with RNA. Cat. No. MPY80010 MPY80200 Product MasterPure Yeast DNA Purif. kit MasterPure Yeast DNA Purif. kit Qty 10 rxns 200 rxns Cat. No. Product BACMAX DNA Purification Kit* FosmidMAX DNA Purification Kit Qty 1 kit 1 kit Higher yields of BAC DNA than with other kits. Superior quality Isolate BAC DNA pure enough to store for one year at -20C without being degraded. Versatile sample size Easy to follow protocols for isolating up to 0.6, 4, 11, or 25 g of BAC DNA from 1.5, 10, 40, or 100-ml cultures of a single-copy BAC, respectively. Convenient Eliminate the need for columns, resins, or organic extractions.
MasterPure Yeast RNA Purification Kit

The MasterPure Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from cell types including: Candida, Saccharomyces, Schizosaccharomyces , and filamentous fungi. The kit utilizes a rapid desalting process to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting. Avoid hot acid phenol Faster than spheroplasting Higher quality than bead-beating No extra enzyme or equipment to purchase RNA can be used for microarray Yields 25-50 ug RNA from 1 ml of mid log S. cerevisiae
BMAX044 FMAX046
Reagents sufficient for 150 purifications with 1.5 ml; 30 purifications with 10 ml; 10 purifications with 40 ml; or 5 purifications with 100 ml.
PlasmidMAX
For isolation of plasmids, please view page # 16
GELase
Extraction of DNA from Gels, please see page # 17
Cat. No. MPY03010 MPY03100
Product MasterPure Yeast RNA Purif. kit MasterPure Yeast RNA Purif. kit
Qty 10 rxns 100 rxns
! T : 040-27017477 / 65916167
F : 040-27016168
PCR PRODUCT SELECTION GUIDE

FailSafe PCR System AmpliTherm DNA Polymerase MasterAmp ExtraLong PCR Kit Taq DNA Polymerase Tfl DNA Polymerase Tth DNA Polymerase
PCR
Polymerase Chain Reaction
Properties of PCR products

5 3 structure dependent exonuclease 3 5 proofreading exonuclease Terminal transferase activity a Reverse transcriptase activity c, d Amplify < 3 kb DNA Amplify 3-5 kb DNA Amplify 5-15 kb DNA Amplify 15-20 kb DNA Amplify 40 kb DNA
The FailSafe PCR System, the MasterAmp Extra-Long PCR Kit, and the FailSafe Real-Time PCR System require no additional optimization components. For other thermostable DNA polymerases, EPICENTRE offers two different PCR optimization kits. The proper optimization kit should be chosen based upon the polymerase used. The following table defines the appropriate kit to use for each thermostable enzyme.
+ + +b + + + + -
+ + + + + + +
+ + + + + -
+ + + + + -
+ + + + + -
+ + + -
PCR Optimization Kits
MasterAmp PCR Optimization Kit
MasterAmp PCR Optimization Kit with Ammonium Sulfate
MasterAmp Taq DNA Polymerase MasterAmp AmpliTherm DNA Polymerase MasterAmp Tfl DNA Polymerase MasterAmp Tth DNA Polymerase
Whatever the template, whatever its sequence, the FailSafe PCR System is almost always your best choice for dependable amplification of any template up to 20 kb every time. If the template is larger than 20 kb, use the MasterAmp Extra-Long PCR Kit. For real-time quantitative PCR applications, use the FailSafe Real-Time PCR System. a b 3-uncoded nucleotide addition (e.g., 3-terminal A overhang). PCR products synthesized using the FailSafe PCR System can be cloned with good cloning efficiencies using both blunt-end vectors and vectors with a 5-terminal T overhang. Mn 2+-dependent. Note, Taq and Tfl DNA polymerases have been reported to catalyze Mn 2+-dependent reverse transcription. However, the level of RT activity is significantly less than that of RetroAmp RT and Tth DNA Polymerases; therefore we do not recommend Taq or Tfl DNA Polymerase for reverse transcription.
c d
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FailSafe and MasterAmp PCR Optimization Kits

FailSafe PCR System
The FailSafe PCR System is the only PCR system that ensures successful PCR, the first time and every time. It provides accuracy, consistency, and dependability for PCR, regardless of the source or the property of the DNA templates. The system includes the FailSafe PCR Enzyme Mix, an extensively tested set of 12 FailSafe PCR PreMixes, representing various PCR conditions. Along with the FailSafe Enzyme Mix, these PreMixes contain everything you need for a successful PCR: dNTPs, buffer, and varying amounts of MgCl 2 and FailSafe PCR Enhancer (with betaine). The FailSafe PCR Selection kit has replaced the need for cumbersome adjustments of reaction components to determine optimal conditions of PCR of each template and primer pair. The 12 FailSafe PCR Premixes in this kit cover a meticulously determined matrix of PCR conditions that give optimal PCR results for different sequences. FIRST TIME Perform PCR with your template and primers using the FailSafe PCR PreMix Selection Kit and choose the PreMix that provides the best amplification. Applications Amplify any template up to 20 kb Amplify templates up to 85% GC rich High sensitivity amplification of as little as 1 copy of template High fidelity amplification Multiplex PCR No need for hot start Product FailSafe PCR PreMix Selection Kit * FailSafe PCR System FailSafe PCR System FailSafe PCR System Failsafe Enzyme Mix only Failsafe Enzyme Mix only Qty 1 kit (60U) 1 kit (100U) 1 kit (250U) 1 kit (1000U) 100U 1000U MO751N EVERY TIME Get the selected PreMix with the FailSafe PCR System and use it for consistent amplification of your template/ primer pair.
PCR
PCR Optimization
MasterAmp PCR Optimization Kits

MasterAmp PCR Optimization Kits are compatible with thermostable enzymes frequently used in PCR, such as Taq, Pfu, Tth, and Tfl DNA polymerases. You can significantly improve the performance of PCR systems in one experiment by adding your own enzyme, template, and PCR primers into the PreMixes supplied with the kit. The MasterAmp PCR Optimization Kits contain a series of 12 MasterAmp PCR 2X PreMixes that have been meticulously tested and represent a complete range of PCR conditions frequently encountered. Each MasterAmp 2X PreMix contains all components needed for PCR except for the primers, the template, and the PCR enzyme. These components include dNTPs, buffer, and varying amounts of MgCl 2 and the MasterAmp PCR Enhancer (with betaine). Choice of Optimization Kit There are two types of MasterAmp PCR Optimization Kits available, with and without ammonium sulfate. Choosing the appropriate kit is dependent upon the type of thermostable enzyme used in PCR, since ammonium sulfate is important to the optimal enzymatic activity of certain enzymes and detrimental to that of others. Cat No. MOS001 MO7201 MOS02N Product MasterAmp PCR Optimization Kit MasterAmp PCR Optimization Kit MasterAmp PCR Opti. Kit with Amm. sulfate MasterAmp PCR Opti. Kit with Amm. sulfate Qty. 20 rxns 60 rxns 20 rxns 60 rxns
MO7205A or MO7205AN MO7205L or MO7205LN are the catalogue numbers of the 12 different MasterAmp PCR 2X PreMixes to be used with the Optimization kit. The product numbers ending in N contain ammonium sulfate. Cat No. MO7205A (N)-L (N) Product MasterAmp PCR 2X PreMix Qty. 5 ml
Cat. No. FS99060 FS99100 FS99250 FS9901K FSE51100 FSE5101K
MasterAmp Extra-Long PCR Kit

The MasterAmp Extra-Long PCR Kit is designed for accurately amplifying DNA sequences up to about 40 kb. The MasterAmp ExtraLong DNA Polymerase Mix is an enzyme mixture, combining MasterAmp Taq DNA Polymerase with a 35 proofreading enzyme, to achieve PCR fidelity at least three times higher than Taq DNA polymerase alone. Included in the kit are MasterAmp Extra-Long DNA Polymerase Mix, a set of 9 pre-optimized Extra-Long PCR 2X PreMixes, and a control template consisting of a mixture of lambda DNA and primers. The set of 9 ExtraLong PreMixes represents a complete range of extra-long PCR conditions. Each PreMix contains dNTPs, buffer, and varying amounts of MgCl2 and the MasterAmp PCR Enhancer (with betaine). Cat. No. MHF9220 QU92125 Product MasterAmp Extra-Long PCR Kit MasterAmp Extra-Long PCR DNA Polymerase Mix MasterAmp Extra-Long PCR DNA Polymerase Mix MasterAmp Extra-Long PCR DNA Polymerase Mix MasterAmp Extra-Long PCR 2X Premixes 1-9 Qty 50 rxns 125U 500U 1000U 5 ml each
*Contains FailSafe PCR Enzyme Mix and the 12 FailSafe PCR 2X PreMixes. Note: Each FailSafe PCR System contains FailSafe PCR Enzyme Mix and a choice of FailSafe PCR 2X PreMixes (any combination; choose below ): Cat No. FS99100 includes a choice of one PreMix. Cat.No. FS99250 includes a choice of two PreMixes. Cat.No. FS9901K includes a choice of eight PreMixes.
The PreMixes are also available for purchase separately:
QU92500 Qty 2.5 ml QU9201K MHF925A-I
Cat. No. FSP995A-L
Product FailSafe PCR 2X PreMix
MHF925A MHF925I are the catalogue numbers of the 9 different MasterAmp Extra-Long 2X PreMixes to be used with this kit.
! T : 040-27017477 / 65916167
F : 040-27016168
Thermophilic & Mesophilic DNA Polymerases

Properties of Mesophilic DNA Polymerases
Activity Product Name 5'3' 3'5' exonuclease exonuclease Nick translation Heat Inactivation* 75C 20 minutes 75C 20 minutes 75C 20 minutes 65C 10 minutes 75C 20 minutes 75C 20 minutes Strand displacement
PCR
Polymerases
DNA Polymerase I, E. coli Klenow DNA Polymerase Exo-Minus Klenow DNA Polymerase RepliPHI Phi29 DNA Polymerase
+ -
++ ++ ++ +++ +++
+ Activity
+ + ++++ -
T4 DNA Polymerase T7 DNA Polymerase, Unmodified
Properties of Thermophilic DNA Polymerases

Product Name
Reverse 5'3' transcriptase exonuclease
3'5' exonuclease
Thermostability b <15 min at 75C <1.5 min at 95C
Fidelity C N/A
Strand displacement
rBst DNA Polymerase a rBst DNA Polymerase Large Fragment, (IsoTherm DNA Polymerase) a MasterAmp AmpliTherm DNA Polymerase MasterAmp Taq DNA Polymerase MasterAmp Tfl DNA Polymerase MasterAmp Tth DNA Polymerase
+ Very weak Requires Mn 2*
+ + + + + +++
+++
<15 min at 75C <1.5 min at 95C
N/A
N/A
N/A
N/A
9 min at 97.5C 40 min at 95C
0.38-1.82 x 10 4 8.3.9.0 x x 10 5 2.2 x 10 4
N/A N/A
++
Requires Mn 2*
20 min at 95C
N/A
rBst DNA Polymerase

rBst DNA Polymerase, produced from an overexpressing recombinant clone in E. coli , is the product of the DNA pol I gene of the thermophilic bacterium Bacillus stearothermophilus (Bst). The enzyme has optimal activity at 65C and at elevated temperatures it can synthesize DNA in regions containing template secondary structure or high GC content where other non-thermostable DNA polymerases may fail. The enzyme has 5' 3' exonuclease activity. rBst DNA Polymerase is also active as a thermostable RNA-dependent DNA polymerase (reverse transcriptase). Cat. No. BH1100 BH1500 BH101K BL901K Product rBst DNA Polymerase (5 U/l) rBst DNA Polymerase (5 U/l) rBst DNA Polymerase (5 U/l) rBst DNA Polymerase, Large Fragment (IsoTherm DNA Polymerase) (5 U/l) rBst DNA Polymerase, Large Fragment (IsoTherm DNA Polymerase) (50 U/l) rBst DNA Polymerase, Large Fragment (IsoTherm DNA Polymerase) (50 U/l) Qty 100 U 500 U 1,000 U 1000 U
rBst DNA Polymerase, Large Fragment (IsoTherm DNA Polymerase)

rBst DNA Polymerase Large Fragment is the product of the DNA pol I gene of the thermophilic bacterium Bacillus stearothermophilus (Bst) altered to remove the 5' 3' DNA exonuclease activity. Like the Klenow fragment of E. coli DNA Polymerase I, rBst DNA Polymerase Large Fragment has strong strand displacement activity. It also has thermostable reverse transcriptase activity.
BL1805K
5000 U
BL1950K
50,000 U
! T : 040-27017477 / 65916167
F : 040-27016168
Thermophilic and Mesophilic DNA Polymerases

Thermophilic DNA Polymerases (~ 95 C)
These have optimal DNA synthetic activity above 70 C and can be used up to 90 C. These are the following: Cat. No. Q82100 Q82250 Q82500 Q8210K Q8250K Product MasterAmp Taq DNA Polymerase (5 U/l) MasterAmp Taq DNA Polymerase (5 U/l) MasterAmp Taq DNA Polymerase (5 U/l) MasterAmp Taq DNA Polymerase (5 U/l) MasterAmp Taq DNA Polymerase (5 U/l)
PCR
Polymerases
Qty 100 U 250 U 500 U 1000 U 5000 U
MasterAmp AmpliTherm DNA Polymerase

It lacks both the 5'3' structure-dependent exonuclease activity like that found in Taq DNA polymerase and the 3' 5' proofreading exonuclease activity found in some other DNA polymerases.
MasterAmp Tfl DNA Polymerase

Derived from the thermophilic bacterium Thermus flavus , MasterAmp Tfl DNA Polymerase is a thermostable DNA polymerase useful for performing PCR. EPICENTREs MasterAmp Tfl DNA Polymerase has been shown to produce large PCR products (15 kb).
The buffers and enzyme are available separately also: Cat. No. FB3715 FB3760 AT72250N F72250N TTH7225N Q82250N Product MasterAmp Tfl 20X PCR Buffer MasterAmp Tfl 20X PCR Buffer MasterAmp AmpliTherm DNA Polymerase (5 U/l) (enzyme only) MasterAmp Tfl DNA Polymerase (1 U/l) (enzyme only) MasterAmp Tth DNA Polymerase (enzyme only) (5 U/l) MasterAmp Taq DNA Polymerase (5 U/l) (enzyme only) Qty 1.5 ml 5 ml 250 U 250 U 250 U 250 U
MasterAmp Tth DNA Polymerase

MasterAmp Tth DNA Polymerase is a thermostable DNA polymerase derived from the thermophilic bacterium Thermus thermophilus . The enzyme also has thermostable reverse transcriptase activity, which enables synthesis of cDNA from RNA templates. The high reaction temperatures possible with MasterAmp Tth DNA Polymerase minimize problems associated with nonspecific priming and template secondary structure.
MasterAmp Taq DNA Polymerase

MasterAmp Taq DNA Polymerase is a thermostable DNA polymerase derived from Thermus aquaticus . The enzyme has an intrinsic 5' 3' structure-dependent exonuclease activity, but lacks 3' 5' proofreading exonuclease activity. Cat. No. AT72100 AT72250 AT72500 AT7201K AT7205K F72100 F72250 F72500 F7201K F7205K TTH72100 TTH72250 TTH72500 TTH7201K TTH7205K Product Qty
Mesophilic DNA Polymerases DNA Polymerase I, E. coli

DNA Polymerase I from E. coli is a DNA-dependent DNA polymerase. The enzyme also contains both 5'3and 3' 5' exonuclease activities. The 5' 3' exonuclease activity enables the enzyme to use nicks and gaps in the DNA as starting points for labeling the DNA by nick translation.
MasterAmp AmpliTherm DNA Polymerase (5 U/l) 100 U MasterAmp AmpliTherm DNA Polymerase (5 U/l) 250 U MasterAmp AmpliTherm DNA Polymerase (5 U/l) 500 U MasterAmp AmpliTherm DNA Polymerase (5 U/l) 1000 U MasterAmp AmpliTherm DNA Polymerase (5 U/l) 5000 U MasterAmp Tfl DNA Polymerase (1 U/l) MasterAmp Tfl DNA Polymerase (1 U/l) MasterAmp Tfl DNA Polymerase (1 U/l) MasterAmp Tfl DNA Polymerase (1 U/l) MasterAmp Tfl DNA Polymerase (1 U/l) MasterAmp Tth DNA Polymerase (5 U/l) MasterAmp Tth DNA Polymerase (5 U/l) MasterAmp Tth DNA Polymerase (5 U/l) MasterAmp Tth DNA Polymerase (5 U/l) MasterAmp Tth DNA Polymerase (5 U/l) 100 U 250 U 500 U 1000 U 5000 U 100 U 250 U 500 U 1000 U 5000 U
Klenow DNA Polymerase

Klenow DNA Polymerase is derived from E. coli DNA polymerase I. This large fragment, DNA-dependent enzyme has 5' 3' polymerization and 3'5' exonuclease activities, but lacks 5'3' exonuclease activity. Klenow DNA polymerase blunt ends doublestranded DNA with singlestranded overhangs. The 3'5' exonuclease activity removes 3' overhangs and the 5'3' polymerization activity fills in 5' overhangs.
Klenow DNA Polymerase Fragment ExoKlenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase that lacks both the 5' 3' and 3' 5' exonuclease activities of E. coli DNA Polymerase I from which it is derived.
Cat. No. DP02250 DP021K DP0415K
Product DNA Polymerase I, E. coli (10 U/l) DNA Polymerase I, E. coli (10 U/l) DNA Polymerase I, E. coli (10 U/l) Klenow DNA Polymerase Klenow DNA Polymerase Fragment Exo
-
Qty 250 U 1000 U 5000 U 1000 U 1000 U
MasterAmp Taq PCR Core Kit

Cat. No. MCQ74200 Product MasterAmp Taq PCR Core Kit Qty 250 rxns
KP04061K KL04011K
Contents: MasterAmp Taq DNA Polymerax, IOX PCR buffer, IOX PCR Enhancer, dNTP Mix 2.5 mm each, 25 mm MgCl 2, Enzyme dilution buffer Control Template and Primers Mix
! T : 040-27017477 / 65916167
F : 040-27016168
Mesophilic DNA Polymerases

RepliPHI Phi29 DNA Polymerase
RepliPHI Phi29 DNA Polymerase (f29 DNA Polymerase) is a highly processive enzyme with exceptional strand displacement activity. The enzyme also contains a 3' 5' exonuclease activity that enables proofreading capability. Cat. No. PP031010 Product RepliPHI Phi29 DNA Polymerase (enzyme only) 1 g/l (1000 U/l)) RepliPHI Phi29 DNA Polymerase (enzyme only) 0.1 g/l (100 U/l) RepliPHI Phi29 Reagent Set (Enzyme: 1 g/l (1000 U/l)) RepliPHI Phi29 Reagent Set (Enzyme: 0.1 g/l (100 U/l)) RepliPHI Phi29 Polymerase Dilution Buffer Qty 10 g (10,000 U) Cat. No. D0602H D0605H D07250 D07500 D0701K Product T4 DNA Polymerase T4 DNA Polymerase T7 DNA Polymerase, Unmodified (10 U/l) T7 DNA Polymerase, Unmodified (10 U/l) T7 DNA Polymerase, Unmodified (10 U/l)
PCR
Polymerases
Qty 200 U 500 U 250 U 5000 U 1000 U
* includes enzyme, buffer, dNTPs, DTT
PP040110
10 g (10,000 U)
Other Polymerases for PCR

Hot Start Pol is recommended for high specificity PCR reactions and shows superior amplification at lowest template concentrations. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The polymerase contains monoclonal antibodies, which block polymerase activity prior to the onset of thermal cycling. It catalyzes the polymerization of nucleotides into duplex DNA in 5' 3' direction in the presence of magnesium. It also possesses a 5' 3' polymerizationdependent exonuclease replacement activity but lacks a 3'5' exonuclease activity. High Fidelity Pol is a blend of DNA polymerases specially designed for highly accurate and efficient amplification of fragments up to 5 kbp including GC-rich or other difficult templates. The enzyme blend includes a highly processive 5' 3' DNA polymerase and shows no detectable 5' 3' exonuclease activity. Its inherent 3'?5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq. Long Range Pol is a blend of DNA polymerases specially designed for highly accurate and efficient amplification of fragments up to 30 kbp. The enzyme blend includes a highly processive 5'3' DNA polymerase and shows no detectable 5'3' exonuclease activity. Its inherent 3'5' exonuclease proofreading activity results in a greatly increased length of amplification product compared to Taq. Sequencing Pol is a Taq polymerase mutant showing an enhanced efficiency for incorporation of ddNTPs. Its capability of incorporating ddNTPs and dNTPs at equal rates makes it the ideal choice for DNA cycle sequencing. The enzyme replicates DNA at 74C. It catalyzes the polymerization of nucleotides into duplex DNA in 5' 3' direction in the presence of magnesium and has a 5' 3' polymerisation-dependent exonuclease replacement activity only. Cat. No. PCR-103L PCR-103S Product Hot Start Master, L pack Hot Start Master, S pack, Master mix of thermally activated DNA polymerase for high specificity Hot Start Pol, L pack Hot Start Pol, S pack, Thermally activated DNA polymerase for high specificity High Fidelity Master, L pack High Fidelity Master, S pack, Master mix of thermostable DNA polymerase for high accuracy High Fidelity Pol, L pack High Fidelity Pol, S pack, Thermostable DNA polymerase for high accuracy Long Range Pol, L pack, Thermostable DNA polymerase for amplification of long templates Sequencing Pol Taq Pol mutant for incorporation of ddNTPs Sequencing Pol Taq Pol mutant for incorporation of ddNTPs Qty 500 rxns 100 rxns
RH031110
Enzyme: 10 g (10,000 U) Enzyme: 10 g (10,000 U)
RH040210
RPB04041
1 ml
T4 DNA Polymerase
T4 DNA Polymerase has both a template-directed 5' 3' DNA polymerase activity and a potent 3' 5' exonuclease activity. These characteristics make the enzyme useful for several molecular biology applications. Applications Conversion of both 5'- and 3'-protruding DNA termini to blunt ends. Cloning of PCR fragments. Treatment of PCR products containing 3'-A overhangs with T4 DNA Polymerase and dNTPs produces blunt ends, which greatly increases cloning efficiencies. Production of site-specific mutations. Because T4 DNA Polymerase does not displace oligonucleotides that are hybridized to DNA, it can be used for site-specific mutagenesis by primer extension of mutated oligonucleotides hybridized to single-stranded DNA templates. Labeling of 3'-termini of DNA molecules and synthesis of strandspecific probes using the exonuclease and polymerase activities of T4 DNA Polymerase.
T7 DNA Polymerase, Unmodified

T7 DNA Polymerase has both a template-directed 5' 3' DNA polymerase activity and a potent 3'5' exonuclease activity. The enzyme is a tightlybound complex of T7 phage-encoded gene 5 protein and E.coli hostencoded thioredoxin. T7 DNA Polymerase is highly processive; it synthesizes long stretches of DNA before dissociating from the template. The rate of elongation is also much faster than that of most other DNA polymerases. The unmodified form of T7 DNA Polymerase should not be confused with T7 DNA polymerase preparations from which exonuclease activity has been removed by genetic engineering or by oxidation during purification. Applications Primer extension of long DNA molecules. Conversion of 5'- and 3'-protruding ends to blunt ends. Labeling of 3'-ends of DNA. In situ detection of DNA fragmentation associated with apoptosis. PCR-203L PCR-203S PCR-104L PCR-104S
1000 U 200 U 250 rxns 50 rxns
PCR-204L PCR-204S PCR-205L PCR-202S PCR-202L
500 U 100 U 500 U 200 U 1000 U
! T : 040-27017477 / 65916167
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Real Time PCR SYBR Green & Probes based systems

FailSafe GREEN Real-Time PCR System
The FailSafe GREEN Real-Time PCR System extends the unsurpassed specificity, sensitivity, and consistency of the FailSafe PCR System to quantitative PCR applications. Like the FailSafe PCR System, this kit ensures successful quantitative PCR the first time and every time. The FailSafe Real-Time PCR System incorporates SYBR Green I dye for the detection of double-stranded DNA generated during PCR. During each phase of DNA synthesis, the SYBR Green I dye binds to the amplified PCR products and the amplicon quantity is monitored in real time by measuring its fluorescence. The incorporation of SYBR Green I dye into a realtime PCR reaction provides great flexibility because no target-specific probes are required. The system also incorporates an extensively tested set of 12 FailSafe PCR PreMixes, representing various PCR conditions. These PreMixes contain everything you need for a successful quantitative PCR: SYBR Green I dye, dNTPs, buffer, and varying amounts of MgCl2 and EPICENTREs patented FailSafe PCR Enhancer (with betaine). The system also includes ROX, a passive reference fluorescent dye, which may be required for signal normalization in SYBR Green dye reactions when using ABI real-time PCR instruments.
PCR
Real Time PCR
FailSafe PROBES Real-Time PCR System

The FailSafe PROBES Real-Time PCR System enables rapid, precise optimization of PCR experiments in which the product is detected using fluorescent target-specific labeled probes. The FailSafe PROBES RealTime PCR System uses the FailSafe PCR Enzyme Blend, which has been proven to amplify the most difficult templates with extremely high specificity, sensitivity, and fidelity, and a set of 8 specially chosen PCR 2X PreMixes representing a complete range of optimal real-time PCR conditions. Benefits Simple, one-step optimization protocol means real-time PCR reactions that work first time and every time, saving you time and money. Eight PCR Premixes contain all components for screening a complete range of real-time PCR conditions with your templates, primers and probes. Sensitivity, specificity, and PCR efficiency are extremely high due to the use of a unique enzyme blend and FailSafe PCR Enhancer technology. FailSafe PROBES Real-Time PCR System is compatible with all real-time PCR instruments and fluorescent probes. No hot-start is needed. Passive Reference Dye and Stabilizer are included. Product FailSafe PROBES Real-Time PCR Optimization Kit^ FailSafe PROBES Real-Time PCR PreMix-Choice Kit^^ FailSafe PROBES Real-Time PCR PreMix-Choice Kit^^ Qty 48 25 l Rxns 200- 25 l Rxns 5x200- 25 l Rxns
FIRST TIME Perform real-time PCR with your template & primers using the FailSafe GREEN Realtime PCR Optimization Kit & select the optimal Real-time PCR Premix.
And EVERY TIME For consistent PCR results, choose the PCR Premix identified as optimal when you get the FailSafe GREEN Real-time PCR Premix ChoiceKit
Cat. No. FSP51048 FSP51200
Benefits Real time PCR Premixes contain all components for quantitative PCR using Sybr Green I dye. No specific probes required. Includes ROX, an internal reference dye. Kits available for both tube and capillary instruments. Product FailSafe Green Real-Time PCR PreMix Selection Kit * FailSafe Green Real-Time PCR PreMix Selection Kit ** FailSafe Green Real-Time Capillary PCR PreMix Selection Kit # FailSafe Real-Time Capillary PCR System ## FailSafe Real-Time Capillary PCR System ## Qty 96- 25 l rxns 400- 25 l rxns
FSP5101K
^Contents: FailSafe PCR Enzyme Blend, 8 FailSafe PROBES Real-Time PCR 2X PreMixes, Passive Reference Dye, and Stabilizer. ^^Contents: FailSafe PCR Enzyme Blend, Passive Reference Dye, and stabilizer. FSP51200 includes your choice of any two FailSafe PROBES Real-Time PCR 2X PreMixes. FSP5101K includes your choice of any ten FailSafe PROBES Real-Time PCR 2X PreMixes.
Cat. No. FSR0360 FSR03200
TAQurate GREEN Real-Time PCR MasterMix TAQurate PROBES Real-Time PCR MasterMix
It uses a single 2X solution containing everything needed for Real-Time PCR. Just add one volume of a cocktail containing your template, primers, and probes(for the PROBES MasterMix) to one volume of the TAQurate Real-Time PCR 2X MasterMix and run the reaction in your real-time PCR instrument. Cat. No. Product TAQurate GREEN Real-Time PCR MasterMix*** TAQurate GREEN Real-Time PCR MasterMix*** TAQurate PROBES Real-Time PCR MasterMix## TAQurate PROBES Real-Time PCR MasterMix## TAQurate PROBES Real-Time PCR MasterMix## Qty 96 25-l rxns 400 25-l rxns 48 25-l rxns 200 25-l rxns 1000 25-l rxns
FSRC3832 FSRC3896 FSRC38384
32- 20 l rxns 96- 20 l rxns 4x96 20 l rxns
TM049096 TM046400 TP411048 TP411200 TP41101K
* Contents: FailSafe PCR Enzyme Mix, 12 FailSafe Real-Time PCR 2X PreMixes Passive Reference Dye. ** Contents: FailSafe PCR Enzyme Mix. Choice of two FailSafe Real-Time PCR 2X PreMixes (Choose any two when ordering; PreMixes are not available separately.), Passive Reference Dye. # Contents: FailSafe PCR Enzyme Mix, 8 FailSafe Real-Time PCR Capillary 2X Premixes. ## Contents: FailSafe PCR Enzyme Mix Choice of FailSafe Real-Time PCR Capillary 2X Premix(es) (PreMixes are not available separately.)
***Contents: TAQurate Real-Time PCR Enzyme Blend, TAQurate GREEN Real-Time 2X PCR MasterMix, Passive Reference Dye, and Stabilizer. ### Contents: TAQurate PROBES Real-Time PCR 2X MasterMix, TAQurate Real-Time PCR Enzyme Blend, Passive Reference Dye, and Stabilizer.
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dNTPs and Markers

dNTPs for PCR
High Quality: Cat. No. D08104 D32104 D59104 D0810A/C/G/T D03210A/C/G/T D05910A/C/G/T D1905U Product Premixed dNTP solutions (2.5 mM) Premixed dNTP solutions (6.25 mM) Premixed dNTP solutions (25 mM) 10 mM dNTP soln. of dATP/dCTP/dGTP/dTTP 25 mM dNTP soln. of dATP/dCTP/dGTP/dTTP 100 mM dNTP soln. of dATP/dCTP/dGTP/dTTP 20 mM dUTP soln. Qty 4 ml, 10 mol M12 1.6 ml, 10 mol 400 l, 10 mol 1ml of 10 mol M24 400 l of 10 mol 100 l of 10 mol 250 l of5 mol M-201 Full Range DNA Ladder Bundle 100 lanes each 100 bp+1.5 Kb DNA Ladder M15 M16 M23 1 Kb DNA Ladder 100 bp DNA Ladder 100 bp DNA Ladder 100 bp+1.5 Kb DNA Ladder
PCR
DNTPs / Ladders
Standard DNA Markers

Cat. No. M11 Product 1 Kb DNA Ladder Qty 50 g 250 g 50 g 250 g 50 g 250 g
Full Range DNA Ladder Bundle

Cat. No. Product Qty 500 l each
For routine applications: Cat. No. N001 N002 N003 N004 N005 N006 N007 N008 Product dATP, 100mM water solution dATP, 100mM water solution dCTP, 100mM water solution dCTP, 100mM water solution dGTP, 100mM water solution dGTP, 100mM water solution dTTP, 100mM water solution dTTP, 100mM water solution Qty 50 l 250 l 50 l 250 l 50 l 250 l 50 l 250 l
The 3 fragment mixtures yield ladders of the following range: 50-100bp linear scale : consists of 50,75,100, 150, 200, 300, 400, 500, 600, 800, 1000 bp 100-3000 bp linear scale : consists of 100, 150, 200, 300, 400, 500, 600, 800, 1000, 1500, 2000, 3000 bp 0.5-10kbp linear scale : consists of 0.5, 0.6, 0.8, 1, 1.5, 2, 3, 4, 5, 6, 8, 10kbp. Concentration: 100ng/ul
Low range DNA Ladder

Cat. No. M-202 Product Low range DNA Ladder 100 lanes Qty 500 l
50-100 bp linear scale The fragment mixture yields a ladder of the following ds fragments: 50,75,100,150,200,300,400,500,600,800,1000bp. Concentration: 100ng/ul
Standard DNA Markers

Cat. No. M01 M02 M03 M04 M05 M06 M17 M18 M07 Product Lambda DNA/HindIII Lambda DNA/HindIII Lambda DNA/Bme18I(AvaII) Lambda DNA/Bme18I(AvaII) Lambda DNA/BssT1I(StyI) Lambda DNA/BssT1I(StyI) Lambda DNA/BglI Lambda DNA/BglI pUC19/MspI Qty 100 g 500 g 100 g 500 g 100 g 500 g 100 g 500 g 50 g
Mid range DNA Ladder

Cat. No. M-203 Product Mid range DNA Ladder 100 lanes Qty 500 l
100-300 bp linear scale The fragment mixture yields a ladder of the following ds fragments: 100,150,200,300,400,500,600,800,1000, 1500, 2000, 3000 bp. Concentration: 100ng/ul
High range DNA Ladder

Cat. No. M-204 Product High range DNA Ladder 100 lanes Qty 500 l
0.5-10 kbp linear scale The fragment mixture yields a ladder of the following ds fragments: 0.5, 0.6, 0.8, 1, 1.5, 2, 3, 4, 5, 6, 8, 10 kbp. Concentration: 100ng/ul
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Ladders / PCR enhancer / Buffers

Log Scale DNA Ladders DNA Ladder Bundle 5
500-5000 bp log scale: 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000 bp 200-2000 bp log scale: 200, 400, 600, 800, 1000, 1200, 1400, 1600,1800, 2000 bp 50-500 bp log scale: 50,100,150,200,250,300,350,400,450,500 bp 100-1000 bp log scale: 100, 200, 300, 400, 500, 600, 700, 800, 900,1000 bp 1-10 kb log scale: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 kbp Concentration: 100ng/l Cat. No. M-211 Product DNA Ladder Bundle 00 lanes each Qty 500 l each
PCR
Ladders / PCR ENHANCER / Buffers
DNA Preparations
Cat. No. D10 D11 D02 D03 D04 D05 D06 Product DNA phage lambda (dam-, dcm-) DNA phage lambda DNA phage T7 DNA pBR322 DNA pBR322 DNA pUC19 DNA pUC19 Qty 500 g 500 g 500 g 50 g 250 g 50 g 250 g
MasterAmp 10X PCR Enhancer

EPICENTREs patented MasterAmp 10X PCR Enhancer (with betaine) substantially improves PCR performance and allows improved sensitivity and specificity for amplification of many DNA templates. Using MasterAmp PCR Enhancer in PCR eliminates the base-pair composition dependence of DNA melting, increases enzymes thermal stability, suppresses pauses of DNA polymerase, and reduces secondary DNA structure. As a result, long DNA templates; difficult DNA templates (e.g., those with a high GC content), and those with other complex structures can be amplified at higher denaturing temperatures, significantly reducing the effect of secondary structure without loss of enzyme activity. Cat. No. ME81201 ME81205 Product MasterAmp 10X PCR Enhancer MasterAmp 10X PCR Enhancer MasterAmp 10X PCR Enhancer 25 mM MgCl2 Qty 1.5 ml 5 ml 10 ml 25 ml
DNA Ladder Bundle 3

Cat. No. M-212 Product DNA Ladder Bundle 300 lanes each Qty 500 l each
50-500 bp log scale: 50,100,150,200,250,300,350,400,450,500 bp 200-2000 bp log scale: 200, 400, 600, 800, 1000, 1200, 1400, 1600,1800, 2000 bp 1-10 kb log scale: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 kbp Concentration: 100ng/l
Other Ladders
Cat. No. M-213 M-214 M-215 M-216 M-217 Product 50 bp ladder (50-500 bp log scale) 100 bp DNA Ladder (100-1000 bp log scale) 200 bp DNA Ladder (200-2000 bp log scale) 500 bp DNA Ladder (500-5000 bp log scale) 1 kbp DNA Ladder (1-10 kbp log scale Qty 500 l 500 l 500 l 500 l 500 l
ME81210 FB4250
Sterile Nuclease-Free Water

Sterile Nuclease-Free Water is useful for many molecular biology applications. Our water is free of DNA exonuclease and endonuclease activities, and RNase activity.
Enzyme Storage Buffer

Enzyme Storage Buffer, for dilution of enzymes supplied in this buffer, is liquid at -20C, but is frozen at -80C. Most highly-purified enzymes are stable under either of these conditions, but should not be stored at intermediate temperatures between about -30C to -40C because the storage buffer can freeze and thaw, potentially inactivating the enzyme. Some enzymes require storage buffers with cofactors or other components. Confirm that Enzyme Storage Buffer is suitable for the enzyme. Some enzymes are less stable at very dilute concentrations or you may see losses in activity due to binding of the enzyme to the walls of the tube. Enzyme Storage Buffer: 50% glycerol containing 50mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton X-100. Cat. No. Product 10X TA Buffer 10X TA Buffer Sterile Nuclease-Free Water Enzyme Storage Buffer Qty 1.5 ml 6.0 ml 50 ml 1 ml
Protein Molecular weight Marker

Mixture of 7 purified proteins supplied in gel loading buffer (50mM TrisHCl(pH 6.8), 100mM DTT, 2% SDS, 0.1% bromophenol Blue, 10% Glycerol) for direct loading. The proteins are: 116 kDa, 97.4 KDa, 66.2 Kda, 37.6 Kda, 28.5 Kda, 18.4 Kda, 14.0 Kda. Cat. No. PS-101 Product Protein MW Marker Qty 500 l
10X TA Buffer
TA Buffer is compatible with a wide variety of restriction endonucleases and nucleic acid modifying enzymes. Use of TA Buffer can reduce the number of buffer changes required during nucleic acid sample processing, saving time and eliminating sample loss that commonly occurs with buffer changes. Table 1 shows many enzymes that are active in TA Buffer.
TA6115 TA6160 W7350ML ESB4901
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Custom Primer / Oligo Synthesis

Yield Guarantee
Guaranteed A260 units for unmodified oligos. Scale ( mole) 0.05 0.10 0.20 1.00 Scale ( mole) 0.05 0.10 0.20 1.00 Desalted 5-10 8-15 12-20 60-80 HPLC 1.25-2.50 4-5 3-7.5 10-25 Cartridge 1.25-2.50 4-5 3-7.5 10-25 PAGE 1.25-2.50 4-5 3-7.5 10-25
PCR
Custom Primer / Oligo Synthesis & Modifications
Random Primers
Cat. No. EM-N017 EM-N018 EM-N019 EM-N046 EM-N047 EM-N020 Sequence (length) Random mix of all 4 nucleotides at each position (6) - (dN) 6 Random mix of all 4 nucleotides at each position (9) - (dN) 9 Random mix of all 4 nucleotides at each position (12) - (dN) 12 Random mix of all 4 nucleotides at each position (18) - (dN) 18 Random mix of all 4 nucleotides at each position (24) - (dN) 24 Random mix of all 4 nucleotides at each position (36) - (dN) 36 Qty 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U
Custom Primer / Oligo Modifications 5 or 3 Modification

Phosphate 5 Carboxyl 3 Carboxyl Fluorescein/6-FAM Primary Amine TAMRA 5 Cy5 / Cy3 3 Cy5 / Cy3 5 or 3 Cy5.5 / Cy2 Acridine Psoralen (C2 / C6) EDANS/DABCYL HEX TET ROX JOE Biotin Digoxigenin 2, 3 Dideoxy A/C/G/T 2, 4 Dinitrophenyl
Backbone modifications
Please enquire for Backbone Modified Oligonucleotides and 5 5 or 3 3 Linkages at different scale of synthesis
Oligo dT
Cat. No. EM-N048 EM-N049 EM-N050 EM-N051 EM-N052 EM-N053 EM-N054 EM-N055 EM-N056 EM-N057 EM-N058 Sequence (length) Oligo-dT (10) Oligo-dT (20) Oligo-dT (30) Oligo-dT (40) Oligo-dT (50) Oligo-dT (60) Oligo-dT (70) Oligo-dT (80) Oligo-dT (90) Oligo-dT (100) Mixture of oligo-dT(10) through oligo-dT (100) Qty 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD 260 U 1 OD260 U
Other DNA oligo modifications available.

Please enquire.
Universal Primers
Cat. No. PM-201 PM-202 PM-203 Sequence (length) T3-Promoter, 17-mer, 10 M T7-Promoter, 21-mer, 10 M T7-Terminator, 19-mer, 10 M SP6-Promoter, 18-mer, 10 M SP6-Promoter, 24-mer, 10 M M13/pUC forward, 17-mer (-20), 10 M M13/pUC reverse, 19-mer (-24), 10 M M13/pUC forward, 24-mer (-47), 10 M M13/pUC reverse, 24-mer (-46), 10 M Qty 100 l (1 nmol) 100 l (1 nmol) 100 l (1 nmol) 100 l (1 nmol) 100 l (1 nmol) 100 l (1 nmol) 100 l (1 nmol) 100 l (1 nmol) 100 l (1 nmol)
Universal Primers
Cat. No. P5710F P5710R P5711F P5711R P5706S P5707T P5703T Sequence (length) Lambda gt10 Primer, forward (24-mer) Lambda gt10 Primer, Reverse (24-mer) Lambda gt11 Primer, forward (24-mer) Lambda gt11 Primer, Reverse (24-mer) SP6 Pramoter Primer (24-mer) T7 Pramoter Primer (24-mer) T3 Pramoter Primer (23-mer) Qty 2 g 2 g 2 g 2 g 2 g 2 g 2 g
PM-204 PM-205 PM-206 PM-207 PM-208 PM-209
ddNTPs for Sequencing

Please inquire for details
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cDNA Synthesis and RT-PCR

MonsterScript 1st-Strand cDNA Synthesis Kit
The MonsterScript 1st-Strand cDNA Synthesis Kit is optimized for generating full-length first-strand cDNA from multiple mRNA species in samples containing total cellular or purified polyadenylated RNA. The cDNA generated is useful for a variety of subsequent applications, including use as a template for PCR, real-time PCR, or other amplification methods. MonsterScript 1st-Strand cDNA Synthesis Kit uses MonsterScript Reverse Transcriptase, a novel proprietary reverse transcriptase that lacks RNase H activity. The enzymes lack of RNase H activity contributes to its ability to make longer cDNAs and more complete libraries of firststrand cDNA molecules. Benefits MonsterScript Reverse Transcriptase lacks RNase H, enabling improved synthesis of full-length cDNA even for long mRNA. MonsterScript is thermostable, permitting reverse transcription at temperatures >50C, which reduces RNA secondary structure and improves priming specificity. MonsterScript RT PreMix contains optimized concentrations of dNTPS, Mg +2 and Betaine for superior performance and minimal pipetting steps. Betaine in the cDNA Synthesis PreMix reduces pausing and stops during reverse transcription. The kit includes both an oligo(dT)-containing and a random nonamer primer. First-strand cDNA can be made from picogram amounts of total RNA. The cDNA is a good template for subsequent end-point PCR or real-time PCR. A potent RNase Inhibitor is included. Cat. No. MS040910 MS041050 Product MonsterScript 1st-Strand cDNA Synthesis Kit MonsterScript 1st-Strand cDNA Synthesis Kit Qty 10 Rxns. 50 Rxns.
RT-PCR
Reverse Transcriptases
MMLV Reverse Transcriptase

MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is an RNA- and DNA-dependent DNA polymerase requiring an oligodeoxyribonucleotide primer for initiation of elongation.It is useful for first strand cDNA synthesis in constructing cDNA libraries, as well as for production of templates for RT-PCR amplifications. MMLV Reverse Transcriptase (MMLV RT) lacks a 3' 5' exonucleolytic proofreading function and has a weak RNase H activity. Cat. No. M6125H M6110K M4425H M4410K Product MMLV Reverse Transcriptase (10 U/l) Qty 2500 U 10000 U 2500 U 10000 U
MMLV Reverse Transcriptase (10 U/l) MMLV Reverse Transcriptase (50 U/l) MMLV Reverse Transcriptase (50 U/l)
Includes 10X Reaction Buffer and DTT.
MasterAmp Tth DNA Polymerase

Also has Reverse Transcriptase activity. For details please see page#.
MasterAmp High Fidelity RT-PCR Kit

The MasterAmp High Fidelity RT-PCR Kit is ideal for synthesis of accurate double-stranded cDNA products that will be used for cloning, sequencing, expression, or transcription analysis. The kit uses MMLV-RT Plus, a reverse transcriptase with an unsurpassed accuracy and reliability, and MasterAmp TAQurate DNA Polymerase, a high fidelity PCR enzyme mix. The kit also contains an optimized 2X RT-PCR Buffer PreMix (containing dNTPs, MgCl2, and buffer), random and oligo(dT) primers, and the MasterAmp PCR Enhancer with betaine. Applications
MonsterScript Reverse Transcriptase

EPICENTREs MonsterScript Reverse Transcriptase is a magnesium-dependent thermostable reverse transcriptase that completely lacks RNase H activity. The enzyme is highly efficient at producing full-length cDNA from RNA templates up to or greater than 15 kb. Benefits Thermostable reverse transcriptase. Full activity up to 65C enables reverse transcription through regions of high GC content or difficult secondary structure, and increased specificity when using gene-specific primers. Completely lacks RNase H activity (RNase H-Minus) for fulllength cDNA synthesis from RNA up to or greater than 15 kb. Greater sensitivity in RT-PCR. MonsterScript Reverse Transcriptase very efficiently produces cDNA from picogram quantities of total RNA. In addition, the MonsterScript Reaction Buffer is compatible with PCR reactions so more of the RT reaction can be used in the PCR reaction. Cat. No. MSTA5110 Product MonsterScript Reverse Transcriptase MonsterScript Reverse Transcriptase Qty 10 Rxns.
Highly accurate RT-PCR amplification of RNA templates for Cloning Sequencing Expression Transcription analysis All purpose RT-PCR in which full-length amplification and high accuracy are essential. Multiplex RT-PCR.
ONE-STEP RT-PCR The one-step procedure performs first-strand cDNA synthesis and PCR in one tube using RNA specific primers. STANDARD TWO-STEP RT-PCR In the two-step procedure, first-strand synthesis is performed using oligo(dT) or random primers. A small amount of cDNA is then used in subsequent PCR. Cat. No. RF91025 RF910100 Product MasterAmp High Fidelity RT-PCR Kit MasterAmp High Fidelity RT-PCR Kit Qty 25 rxns 100 rxns
MSTA5124
24 Rxns.
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cDNA Synthesis and RT-PCR

MasterAmp RT-PCR Kit for High Sensitivity
The MasterAmp RT-PCR Kit for High Sensitivity is a single-enzyme, single-tube RT-PCR system. It is capable of amplifying RNA from a single gene in as little as 1 pg of total cellular placental RNA (e.g., human chorionic gonadotropin). It is ideal for amplifying low-copy-number RNA transcripts. The MasterAmp RT-PCR Kit for High Sensitivity uses RetroAmp RT DNA Polymerase, which is both thermostable and has reverse transcription activity. This kit is able to synthesize cDNA at higher temperatures (60C). The higher temperature RT can significantly reduce RNA secondary structure, thus increasing both specificity and sensitivity of RT reactions. The high sensitivity of this kit is further augmented by combining both RT and PCR into one single step in which all cDNA synthesized during RT is used as template for PCR amplification. One-Tube One-Step One-Enzyme RT-PCR for Gene Specific Primers Perform first-strand cDNA synthesis and PCR uninterrupted, in one tube using specific primers. One-Tube Two-Step RT-PCR for Non-Specific Primers Perform first-strand cDNA synthesis using random oligo dT primers. In the subsequent PCR reaction, use cDNA generated and specific primers . Benefits High sensitivity RT-PCR from as little as 1 pg of total cellular RNA. Out-performs similar products from other vendors. Reduces effect of RNA secondary structure by performing RT reactions at higher temperature. Reduces chance for cross contamination by using one enzyme in a one-step and one-tube reaction protocol with no sample transfer. MasterAmp PCR Enhancer Technology enhances PCR performance with GC-rich and other difficult templates. Product MasterAmp RT-PCR Kit for High Sensitivity MasterAmp RT-PCR Kit for High Sensitivity Qty 25 rxns 100 rxns Cat. No. MAR03825 MAR03100 Product
RT-PCR
Kits for RT-PCR/Realtime RT-PCR
MasterAmp GREEN Real-Time RT-PCR Kit

The MasterAmp GREEN Real-Time RT-PCR Kit provides all the necessary components to perform high-sensitivity one-step quantitative RT-PCR using SYBR Green I Dye for detection. The kit uses RetroAmp RT DNA Polymerase, a thermostable enzyme that has efficient reverse transcriptase (RT) activity up to 70C and lacks RNase H activity. Hightemperature reverse transcription reduces the secondary structure of RNA templates and results in significantly increased specificity and sensitivity. Extremely high sensitivity and specificity. Easy-to-use PreMix contains all components for successful quantitative RT-PCR, including SYBR Green I dye. A thermostable enzyme increases sensitivity and specificity by reducing RNA secondary structure. MasterAmp PCR Enhancer increases sensitivity and specificity by reducing polymerase pausing and stops during reverse transcription and PCR. SYBR Green I dye for detection saves time and expense compared to use of labeled primers. The kit uses flexible protocols for real-time detection on almost all real-time PCR thermocyclers.
Qty 25 rxns 100 rxns
MasterAmp GREEN Real Time RT-PCR Kit MasterAmp GREEN Real Time RT-PCR Kit
Contents: RetroAmp RT DNA Polymerase, 2X Green RT-PCR PreMix, MasterAmp 10X PCR Enhancer, 25 mM MgCl 2, 25 mM MnSO 4, Sterile Water
Cat. No. RT71225 RT712100
HIV Reverse Transcriptase (Jena Bioscience)

HIV-1 Reverse Transcriptase (RT) is a heterodimer consisting of phageencoded p66 and p51 subunits. HIV-1 RT is used by the Human Immunodeficiency Virus-1 to transcribe its RNA genome into single-stranded DNA. HIV-1 RT can be used for incorporation of nucleotide analogs into DNA by RTPCR or as a standard for assaying HIV-1 RT activity in human serum. Although the p66 subunit is responsible for most of the polymerase activity as well as RNase H activity, the 1:1 complex with p51 subunit shows increased processivity and activity. The p66 subunit is responsible for polymerase activity as well as RNase H activity and shows this activity also in the absence of the p51 subunit. In the absence of p51, the p66 subunit forms homodimers. HIV-2 reverse transcriptase (RT) is used by the Human Immunodeficiency Virus-1 to transcribe its RNA genome into single-stranded DNA. HIV-2 RT can be used for incorporation of nucleotide analogs into DNA by RT-PCR or as a standard for assaying HIV-2 RT activity in human serum. In contrast to HIV-1 RT, HIV-2 RT is not inhibited by nonnucleoside RT inhibitors (NNRTIs). Cat. No. PR-351 Product HIV-1 RT(Human Immunodeficiency Virus1 Reverse Transcriptase) Recombinant, E. coli HIV-1 RT, p51 subunit (Human Immunodeficiency Virus1 Reverse Transcriptase) Recombinant, E. coli HIV-1 RT, p66 subunit(Human Immunodeficiency Virus1 Reverse Transcriptase) Recombinant, E. coli Qty 10 g
Contents: RetroAmp RT DNA Polymerase, 20X RT-PCR Buffer, MasterAmp 10X PCR Enhancer, 25 mM MgCl 2, 25 mM MnSO 4, dNTP Mix, 2.5 mM each, Control Template and Primer Mix, Sterile Water
Reverse Transcriptase (Sibenzyme)

Cat. No. E317 E318 Product M-MulV Reverse Transcriptase M-MulV Reverse Transcriptase Qty 1000 U 5000 U
PR-353
10 g
PR-354
10 g
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cDNA, Gene, and PCR Cloning

CopyControl cDNA, Gene & PCR Cloning Kits
Up to ~25% more cDNA clones can be obtained in a library if the cDNA clones are present in the host cell at only about one copy per cell (personal communication from P. Carninci and W. Szybalski labs), presumably due to reduced detrimental effects on host cell metabolism and lower toxicity of expressed clone sequences and gene products. CopyControl cDNA, Gene & PCR Cloning Kits provide a rapid and improved method (Figure 1) for cloning any blunt-end DNA fragments, such as blunt-end genes, PCR products, or cDNAs, at single-copy, with the capability to induce to high-copies at will. The CopyControl pCC1 (Blunt) Vector provided in the kits contains both the E. coli F-factor single-copy origin of replication ( ori ) and an inducible high-copy ori V. Replication does not occur using the oriV unless and until the trfA gene in the chromosome of TransforMax EPI300 cells, which is under the control of a tightly-regulated promoter, is induced. Thus, CopyControl cDNA, PCR, or gene clones can be grown at single copy to ensure insert stability and successful cloning of sequences that are toxic to the host cell. Then, any desired clone can be induced at will to up to 200 copies per cell for high yields of DNA for all downstream applications. Cat. No. CCECPCR1 Product CopyControl cDNA, Gene & PCR Cloning Kit * (with Electrocompetent cells) CopyControl cDNA, Gene & PCR Cloning Kit * (with Chemically competent cells) CopyControl Induction Solution
CLONING
PCR Cloning
Qty 20 Rxns
CCPCR1CC
20 Rxns
CCIS125
25 ml
* Contents: CopyControl pCC1 (Blunt-Cloning Ready) Vector, PCR Precipitation Solution, 10X Reaction Buffer, End-Repair Enzyme Mix, Fast-Link DNA Ligase, EpiLyse Solution, EpiBlue Solution, TransforMax EPI300 Competent E. coli, CopyControl Induction Solution, Control PCR Product, Supercoiled DNA Size Marker, Water
PlasmidMAX DNA Isolation Kit

PlasmidMAX DNA Isolation Kit is designed to purify plasmids free from RNA and genomic DNA. The resulting DNA is high quality, permitting average sequence reads of greater than 600 bases for plasmid templates between 3 and 40 kb. This simple procedure requires only two 1.5-ml tubes, thus avoiding excess plastic waste. The purification method is scalable to larger volumes, so separate kits are not needed for varying sample sizes.
Cat. No. PMX51050
Product PlasmidMAX DNA Isolation Kit Minipreps
Qty 50
Figure 1. Efficient, blunt-end cloning of any cDNA, PCR product or other dsDNA fragment up to 15 kb and screening of clones can be completed in 24 hours using the CopyControl cDNA, Gene & PCR Cloning Kit. Clones can be kept at one copy per cell for clone stability and then, whenever desired, any clone can be induced to high-copy-number (up to 200 copies per cell) for high yields of DNA. Size of the PCR Product cfu/g of PCR Cloned Product 1.4 kb 5 kb 10 kb 15 kb >1 X 10 7 1 X 10 7 3.5 X 10 6 9.2 X 10 5 Percent Full-Length Clones >90% 90% 80% 30%
Plasmid-Safe ATP-Dependent DNase

Plasmid-Safe ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations. Such preparations are frequently contaminated with fragments of bacterial genomic DNA generated during alkaline lysis. Other purification options, such as spin-columns or even CsCl centrifugation, do not effectively remove these contaminants and require further purification steps. Contaminating DNA fragments left behind by these methods ultimately can become ligated into your cloning vector, resulting in false positives and high backgrounds, or erroneous sequence data. Plasmid-Safe ATP-Dependent DNase digests linear double-stranded DNA to deoxynucleotides at slightly alkaline pH and, with lower efficiency, closed-circular and linear single-stranded DNA. The enzyme has no activity on nicked or closed-circular double-stranded DNA or supercoiled DNA. Cat. No. E3101K E3105K E3110K R109AT Product Plasmid-Safe ATP-Dependent Dnase** (10 U/l) Plasmid-Safe ATP-Dependent Dnase** (10 U/ l) Plasmid-Safe ATP-Dependent Dnase** (10 U/ l) ATP Solution (500 l of a 10 mM solution (pH 7.0)) Qty 1000 U 5000 U 10000 U 5 moles
Table 1. PCR Product Cloning Results Benefits Get more cDNA or PCR clones and more complete clone libraries up to 15 kb, including those that may be unstable or encode proteins that are toxic to the E. coli host cells (Table 1). Clones having mutations in genes that are toxic to the host cell are selectively favored at high-copy. Keep clones or a clone library at one copy per cell for insert stability. Then, induce up to 200 copies per cell at will Screen the size of the clones in 1 hour or less without the need for overnight cultures or restriction endonuclease digests.
**Includes Plasmid-Safe 10X Reaction Buffer and 25 mM ATP Solution.
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cDNA, Gene, and PCR Cloning

End-It DNA End-Repair Kit
The End-It DNA End-Repair Kit is used to convert DNA containing damaged or incompatible 5'- and/or 3'-protruding ends that result from shearing or restriction endonuclease digestion to 5'-phosphorylated, blunt-ended DNA for fast and efficient blunt-end cloning into plasmid, cosmid, fosmid, BAC, or other vectors. The conversion to blunt-ended DNA is accomplished by exploiting the 5' 3' polymerase and 3'5' exonuclease activities of T4 DNA Polymerase. T4 Polynucleotide Kinase and ATP are also included in the kit for phosphorylation of the 5'-ends of the blunt-ended DNA for subsequent ligation into a cloning vector. Benefits The repaired DNA is blunt-ended and 5'-phosphorylated for immediate blunt-end ligation into plasmid, cosmid, fosmid, or BAC vectors. High specific activity T4 DNA Polymerase and T4 Polynucleotide Kinase are included for complete conversion of 5'- or 3'overhang DNA to 5'-phosphorylated, blunt-ended DNA. Cat. No. ER0720 Product End-It DNA End-Repair Kit Qty 20 rxns
CLONING
PCR Cloning
DNA Fragment 2X Precipitation Solution

The DNA Fragment 2X Precipitation Solution enables rapid purification of double-stranded DNA fragments = 200 bp, including, for example, PCR reaction products, DNA fragments obtained in restriction enzyme digests, or double-stranded cDNA. If the precipitated DNA fragments are 5-phosphorylated and have ends that are compatible with the vector, they can be used for cloning without further purification Cat. No. DFP51002 DFP51012 Product DNA Fragment 2X Precipitation Solution DNA Fragment 2X Precipitation Solution Qty. 2.5 ml 12.5 ml
Transformation and Storage Solution (TSS)

Transformation and Storage Solution (TSS), developed at the U.S. National Institutes of Health, enables researchers to prepare competent E. coli in a single step and to transform the cells without heat-shock. TSS has been reported to be faster and easier than other methods of producing competent cells, such as the traditional CaCl 2 method described by Sambrook et al. or other high competency protocols. Transformation efficiencies depend on the strain of E. coli, as well as the nature and quality of the transforming DNA. Transformation efficiencies of 10 6-10 8 transformants/g DNA are typical. For example, the transformation efficiencies observed for E. coli strains DH1, DH5a, HB101, JM109, LE392, MM294, SCS-1, and XL1-Blue ranged from 1.5 x 10 7 to 1.0 x 10 8 per g of DNA. Single-step preparation of competent cells.
Contents: End-Repair Enzyme Mix including T4 DNA Polymerase and T4 Polynucleotide Kinase, End-Repair 10X Buffer, dNTP Solution, ATP
GELase Agarose Gel-Digesting Preparation

GELase Agarose Gel-Digesting Preparation contains a unique -agarose digesting enzyme developed at EPICENTRE for simple, quantitative recovery of intact DNA and RNA from low melting point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. The gel may be digested directly in the TAE, TBE, MOPS, and phosphate electrophoresis buffers or GELase Buffer may be added to or exchanged with those buffers for higher activity. Benefits Gentle procedurepurify even multi-megabase DNA or RNA that is intact and biologically active. Simple, flexible protocolsminimal hands-on time. Equilibration of the gel slice in GELase Buffer is not necessary, but can be used for higher activity or greater speed Fasta typical 200 mg gel slice in TAE buffer can be digested inless than 10 minutes using only 3 units of GELase Preparation Cost effectiveGELase is priced well below spin column or other gel-digesting methods. Cat. No. G09050 G09100 G09200 G31050 G31100 G31200 G191ML G195ML G1005ML G1025ML Product GELase (1 U/l*) GELase (1 U/l*) GELase (1 U/l*) GELase (0.2 U/l*) GELase (0.2 U/l*) GELase (0.2 U/l*) GELase 50X Reaction Buffer GELase 50X Reaction Buffer Ammonium Acetate Solution 5 M Ammonium Acetate Solution 5 M Qty 50 U 100 U 200 U 50 U 100 U 200 U 1 ml 5 ml 5 ml 5 x 5 ml
Store prepared cells at 70C with little or no loss in transformation efficiency. Transform cells without heat-shock. Transformation efficiencies of 10 6-10 8 transformants/g DNA are typically obtained
Cat. No. C905ML
Product Transformation & Storage Solution (TSS)
Qty 5 ml
TypeOne Restriction Inhibitor

DNA transformation can be difficult to achieve in many bacterial strains due to the presence of one or more restriction and modification (R-M) systems that cleave unmodified DNA that is recognized as foreign. TypeOne Restriction Inhibitor provides a powerful method for increasing transformation efficiencies in bacterial strains with type I R-M systems. Developed as a unique preparation of a phage protein called ocr,TypeOne Inhibitor is readily electroporated into cells along with transforming DNA. In vivo the protein acts as a molecular decoy that blocks the DNA binding site of type I R-M enzymes and inhibits DNA cleavage. Cat. No. TY0261H Product TypeOne Restriction Inhibitor Qty 100 g
Competent Cells
Please view page # 23
*Includes GELase 50X Reaction Buffer. Unit Definition: One unit of GELase Preparation digests 600 mg (~600 l) of molten 1% LMP-agarose gel in GELase Buffer in 1 hour at 45C.
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GENOMIC CLONING Fosmid Library Production

CopyControl Fosmid Library Production Kit CopyControl HTP Fosmid Library Production Kit
The CopyControl Fosmid Library Production Kits provide an efficient and improved method for constructing a library of cosmid-sized (approximately 40 kb) clones. The CopyControl Fosmid Library Production Kit contains the original pCC1FOS Vector, while the recently introduced CopyControl HTP Fosmid Library Production Kit includes a modified pCC1FOS Vector, called pCC2FOS (Figure 1). Both of these CopyControl Vectors contain both the E. coli F-factor single-copy origin of replication and the inducible high-copy oriV . CopyControl Fosmid clones are grown at single copy to ensure insert stability and successful cloning of encoded and expressed toxic protein. The CopyControl Fosmid clones can then be induced up to 50 copies per cell. The pCC2FOS Vector contains a primer cassette that optimizes endsequencing results, especially in a high-throughput setting. This primer cassette, engineered in conjunction with Agencourt Bioscience Corporation, eliminates wasteful vector-derived sequencing reads by having the 3' terminus of the forward and reverse primers anneal 3 nucleotides from the cloning site. In addition, the 7-base sequence at the 3'-end of each primer was specifically designed to minimize mispriming to any contaminating E. coli DNA present after template purification (Figure 2). TransforMax EPI300 E. coli , required for induction of the CopyControl Fosmid clones up to 50 copies per cell, are included with the CopyControl Fosmid Library Production Kits.
CLONING
CopyControl Cloning Systems
Figure 3. Overview of the process for preparing a fosmid library using CopyControl Fosmid Library Production Kits. Once the library has been prepared, individual clones can be cultured in small volume and induced to multiple-copy number for high yields of high purity DNA for fingerprinting, sequencing, etc. Benefits Make complete and unbiased CopyControl Fosmid libraries. CopyControl pCC1FOS and pCC2FOS Vectors are supplied Cloning-Readylinearized, dephosphorylated, purified, and ready for ligation. Maximize high-throughput end-sequence results using the pCC2FOS Vector. CopyControl Fosmid clones can be induced from single copy up to 50 copies per cell. Fosmid cloning utilizes high efficiency lambda packaging that eliminates background and false positives. No need for partial restriction endonuclease digests or pulse-field gel electrophoresis (PFGE) to prepare the genomic DNA for cloning. Cat. No. CCFOS110 F5FP010 F5RP011 CCIS125 CCFOS059 Product CopyControl Fosmid Library Production Kit* pCC1FOS/pEpiFOS Forward End-Sequencing Primer (50 mM) pCC1FOS/pEpiFOS Reverse End-Sequencing Primer (50 mM) CopyControl Induction Solution CopyControl HTP Fosmid Library Production Kit** pCC2 Forward Sequencing Primer (50 M) pCC2 Reverse Sequencing Primer (50 M) Qty 1 Kit 1 nmole 1 nmole 25 ml 1 Kit 1 nmole 1 nmole
Figure 1. CopyControl Vector map. The CopyControl pCC1FOS and pCC2FOS Vectors for CopyControl Fosmid library production are supplied linearized at the Eco72 I (blunt) site and then dephosphorylated and is ready for cloning end-repaired (blunt end) genomic DNA of approximately 40 kb.
HTFP061 HTRP062
Kit is sufficient for producing up to 10 complete and unbiased fosmid libraries. * Contents : CopyControl pCC1FOS Vector, End-Repair Enzyme Mixl, End-Repair 10X Buffer, dNTP Mix, Fast-Link DNA Ligase Fast-Link 10X Ligation Buffer, ATP Solution, GELase Gel-Digesting Preparation, GELase 50X Reaction Buffer, MaxPlax Lambda Packaging Extracts, Control DNA, Ligated Lambda Control DNA, EPI300 Plating Strain, Control Lambda Plating Strain CopyControl Induction Solution ** Same as kit contents above except that it contains CopyControl pCC2FOS Vector instead of pCC1FOS.
Figure 2. The CopyControl pCC2FOS Vector differs from the pCC1FOS Vector by the insertion of a new primer cassette that eliminates wasteful vector-derived sequencing reads and minimizes the potential for priming on the E. coli genome.
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Fosmid and BAC Library Production

EpiFOS Fosmid Library Production Kit
The EpiFOS Fosmid Library Production Kit provides all reagents needed to construct up to 10 complete and unbiased primary fosmid libraries using a novel cloning strategy (Figure 1). Genomic DNA is first sheared by passing it through a syringe needle. The sheared DNA is end-repaired to generate 5-phosphorylated blunt ends and size-selected using a low melting point agarose gel. Finally, the sizeselected DNA is ligated into the supplied linearized and dephosphorylated pEpiFOS-5 Fosmid Vector (Figure 2), packaged using ultra-high efficiency MaxPlax Lambda Packaging Extracts (>10 9 pfu/g for phage lambda), and plated on phage T1-resistant EPI100-T1R E. coli plating cells, all included in the kit. Fosmid pEpiFOS-5 has an F-factor origin, for replication in E. coli at single-copy and cos sites that enable packaging of cosmid-sized clones (35-45 kb) in E. coli phage lambda packaging extracts. The end result is a complete, unbiased and stable primary fosmid library. Note: Fosmid clones constructed in the pEpiFOS-5 Vector cannot be induced to high-copy number.
CLONING
CopyControl Cloning Systems
CopyControl BAC Cloning Kits(BamH I,EcoR I,Hind III)

CopyControl pCC1BAC Cloning-Ready Vectors
CopyControl BAC Cloning Kits are complete kits containing all molecular biology reagents you need for constructing high-quality, large-insert BAC (Bacterial Artificial Chromosome) libraries. Each kit has enough reagents to make a complete human genome-size library at 10X coverage. The kits feature the CopyControl pCC1BAC Vector which contains the E. coli F-factor single-copy origin of replication and an inducible high-copy oriV origin of replication. CopyControl BAC clones are grown at single-copy number to ensure insert stability. Then, clones can be induced to 10-20 copies per cell within 2 hours of adding CopyControl Induction Solution to the culture, for higher yields and higher purity DNA. CopyControl BAC Cloning Kits reduce the time and labor required to construct a BAC library. TransforMax EPI300 Electrocompetent E. coli , or Phage T1-resistant TransforMax EPI300-T1 R Electrocompetent E. coli , required for induction of CopyControl BAC clones to 10-20 copies per cell, are available separately. The CopyControl pCC1BAC Vector is also sold separately. The vector has been linearized at a unique restriction enzyme recognition site ( Bam H I, Hin d III, or Eco R I), dephosporylated, and highly purified to ensure very low background.
Figure 1. Production of a fosmid library using the EpiFOS Fosmid Library Production Kit.
Fig. 1. The CopyControl pCC1BAC Vectors are supplied linearized at either the unique BamH I-, Hin d III-, or Eco R I-site, dephosphorylated. pCC1BAC is derived from pBeloBAC11 and pIndigoBAC-5. Cat. No. CCBAC1B Figure 2. EpiFOS-5 Fosmid Vector. Cat. No. FOS0901 F5FP010 F5RP011 Product EpiFOS Fosmid Library Production Kit* pEpiFOS-5 Forward Sequencing Primer pEpiFOS-5 Reverse Sequencing Primer Qty 1 Kit 1 nmole 1 nmole CCBAC1E CCBAC1H CCIS125 CBAC311B CBAC311E Product CopyControl BAC Cloning Kit (BamH I) CopyControl BAC Cloning Kit (Eco RI) CopyControl BAC Cloning Kit (Hind III) CopyControl Induction Solution CopyControl pCC1BAC (BamH I Cloning-Ready) Vector CopyControl pCC1BAC (EcoR I Cloning-Ready) Vector CopyControl pCC1BAC (Hind III Cloning-Ready) Vector pCC1BAC/pIndigoBAC-5 Forward End-Sequencing Primer pCC1BAC/pIndigoBAC-5 Reverse End-Sequencing Primer Qty 1 Kit 1 Kit 1 Kit 25 ml 375 ng 375 ng
1 kit is sufficient for producing up to 10 complete and unbiased fosmid libraries. *Contents : pEpiFOS-5 Fosmid Vector*, End-Repair Enzyme Mix, End-Repair 10X Buffer, dNTP Mix, Fast-Link DNA Ligase, Fast-Link 10X, Ligation Buffer, ATP Solution, GELase Gel-Digesting Preparation, GELase 50X Reaction Buffer, MaxPlax Lambda Packaging Extracts, Ligated Lambda Control DNA, Control DNA, EPI100 Plating Strain, Control Lambda Plating Strain
CBAC311H BFP0701 BRP0801
375 ng 1 nmole 1 nmole
Kits sufficient for constructing the equivalent of one 10X human BAC lib.
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BAC Cloning- Vectors, Ladders, Purification of Clones

pIndigoBAC-5 (BamH I Cloning-Ready) Vector pIndigoBAC-5 (Hind III Cloning-Ready) Vector
pIndigoBAC-5 (Figure 1) is derived from pBeloBAC11 and pIndigoBAC and will accommodate and stably maintain DNA inserts of >100 kb. pIndigoBAC-5 has been linearized at its unique Bam H I or Hin d III site, dephosphorylated, and highly purified and is ready for cloning Bam H Ior Hin d III-cut genomic DNA. The linearized and dephosphorylated vectors are tested to ensure the completeness of linearization, dephosphorylation, and the integrity of the BamH I and Hin d III ends. Once DNA has been ligated into the pIndigoBAC-5 vector, BAC libraries can be produced using the high efficiency TransforMax EC100 Electrocompetent E. coli or the Phage T1-resistant TransforMax EC100T1 R Electrocompetent E. coli. These cells accommodate and stably maintain large BAC clones. Sequencing primers for BAC end-sequencing of pIndigoBAC-5 clones are available separately. Note: pIndigoBAC-5 clones cannot be induced to high-copy number.
CLONING
BAC Cloning
Converting Single-Copy BAC or Fosmid Clones to Multi-Copy-Inducible EZ-Tn5 <oriV/KAN-2> Insertion Kit
BAC-Tracker Supercoiled DNA Ladder

The BAC-Tracker Supercoiled DNA Ladder is suitable for estimating the size of large supercoiled DNAs, such as BAC (Bacterial Artificial Chromosome) clones, by agarose gel electrophoresis. The improved BAC-Tracker Ladder contains 7 (rather than 4) discreet supercoiled DNAs ranging from 8 to 165 kb (Figure 1). The additional 28 and 165 kb bands improve size estimations and the 8 kb band facilitates screening for empty or non-recombinant BAC clones. Supercoiled DNAs of 8, 28, 38, 55, 95, 120, and 165 kb.
Figure 1. Cloning-Ready pIndigoBAC-5 Vectors are supplied linearized at either the unique BamH I or Hind III site, completely dephosphorylated, and highly purified.
Figure 1. Supercoiled BAC DNA is sized by comparison with the BAC-Tracker Supercoiled DNA Ladder . Colonies from 3 different BAC clones (Lanes 1-3) were processed using the Colony Fast-Screen Kit (Size Screen) and analyzed by agarose gel electrophoresis. M, Improved BAC-Tracker Supercoiled DNA Ladder.
Cat. No. BACB085H BACH095H BFP0701 BRP0801
Product plndigoBAC-5 (BamH I-Cloning Ready) plndigoBAC-5 (Hind III-Cloning Ready) plndigoBAC-5 Forward Sequencing Primer plndigoBAC-5 Reverse Sequencing Primer
Qty 500 ng 500 ng 1 nmole 1 nmole
BAC/Fosmid Clone Purification BACMAX DNA Purification Kit

FosmidMAX DNA Purification Kit

Vector are supplied at 25 ng/l; vector is linearized at BamH I or Eco RI site and dephosphorylated. Primer is supplied at 50 M in TE Buffer.
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Cosmid Cloning / Lambda Packaging Extracts

pWEB-TNC Cosmid Cloning Kit pWEB Cosmid Cloning Kit
The pWEB::TNC and the pWEB Cosmid Cloning Kits facilitate rapid and efficient construction of unbiased cosmid libraries. The kits utilize a novel strategy of cloning end-repaired, randomly sheared DNA instead of the conventional approach of cloning fragments generated by partial restriction endonuclease digestion. First, genomic DNA is sheared by passing it through a syringe needle (not supplied with either kit). The sheared DNA is end-repaired to generate blunt ends and size-selected using a low melting point agarose gel. The size-selected DNA is then ligated into the supplied linearized and dephosphorylated pWEB::TNC or pWEB Cosmid Vector, packaged using ultra-high efficiency MaxPlax Lambda Packaging Extracts (>10 9 pfu/g for phage lambda), also included in the kit, and plated on the supplied EPI100 E. coli plating cells. The result is a complete and unbiased primary cosmid library.
CLONING
Cosmids & Phage extracts
MaxPlax Lambda Packaging Extracts

MaxPlax Lambda Packaging Extracts offer maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E.coli strains, BHB2690 and BHB2688, as described by Hohn.MaxPlax Extracts utilize a restrictionfree E. coli K-12 strain, NM759, in place of strain BHB2690. The sonicated extracts of NM759 are combined with extracts of BHB2688 to produce MaxPlax Extracts. Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging efficiency of greater than 1 x 10 9 pfu/g of Control Lambda DNA for one year from the date of purchase if stored properly. A Certificate of Analysis stating actual packaging efficiency (pfu/g DNA) for each respective lot of MaxPlax Extracts is provided. Benefits
pWEB::TNC Cosmid Cloning Kit

The pWEB::TNC Cosmid Cloning Kit contains the pWEB::TNC Cosmid Vector. This vector can be used in all the same ways as the original pWEB vector, but offers additional features that can be used if and when desired. For example, any of the cosmid clones produced in the pWEB::TNC vector can be used for subsequent generation of a random unidirectional deletion library from any chosen cosmid clone when used in the conjunction with the pWEB::TNC Deletion Cosmid Transposition Kit.
High efficienciesthe unique preparation of MaxPlax Extracts result in packaging efficiencies of up to 3 x 10 9 pfu/g DNA. MaxPlax Extracts are devoid of all known restriction activities. Packages highly methylated DNA as efficiently as unmethylated DNA.
pWEB::TNC Deletion Cosmid Transposition Kit

The pWEB::TNC Cosmid Cloning Kit and pWEB::TNC Deletion Cosmid Transposition Kit are used in concert to produce a complete random deletion library from any primary cosmid clone. A complete and unbiased primary cosmid library can be constructed easily and reproducibly in about two days using the pWEB::TNC Cosmid Cloning Kit. Once the primary cosmid library is produced, the pWEB::TNC Deletion Cosmid Transposition Kit can be used to generate and produce a set of random unidirectional deletion clones. The deletion library is produced simply by incubating any chosen primary cosmid clone with EZ::TN Transposase for 2 hours to overnight at 37C and then transforming competent E. coli . Cat. No. PC8805 WEBC931 WEBC942 Product pWEB Cosmid Cloning Kit* pWEB::TNC Cosmid Cloning Kit* pWEB::TNC Deletion Cosmid Transposition Kit** pWEB::TNC Cosmid Vector Qty 1 Kit 1 Kit 10 rxns
Cat. No. MP5105 MP5110 MP5120
Product MaxPlax Lambda Packaging Extracts MaxPlax Lambda Packaging Extracts MaxPlax Lambda Packaging Extracts
Qty 5 ext. 10 ext. 20 ext.
Contents: Pre-dispensed MaxPlax Extracts, Control Lambda DNA, Control Host Cells ( E. coli)
TNC9401
10 g
*1 Kit is sufficient for producing up to 10 complete and unbiased cosmid libraries. Contents: pWEB or pWEB::TNC Cosmid Vector, End-repair Enzyme Mix, Endrepair 10X Buffer, dNTP Mix, Fast-Link DNA Ligase Fast-Link 10X Ligation Buffer, ATP Solution, GELase Gel-Digesting preparation, GELase 50X Reaction Buffer, MaxPlax, Lambda Packaging Extracts, Ligated Lambda Control DNA, 40-Kb T7 Control DNA, EPI100 Plating Strain, Control Lambda Plating Strain **Contents: EZ::TN Transposase, EZ::TN 10X Reaction Buffer, EZ::TN 10X Stop Solution, Unlabeled Sequencing Primer, Control Cosmid with 40-kb Insert, Sterile Water
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Screening Colonies/ Electroporation Cuvettes

Epi-Grids Colony Grid Templates
Epi-Grids Colony Grid Templates are easy-to-use griding templates that allow rapid organization of colonies from initial screening plates and for preparing precise master plates. Epi-Grids Colony Grid Templates are made of flexible clear plastic and have a strip of pressure-sensitive adhesive that allows the grid sheet to adhere to plastic Petri plates, thus ensuring correct alignment of colonies on subsequent secondary plates.
CLONING
Screening and Electroporation
Colony Fast-Screen Kit (Size Screen)

The Colony Fast-Screen Kit (Size Screen), the original Colony FastScreen Kit, provides a rapid and sensitive method for screening the size of cloned DNAs without the need to grow cultures or perform minipreps or restriction endonuclease digestions. The size of most clones can be determined in 1 hour or less. Bacterial Artificial Chromosome (BAC) clones can be estimated in as little as 4 hours. Benefits Rapiddetermine the size of PCR, cDNA, and other clones in 1 hour and of BAC clones in as little as 4 hours without the need to grow cultures or perform restriction digests. Sensitivethe size of single-copy BAC clones are readily estimated. High-throughput capabilitythe kit is amenable to both highthroughput and routine cloning applications. Flexiblecan be used with all standard E. coli host strains. Product Colony Fast-Screen Kit (Size Screen)* Qty 1 Kit
Cat. No. FS08250
*Reagents are sufficient to green 200 colonies. Contents: EpiLyse Solution, EpiBlue Solution
Colony Fast-Screen Kit (PCR Screen)

The Colony Fast-Screen Kit (PCR Screen) provides a rapid method for preparing clones for screening by PCR. Using the Colony Fast-Screen Kit (PCR Screen) there is no need to grow cultures or purify DNA prior to PCR. The kit can be used with all standard E. coli hosts and all cloning vectors. Thermostable polymerase and PCR primers are not provided. Benefits Benefits Rapididentify desired clones by PCR without the need to grow cultures or purify DNA. Sensitiveenables PCR screening of high-copy clones, singlecopy clones, and bacterial genomic DNA. High-throughput capabilitythe kit is amenable to both highthroughput and routine cloning applications. Flexibleenables both long and short PCR amplifications and can be used with all standard E. coli host strains. Cat. No. FS0322H Product Colony Fast-Screen Kit (PCR Screen)** Qty 1 Kit

Cat. No. EG04850
Easy and convenient - Simply pull off the protecting adhesive covering and attach to the bottom of any 10- centimeter petri dish. Two patterns - Epi-Grids Templates are available with either 24 or 48 squares. Product Epi-Grids Colony Grid Templates - 24 Squares Qty 50 Temp
EG048100 EG0485H EG04810H
Epi-Grids Colony Grid Templates - 24 Squares 100 Temp Epi-Grids Colony Grid Templates 48 Squares Epi-Grids Colony Grid Templates 48 Squares 50 Temp 100 Temp
Reagents are sufficient to screen 200 colonies **Contents: PCRLyse Solution, Gel Loading Solution
EPICENTREs electroporation cuvettes are manufactured to strict quality standards to ensure consistent pulse delivery and reproducible results. Electroporation cuvettes are available in 1 mm and 2 mm gap widths. Compatible with most electroporation instruments including Eppendorf, Bio-Rad, BTX, Invitrogen and others. Cat. No. EC01091 EC01082 Product Electroporation Cuvettes, 1 mm Electroporation Cuvettes, 2 mm Qty 1 bag (50 pcs.)
Colony Fast-Screen Kit (Restriction Screen)

The Colony Fast-Screen Kit (Restriction Screen) provides an easy and rapid method to screen the size and orientation of cloned DNA. There is no need for overnight cultures or plasmid minipreps. The Colony FastScreen Kit Restricti-Lyse solution lyses cells in an environment, which is not inhibitory to restriction enzyme activity. Recombinant clone-containing E. coli colonies can be screened in 25 minutes using the standard protocol, or in as little as 10 minutes with the accelerated protocol. Cat. No. FS0472H 1 bag (50 pcs.) Product Colony Fast-Screen Kit (Restriction Screen)# Qty 1 Kit
Other electroporation cuvettes also available. Pleae view page # 73
# Contents : Restricti-Lyse Solution & Gel Loading Solution. Sufficient reagents to screen 200 colonies.
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Competent Cells for PCR & Genomic Cloning

All EPICENTRE Competent Cells have the following features :
Endonuclease minus ( endA 1 ) lac Z M15 Endonucelease A is a DNase that can survive boiling lysis and can degrade plasmid DNA, reducing the DNA yield from plasmid purifications. A deletion in the lac Z gene, which codes for part of the amino-terminal of the -galactosidase enzyme. A plasmid containing sequences of the amino terminus can complement this deletion to give functional -galactosidase. When X-gal is used as the substrate, -galactosidase produces a blue product; the colonies of these cells appear blue. When the complimentary sequence on the vector is disrupted by inserting a cloned fragment of DNA, the enzyme is no longer functional and cannot produce a blue product; the colonies of these cells are white. On a plate of mixed blue and white colonies, the white colonies should contain the cloned DNA. The recA gene codes for a DNA-dependent ATPase that is essential for genetic recombination in E. coli . Bacterial strains deficient in recA are defective in recombination. Plasmids in these strains are maintained as monomers (do not form multimeric circles) and are less likely to incur deletions. Restriction systems are designed to protect bacterial cells from foreign DNA. Deleting the host cells restriction system allows efficient cloning of DNA with different methylation patterns, such as DNA found in mammals, higher plants, and many prokaryotes
CLONING
Competent Cells
Recombination minus (recA 1 ) Restriction minus (mcr A, [mrrhsdRMS-mcrBC ])
Electrocompetent Cells
Strain Name T1 & T5 Phage Resistant Catalog Number EC10005 5 x 100 l EC10010 10 x 100 l EC0205T1 5 x 100 l EC0210T1 10 x 100 l ECP09500 5 x 100 l Transformation Efficiency (cfu/g pUC19) Used with Specific Vector Ori Accepts Large DNA Applications
TransforMax EC100
No
>1 x 10 10
No
145 kb
General Purpose
TransforMax EC100-T1 R
Yes
>1 x 10 10
No
145 kb
General Purpose
TransforMax EC100Dpir+
No
>5 x 10 9
R6K ori 15 copies/cell
Upto 100 kb
Rescue cloning using EZ-Tn5 or HyperMu Transposome Complexes Rescue cloning using EZ-Tn5 or HyperMu Transposome Complexes Use with CopyControl Vectors for inducible single to multiple copies/cell Clone toxic genes and unstable DNA Use with CopyControl Vectors for inducible single to multiple copies/cell Clone toxic genes and unstable DNA Controls copy number of common vectors Clone toxic genes and unstable DNA
TransforMax EC100D pir116
No
EC6P095H 5 x 100 l
>5 x 10 9
R6K ori 250 copies/cell
Upto 50 kb
TransforMax EC1300
No
EC300105 5 x 100 l EC300110 10 x 100 l EC300150 50 x 100 l
>1 x 10 10
Vectors containing both F-factor and ori V replicons
At least 145 kb Good for Libraries
TransforMax EPI300-T1 R
Yes
EC02T15 5 x 100 l EC02T110 10 x 100 l
>1 x 10 10
Vectors At least 145 containing both kb Good for F-factor and ori V Libraries replicons
CopyCutter EPI400
Yes
C400EL10 10 x 50 l
>1 x 10 10
ColE1-type replicons
145 kb
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Competent Cells for PCR & Genomic Cloning

Chemically Competent Cells
T1 & T5 Phage Resistant No Yes Catalog Number CC02810 10 x 50 l CCT10210 10 x 50 l Transformation Efficiency (cfu/g pUC19) >1 x 10 8 >5 x 10 7 Used with Specific Vector Ori No Accepts Large DNA At least 23 kb At least 23 kb
CLONING
Competent Cells
Strain Name TransforMax EC100TM TransforMax EC100-T1 R
Applications
General Purpose General Purpose Use with CopyControl Vectors for inducible single to multiple copies/cell Clone toxic genes and unstable DNA Use with CopyControl Vectors for inducible single to multiple copies/cell Clone toxic genes and unstable DNA Controls copy number of common vectors Clone toxic genes and unstable DNA
No
TransforMax EPI300
No
C300C105 10 x 50 l
>5 x 10 8
Vectors containing both F-factor and oriV replicons
At least 23 kb
TransforMax ECI300-T1 R
Yes
CT1C0210 10 x 50 l
>5 x 10 8
Vectors containing both F-factor and oriV replicons
At least 23 kb
CopyCutter EPI400
Yes
C400CH10 10 x 50 l
>1 x 10 7
ColE1-type replicons
At least 23 kb
Cat. No. EC10005 EC10010 CC02810 EC0205T1 EC0210T1 CCT10210
Product TransforMax EC100 Electrocompetent E. coli TransforMax EC100 Electrocompetent E. coli TransforMax EC100 Chemically Competent E. coli TransforMax EC100-T1R Electrocompetent E. coli TransforMax EC100 Electrocompetent E. coli TransforMax EC100-T1R Chemically Competent E. coli Product TransforMax EPI300 Electrocompetent E. coli* TransforMax EPI300 Electrocompetent E. coli* TransforMax EPI300 Electrocompetent E. coli* TransforMax EPI300 Chemically Competent E. coli* CopyControl Induction Solution TransforMax EPI300-T1R Electrocompetent E. coli** TransforMax EPI300-T1R Electrocompetent E. coli** TransforMax EPI300 T1R Chemically Competent E. coli**
Qty 5 x 100 l 100 x 100 l 10 x 50 l 5 x 100 l 100 x 100 l 10 x 50 l
CopyCutter EPI400 Electrocompetent E. coli CopyCutter EPI400 Chemically Competent E. coli

CopyCutter EPI400 E.coli* cells were developed to significantly lower the copy number of a wide variety of common vectors so that you can more readily clone unstable DNA sequences. DNA that is unstable at highcopy number often codes for a protein that inhibits cell growth or contains AT- and GC-rich sequences or sequences with strong secondary structure. The CopyCutter EPI400 cell line was derived from our hightransformation efficiency phage T1-resistant TransforMAX EC100 E.coli strain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC- and pET-type vectors). This constitutively expressed gene, pcnB (plasmid copy number), was deleted from the TransforMAX EC100 strain and replaced with a modified pcnB gene that is linked to an inducible promoter, creating the CopyCutter EPI400 strain. The copy number of ColE1-type vectors in the CopyCutter EPI400 strain compared to the parental TransforMAX EC100 strain is approximately 4to 25-fold lower, depending on the vector. Moreover, following a short incubation in the presence of the CopyCutter Induction Solution, you can increase the copy number of the vector to improve plasmid yields.
Cat. No. EC300105 EC300110 EC300150 C300C105 CCIS125 EC02T15 EC02T110 CT1C0210
Qty 5 X 100 l 10 X 100 l 50 X 100 l
Cat. No. 10 X 50 l 25 ml 5 X 100 l 10 X 100 l 10 X 50 l C400CH10 C400EL10
Product CopyCutter EPI400 Electrocompetent E.coli CopyCutter EPI400 Chemically Competent E.coli CopyCutter Induction Solution (1000x)
Qty 10 X 50 l
10 X 50 l
CIS40025
25 ml
Includes CopyCutter Induction Solution and pUC19 Control DNA.
*Includes CopyControl Induction Solution and pUC19 control DNA. **Includes pUC19 Control DNA
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Mammalian cDNA Libraries and Clones

We offer an extensive library of mammalian cDNA clones, including complete full-length cDNAs, partial full-length cDNAs, ESTs, and ORF-only clones.
cDNA LIBRARIES AND CLONES

Ready Clones
Human Freedom ORFs

Freedom ORF clones are collections of high quality sequence verified genes cloned into vectors that are free from the IP constraints commonly found with other recombinational cloning systems. Each clone has been amplified and sequenced to ensure the highest quality standard.
Mammalian Gene Collection (MGC) the gold standard collection of full-length cDNAs for human, mous, and rat
Every MGC clone is fully sequenced and verified to contain a complete open reading frame (ORF). Full-length cDNAs provide the strongest evidence for the structure of protein-encoding mRNA transcripts and are indispensable tools for determining gene function. MGC is the largest single collection of confirmed full-length and annotated cDNAs. The MGC is generated by a consortium of leading, NIH-funded laboratories and is available to all without restriction.
BD Creator Universal Clones

Open Biosystems offers a set of over 1,100 human ORF clones, the BD Creator Universal Clones, which represents the initial release of the Freedom ORF collection. The BD Creator system is a flexible recombinational cloning system based on Cre-loxP reactions. These ORF clones are provided to you in the pDNR-Dual vector, allowing you to move the inserts directly into any expression vector of choice through a simple Cre recombinase reaction. Although Freedom ORF clones can be subcloned into any expression vector using traditional restriction enzyme and ligase cloning methods, the Creator System allows the target gene to be rapidly transferred in-frame to a variety of expression vectors. The BD Creator Universal Clones are constructed for immediate 3' tagging with a 6xHN affinity tag. The BD Creator System transfers a gene from a specialized Donor vector into any Creator-compatible expression vector, called an Acceptor Vector. The Cre-LoxP recombination reaction transfers the Freedom ORF directionally to the Acceptor vector without the loss or addition of sequence. The resulting expression clone contains the ORF in both proper orientation and reading frame. A variety of BD Creator-compatible expression vectors are commercially available or you can adapt the vector of your choice to the BD Creator System using a 161-bp cassette.
Assay-Ready MGC cDNA Discover novel gene functions with genome-scale overexpression screening
Assay-Ready MGC cDNA draws on the gold standard Mammalian Gene Collection (MGC) to provide you with over 16,000 verified full-length human and mouse cDNAs cloned into the mammalian expression vector pCMV-SPORT6. DNA is spotted in 384-well microplates provided in duplicate, allowing you to begin screening without first performing thousands of plasmid preps. Easy protocols for cDNA overexpression assays are provided as well as additional cDNA plates for assay optimization.
BioTrack Human Gene Subsets

These subsets focus on well-known human gene families as well as important cellular pathways and processes. Each subset includes 3 parallel components: full-length cDNA clones (from the Mammalian Gene Collection and the Incyte Gene Collection), Human ORF clones, and Expression Arrest shRNA clones that enable the use of alternative or complementary strategies for determining gene function. For example, overexpression with full-length cDNA clones can be used to confirm the specificity of RNAi knockdown in a screen with Expression Arrest shRNA. Developed by Marc Vidal and coworkers, the BioTrack Human ORF clones contain ORF-only sequences (full-length clones with UTRs removed) derived from the MGC and cloned into a recombinational entry vector. These clones can be used in a wide range of applications including yeast two-hybrid screens, protein imaging in live cells, cellular and in vitro assays, and protein overexpression.and purification.
Incyte Gene Collection (IGC)

cDNAs for human, rat, monkey, and dog As a complement to the MGC, we offer the largely untapped Incyte collections for human, rat, monkey, and dog. A wide range of tissues are represented in each collection and together they contain over one million complete full-length cDNAs, partial full-length cDNAs, and ESTs. Complete full-length cDNAs have been full-insert sequenced and contain a complete ORF. Partial full-length cDNAs have been full-insert sequenced, but do not contain a complete ORF. While not fully annotated, these collections may be mined by BLASTing query sequences against our Incyte clone databases.
Mammalian IMAGE ESTs

The IMAGE consortium was formed in 1993 to provide a public resource to aid in gene discovery that would be free of patents/royalties and other restrictions. The IMAGE clone collection provides the community a resource of arrayed cDNA clones from various research efforts. The clones are from oligo dT-primed, directionally cloned plasmid cDNA libraries from human, mouse, rat, zebrafish, Xenopus and primate sources. The Cancer Genome Anatomy Project (CGAP) was formed in 1996 to investigate genes associated with cancerous cells at the molecular level. As a result, CGAP is responsible for thousands of expressed sequence tags (ESTs), which are continuously added to the IMAGE collection.
Human ORF Collection

The Center for Cancer Systems Biology of the Dana-Farber Institute has created a path-breaking collection of ORF-only clones (full-length cDNAs with UTRs removed) beginning with over 8000 genes 1 and with future updates planned. These clones are derived from fully sequenced Mammalian Gene Collection (MGC) full-length cDNAs and cloned into a recombinational entry vector. ORF clones provide a shortcut to protein expression, allowing you to skip PCR, cloning into an expression vector, and verifying the ends of the ORF DNA sequence. Each CDS was amplified for only 25 cycles with gene-specific primers and KOD HiFi Polymerase, greatly minimizing the risk of PCR-induced mutations. The Gateway entry vectors ensure easy transfer into prokaryotic, mammalian, viral, or insect expression systems. For maximum flexibility, the ORF stop codon has been removed.
Other ESTs
Human Cystic Fibrosis Collection Human Eye ESTs Mouse BMAP cDNA Mouse NEIBank cDNA Clones Mouse NIA cDNA Clones Mouse Organ of Corti Collection Mouse Retina cDNA Clones Rat Non-Redundant cDNAs Porcine ESTs
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Mammalian and Non Mammalian cDNA Libraries and Clones

Brain cDNA Libraries
Average insert sizes range from 2.1-2.3kb and all four libraries contain 100% recombinants. The vector used to construct these libraries is Express 1 and the product shipped contains DH10B in SOC plus ampicillin and 20% glycerol. Tissue for these libraries originated from various sources: Human Adult Brain Library - Brain tissue from three male donors aged 23-27 years old Human Fetal Brain Library - Brain tissue from one 24 week old male donor Mouse Brain Library - Brain tissue from 8 week old male C57Bl/6J mice Rat Brain Library - Brain tissue from 8 week old, male Sprague-Dawley rats

Ready Clones
Zebrafish cDNA Library

The amplified version of the Danio rerio primary library used to create the arrayed I.M.A.G.E. libraries NIH_ZGC_10 and NIH_ZGC_7 (normalized). Inserts were directionally cloned using oligo(dT) primers and were ligated at the Eco RV and Not I sites.
Zebrafish Gene Collection (ZGC)

The Zebrafish Gene Collection (ZGC) is a complete set of full-length cDNA clones of expressed genes for zebrafish. Each clone is supplied in a plasmid vector transformed into E. coli .
Zebrafish IMAGE cDNAs

The IMAGE clone collection provides the community a resource of arrayed cDNA clones from various research efforts. The clones are from oligo dT-primed, directionally cloned plasmid cDNA libraries from human, mouse, rat, zebrafish, Xenopus and primate sources. The Cancer Genome Anatomy Project (CGAP) was formed in 1996 to investigate genes associated with cancerous cells at the molecular level. As a result, CGAP is responsible for thousands of expressed sequence tags (ESTs), which are continuously added to the IMAGE collection.
Incyte cDNA Libraries

This unique resource contains human, mouse, canine, wheat, rice, potato, Arabidopsis, corn and primate cDNA libraries. Nearly 400 Incyte cDNA libraries are available from a wide range of tissues. Libraries are provided as plasmids to facilitate transportation and storage. Upon receipt, libraries should be transformed into ultrapure electrocompetent cells.
NON MAMMALIAN cDNA cDNA from Bacteria Brucella ORF Collection

Created by a consortium of laboratories, the Brucella ORF Collection is comprised of 3091 ORFs that have been cloned into a Gateway entry vector and are ready to be transferred into Gateway destination vectors for protein expression and functional analysis. For example, protein-protein interactions can be studied by transferring the collection to bait and prey plasmids for yeast two-hybrid screening or to a destination construct that expresses each ORF with an affinity, epitope, or fluorescent tag.
Zebrafish IMCB Collection

Singapores Institute for Molecular and Cell Biology (IMCB) has isolated over 15,000 unique genetic markers from the zebrafish (Danio rerio). A total of 15,590 unique zebrafish EST clusters from two cDNA libraries (primary and normalized) have been identified. This EST set is complementary to the existing ESTs in the public database and will be invaluable in assisting in the annotation of genes based on the upcoming zebrafish genome sequence.
Zebrafish Morpholino Collection

The Zebrafish Morpholino Collection contains hundreds of oligos designed and synthesized to rapidly reduce gene expression levels. Each sequence is designed to block the translational start site of the targeted gene. As morpholinos are synthesized synthetically and are nuclease resistant, the expression level reduction is specific and prolonged. Each genes start site is identified and a morpholino is produced that is complementary to ~25 bases -25 to +25 bases from the start.
C. jejuni ORF Collection

Jodi Parrish et al from the Center for Molecular Medicine and Genetics, and Department of Biochemistry and Molecular Biology, Wayne State University have cloned 1,619 ORFs from Campylobacter jejuni into E. coli using in vivo recombination. E. coli SURE clones containing the C. jejuni ORFs in the vector pTLJ03 are available. pTLJ03 is an expression vector that generates N-terminal GST-His-tagged fusion proteins from the C. jejuni ORF set.
Zebrafish NEIBank cDNA Clones Drosophila Gene Collection (DGC)

The Berkeley Drosophila Genome Project has now analyzed over 240,000 Drosophila ESTs. Over 10,000 non-redundant cDNA clones, covering most of the predicted 13,601 genes in the Drosophila genome are now available and comprise the Drosophila Gene Collection (DGC).
cDNA from FISH Fugu IMAGE cDNAs Stickleback cDNA Collections

The three-spine stickleback ( Gasterosteus aculeatus ), a teleost fish, is an evolutionary model for morphological change that is growing in popularity. (It was mentioned under Sciences Breakthrough of the Year for 2005.) We offer 6 collections of cDNAs originating from specific tissues in different geographical populations.
Xenopus IMAGE cDNAs

The clones are from oligo dT-primed, directionally cloned plasmid cDNA libraries from human, mouse, rat, zebrafish, Xenopus and primate sources. The Cancer Genome Anatomy Project (CGAP) was formed in 1996 to investigate genes associated with cancerous cells at the molecular level. As a result, CGAP is responsible for thousands of expressed sequence tags (ESTs), which are continuously added to the IMAGE collection.
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Yeast/ C.elegans cDNA Libraries and Clones

cDNA from C.elegans C. elegans ORF Collection
Dr. Marc Vidals laboratory at the Dana-Farber Cancer Institute has generated the C. elegans Open Reading Frame (ceORF) clones representing over 10,000 worm genes. The ceORFs were generated by amplification from full length cDNA libraries and cloning into the Gateway 2 recombinational cloning system (pDONR201). ceORF clones can be subcloned into any expression vector, using traditional restriction enzyme and ligase cloning methods. Alternatively, the recombinational cloning system allows the target gene to be rapidly transferred in-frame into a variety of expression vectors. Release 1.1 clones represent a pool of cloned products, thus multiple splice variants are preserved in each pool. ceORFs will be available in two different formats: glycerol stocks and spotted onto IsoCode cards.

Ready Clones
Yeast Insertional Mutant Strains

The Yeast Insertional Mutant collection contains over 3,600 mutagenized yeast strains providing a set of gene disruption mutants for phenotypic analysis. The mutagenized strains were developed via transposon insertional mutagenesis. The insertion of the transposon into the open reading frame (ORF) of the gene typically disrupts gene function, or insertion upstream or downstream of the ORF may result in mis-expression of the gene. The 6kb mTn contains the reporter gene lacZ , flanked by lox sites, and a 3xHA tag. The lacZ gene is lacking both its promoter and start codon, thus, the expression is dependent on the transposon being inserted in-frame into a gene and subsequently transcribed and translated.
Yeast Knockout Strains

The Saccharomyces Genome Deletion Project has developed a unique collection of knock-out strains covering 96% of the yeast genome. This collection of over 6,000 gene-disruption mutants provides a unique tool for the functional analysis of the yeast genome. The inclusion of distinct tags - or molecular barcodes - that identify each strain allows phenotypic analysis to be performed on a single gene basis or a genome-wide scale. By using the yeast knock out strains for functional profiling, one can also gather much information about human gene function by analogy. While >6000 represents the number of individual genes, there are four backgrounds (haploids of MATa and MATalpha, heterozygous diploid, and homozygous diploid) with many genes available in more than one background, thereby creating over 20,000 knock out strains.
C. elegans ORF-RNAi Collection

The C. elegans ORF-RNAi Collection includes clones containing full open reading frames for over 11,000 genes, ready for your RNAi screens! These clones are derived from the C. elegans ORFeome Library v1.1. The C. elegans ORF-RNAi Library provides you comprehensive coverage for RNAi screening. Full Coding Regions: From Start-to-Stop, the entire open reading frame is cloned into an RNAi feeding vector and compatible host. Feeding Ready: The cloned ORFs have been transformed into the RNAi feeding bacterial strain HT115(DE3) 2 and the feeding vector, PL4440. RNAi Versatility: Plasmid preps of the ORF-RNAi clones can be used as templates for in vitro dsRNA synthesis for soaking or injection
Yeast Magic Marker Strains

Genome-wide collections of yeast knockout (YKO) mutants have facilitated the systematic functional analysis of yeast genes. However, haploid and homozygous diploid YKOs experience strong selection for compensatory mutations and so the genetic quality of such strains deteriorates over time. Knockout phenotypes can be protected from selection by hiding the mutation as a heterozygote with the wild-type allele. However, it is then correspondingly difficult to detect the knockout phenotype.The Magic Marker technology allows fresh creation of haploid knockouts from heterozygous diploids in a high-throughput manner by employing a simple selection step following sporulation. Thus, a starting pool containing thousands of high-quality MATa haploid YKOs can be efficiently created.
C. elegans Promoter Collection

The C. elegans Promoter Collection from Open Biosystems includes clones containing the predicted promoters for over 6,000 genes designed to easily construct GFP reporter strains. Get ready to localize your protein expression patterns on a scale you never thought possible.
cDNA from Yeast Candida albicans Genomic Library

The library is ready for transformation into the amenable Saccharomyces cerevisiae to allow rapid, large-scale screens of C. albicans genes that can generate a phenotype of interest. The library is supplied as an E. coli culture. The average size of inserts is ~4.3 Kb and they have been cloned into pRS426 (2u, URA3), a S. cerevisiae high copy vector. The number of clones in the library is ~50,000 that covers the haploid genome 14-fold and greater than 97% of the clones contain inserts.
Yeast ORF Collection

The Yeast ORF Collection enables robust protein expression and purification for over 5500 S. cerevisiae genes. Each plasmid construct is available in yeast or E. coli format as a glycerol stock. Open reading frame (ORF) clones provide a shortcut to protein expression, allowing you to skip PCR, cloning into an expression vector, and verifying the DNA sequence for each construct. All yeast-format constructs have been verified by western blotting to express protein of the correct length. The optional E. coli format enables easy conversion to alternative yeast strains. The versatile 19-kDa tandem fusion tag enables a range of detection and affinity purification strategies. In addition to Protein A and 6xHis domains, it contains a hemagglutination (HA) domain.
HA-Tagged Yeast Strains

The HA-Tagged Yeast Collection contains over 2,400 yeast mutagenized strains each producing a single protein with an inserted triple hemagglutinin epitope tag (3xHA). The HA insertion potentially provides several means by which yeast protein function may be studied. Native amounts of protein can easily be purified from individual yeast strains using immunoprecipitation with a commercially available HA antibody. Also, HA insertion may potentially generate conditional alleles and hypomorphic mutants that exhibit partial gene function of particular importance in the analysis of essential genes.
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Yeast cDNA Libraries and BAC Clones

Yeast TAP Fusion Collection
To facilitate global protein analyses, Dr. Erin OShea and Dr. Jonathan Weissman (UCSF) have created a library where each ORF is tagged with a high-affinity epitope and expressed from its natural chromosomal location. Through immunodetection of the common tag, a resource now exists that provides a census of proteins expressed during log-phase growth and quantifies their absolute levels. The Yeast-TAP-Fusion Library allows the purification and selection of the entire yeast proteome and associated components using two simple affinity selection steps in tandem, enabling the development of a range of high-throughput functional assays. The C-terminal TAP insertion cassette contains the coding region for a modified version of the TAP (Tandem Affinity Purification) tag, which consists of a calmodulin binding peptide, a TEV cleavage site and two IgG binding domains of Staphylococcus aureus protein A, as well as a selectable marker. Background strain = MatA (BY4741).

cDNA/BAC Clones
Disease-specific Human Mapped BAC Collections

Break points in chromosomes, the evidence of which are deletions, insertions, inversions and translocations, have highlighted the role of genes in neoplasia. It was not long after researchers visualized these breakpoints that they identified non-random associations between these microscopic lesions and specific disease phenotypes. Disease-specific probe sets from Open Biosystems allow you access to collections of clones that span known breakpoints associated with a particular disease. These clones can be used on metaphase and interphase spreads using single or dual color FISH, as well as CGH to detect larger duplications, amplifications or deletions. Each set contains FISH mapped clones that have been precisely placed on human chromosomes at approximately 1-2 Mb intervals. This has enabled the identification and selection of BACs that either flank or encompass known breakpoints associated with cancer. B-cell (IgH) Leukemia & Lymphoma BAC Set available T-cell (TCR) Leukemia & Lymphoma BAC Set available Ewings Sarcoma BAC Set available
Yeast Tet-Promoter Hughes Strains

The function of many of the essential genes has yet to be defined. In part this is due to the difficulty of utilizing essential genes in functional assays. The Tet-promoters Hughes collection (yTHC) from the Hughes Laboratory, University of Toronto, helps to overcome this barrier. The yTHC collection contains 800 essential yeast genes for which expression is regulated by doxycycline. The endogenous promoter has been replaced with a Tet-titratable promoter in the genome. Thus, the expression of the gene can be switched off by the addition of doxycycline to the yeasts growth medium.
Mere Mouse Mapped BAC Collection

Dr. Julie Korenbergs laboratory at Cedars-Sinai Medical Center has created a resource of 156 FISH-mapped BAC clones spanning the mouse genome at an average resolution of 19 Mb. Forty-two of these clones are linked to the centromeric and telomeric ends of the Whitehead/MIT recombinational maps, while the remaining 114 clones have been assigned to single chromosome bands. This resource is the first collection to effectively integrate the cytogenetic and functional maps of the mouse genome. All clones were derived from the CITB mouse BAC library which was constructed from CJ7/129SV embryonic stem (ES) cells. All clones are in the pBeloBAC11 vector and the average insert size for the library is 130Kb. Orders for individual clones must be placed by BAC clone ID.
Incyte cDNA Libraries

Please view page #
FISH Mapped BAC Clones

The Cancer Chromosome Aberration Project (CCAP), a joint initiative supported by the NCI and NCBI, aims to develop tools that assist in the analysis of chromosomal aberrations associated with malignant transformation. To meet this objective, CCAP generated a set of cytogenetically FISH-mapped BAC clones at a resolution of 1-2Mb across the human genome. The BACs have been physically mapped to the genome using sequence tag sites (STS) and ordered using genetic linkage and/or radiation hybrid mapping data. Additionally, these clones are FISH mapped onto pro-metaphase spreads to allow high-resolution placement, uniform coverage of the chromosomes, and their precise positioning relative to one another. This resource is the first collection to effectively integrate the cytogenetic and sequence maps of the human genome, thus facilitating the transition to molecular cytogenetics. To find an individual BAC clone covering a particular region: Point your browser at: http://cgap.nci.nih.gov/Chromosomes/ CCAP_BAC_Clones
CalTech Human and Mouse BAC Clones
GenMap Human BAC Collection

A genome wide collection of evenly distributed mapped BAC clones has been made available by Dr. Vivian Cheungs lab at the University of Pennsylvania. Beginning with the RPCI-11 human male BAC library, STS markers were used as anchors to map the BACs to the genome, at ~1Mb resolution. A subset of these clones were used in the NCI CCAP project and the results of the FISH analyses performed by the NCI CCAP on these clones are available at both the GenMapDB and the CCAP web pages. To find a BAC clone covering a particular region: Point your browser at: http://genomics.med.upenn.edu/genmapdb/
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In-vitro Transcription, RNA polymerases

T7, T3, and SP6 RNA Polymerases
T7, T3, and SP6 RNA Polymerases produce defined RNA by in vitro transcription of double-stranded DNA that is downstream of the respective RNA polymerase promoter. It has extremely high promoter specificity and is the best value in phage RNA polymerases. Applications RNA synthesized can be used as a hybridization probe, antisense RNA, a ribozyme, a template for in vitro translation, as a precursor mRNA for splicing or other processing studies, or to make dsRNA for RNA interference or gene silencing. Synthesis of RNA for nucleic acid amplification methods or gene expression studies. A buffer package containing 5X Transcription Buffer and DTT may be purchased separately.
IN VITRO TRANSCRIPTION
Prokaryotic Polymerases
E. coli RNA Polymerase Core Enzyme and Holoenzyme (Sigma-Saturated)

Both the Core Enzyme and the Holoenzyme preparations of E. coli RNA Polymerase are isolated from the rifampicin-sensitive strain BL21. EPICENTRE is the only company that offers purified Core Enzyme, which has no detectable sigma subunit, and 100% sigma-saturated (s70)-Holoenzyme. The Core Enzyme is useful in studying mechanisms of transcription initiation, since it will not initiate specific transcription at promoter sequences on bacterial or bacteriophage DNA due to a lack of sigma factor. However, the Core Enzyme will generate a variety of nonspecific transcripts. The sigma-saturated Holoenzyme is very efficient in specifically transcribing a variety of double-stranded DNA templates containing promoters.
Cat. No. C90100 C90250 C90500 S90050
Product E. coli RNA Polymerase Core Enzyme E. coli RNA Polymerase Core Enzyme E. coli RNA Polymerase Core Enzyme E. Coli RNA Polymerase Holoenzyme (Sigma-Saturated) E. Coli RNA Polymerase Holoenzyme (Sigma-Saturated) E. Coli RNA Polymerase Holoenzyme (Sigma-Saturated)
Qty 100 U 250 U 500 U
Cat. No. T7905K T7925K T7950K TM910K TM925K TM950K TH925K TH950K TU950K T4905K T9050K TM4910K TH4950K S4905K S4950K SM910K BP1001
Product T7 RNA Polymerase 50 U/l T7 RNA Polymerase 50 U/l T7 RNA Polymerase 50 U/l T7 RNA Polymerase 200 U/l T7 RNA Polymerase 200 U/l T7 RNA Polymerase 200 U/l T7 RNA Polymerase 1,000 U/l T7 RNA Polymerase 1,000 U/l T7 RNA Polymerase 2,500 U/l T3 RNA Polymerase 50 U/l T3 RNA Polymerase 50 U/l T3 RNA Polymerase 200 U/l T3 RNA Polymerase 1,000 U/l SP6 RNA Polymerase 50 U/l SP6 RNA Polymerase 50 U/l SP6 RNA Polymerase 200 U/l Transcription Buffer Package (5X Transcription Buffer and 2.5 ml of 100 mM DTT.)
Qty 5,000 U 25,000 U 50,000 U 10,000 U 25,000 U 50,000 U 25,000 U 50,000 U 50,000 U 5,000 U 50,000 U 10,000 U 50,000 U 5,000 U 50,000 U 10,000 U 5 ml
50 U
S90100
100 U
S90250
250 U
Thermus Thermostable RNA Polymerase

Derived from the thermophilic bacterium, Thermus thermophilus , Thermus Thermostable RNA Polymerase is the only commercially-available RNA polymerase that is stable and has optimal activity at temperatures above 65C. Thermus RNA Polymerase resembles E. coli RNA polymerase in subunit structure. Cat. No. T90050 T90100 T90250 Product Thermus Thermostable RNA Polymerase Thermus Thermostable RNA Polymerase Thermus Thermostable RNA Polymerase Qty 50 U 100 U 250 U
Q-Beta Replicase
Q-Beta Replicase (Q Replicase) is an RNA-directed RNA polymerase that is responsible for replication of the coliphage Q-Beta RNA genome. Various aspects of the enzyme are presented in a review by Blumenthal and Carmichael. 1It is composed of four subunits, one of which is encoded by the Q-Beta phage and three by the E. coli host. EPICENTREs Q-Beta Replicase is purified from E. coli containing a plasmid that expresses the phage-encoded subunit. All four enzyme subunits are present in equal proportions. Cat. No. Product Q-Beta Replicase 1 U/l* Qty 250 U
T7 & SP6 R & DNA Polymerases

Cat. No. D7P9201K D7P9205K D6P9301K D6P9305K Product T7 R & DNA Polymerase 50 U/l T7 R & DNA Polymerase 50 U/l SP6 R & DNA Polymerase 50 U/l SP6 R & DNA Polymerase 50 U/l Qty 1000 U 5000 U
QB501250 1000 U 5000 U
*Enzyme only, 10x TA Reaction buffer and NTPs are available separately.
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Make RNA transcript from ssDNA, Synthesize RNA probes

MiniV In Vitro Transcription Kit
MiniV In Vitro Transcription Kit provides MiniV RNA Polymerase* (MiniV RNAP) and all other components for in vitro transcription of RNA using single-stranded DNA (ssDNA) templates that are functionally linked to a single-stranded bacteriophage N4 promoter. MiniV RNAP is a transcriptionally-active 1,106-amino acid domain of the N4 virion RNA polymerase. Since it lacks RNA strand-displacement or unwinding activity on RNA:DNA hybrids, MiniV RNAP requires E. coli Single-Stranded Binding Protein (EcoSSB Protein) to displace the RNA transcript from the DNA template strand for efficient in vitro transcription. Like other RNA polymerases, MiniV RNAP can also synthesize concatameric RNA by rolling circle transcription of circular single-stranded DNA templates. Phage N4 transcription promoters used by MiniV RNAP are characterized by conserved sequences and a 5-basepair stem, 3-base loop hairpin structure.
Probe synthesis, RNA Amplification
AmpliScribe T7-Flash Transcription Kit AmpliScribe T3-Flash Transcription Kit

The AmpliScribe T7- Flash and AmpliScribe T3- Flash Transcription Kits, EPICENTREs new in vitro transcription kits, produce more RNA in 30 minutes than other in vitro transcription kits produce in two hours. AmpliScribe T7- Flash and T3- Flash Kits utilize proprietary enzyme formulations that enable the maximum possible yields of RNA from virtually any template. The template can be any sequence, whether long or short, that is downstream of the respective T7 or T3 RNA polymerase promoter, including sequences in linearized plasmids, cDNA, double-stranded oligos or PCR products. Benefits Exceptionally high yields of full-length RNA. A 20-l reaction with 1 g of control DNA template yields 160 to 180 g (8 to 9 mg/ml) of full-length 1.4-kb RNA in 30 minutes. Fast. When transcribing 1 g of linearized plasmid DNA template, a standard AmpliScribe T7- Flash or T3- Flash reaction is complete in 30 minutes. High yields with both long and short templates - even with templates smaller than 30 bp. Theres no need for a special kit to make short RNA. AmpliScribe T7- Flash and AmpliScribe T3- Flash Kits produce high yields of active ribozymes, short hairpin RNA (shRNA), and more. Reactions are easily modified to incorporate nonradioactive, biotin, aminoallyl-labeled NTPs, or cap analogs. RNase inhibitor is included in the AmpliScribe T7- Flash and T3- Flash Enzyme Solutions to ensure RNA integrity. Can be used for Eberwine-type RNA amplification procedures. However, we recommend TargetAmp Aminoallyl-aRNA Amplification Kits for amplification of mRNA from laser capture microdissection (LCM) or other small samples. Product AmpliScribe T7-Flash Transcription Kit AmpliScribe T7-Flash Transcription Kit AmpliScribe T7-Flash Transcription Kit AmpliScribe T7-Flash Transcription Kit AmpliScribe T7-Flash Transcription Kit Qty 5 rxns 25 rxns 50 rxns 25 rxns 50 rxns
Cat. No. MV41025
Product MiniV In Vitro Transcription Kit
Qty 25 rxns
RiboScribe RNA Probe Synthesis Kits

RiboScribe T7, T3, and SP6 Probe Synthesis Kits are convenient, reliable, and cost-effective kits for synthesis of high-specific-activity radioactive RNA probes for Southern and Northern blots, in situ hybridization, RNase protection assays, and other applications. Cat. No. RS71207 RS71203 RS71206 Product RiboScribe T7 RNA Probe Synthesis Kit RiboScribe T3 RNA Probe Synthesis Kit RiboScribe SP6 RNA Probe Synthesis Kit Qty 25 rxns 25 rxns 25 rxns
Cat. No. Enzyme solution contains RNase inhibitor. Control DNA template also provided. ASF3057 ASF3257 ASF3507 ASF03725 ASF03750
AmpliScribe T7 Aminoallyl-RNA Transcription Kit

The AmpliScribe T7 Aminoallyl-RNA Transcription Kit enables flexible high-yield synthesis of aminoallyl-labeled RNA. The kit utilizes EPICENTREs AmpliScribe T7 high-yield in vitro transcription system to incorporate 5-(3-aminoallyl)-UTP (AA-UTP) into the RNA transcript. Since AA-UTP is incorporated into RNA with the same efficiency as UTP, high yields of aminoallyl-labeled RNA are obtained. The aminoallyl-RNA product can then be reacted with amine-reactive biotinylation reagents or fluorescent dyes to obtain biotin-labeled or fluorescently-labeled RNA, respectively. Biotin-X-X-NHS is available from EPICENTRE for making biotinlabeled RNA, and amine-reactive dyes, such as N-hydroxy-succinimidyl (NHS) esters of fluorescent dyes, are commercially available from many sources. This method for making non-radioactively-labeled RNA is much more efficient and much less expensive than direct incorporation of fluorescent- or biotin-labeled NTPs into RNA during in vitro transcription. Applications Production of non-radioactive RNA probes for any purpose, including: - In situ hybridization experiments. - Blotting experiments. Product AmpliScribe T7 Aminoallyl-RNA Transcription Kit Qty 25 rxns
Enzyme solution contains Rnase Inh. Control DNA template also provided.
AmpliScribe T7, T3, and SP6 High Yield Transcription Kits

AmpliScribe T7, T3, and SP6 High Yield Transcription Kits are specially formulated to utilize high concentrations of NTPs that are inhibitory to other in vitro transcription systems. AmpliScribe High Yield Transcription reactions can produce >20-fold more full-length RNA transcript than conventional in vitro transcription reactions.
Cat. No. AS2607 AS3107
Product AmpliScribe T7 High Yield Transcription Kit AmpliScribe T7 High Yield Transcription Kit AmpliScribe T3 High Yield Transcription Kit AmpliScribe T3 High Yield Transcription Kit
Qty 25 rxns 50 rxns 25 rxns 50 rxns
Cat. No. AA50125
AS2603 AS3103
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Capped and tailed RNA

AmpliCap-Max T7 & T3 High Yield Message Maker Kits
EPICENTREs new AmpliCap-Max T7 and AmpliCap-Max T3 High Yield Message Maker Kits are specially formulated to produce the highest yield of 5'-capped RNA (m 7G[5']ppp[5']G cap analog) from an in vitro transcription reaction in the shortest reaction time. The new AmpliCap-Max T7 and AmpliCap-Max T3 High Yield Message Maker Kits feature: Yields up to 60 g of 5'-capped RNA per reaction. 30 minute reaction time. Up to 80% of the RNA is capped. An optimized AmpliCap-Max Cap/NTP PreMix, containing m7G[5']ppp[5']G Cap Analog and NTPs. A separate vial of GTP for efficient production of long, 5'capped RNA. Product AmpliCap-Max T7 High Yield Message Maker Kit AmpliCap-Max T3 High Yield Message Maker Kit Qty 25 rxns
Transcripts for Translation
If the capped RNA is to be used for translation studies, translational efficiencies can be much higher if the mRNA is also polyadenylated, especially if the mRNA is intended for use in vivo following transfection or microinjection. Cat. No. MM50110 Product MessageMAX T7 Capped Message Transcription Kit Qty 10 rxns
Contents: MessageMAX T7 Enzyme Solution (with added RNase Inhibitor), MessageMAX 10X Transcription Buffer, 2-Way Cap/NTP PreMix, 20 mM GTP, RNase-Free DNase I, DTT, RNase-Free Water, and Control Template DNA (linearized)
Standard Cap, 2-Way Cap, ARCA Cap, Unmethylated Cap and Trimethyl Cap Analogs
When a Cap Analog is substituted for a portion of the GTP present in an in vitro transcription reaction, up to ~80% of the transcripts will have a cap on their 5'-end. RNA prepared for microinjection into oocytes or for transfection into eukaryotic cells should be capped. Anti-reverse cap analogs (ARCAs), such as 3'-O-methyl-m7GpppG, were found to result in synthesis of transcripts that were more efficiently translated in vitro because the O-methyl group only permitted them to be incorporated in the correct orientation. Cat. No. C31010 C61025 C41210 C50110 C32010 C06005 Product Standard Cap Analog, m7G[5']ppp [5']G Solution Standard Cap Analog, m7G[5']ppp[5']G Solution 2-Way Cap Analog, m7G[5']pppp[5']m7G Solution ARCA Cap Analog, 3'-O-Methyl-m7G [5']ppp[5']G Solution Unmethylated Cap Analog, G[5']ppp[5']G Solution Trimethyl Cap Analog, m2,7,7G[5']ppp[5']G Solution Qty 500 nmoles 1250 nmoles 500 nmoles 500 nmoles 500 nmoles 250 nmoles
Cat. No. ACM04037
ACM04033
25 rxns
AmpliCap T7 & SP6 High Yield Message Maker Kits

AmpliCap T7 & SP6 High Yield Message Maker Kits are optimized to produce high yields of RNA having a standard m 7G[5']ppp[5']G cap analog on the 5'-end. Capping efficiencies approach 80%. Can be used for functional studies of heterogenous nuclear RNAs and viral RNAa. Benefits Yield up to 45 g and 35 g of RNA from 1g of T7 and SP6 control templates, respectively, in a 20-l reaction in 2 hours. Capping efficiencies up to 80%. An optimized m 7G[5']ppp[5']G Cap/NTP PreMix is provided for ease of use and highest yields of capped RNA transcripts. Separate vial of GTP is provided for efficient production of long, 5'-capped RNA. Formulated to utilize high concentrations of NTPs that are inhibitory to conventional in vitro transcription kits. Product AmpliCap T7 High Yield Message Maker Kit AmpliCap SP6 High Yield Message Maker Kit Qty 25 rxns
Cat. No. AC0707
Quantities of Cap Analogs are sometimes stated in absorbance units. One absorbance unit is equivalent to approximately 50 nmoles of Cap Analog.
A-Plus Poly(A) Polymerase Tailing Kit

A-Plus Poly(A) Polymerase uses ATP as a substrate for template-independent addition of adenosine monophosphate to the 3'-hydroxyl termini of RNA molecules. A-Plus Poly(A) Polymerase is encoded by an E. coli gene that has been cloned in a plasmid and overexpressed in an E. coli strain. Addition of a poly(A) tail to RNA synthesized in vitro in order to increase the stability of the RNA and enhance its ability to be translated in vivo after transfection or microinjection into eukaryotic cells. Addition of a poly(A) tail to an RNA molecule or a mixture of RNA molecules in order to provide a priming site for synthesis of first-strand cDNA using a primer with poly(dT) on its 3'-end portion. Cat. No. PAP5104H Product A-Plus Poly(A) Polymerase Tailing Kit (4 U/l) Qty 400 U
AC0706
25 rxns
MessageMAX T7 Capped Message Transcription Kit

Grudzien et al. recently demonstrated that in vitro-synthesized luciferase mRNA capped with the symmetrical cap analog, m 7Gp4m7G, was translated in a rabbit reticulocyte lysate system > 3-fold more efficiently than the same mRNA capped with the standard m7Gp3G cap analog. EPICENTREs new MessageMAX T7 Capped Message Transcription Kit gives the highest possible yield of capped RNA in just 30 minutes and, since it will have a 2-Way Cap, you should observe higher translational efficiencies using the 5'-capped RNA generated. Since the new 2-Way Cap included in the MessageMAX T7 Capped Message Transcription Kit is a symmetrical molecule , 100% of the caps in the RNA produced are in the correct orientation, increasing translation efficiency of the RNA.
Contents: A-Plus Poly(A) Polymerase, A-Plus 10X Reaction Buffer, 10 mM ATP, and Sterile RNase-Free Water.
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Rolling Circle transcription, durable transcripts, rNTPs

Kool NC-45 Universal RNA Polymerase Template
Kool NC-45 Universal RNA Polymerase Templates are small (28-150 nucleotide) circular single-stranded DNA (ssDNA) molecules. As observed in the laboratory of Dr. Eric Kool, these DNA nanocircles can be efficiently transcribed in vitro by a variety of DNA-dependent RNA polymerases (RNAP) by rolling circle mechanism. Bacterial RNAPs transcribe Kool templates in the absence of sigma factors, permitting studies of RNA polymerization mechanisms and inhibitor effects on the core RNAP. When using a Kool Template, RNA is produced in significant excess over the template DNA. Real-time detection methods for rolling circle transcription include use of dyes that exhibit fluorescence enhancement upon nucleic acid binding, such as SYBR Green I dye, SYBR Gold or RiboGreen (Molecular Probes), or use of molecular beacons. Screening for bacterial DNA-dependent RNAP activity. Screening compounds for RNAP inhibitor activity. Preparation of concatamers of short RNA sequences
In-vitro Transcription
Ribonucleoside-5-Triphosphate Solutions (RNTPs) 2-Florine-2-Deoxyribonucleoside-5Triphosphate Solutions

Cat. No. R109AT RA02825 R109CT RC02825 R109GT RG02825 R109UT RU02825 R344NT Product ATP 10 mM ATP 100 mM CTP 10 mM CTP 100 mM GTP 10 mM GTP 100 mM UTP 10 mM UTP 100 mM One Premixed Solution of all 4 NTPs (Premix in 1 tube contains ATP, CTP, GTP, and UTP) One Tube of each of 4 NTPs (ATP, CTP, GTP, UTP) 2-Fluorine-dCTP (2-F-dCTP) 50 mM 2-Fluorine-dUTP (2-F-dUTP) 50 mM Qty 5 moles 25 moles 5 moles 25 moles 5 moles 25 moles 5 moles 25 moles 5 moles each
Kool NC-45 RNAP Activity & Inhibitor Screening Kit

The Kool NC-45 RNAP Activity & Inhibitor Screening Kit utilizes the Kool NC-45 Template to screen inhibitors of rolling circle transcription by bacterial polymerases. Compounds can be screened for inhibition of E. coli RNAP or inhibitors can be assayed on an RNAP provided by the user. The high product-to-template ratio of rolling circle transcription allows a variety of detection methods in addition to following incorporation of radioactive nucleotides. End-point or real-time monitoring is possible with fluorescent dyes or with molecular beacons. The Kool NC45 Screening Kit uses SYBR Green I Dye (Molecular Probes) for real-time detection of RNAP activity. Benefits A rapid, simple and sensitive method to assay RNAPs. RNAP activity can be assayed without a promoter sequence, or knowledge of the promoter sequence. Very high product-to-template ratio. Provides a rapid and simple method for assaying RNAP activity in real time without post-processing. Easy to multiplex and automate for high-throughput screening of RNAP enzymes or RNAP inhibitors. Product Kool NC-45 Universal RNA Polymerase Template (1 pmole/l) Kool NC-45 RNAP Activity & Inhibitor Screening Kit Qty 100 l
RN02825 R2F110C R2F110U
25 moles each 1 mole 1 mole
DuraScribe T7 Transcription Kit D4uraScribe SP6 Transcription Kit

The DuraScribe T7 Transcription Kit* provides many benefits for making RNA for use in RNA interference (RNAi). The DuraScribe T7 Transcription Kit produces 2'-Fluorine-modified RNA transcripts-called DuraScript RNA-that are completely resistant to RNase A. DuraScript RNA is more stable in storage and in all RNA applications and can be made and used without the worry and time-consuming procedures normally required for keeping RNA transcripts intact. Recent research indicates that double-stranded DuraScript RNA can be delivered into cultured mammalian cells in the presence of serum and without the need for transfection reagents. Double-stranded oligonucleotides, linearized plasmids, or PCR products with a T7 promoter can be transcribed in a DuraScribe transcription reaction. The DuraScribe T7 RNA Polymerase, provided in the kit, is a mutant form of T7 RNA Polymerase that efficiently incorporates 2'-Fluorine-CTP (2'-F-CTP) and 2'-Fluorine-UTP (2'-F-UTP), as well as ATP and GTP into full length transcripts. Cat. No. DS010910 DS010925 DS041010 Product DuraScribe T7 Transcription Kit DuraScribe T7 Transcription Kit DuraScribe SP6 Transcription Kit Qty 10 rxns 25 rxns 10 rxns
Cat. No. KN411100
KNK49025
25 rxns
Tagetin RNA Polymerase Inhibitor

Tagetin RNA Polymerase Inhibitor is the only compound known to potently and selectively inhibit RNA polymerase III from a variety of eukaryotic organisms including mammalian cells, Saccharomyces cerevisiae, Drosophila melanogaster, Bombyx mori, and Xenopus laevis oocytes.It strongly inhibits Escherichia coli RNA polymerase and plant chloroplast RNA polymerase.Plant nuclear RNA polymerases I, II, and III are much less sensitive to Tagetin Inhibitor. Phage-encoded RNA polymerases such as SP6 and T7 are also relatively insensitive.With both eukaryotic and prokaryotic RNA polymerases, the degree of inhibition is template-dependent. Cat. No. T9705H T9701K T9702K Product Tagetin RNA Polymerase Inhibitor 20 U/l Tagetin RNA Polymerase Inhibitor Tagetin RNA Polymerase Inhibitor Qty 500 U 1000 U 2500 U
Eukaryotic RNA Polymerases

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Procedures & Products

MicroArray Analysis
MICROARRAY
Products available
RNA isolation
RNA Amplification
MicroArray Target Labeling
MicroArray Validation
MasterPure RNA Purification Kit ArrayPure NanoScale RNA Purification Kit MasterPure Yeast RNA Purification Kit mRNA-ONLY Eukaryotic mRNA Isolation Kit mRNA-ONLY Prokaryotic mRNA Isolation Kit
1-Round RNA Amplification TargetAmp 1Round Aminoallyl-aRNA Amplification Kit 101 TargetAmp 1Round Aminoallyl-aRNA Amplification Kit 102 TargetAmp 1Round aRNA Amplification Kit 103
AmpliScribe T7 Aminoallyl-RNA Transcription Kit Biotin-X-X-NHS
MonsterScript 1st-Strand cDNA Synthesis Kit TAQurate GREEN Real-Time PCR MasterMix TAQurate PROBES RealTime PCR MasterMixe FailSafe PROBES RealTime PCR System FailSafe GREEN Real-Time PCR System MasterAmp GREEN Real-Time RTPCR Kit
2-Round RNA Amplification TargetAmp 2Round Aminoallyl-aRNA Amplification Kit 1.0 TargetAmp 2Round aRNA Amplification Kit 2.0
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Selection Guide for TargetAmp aRNA Amplification Kits

TargetAmp 2-Round Aminoallyl-aRNA Amplification Kit 1.0 10 pg - 500 pg SuperScript III & SuperScript II 5 X 10 6 TargetAmp 2-Round aRNA Amplification Kit 2.0 10 pg - 500 pg SuperScript III & SuperScript II 5 X 10 6 TargetAmp TargetAmp 1-Round 1-Round Aminoallyl-aRNA Aminoallyl-aRNA Amplification Amplification Kit 102 Kit 101 25 ng - 500 ng SuperScript III 25 ng - 400 ng MonsterScript
MICROARRAY
Selection guide and Related Items
Name of Kit
TargetAmp 1-Round aRNA Amplification Kit 103 25 ng - 500 ng SuperScript III
Starting Total RNA Reverse Transcriptases Used Fold Amplification of mRNA Typical Yield of Amplified aRNA (HeLa cells) Total Time Required for Amplification In vitro Transcription Time Required End Product
5000
6000
5000
1.5 g - 70 g
1.5 g - 70 g
3 g - 60 g
4 g - 70 g
3 g - 60 g
2 Days
2 Days
1 Day
1 Day
1 Day
4 hrs & 9 hrs Aminoallyl Antisense RNA
4 hrs & 9 hrs
4 hrs Aminoallyl Antisense RNA
3 hrs Aminoallyl Antisense RNA
4 hrs
Antisense RNA
Antisense RNA
SuperScript III and SuperScript II reverse transcriptases (Invitrogen) are not included in the above TargetAmp Kits.
Kits for Isolation of mRNA mRNA-ONLY Prokaryotic mRNA Isolation Kit mRNA-ONLY Eukaryotic mRNA Isolation Kit
MasterPure RNA Purification Kit

ArrayPure Nano-Scale RNA Purification Kit

MasterPure Yeast RNA Purification Kit

Aminoallyl-UTP
Cat. No. AAU5202 Product Aminoallyl-UTP @ 50 mM Qty 2.5 moles
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TargetAmp Aminoallyl aRNA Amplification Kits

TargetAmp 1-Round Aminoallyl-aRNA Amplification Kit 101
TargetAmp 1-Round Aminoallyl-aRNA Amplification Kit 101 provides all reagents (except Reverse Transcriptase) to achieve at least 5000-fold amplification of polyadenylated mRNA present in total RNA. The kit uses an improved Eberwine-type linear amplification method to generate aminoallyl- containing antisense RNA (AA-aRNA), also referred to cRNA, corresponding to mRNA in the sample. The fold amplification is sufficient to obtain AA-aRNA for use as labeled target for microarray analysis from about 2500 or more cells (25 ng total RNA). The entire procedure takes about 2 hours of hands-on time and can be completed in one day, even with multiple samples. Produces microgram amounts aminoallyl-aRNA from 25 ng 500 ng total RNA. Greater than 5000-fold amplification. Optimized for use with SuperScript III Reverse Transcriptase (provided by the user). Simple 1-tube protocol can be completed in 1 day and requires only about 2 hours of hands-on time Multiple RNA samples can be easily amplified simultaneously, permitting high throughput. Reproducible with very low background Efficient protocols are available for labeling with Cy dyes, Alexa dyes, biotin, or other labels for any oligo or cDNA array format. TargetAmp kit 101 has been validated by the microarray group of a leading pharmaceutical company for aRNA yield, amplification efficiency, AA-aRNA size and quality, reproducibility of transcript detection when cDNA was made from the mRNA to be amplified using SuperScript III (Invitrogen) Reverse transcriptase. Product TargetAmp 1-Round Aminoallyl-aRNA Amplification Kit 101 TargetAmp 1-Round Aminoallyl-aRNA Amplification Kit 101 Qty 10 rxns
MICROARRAY
Amplification Kits
TargetAmp 1-Round aRNA Amplification Kit 103

This kit is similar to the TargetAmp 1-Round Aminoallyly-aRNA Kit 101 except that TargetAmp Kit 103 includes only the four canonical ribonucleotides, ATP, CTP, GTP and UTP. Using the nucleotides provided, unlabeled aRNA is produced using an improved 1-day Eberwine-type linear amplification process. This unlabelled aRNA will be amplified at least 5000 fold compared to the amount of the mRNA in the total RNA used in the reaction. Applications: Generate unlabeled aRNA or aRNA with your own label by amplification of polyadenylated mRNA in total RNA from small biological samples. Generate unlabeled aRNA for subsequent amplification by RT-PCR for other applications. Product TargetAmp 1-Round aRNA Amplification Kit 103 TargetAmp 1-Round aRNA Amplification Kit 103 Qty 10 rxns
Cat. No. TAU1R5110
TAU1R5124
24 rxns
TargetAmp 2-Round aRNA Amplification Kit 2.0

This kit provides all reagents (except Reverse Transcriptase) for generating antisense RNA by amplifying polyadenylated mRNA present in total RNA from biological samples. This is identical to TargetAmp 2-Round Aminoallyly Kit 1.0 except that it contains only the four canonical ribonucleotides ATP, CTP, GTP and UTP. The unlabelled RNA will be amplified 5-million-fold.Therefore it works on samples of minute size such as 1-50 cells. Cat. No. TAU2R5110 Product TargetAmp 2-Round aRNA Amplification Kit 2.0 TargetAmp 2-Round aRNA Amplification Kit 2.0 Qty 10 rxns
Cat. No. TAA1R4910
TAA1R4924
24 rxns
TAU2R51224
24 rxns
TargetAmp 2-Round Aminoallyl-aRNA Amplification Kit 1.0

TargetAmp 2-Round Aminoallyly Kit 1.0 reproducibly detects more expressed genes, including low-abundance transcripts, that can be detected using other RNA amplification kits. Applications: Amplification of ploy (A)- RNA from laser-captured cells, biopsy samples, cultured cells, or other minute cell samples for microarray studies. Benefits Produces microgram amounts of Aminoallyl-aRNA from 10 pg 500 pg total RNA. Greater than 5,000,000-fold amplification. Optimized for use with SuperScript III & SuperScript II Reverse Transcriptase (provided by the user). Detects low abundance transcripts
Biotin-X-X-NHS
Biotin-X-X-NHS is a useful reagent for labeling aliphatic amines for subsequent detection using avidin or streptavidin conjugates of fluorescent dyes or of enzymes that generate fluorescent or chemiluminescent products. The compound provides an easy and efficient reagent for labeling aminoallyl-derivatized RNA or aRNA obtained using various RNA amplification procedures, including that obtained using EPICENTREs TargetAmp Aminoallyl-aRNA Amplification Kits. Biotin-X-X-NHS can also be used to biotinylate DNA or RNA that contains nucleotides with aminoallyl- or other groups with reactive amines for use as nonradioactive probes for Northern, Southern, or other hybridization experiments. In addition to labeling, the addition of biotin to a biomolecule can provide a method for immobilizing or capturing the molecule using a streptavidin or avidin conjugate. Biotin-X-X-NHS can also be used to biotinylate reactive amines in proteins. A protocol for labeling aminoallyl-RNA is provided with the product.
Cat. No. TAA2R4910
Product TargetAmp 2-Round Aminoallyl-aRNA Amplification Kit 1.0 TargetAmp 2-Round Aminoallyl-aRNA Amplification Kit 1.0
Qty 10 rxns Cat. No. BXX51005 Product Biotin-X-X-NHS 2.5 mg/Vial Biotin-X-X-NHS 2.5 mg/Vial Qty 5 Vials 10 Vials
TAA2R4924
24 rxns BXX51010
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RNAi Libraries/ Transfection kits/Vectors

Mammalian RNAi shRNAmir libraries
shRNAmir triggers for mammalian RNAi are based on current knowledge of the endogenous microRNA processing pathway. shRNAmir constructs are designed to mimic a natural microRNA primary transcript, enabling specific processing by the endogenous RNAi pathway and producing more effective knockdown. In addition each gene-specific shRNAmir sequence has been selected based on thermodynamic criteria for optimal small RNA performance. The Expresion Arrest shRNAmir libraries incorporate several features aimed at increasing the efficiency and specificity of gene knockdown and provide solutions for diverse RNAi applications. Unique features include: microRNA-30 adapted design greatly increases knockdown specificity and efficiency Human and mouse genome-wide coverage Already cloned into retroviral and lentiviral vectors Molecular barcodes for multioplexed screening Transient, stable and in vivo options for RNAi Guaranteed knockdown
RNAi
shRNA Libraries, Vectors, Transfection kits
MSCV/LTRmiR30-PIG (LMP)
Non-Mammalian RNAi Drosophila RNAi library

The collection consists of dsDNAs covering 50% of the Drosophila genome. For each gene, 300-600 bases of exonic sequence was amplified by gene specific primers. The dmRNAi constructs contain dual T7 promoters, and are RNase free, making them ready for direct use in in vitro transcription.
Zebrafish Morpholinos
shRNA libraries
The RNAi consortium (TRC) is a collaborative effort based at the Broad Institute of MIT and Harvard. Over three years TRC will create lentiviral shRNA libraries targeting 15,000 human (TRC-Hs 1.0) and 15,000 mouse (TRC-Mm1.0) annotated genes with a goal of validating each shRNA in multiple cell lines. Currently the TRC lentiviral shRNA libraries consist of approximately 48,000 human shRNA constructs targeting 9700 human genes and 16,572 mouse shRNA constructs targeting 3300 mouse genes. Additional releases will occur every quarter.
C.elegans ORF-RNAi library

The C. elegans ORF-RNAi Library includes clones containing full open reading frames for over 11,000 genes, ready for your RNAi screens! These clones are derived from the C. elegans ORFeome Library v1.1. Full Coding Regions: The entire open reading frame is cloned into an RNAi feeding vector and compatible host. Feeding Ready: The cloned ORFs have been transformed into the RNAi feeding bacterial strain HT115(DE3)2 and the feeding vector, PL4440. RNAi Versatility: Plasmid preps of the ORF-RNAi clones can be used as templates for in vitro dsRNA synthesis for soaking or injection
RNAintro shRNAmir transfection kits

The RNAi ntro shRNA transfection kits combine the versatility and specificity of Expression Arrest shRNA mir, the simplicity of validated controls, and optimized transfection reagent- all in a guaranteed to work package. Each RNAi ntro kit includes: Two shRNAmir constructs- Your choice from the human and mouse genomes Arrest-In transfection reagent for shRNA delivery Positive controls- Luciferase or eGFP shRNA Negative control- Non silencing shRNA construct Transfection efficiency control- gal reporter
The C. elegans ORFeome clones that have been transferred into the RNAi feeding vector have been end sequence verified (Reboul 2003). A sampling of 100 RNAi clones have been randomly picked and sequenced.
Arrest-In transfection reagent

Arrest-In transfection reagent is a proprietary lipo-polymeric formulation, developed and optimized for transfection of shRNA plasmid DNA into the nucleus of cultured eukaryotic cells. Arrest-In transfection reagent also provides an enhanced uptake efficiency of the shRNA plasmid DNA into cells. Once in the cells Arrest-In promotes the entry of the shRNA containing plasmid into the nucleus where it is transcribed into a hairpin, enters the cytoplasm, is processed by the endogenous RNAi machinery into functional siRNAs.
MicroRNA-adapted Retroviral Vectors

A series of microRNA-adapted retroviral vectors have been recently developed in the laboratory of Scott Lowe at CSHL to enable the efficient expression of shRNAmir constructs from RNA Polymerase II (Pol II) promoters. These vectors produce highly efficient knockdown even when present at single copy in the genome. Since Pol II transcribes endogenous primary microRNA transcripts, the improved performance of these vectors seems to derive from natural mechanisms of RNA-dependent gene inhibition. Tetracycline-regulated retroviral vector- SIN-TREmiR30-PIG(TMP) MSCV-based self-inactivating (SIN)retroviral vector Express shRNAmir from tetracycline-regulated Pol II promoter (TRE-CMV) Regulated gene knockdown in tet-on or tet-off configurations. Select stableintegrants using Puromycin resistance GFP serves as amarker of shRNA expression
Expression Arrest shRNAmir controls

Changes in the mRNA or protein levels in cells treated with negative or non-silencing controls reflect non-specific responses in cells and can be used as a baseline against which specific knockdown can be measured.
Available Expression Arrest shRNAmir controls:

Non-silencing shRNAmir construct Exogenous positive controls eGFP shRNA construct Luciferase shRNA construct . -gal reporter
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Make shRNA/ DNA-Binding Proteins

MessageMuter shRNA Production Kit
RNA interference (RNAi) is a powerful technique for elucidation of gene function. Short hairpin RNA (shRNA) produced by chemical synthesis, in vitro transcription, or expressed in vivo , has been shown to silence genes as effectively as short dsRNAs. The MessageMuter shRNA Production Process The MessageMuter shRNA Production Kit utilizes a simple and unique 3step process (Figure 1) that yields transfection-ready shRNA in about 4 hours. A 17-base T7 Promoter Oligo is annealed to a DNA oligo designed by the user using detailed instructions provided. The ends of the annealed duplex are filled-in using the Exo-Minus Klenow to obtain a linear dsDNA with a T7 RNAP promoter at its 5'-end. Following in vitro transcription, the RNA produced spontaneously forms a hairpin structure (shRNA). Following clean-up, the shRNA is ready for transfection into cultured cells. The MessageMuter shRNA kit provides reagents to produce 10 shRNAs in sufficient quantities for hundreds of RNAi experiments. Applications Benefits Produces shRNA in vitro using one user-designed oligo and one in vitro transcription reaction. No need for annealing of sense and anti-sense RNA strands as required by other in vitro transcription-based methods . RC441MG Production of shRNA for RNA Interference, micro RNA studies, or other applications.
RNAi/MODIFYING ENZYMES
shRNA production / DNA-Binding Proteins
RecA Protein, E. coli

RecA Protein is a DNA-binding protein encoded by E.coli that plays integral roles in both homologous recombination and post-replicative DNA repair mechanisms. Initially, the protein binds preferentially to single-stranded DNA forming a nucleoprotein filament. The filament complex binds to naked duplex DNA and searches for regions of homology. Once a region of homology is found, strand displacement and exchange begins. Applications Site-directed mutagenesis through displacement loop structures. Targeted site-specific cleavage of small and large DNA. Enrichment of target sequences from libraries or other DNA pools. Product RecA Protein, E. coli (5 g/l) RecA Protein, E. coli (5 g/l) Qty 200 g 1 mg
Cat. No. RC44200
Single-Stranded DNA Binding Protein (SSB), E. coli

E. coli Single-Strand DNA Binding Protein (SSB) binds single-stranded DNA with high specificity. In vivo , the protein is involved in DNA replication, recombination, and repair. In vitro , SSB enhances several molecular biology applications by destabilizing DNA secondary structure and increasing the processivity of polymerases. Applications Transcription of ssDNA templates by MiniV RNAP. Targeting restriction endonuclease digestion to any RE site in cloned single-stranded DNA. Enhance the specificity and yield of PCR reactions. Improve DNA sequencing results through regions with strong secondary structure. Site-directed mutagenesis in conjunction with recA protein. DNA replication and recombination studies. Product Single-Stranded DNA Binding Protein (SSB) (2mg/ml) Qty 200 g
Cat. No. SSB02200
DNA Topoisomerase I, Vaccinia

Figure 1. Overview of the method used to produce shRNA using the MessageMuter shRNA Production Kit. All reagents are supplied except a 60- to 76-base DNA oligo designed by the user as specified in the kit. Topoisomerase I from vaccinia virus is a type I eukaryotic topoisomerase that removes both positive and negative superhelical turns (also called right- and left-handed supercoils) from covalently closed DNA. The product of the reaction is a covalently closed, circular DNA with fewer positive or negative superhelical turns. Applications Studying the effects of supercoiling on transcription in vitro . Studying chromatin reconstitution in vitro . Detecting mutant plasmids that differ in length by only one basepair. Product DNA Topoisomerase I, Vaccinia (10 U/l) DNA Topoisomerase I, Vaccinia (10 U/l) DNA Topoisomerase I, Vaccinia (10 U/l) Qty 500 U 1000 U 5000 U
Cat. No. MM031110
Product MessageMuter shRNAi Production Kit
Qty 10 rxns
Contents: T7 Promoter Oligo, 5X Annealing Buffer, Klenow exo- DNA Polymerase, dNTPs, 10X Fill-In Buffer, AmpliScribe T7- Flash , Enzyme Mix, AmpliScribe T7- Flash 10X Reaction Buffer, NTPs, DTT, Control luc21Oligo, RNase-Free Dnase, Sterile Water
Cat. No. VT710500 VT7101K VT7105K
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DNA Endonucleases & Exonucleases

DNA ENDONUCLEASES RNase-Free DNase I
RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I. It efficiently hydrolyzes double- and single-stranded DNA to a mixture of short oligo- and mononucleotides. Cat. No. D9902K D9905K D9910K Product RNase-Free DNase I (1U/l) RNase-Free DNase I (1U/l) RNase-Free DNase I (1U/l) Qty 2500 MBU 5000 MBU 10000 MBU Cat. No. EX4405K EX4425K Product
MODIFYING ENZYMES
Nucleases
DNA EXONUCLEASES Exonuclease III, E. coli

Exonuclease III digests duplex DNA in a 3' 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA. Because the rate of exonucleolytic excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and enzyme-to-DNA ratios, each template must be optimized using sample digestions to achieve the desired excision rate. Qty 5000 U 25000 U
Exonuclease III, E.coli (200 U/l) Exonuclease III, E.coli (200 U/l)
Lambda Terminase
Lambda Terminase is an endonuclease encoded by bacteriophage lambda that recognizes and cleaves DNA at cos sites, generating 5'-overhangs of 12 bases in length. A typical digestion requires only 1 U of Lambda Terminase for each microgram of DNA. Applications Generation of restriction maps of cosmid or fosmid clone inserts. Linearization of cos site-containing clones for in vitro packaging. Specific cleavage of chromosomal DNA for physical mapping. Cat. No. LT4450 LT44200 Product Lambda Terminase (2 U/l) Lambda Terminase (2 U/l) Qty X40520K 50 U 200U Exonuclease I, E. coli (20U/l) 20000 U
Exonuclease I, E. coli
Exonuclease I digests single-stranded DNA in a 3'5' direction. Although it requires the presence of magnesium and a free 3'-hydroxyl terminus for activity, it is active under a wide variety of buffer conditions and can be added directly into most reaction mixes. Exonuclease I can be heat-inactivated by incubation at 80C for 15 minutes.
Cat. No. X40501K X40505K
Product Exonuclease I, E. coli (20U/l) Exonuclease I, E. coli (20U/l)
Qty 1000 U 5000 U
Exonuclease VII
Exonuclease VII has high enzymatic specificity for single-stranded DNA and exhibits both 5' 3' and 3' 5' exonuclease activities. It is especially useful for rapid removal of single-stranded oligonucleotide primers from a completed PCR reaction when different primers are required for subsequent PCR reactions. Exonuclease VII digestion of singlestranded DNA occurs in the absence of magnesium.
*Includes 10X Reaction Buffer and 10 mM ATP
Endonuclease IV, E. coli

Endonuclease IV, cloned from the E. coli nfo gene, is a metalloenzyme that functions in vivo to repair free radical damage in DNA. The enzyme also has Class II abasic endonuclease activity, which has utility in many areas of DNA damage and repair research.Endonuclease IV is also useful in the study of the effects of anti-tumor drugs such as bleomycin on nucleic acids in vivo. Cat. No. E70100 Product Endonuclease IV, E. coli (2 U/l) Qty 100 U
Cat. No. EN510100 EN510250
Product Exonuclease VII (10 U/l) Exonuclease VII (10 U/l)
Qty 100 U 250 U
T4 Endonuclease V
T4 Endonuclease V has N-glycosylase and apurinic/apyrimidinic lyase (AP lyase) activities. Ultraviolet (UV) light produces covalent photoproducts in DNA, the most prevalent being a cis-syn cyclobutane pyrimidine dimer. T4 Endonuclease V locates and binds to pyrimidine dimers in doublestranded DNA. The enzyme then cleaves the N-glycosylic bond of the 5'pyrimidine of the dimer and breaks the phosphodiester bond 3' to the resulting abasic site. Cat. No. TE6605 TE661K TE665K Product T4 Endonuclease V (20 U/l) T4 Endonuclease V (20 U/l) T4 Endonuclease V (20 U/l) Qty 500 U 1000 U 5000 U
Lambda Exonuclease
Lambda Exonuclease is a highly processive 5' 3' exodeoxyribonuclease that selectively digests the phosphorylated strand of double-stranded DNA. The preferred substrate is blunt-ended, 5'-phosphorylated doublestranded-DNA. The enzyme has reduced activity against nicked DNA and against single-stranded DNA and gapped DNA. Cat. No. LE035H LE032K Product Lambda Exonuclease (10 U/l) Lambda Exonuclease (10 U/l) Qty 500 U 2500U
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DNA Exonucleases/RNA Endonucleases

RecBCD Nuclease, E. coli
RecBCD Nuclease is an exonuclease from E. coli that degrades single- and double-stranded DNA. Hydrolysis of the DNA is bi-directional from both the 3' and 5' ends and processive, producing nucleoside monophosphates. Magnesium is required for the exonuclease activity, while calcium, nickel, zinc, and copper inhibit exonuclease activity. Calcium allows doublestranded DNA unwinding (helicase activity) without hydrolysis. Applications Removal of contaminating bacterial chromosomal DNA in plasmid, fosmid, cosmid, and BAC clone or vector preparation. Cat. No. BCD0401K Product RecBCD Nuclease, E. coli Qty 1000 U Cat. No. Product
MODIFYING ENZYMES
Nucleases
RNA ENDONUCLEASES RNase A

Ribonuclease A (RNase A) is an endoribonuclease that cleaves singlestranded RNA at the 3'-end of pyrimidine residues, forming oligoribonucleotides having 3'-terminal pyrimidine-3'-phosphates. Pyrimidine-3'-monophosphates are also released by RNase A cleavage of adjacent pyrimidine nucleotides. Applications Removal of RNA from DNA preparations. Removal of unhybridized regions of RNA from DNA-RNA or RNA-RNA hybrids.
Qty 2 ml
Rec J Exonuclease
Rec J Exonuclease, derived from E. coli , catalyzes removal of deoxyribonucleoside monophosphates from single-stranded DNA in a 5' 3' direction. Its activity is dependent on Mg +2. Rec J Exonuclease can be heat-inactivated by incubation at 65C for 20 minutes. Applications Removes primers from completed PCR reactions. Degrades single-stranded linear DNA in dsDNA and plasmid preps.
MRNA092
Ribonuclease A (5 mg/ml)
Hybridase Thermostable RNase H

EPICENTREs patented Hybridase Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA. In contrast to E. coli RNase H, which is rapidly inactivated at 55C, Hybridase RNase H is active at high temperatures. It has optimal activity above 65C and can be used at temperatures up to 95C. The thermostability of the enzyme permits it to be used at temperatures that give the highest hybridization stringency for specific DNA:RNA heteroduplexes, maximizing sensitivity and selectivity while minimizing background due to nonspecific hybridization.
Cat. No. RJ411050 RJ411250
Product Rec J Exonuclease (10 U/l) Rec J Exonuclease (10 U/l)
Qty 50 U 250 U
Includes 10X Reaction Buffer.
Cat. No. H39500 H39100
Product Hybridase Thermostable RNase H Hybridase Thermostable RNase H
Qty 500 U 100 U
T5 Exonuclease
T5 Exonuclease is a highly efficient 5' 3' exonuclease for either singlestranded or duplex DNA. It has a tightly associated single-strand-specific endonuclease activity when used in the presence of 1-10 mM divalent magnesium ions. This activity may be selectively suppressed by using low concentrations of magnesium ions (< 1 mM), allowing nicked, double-stranded circular DNA to be gapped to a singlestranded circular species. The mode of action of T5 Exonuclease in vivo may be analogous to that of the 5' 3' exonuclease activity of E. coli DNA polymerase I. In the absence of divalent metal cofactors, T5 Exonuclease is able to bind to DNA with a single-strand arm adjacent to a duplex DNA region. Applications Plasmid mutagenesis methods. Oligonucleotide site-directed mutagenesis. Generation of plasmid-sequencing templates. Removal of denatured DNA from alkaline-based plasmid purification procedures for improved cloning procedures.
RNase H, E. coli
Ribonuclease H (RNase H) from E. coli is an endonuclease that specifically degrades the RNA in an RNA:DNA hybrid, without affecting DNA or unhybridized RNA. It will not degrade double-stranded DNA or singlestranded nucleic acids. EPICENTREs RNase H is highly purified and suitable for use in diagnostic probe research, as well as other applications. However, in contrast to Hybridase Thermostable RNase H, which can be used at temperatures up to 95C, E. coli RNase H is rapidly inactivated at 55C.
Cat. No. Cat. No. T5E4111K Product T5 Exonuclease (10 U/l) Qty 1000 U R0601K R0650H
Product Ribonuclease H, E. coli (10 U/l) Ribonuclease H, E. coli (10 U/l)
Qty 1000 U 5000 U
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RNA Endonucleases/ DNA & RNA Endonucleases

RNase III, E. coli
Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests double-stranded RNA (dsRNA) to dsRNA fragments that have 2-base, 3'-overhangs.Complete digestion of dsRNA results in dsRNA fragments of 12-15 bp. Cat. No. RN02950 Product RNase III, E. coli (1 U/l) Qty 50 U Cat. No. OC7810K Product
MODIFYING ENZYMES
Nucleases
DNA & RNA ENDONUCLEASES OmniCleave Endonuclease

OmniCleave Endonuclease is a highly purified enzyme from a recombinant E. coli strain that degrades single- and double-stranded DNA and RNA to di-, tri-, and tetranucleotides. Qty 10000 U 50000 U
OmniCleave Endonuclease (200 U/l) OmniCleave Endonuclease (200 U/l)
RNase T1, Aspergillus oryzae

Ribonuclease T1 (RNase T1) is an endoribonuclease that specifically cuts RNA or deaminated RNA at the 3'-end of guanosine residues and adjacent nucleotides through a 2', 3'-cyclic phosphate intermediate mechanism. EPICENTREs RNase T1 is cloned from Aspergillus oryzae and over expressed in E. coli to produce a highly pure enzyme without contaminating DNase or non-specific RNase activity. Cat. No. NT09100K NT09500K Product RNase T1, Aspergillus oryzae RNase T1, Aspergillus oryzae Qty 10,0000 U 50,0000 U
OC7850K
Mung Bean Nuclease

Mung Bean Nuclease is a single-strand-specific nuclease purified from sprouts of the mung bean Vigna radiata. Because Mung Bean Nuclease has higher specificity for single-stranded DNA and RNA than S1 Nuclease, it is the enzyme of choice for most applications requiring a single-strandspecific nuclease. Unlike S1 Nuclease, Mung Bean Nuclease will not cleave the intact strand of nicked duplex DNA. Because of these characteristics, it is preferable to S1 Nuclease for many applications. Cat. No. Product Mung Bean Nuclease (50U/l) Mung Bean Nuclease (50U/l) Qty 2000 U 5000 U
RNase I, E. coli
RNase I degrades single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates by cleaving between all dinucleotide pairs, unlike RNase A, which cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70C for 15 minutes, eliminating the requirement to remove the enzyme prior to many subsequent procedures.
M8202K M8205K
DNA & RNA EXONUCLEASES Terminator 5'-Phosphate-Dependent Exonuclease
Cat. No. N6901K N6905K
Product RNase I, E. coli (10 U/l) RNase I, E. coli (10 U/l)
Qty 1000 U 5000 U
Terminator 5'-Phosphate-Dependent Exonuclease (Terminator Exonuclease) is a 5'-to-3', processive exonuclease that degrades RNAs that have a 5'-monophosphate such as prokaryotic 16S and 23S rRNA and eukaryotic 18S and 28S rRNA. RNAs with a 5'-hydroxyl group, RNAs with a 5'-cap structure are resistant to degradation by Terminator Exonuclease. Thus, Terminator Exonuclease is ideal for producing an mRNAenriched sample from a total RNA preparation by selectively removing the large rRNAs. Terminator Exonuclease will also digest DNA with a 5'-monophosphate. Terminator Exonuclease is not inhibited by proteinaceous RNase inhibitors, such as RNasin (Promega Corp) or other placental ribonuclease inhibitors, or by Prime Inhibitor (Eppendorf). Applications Prepare mRNA-enriched samples from prokaryotic total RNA preparations in 1 hour without the use of resins or magnetic beads. Prepare mRNA-enriched samples from eukaryotic total RNA preparations in 1 hour without the use of Oligo(dT).
RiboShredder RNase Blend

RiboShredder is a cocktail of potent RNases that completely degrades unwanted RNA in DNA purification procedures. This highly active cocktail contains a proprietary optimized blend of non-mammalian RNase enzymes. Unlike other RNase cocktails, RiboShredder RNase Blend degrades all RNA, converting RNA to nucleoside monophosphates by cutting between all dinucleotide pairs. Applications Removal of RNA from genomic and cloned DNA preparations.
Benefits Completely degrades RNA rapidly. DNase-free. Cat. No. RS12100 RS12500 Product RiboShredder RNase Blend RiboShredder RNase Blend Qty 100 U 500 U
Cat. No. TER51020
Product Terminator 5-PhosphateDependent Exonuclease (1 U/l)
Qty
20 U
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Other Nucleases / Glycosylases / Excision Mixes

Other Nucleases from Sibenzyme
Cat. No. E323 E324 E341 E342 345 E346 Product Endonuclease I Endonuclease I Nuclease S1 Nuclease S1 Exonuclease III Exonuclease III Qty 4000 U 20,000 U 200 U 1000 U 4000 U 20,000 U
MODIFYING ENZYMES
Nucleases / Glycosylases
Base-Specific DNA Excision Mixes and DNA Glycosylases 8-Oxoguanine-DNA Excision Mix
EPICENTREs 8-Oxoguanine-DNA Excision Mix is a blend of enzymes that allows site-specific or random cleavage of DNA at oxidized guanine residues. The positions of the oxidized guanine residues in a DNA sequence can be mapped by sizing cleavage fragments on a sequencing-type gel from a fixed priming site, yielding data similar to a G-lane dideoxysequencing reaction.The first enzyme in the mix, 8-oxoguanine-DNA glycosylase depurinates oxidized G-residues. The second enzyme, T4 Endonuclease IV, then cleaves the deoxyribose phosphate backbone at apurinic sites, generating a polished 3'-hydroxyl end and releasing a DNA fragment with an abasic 5'-phosphorylated end. The minimum oligomer size that will serve as a substrate for excision by the 8-OxoguanineDNA Excision Mix is 6 base pairs. Applications Mapping of G residues in any DNA DNA repair studies
Nickase
Cat. No. E402 Enzyme N.Bst9 I - Nickase Recognition Sequence GAGTC(4/-) Qty 500 U
Other Nucleases from Jena Bioscience

Cat. No. EN-154L EN-154S EN-155L EN-155S EN-156L EN-156S EN-157L EN-157S EN-158L EN-158S EN-159 Product RNase T1 L Pack, (Recombinant, E. coli) RNase T1 S Pack, (Recombinant, E. coli) RNase T1 L Pack, (Recombinant, E. coli) RNase T1 S Pack, (Recombinant, E. coli) RNase TA L Pack, (Recombinant, E. coli) RNase TA S Pack, (Recombinant, E. coli) Exonuclease III L Pack (Recombinant, E. coli) Exonuclease III S Pack (Recombinant, E. coli) DNase Af L Pack (Recombinant, E. coli) DNase Af S Pack (Recombinant, E. coli) Exo/S1 Kit (ExoIII), (S1 Nuclease), Recombinant, E. coli) Qty 1,500,000 U 300,000 U 5 mg 1 mg 5000 U 1000 U 150,000 U 30,000 U 5 mg 1 mg 10,000/ 1000 U
Cat. No. OG51100
Product 8-Oxoguanine-DNA Excision Mix
Qty 100 rxns
Contents: 8-Oxoguanine-DNA Excision Enzyme Mix, Guanine Oxidation Reagent, and 10X 8-OxoG-DNA Excision Reaction Buffer.
Uracil-DNA Excision Mix

EPICENTREs Uracil-DNA Excision Mix is a blend of enzymes that cleave DNA at positions where uracil is present in place of thymine. The UracilDNA Excision Mix is useful for specific or random cleavage of DNA or for DNA repair studies, allowing mapping of uracil residues in any DNA. Uracil-DNA glycosylase in the Excision Mix removes uracil bases from DNA, creating a single base gap and leaving the deoxyribose phosphate backbone intact. Endonuclease IV in the Excision Mix then cleaves the DNA at each abasic site, leaving a 3-hydroxyl end and an abasic 5phosphorylated end. The minimum oligomer size that will serve as a substrate is 6 base pairs. Uracil-DNA Excision Mix digestion products can be analyzed by denaturing agarose gel electrophoresis or denaturing polyacrylamide gel electrophoresis. Applications Mapping of uracil-containing residues in any DNA. Mapping CpG islands. DNA repair studies. Product Uracil-DNA Excision Mix Qty 100 rxns
HK-UNG Thermolabile Uracil N-Glycosylase

HK-UNG is a Uracil N-Glycosylase that can be easily heat-inactivated. Uracil N-Glycosylase (also known as uracil-DNA glycosylase) hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. HK-UNG is active on both single- and double-stranded DNA that contains uracil, but has no activity on RNA or 2'-deoxyuridine-5'-monophosphate. HK-UNG is ideal for studying repair of abasic sites in double-stranded DNA. HK-UNG is fully active at 50C, but is inactivated by a 10minute incubation at 65C. Cat. No. HU59100 Product HK-UNG Thermolabile Uracil N-Glycosylase (1 U/l) HK-UNG Thermolabile Uracil N-Glycosylase (1 U/l) Qty
Cat. No. UEM04100
Glycosylase from Sibenzyme

Cat. No. E335 E336 Product Uracil-DNA Glycosylase(UDG) Uracil-DNA Glycosylase(UDG) Qty 200 U 1000 U
100 U
HU5901K
1000 U
Provided with Dilution Buffer
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Phosphatases
Tobacco Acid Pyrophosphatase
Tobacco Acid Pyrophosphatase (TAP) hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure, releasing the cap nucleoside and generating a 5'-phosphorylated terminus on the RNA molecule. Applications Preparation of templates for RACE (Rapid Amplification of cDNA Ends). 5' and 3'-end mapping of RNA. Ligation of oligoribonucleotides to TAP-treated cellular RNA for construction of full-length cDNA libraries. Mapping of transcription initiation sites for eukaryotic and prokaryotic transcripts. Radiolabeling of RNA for use in sequencing or as a hybridization probe. Product Tobacco Acid Pyrophosphatase (TAP) Tobacco Acid Pyrophosphatase (TAP) Tobacco Acid Pyrophosphatase (TAP) Tobacco Acid Pyrophosphatase (TAP) Qty 50 U 100 U 250 U 500 U Cat. No. H92025 H92050 H92100 Product
MODIFYING ENZYMES
Phosphatases
HK Thermolabile Phosphatase
Dephosphorylation of 5-protruding ends with HK Phosphatase prevents vector self-ligation and reduces the frequency of non-recombinant background transformants during molecular cloning. The enzyme then can be easily heat-killed prior to ligation reactions using the dephosphorylated vector so that residual phosphatase will not remove the 5-phosphate groups from DNA fragments to be cloned. However, HK Phosphatase is not active in dephosphorylating recessed 5-ends and has low activity on blunt ends. Further, the enzyme requires 5 mM divalent calcium cations, which are inhibitory to some restriction enzymes, so a separate 0.1 M CaCl 2 solution is provided for addition after the restriction endonuclease digestion. Applications Dephosphorylation of 5-phosphates from protruding ends of dsDNA for cloning or kinase end-labeling. Dephosphorylation of 5-phosphates from RNA for kinase end-labeling.
Cat. No. T19050 T19100 T19250 T19500
Qty 25 MBU 50 MBU 100 MBU
HK Thermolabile Phosphatase (1 U/l) HK Thermolabile Phosphatase (1 U/l) HK Thermolabile Phosphatase (1 U/l)
APex Heat-Labile Alkaline Phosphatase

APex Heat-Labile Alkaline Phosphatase is a new, recombinant enzyme preparation and is very pure and free from nuclease contamination. APex Phosphatase removes the 5'-phosphate from all types of DNA ends, including 5' protruding, blunt, and 5' recessed ends, and from RNA ends. The enzyme is irreversibly heat-inactivated by incubation at 70C for 5 minutes. APex Phosphatase can be added directly to most restriction enzyme (RE) buffers and is highly active over a broad range of temperature (up to 50C), pH (from 5-12), and salt conditions (e.g., up to 1 M Na+, NH 4+, K+, Cl - or Acetate -), and in the presence of 10% Triton X-100. To remove the 5'-phosphate from DNA, RNA or a nucleotide, simply incubate the substrate with APex Phosphatase at 37C for 10 minutes. Applications Dephosphorylation of DNA vectors prior to cloning to prevent recircularization. Preparation of 5'-nucleic acid termini for 5'-end labeling with polynucleotide kinase. Dephosphorylation of DNA/RNA substrates for other purposes.
Includes 10X TA Buffer and 0.1 M CaCl 2
NTPhos Thermolabile Phosphatase

NTPhos Thermolabile Phosphatase is a novel, recombinant enzyme that is highly active in removing phosphate groups from the 5'-position of ribonucleoside- or 2'-deoxyribonucleoside-5'-triphosphates, diphosphates or monophosphates. It can be used to remove 5'-phosphate groups from nucleotide substrates following reactions using enzymes such as RNAdependent or DNA-dependent RNA or DNA polymerases, terminal transferases or poly(A) polymerases in order to prevent the nucleotides from inhibiting, interfering with, or causing background problems in subsequent downstream reactions. Following removal of 5'-phosphates from the nucleotides, the NTPhos enzyme can be completely and irreversibly heat-inactivated by incubation at 65C for 15 minutes. Applications Removing 5'-phosphates from modified or unmodified NTPs, NDPs, NMPs, dNTPs, dNDPs, dNMPs, and inorganic pyrophosphate following in vitro reactions. Removing ATP from enzymatic reactions that use it as an energy source.
Cat. No. AP49010 Product APex Heat-Labile Alkaline Phosphatase (1 rxn/l) APex Heat-Labile Alkaline Phosphatase (1 rxn/l) APex Heat-Labile Alkaline Phosphatase (1 rxn/l) Qty 10 rxns
Cat. No. NT4905H
Product NTPhos Thermolabile Phosphatase (20 U/l) NTPhos Thermolabile Phosphatase (20 U/l) NTPhos Thermolabile Phosphatase (20 U/l)
Qty 500 U
AP49050
50 rxns NT4910K 100 rxns NT4920K
10000 U
AP49100
20000 U
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Kinases/Ligases
T4 Polynucleotide Kinase, Cloned
T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the gammaphosphate from ATP to the 5'-hydroxyl of single- and double-stranded DNA, RNA, and nucleoside 3'-monophosphates. The enzyme also removes the 3'-phosphate from 3'-phosphoryl polynucleotides, deoxyribonucleoside 3'-monophosphates, and deoxyribonucleoside 3', 5'-diphosphates to form a 3'-hydroxyl group. Cat. No. P0505H P0501K P0503K Product T4 Polynucleotide Kinase, Cloned (10 U/l) T4 Polynucleotide Kinase, Cloned (10 U/l) T4 Polynucleotide Kinase, Cloned (10 U/l) Qty 500 U 1500 U 3000 U
MODIFYING ENZYMES
Kinases/Ligases
CircLigase ssDNA Ligase

CircLigase ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i.e., circularization) of single-stranded DNA (ssDNA) templates having a 5'-phosphate and a 3'-hydroxyl group. Production of single-stranded DNA templates for rolling circle replication or rolling circle transcription experiments. Production of single-stranded DNA templates for RNA polymerase and RNA polymerase inhibitor assays. Cat. No. CL4111K CL4115K Product CircLigase ssDNA Ligase CircLigase ssDNA Ligase Qty 1000 U 5000 U
Includes 10X Reaction Buffer without ATP. ATP is available separately.
Ampligase Thermostable DNA Ligase

Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases. This exceptional thermostability permits extremely high hybridization stringency and ligation specificity. Ampligase DNA Ligase has no detectable activity in ligating bluntended DNA and has no activity on RNA or RNA:DNA hybrids. Ligation Amplification (Ligase Chain Reaction, LCR) which can distinguish between DNA sequences that differ by as little as a single base-pair and is a useful tool for detection of single nucleotide polymorphisms (SNPs). Repeat Expansion Detection (RED), a ligation-based method of genetic screening that detects DNA regions comprised of multiple nucleotide repeats. Simultaneous mutagenesis of multiple sites. Ampligase DNA Ligase can introduce single or multiple point mutations at specific sites by ordered ligation of PCR-amplified DNA fragments that have had point mutations introduced via mutant primers. Other ligation-based detection methods. Cat. No. A8101 A30201 A0102K A32250 A32750 A3202K A0110K A0125K A3210K A3225K A1905B A3201S Product Ampligase DNA Ligase Kit (5 U/l) * Ampligase DNA Ligase Kit (5 U/l) * Ampligase Enzyme and Buffer (100 U/l) Ampligase Enzyme and Buffer 5 U/l) Ampligase Enzyme and Buffer (5 U/l) Ampligase Enzyme and Buffer (5 U/l) Ampligase DNA Ligase (100U/l) Ampligase DNA Ligase (100U/l) Ampligase DNA Ligase (5 U/l) Ampligase DNA Ligase (5 U/l) Ampligase 10X Reaction Buffer Ampligase 1X Storage Buffer Qty 1000 U 5000 U 2500 U 250 U 750 U 2500 U 10000 U 25000 U 10000 U 25000 U 5 ml 1 ml
Contents: CircLigase ssDNA Ligase, CircLigase 10X Reaction Buffer, ATP, 50 mM MnCl2, CircLigase Linear ssDNA Control Substrate, Water
Fast-Link DNA Ligation Kit

Fast-Link DNA Ligation Kit uses a high-quality ligase, called Fast-Link DNA Ligase, that was cloned at EPICENTRE and then formulated to provide extremely rapid high-efficiency DNA ligation. Cohesive-end ligations can be performed in 5 minutes at room temperature. Cohesive-end ligations in 5 minutes at room temperature. Blunt-end ligations in 15 minutes at room temperature. Ligation of PCR product with A-overhangs in 1 hour at 16C. High ligation efficiency. Desalting of ligation products is not needed prior to transformation of competent cells. Product Fast-Link DNA Ligation Kit Fast-Link DNA Ligation Kit Fast-Link DNA Ligation Kit Qty 25 rxns. 50 rxns. 100 rxns.
Cat. No. LK11025 LK0750H LK6201H
Contents: Fast-Link DNA Ligase, Fast-Link 10X Ligation Buffer, 10 mM ATP
E. coli DNA Ligase

E. coli DNA Ligase is an NAD +-dependent enzyme that catalyzes the formation of phosphodiester bonds between complementary 3'-hydroxyl and 5'-phosphoryl termini of double-stranded DNA. The enzyme works best with cohesive dsDNA ends and is also active on nicked DNA. Blunt ends can be ligated in the presence of condensing reagents such as polyethylene glycol or Ficoll. Cat. No. DL04082H Product E. coli DNA Ligase (10U/l) Qty 200 U
T4 RNA Ligase
T4 RNA Ligase catalyzes the formation of a phosphodiester bond between a 5'-P-terminated nucleic acid donor and a 3'-OH nucleic acid acceptor in a template-independent manner. The enzyme is ATP-dependent, and is active RNA, DNA, as well as numerous nucleotide derivatives. Cat. No. LR5010 LR5025 Product T4 RNA Ligase (5 U/l) T4 RNA Ligase (5 U/l) T4 RNA Ligase (5 U/l) Qty 1000 U 2500 U 5000 U
*Contents : 1000/5000 U of Ampligase DNA Ligase, 10X Reaction buffer & control DNA.
LR5050
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Restriction Enzymes
Restriction Enzymes
Product Cat No. E287 E288 E267 E268 E001 E002 E289 E290 E003 E004 E005 E006 E163 E164 E179 E180 E007 E008 E011 E012 E211 E212 E013 E014 E213 E214 E173 E174 E015 E016 E017 E018 E019 E020 E235 E236 E159 E160 E221 E222 E117 E118 AspS9I Sau96I G^GNCC AspLEI HhaI GCG^C AsiSI SgfI GCGAT^CGC AsiAI AgeI A^CCGGT ApaI ApaI GGGCC^C Ama87I AvaI C^YCGRG AluI AluI AG^CT AhlI SpeI A^CTAGT AfeI Eco47III AGC^GCT AcsI ApoI RÂATTY AclWI BinI GGATC(4/5) AclI AclI AA^CGTT AccBSI BsrBI GAG^CGG AccB7I PflMI CCANNNN^NTGG AccB1I HgiCI G^GYRCC Acc113I ScaI AGTÂCT Acc65I (KpnI) G^GTACC Acc36I BspMI ACCTGC(4/8) Acc16I MstI TGC^GCA AauI Bsp1407I T^GTACA Enzyme AatII Prototype AatII Recognition Sequence GACGT^C Qty 500 U 2500 U 500 U 2500 U 200 U 1000 U 100 U 500 U 1000 U 5000 U 600 U 3000 U 500 U 2500 U 200 U 1000 U 1000 U 5000 U 200 U 1000 U 100 U 500 U 500 U 2500 U 200 U 1000 U 1000 U 5000 U 400 U 2000 U 1000 U 5000 U 1000 U 5000 U 100 U 500 U 200 U 1000 U 500 U 2500 U 1000 U 5000 U
RESTRICTION ENZYMES
Sibenzyme
Restriction Enzymes
Cat No. E257 E258 E231 E232 E063 E064 E021 E022 E023 E024 E025 E026 E027 E028 E029 E030 E457 E458 E149 E150 E033 E034 E205 E206 E219 E220 E035 E036 E253 E254 E147 E148 E037 E038 E039 E040 E181 E182 E263 E264 E285 E286 Bso31I Eco31I GGTCTC(1/5) BseX3I XmaIII C^GGCCG BsePI BsePI G^CGCGC Bse118I Cfr10I R^CCGGY Bse21I SauI CC^TNAGG Bse8I BsaBI GATNN^NNATC Bse3DI BsrDI GCAATC(2/0) Bse1I BsrI ACTGG(1/-1) Bsc4I BsiYI CCNNNNN^NNGG Bsa29I ClaI AT^CGAT Bpu14I AsuII TT^CGAA Bpu10I Bpu10I CC^TNAGC BmtI NheI GCTAG^C Bme18I AvaII G^GWCC BglII BglII A^GATCT BglI BglI GCCNNNN^NGGC Bbv12I HgiAI GWGCW^C BamHI BamHI G^GATCC AsuNHI NheI G^CTAGC AsuHPI HphI GGTGA(8/7) Product Enzyme AsuC2I Prototype CauII Recognition Sequence CC^SGG Qty 2000 U 10000 U 200 U 1000 U 500 U 2500 U 4000 U 20000 U 200 U 1000 U 1000 U 5000 U 1000 U 5000 U 1000 U 5000 U 1000 U 5000 U 200 U 1000 U 1000 U 5000 U 500 U 2500 U 500 U 2500 U 500 U 2500 U 200 U 1000 U 1000 U 5000 U 500 U 2500 U 200 U 1000 U 200 U 1000 U 200 U 1000 U 100 U 500 U
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Restriction Enzymes
Restriction Enzymes
Product Cat No. E291 E292 E185 E186 E245 E246 E273 E274 E261 E262 E207 E208 E239 E240 E043 E044 E051 E052 E265 E266 E093 E094 E259 E260 E237 E238 E305 E306 E227 E228 E083 E084 E103 E104 E119 E120 E031 E032 E283 E284 E171 E172 BstH2I HaeII RGCGC^Y BstFNI FnuDII CG^CG BstF5I (Fok) GGATG(2/0) BstENII MboI ^GATC BstENI EcoNI CCTNN^NNNAGG BstDSI DsaI C^CRYGG BstDEI DdeI C^TNAG BstC8I Cac8I GCN^NGC BstBAI BsaAI YAC^GTR BstAPI (ApaBI) GCANNNN^NTGC BstACI AcyI GR^CGYC Bst4CI Tsp4CI ACN^GT Bst2UI BstNI CC^WGG Bst2BI BsiI CTCGTG(-5/-1) Bst6I Ksp632I CTCTTC(1/4) BssT1I StyI C^CWWGG BssNAI SnaI GTA^TAC BssECI SecI C^CNNGG BspA2I AvrII C^CTAGG Bsp1720I EspI GC^TNAGC Enzyme BsoMAI Prototype BsmAI Recognition Sequence GTCTC(1/5) Qty 1000 U 5000 U 100 U 500 U 100 U 500 U 200 U 1000 U 1000 U 5000 U 1000 U 5000 U 200 U 1000 U 200 U 1000 U 1000 U 5000 U 200 U 1000 U 500 U 2500 U 200 U 1000 U 500 U 2500 U 200 U 1000 U 500 U 2500 U 1000 U 5000 U 200 U 1000 U 200 U 1000 U 500 U 2500 U 300 U 1500 U 500 U 2500 U Cat No. E143 E144 E077 E078 E151 E152 E071 E072 E251 E252 E299 E300 E307 E308 E197 E198 E065 E066 E303 E304 E297 E298 E229 E230 E053 E054 E277 E278 E203 E204 E055 E056 E309 E310 E241 E242 E057 E058 E059 E060 E243 E244 EgeI (NarI) EcoRV EcoRV EcoRI EcoRI DseDI DrdI DraIII DraIII DraI AhaIII CciNI NotI BtrI BtrI BsuRI HaeIII BstX2I XhoII BstV2I BbvII BstV1I BbvI BstSNI SnaBI BstSFI SfeI BstSCI ScrFI BstPAI PshAI BstNSI NspI BstMCI McrI BstKTI MboI BstHPI HpaI Enzyme BstHHI
RESTRICTION ENZYMES
Sibenzyme
Restriction Enzymes
Product Prototype HhaI Recognition Sequence GCG^C Qty 2000 U 10000 U GTTÂAC 500 U 2500 U GAT^C 200 U 1000 U CGRY^CG 500 U 2500 U RCATG^Y 200 U 1000 U GACNN^NNGTC 1000 U 5000 U ^CCNGG 100 U 500 U C^TRYAG 200 U 1000 U TAC^GTA 200 U 1000 U GCAGC(8/12) 100 U 500 U GAAGAC(2/6) 200 U 1000 U R^GATCY 500 U 2500 U GG^CC 1000 U 5000 U CACGTC(-3/-3) 50 U 250 U GC^GGCCGC 200 U 1000 U TTTÂAA 500 U 2500 U CACNNN^GTG 500 U 2500 U GACNNNN^NNGTC 300 U 1500 U GÂATTC 5000 U 25000 U GATÂTC 1000 U 5000 U GGC^GCC 200 U 1000 U
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Restriction Enzymes
Restriction Enzymes
Product Enzyme ErhI Prototype StyI Recognition Sequence C^CWWGG Qty 1000 U 5000 U FalI FalI (8/13)AAG(N) 5CTT(13/8) 100 U 500 U FatI NlaIII ^CATG 50 U 250 U FauI FauI CCCGC(N4/6) 50 U 250 U FauNDI NdeI CA^TATG 500 U 2500 U FblI AccI GT^MKAC 100 U 500 U FokI FokI GGATG(9/13) 100 U 500 U FriOI BanII GRGCY^C 1000 U 5000 U Fsp4HI Fnu4HI GC^NGC 100 U 500 U HaeIII HaeIII GG^CC 1000 U 5000 U HindII HindII GTY^RAC 1000 U 5000 U HindIII HindIII AÂGCTT 2000 U 10000 U HinfI HinfI GÂNTC 1000 U 5000 U HpaII HpaII C^CGG 500 U 2500 U HspAI (HhaI) G^CGC 1000 U 5000 U KpnI KpnI GGTAC^C 2000 U 10000 U Ksp22I BclI T^GATCA 1000 U 5000 U Kzo9I MboI ^GATC 200 U 1000 U MabI SexAI A^CCWGGT 200 U 1000 U MhlI SduI GDGCH^C 500 U 2500 U MluI MluI A^CGCGT 1000 U 5000 U
RESTRICTION ENZYMES
Sibenzyme
Restriction Enzymes
Product Enzyme Mly113I Prototype NarI Recognition Sequence GG^CGCC Qty 200 U 1000 U MroNI (NaeI) G^CCGGC 500 U 2500 U MroXI XmnI GAANN^NNTTC 200 U 1000 U MspI HpaII C^CGG 1000 U 5000 U Msp20I BalI TGG^CCA 100 U 500 U MspA1I NspBII CMG^CKG 500 U 2500 U MspR9I ScrFI CC^NGG 500 U 2500 U NruI NruI TCG^CGA 500 U 2500 U NruGI Eam1105I GACNNN^NNGTC 100 U 500 U PceI StuI AGG^CCT 1000 U 5000 U PciI BspLU11I A^CATGT 200 U 1000 U PctI BsmI GAATGC(1/-1) 500 U 2500 U Ple19I PvuI CGAT^CG 100 U 500 U PpsI PleI GAGTC(4/5) 25 U 100 U PsiI PsiI TTA^TAA 100 U 500 U Psp6I EcoRII ^CCWGG 100 U 500 U Psp124BI SacI GAGCT^C 1000 U 5000 U PspEI BstEII G^GTNACC 2000 U 10000 U PspLI SplI C^GTACG 300 U 1500 U PspN4I NlaIV GGN^NCC 1000 U 5000 U PspOMI (ApaI) G^GGCCC 1000 U 5000 U
Cat No. E061 E062 E153 E154 E155 E156 E209 E210 E009 E010 E271 E210 E247 E248 E157 E158 E095 E096 E067 E068 E201 E202 E073 E074 E075 E076 E161 E162 E069 E070 E079 E080 E081 E082 E187 E188 E121 E122 E049 E050 E085 E086
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Restriction Enzymes
Restriction Enzymes
Product Enzyme PspPPI Prototype PpuMI Recognition Sequence RG^GWCCY Qty 100 U 500 U PsrI PsrI (7/12)GAAC(N)6 TAC(12/7) 100 U 500 U PstI PstI CTGCA^G 4000 U 20000 U PvuII PvuII CAG^CTG 2000 U 10000 U RsaI RsaI GTÂC 1000 U 5000 U Rsr2I Rsr II CG^GWCCG 200 U 1000 U SalI SalI G^TCGAC 2000 U 10000 U SbfI Sse8387I CCTGCA^GG 200 U 1000 U SfaNI SfaNI GCATC(5/9) 100 U 500 U SfiI SfiI GGCCNNNN^NGGCC 500 U 2500 U Sfr274I XhoI C^TCGAG 2000 U 10000 U Sfr303I SacII CCGC^GG 1000 U 5000 U SmaI SmaI CCC^GGG 2000 U 10000 U SmiI SwaI ATTTÂAAT 1000 U 5000 U SmiMI MslI CAYNN^NNRTG 200 U 1000 U SphI SphI GCATG^C 200 U 1000 U Sse9I Tsp509I ÂATT 100 U 500 U SspI SspI AATÂTT 500 U 2500 U TaqI TaqI T^CGA 200 U 1000 U Tru9I MseI T^TAA 200 U 1000 U Tth111I Tth111I GACN^NNGTC 400 U 2000 U
RESTRICTION ENZYMES
Sibenzyme
Restriction Enzymes
Product Enzyme BstHHI Vha464I Prototype HhaI AflII Recognition Sequence GCG^C C^TTAAG Qty 2000 U 500 U 2500 U VneI ApaLI G^TGCAC 1000 U 5000 U VspI VspI AT^TAAT 1000 U 5000 U XbaI XbaI T^CTAGA 1000 U 5000 U XmaI XmaI C^CCGGG 100 U 500 U ZraI (AatII) GAC^GTC 200 U 1000 U Zsp2I NsiI ATGCA^T 1000 U 5000 U
Cat No. E143 E135 E136 E137 E138 E139 E140 E141 E142 E233 E234 E463 E464 E145 E146
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Restriction Enzymes
Restriction Enzymes
Cat. No. EN-101L EN-101S EN-102L EN-102S EN-103L EN-103S EN-104L EN-104S EN-105L EN-105S EN-106L EN-106S EN-107L EN-107S EN-108L EN-108S EN-109L EN-109S EN-110L EN-110S EN-111L EN-111S EN-112L EN-112S EN-113L EN-113S EN-114L EN-114S EN-115L EN-115S EN-116L EN-116S EN-117L EN-117S EN-118L EN-118S EN-119L EN-119S EN-120L EN-120S EN-121L EN-121S EN-122L EN-122S EN-123L EN-123S EN-124L EN-124S EN-160L EN-160S Product Alu I, L pack Alu I, S pack Asu II, L pack Asu II, S pack BamH I, L pack BamH I, S pack Bcl I, L pack Bcl I, S pack Bgl I, L pack Bgl I, S pack Bgl II, L pack Bgl II, S pack BseA I, L pack BseA I, S pack BseB I, L pack BseB I, S pack BseC I, L pack BseC I, S pack BshF I, L pack BshF I, S pack BsiS I, L pack BsiS I, S pack BssA I, L pack BssA I, S pack CspA I, L pack CspA I, S pack EcoR I, L pack EcoR I, S pack EcoR V, L pack EcoR V, S pack Hind III, L pack Hind III, S pack Hinf I, L pack Hinf I, S pack Hpa I, L pack Hpa I, S pack Kpn I, L pack Kpn I, S pack Mbo I, L pack Mbo I, S pack MspC I, L pack MspC I, S pack Nae I, L pack Nae I, S pack Nco I, L pack Nco I, S pack Not I, L pack Not I, S pack Dpn I, L-Pack Dpn I, S-Pack Qty 3000 U 600 U 17500 U 3500 U 50000 U 10000 U 12500 U 2500 U 10000 U 2000 U 6500 U 1300 U 3250 U 650 U 22500 U 4500 U 4000 U 800 U 35000 U 7000 U 11000 U 2200 U 1500 U 300 U 750 U 150 U 25000 U 5000 U 15000 U 3000 U 40000 U 8000 U 16500 U 3300 U 5000 U 1000 U 17500 U 3500 U 1500 U 300 U 6500 U 1300 U 1750 U 350 U 3000 U 600 U 1500 U 300 U 1250 U 250 U
RESTRICTION ENZYMES
Jena Bioscience
Restriction Enzymes
Cat. No. EN-125L EN-125S EN-126L EN-126S EN-127L EN-127S EN-128L EN-128S EN-129L EN-129S EN-130L EN-130S EN-131L EN-131S EN-132L EN-132S EN-133L EN-133S EN-134L EN-134S EN-135L EN-135S EN-136L EN-136S EN-137L EN-137S EN-138L EN-138S EN-139L EN-139S EN-140L EN-140S EN-141L EN-141S EN-142L EN-142S EN-143L EN-143S EN-144L EN-144S EN-145L EN-145S EN-146L EN-146S EN-170L EN-170S EN-171L EN-171S EN-150L EN-150S Product Nru I, L pack Nru I, S pack PspP I, L pack PspP I, S pack Pst I, L pack Pst I, S pack Pvu II, L pack Pvu II, S pack Rsa I, L pack Rsa I, S pack Sal I, L pack Sal I, S pack Sca I, L pack Sca I, S pack Sfi I, L pack Sfi I, S pack SgrB I, L pack SgrB I, S pack Sla I, L pack Sla I, S pack Sma I, L pack Sma I, S pack SnaB I, L pack SnaB I, S pack Sph I, L pack Sph I, S pack SseB I, L pack SseB I, S pack Ssp I, L pack Ssp I, S pack Sst I, L pack Sst I, S pack Sty I, L pack Sty I, S pack Taq I, L pack Taq I, S pack Xba I, L pack Xba I, S pack BstE II, L pack BstE II, S pack Acc I, L pack Acc I, S pack Nhe I, L pack Nhe I, S pack BspLU4 I, L Pack BspLU4 I, S Pack BspLU11 I, L Pack BspLU11 I, S Pack Sau3A I, L pack Sau3A I, S pack Qty 3500 U 700 U 4500 U 900 U 40000 U 8000 U 22500 U 4500 U 5000 U 1000 U 10000 U 2000 U 6000 U 1200 U 2750 U 550 U 8000 U 1600 U 25000 U 5000 U 5500 U 1100 U 1750 U 350 U 1250 U 250 U 10000 U 2000 U 3000 U 600 U 8000 U 1600 U 30000 U 6000 U 22500 U 4500 U 17500 U 3500 U 10000 U 2000 U 1500 U 300 U 2750 U 550 U 7500 U 1500 U 750 U 150 U 2500 U 500 U
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In Vitro Transposomics: Endless Possibilities

The following examples of In Vitro Transposomics applications are discussed in this category: EZ-Tn5<T7/KAN-2> Promoter Insertion Kit: Introduce a phage T7 promoter into cloned DNA and generate RNA transcripts in vitro or in vivo from the insertion site. EZ-Tn5 <R6K ori /KAN-2> Insertion Kit: Rapidly characterize plasmids that otherwise would not replicate in E.coli by randomly inserting an E.coli conditional origin of replication (R6K ori ) and a kanamycin resistance marker. EZ-Tn5 -Lactamase Fusion Kit: Directly select genes encoding cell envelope and secreted proteins by creating translational fusions to -lactamase. EZ-Tn5 pMOD Transposon Construction Vectors: Use these vectors to make a custom EZ-Tn5 Transposon having a selectable marker, reporter gene, control element, or any other DNA sequence of interest.
TRANSPOSOMICS
Insert Promoters, Origin of replication
Inserting a T7 RNA Polymerase Promoter to Transcribe from the Insertion Site EZ-Tn5 <T7/KAN-2> Promoter Insertion Kit
The EZ-Tn5 <T7/KAN-2> Promoter Insertion Kit provides an easy and reliable method to randomly insert a transposon containing a phage T7 RNA polymerase promoter into any DNA molecule in vitro . A simple two hour in vitro reaction randomly inserts a single EZ-Tn5 <T7/KAN-2> Transposon into the plasmid, cosmid, fosmid or BAC clone. Then, following transformation into E. coli , transposon insertion clones are selected on kanamycin plates. The insertion site of individual kanamycin-resistant insertion clones can be mapped or sequenced bidirectionally using the two sequencing primers included in the kit. The transposon end has no termination sequences, so RNA can be produced from chosen insertion clones by in vitro transcription from the T7 RNA polymerase promoter. Cat. No. Product EZ::TN <T7/KAN-2> Promoter Insertion Kit* EZ::TN Transposase EZ::TN <T7/KAN-2> Transposon KAN-2 FP-1 Forward Primer KAN-2 RP-1 Reverse Primer Qty
Criteria for Choosing a Transposon System

EPICENTRE has hyperactive Tn5-based EZ-Tn5 Transposon Systems and hyperactive phage Mu-based HyperMu Transposon Systems for both in vitro and in vivo applications. The properties of these and other commercial systems are summarized in the following table and important criteria for choosing between them are discussed below.
# Transposase Proteins Needed Artificial Transposon # Basepairs in a Transposon End Target Sequence Duplication (bp) Efficiency In vivo*
EZI03T7
10 rxns 10 U 1 pmole 1 nmole 1 nmole
TNP92110 TK0701 KFP9122 KRP0021
Transposition In vitro
Randomness
EZTn5 Hyper Mu** Tn7Type
High
High
Shown
ME, 19
1 (Tnp)
* Contents: EZ::TN Transposase, EZ::TN<T7/KAN-2> Transposon, EZ::TN 10X Reaction Buffer, EZ::TN 10X Stop Solution, Forward and Reverse Primer, Control Target DNA, Sterile Water
High
Medium
Shown
-51
1 (MuA)
Kit for Inserting Priming Sites and a MultiCopy-Inducible oriV
Medium
Not Detectable Not Done
Shown
Tn7L, 165 Tn7R, 90-198 4
3 (TsnABC)
EZ-Tn5 <oriV/KAN-2> Insertion Kit

The EZ-Tn5 < oriV /KAN-2> Insertion Kit is designed to simplify and speed up both DNA purification and sequencing of existing single-copy clones by integrating CopyControl capability. Use a short, one-step in vitro reaction to randomly insert a single EZ-Tn5 < oriV /KAN-2> Transposon containing an inducible, high-copy origin of replication (oriV) into the target DNA. Then, transform TransforMax EPI300 or EPI300T1 R Electrocompetent E. coli (sold separately) with an aliquot of the reaction and select for the kanamycin marker encoded by the EZ-Tn5 Transposon. One reaction will generate thousands of EZ-Tn5 <oriV/KAN-2> Transposon insertion clones. Each clone contains primer binding sites at the ends of the inserted transposon for sequencing. Amplify selected clones by adding the CopyControl Induction Solution. Cat. No. Product EZ::TN <oriV/KAN-2> Insertion Kit* EZ::TN Transposase EZ::TN <oriV/KAN-2> Transposon TN RP-1 Reverse Primer TN FP-1 Forward Primer Qty 10 rxns 10 U 1 pmole 1 nmole 1 nmole
Ty1
Low
Shown
Virus-Like Particle Complex
*The in vivo transposition frequency data here refers to the relative number of colonies obtained containing transposon insertions following electroporation of electrocompetent E. coli cells with a synaptic complex (Transposome Complex) formed between a transposon and the respective transposase. ** HyperMu MuA Transposase can insert transposons with Mu Ends having free 3'-termini into another DNA, but is unable to excise the transposon from another DNA.
EZ-Tn5 Systems have very high transposition efficiencies for in vitro insertion, as well as for in vivo insertion of an EZ-Tn5 Transposome Complex, the synaptic complex formed between an EZ-Tn5 Transposase and an EZ-Tn5 Transposon. Although EPICENTREs HyperMu MuA Transposase is at least 50 times more active for in vitro transposition than the MuA transposase from other suppliers and almost as active as the EZ-Tn5 System in vitro, the HyperMu Transposome Complex generates 10-100 times fewer insertions than the EZ-Tn5 System in vivo using E. coli.
EZI02VK TNP92110 VK0231 URP023 UFP024
*Contents: EZ::TN Transposase, EZ::TN < oriV /KAN-2> Transposon, EZ::TN 10X Reaction Buffer, EZ::TN 10X Stop Solution, Forward and Reverse Primer, Control Target DNA, Sterile water
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Plasmid Rescue, Insert Signal peptides for secreting proteins
TRANSPOSOMICS
Plasmid Rescue, Insert Signal Peptide
Inserting an E. coli Origin of Replication to Rescue Heterologous Episomes
Finding Sequences that Encode Proteins with Signal Peptides EZ-Tn5 -Lactamase Fusion Kit
The EZ-Tn5 -Lactamase Fusion Kit was developed for the direct selection of genes encoding cell envelope and secreted proteins. The kit features the EZ-Tn5 < blaM /R6K ori > Transposon which contains a -lactamase gene ( blaM ) that lacks both promoter and secretory signal sequences. A clone or library of clones is screened with a simple, one-step in vitro reaction that randomly inserts a single EZ-Tn5 < blaM /R6K ori > Transposon into the target DNA. E. coli cells are transformed with an aliquot of the reaction and plated on media containing ampicillin. Only insertion clones with translational fusions to genes encoding extracytoplasmic proteins will grow. These fusions generate hybrid proteins that can transport the blaM moiety through the inner membrane and confer resistance to ampicillin. Once Amp R clones are selected, use the primer binding sites at the ends of the EZ-Tn5<blaM /R6K ori> Transposon to map or bidirectionally sequence the insertion site with primers provided in the kit. Cat. No. EZI31BL TNP92110 BL0311 Product EZ::TN -Lactamase Fusion Kit** EZ::TN Transposase EZ::TN <blaM/R6K ori> Transposon TN FP-1 Forward Primer BLA RP-1 Reverse Primer Qty 10 rxns 10 U 1 pmole
EZ-Tn5 <R6K ori/KAN-2> Insertion Kit

The EZ-Tn5 <R6K ori/KAN-2> Insertion Kit facilitates sequencing and genetic analysis of plasmids or any other circularized DNA that would not otherwise replicate in E. coli. The kit uses the EZ-Tn5 <R6K ori/KAN-2> Transposon which carries the E. coli R6K conditional origin of replication (R6K ori) and a kanamycin resistance marker. A simple, one-step, 2-hour in vitro reaction is performed to randomly insert the EZ-Tn5 <R6K ori/KAN-2> Transposon into your target DNA. Then an aliquot of the reaction is used to transform an E. coli host expressing the pir gene product, which is required for replication from the R6K ori . Insertion clones are selected on kanamycin plates and can be sequenced bidirectionally using the provided primers that are homologous to the ends of the transposon. Applications Rescue plasmids or any other circularized DNA (e.g., mitochondria) that would not otherwise replicate in E. coli because they lack a recognizable origin of replication and/ or a selectable marker. Prepare DNA sequencing templates from transposon insertion clones and completely sequence the clone without primer walking or additional subcloning. Create a library of random gene knockouts in vitro to facilitate genetic analysis of plasmid-encoded genes. Product EZ::TN <R6K ori/KAN-2> Insertion Kit# EZ::TN Transposase EZ::TN <R6K ori/KAN-2> Transposon KAN-2 FP-1 Forward Primer R6KAN-2 RP-1 Reverse Primer Qty
Cat. No. EZI011RK
UFP024 BLA032
1 nmole 1 nmole
10 rxns 10 U 1 pmole
TNP92110 R6K0901
**Contents: EZ::TN Transposase, EZ::TN <blaM /R6K ori > Transposon, EZ::TN 10X Reaction Buffer, EZ::TN 10X Stop Solution Forward and Reverse Primers, Control Target DNA, Sterile Water
KFP9122 R6KRP091
1 nmole 1 nmole
EZ::TN Transposase
EZ::TN Transposase is a hyperactive form of Tn 5 transposase. The highly purified, single-subunit enzyme can be used to randomly insert any EZ::TN Transposon into any target DNA in vitro with an efficiency up to >10 6 insertion clones per standard reaction. When incubated with an EZ::TN Transposon in the absence of Mg 2+ , a stable EZ::TN Transposome complex is formed. The Transposome is so stable that it can be electroporated into living cells. Once in the cell, the Transposome is activated by intracellular Mg 2+ and the EZ::TN Transposon component is randomly inserted into the hosts genomic DNA.
#Contents: EZ::TN Transposase, EZ::TN <R6K ori /KAN-2> Transposon, EZ::TN 10X Reaction Buffer, EZ::TN 10X Stop Solution, Forward and Reverse Primers, Control Target DNA, Sterile Water
Kits for Inserting Priming Sites for Sequencing EZ-Tn5 <KAN-2>, EZ-Tn5 <TET-1>, and EZ-Tn5 <DHFR-1> Insertion Kits HyperMu <KAN-1> and HyperMu <CHL-1> Insertion Kits
Please view Page # 55
HyperMu MuA Transposase

HyperMu MuA Transposase is a hyperactive enzyme that is at least 50 times more active than the MuA transposase available from other suppliers of Mu-based sytems. The highly purified, single-subunit HyperMu Transposase can be used to randomly insert any HyperMu Transposon into any target DNA in vitro with an efficiency up to >106 insertion clones per standard reaction. Cat. No. TNP92110 Product EZ::TN Transposase (along with reaction and stop buffer) HyperMu MuA Transposase (along with reaction and stop buffer) Qty
10 U
THM03210
10 U
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Transposon Construction Vectors

Vectors for Making Custom EZ-Tn5 Transposons EZ-Tn5 Transposon Construction Vectors
EPICENTRE offers four different EZ-Tn5 pMOD Transposon Construction Vectors for the preparation of custom EZ-Tn5 Transposons (Table 1). Each vector contains a multiple cloning site (MCS) flanked by the hyperactive 19-bp Mosaic Ends (ME, denoted by < >) that are specifically and uniquely recognized by EZ-Tn5 Transposase. To prepare your transposon, clone any DNA sequence of interest (e.g., selectable marker, control element, reporter gene) into the MCS and then generate the transposon either by PCR amplification or restriction enzyme digestion. The Transposon Construction Vectors pMOD-2<MCS> and pMOD-3 <R6K ori/MCS> are pUC-based vectors. They consist of ME sequences that flank an MCS in a vector with a colE1 origin of replication (Figure 1). EZ-Tn5 pMOD-3<R6K ori /MCS> also contains an R6K ori within the ME sequences, which is useful for a variety of rescue cloning applications. These vectors work well for constructing transposons in most cases. The colE1 ori was eliminated in pMOD-4<MCS> and pMOD-5<R6K ori / MCS>, which only have the R6K ori (Figure 2). Replication from the R6K origin in these two new vectors is dependent on the pir gene product produced by TransforMAX EC100D pir + and pir -116 E.coli cells. Since most bacterial strains do not contain a pir gene, the uncut plasmid DNA that contaminates these transposon preparations cant replicate and background problems are eliminated. ori that is located ori that is located on vector outside of within the ME the ME sequences sequences colE1 colE1 R6K ori None None R6K ori None R6K ori
TRANSPOSOMICS
Custom Transposons
Figure 1. EZ-Tn5 Transposon Construction Vectors pMOD-2<MCS> and pMOD-3<R6K ori/MCS> replicate in standard E. coli strains using a colE1 origin of replication. Transposons made with the pMOD-3<R6K ori /MCS> vector also have an R6K ori within the transposon, for rescue cloning applications.
EZ-Tn5 Transposon Construction Vectors pMOD-2<MCS> pMOD-3<R6K ori /MCS> pMOD-4<MCS> pMOD-5<R6K ori /MCS>
Table 1 : Replication origins in EZ-Tn5Transpson Construction Vectors. Your custom EZ-Tn5 Transposon can be used for insertion into any target DNA in vitro . In vitro transposition of R6K ori -containing transposons can be used, for example, to rescue plasmids that would not otherwise replicate in E. coli because they lack a recognizable origin of replication and/or a selectable marker. You can also make your own EZ-Tn5 Transposomes for random insertion into genomic DNA of living cells by incubating your custom EZ-Tn5 Transposon with EZ-Tn5 Transposase in the absence of Mg 2+. The presence of an R6K ori enables you to rapidly rescue the genomic DNA flanking the transposon insertion site.
Cat. No. MOD0602
Product EZ::TN pMOD-2<MCS> Transposon Construction Vector EZ::TN pMOD-3<R6K ori/MCS> Transposon Construction Vector EZ-Tn5 pMOD-4<MCS> Transposon Construction Vector EZ-Tn5 pMOD-5<R6K ori/MCS> Transposon Construction Vector
Qty 20 g
Figure 2. EZ-Tn5 Transposon Construction Vectors pMOD-4<MCS> and pMOD-5<R6K ori /MCS> lack a colE1 origin of replication and require E. coli strains that produce a pir gene product for replication. Cat. No. MODFSP201 Product pMOD<MCS> Forward Sequencing Primer pMOD<MCS> Reverse Sequencing Primer pMOD<MCS> Forward PCR Primer pMOD<MCS> Reverse PCR Primer Qty
MOD1503
20 g
MOD4804
20 g
1 nmole
MOD4805
20 g
MODRSP202
1 nmole 1 nmole 1 nmole
Transposon Construction vectors include Forward and Reverse Primers
MODFP931 MODRP941
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In Vivo Transposomics: Revolutionizing Microbial Genetics

EZ-Tn5 Transposome Kits EZ-Tn5 <R6K ori/KAN-2>Tnp Transposome Kit
Among the advantages of transposon mutagenesis is that the transposon serves as a marker that can be used to clone and sequence the region of genomic DNA that has been disrupted. Nothing makes this cloning process easier than creating mutations in vivo with the EZ-Tn5 <R6K ori /KAN2>Tnp Transposome Kit. In addition to encoding a kanamycin-resistance gene, the transposon contains an E.coli conditional origin of replication (R6K ori ). The presence of this origin of replication enables you to propagate or rescue the region of genomic DNA into which the transposon has been inserted.
TRANSPOSOMICS
In-vivo mutagenesis
EZ-Tn5 <KAN-2>Tnp and EZ-Tn5 <DHFR-1> Tnp Transposome Kits (Contd.)

EZ::TN Transposome-mediated insertions have already been used successfully in a variety of bacteria including Acinetobactor, Campylobacter, Escherichia, Mycobacterium, Proteus, Pseudomonas, Saccharomyces, Salmonella, Trypanosoma, Xylella, and others. The number of transposition clones obtained is highly dependent on the transformation efficiency of the host cell. Cat. No. TSM99K2 Product EZ::TN <KAN-2>Tnp Transposome Kit* EZ::TN <DHFR-1>Tnp Transposome Kit* EZ::TN Transposase EZ::TN <KAN-2> Transposon KAN-2 FP-1 Forward Primer KAN-2 RP-1 Reverse Primer EZ::TN <DHFR-1> Transposon DHFR-1 FP-1 Forward Primer DHFR-1 RP-1 Reverse Primer Qty 10 rxns
TSM99D1
10 rxns
TNP92110 KAN2001 KFP9122 KRP0021 DFR1001 DFP9121 Figure 1. The process for rescue cloning of transposon insertion sites in genomic DNA using the EZ-Tn5 <R6K ori /KAN-2>Tnp Transposome and TransforMax EC100D pir + or TransforMax EC100D pir -116 Electrocompetent E. coli . DRP9131
10 U 1 pmole 1 nmole 1 nmole 1 pmole 1 nmole 1 nmole
*Each kit contains a ready-to-use Transposome complex with either a kanamycin resistance marker or dihydrofolate reductase gene (for selection with trimethoprim) and forward and reverse primers for sequencing.
Cat. No. TSM08KR
Product EZ::TN <R6K ori/KAN-2>Tnp Transposome Kit* EZ::TN Transposase EZ::TN <R6K ori/KAN-2> Transposon KAN-2 FP-1 Forward Primer R6KAN-2 RP-1 Reverse Primer
Qty 10 rxns
HyperMu Transposome Kits
HyperMu <R6Kori/KAN-1>Tnp Transposome Kit

The HyperMu <R6K?ori/KAN-1>Tnp Transposome is the stable complex between HyperMu Transposase and a HyperMu Transposon containing an R6K? E. coli conditional origin of replication and a kanamycin selectable marker. The Transposome can be electroporated into living cells where it is activated by intracellular Mg 2+ and the HyperMu Transposon is randomly inserted into the hosts genomic DNA. Transposon insertion clones are selected on kanamycin plates or by screening for a desired gene knockout or loss of gene function. The transposon insertion site can be sequenced directly using bacterial genomic DNA as template and the provided primers that are homologous to the ends of the inserted HyperMu Transposon. Alternatively, the insertion site and its flanking DNA can be rescued as a plasmid and then sequenced. Cat. No. MTS32RK Product HyperMu <R6K ori/KAN-1>Tnp Transposome Kit* HyperMu Transposase HyperMu <R6K ori/KAN-1> Transposon MUKAN-1 FP-1 Forward Primer MUKAN-1 RP-1 Reverse Primer Qty 10 rxns
TNP92110 R6K0901
10 U 1 pmole
KFP9122 R6KRP091
1 nmole 1 nmole
*Includes unlabeled forward and reverse sequencing primers.
EZ-Tn5 <KAN-2>Tnp and EZ-Tn5 <DHFR-1>Tnp Transposome Kits

EZ-Tn5 Transposome complexes are formed between an EZ-Tn5 Transposon and EZ-Tn5 Transposase and provide a simple and reliable method for generating a library of random gene knockouts in vivo . Just electroporate the EZ-Tn5 Transposome into any of a broad range of living cells and select for a marker encoded by the EZ-Tn5 Transposon. Because there is no need for cell conjugation, suicide vectors, or specific host factors, EZ-Tn5 Transposomes are ideal for creating mutants in species that have poorly described genetic systems or lack adequate molecular tools. Ready-to-use EZ-Tn5 Transposomes are available containing either a kanamycin selectable marker (<KAN-2>) or dihydrofolate reductase gene (<DHFR-1>) that can be selected on plates containing trimethoprim. These markers are readily expressed in many gram-negative bacteria. Or you can create your own EZ-Tn5 Transposome using one of the EZ-Tn5 pMOD Transposon Construction Vectors and EZ-Tn5 Transposase.
THM03210 MR6K323
10 U 1 pmole
MKFP321 MKRP322
1 nmole 1 nmole
**Includes unlabeled forward and reverse sequencing primers.
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Comp. Cells for R6Kori Clones/ Protein truncation with transposons

Competent Cells for Replication of R6K oriContaining Insertion Clones TransforMax EC100D pir+ and pir-116 Electrocompetent E. coli
The TransforMax EC100D pir + Electrocompetent E. coli and TransforMax EC100D pir -116 Electrocompetent E. coli each express the protein (the pir gene product) for replication of plasmids containing the R6K origin of replication (R6K ori ). The TransforMax EC100D pir+ Electrocompetent E. coli will maintain R6K ori -containing plasmids at approximately 15 copies per cell, for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir -116 Electrocompetent E. coli are for high copy propagation of up to 250 rescue plasmid copies per cell. Benefits lacZ M15 for blue/white screening of recombinants. Restriction minus ( mcrA , (mrr-hsdRMS-mcrBC )) enables efficient cloning of methylated DNA. Endonuclease minus ( endA1) to ensure high yields of DNA. Recombination minus ( recA1 ) for greater stability of large cloned inserts.
TRANSPOSOMICS
Comp cells/Protein Engg.
EZ-Tn5 Protein Truncation Kit Mu-End Protein Truncation Kit (Contd.)
Transformation Efficiency Greater than 1 X 10 9 colony forming units (cfu)/g with pR6Kan control DNA. Cat. No. ECP09500 Product TransforMax EC100D pir+ Electrocompetent E.coli TransforMax EC100D pir-116 Electrocompetent E.coli Qty 5 x 100 l
EC6P095H
5 x 100 l
Includes control vector containing an R6K ori.
Figure 1. The EZ-Tn5 Protein Truncation Kit can be used to create random, unidirectional deletions from the 5'-end (shown here) or 3'-end of a target sequence
Make Unidirectional Truncations of a Protein Encoded by Any Sequence EZ-Tn5 Protein Truncation Kit Mu-End Protein Truncation Kit
The EZ-Tn5 Protein Truncation Kit and the Mu-End Protein Truncation Kit provide a convenient method for generating a library of N-terminal and C-terminal protein deletions that can be expressed in E. coli . The EZ-Tn5 Protein Truncation Kit features the EZ-Tn5 <p15A ori /KAN-2/ T7Exp> Transposon, which is randomly inserted into any target DNA using a simple, in vitro reaction catalyzed by EZ-Tn5 Transposase. Then, the transposition reaction is amplified by PCR using one primer that anneals near a transposon end and another primer that anneals to a fixed point in the target sequence. Since the transposon is randomly inserted along the length of the target sequence, amplification generates a library of Nterminal or C-terminal deletions, depending on the choice of primers. Using the End-Repair Mix and Fast-Link DNA Ligase provided in the kit, the pool of PCR products is blunt-ended and self-ligated to create a library of kanamycin-resistant rescue clones that can replicate from the p15A ori in standard strains of E. coli . Rescue clones with an Nterminal deletion will also contain a transposon-derived T7-promoter region in a 5'-to-3' orientation. At least one-third of these clones will generate in-frame protein fusions that can be expressed in cells containing a T7 RNA polymerase gene (e.g., E. coli BL21).
Cat. No. EZI41110 HMI41110
Product EZ-Tn5 Protein Truncation Kit * Mu-End Protein Truncation Kit *
Qty 10 rxns 10 rxns
*Contents: EZ-Tn5 Transposase EZ-Tn5 <p15A ori /KAN-2 /T7Exp> Transposon, EZ-Tn5 10X Reaction Buffer, EZ-Tn5 10X Stop Solution, Control Target DNA, Sterile Water, End-Repair Enzyme Mix, End-Repair 10X Buffer, ATP, dNTPs, FastLink DNA Ligase, PCR Precipitation Solution, and P15 RP-1, P15 FP-1, KAN-2 RP-2, and KAN-2 FP-1 Primers. **Contents: HyperMu Transposase, Mu-End Transposon, HyperMu 10X Reaction Buffer, HyperMu 10X Stop Solution, Sterile Water, End-Repair Enzyme Mix, End-Repair 10X Buffer, ATP, dNTPs, Fast-Link DNA Ligase, PCR Precipitation Solution, and MU-1 RP-1 primer.
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F : 040-27016168
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Protein Engineering using Transposons

EZ::TN In-Frame Linker Insertion Kit
The EZ::TN In-Frame Linker Insertion Kit is a transposon-based protein modification system that was designed to rapidly and easily produce random 57-basepair (19 amino acid) insertions into a cloned DNA (e.g. genes and cDNA) that encodes an expressed protein. The kit features the EZ::TN < Not I/KAN-3> Transposon, which contains a kanamycin-resistance (Kan R) marker flanked by Not I restriction sites. An in vitro enzymatic reaction randomly inserts a single EZ::TN < Not I/ KAN-3> Transposon into each clone and produces >106 kanamycin-resistant insertion clones. Insertion clones for further analysis can be identified by gene functional analysis or by mapping or DNA sequencing of the transposon insertion site. Once clones are chosen, the kanamycin resistance gene is excised from the transposon by Not I digestion. Each Not Idigested clone is then ligated and transformed into high-efficiency E. coli. The resulting clones each contain a single, random 19-codon insertion that can be read to express protein in all three reading frames. Thus, the protein retains its original amino acid sequence on both sides of the insertion site. Benefits Generate a population of >10 6 insertion clones, each with a single randomly inserted transposon, to provide complete coverage of the clone. More versatile than traditional Linker Scanning Mutagenesisthe EZ::TN Transposon insertion is random and not limited to pre-existing restriction endonuclease sites in the cloned DNA. Unique Not I restriction site in the linker insertion facilitates mapping and additional gene construction. TNP92110 PDMSP01 EZ::TN Transposase pPDM FP-1 Forward Primer
TRANSPOSOMICS
Protein Engg.
Generate a complete population of random deletion clones without tedious and timed nuclease digests. Generate >10 5 independent deletion clones in a single, simple enzymatic reaction. The deletion process is highly random to ensure production of a complete population of deletion clones. Rapidly screen deletion clones by agarose gel size analysis. Product EZ::TN Plasmid-Based Deletion Machine** pPDM-1 Cloning Plasmid pPDM-2 Cloning Plasmid Qty
Cat. No. DPM9401
10 rxns 20 g 20 g 10 U 1 nmole
PDM1V941 PDM2V942
** Contents: EZ::TN Transposase, pPDM-1 and pPDM-2 Cloning Plasmids EZ::TN 10X Reaction Buffer, EZ::TN 10X Stop Solution, pPDM FP-1 Forward Primer, pPDM RP-1 Reverse Primer, Control pPDM-1 Clone, Sterile Water
pWEB-TNC Deletion Cosmid Transposition Kit

Please view page #
Cat. No. EZI04KN
Product EZ::TN In-Frame Linker Insertion Kit ## EZ::TN Transposase EZ::TN <Not I/KAN-3> Transposon Not I/KAN-3 FP-2 Forward Primer Not I/KAN-3 RP-2 Reverse Primer
Qty 10 rxns
Mutagenesis kits
JBS dNTP-Mutagenesis Kit is based on the incorporation of the mutagenic dNTP analogs 8-oxo-dGTP and dPTP into an amplified DNA fragment by PCR. The mutagenic dNTPs are eliminated by a second PCR step in the presence of the four natural dNTPs only, resulting in a rate of mutagenesis of up to 20%. JBS Error-Prone Kit introduces mutations in the gene of interest using modified PCR reaction conditions. An increased MgCl2 concentration, the addition of MnCl 2 and the unbalanced ratio of dNTPs induce an increased error-rate of the DNA-polymerase. The rate of mutagenesis achieved by error-prone PCR is in the range of 0.6-2.0%. JBS DNA-Shuffling Kit is designed to generate gene libraries by random fragmentation of one gene or a pool of related genes. A controlled fragmentation of the DNA is followed by a random reassembly in a selfpriming PCR reaction. This method allows the recombination of sequences from different, related genes. The overall rate of mutagenesis is approx. 0.7%. Cat. No. PP-101 Product JBS dNTP-Mutagenesis Kit*, (Random Mutagenesis by dNTP Analogs) JBS Error-Prone Kit**, (Random Mutagenesis by Error-Prone PCR) JBS DNA-Shuffling Kit***, (Random Mutagenesis by DNA Shuffling) Qty 15 Rxns
TNP92110 KN0801
10 U 1 pmole
NKFP072
1 nmole
NKRP062
1 nmole
## Contents: EZ::TN Transposase, EZ::TN < Not I/KAN-3> Transposon, EZ::TN 10X Reaction Buffer, EZ::TN 10X Stop Solution, Forward and Reverse Primers, Control Target DNA, Sterile Water Two unlabeled primers are provided with the kit to facilitate sequencing or mapping of the EZ::TN Transposon insertion site.
Make Truncations of Protein-Coding Sequences Cloned in Deletion Vectors EZ-Tn5 Plasmid-Based Deletion Machine
The EZ-Tn5 Plasmid-Based Deletion Machine is used to generate a complete population of deletion clones from DNA cloned into one of the specially constructed plasmid deletion vectors (pPDM-1 or pPDM-2) that are supplied with the kit. Random, unidirectional deletions are generated via an intramolecular transposition reaction by incubating a pPDM plasmid clone with EZ-Tn5 Transposase in a 2-hour reaction. A single reaction produces >10 5 independent deletion clones for use in a variety of applications including DNA sequencing and protein functional
PP-102
15 Rxns
PP-103
15 Rxns
Contents: *Polymerase, 10x Mutagenesis buffer, dNTP mix, dPTP, 8-oxo-dGTP, PCR grade water ** Polymerase, 10x Reaction buffer, 10x Error-prone solution, dNTP error-prone mix,PCR grade water *** DNase I, 10x Digestion buffer, DNase stop solution, Polymerase, 10x Shuffling buffer, dNTP mix, PCR grade water
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Transposon based DNA Sequencing

Kits for Inserting Priming Sites for Sequencing EZ-Tn5 <KAN-2>, EZ-Tn5 <TET-1>, and EZ-Tn5 <DHFR-1> Insertion Kits
EZ-Tn5 Insertion Kits are designed to simplify and speed up complete sequencing of any cloned DNA >2 Kb, without primer walking or subcloning. Just use a simple, one-step in vitro reaction to randomly insert a single EZ-Tn5 Transposon containing a selectable marker either kanamycin resistance (<KAN>), tetracycline resistance (<TET>), or trimethoprim resistance encoded by the dihydrofolate reductase gene (<DHFR>) - into the clone. Then, transform E. coli cells with an aliquot of the reaction and select for the marker encoded by the EZ-Tn5 Transposon. Use the primers provided in the kits to sequence insertion clones bidirectionally from primer binding sites at the ends of the inserted transposon. Benefits FasterCompletely sequence plasmid, cosmid, fosmid, and BAC clones 10-times faster than by subcloning or primer walking. More Complete Sequence CoverageBased on the highly random hyperactive in vitro Tn 5 transposition system ensures that the primer binding sites are distributed throughout your clone, even into regions that are difficult to subclone or primer walk. A single reaction generates up to >10 6 insertion clones. Use a single set of sequencing primers (provided in the kits) to completely sequence your clone. Cat. No. EZI982K EZI921T EZI912D TNP92110 KAN2001 KFP9122 KRP0021 TET92101 TFP9331 TRP9341 DFR1001 DFP9121 DRP9131 Product
SEQUENCING
Transposon Insertion kits
Qty 10 rxns 10 rxns 10 rxns 10 U 1 pmole 1 nmole 1 nmole 1 pmole 1 nmole 1 nmole 1 pmole 1 nmole 1 nmole
EZ::TN <KAN-2> Insertion Kit* EZ::TN <TET-1> Insertion Kit* EZ::TN <DHFR-1> Insertion Kit* EZ::TN Transposase EZ::TN <KAN-2> Transposon KAN-2 FP-1 Forward Primer KAN-2 RP-1 Reverse Primer EZ::TN <TET-1> Transposon TET-1 FP-1 Forward Primer TET-1 RP-1 Reverse Primer EZ::TN <DHFR-1> Transposon DHFR-1 FP-1 Forward Primer DHFR-1 RP-1 Reverse Primer
*Contents of Each Kit: EZ::TN Transposase, EZ::TN Transposon, EZ::TN 10X Reaction Buffer EZ::TN 10X Stop Solution, Forward and Reverse Primers, Control Target DNA, Sterile Water
HyperMu <KAN-1> and HyperMu <CHL-1> Insertion Kits

Like the EZ-Tn5 Transposon Insertion Kits, the new HyperMu Insertion Kits simplify and speed up complete sequencing of any cloned DNA that is too large to sequence with a single set of sequencing reactions. EPICENTREs Mu-based transposition system uses a unique HyperMu MuA Transposase that retains the highly random insertion characteristics of MuA transposase but is at least 50 times more active in vitro than the MuA transposase from other suppliers. The higher activity of HyperMu Transposase results in much higher transposition efficiencies that are critical for obtaining a sufficient number of transposon insertions to completely sequence a clone, especially those with large inserts.
Cat. No. HMI032K HMI039C MKAN320 MKFP321 MKRP322 MCHL390 MCFP391 MCRP392 Figure 1. The process for generating DNA sequencing template using an EZ-Tn5 Insertion Kit. Select inserts on kanamycin, tetracycline, or trimethoprim.
Product HyperMu <KAN-1> Insertion Kit** HyperMu <CHL-1> Insertion Kit** HyperMu <KAN-1> Transposon MUKAN-1 FP-1 Forward Primer MUKAN-1 RP-1 Reverse Primer HyperMu <CHL-1> Transposon MUCHL-1 FP-1 Forward Primer MUCHL-1 RP-1 Reverse Primer
Qty 10 rxns 10 rxns 250 ng 1 nmole 1 nmole 250 ng 1 nmole 1 nmole
**Contents: HyperMu Transposase, HyperMu <KAN-1> or <CHL-1> Transposon HyperMu MuA 10X Reaction Buffer, HyperMu 10X Stop Solution Forward and Reverse Primers, Control Target DNA, Sterile Water
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F : 040-27016168
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DNA Sequencing- for Automated and Cycle Sequencing

DNA Sequencing Kits for Automated Sequencers SequiTherm EXCEL II DNA Sequencing Kits (for automated sequencers)
These SequiTherm EXCEL II DNA Sequencing Kits have been optimized for use with the following DNA Sequencers: LI-COR 4000 and 4200 series Sequencers, NEN Global IR DNA Sequencers, ALF DNA Sequencers and ABI PRISM DNA Sequencers using dye primer technology. The kits contain SequiTherm EXCEL II DNA Polymerase, which greatly improves signal intensities, allowing increased sensitivity. The SequiTherm EXCEL II DNA Sequencing Kits incorporate advances that enable sequencing of a broad range of templates including those containing very difficult-tosequence regions. Both cycle and high-temperature isothermal (noncycle) sequencing protocols are provided. Benefits Sequence templates containing hairpin structures, regions of high GC or AT content, areas of interstrand reannealing, or nucleotide repeats. Kits compatible with LI-COR , NEN, and ALF DNA sequencers (both dye-primer and internal label) and with ABI PRISM automated sequencers (dye-primer). Greater protocol flexibilityoptional isothermal protocol to sequence templates that are recalcitrant to cycle sequencing. Termination Mixes contain 7-deaza-dGTP to reduce band compression during gel electrophoresis.
SEQUENCING
Kits for automated & manual Seq.
SequiTherm EXCEL II DNA Sequencing Kit

The SequiTherm EXCEL II DNA Sequencing Kit is the kit we recommend for both cycle sequencing and high-temperature isothermal (non-cycle) sequencing with either labeled primers or internal label incorporation using 32P, 33P, or 35S radionuclides. The kit incorporates advances that enable sequencing of a broad range of templates including those that are problematic for most other DNA polymerases. The kit contains SequiTherm EXCEL II DNA Polymerase, which improves signal intensities allowing sequencing from low amounts of template. Cat. No. SEM79020 SEM79050 SEM79100 Product SequiTherm EXCEL II DNA Sequencing Kit SequiTherm EXCEL II DNA Sequencing Kit SequiTherm EXCEL II DNA Sequencing Kit Qty 20 rxns 50 rxns 100 rxns
Contents: SequiTherm EXCEL II DNA Polymerase, SequiTherm EXCEL II Sequencing Buffer, G, A, T, C Termination Mixes, G, A, T, C LongRead Termination Mixes, SequiTherm EXCEL II Prelabeling Mix, Control Template Control Primer, Stop/Gel Loading Buffer, T4 Polynucleotide Kinase10X T4 Polynucleotide Kinase Buffer, Sterile Deionized Water
SequiTherm Cycle Sequencing Kit

The SequiTherm Cycle Sequencing Kit continues to provide researchers with a simple, rapid, and reliable method for obtaining high-quality sequence data from a wide variety of DNA templates. However, the SequiTherm EXCEL II DNA Sequencing Kit offers greater flexibility, improved sensitivity, and timesavings compared with this original SequiTherm Kit.
Cat. No.
Product
Qty
For LI-COR or NEN Global IR2 Sequencers SE9101LC SequiTherm EXCEL II DNA Sequencing Kit - LC for 25-41 cm gels 100 rxns
For LI-COR or NEN Global IR2 Sequencers SE9202LC SequiTherm EXCEL II DNA Sequencing Kit - LC for 66 cm gels 100 rxns
Cat. No. S20100
Product SequiTherm Cycle Sequencing Kit
Qty 100 rxns
For ALF DNA Sequencers or ABI PRISM Sequencers (dye-primer) SE8301A SequiTherm EXCEL II Long-Read DNA Sequencing Kit - ALF 100 rxns
Contents: SequiTherm DNA Polymerase, 10X Sequencing Buffer G, A, T, C Termination Mixes, Control Template, Control Primer Stop/Gel Loading Buffer, T4 Polynucleotide Kinase 10X Polynucleotide Kinase Buffer, Sterile Deionized Water
Contents: SequiTherm EXCEL II DNA Polymerase, SequiTherm EXCEL II Sequencing Buffer, G, A, T, C Termination Mixes, Control Template, Stop/Gel Loading Buffer, Sterile Deionized Water
SequiTherm DNA Polymerase

SequiTherm Thermostable DNA Polymerase is an enzyme preparation developed and optimized for cycle sequencing. SequiTherm DNA Polymerase has optimal activity at temperatures above 65 oC and is stable up to 95oC. The high reaction temperatures that are possible with SequiTherm DNA Polymerase eliminate most problems due to nonspecific priming or the inability to read through secondary structure. Cat. No. S3305H Product SequiTherm DNA Polymerase SequiTherm DNA Polymerase Stop/Gel Loading Buffer (Xylene Cyanol and Bromophenol Blue Dyes) Qty 100 rxns 200 rxns 1.5 ml
Primers, Stop/Gel Loading Buffers

Cat. No. SF81015 Product Stop/Gel Loading Buffer, Basic Fuchsin and Bromophenol Blue Dyes Stop/Gel Loading Buffer, Basic Fuchsin M13/pUC Primer, Forward M13/pUC Primer, Reverse Qty 1.5 ml
SA85025 P5713F P5713R
2.5 ml 2 g 2 g
S3301K S6501S
For other common sequencing primers, please view page # 13
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Purification of Recombinant Protein

Bacterial Cell Lysis and Removal of Nucleic Acids from Cell Lysates EasyLyse Bacterial Protein Extraction Solution
The EasyLyse Bacterial Protein Extraction Solution is designed for lysing bacterial cells for the isolation of proteins, especially recombinant gene products expressed in E. coli , without significant loss of enzymatic activity. It contains a highly active enzyme for cell lysis and a potent nuclease that reduces extract viscosity by digesting all nucleic acids in the sample. The EasyLyse Solution is formulated as a homogeneous reagent for ease of use in high-throughput applications. Just add the Enzyme Mix in Lysis Buffer to the cells for rapid lysis and removal of viscosity.
PROTEIN PURIFICATION
Bacterial lysis
Kit for Bacterial Lysis and Screening of Total Cellular Protein ReadyPreps Protein Preparation Kit (for total cellular proteins)
The ReadyPreps Protein Preparation Kit is a rapid, gentle, enzymatic method for lysing bacterial cells and reducing viscosity for the extraction of proteins, especially recombinant gene products expressed in Escherichia coli. The kit employs Ready-Lyse Lysozyme Solution, a stable non-avian, nonmammalian lysozyme that has a higher specific activity than egg white lysozyme. The higher specific activity of Ready-Lyse allows the addition of less Ready-Lyse Lysozyme, minimizing the amount of extraneous protein in the preparation. OmniCleave Endonuclease reduces the viscosity of cell lysates, facilitating handling and processing. RNase I is also provided for removing cellular RNA when the plasmid and protein are extracted simultaneously. The nucleases are included separately and can be added as needed. Applications Rapid screening of large numbers of expression clones for desired protein activity for testing the effects of various parameters such as culture conditions and induction protocols on the yield of active protein. Simultaneous isolation of plasmid and expression product from the same preparation. Preparative scale protein purification.
Cat. No. RP03750
Product EasyLyse Bacterial Protein Extraction Solution
Qty 500, 1-ml Purifications or48, 96-well Microplates, 100 l/well
Contents: Lysis Buffer, Enzyme Mix, MgCl 2 Solution
Ready-Lyse Lysozyme Solution for Protein Extraction

Ready-Lyse Lysozyme Solution is a non-mammalian, non-avian, recombinant lysozyme preparation for the lysis of Gram-negative (such as E. coli ) and Gram-positive (such as Bacillus sp. ) bacteria. The specific activity of Ready-Lyse Lysozyme is 200-fold higher than the specific activity of egg white lysozyme and, therefore, less is needed in a reaction. Also, unlike egg white lysozyme, Ready-Lyse Lysozyme Solution is stable at 20C, eliminating the need to prepare a fresh solution for each use.
Cat. No. RP78100
Product ReadyPreps Protein Preparation Kit (for total cellular proteins)
Qty 100 1-ml Preps
Reagents sufficient to process 5 g of cells. Bacteria lysed with Ready-Lyse Lysozyme Solution. Gram-negative Escherichia coli Salmonella typhimurium Actinobacillus pleuropneumoniae Rhodobacter sphaeroides Shewanella putrefaciens Flavobacteria odoratum Gram-positive Oerskovia xanthinolytica Bacillus subtilis Contents: ReadyPreps Lysis Buffer A, ReadyPreps Lysis Buffer B, OmniCleave Endonuclease, RNase I, 1 M MgCl 2, 0.5 M EDTA, and Lysis Test Reagent
Kit for Bacterial Lysis and Screening of Periplasmic Proteins PeriPreps Periplasting Kit (for periplasmic proteins)
The PeriPreps Periplasting Kit enables gentle, efficient solubilization of the outer membrane of E. coli cells, permitting rapid extraction of proteins from the periplasmic space. This can result in an enrichment of certain proteins compared with preparation by lysis of the entire cell. The PeriPreps Kit combines standard periplast preparation methods using lysozyme digestion and osmotic shock with the advantages of ReadyLyse Lysozyme. A spheroplast lysis buffer is also provided for isolation of spheroplast proteins. OmniCleave Endonuclease is included for digestion of nucleic acids, lowering the viscosity of the sample and improving the preparation of spheroplast proteins. Cat. No. PS81100 Product PeriPreps Periplasting Kit (for periplasmic proteins) Qty 100 Preps
Cat. No. RP03750 R1804M R1810M
Product Ready-Lyse Lysozyme Solution Ready-Lyse Lysozyme Solution Ready-Lyse Lysozyme Solution
Qty 2 X 106 U 4 X 106 U 10 X 106 U
OmniCleave Endonuclease
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Affinity Chromatography-Resins, syringe packed columns, MPLC columns

Affinity Chromatography Material
Immobilized nucleotides provide a convenient and rapid one-step purification procedure for a large number of proteins such as kinases, GTPases, chaperones, motor proteins, and others. Jena Bioscience offers affinity chromatography material that is tailor-made for purification (or for related assays) of particular NTP-binding proteins. NTPs and dNTPs that are linked to the matrix at various positions of sugar, base or phosphate moiety of the nucleotide different types and lengths of linkers types of chromatography material ranging from bulk media to pre-made columns that fit any machine
PROTEIN PURIFICATION
Affinity Chromatography
Immobilized -Aminohexyl-Nucleotides
(linked to the matrix via a C 6-spacer) -Aminohexyl-ATP-Sepharose -Aminohexyl-GTP-Sepharose -Aminohexyl-UTP-Sepharose -Aminohexyl-CTP-Sepharose -Aminohexyl-ITP-Sepharose -Aminohexyl-XTP-Sepharose -Aminohexyl-dATP-Sepharose -Aminohexyl-dGTP-Sepharose -Aminohexyl-dUTP-Sepharose -Aminohexyl-dCTP-Sepharose -Aminohexyl-dTTP-Sepharose
Different types of chromatography material

Bulk media
based on Sepharose 4B
Immobilized -Aminophenyl-Nucleotides
(linked to the matrix without a spacer) Aminophenyl-ATP-Sepharose (No linker)
Pre-packed syringe columns

based on Sepharose 4B
Immobilized 8-Aminohexyl-Nucleotides
(linked to the matrix via a C 6-spacer) 8-[(6-Amino)hexyl]- amino-ATP-Sepharose
Pre-packed screening columns

based on Sepharose 4B maximum amount 0.6 ml ready-to-use glass columns one each upper and lower frit upper and lower end piece special 96-well-MTP format column holder on request
Immobilized 8-Aminobutyl-Nucleotides
(linked to the matrix via a C 4-spacer) 8-[(4-Amino)butyl]- amino-ATP-Sepharose
Pre-packed MPLC columns

based on Toyopearl AF-650M 40 - 90 materials 1x Omnifit column kit 10x50 mm 1x adjustable end piece 2x female-female (1/4" to 6 mm) coupling 2 m PTFE tubing (1/16" OD x 0.8 mm ID)
Immobilized N6 -Aminohexyl-Nucleotides
(linked to the matrix via a C 6-spacer) N6-(6-Amino)hexyl-ATP-Sepharose
Immobilized N6-Aminobutyl-Nucleotides
(linked to the matrix via a C 4-spacer) N6-(4-Amino)butyl- ATP-Sepharose
Immobilized Nucleotides Immobilized -Aminophenyl-Nucleotides

-Aminophenyl-ATP (C 10 -Sephorose) (linked to the matrix via a C 10-spacer)
Immobilized 2,3-EDA-Nucleotides
2/3-EDA-ATP-Sepharose 2/3-EDA-GTP-Sepharose 2/3-EDA-UTP-Sepharose 2/3-EDA-CTP-Sepharose 2/3-EDA-ITP-Sepharose 2/3-EDA-XTP-Sepharose
Immobilized -Aminooctyl-Nucleotides
(linked to the matrix via a C 8-spacer) -Aminooctyl-ATP-Sepharose -Aminooctyl-GTP-Sepharose -Aminooctyl-UTP-Sepharose -Aminooctyl-CTP-Sepharose -Aminooctyl-ITP-Sepharose -Aminooctyl-XTP-Sepharose -Aminooctyl-dATP-Sepharose -Aminooctyl-dGTP-Sepharose -Aminooctyl-dUTP-Sepharose -Aminooctyl-dCTP-Sepharose -Aminooctyl-dTTP-Sepharose
Immobilized Tyrosines Immobilized Tyrosine (linked to the matrix via a C10 -spacer)
O-Phospho-L-Tyrosine-Sepharose (C 10 -linked)
Immobilized Tyrosine
(linked to the matrix without a spacer) O-Phospho-L-Tyrosine-Sepharose (no linker)
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Submarine Gel Electrophoresis

Owl Simple Cast Minigel Systems
The Simple Cast Horizontal Minigel Systems combine a proven, simple, in-place casting design with new improvements like lane visualization strips and fluorescent rulers. Three gel sizes are available for increased sample capacity and running length. The all-in-one design lets you cast and run gels in the same chamber, eliminating the need for additional equipment of any kind. Model B1A Gel Size 7 x 8cm Combs Included Model Two Combs: 6-Tooth & 10Tooth (1.5mm Thick) Two Combs: 10-Tooth & 14-Tooth (1.5mm Thick) Two Combs: 12-Tooth & 20-Tooth (1.5mm Thick) D3-14 Gel Size 23cm x 14cm
INSTRUMENTS
Electrophoresis
Owl Centipede Minigel System

The Liberal Wide Format Minigel Sytem offers a simple, convenient, and fast method for screening multiple samples on a single agarose gel. Up to 200 samples can be run simultaneously on one gel; producing clear, tight banding patterns with no smiling. Each side of the gel tray is fitted with a gasket, which allows the tray to fit snugly into the casting chamber to provide a leak proof seal without tape. Built-in leveling ensures the casting of flat, uniformly thick gels. Sample loading is greatly enhanced using microtiter format combs engineered to allow direct loading of samples from a 96-well plate using a multichannel pipettor.
Combs Included Four Combs: Four 50Tooth (1.5mm Thick) Microtiter Format)
B1
9 x 11 cm
B2
12 x 14cm
The Simple Cast systems unique pouring method withstands hot agarose (as high as 60oC without) warping the UVT gel tray. No tape, casting dams, or other accessories are required. All Simple Cast Systems accommodate two comb positions, allowing the user to run two series of samples simyltaneoulsy.
Owl Spider Electrophoresis System

The Spider Electrophoresis System allows for screening of up to 80 samples on a single agarose gel. Unit features durable acrylic construction with platinum electrodes and gold plated banana plugs to resist corrosion. The EasyCast TM Gasketed Gel Tray also makes casting easy with no tape, casting dams, no other accessories required. Complete System Includes: Buffer Chamber SuperSafe Lid With Attached Power Cords EasyCast TM Gasketed UVT Gel Tray 4 Combs: Two 30-Tooth & Two 40-Tooth, 1.5mm Thick (Other Combs Available) Casting Chamber Gel Size: 14.4cmW x 10.2cmL Running Buffer Volume: 600ml Each kit comes complete with 2 open frames, 2 solid back plates, 8 side clips, and sheets of pre-cut cellophane.
The Liberal Wide Format Minigel Sytem offers a simple, convenient, and fast method for screening multiple samples on a single agarose gel. Up to 200 samples can be run simultaneously on one gel; producing clear, tight banding patterns with no smiling. Each side of the gel tray is fitted with a gasket, which allows the tray to fit snugly into the casting chamber to provide a leak proof seal without tape. Built-in leveling ensures the casting of flat, uniformly thick gels. Sample loading is greatly enhanced using microtiter format combs engineered to allow direct loading of samples from a 96-well plate using a multichannel pipettor.
Owl WhirlSystem Recirculating Gel System

Model B3 Gel Size 12 cm x 14 cm Combs Included Two Combs: 12-Tooth & 20-Tooth (1.5 mm Thick)
The WhirlSystem prevents the formation of pH and ionic gradients by the patented WhirlSystem that allows self-recirculation of buffer without the need for a separate recirculator or accessories. Bubbles are collected on the cathode electrode and travel up a tube to the anode side of the gel box. The WhirlSystem is ideal for long runs, multiple sample sets, or RNA gels. It delivers clearer results for samples run over long time periods, and also eliminates uneven migration, banding distortion, or dissociation of pH-dependent glyoxicated RNA molecules that can result when ionic depletion occurs.
Owl Buffer Puffer Self Recirculating Gel Systems

The Buffer Puffer Self Recirculating Gel Systems feature a built-in buffer recirculation system for sharper bands. Bubbles are collected on the cathode electrode and travel up a tube to the anode side of the gel box. These moving bubbles create effective recirculation within the chamber with no external pumps, tubing, stir bars or additional assembly required. Systems are ideal for long runs, multiple sample sets, and RNA gels. Specifications : Gel Size 20cmW x 25cmL Combs Included 16,24 &36-Tooth (1.5mm thick)
Owl Gator Gel System

The Really Big Horizontal Gel System is designed for detailed RNA/DNA analysis. The unit includes the buffer chamber, SuperSafe lid with attached power cords, UVT gel tray with gasketed endgates, and 3 combs.
Model A2
Gel Size 20cm x 25 cm
Combs Included Three Combs: 16-Tooth, 24-Tooth & 36- Tooth (1.5mm Thick)
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Submarine & Vertical Electrophoresis Systems

Owl Millipede Horizontal System
Complete System Includes: Buffer Chamber SuperSafe Lid with Attached Power Cords UVT Gel Tray with Gasketed End Gates 4 Combs: (2) 25-Tooth, (2) 50Tooth, 1.5mm Thick Gel Size: 23cmW x 25cmL Warranty: 3 years The Millipede system allows researchers to screen up to 500 samples at one time. The new model A6 wide-format system offers a simple, convenient, and fast method for screening high numbers of samples on a single agarose gel. Up to 500 samples can be run simultaneously on one gel; producing clear, tight banding patterns with no smiling. Sample loading is greatly enhanced using microtiter format combs. Using these combs and a multichannel pipettor, samples may be loaded directly from a 96-well plate.
INSTRUMENTS
Electrophoresis
IBI Ultra-Wide Mini Gel-o-Submarine System

Gel Size: 23.5cmW x (7.5, 10, or 15) cmL Buffer chamber with leveling feet and bubble level Safety cover and power cords QuickCast gel casting system Two UVT gel trays, 7.5cm and 15cm long Two 1.5mm thick 25-well & 50-well microtiter combs
Designed to perform large numbers of samples, such as PCR or plasmid screening. Runs 96 samples from a microtiter plate in a single gel in under 90 minutes.
Owl Puffin Single Sided Minigel System
Owl Gator Horizontal Gel System

The Gator Horizontal Gel Systems are made to withstand heavy workloads and undertake different gel-running requirements. Comb slots on the UVT tray are positioned so that you can arrange two, three, or four sample sets to run equal lengths on the same gel.
Cat. No. MTP81
Description Puffin Complete System, 10 x 10cm Puffin Complete System, 10 x 10cm, With Cooling Core
MTP81-X
Model A1
Gel Size 13cm x 25cm
Combs Included Three Combs: 12-Tooth, 16-Tooth, 20-Tooth(1.5mm Thick) Four Combs: Two 25-Tooth & Two 50-Tooth(1.5mm Thick) Two Combs: Two 24-Tooth (1.5mm Thick)
A3-1
23cm x 40cm
A4
20cm x 25cm
Extended upper buffer chamber for even heat distribution. Designed to minimize buffer volume - less than 100ml per run. Full-length side clamps built-in for convenience. Spring loaded clamping to a silicone gasket ensures a leak proof seal. An optional built-in cooling chamber is available for faster runs with flat, even banding patterns Corrosion protected platinum electrodes and gold plated banana plugs.
Model A1 has the smallest format of the standard systems. This is a practical unit, which will conserve bench space. Model A3-1 is an extra long apparatus especially designed for screening large numbers of samples. The gel tray fits 25 and 50-tooth microtiter format combs for fast sample loading with a multichannel pipettor. Buffer exchange ports are standard on this model. Model A4 is a lower cost alternative, ideal for teaching labs. A wide selection of combs is available, including options that can double your sample capacity by doubling the number of sample wells. Each system is also available with built-in buffer exchange ports that allow you to circulate buffer for extended runs.
Owl Adjustable Vertical Systems

Model ADJ1, ADJ2 and ADJ3 are versatile and easy to use. The height of the upper buffer chamber can be lowered to run protein gels or raised to run sequencing gels. A soft silicone gasket ensures a leak proof seal. Black binder clamps or pony clamps are used with these units. Each system includes a removable lower and upper buffer chamber with attached pole rings, base with attached with pole rings, two heavy duty poles and safety interlocking lids with attached power cords.
IBI MP1015 Electrophoresis System

The ideal multi-purpose gel box for extended high resolution, linear runs and economical quick screens. Gel Size: 10cmW x 15cmL Buffer Volume: 500ml Safety cover and power cords Casting system and tray chamber Two 2.0mm thick x 16-tooth combs Leveling bubble and manual Cat. No. MTADJ1 MTADJ2 MTADJ3 Description 16cmW x 14-4cmL 20cmW x 20-70cmL 35cmW x 20-80cmL
Plates and spacers available in different sizes to match different heights.
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Vertical Electrophoresis Systems/Power Supplies

Owl Penguin Dual-Gel Water Cooled Electrophoresis Systems
Complete Package Includes: Upper Buffer Chamber with Internal Cooling Core, Lower Buffer Chamber, SuperSafe Lid with Attached Power Supply Leads, 4 Blank Glass Plates, 2 Notched Glass Plates, 2 Notched Alumina Plates, Blocking Plate for Single Gel Operation, Combs & Spacer Set (See Above), & Joey Gel Casting System. Complete System Includes: All of the components listed above except the Joey Gel Casting System.
INSTRUMENTS
Electrophoresis
IBI SH-500 & SH-3000 Power Supplies

These compact power supplies cover a wide range of requirements for electrophoresis separations. The SH-500 is ideal for DNA or RNA submarine gels, PAGE & SDS-PAGE, multiple horizontal gels and mini blotting applications. It features a built-in timer, constant voltage or constant current, and a unique Gel Saver mode (1mA output to prevent diffusion after the timer expires). The easy-to-use SH-3000 offers constant voltage, current and power, making it perfect for DNA sequencing systems and SDS-PAGE applications.
Model
Gel Size
Buffer Volume 250ml
Combs/Spacers Included* Two 15-Tooth, 0.8mmThick & 0.8mm Spacer Set Two 10-Tooth & Two 15-Tooth, 1.5mm Thick & 1.5mm Spacer Set Two 15-Tooth & Two 20-Tooth, 1.5mm Thick & 1.5mm Spacer Set
Model: Voltage: Max. Current: Power: Fault Detection: Operating Temperature: Dimensions (W x D x H): Weight:
SH-500
SH-3000
P8DS
10cmW x 8-10 cmL
P9DS
16cmW x 14-16 cmL
650ml
1-500V, 1V steps 10-3000V, 10Vsteps 300mA, 1mA steps 300mA, 1mA steps 0.3-150 watts 300 watts Stop/Audible Alarm Stop/Audiblealarm 0-40C 0-40C 17 x 24 x 7cm 27 x 28 x 11cm 1.6kg 4kg
P10DS
20cmW x 20 cmL
1250ml
Owl Lightning Volt Power Supplies

OSP-105

Labnet Power Station Power Supplies

The Power Station Power Supplies are ideal for DNA, RNA, and protein electrophoresis as well as blotting applications. All models feature a microprocessor that utilizes feedback loops to maintain tight control over operating parameters. A number of safety features are incorporated into the software including automatic crossover, no-load detection, sudden load change, and over voltage protection. Operation of the power supplies can be timed or continuous and the output can be set to either constant voltage or constant current. In the event of a power failure, the unit can be programmed to resume the run with the original settings upon restoration of power. An error code will be shown in the display to alert the user that a power failure has occurred. All operating parameters are set using the membrane keypad and displayed digitally. Four sets of output terminals allow for the simultaneous running of up to 4 gels from the same power supply. Model Voltage / Increments Current / Increments Timer Display 200 300 300 Plus
Upto 250V & 500mA Constant Voltage Ideal for DNA/RNA separations
OSP-300/OSP-500

Upto 300/500V & 500mA Constant Voltage & constant current Ideal for DNA/RNA separations 4 Output terminals 7-segment LED display Built-in timer Load sensing circuit
IBI Universal Protein Systems

These mini-format systems are designed to complete analysis 2 to 3 times faster than conventional gels and are ideal for running all types of precast gels. The Easy-Snap capturing system makes the placement of gels quick and easy. Both systems feature the patented IBI low-fog vented lid that reduces heat build-up. Model: Gel Size: Inner Buffer Tank Capacity: Outer Buffer Tank Capacity: Voltage Limit: Dimensions (W x D x H): Weight: IB90000 8 x 10cm 125ml 500ml 6,000 DC 17 x 17 x 18cm 2.2kg IB91000 10 x 10cm 150ml 350ml 6,000 DC 17 x 17 x 18cm 2.2kg
5-200V / 1V
10-300V / 1V
10-300V / 1V
.01-2A / .01A 0 to 999min 3 Digit LED
4-400mA / 1mA 0 to 999min 3 Digit LED 4, Parallel
4-500mA / 1mA 0 to 999min 3 Digit LED 4, Parallel
Outputs Terminals 4, Parallel
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F : 040-27016168
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Sequencing Gels / Electroblotters

Owl Otter Sequencing Gel Casting System
Cat. No. MTSGC-1 Product Otter System for gels up to 42cmW x 48cmL Otter System for gels up to 42cmW x 65.5cmL
INSTRUMENTS
Electrophoresis / Blotting
IBI Base Runner 100 & 200 Sequencers

System Components : Main unit assembly, upper & lower buffer reservoirs, one 0.4mm comb and spacer set (includes two 32-well conventional combs, one 32-well sharkstooth comb, one 48-tooth sharkstooth comb, and four side spacers), one set of power cords, instruction manual, and one (Base Runner 100) or two (Base Runner 200) 60cm thermocore plates.
MTSGC-2
The Otter uses an innovative method for casting in less than 3 minutes. Relying on a horizontal sliding plate procedure, capillary action draws gel between plates, and surface tension, not tape, keeps the gels from leaking. The system is fully adjustable to accomodate glass plates from 20cm to 42 cm wide. To cast a gel, simply pour acrylamide and slide top plate into position. Because the gel is poured horizontally, there is almost no mess; decreasing exposure to hazardous acrylamide.
Owl T-Rex Aluminum Backed Sequencing Systems

S3S and S4S Systems Include: Set of glass plates two blank, two notched Adhesive temperature indicator Top and bottom interlock safety lid with attached power cords 2 Sharktooth combs and spacer set (0.4mm thick) 2 Well Combs Cat. No. MTS3S MTS4S Description Complete Sequencing System, 35cmW x 45cmL Complete Sequencing System, 20cmW x 45cmL Gel Sizes: Plate Sizes: 27.3 x 19cm /41.2 x 19cm / 56.5 x 19cm 30 x 21.6cm /45 x 21.6cm / 60 x 21.6cm
Upper Buffer Tank Capacity: 500ml Lower Buffer Tank Capacity: 500ml Dimensions (W x D x H): 30 x 30 x 75cm
The unique, patented thermocore plates eliminate gel artifacts and minimize pre-run time. The glass-aluminum-glass laminate design distributes heat rapidly and efficiently, producing even sample migration for errorfree data interpretation. The buffer tank and tank drain assemblies are removable to permit safe and convenient disposal of radioactive and nonradioactive buffers, while interlocking safety covers on both the upper and lower tanks protect against electrical hazards. The Base Runner has the flexibility to accommodate 60, 45, or 30cm gels thanks to its unique adjustable height assembly, which is slanted to enhance gel visibility while holding the plates in place prior to positioning the buffer tanks.
Models S3S and S4S feature an anodized, floating aluminum plate heat sink that contacts the gel cassette and aids heat dissipation across the gel. Clear back on the upper buffer chamber allows easy visualization of wells. The upper buffer chamber is equipped with in-line stopcock drainage port. The lower buffer chamber is removable for easy cleaning. Acrylic screw clamps are mounted on the unit for easy assembly. Includes built-in leveling.
Owl Panther Semi-Dry Electroblotters

Complete System Includes:
IBI STS-45I Sequencer

Complete System Includes : Main assembly unit Aluminum thermoplate Two glass plates 0.4mm thick comb and spacer set One set of power cords Removable Lower Buffer Tank: Allows the user to dispose of buffer without moving the unit. Interlocking Safety Lids: Both lower and upper chambers feature a hinged, interlocking safety lid. Patented Slant-Back Design: With the slant-back design, the IBI STS45i sequencer assembles with ease. The design ensures that the glass plates remain in place during assembly. Built-In Aluminum Thermoplate: Distributes heat evenly, eliminating smiles or frowns. The unique epoxy-coated white background of the thermoplate makes for easy viewing of dye migration. 96-Well Capacity:The 96-well capacity accommodates 24 templates on a single gel, resolving up to 6,000 bases (6kb) of sequencing data per run. Easy-Turn Knobs: The easy-turn knobs create that perfect seal between the gel and the glass plate. These knobs prevent leaking and overtightening.
Base with built-in stainless steel cathode plate Lid with built-in platinum/ titanium anode Interlocking power cords 3 Heavy duty knobs (HEP1) 4 Heavy duty knobs (HEP-3)
The Panther offers rapid transfer of proteins or nucleic acid molecules from polyacrylamide or agarose gels to membranes. Traditional Southern, northern and western blotting methods can take from several hours to overnight; using the Panther you can complete the entire procedure in as little as one hour. Accurate and Efficient - Plate electrodes ensure complete, even transfer. Solid plate platinum/titanium and stainless steel electrodes are highly conductive and allow transfer at low voltages without external cooling systems. Plate electrodes also provide a uniform electric field for efficient, even transfers. Systems are graphite-free to avoid the decomposition associated with graphite electrodes. Two Panther semi-dry electroblotter systems are available. The HEP-1 system accommodates mini to full size gels with a transfer area of 20 x 20cm, designed specifically for the transfer from polyacrylamide or agarose gels. The HEP-3, with a transfer area of 35 x 45cm, is designed specifically for the transfer of nucleic acids from sequencing gels to nylon membranes.
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Electroblotters, Nitrocellulose, PVDF membranes, Blot papers

Owl Bandit Tank Electroblotting System
Complete System Includes: Blotting chamber with solid plate electrodes Safety interlocking lid with attached power cords Cooling modules 2 Blotting cassettes Blotting filter paper, 25 sheets, 18 x 20cm Transfer Area: 20cmW x 18.5cmL Buffer Volume: 3L
INSTRUMENTS
Electro blotting
S&S Westran Clear Signal & Westran S PVDF Membranes

Westran S is a 0.2mm hydrophobic PVDF membrane that gives superior protein binding capacity (200mg/cm2) and is designed specifically for sequencing applications. Offers the chemical resistance needed for Nterminal sequencing and provides high protein retention even after harsh wash steps. The smaller pore size allows for better retention of low molecular weight proteins. Westran S can also be used for non-sequencing Western blotting applications.
S&S Gel Blot Papers

Used for Wicking & Gel Support, Membrane & Gel support, Blotting.
The Bandit is designed to provide uniform, reproducible protein transfer over a wide molecular weight range. Solid plate electrodes consisting of platinum coated titanium anode and a stainless steel cathode provide the high current densities required for efficient and complete transfer. Novel use of alumina ceramic plate as part of its cooling module allows the system to effectively maintain low buffer temperatures during transfer.
Grade GB002 GB003 GB004
Thickness (Typical Values) Medium, Thick, Extra Thick, 1.2mm
Surface 0.4mm 0.8mm Rough
Absorbency SmoothMedium SmoothMedium Extra High
Owl Bandit MiniTank Electroblotter

Complete System Includes: Transfer Area: 9cmW x 9cmL Blotting Chamber With Platinum Wire Electrode Panels Safety Interlocking Lid With Attached Power Supply Leads 4 Color-Coded Blotting Cassettes With Foam Pads Blotting Filter Paper (16 Sheets, 9cmW x 9cmL)
S&S Protran Nitrocellulose Membranes

S&S Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions. And unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments. Prior to transfer, the membrane is simply wet in water and then in the transfer buffer. (1) Corners are notched for use with the Minifold I Dot-Blot System. (2) Cut to fit the Miniford II Slot-Blot System. Available in sheets, circles, rolls and microplate formats of different sizes and thickness (0.2 , 0.45 )
The Bandit MiniTank Electroblotter is designed to provide uniform, reproducible protein transfer over a wide molecular weight range with rapid, efficient transfers of up to 4 polyacrylamide mini protein gels simultaneously. Four color-coded cassettes are included with each system for easy setup. Cold water is recirculated through the cooling base of the unit, transferring heat from the buffer to keep it cool. The electrodes are deeply seated to prevent damage and are corrosion resistant.
S&S Minifold I Spot, Dot, and Slot-Blot Array Systems

Widely used for nucleic acid blots, hybridoma screening, TCA precipitations, and particle entrapment, the Minifold I system consists of four basic components: sample well plate, filter support plate, vacuum plenum, and metal clamping plate. The sample well plate is available in three configurations for producing spots, dots, or slots. All three plates are interchangeable and can be purchased as an accessory plate in conjunction with a complete system. Avialable in acrylic and Delrin. 96 Well Dot-Blot Plate (500l/well): Filter Area: 12.5mm2/dot 96 Well Spot-Blot Plate (200l/well): Filter Area: 2mm2/spot (1 x 2mm) 48 Well Slot-Blot Plate (1,000l/well): Filter Area: 6.24mm2/slot (7.8 x 0.8mm)
S&S Optitran Supported Nitrocellulose Membranes

Optitran supported membranes are nitrocellulose membranes with a polymer backing that provides exceptional strength and mechanical properties. They provide very low non-specific binding, without stringent washing and blocking conditions, using standard nitrocellulose protocols. Optitran membranes withstand repeated stripping and re-probing while providing excellent signal-to-noise ratio. (1) Corners are notched for use with the Minifold I Dot-Blot System. (2) Cut to fit the Miniford II Slot-Blot System. Available in sheets, circles, rolls and microplate formats of different sizes and thickness (0.2 m, 0.45 m)
S&S Minifold II 72 Well Slot-Blot Manifold

The Minifold II is a 72 well slot-blot manifold that is designed for precise quantitative solid phase assays and accepts all types of transfer membranes. The smaller slot dimensions require lesser samples than dotblotters and the smaller slot surface area results in higher signal intensities, allowing visualization of results in less time. Filter Area: Max. Capacity: Pressure: 6.0mm2/slot (8.0 x 0.75mm) 600 l/well, 72 wells 0.9 bar, vacuum
S & S Supercharge & Nytran N Nylon Membranes

Highest possible charge available Extremely low background binding. Consistent pore size & pore distribution Can be used with protocols standardized with earlier version nylon membranes
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Mini Centrifuges
Personal Benchtop Centrifuge MTI Mini Microcentrifuge I
For quick spin-downs from wall or caps of centrifuge tubes, microfiltration of HPLC samples, and cell separations. Angle rotor accommodates six 1.5/2.0ml tubes or six Ultra Spin Filter Systems. Adapters are available to hold 0.4 and 0.5 ml tubes. Starts and stops in seconds by opening and closing lid. There is an additional rotor which is designed to accommodate two 8 x 0.2ml strip tubes. Perfect for strip tube PCR applications. Max. speed: 6000 rpm, Max. RCF: 2000 x g
INSTRUMENTS
Centrifuges
The lower speed range of the Spectrafuge 7M Microcentrifuge makes it ideal for a variety of applications including quick spin downs, processing microfiltration devices, and basic cell separations. The fixed angle rotor accommodates 12 x 1.5/2.0ml tubes or smaller tubes through the use of adapters (comes supplied with 12 x 0.5ml/0.6ml adapters). For applications requiring below ambient temperatures, the unit may be used in a cold room.
Spectrafuge
Specifications: Maximum Speed14,000rpm, Maximum RCF: 16,000 x g Acceleration to Max. Speed: 10s Deceleration from Max. Speed: 9s Max. Radius for 1.5/2.0ml:7.3cm Timer: 1-30 min. or hold Noise Level: 55 dB(A)
MTI Mini Microcentrifuge II

The MTI6000 Mini Centrifuge is ideal for quick spin downs and microfiltration. Each unit comes supplied with a 6 x 1.5/2.0ml rotor with adapters for 0.5ml and 0.4ml tubes, and an interchangeable second rotor that will hold 28 x 0.2ml PCR strip tubes. Features an on/ off switch and a fixed maximum speed of 6,000rpm (2,000 x g).
Brushless motor: No maintenance, fast acceleration & deceleration. Cool Operation: Unique ducting system supplies continuous airflow to rotor chamber. Precise control: Variable speeds across broad range, suitable for applications such as microfiltration, separation of proteins and Nucleic acids. Complete package: Supplied with 18-place angle rotor for microtubes. Rotor accepts microtubes with screw caps and skirted bottoms also. Adapters available for smaller tubes.
Variable Speed Microcentrifuge

Model 8454 is a variable speed microcentrifuge with a maximum speed of 10,000 rpm at 5,600 x g. The space-saving design with Lexan polycarbonate cabinet makes it lightweight and easily moved from workstation to cold room, to refrigerator. Features microprocessor controlled 10 minute timer with audible time-up signal, momentary spin button, and automatic shutoff when lid is opened. Specifications Includes: - 6-Place Rotor for 1.5ml & 2.0ml Tubes - 12-Place Rotor for 0.5ml Tubes - 6 Adapters for 0.4ml Tubes
Spectrafuge 24D Digital Microcentrifuge

The new Spectrafuge 24D Digital Microcentrifuge combines innovations such as a unique, easy access rotor, an exclusive multi-flow air-cooling system and high performance drive with a digital microprocessor that precisely regulates all aspects of operation. Two smart knobs are used to control all operating parameters with speed and time values shown digitally on two large displays. Switching from rpm to gforce is accomplished by simply pressing the speed control knob. Run times from 30 seconds to 30 minutes (or continuous) are set with the timer knob. The powerful, brushless motor in the Spectrafuge 24D quickly and effortlessly accelerates the rotor to set speed. A computer designed isolation system allows for vibration free operation, even with a slight imbalance. A detector shuts down the centrifuge in the case of a significant imbalance. A specially designed air cooling system passes ambient air over both the top and bottom of the rotor as well as through the motor chamber of the Spectrafuge 24D. For samples that require below ambient temperatures, the Spectrafuge 24D may be used in a cold room.The unique design of the 24-place rotor allows easy access to the tops of the sample tubes. An optional StripSpin adapter snaps on top of the rotor for spinning 0.2ml tubes and strips. Specifications: Rotor Capacity 24 x 1.5/2.0ml Tubes Max. Speed 13,300rpm, Max. RCF 16,300 x g Timer 30 minutes, continuous, or momentary operation
Spectrafuge 7M Microcentrifuge
Rotor Capacity 12 x 1.5ml/2.0ml Tubes Max. Speed 10,000rpm, Max. RCF 7,176 x g Timer 60 minutes or continuous
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Liquid Nitrogen Dewars & Storage Devices

LD Series Dewars
The LD Series of cryogenic dewars are designed for storing and dispensing small amounts of liquid nitrogen. The series includes a beaker style dewar with a wide mouth (LD5) and pitcher-style model for easy pouring (LD4) Available in 4L, 5L, 10L, 25L, 35L and 50L capacity. Optional equipment includes Cap, Necktube core, Roller base, Tipping stand and liquid withdrawal device. Features: Modern construction and advanced insulation materials assure high thermal efficiency Ribbed high strength aluminum body, magniformed necktube design, low VOC Easy Operation - Snap-On cap and necktube assures tight closure and easy access Superior vacuum performance with super insulation provides maximum holding times.
INSTRUMENTS
Cryogenics
HC Series Refrigerators
Large storage capacity New rugged construction - magniformed necktube design, ribbed high strength aluminum body Versatile storage system - convenient canister index location ring and internal spider. Superior vacuum performance with super insulation provides maximum holding times. Security - Optional low-level alarm is available for use with more valuable samples Available in 12L, 20L, 34L and 35L capacity. Optional equipment includes Canisters, Necktube core, Roller base, Cryo sentry level alarm.
LS Series Refrigerators
The Taylor-Wharton Laboratory Systems (LS) are uniquely designed for large vial capacity in convenient box-type storage racks. They provide maximum holding times. This means lower operating costs per vial and fewer refills.
Taylor-Wharton XT Series Cryogenic Refrigerators

The Extended Time Series (XT) of cryogenic refrigerators is designed for long-term storage of a variety of materials at cryogenic temperatures. This series also offers a low profile model (XTL) for use in more confined workspaces. Available in 3L, 8L, 10L, 20L, 34L capacity. Optional equipment includes Canisters, Necktube core, Roller base and Cryo-Sentry alarm. Features Ribbed high strength aluminum body, magniformed necktube design Versatile Storage System - convenient canister index location ring and internal spider super vacuum performance Superior vacuum performance with super insulation provides maximum holding times Security - Accessory low-level alarm is available for more valuable samples Features LS6000 available with AutoTend Controller New Rugged Construction - ribbed high strength aluminum body, magniformed necktube design, and more durable paint Convenient Storage Systems - rack index location ring and internal location spider, computer compatible box storage is ideal for simple inventory management Super vacuum performance with super insulation provides maximum holding times Security - Accessory low-level alarm is available for more valuable samples Mobility - Roller bases are available
Unbreakable Stainless Steel Liquid Nitrogen Dewars
HC Series Refrigerators
The high capacity (HC) series of refrigerators are designed for storing large quantities of a variety of materials at cryogenic temperatures. Temperatures generally range between -320 F (-196 C) at the liquid surface, and -299 F (-184 C) under the closed necktube core. Safe and easy to use for the storage of liquid nitrogen and other low temperature liquid gases. Dewars are constructed of unbreakable stainless steel and are resistant to vibration, impact or shock. Dewars are supplied with lid and detachable carrying handle.
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F : 040-27016168
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Benchtop Freezers, Chillers, Freezer Storage Boxes & racks

Polar Block II -15C Personal Benchtop Freezer
Specifications Dimensions: 9.75D x 11.75W x 7.25H Net Weight: 10 lbs. Electrical: 220 VAC, 100 W Temperature Range: -15C (Typically) Performance: 35C to 39C Below Ambient Warranty: Two Years Parts & Labor
INSTRUMENTS
Cryogenics
ArcticChill Portable Cooler

Keeps samples cold for eight hours or longer. Small enough to fit in the palm of your hand, yet holds 12 x 1.5/ 2.0ml tubes! Patented refrigerant in sides, bottom, and lid surrounds samples in cold. Ideal for enzyme storage and transporting samples.
The Polar Block II is ideal for intermediate term storage of restriction enzymes and other labile reagents for applications requiring sub-freezing temperatures, such as ethanol precipitation. The Polar Block II uses a solid state, air-cooled thermoelectric device. It is equipped with a digital LCD temperature display and will reach and maintain temperatures from 35C to 39C below ambient (typically -15C) in approximately 30 minutes. Each unit comes with a removable machined aluminum block that accommodates 14 x 1.5ml microcentrifuge tubes. Additional blocks and block configurations are also available.
100 and 50 Well Freezer Storage Boxes

These sturdy, reusable polypropylene racks include a hinged lid and are rated to 80C. Racks will accommodate 1.5/2.0ml microtubes and standard 2.0ml cryo vials. The lids have raised edges to allow safe, non-slip storage of multiple racks.
MiniFridge II +4C to Ambient

The Minifridge II Cold Block Incubator is the convenient and reliable way to incubate or store samples at temperatures from +4C to ambient. The dry cold of the Minifridge II makes it the ideal substitute for messy ice buckets. The large LCD display and the microprocessor controller ensures easy and accurate temperature setting. The solid-state thermoelectric cooling module is compact and efficient. A large open well accommodates up to two standard dry blocks* (2 x 3 x 3.75) commonly used in dry block incubators. The unit can also accommodate flasks and beakers and can function as a small refrigerated water bath. Blocks not included Specifications Dimensions: 9.75D x 11.75 W x 7.25 H Weight: 10 lbs. Electrical: 220VAC, 100W Temperature Range: +4C to Ambient Performance: Typically 0.5 From Set Point Warranty: Two Years Parts & Labor
Both racks are alpha-numeric matrixed, hotstamped on the lid in contrasting color for fast, easy, and error-free identification of individual tube samples.
64 Well Freezer Storage Boxes

Accommodates 0.5, 1.5, and 2.0ml tubes. Autoclavable up to 15 mins. Freezer safe to -90C. Fits all standard freezer inventory systems. Fitted lids included. Contructed from polypropylene copolymer.
81 Well Freezer Storage Boxes
IsoFreeze Flipper Temperature Maintenance Racks

Available in two temperature ranges, these racks will provide consistent thermal protection for extended periods. Made of virtually unbreakable polycarbonate, both units are filled with a non-toxic gel, are stackable, and will accommodate up to 20 x 1.5/2.0ml micro or screw cap tubes, or 20 x 0.5ml tubes on the reverse, flip side. Available in , 0C & -20C
Replace your disposable cardboard racks with these sturdy, reusable polypropylene storage racks. Accommodates 0.5, 1.5, and 2.0ml tubes and withstand temperatures as low as -90C. Will easily fit standard freezer storage racks and drawers. An alpha-numeric grid is hot stamped on the unit in clear, easy to read contrasting colors. Bases are available in natural, black, or assorted fluorescent colors.
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Homogenizers Hand-held and Benchtop, Beadbeaters

PRO200 Hand-Held or Post-Mounted Laboratory Homogenizer
The Industrys Most Versatile & Affordable Lightweight/High-Torque Homogenizer. The PRO200 is fast and quiet, with most homogenizations taking just seconds at a maximum 72db at full speed. The PRO200 features a 125-watt, high-torque, variable speed motor and can be used with a variety of different PRO QUICK CONNECT rotor stator generators. The PRO200 can be used as a hand-held unit or can be postmounted, and will homogenize samples with volumes from 0.03ml to 2L. Variable Speed: 5,000 to 30,000rpm Power: 125 Watts Treatable Volume: 0.03ml to 2L
INSTRUMENTS
Homogenizing & Tissue Disintegration
PRO300 Benchtop Laboratory Homogenizer

Easily Handles All Common Laboratory Homogenizing Tasks The PRO300A & PRO300D feature a high-torque, variable speed motor that operates from 0 to 28,000rpm with a speed variation of just 1% over the entire speed range, regardless of changes in sample viscosity. The PRO300A features a 3/4 hp, 700-watt motor and the most advanced closedloop speed control available. The PRO300D offers the same advanced design as the PRO300A, but also has an LED display of generator or blade speed for optimum precision, accuracy and repeatability.
PRO QUICK CONNECT Rotor Stator Generators

They are manufactured from a high quality 300 Series stainless steel and can be completely sterilized by autoclaving, hot air, flaming or chemical processes. All generators offer the unique PRO QUICK CONNECT feature, which allows you to change generators with an easy quarter turn. The generators are also designed to help reduce the amount of air introduced into the sample during homogenizing to minimize foaming. Stainless steel sealed chamber assemblies are available for working with hazardous samples. Please call for more information. Flat Bottom Generators, Saw Tooth bottom Generators, Open Slotted Generators and Cryogenic Generators available in different sizes.
Variable Speed: 0 to 28,000rpm Power: 700 Watts Treatable Volume: 0.03ml to Several Liters Electrical: 220V
Mini-Bead Beater
The Mini-BeadBeater is a micro-sized cell homogenizer. A sealed chamber containing minute glass beads and 1 to 1.5ml of tissue or cell suspension is agitated violently for 1-3 minutes to achieve complete cell disruption. Even unusually tough samples such as bone or microbial spores are efficiently crushed by the colliding glass beads. The non-foaming method can be used to isolate delicate enzymes and organelles or to extract nucleic acids. Totally sealed, it is optimized for eukaryotic or bacterial disruption Vials: Polypropylene with O-ring seals and cap. Hold about 1ml of leadfree glass beads and 0.1 to 1.0ml of material to be disrupted. Vial snaps into spring-loaded holder. Motor: 0.62 Amp with 3 wire grounded cord. Zero to five minute automatically resetting timer.
Multi-Gen 7
The Multi-Gen 7 is a pack of 24 stainless steel generators for use with the PRO 200. Multi-Gen 7 features: 316 stainless steel and Teflon construction for homogenizing tough tissue samples and for repeated autoclaving Eliminates the need for generator cleaning between samples Hands-free ejection of generator Ideal for molecular applications, PCR, and microtube assays
PRO250 Hand-Held or Post-Mounted Laboratory Homogenizer

The PRO250 is a perfect fit between the PRO200 and the larger benchtop PRO homogenizers. Its light enough to be hand held for convenience, yet it has extra torque for processing difficult samples. Combining the power of a 3/4 hp, 576 watt motor with a smooth variable speed control (10,000 to 30,000 rpm), the PRO250 can handle samples from 0.05ml to several liters. Like all PRO homogenizers, the PRO250 accepts PRO QUICK CONNECT rotor stator generators for fast and virtually effortless changeover. PRO250 Series Specifications Power: 576 Watts; Electrical: 220V Single Handed Operation Stand Assembly with Easy-Lift mechanism Variable Speed: 10,000 to 50,000 rpm Treatable Volume: 0.05ml to Several Liters Sample Size: 0.02g to 100g
Tissue Tearor Mixer & Homogenizer

The Tissue-Tearor is a rotor-stator type tissue homogenizer, which can rapidly homogenize, disperse and emulsify samples in 0.5-50ml of liquid. During operation, the suspended material is drawn into the core of the homogenizer by a rotor turning at 30,000rpm. The material is repeatedly cycled through six narrow slits in the stator where it is rapidly sheared and disintegrated by high frequency mechanical action. Complete homogenization of tissues (muscle, liver, breast tissue, etc.) is usually achieved in ten to thirty seconds.
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Vortex Mixers and Stirrers

Bio-Pulverizer
The Bio-Pulverizer reduces tissue hard-frozen in liquid nitrogen to a fine powder. Called freeze-fracturing, this method is especially useful for tough, fibrous tissues such as skin, cartilage, cornea, etc. The Bio-Pulverizer consists of a hole machined in a stainless steel base, into which fits a special piston. In a typical procedure, up to 10g of animal or plant tissue is hard-frozen in liquid nitrogen and placed in the pre-chilled Bio-Pulverizer. The piston is mounted on an easy to use trigger mechanism that is squeezed to deliver a blow to the brittle tissue reducing it to a fine powder. Freeze fracturing with the BioPulverizer is also useful for extracting labile tissue metabolites and as a preliminary step to other homogenization techniques.
INSTRUMENTS
Mixing & Stirring
Vortex Mixer
The UNC-VM1000 Vortex Mixer is a heavy duty, variable speed mixer for a variety of different laboratory applications. It features a 2-position switch for continuous or touch-on modes and a soft rubber-mixing cup for single tube mixing. Dimensions: Weight: 6.2" x 5.0" x 6.5" 9.4lbs
VX-100 Vortex Mixer

Specifications Speed Range: 0-3000rpm Operation Mode: Touch or Continuous Ambient Operating Range: 4C-65C Dimensions: 12cm x 15.5cm x 13cm Weight: 3.0kg Electrical: 220V, 50/60Hz
Cimarec Ceramic Top Stirrers

Available in 4 x 4 7 x 7 12 x 12 The solid ceramic top plate is easily cleaned & resistant to acid and alkali, while a reflective white colour enhances sample visibility A strong magnetic coupling ensures stirring bar stays locked with drive magnet, even in viscous solutions. The rugged cast aluminum body provides stability and durability. A power light indicates when power is supplied to the speed control. Quiet stirring is achieved using a directdrive motor/magnet system. Stirrers come complete with one (2 x .38 diameter) Teflon-coated stirring bar, (Cimarec 1 supplied with one 1 x .31 diameter stirring bar), and grounded three-wire cord and plug.
The VX-100 vortex mixer is a valuable addition to any laboratory. The continuously adjustable speed control meets a variety of applications. Lower speed settings allow for gentle mixing of samples while higher speeds can be used for vortex mixing and resuspending of pellets. The three position control switch can be set for continuous operation or touch control. The general purpose cup attachment supplied with the VX-100 accepts a variety of different sized tubes. A variety of head attachments are available. The heavy duty construction of the unit ensures a long life and combined with the rubber feet prevents walking across the bench during operation.
Vortex Mixer
Specifications Speed Range: Variable to 3,000rpm Temperature Range: 2C to 40C Electrical: 220V, 60Hz Dimensions: 5H x 6D x 5W
Cimarec Ceramic Top Hotplates

Features: Solid ceramic top plate is easily cleaned and resistant to acid and alkali. Cimarecs reflective bright white color enhances sample visibility. Three sizes available: 4 x 4, 7 x 7, and 12 x 12. Integral ring-stand holder accommodates 0.5 diameter support rod. Infinite temperature selection is provided by a percentage input controller. Units reach maximum temperature of 538C in eight minutes. Accommodates sample weights up to 25lbs. on the 7 x 7 and 12 x 12 models and up to 10lbs. on 4 x 4 models. Stirring models have a strong magnetic coupling to ensure that the stir bar remains locked with drive magnet, even in viscous solutions.
Our Vortex Mixer blends fluids quickly and thoroughly with a true vortex action. Variable speed settings allow for precise mixing from a gentle shake to an aggressive vortexing action. The mixer has a durable aluminum housing with non-slip rubber feet and can be set for continuous operation or touch start and stop. Unit comes complete with cup-head for single test tubes and a 3 platform head design for beakers, flasks, and multiple test tubes. A variety of adapters are also available to increase the vortexing capacity.
Nutating Mixer
This high quality Nutating Mixer can be used for a variety of applications including mixing or resuspension of specimens, tissue culture, immunoblots, etc. The mixer features variable speed from 5 to 40rpm and operates at a 20 tilt angle. Fits either a 5" x 9" table (for use with various tube sizes) or an 8" x 8" table (for microwell plates, blots, petri dishes, and cell flasks). The unit comes standard with the 8" x 8" table.
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Orbital Shakers, Rockers, 3-D Rocker

BIOSHAKER PLUS
INSTRUMENTS
Shakers
Orbit Digital Orbital Shakers By Labnet

The Orbit line of digital orbital shakers is designed to satisfy the most demanding mixing and vortexing requirements across a broad range of applications. Heavy-duty construction combined with powerful motors and advanced electronic controls enable the shakers to withstand the stresses of continuous operation, even in a cold room or incubator. All models feature continuously variable speed controls, a wide assortment of accessory attachments, and can be set for timed or continuous operation.
Speed Range: 25-250rpm Platform Size: 11" x 11" Dimensions: 10.4" x 9.2" x 6.0" Weight: 9.8lbs Orbit 1.9cm
Orbit Models M60, P2 & P4 Microplate & Microtube Shakers

Speed Range: 100-1,400rpm Timer: 99.5 minutes or continuous Orbit: 3mm Max. Load:0.3kg Dimensions: 17 x 28 x 15cm
BIOSHAKER II Heavy Duty

20" x 20" Platform Skid Free Polyurethane Mat Rugged construction One year Warranty Parts and Labor Load capacity to 15 lbs 40-400RPM Suction cup feet (Cant Dance)
The BioShaker Plus is a compact, durable, variable speed orbital mixer that will accommodate a wide variety of printed well slides, culture plates, flasks and small test tube racks, as well as RPR and VDRL slides or 96 well microtiter (immunoassay) plates. It features a 11"x 11" mixing platform with a removable/replaceable rubber pad, variable speed of 25-250rpm, a tachometer with an analog rpm indicator (adjustable), 3 position switch for Constant On/Off/Timed Mixing, indicator alarm at end of timed mixing cycle, and hold down spring for small flasks.
The Orbit P2 and Orbit P4 hold two and four microplates respectively, by means of a contoured stainless steel platform with retaining springs. The Orbit M60 holds 60 x 1.5/2.0ml microcentrifuge tubes (or 0.2ml and 0.5ml tubes with optional insert adapters).
For Gels & Blots Single Or Double Rocker

New Rockers are up to 10 times quieter. Adjustable tilt from 0 to 40 Speed is adjustable from 4 to 160 rocking motions per minute. Solid-state speed control Non-slip rubber on platform decks. Operates in temperatures from 0C to 65C. Available in single & double platforms.
Orbit Model 300 Platform Shaker
Speed Range: 100-1,200rpm Timer: 99.5 minutes or continuous Orbit: 3mm Max. Load: 2kg Dimensions (W x D x H): 26 x 32 x 13cm
Gyrotwister GX1000
The Orbit 300 accepts flasks, dishes or plates by means of interchangeable platforms and is ideal for most mixing, agitation, and vortexing applications. The flask platform has spring-loaded retaining bars, making it flexible enough to accept a variety of different vessels. Different platform configurations available: for microplates, with nonslip mats for holding dishes, for flasks, racks for holding tubes of various diameters. Three dimensional motion Variable Speed range (6-60 rpm) Non-slip rubber mat Single, double, large and extra large platforms available (33 x 30 cm) Dimpled rubber mat (included) holds tubes in place Elastic tie downs (included) to secure various containers Safe for use in controlled environments (4 to 65C) Timer can be set for up to 3 hrs or continuous
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Water Baths - General, Shaking, Reciprocating

Lab-Line Aquabath Waterbaths
Standard Aquabaths are controlled by an adjustable hydraulic thermostat. A second independent hilimit thermostat assumes control of bath temperatures should the control thermostat fail. Temperature is maintained even if the bath accidentally runs dry, thereby eliminating heater burn out. Individual pilot lights indicate which thermostat presently controls temperature. Bath temperature is read from a glass thermometer (not supplied). Digital Aquabaths feature digital set temperature. Temperature is read from the LED display. Each Aquabath can be used as a boiling bath with the addition of the Plexiglas gable cover (included). Available in 2L, 3L, 6.7L, 14.6L, 22L, 33L, 44L capacities in both the standard and digital formats. Dual chamber baths are 14.6L (large chamber) and 6.7L (small chamber). Gable covers available separately.
INSTRUMENTS
Water Baths
Premium Water Baths

Ideal for all general laboratory applications, the Premier Water Baths utilize an integrated PID microprocessor controller for easy temperature selection, rapid heatup, and excellent stability. The seamless, stainless steel tank has rounded corners for easy cleaning and to minimize contamination. All controls are recessed to protect from spills and there is an adjustable over-temperature safety cutout. Supplied with a stainless steel gabled lid, diffuser tray, and shelf. Model: Tank Volume: Tank Dimensions (LxWxH): PB-600 6.0L 6" x 11.8" x 5.5" PB-1400 14.0L PB-2800 28.0L PB-3600 36.0L
12.8" x 11.8" 20" x 11.8" x 25" x 11.8" x x 5.8" 7.5" 7.5"
Temperature Range: Ambient + 5C to 100C in 0.1C increments Temperature Uniformity: 0.2C
SWB 5050 Reciprocating Shaking Water Bath

The SWB 5050 is an intelligent shaking water bath designed to meet the needs of todays biological laboratory. Its broad temperature range makes it useful for a variety of applications, from growing bacterial cultures, to hybridization, to denaturation of DNA samples. Specifications: Temp. Range: Ambient +5C to 85C (to 105C with stainless steel gable cover) Temp. Stability: 0.1C Temp. Control Microprocessor, digital display Overtemp. Safety Adjustable Bath Volume: 50L Drain Tap Shaking Speed Chamber Construction Lid Construction Timed Operations Heating Capacity Flask Capacity Platform Height Built in with front mount drain hose Variable, 20 to 200 strokes/min. Corrosion resistant stainless steel Clear acrylic, hinged to housing Max. 100 hours or continuous 2000W 4 x 2L, 6 x 1L, 9 x 500 ml, 16 x 250 ml, 50 x 100 ml Adjustable, 10-90 mm
ORS-200 Orbital Bath Shaker
&
Reciprocating
Water
The ORS-200 water bath shaker combines both reciprocating (linear) and orbital shaking motions into one unit. Temperature and shaking speed are digitally set and displayed, with the control panel recessed to protect from spillage and accidental alteration. Each bath is constructed of a stainless steel tank, with bottom drain, in a durable outer case. The heater and temperature sensors are mounted underneath the tank and there is an adjustable safety overtemperature cutout. To change from orbital to reciprocating motion, simply change the orientation of the tray. Stainless Steel lid available separately.
Specifications: Tank Volume: Temperature Range: Temperature Uniformity: Shaking Speed: Orbital Radius: Strokes per Minute: Linear Adjustable Stroke Length: Shaking Tray Size: Tank Dimensions: 28.0L 0 to 99C in 0.1C increments 0.1C 20 to 200rpm (Orbital) 9mm 40 to 360 (Reciprocating) 18mm, 28mm, 36mm 14.75" x 9.25" 19.75"L x 11.75"W x 7.75"H
Lab Companion Shaking Water Baths

Features: Digital PID Microprocessor Controller Patented CLS (Custom Logical Safety) System Volume Capacity 17L / 25L / 37L / 55L Auto Tuning function for exact temp. control 99h 59m Timer with Time Delay ON-OFF Temperature Range Amb.+ 5.0 ~ 100.0 C 20 to 180 rpm Shaking Speed Reciprocating motion Shaking amplitude 25, 30, 35mm (30mm Standard) Standard offer : Complete Spring Wire Rack & Stainless Steel Gable Cover Optional : Test Tube Rack , Universal Platform for mounting Flask Clamp, Spring Wire Rack, Handy Cooler
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Refrigerated & Heating Circulators

Economy Series Refrigerated Heating Circulators
INSTRUMENTS
Water Baths
TopTech Series Refrigerated Heating Circulators
Model: Bath Volume: Bath Dimensions (W x L x D): Operating Temperature Range: Temperature Stability: Heating Capacity: Cooling Capacity @ 20C: Pump Pressure Capacity: Pump Flow Capacity: Overall Dimensions (W x L x H):
F25-EC 4.5L 5" x 5.5" x 6" -25 to +100C 0.03C 1000 watts 350 watts 5 psi 15 lpm 9.1" x 16.6" x 23.6"
F34-EC 20L 9.5" x 12" x 6" -20 to +95C 0.03C 1000 watts 450 watts 5 psi 15 lpm 15" x 23" x 25" The TopTech Series Refrigerated Heating Circulators feature PID temperature control. High and low temperature warnings are standard, as well as a high temperature safety cut-off and low liquid level protection. For programmability, units can be connected via the RS232 port to a computer. Baths are constructed of stainless steel. Model: Model: Bath Volume: Operating Temp Temp Stability: Heating Capacity: Cooling Cap: Pump Pressure Cap: Pump Flow Cap: F25-EC F25-MD 4.5L -25 /+200C 0.01C 1000 watts 350 watts 5 psi 5 lpm F34-EC F34-MD 20L -30/150C 0.01C 1000 watts 450 watts 5 psi 15 lpm
TopTech Series Open Bath Heating Circulators MP Series
6 Liter Mini Water Bath

Labnets 6 Liter Water Bath is solidly constructed with a stainless steel, seamless chamber that resists corrosion. A stainless gable cover is included to minimize evaporation. The optional non-toxic (non-mercury) thermometer clips onto the gable cover for accurate temperature monitoring. The chamber accommodates plastic tube racks. Specifications: The MP Series Open Bath Heating Circulators are a step up from the EC Series. Digital temperature control via a splash-proof keypad, high and low temperature warnings, bright LED temperature display, low liquid level protection, an RS232 interface, and high temperature safety cutoff are just a few of the standard features. Baths are constructed of stainless steel and feature a working temperature range of 20C to 100C. Baths constructed of Plexiglas or Makrolon are also available. Model: Bath Volume: Bath Dimensions (W x L x D): MP-5 5L 6" x 6" x 6" MP-13 13L 7" x 12" x 6" 20-100C 0.02C 1000 watts 1.75 psi 10 lpm 15"x13"x14" MP-19 19L 4" x 12" x 6" 20-100C 0.02C 1000 watts 1.75 psi 10 lpm 22"x13"x14" Exterior dimensions (W x D x H) Interior dimensions Weight Chamber capacity Temperature range 14x11x13 in 12.6x6.7x6.9 in 17 lbs / 7.7 kg 6 liter +5 C to 100 C
Operating Temperature Range: 20-100C Temperature Stability: Heating Capacity: Pump Pressure Capacity: Pump Flow Capacity: 0.02C 1000 watts 1.75 psi 10 lpm
Overall Dimensions (W x L x H): 7"x13"x14"
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Hybridization Ovens & Accessories

ProBlot 6, 12 and 24
These standard ovens meet the capacity demands of medium to high throughput laboratories. The standard rotisseries in these ovens are unique in that they will hold 50ml tubes, eliminating the need to purchase a separate rotisserie for processing strips and small blots. The ProBlot 24 features two independently controlled chambers, allowing for separate applications to be carried out simultaneously within the same unit. All three of the standard ovens feature a corrosion resistant stainless steel interior. Optional accessories include a rocking platform, rotisserie for 70mm bottles, and a rotisserie for vertically positioned 50ml tubes.
INSTRUMENTS
Hybridization Ovens
Hybridization Mesh and Bottles
The availability of the accessories combined with the broad temperature range of these ovens, makes them useful for hybridization and washing of blots as well as other applications requiring strict temperature control
Specifications: ProBlot 6 Catalog number Bottle capacity Rotisserie/rocker speed Temp. range Temp. control/display Temperature resolution/ Uniformity Exterior dim. (WxDxH) Chamber dim. (WxDxH) H-0600 6 large, 12 small 4 to 20rpm Ambient + 5 to 80C Microprocessor/ digital 0.1/0.5C ProBlot 12 H-1200 12 large, 24 small 4 to 20rpm Ambient + 5 to 80C do 0.1/0.5C ProBlot 24 H-2400 24 large, 48 small 4 to 20rpm Ambient + 5 to 80C do 0.1/0.5C
Mesh sheets may be used to facilitate membrane handling and improve overall results. They keep membranes from overlapping, support them during handling, and allow larger numbers of membranes to be hybridized effectively in a single bottle. Available in Rolls and Sheets. Our high strength glass bottles are available in many size configurations to suit various hybridization applications efficiently. Manufactured of low beta emitting borosilicate, they reduce exposure to radioactivity. Exposure is further limited by the leak-proof closure system. Red Cap - Temperature Resistance: -45C to +200C (Teflon Seal) Blue Cap - Temperature Resistance: -40C to +140C (O-Ring Seal) Available in different sizes.
HL-2000 HybriLinker
18.4x17x17.4 in. 18.4x17x17.4 in. 20x16.5x27.7 in. 14.4x11x11.3 in. 14.4x11x11.3 in. 14.4x11x11.3 in.
All ProBlot Hybridization Systems are supplied with a starter kit containing 2 of Labnets ProBlot bottles and a pack of hybridization mesh. The bottles are constructed of heavy walled borosilicate glass with a flat bottom. The 35mm diameter bottles are available in a variety of lengths.
The innovative HL-2000 HybriLinker System combines the benefits of a hybridization oven and a UV crosslinker (254nm) in one self-contained unit. Hybridizer Features: Temperature control (ambient to 100C), variable speed control (10 to 18rpm), offset bottle-positioning, multiple bottle sizes (twenty 35 x 150mm, ten 35 x 300mm, or six 75 x 300mm tubes, or different arrangements of each), stainless steel internal construction, and optional stainless steel rocker plate. Also available is an optional over-sized bottle rotisserie, which accommodates six 70 x 300mm bottles. Crosslinker Features: Microprocessor controlled UV feedback system, preset or user-selected UV energy exposure or UV time exposure, shortwave 254nm UV, maximum UV energy setting of 999.900 microjou
HB-500 Minidizer
The HB-500 Minidizer is a personal, desktop hybridization oven that is ideal for use in laboratories with low use and limited space requirements. Microprocessor controls allow for consistent, repeatable results and the chamber interior is stainless steel with a 3/8" beta-blocking acrylic cover. The cover swings down for direct access to the bottle carousel, which can be removed from the chamber for the easy insertion or removal of bottles. Ambient to 80C 12rpm Four 35 x 150mm Eight 50ml or 15ml Conical Tubes 9"H x 13"W x 8"D
Minidizer Specifications: Temperature Range: Rotation Speed (Fixed): Bottle Sizes Accepted: Dimensions:
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Hybridization Ovens/ Electroporation Cuvettes

ProBlot 12S
More than just a hybridization oven, the ProBlot 12S has an orbital shaking mechanism built-in to the base, expanding its use to a variety of other applications. The rotisserie in the ProBlot 12S will hold 12 large or 24 small bottles as well as 50ml tubes. The base of the shaker accommodates plates and dishes. The flask platform, available separately, is supplied complete with 6 x 250ml flask clamps. An optional rocking platform is also available for use in the chamber when the rotisserie is removed. Optional accessories include the flask platform with clamps, rocking platform and rotisseries for other sizes of bottles Specifications Temperature range Bottle capacity Temperature control/display Rotisserie/rocker speed Temperature resolution/uniformity Shaker speed Exterior dimensions (WxDxH) Timer Chamber dimensions (WxDxH) Ambient +5 to 80C 12 large, 24 small Microprocessor/digital 4 to 20rpm 0.1/0.5C 0 to 300rpm 18.4x17x17.4 in. 3 hours max. with hold 14.4x11x11.3 in.
INSTRUMENTS
Hybridization Ovens/ Electroporation Cuvettes
Our range of HiMaX Electroporation cuvettes are designed to maximise molecular electroporation and electrofusion efficiencies for Bacteria, Yeast, Insect, Plant and Mammalian cells. Each batch of cuvettes has to undergo rigorous testing at several stages during the manufacturing process for engineering tolerances, biocompatibility and sterility, prior their being Quality tested for optimal and reproducible impedance measurements. COMPATIBILITY The cuvettes are compatible with most electroporation systems BIO-CONTROLLED All batches are checked to optimize the Bio and Transfection compatibility, with stringent use of high quality grade polycarbonate and High grade chemicals to ensure consistent uniform pulse generation and improved gene transfer. HIGH TOLERANCE MOULDING The moulding process ensures extremely high tolerances so that the electrodes have a consistent gap and parallel configuration. The electrodes are also cleaned chemically and physically to fully optimize the cuvette for high transformation efficiencies. LOW DEAD VOLUMES All 1mm and 2mm cuvettes have a tapered V bottom so that reduced sample volumes can be used while aiding sample pick up and minimizing dead volumes.
All ProBlot Hybridization Systems are supplied with a starter kit containing 2 of Labnets ProBlot bottles and a pack of hybridization mesh.
ProBlot Jr.
The ProBlot Jr. is a unique, personal sized oven. Requiring less than one square foot of benchspace, it has a capacity for four small bottles. The oven is portable and easily moved as needed. The acrylic door opens upward for easy sample access. Optional rotisseries hold 15 and 50ml tubes. Standard accessories include two small bottles, one pack of mesh and a 35mm rotisserie.
Cat. No. EP-101
Product 1mm cuvette individually wrapped and sterile 1mm cuvette with long electrode individually wrapped and sterile 2mm cuvette individually wrapped and sterile 2mm cuvette with long electrode individually wrapped and sterile 4mm cuvette individually wrapped and sterile Disposable sterile individually wrapped plastic pipettes
Qty
50 Nos.
EP-201
50 Nos.
Specifications Bottle capacity Rotisserie/rocker speed Temperature range Temperature control/display Temperature resolution/uniformity Exterior dimensions (WxDxH) Chamber dimensions (WxDxH)
ProBlot Jr. 4 small 4 to 20rpm Ambient +5 to 80C Microprocessor/digital 0.1/0.5C 10.75x11.25x7.25 in. 6.5x6.x6.5 in.
EP-102
50 Nos.
EP-202
50 Nos.
EP-104
50 Nos.
EP-101
50 Nos.
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UV Transilluminators and Crosslinkers

Benchtop UV Transilluminators
Economical benchtop models provide midrange (302nm) UV in either single intensity or High/Low Intensity settings. Select the High setting for analytical documentation work or the Low setting for reducing photonicking or photobleaching of gel samples while doing preparative work. The 2UV and 3UV Benchtop Transilluminators deliver multiple UV wavelengths in one compact unit. The 3UVs patented design provides 302nm, 365nm and 254nm in one compact model - excellent for premium lab space. Work surface area: 20x20cm. Longwave UV Ideal For Viewing Stained Gels Midrange UV Provides A Wavelength Sensitive For Nucleic Acid Visualization; Increased Fluorescence For Photodocumentation Work Shortwave UV Can Be Used To Irradiate Samples Compact Version Provides Three Ultraviolet Wavelengths In One Unit Dimensions for all Benchtops (Except 3UV): 13.25W x 9.5D x 4.75 H 3UV Dimensions: Model M-15 M-15E M-20 M-20E M-26 M-26E LM-20E LM-26E LMS-20E LMS-26E Description Hi/Lo Intensity, 220V Single Intensity, 220V Hi/Lo Intensity, 220V Single Intensity, 220V Hi/Lo Intensity, 220V Single Intensity, 220V 2UV, 220V 2UV, 220V 3UV, 220V 3UV, 220V 14W x 11D x 6H Filter Size 15 x 15cm 15 x 15cm 20 x 20cm 20 x 20cm 21 x 26cm 21 x 26cm 20 x 20cm 21 x 26cm 20 x 20cm 21 x 26cm Wavelength 302nm 302nm 302nm 302nm 302nm 302nm 302nm/365nm 302nm/365nm 254nm/302nm/365nm 254nm/302nm/365nm
INSTRUMENTS
Transilluminators/Crosslinkers
UV Crosslinkers
Preset And Manual Controls For UV Or Time Exposure Settings Internal Microprocessor Measures And Controls UV Output, Insuring Maximum Energy Efficiency Internal UV Sensor Is Calibrated With Traceability To NIST Standards Use shortwave for sterilization, bonding DNA; use midrange for gel electrophoresis; use longwave for UV curing. LED display above the touch panel continuously displays remaining time or energy settings. Clear door window allows viewing of the process while blocking the UV radiation. A safety shut off system further protects the user from exposure to UV. Available in 254 nm, 302 nm and 365 nm. UV wavelength is interchangeable in any model by changing the UV tubes and calibrating the unit to the new wavelength with the UV sensor (ordered separately).
UV/White Light Transilluminators

UV/White Light Transilluminators give white light and 302nm midrange UV side by side for viewing Coomassie Blue stained gels, silver stained protein gels, autoradiographs and microtiter plates on white light side. UV side can be used for viewing gels such as ethidium bromide stained gels. UV Blocking Cover included. Transilluminators Can Be Used With GDS & Doc-It Systems. The filter size is 20x20 cm and avaioable in 302nm/white, 365 nm/white and 302/ 365nm/White. Model TLW-20 utilizes longwave and white light side by side. Model LMW-20 houses both longwave and midrange on one side and white light on the other, providing varied wavelengths for different applications. Easily switch between light sources with the rocker switch. Dimensions: 19W x 13.25D x 5 H. Replacement tubes available.
UV/White Light Converter Plates

A specially designed plate converts a transilluminators ultraviolet radiation to white light for viewing protein gels; Coomassie blue stained or silver stained media. The Converter Plates uniquely phosphored Plexiglas assembly (patent pending) converts the UV radiation via a white diffuser. The Plexiglas is mounted in a durable scratch-resistant metal housing for durability. Handles are situated on two sides of the plate for easy handling. Sizes available are 21x26 cm and 20x40 cm
High Performance UV Transilluminators

The higher watt tubes allow the tubes to be moved further from the filter surface resulting in increased light diffusion while at the same time maintaining the high UV intensity. Models are available with midrange or longwave ultraviolet. Dimensions: 19.13W x 13.25D x 5.63H (Height includes cover) Replacement tubes available. Model TFM-20 TFM-26 TFM-30 TFM-40 TFL-40 Cat. No. 95-0286-01 95-0285-01 95-0302-01 95-0283-04 95-0283-01 Volts 220 220 220 220 220 Intensity Hi/Lo Hi/Lo Hi/Lo Hi/Lo Hi/Lo Wavelength 302nm 302nm 302nm 302nm 365nm Filter Size 20x20cm 21x26cm 25x30cm 20x40cm 20x40cm
Visi-Blue Converter Plates

A specially designed plate converts 302nm to 480nm blue light for use with SYBR Green, SYPRO Orange, and GFP stains. Sizes available are 21x26 cm and 20x40 cm.
Handheld UV Lamps
These Handheld UV Lamps feature an ergonomically designed handle for easier handling and comfort. They are available in longwave, shortwave, midrange, or multiband longwave/shortwave UV models. An optional stand is available for benchtop use and the lamps can also be used with the C-10 ChromatoVue Viewing Cabinet for viewing materials in a darkened environment.
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Cuvettes, Gloves, Labels

Cuvettes, Semi-Micro (1.5 ml) Polystyrene (VIS)
These exceptionally fine quality polystyrene cuvettes are deisgned for assays throughout the 340nm to 750nm visible spectral range. A standard 1cm light path and recessed windows provide maximum light transmission.1 pack contains 50 nos. Dimensions: Windows: Light Path: 12.5mm x 12.5mm x 45mm 5mm x 23mm 10mm
INSTRUMENTS
General Lab Equipments
Diamond Grip Plus

For those who prefer the grip of a fully textured glove, Diamond Grip Plus is the glove of choice. And for those healthcare professionals who require greater tactile sensitivity for instrument manipulation or administering medical care, Diamond Grip Plus is a perfect fit. Diamond Grip Plus is powder-free to help reduce the risk of skin irritation. Quantites: 100 Gloves Per Dispenser Box; 10 Boxes Per Case
Small, Medium, Large
Cuvettes, Macro & Semi-Micro Polystyrene (VIS)

Premium grade polystyrene cuvettes fit most spectrophotometers, including Beckman, Perkin-Elmer, Gilford, Hitachi, Varian, and Shimadzu. Both the 4ml (macro) and the 1.5ml (semi-micro) have two clear sides. Applications include general spectroscopy in the visible region, physician office analyzers, and enzyme reaction rate studies. 1 pack contains 1000 nos.
Cryo-Babies & Cryo-Tags Labels For Cryogenic Storage

Cryo-Babies and Cryo-Tags will withstand conventional freezing and cryogenic storage in vapor-phase and liquid nitrogen. These heat resistant, ultra-flexible polyolefin labels with acrylic adhesive are chemically inert and adhere to all plastics, glass, and metal. The labels will withstand boiling water baths at 100C and dry heat up to 150C without cracking, peeling, or degrading. They are water and moisture resistant, and will resist water soaking for at least 24 hours at room temperature.
Cuvettes, Semi-Micro (1.5ml) Methacrylate (UV-VIS)

Injection molded from a superior grade of methacrylate, these cuvettes designed for accuracy throughout the UV-VIS spectral range from 285nm to 750nm. Excellent optical qualities throughout the spectral range make them perfect for scanning small volumes, enzyme rate reactions, and standard curve determinations. 1 pack contains 50 nos.
Cloth Glove Liners

These 100% cotton glove liners fit comfortably under plastic gloves to ensure maximum comfort while protecting sensitive skin from the irritating effects of latex or donning powders. Available in packs of 1 Gross, in small, medium and large sizes.
Tough-Spots Pre-Cut Peel-Off Round Labels for Microcentrifuge Tube Tops

Tough-Spots are chemically inert white polyvinyl labels that adhere to all plastics. These labels withstand boiling water baths, conventional and cryogenic freezer temperatures, most organic solvents, caustic agents, and other challenges without peeling, becoming brittle, or degrading. The 3/8 Spots will fit 0.52.0ml microtubes and the 1/2 Spots are ideal for 1.5-2.0ml containers.
Gloves Shark Skin The Powder Free Latex Glove

Non-Sterile Superior Texture Puncture Resistant Sleek Fit Jaw-Like Grip Even Wet Durable and Comfortable Quantites: 100 Gloves Per Dispenser Box; 10 Boxes Per Case Small, Medium, Large
Available in different colours.
Tough-Tags The Pre-Cut, Peel-Off Labels Perfectly Sized to Fit Microcentrifuge Tubes and Other Laboratory Containers
Temperature Resistant (can be used in autoclaves, boiling water baths and freezers Resists Solvents & Caustic Reagents Ideally Sized Centrifuge Compatible Scuff & Smear Resistant Multiple Uses The 1.28" x 0.50" size is ideal for 1.5-2.0ml microtubes while the 0.94" x 0.50" size fits 0.5-2.0ml microtubes. Different varieties of Tough-Tags are available for different applications. Laser Tough Tags used for printing directly from Laser printer. Teeny tough tags used for 0.2 ml PCR tubes, Sidewall tags used for walls of microplates, petri dishes and their lids. General-purpose tags are larger and used for a wide variety of laboratory applications.
Diamond Grip
Diamond Grip is our most popular powder-free, textured exam glove. It stands alone as a perfect glove for any application. Its built tough for greater reliability and comfort. Plus, its powder free to help reduce the risk of skin irritation. Quantities: 100 Gloves Per Dispenser Box; 10 Boxes Per Case Small, Medium, Large
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Small and Important tools that you need in the lab.

The Bioslide Advantage Replica Plating Device
INSTRUMENTS
General Lab Equipments
The Replica Plating Device utilizes a locking ring that secures sterile velveteen squares onto a PVC cylinder for easy control of the plating device. It may be disinfected between uses with a brief rinse in ethanol or chlorine bleach. The aluminum ring is 102mm in diameter and the PVC cylinder is 55mm high. For use with 90-100mm petri dishes. Includes one bag of 12 Velveteen Squares (6" x 6"). These squares are also available separately.
Precision CT - Clean Ready for surface chemical modification and coating process Precision CT - Amine Stable, covalent coupling of primary amine on slide surface Ready for DNA/RNA printing Quality tested for oligonucleotide immobilization Ideal for custom in-house printing Precision A - Aldehyde Stable, covalent coupling aldehyde on slide surface Modified with primary amines form Schiff-based covalent bonds Ideal for protein immobilization Precision E - Epoxy Stable, covalent coupling epoxy on slide surface Superior attachment of amino-modified oligonucleotides Ideal for synthetically fabricated oligonucleotides and PCR products Precision L - Lysine Ready for DNA/RNA printing Hand screened and tested Ideal for custom in-house printing Starting with high quality low fluorescent glass, the Bioslide UltraClean, Amine, Aldehyde, Epoxy, and Poly Lysine-coated slides are manufactured in a Class 100 Cleanroom and have been tested to ensure surface uniformity, consistent contact angle and slide-to-slide reproducibility during spotting and hybridization processes. Moreover, the nearly particle-free environment ensures high-quality surface treatments which bind an optimal amount of probe, optimize volume transfer, minimize nonspecific binding of labeled targets, promotes uniform spot morphology, and assures the consistent performance of every pre-coated slide.
Innoculating Loops and Needles

Our Innoculating Loops and Needles are smooth and flexible to facilitate uniform, smooth streaking without damage to media/gel surfaces. The needles are straight and suitable for removal of single colony specimens. The loops are extra-smooth and constructed of polypropylene and rubber. Both the loops and needles are packed sterile in safe, tamper-proof, zip-seal resealable bags. Cross contamination due to improper sterilization is eliminated. They can be used under hoods without danger, are color coded for ease of size identification, and are extra long to reduce the risk of contamination. Total Length: 22.7cm (9) Loops available in 1l and 10l.
Lazy L Spreaders
Made of durable polystyrene. Unlike glass alternatives, disposable Lazy L Spreaders are non-breakable and do not pose a safety hazard. L-shaped design for convenient and lazy spreading; just turn the petri dish 360 degrees to provide smooth and even sample distribution. Pre-sterilization eliminates flaming or autoclaving and reduces the risk of contaminations. No more wasted time waiting for glass or metal spreaders to cool before use.
Gel Tools
UVPs Gel Tools are useful for researchers working with gels on transilluminators. The Gel-Cutters edge allows for cutting and removal of gel material. The Gel-Scooper, made of strong acrylic with a beveled edge, is designed for easy transfer of gels from electrophoresis equipment to viewing equipment. Gel-Trays, made of UV transmitting Plexiglas, can be used for moving gels to the transilluminator. The tray protects the transilluminators filter surface from scratches. Side panels on the Gel-Tray, angled at a 45 bend, extend upward from the tray surface for easy handling. The Gel-Ruler has centimeter markings that fluoresce under 365nm and 302nm UV wavelengths providing reference marks for DNA analysis.
UV Protective Eyewear
Eye and face protection are essential for anyone working with ultraviolet light sources. Choose from three styles of comfortable eye/face protection: spectacles, goggles, or faceshield. Spectacles are made of impact resistant polycarbonate. Goggles are constructed of speciallyformulated plastic giving optimum viewing contrast and lessened eye fatigue. Air vents are located on each side of the goggles to allow air flow while blocking UV. The faceshield covers the face and neck area.
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Crystal Screening Kits

JBScreen Crystal Screening Kits
The JBScreen Crystal Screening Kits 1-10 cover 240 of the most prominent buffers for protein crystallization. Their compositions result from data mining of several thousands of crystallized proteins. JBScreen represents the statistically most successful buffers that yielded protein crystals suitable for X-ray diffraction. Just check out the formulations of JBScreen, you will probably recognize your conditions or very similar ones. The JBScreen buffers are principally ordered by type and concentration of the precipitant. This allows easy extraction of all relevant information and is already a first step to a refinement: Once you get a hit, you immediately see the effects of the neighboring conditions. Subsequent fine-tuning of preliminary hits will be much more efficient. Each kit contains 24 buffers, delivered in 0.7 ml sterile aliquots, with 10 units per kit. Alternatively, the JBScreen Mixed kit contains one single shot of all the 240 buffers. The 6x4 format of the units allows a convenient 1:1 setup for 24 (or multiples thereof) well plates. Its easy to use every ampoule and the units package has a coordinate system (A1A6 ... D1D6), thus almost certainly eliminating errors. No pipetting, just break off the ampoules top and use the buffer aliquot JBScreen is always fresh and sterile unlike common bulk solutions Reproducible- no evaporation of solvent Product JBScreen Kit 1 JBScreen Kit 2 JBScreen Kit 3 JBScreen Kit 4 JBScreen Kit 5 JBScreen Kit 6 JBScreen Kit 7 JBScreen Kit 8 JBScreen Kit 9 JBScreen Kit 10 JBScreen Kit Mixed Qty 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit Cat. No. CS-201S CS-201L CS-202S CS-202L Product
X-RAY CRYSTALLOGRAPHY
Crystal Screens
JBScreen bulk kits

The JBScreen bulk kits contain all 240 solution of the JBScreen system in sterile 10 ml quantities. These kits are especially designed for users who prefer reservoir solutions that are different from the standard 0.7 ml format, such as labs that use crystallization robots. Cat. No. CS-101L CS-102L CS-103L CS-104L CS-105L CS-106L CS-107L CS-108L CS-109L CS-110L Product JBScreen 1 bulk JBScreen 2 bulk JBScreen 3 bulk JBScreen 4 bulk JBScreen 5 bulk JBScreen 6 bulk JBScreen 7 bulk JBScreen 8 bulk JBScreen 9 bulk JBScreen 10 bulk Qty 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit
JBScreen HTS
JBScreen HTS contains the formulations of the JBScreen system, adopted to fit the 96-well format. JBScreen HTS is designed for high throughput crystallization applications using multi-channel pipettes or crystallization robots. Each JBScreen HTS 96-well master block is pre-filled with 96 sterile crystallization buffers, with either 1.0 ml (S size) or 1.7 ml (L size) per well and is sealed with indexed cap mats. JBScreen HTS will enable you to combine high-throughput crystallization techniques with the most efficient and logical screen available!
Cat. No. CS-101 CS-102 CS-103 CS-104 CS-105 CS-106 CS-107 CS-108 CS-109 CS-110 CS-111
Qty 1 Kit 1 Kit 1 Kit 1 Kit
JBScreen HTS I S (1.0 ml per well) JBScreen HTS I L (1.7 ml per well) JBScreen HTS II S (1.0 ml per well) JBScreen HTS II L (1.7 ml per well)
JBScreen Refill Kits

The JBScreen Refill Kits 1 10 contain 24 x 10 sterile aliquots of the JBScreen buffers, supplied as 10-ampoule blocks, with 0.7 ml per ampoule. The JBScreen Refill Kits are ideal if you are already using JBScreen and would like to re-use its packages and 24-ampoule units. Simply break off the ampoules from the blocks and re-fill your JBScreen kits! Cat. No. CS-101S CS-102S CS-103S CS-104S CS-105S CS-106S CS-107S CS-108S CS-109S CS-110S Product JBScreen 1 Refill Kit JBScreen 2 Refill Kit JBScreen 3 Refill Kit JBScreen 4 Refill Kit JBScreen 5 Refill Kit JBScreen 6 Refill Kit JBScreen 7 Refill Kit JBScreen 8 Refill Kit JBScreen 9 Refill Kit JBScreen 10 Refill Kit Qty 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit CS-303L JBScreen Membrane 3 1 Kit Cat. No. CS-301L CS-302L Product JBScreen Membrane 1 JBScreen Membrane 2 Qty 1 Kit 1 Kit
JBS Membranes
The JBScreen Membrane Screens 1 - 3 cover 72 of the most promising reagents for crystallization of membrane proteins. Their composition was devised by analyzing crystallization conditions of successfully determined membrane protein structures. The JBScreen Membrane crystallization buffers are principally ordered by type and concentration of the precipitant. Like in the case of JBScreen Classic , this allows easy extraction of all relevant information and is already a fi rst step to a refi nement of the crystallization process: Once you get a hit, you immediately see the effects caused by the composition of the neighbouring conditions. Subsequent fi ne tuning of preliminary hits will be much more effi cient.
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Wizard Screens, PEG Screens, Buffers

Emerald BioStructures Screens
deCODEs Emerald BioStructures growth matrices cover a range of crystallization conditions of varying buffers, salts, precipitants, and pH. Sixteen different precipitants are used, including volatile reagents such as ethanol, non-volatile reagents such as PEG 8000, and salts such as ammonium sulfate. Each crystallization Matrix Contains: 48 unique formulations, 10 ml each, prepared with ultra pure chemicals and water (18.3 MOhm), followed by sterile 0.22-micron filtration into sterile polypropylene tubes. Two oils, 5 ml each of miscible light and heavy oils for use in modulating vapor diffusion rates.
Crystal Optimization
JBScreen Basic
One approach to find suitable crystallization conditions is the SparseMatrix method. This method involves screening with an intentional bias towards conditions, which have been proven successful in the crystallization of biological macromolecules. In 1991, Jancarik and Kim published 50 conditions, which were derived from previously crystallized proteins. They are designed to fit the 24-well plate format and like in all other JBScreen crystallization kits, cacodylate buffers were replaced by MES. JBScreen Basic contains 96 unique reagent mixtures for screening a wide range of pH and various salts and precipitants. Each condition of the four kits is supplied in 10 ml quantities. Furthermore, JBScreen Basic HTS contains all 96 conditions in a pre-filled deep well block.
Cat. No. CS-121 CS-122 Cs-123 CS-124 CS-203S
Product JBScreen Basic 1 (Diol and PEG based) JBScreen Basic 2 (PEG based) JBScreen Basic 3 (Polymer, Alcohol, salt based) JBScreen Basic 1 (salt based) JBScreen Basic HTS (1.0 ml per well) JBScreen Basic HTS (1.7 ml per well)
Qty 1 kit 1 kit 1 kit 1 kit 1 kit 1 kit
Wizard I + II
Emerald BioStructures Wizard Screens are highly effective random sparse matrices for the crystallization of biological macromolecules (proteins, nucleic acids, peptides and combinations thereof). Sixteen different crystallants and eleven different buffers, ranging from pH 4.5 to pH 10.5, ensure a broad sampling of crystallization space. Cat. No. EBS-WIZ-I EBS-WIZ-II EBS-WIZ-F EBS-BWZ Product Wizard I Wizard II Wizard I + II Wizard I + II (in 96 well format) Qty 1 Kit 1 Kit 1 + 1 Kit 1 kit
CS-203L
JBScreen Buffer Kits

JBScreen Buffer Kits are designed for convenient reproduction and optimization of crystallization conditions. The solutions can be used to reformulate conditions of the JBScreen family , e.g. JBScreen Classic, JBScreen Basic, JBScreen Cryo, and other commercially available crystallization screens. Furthermore, JBScreen Buffer Kits can be employed for the straightforward preparation of custom screen solutions for the refinement and optimization of initial crystallization conditions. The JBScreen Buffer Kit formulations will help to save time preparing accurate and high-quality reagents for the reproducible production of single protein crystals. The JBScreen Buffer Kits contain ready-made buffer solutions with preset pH values. JBScreen Buffer Kit Sodium Acetate, pH 3.5 5.6 JBScreen Buffer Kit Sodium Citrate, pH 3.7 6.0 JBScreen Buffer Kit MES, pH 5.6 6.7 JBScreen Buffer Kit HEPES, pH 6.8 8.2 JBScreen Buffer Kit Tris-HCl, pH 7.1 9.0
Ozma PEG-Series 48-Salt: 1K, 4K, 8K and 10 K

The Ozma PEG-Series 48-Salt Screens are formulated using one of four different molecular weight PEGs; 30% (w/v) PEG 1000, 20% (w/v) PEG 4000, 20% (w/v) PEG 8000, or 10% (w/v) PEG 10,000 plus 48 different salts. These protein crystal growth matrices provide excellent coverage of a broad range of PEG molecular weights (1K to 10K) at known optimal concentrations for protein crystal growth, in combination with 48 different salts. The cationic components of the salts cover Ammonium, Calcium, Lithium, Potassium, Sodium, Magnesium, and Zinc. The anionic components the salts cover for Acetate, Chloride, Fluoride, Formate, Iodide, Nitrate, Phosphate (mono- and di-basic), Sulfate, Tartrate, Thiocyanate, Citrate, and Isothiocyanate. Full Ozma PEG-Series 48-Salt in 2 x 96 Well Matrix Block Plates: Contains 192 unique formulations comprising all 4 sets of our Ozma PEG 1K, 4K, 8K, and 10K screens, each with 48 different salts. Used for the crystallization of biological macromolecules in PEG-1000, PEG-4000, PEG8000 and PEG-10000 by varying the salt type. Cat. No EBS-PEG-1 EBS-PEG-4 EBS-PEG-8 EBS-PEG-10 EBS-PEG-48F EBS-PEG-48BLK Product Ozma PEG 1K 48-Salt Ozma PEG 4K 48-Salt Ozma PEG 8K 48-Salt Ozma PEG 10K 48-Salt Full Ozma PEG-Series 48-Salt Full Ozma PEG-Series 48-Salt in two 96 Well Matrix Block Plates Qty 1 Kit 1 Kit 1 Kit 1 Kit 4 Kits 2 Blocks
Each buffer is provided as a 1.0 M stock solution and supplied in 10 ml volumes.
Cat. No. CO-101 CO-102 CO-103 CO-104 CO-105
Product JBScreen Buffer Kit Sodium Acetate JBScreen Buffer Kit Sodium Citrate JBScreen Buffer Kit MES JBScreen Buffer Kit HEPES JBScreen Buffer Kit Tris-HCl
Qty 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit
JBS Single Stocks

Single stock solutions of the JBScreen components, i.e. buffers, salts and precipitants, are ideal for the optimization of your crystallization conditions. JBScreen Single Stocks are ready for use: the concentration is adjusted and they are sterile fi ltered. Buffers, Salts, Precipitants available.
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Solubility, Pre-crystallization Screen , Detergents, Additives

JBS Solubility Kit
The JBS Solubility Kit is a pre-crystallization screen to improve the composition of the initial protein buffer solution prior to performing crystallization set-ups. Studies have shown that aggregation of the protein may inhibit nucleation and crystal growth. Therefore, the JBS Solubility Kit has been developed to investigate protein samples towards their homogeneity and monodispersity prior to crystallization trials, employing hanging drop vapour diffusion experiments combined with dynamic light scattering. The JBS Solubility Kit contains a set of 24 buffer solutions at different pH-values for setting up hanging drop vapour diffusion experiments in order to monitor the aggregation and precipitation of the protein sample, and a set of 14 additives used for further optimization employing dynamic light scattering. Cat. No. CO-310 Product JBS Solubility Kit Qty 1 Kit
Crystal Optimization
JBScreen Plus
To select the formulations of the JBScreen Plus solutions, numerous reports on the use of additives to improve the quality and size of macromolecular crystals have been evaluated. JBScreen Plus comprises 5 kits, including kosmotropic (structurestabilizing) and chaotropic (structure-disturbing) additives, salts, volatile and non-volatile organics and other compounds. Each kit contains 24 different additives, supplied in ready-made aliquots (100 l each, except the JBScreen Plus Volatiles kit, containing 1 ml each), with adjusted concentration and sterile fi ltered. All solutions come in tightly closing glass vials that will keep them fresh and sterile. Cat. No. CS-501 CS-502 CS-503 Product JBScreen Plus Kosmotropic JBScreen Plus Chaotropic JBScreen Plus Salts JBScreen Plus Additives JBScreen Plus Volatiles JBScreen Plus Complete all 5 JBScreen Plus kits for a special price Qty 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 5 Kits
ADDit Additive Screen in 96 Well Block Plate

The ADDit Additive Screen is a set of 96 different small molecule and salt solutions that have been found to be effective in aiding the crystallization of biological macromolecules. 1.0 ml each prepared with ultra-pure chemicals and water (18.2 Megohm-cm) followed by sterile 0.22 micron filtration into a sterile 96 deep well matrix block plate that is sealed with a sterile reusable matte cover. Cat. No. ADDIT-1 Product ADDit Additive Screen Qty 1 Block
CS-504 CS-505 CS-506
JBScreen Detergents
The JBScreen Detergents Kits 1 and 2 cover a great variety of detergents that have been successfully used for the crystallization of membrane proteins. Each kit contains 12 detergents, supplied as stock solutions at 5 or 10 times the reported CMC (Critical Micellar Concentration), with 100 or 200 l per compound. The JBScreen Detergents kits are ideal supplements to the JBScreen Membrane screens. This combination will enable you to screen a broad range of crystallization conditions, while concentrating on the most successful detergents and therefore making crystallization screening of membrane proteins much more effi cient and less time and sample consuming. Cat. No. CD-101 CD-102 Product JBScreen Detergents 1 JBScreen Detergents 2 Qty 1 Kit 1 Kit
pHat Buffer Screen

The pHat Buffer Screen is a set of 96 different buffer solutions encompassing 12 different buffer systems at 8 different pHs each ranging in steps of 0.4 pH units from 1.4 pH units about the buffer pKa. The buffers are organized according to their final pH so as to facilitate the setup of subsequent refinement screens. The pHat buffers can also simply be added to any other 96 formulation screen to add another chemical dimension to the search for optimized crystal growth conditions. pHat Buffers in 96 Well Block Plate: The buffers are delivered in a 96 deep well block plate filled with 1.7 ml of each buffer solution at 10x the recommended final concentration. Cat. No. PHAT-T1 PHAT-B1 Product pHat Buffer Screen pHat Buffers 96 well Block Qty 1 Kit 1 Block
JBSolution Detergent Test Kit

The JBSolution Detergent Test Kit is designed to optimize solubilization of membrane proteins. Compounds assembled in the kit range from ionic and nonionic to zwitter-ionic detergents. These detergents have non-denaturing as well as denaturing properties. The arrangement is based on years of experience. The Kit contains 4 ml stock solutions of 27 detergents with a concentration of 4% and 3 buffers at 1 M concentration (Tris-HCl, HEPES Sodium Salt, NaPB), each at two different pH-values (7.5 and 8.0). Cat. No. DK-101 Product JBSolution Detergent Test Kit Qty 1 Kit
JBS Methylation Kit

Surface engineering of biological macromolecules provides a powerful technique to deal with proteins which are reluctant to crystallize or which yield poorly diffracting crystals only. The JBS Methylation Kit offers a straightforward tool for the selective methylation of lysine residues . The method does not require laborious cloning/expression/ purifi cation but chemically substitutes the protons of the amino group of lysine residues with methyl groups.
Cat. No. CS-510
Product JBS Methylation Kit
Qty 1 Kit
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Heavy Atom Derivatization for Crystallography

JBScreen Heavy
The search for suitable heavy-atom derivatives is often a tedious process and usually requires screening of a broad range of heavy atoms. The JBScreen Heavy kit will shorten this process: It contains a collection of 24 of the most successful heavy-atom compounds, selected from data mining of heavy-atom derivatized protein crystals, which have been successfully employed in structure determination of biological macromolecules. Each of the 24 heavy-atom compounds is supplied in three identical solid aliquots. Cat. No. CH-101 Product JBScreen Heavy Qty 1 Kit
Heavy Atom Derivatization
Halogen-containing ATP-analogs
2I-ADP 2-Iodo-adenosine-5-diphosphate, Sodium salt 2Br-ADP 2-Bromo-adenosine-5-diphosphate, Sodium salt 8I-ADP 8-Iodo-adenosine-5-diphosphate, Sodium salt 8Br-ADP 8-Bromo-adenosine-5-diphosphate, Sodium salt 2I-ATP 2-Iodo-adenosine-5-triphosphate, Sodium salt 2Br-ATP 2-Bromo-adenosine-5-triphosphate, Sodium salt 8I-ATP 8-Iodo-adenosine-5-triphosphate, Sodium salt 8Br-ATP 8-Bromo-adenosine-5-triphosphate, Sodium salt 2I-AppNHp (2I-AMPPNP) 2-Iodo-adenosine-5-[(,)-imido]triphosphate, Sodium salt 2Br-AppNHp (2Br-AMPPNP) 2-Bromo-adenosine-5-[( , )-imido]triphosphate, Sodium salt 8I-AppNHp (8I-AMPPNP) 8-Iodo-adenosine-5-[( ,)-imido]triphosphate, Sodium salt 8Br-AppNHp (8Br-AMPPNP) 8-Bromo-adenosine-5-[( , )-imido]triphosphate, Sodium salt
Halogenated nucleotides as rational phasing tools for protein crystallography

For solution of the macromolecular phase problem, the most commonly used methods (multiple isomorphous replacement (MIR) and that of multiple wavelength anomalous dispersion (MAD)) still involve the incorporation of heavy atoms into protein crystals. After crystallization, finding such derivatives is the second major bottle neck in the determination of the 3D structure of bio-macromolecules. Most labeling procedures focus on the protein itself in a trial and error fashion. Halogenated ATP and GTP analogs however, provide an alternative method that allows rational incorporation of heavy atoms into a large number of physiologically relevant enzymes: In the human genome alone, estimated 5,000 to 10,000 proteins interact with ATP or GTP. The incorporation of iodine or bromine allows MIR or MAD phasing for proteins with molecular weights of at least up to 50 kDa. Importantly, for MIR experiments, such derivatives are likely to be isomorphous with the native crystal. The kinetics of binding of 2-halogenated ATP analogs to most enzymes so far investigated.
Halogen-containing GTP-analogs
8I-GDP 8-Iodo-guanosine-5-diphosphate, Sodium salt 8Br-GDP 8-Bromo-guanosine-5-diphosphate, Sodium salt 8I-GTP 8-Iodo-guanosine-5-triphosphate, Sodium salt 8Br-GTP 8-Bromo-guanosine-5-triphosphate, Sodium salt 8I-GppNHp (8I-GMPPNP) 8-Iodo-guanosine-5-[( ,)-imido]triphosphate, Sodium salt 8Br-GppNHp (8Br-GMPPNP) 8-Bromo-guanosine-5-[( , )-imido]triphosphate, Sodium salt
JBS Halo Kits

Since it is difficult to predict a priori which one of the halogenated nucleotides will give the highest quality co-crystals, with this kit you can screen them all in parallel.
Other halogen-containing nucleotide analogs

8Br-dATP 8-Bromo-2-deoxy-adenosine-5-triphosphate, Sodium salt 8Br-cAMP 8-Bromo-Adenosine-3,5-cyclic monophosphate, Sodium salt 5I-UTP 5-Iodo-uridine-5-triphosphate, Sodium salt 5I-dUTP 5-Iodo-2-deoxy-uridine-5-triphosphate, Sodium salt 5Br-UTP 5-Bromo-uridine-5-triphosphate, Sodium salt 5Br-dUDP 5-Bromo-2-deoxy-uridine-5-diphosphate, Sodium salt 5Br-dUTP 5-Bromo-2-deoxy-uridine-5-triphosphate, Sodium salt 5I-dCTP 5-Iodo-2-deoxy-cytidine-5-triphosphate, Sodium salt
JBS Halo-ATP Kits

The kit contains all 12 halogenated Adenosine nucleotides (50 units* as lyophilized Na-salts) 2-Iodo-ADP, 2-Iodo-ATP, 2-Iodo-AppNHp (2-IodoAMPPNP) 2-Bromo-ADP, 2-Bromo-ATP, 2-Bromo-AppNHp (2-BromoAMPPNP) 8-Iodo-ADP, 8-Iodo-ATP, 8-Iodo-AppNHp (8-Iodo-AMPPNP) 8-Bromo-ADP, 8-Bromo-ATP, 8-Bromo-AppNHp (8-BromoAMPPNP)
JBS Halo-GTP Kits

The Kit contains all 6 halogenated Guanosine nucleotides (50 units* as lyophilized Na-salts) 8-Iodo-GDP, 8-Iodo-GTP, 8-Iodo-GppNHp (8-Iodo-GMPPNP) 8-Bromo-GDP, 8-Bromo-GTP, 8-Bromo-GppNHp (8-BromoGMPPNP) Product JBS Halo-ATP Kit JBS Halo-GTP Kit Qty 1 Kit 1 Kit
Halogenated oligonucleotides
Jena Bioscience is able to synthesize highly pure oligonucleotides containing halogenated bases. Please inquire.
Cat. No. PK-101 PK-103
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Crystallization Plates, Cover Slides, Tape and Grease

Crystallization plates Linbro plates
The standard 24-well Linbro plate for hanging drop crystallizations. CPL-104 Cat. No. CPL-101S CPL-101L Product Linbro Plate Linbro Plate Qty 10 50 CPL-105 Cat. No. CPL-102 Product
Consumables
Qty 20
CombiClover Plate includes 2 rolls of sealing tape, 2 and 4 wide Clover plate combo pack contains 10 CombiClover plates + 10 CompactClover plates plus 2 rolls of sealing tape, 2 and 4 wide CombiClover Junior Plate Junior plate includes one roll of 4 sealing tape CompactClover Plate includes 2 rolls of sealing tape, 2 and 4 wide CompactClover Junior Plate contains 10 CombiClover plates + 10 CompactClover plates plus 2 rolls of sealing tape, 2 and 4 wide
10+10
16
Clover Plates by Emerald BioStructures First Generation CombiClover Plates

Central reservoir is connected to four satellite drop chambers via dedicated vapor diffusion channels, forming a novel combinatorial crystallization clover. 24 crystallization clovers provide 96 fl at bottom sitting drop chambers that are totally clear and free of any cold weld or molding ejection pin marks. Open design of the combinatorial crystallization clover allows the simultaneous crystallization screening of four different macromolecule samples, while reducing by 75 % the amount of crystallization reagents, space and laboratory plastic ware used.
CPL-103
20
CPL-106
16
Manco Crystal Clear Sealing Tape

Cat. No. CTP-101S Product Manco Crystal Clear Sealing Tape, 2" wide Manco Crystal Clear Sealing Tape, 3" wide Manco Crystal Clear Sealing Tape, 4" wide Qty
60 m
CombiClover Junior Plate

Use less protein and crystallant per experiment. Cover the bottom of the sitting drop well with as little as 1l of solution. Utilizing the patented sitting drop well design and vapor diffusion channel. Central reservoir is connected to four satellite drop chambers via vapor diffusion channels, forming a novel combinatorial crystallization clover. 24 crystallization clovers provide 96 fl at-bottom sitting drop chambers allowing for 4 different experiments with one crystallant per clover. Well Volume 6 l, Reservoir volume 500 l.
CTP-101M
50 m
CTP-101L
50 m
Bayer Silicon Grease

The ideal sealant for crystallization setups. Chemical and heat resistant. Medium viscosity. Cat. No. CGR-101 Product Bayer Silicone Grease Qty 1 Tube (35 g)
First Generation CompactClover Plates

A compact clover design creates four separate reservoir partitions that are each connected to satellite drop chambers via dedicated vapor diffusion channels. 24 compact clovers provide 96 fl at bottom sitting drop chambers (30 microliter capacity) that are totally clear and free of any cold weld or molding ejection pin marks. A single compact crystallization plate can accommodate 4 times the number of crystallization trials as compared to other conventional plates of similar size. Reservoir chambers accommodate 250 l of crystallant, and extensive testing has shown that crystallization rates are comparable to other sitting drop devices.
Standard cover slides

Circular cover slides, 22 mm diameter. Not siliconized! For hanging drop, sitting drop and sandwich drop experiments.
Siliconized cover slides

Circular cover slides, 22 mm diameter, with siliconized surface . For hanging drop, sitting drop and sandwich drop experiments. Cat. No. CSL-101S Product Standard Circular cover slides, 22 mm diameter, not siliconized Standard Circular cover slides, 22 mm diameter, not siliconized Circular cover slides, 22 mm diameter, siliconized Circular cover slides, 22 mm diameter, siliconized Qty
CompactClover Junior Plates

96- well plate incorporating patented sitting drop well design and vapour diffusion channel. Use less protein and crystallant per experiment. Cover the bottom of the sitting drop well with as little as 1l of solution Quick setup with multichannel pipette Use for MicroBatch setup Reservoir volume 300l, well volume 3 l.
100
CSL-101L
1000
CSL-102S
100
CSL-102L
1000
! T : 040-27017477 / 65916167
F : 040-27016168
81
Cryo Crystallization
JBScreen Cryo
JBScreen Cryo is a crystallization screen for proteins, peptides, nucleic acids and macromolecular complexes. It contains preformulated reagents suitable for screening cryo and crystallization conditions using just a single screen. Based on an extensive data base search [1], the most successful crystallization conditions employing sufficiently large concentrations of cryoprotectants and well suited buffers were chosen for the JBScreen Cryo kits. JBScreen Cryo has been designed for standard vapor diffusion setups to determine initial crystallization conditions. Crystals grown using JBScreen Cryo can be directly flash-cooled in liquid nitrogen. If crystals were grown in non-cryo conditions, simply transfer them into a similar composed solution of JBScreen Cryo and allow them to soak for a few seconds before flash-cooling them. Cat. No. CC-103 CC-104 CC-105 CC-106 CC-107 CC-201S CC-201L Product JBScreen Cryo 1 JBScreen Cryo 2 JBScreen Cryo 3 JBScreen Cryo 4 JBScreen Cryo 1 - 4 JBScreen Cryo HTS S(1.0 ml per well) JBScreen Cryo HTS L(1.7 ml per well) Qty 1 Kit 1 Kit 1 Kit 1 Kit 4 Kits 1 Block 1 Block
Cryo Crystallization
JBScreen Cryo Pro

JBScreen Cryo Pro is the most convenient tool on the market for producing effective cryoprotectants from your crystallization reservoir solution. The kit contains 12 different compounds, divided into sugar/ amino acid-based cryoprotectants, alcohol-based cryoprotectants, and an oil-based cryoprotectant. Cat. No. CC-102 Product JBScreen Cryo Pro Qty 1 Kit
Emerald Biosystems Cryo I + II

Sparse matrices for the crystallization of biological macromolecules. Every formulation will flash-freeze to a clear amorphous glass in liquid nitrogen or in the cryo-stream at 100 K. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Crystals can be frozen directly from their growth chambers, thus avoiding the additional step of pre-equilibration with an artificial cryo solvent that can damage the crystal. Cat. No. EBS-CRYO-I EBS-CRYO-II EBS-CRYO-F EBS-BCY Product Cryo I Cryo II Cryo I + II Cryo I + II 96 well Matrix Block Qty 1 Kit 1 Kit 1 + 1 Kit
1 kit
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F : 040-27016168
Substrates, Reagents and Assay Kits for Analyzing MMPs

Chromogenic/fluorogenic substrates and antibodies for matrix metalloproteinases (MMPs)
Protease MMP-1 Chromogenic/ Fluorogenic Substrates 27082, 27084, 27086, 27088, 60571, 60572, 60576, 60578, 60579, 60581, 60582 27076, 27090, 27094, 27096, 60569, 60570, 60571, 60572, 60576, 60578, 60579, 60581, 60582 27098, 27100, 60579, 60580, 60581 27076, 27102, 27104 27106 27088, 27094, 27096, 60570, 60581 60569, 60570, 60571, 60572, 60574, 60575, 60576, 60578, 60579, 60580, 60581, 60582 27108 Antibodies Assay Kits
MMPs
Enzyme Detection Reagents and Assay kits
EnzoLyte 520 MMP-2 Assay Kit *Fluorimetric*

EnzoLyte 520 MMP-2 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of MMP-2 activities. Upon the cleavage of the FRET peptide by MMP-2, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Cat. No. 71151 Product EnzoLyte 520 MMP-2 Assay Kit *Fluorimetric* Qty 100 assays
29574
71128, 71150
MMP-2
29575
71129, 71151
MMP-3 MMP-7 MMP-8 MMP-9 MMP-12
29576 29577 29578 29579
71130, 71152 71132, 71153 71133, 71154 71134, 71155
Contents: 5-FAM/QXL520 FRET Peptide substrate for MMP-2, Assay buffer, Fluorescence reference standard for calibration, A detailed protocol.

The EnzoLyte 490 MMP-3 Assay Kit is a complete assay system designed to continuously analyze MMP activities or to screen MMP-3 inhibitors using a fluorogenic MMP-3 substrate. Cat. No. Product EnzoLyte 490 MMP-3 Assay Kit *Fluorimetric* Qty 500 assays
71137, 71157 71135, 71156
71130
MMP-13

The EnzoLyte 490 MMP-1 Assay Kit uses an optimized fluorogenic peptide substrate for continuous measurement of MMP-1 activities. Cat. No. 71128 Product EnzoLyte 490 MMP-1 Assay Kit *Fluorimetric* Qty 500 assays
Contents: EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nm upon cleavage), Assay buffer, Fluorescence reference standard for calibration, A detailed protocol

EnzoLyte 520 MMP-3 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-3 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13 and 14. Upon the cleavage of the FRET peptide by MMP-3, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Cat. No. 71152 Product EnzoLyte 520 MMP-3 Assay Kit *Fluorimetric* Qty 100 assays

EnzoLyte 520 MMP-1 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of the MMP-1 activities. Upon the cleavage of the FRET peptide by MMP-1, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Cat. No. 71150 Product EnzoLyte 520 MMP-1 Assay Kit *Fluorimetric* Qty 100 assays
Contents: 5-FAM/QXL520 FRET Peptide substrate for MMP-3, Assay buffer, Fluorescence reference, standard for calibration, A detailed protocol

The EnzoLyte 490 MMP-7 Assay Kit is a complete assay system designed to continuously analyze MMP activities or to screen MMP-7 inhibitors by using a fluorogenic MMP-7 substrate. The assays are performed in a convenient 96-well or 384-well microplate format. Cat. No. 71132 Product EnzoLyte 490 MMP-7 Assay Kit *Fluorimetric* Qty 500 assays
Contents: 5-FAM/QXL520 FRET Peptide substrate for MMP-1, Assay buffer, Fluorescence reference standard for calibration, A detailed protocol

The EnzoLyte 490 MMP-2 Assay Kit is a complete assay system designed to continuously analyze MMP activities or to screen MMP-2 inhibitors. Cat. No. 71129 Product EnzoLyte 490 MMP-2 Assay Kit *Fluorimetric* Qty 500 assays
! T : 040-27017477 / 65916167
F : 040-27016168
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Assay Kits for Analyzing MMPs

EnzoLyte 520 MMP-7 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520. Upon the cleavage of the FRET peptide by MMP-7, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Cat. No. 71153 Product EnzoLyte 520 MMP-7 Assay Kit *Fluorimetric* Qty 100 assays
MMPs

The EnzoLyte 490 MMP-12 Assay Kit is a complete assay system designed to continuously analyze MMP-12 activities or to screen for MMP-12 inhibitors by using a fluorogenic MMP-12 substrate. Cat. No. 71137 Product EnzoLyte 490 MMP-12 Assay Kit *Fluorimetric* Qty 500 assays
Contents: 5-FAM/QXL520 FRET Peptide substrate for MMP-7, Assay buffer, Fluorescence reference, standard for calibration

EnzoLyte 520 MMP-12 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-12 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520. Upon the cleavage of the FRET peptide by MMP-12, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Cat. No. 71157 Product EnzoLyte 520 MMP-12 Assay Kit *Fluorimetric* Qty 100 assays

EnzoLyte 520 MMP-8 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520. Upon the cleavage of the FRET peptide by MMP-8, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Cat. No. 71154 Product EnzoLyte 520 MMP-8 Assay Kit *Fluorimetric* Qty 100 assays
Contents: 5-FAM/QXL520 FRET Peptide substrate for MMP-8, Assay bufferFluorescence reference standard for calibration

The EnzoLyte 490 MMP-9 Assay Kit is a complete assay system designed to continuously analyze MMP activities or to screen for MMP-9 inhibitors using a fluorogenic MMP-9 substrate. Cat. No. 71134 Product EnzoLyte 490 MMP-9 Assay Kit *Fluorimetric* Qty 500 assays

The EnzoLyte 490 MMP-13 Assay Kit is a complete assay system designed to continuously analyze MMP-13 activities or to screen MMP-13 inhibitors by using a fluorogenic MMP-13 substrate. Cat. No. 71135 Product EnzoLyte 490 MMP-13 Assay Kit *Fluorimetric* Qty 500 assays

EnzoLyte 520 MMP-9 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRETpeptide, the fluorescence of 5-FAM is quenched by QXL520. Upon the cleavage of the FRET peptide by MMP-9, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Cat. No. 71155 Product EnzoLyte 520 MMP-9 Assay Kit *Fluorimetric* Qty 100 assays

EnzoLyte 520 MMP-13 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-13 with minimal cross-reaction with MMP-1, 2, 3, 7, 8, 9, 12, and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520. Upon the cleavage of the FRET peptide by MMP-13, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. Cat. No. 71156 Product EnzoLyte 520 MMP-13 Assay Kit *Fluorimetric* Qty 100 assays
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Chromogenic & Flurogenic Substrates for Detecting MMPs

Substrates for MMPs
Cat. No. 85111 85102 85112 85113 85103 85145 27078 27074 27082 27084 27086 Product Collagen (Type I), FITC conjugated *Water Soluble* for MMP-1 Collagen (Type I), FAM conjugated *Water Insoluble* for MMP-1, slow digesting & specific Collagen (Type IV), FAM conjugated for MMP-2, other Geloatinases & Collagenases. Elastin, FAM Conjugated For elastase & other proteases Elastin, FAM Conjugated *Water Insoluble*, slow digesting & specific Gelatin, FAM Conjugated For gelatinases & collagenases MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-OH Generic substrate for all MMPs DNP-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 Generic substrate for all MMPs MMP-1 Substrate I [DNP-Pro-Leu-Ala-Leu-Trp-Ala-Arg-OH] MMP-1 Substrate II [DNP-Pro-Leu-Gly-Cys(Me)-His-Ala-D-Arg-NH2] MMP-1 Substrate III [DNP-Pro-Cha-Abu-Cys(Me)-His-Ala-Lys (Abz(N-Me))-NH2] MMP-1/MMP-9 Substrate [DNP-Pro-Cha-Gly-Cys(Me)-His-Ala-Lys (Abz(N-Me))-NH2] MMP-7/8/13 Substrate I [MCA-Pro-Leu-Ala-Nva-Dpa-Ala-Arg-NH2] MMP-7/8/13 Substrate II [MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2] MMP-2/MMP-9 Substrate I [DNP-Pro-Leu-Gly-Met-Trp-Ser-Arg-OH] MMP-2/MMP-9 Substrate II [Ac-Pro-Leu-Gly-Mmp-Leu-Gly-OC2H5] MMP-3 Substrate I [DNP-Pro-Tyr-Ala-Tyr-Trp-Met-Arg-OH] MMP-3 Substrate II [NBD-Arg-Pro-Lys-Pro-Leu-Ala-Nva-Trp-Lys(DMC)NH2] Can also be an effective substrate for MMP-1, MMP-7, MMP-12 and MMP-13. MMP-7 Substrate I [DNP-Arg-Pro-Leu-Ala-Leu-Trp-Arg-Ser-OH] MMP-7 Substrate II [DABCYL-Arg-Pro-Leu-Ala-Leu-Trp-Arg-Ser-EDANS] MMP-8 Substrate [DNP-Pro-Leu-Ala-Tyr-Trp-Ala-Arg-OH] MMP-13 Substrate [MCA-Pro-Cha-Gly-Nva-His-Ala-Dpa- NH2] Inhibited by the tissue inhibitors of metalloproteinases, TIMP-1, TIMP-2 and TIMP-3. 27110 NFF-2 [MCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys (DNP)-NH2] NFF-3 [MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys (DNP)-NH2] TNO113 [DABCYL-GABA-Pro-Cha-Abu-Smc-His-Ala-Glu (EDANS)-Ala-Lys-NH2] TNO211 [DABCYL-g-Abu -Pro-Gln-Gly-Leu-Glu(EDANS)Ala-Lys-NH2] Qty 1 mg 5 mg 1 mg 1 mg 1 mg 5 mg 1 mg 1 mg 1 mg 60581-01 1 mg 1 mg 60575-01 1 mg 60569-01 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 60568-01 60573-01 60574-01 60582-01 1 mg 1 mg 60585-01 1 mg 1 mg 1 mg 1 mg 60576-01 60577-01 60570-01 60578-01 60571-01 60572-01 60583-01 60580-01 Cat. No. 60579-01 Product
MMPs
FRET based MMP substrates

These are based on the FAM/QXL 520 FRET. MMP recognized MMP-1, 2, 12, and 13 MMP-3, MMP-12 Qty 0.1 mg
5-FAM-Arg-Pro-Lys-Pro-TyrAla-Nva-Trp-Met-Lys(QXLTM 520)-NH2 5-FAM-Arg-Pro-Lys-Pro-ValGlu-Nva-Trp-Arg-Lys (QXLTM 520)-NH2 5-FAM-Pro-Cha-Gly-Nva-HisAla-Dap(QXLTM 520)-NH2 5-FAM-Pro-Leu-Ala-Nva-Dap (QXL TM 520)-Ala-Arg-NH2 5-FAM-Pro-Leu-Gly-Leu-Dap (QXL 520)-Ala-Arg-NH2 QXL 520-Arg-Pro-LysPro-Gln-Gln-Phe-Trp-Lys (5-FAM)-NH2 QXL 520-?-Abu-Pro-ChaAbu-Smc-His-Ala-Dab (5-FAM)-Ala-Lys-NH2 (Smc=S-Methyl-L-cysteine) QXL 520-Arg-Pro-Lys-ProLeu-Ala-Nva-Trp-Lys (5-FAM)-NH2 QXL 520-Pro-Leu-Ala-LeuTrp-Ala-Arg-Lys(5-FAM)-NH2 QXL 520 -Pro-Leu-Ala-TyrTrp-Ala-Arg-Lys(5-FAM)-NH2 QXL 520-Pro-Leu-Gly-Cys (Me)-His-Ala-D-Arg-Lys (5-FAM)-NH2 QXL 520-Pro-Leu-Gly-LeuTrp-Ala-D-Arg-Lys(5-FAM)-NH2 QXL 520-Pro-Leu-Gly-MetTrp-Ser-Arg-Lys(5-FAM)-NH2 QXL 520-Pro-Tyr-Ala-TyrTrp-Met-Arg-Lys(5-FAM)-NH2 QXL 520-?-Abu-Pro-Gln-GlyLeu-Dab(5-FAM)-Ala-Lys-NH2 QXL 520-Arg-Pro-Leu-AlaLeu-Trp-Arg-Lys(5-FAM)-NH2 QXL 570-Lys-Pro-Leu-AlaNva-Dap(5-TAMRA)-Ala-Arg-NH2
0.1 mg
MMP-3, MMP-12 MMP-1, 2, 7, 8, 12, and 13 MMP-3, MMP-12 MMP-12, MMP-13
0.1 mg 0.1 mg 0.1 mg 0.1 mg
MMP-1, 2, 3, 7, 8, 9, 12 and 13
0.1 mg
MMP-7, MMP-12, MMP-13 MMP-1, 7, 8, 12, and 13 MMP-13 MMP-1, 2, 8, 9, 12, and 13
0.1 mg
27088
0.1 mg 0.1 mg 0.1 mg
27090 27076 27094 27096 27098 27100
MMP-13 MMP-2, MMP-13 MMP-7, MMP-12, MMP-13 MMP-1, 2, 7, 8, 12 and 13 MMP-1, 2, 7, 8, 12 and 13 Generic MMP substrate
0.1 mg 0.1 mg 0.1 mg 0.1 mg 0.1 mg 0.1 mg
27102 27104 27106 27108
MMP Antibodies
Western blot and immunohistochemistry Cat. No. 29574 29575 Product Anti-MMP 1 Anti-MMP 2 Anti-MMP 3 Anti-MMP 7 Anti-MMP 8 Anti-MMP 9 Anti-MMP 10 Anti-MMP 16 Qty 0.1 mg 0.1 mg 0.1 mg 0.1 mg 0.1 mg 0.1 mg 0.1 mg 0.1 mg
27114
1 mg
29576 29577
27061
1 mg
29578 29579
25350
1 mg
29580 29581
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Reagents and Assay Kits for Analyzing Viral Proteases

EnzoLyte 490 HCV Protease Assay Kit *Fluorimetric*
The EnzoLyte 490 HCV Protease Assay Kit uses an optimized FRET peptide substrate for the continuous measurement of HCV NS3/4a activity. This FRET substrate is cleaved specifically by HCV NS3/4a protease thereby liberating the C-terminal peptide-fluorophore fragment from the proximity quenching effect of the dark quencher, resulting in a more than 10-fold increase in fluorescence. Cat. No. 71126 Product EnzoLyte 490 HCV Protease Assay Kit *Fluorimetric* Qty 500 assays Cat. No. 71127 Product
VIRAL PROTEASES
EnzoLyte 490 *Fluorimetric*
HIV
Protease
Assay
Kit
The EnzoLyte 490 HIV Protease Assay Kit uses an optimized FRET peptide substrate for a continuous measurement of HIV protease activities. This FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of the recombinant HIV-1 PR with this substrate results in specific cleavage and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. Qty 500 assays
Contents: EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nm upon cleavage), Assay buffer, Fluorescence reference standard for calibration
EnzoLyte 490 HIV Protease Assay Kit *Fluorimetric*
Contents: EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nm upon cleavage), Assay buffer, HIV protease inhibitor, Fluorescence reference standard for calibration, A detailed protocol
The EnzoLyte 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. Cat. No. 71145 Product EnzoLyte 520 HCV Protease Assay Kit *Fluorimetric* Qty 100 assays
EnzoLyte 520 *Fluorimetric*
HIV
Protease
Assay
Kit
The EnzoLyte 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL 520 (quencher) for continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/ 520 nm. Cat. No. 71147 Product EnzoLyte 520 HIV Protease Assay Kit *Fluorimetric* Qty 100 assays
Contents: 5-FAM/QXL 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage), Fluorescence reference standard for calibration

The EnzoLyte 620 HCV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor TR (fluorophore) and QXL 610 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of Hilyte Fluor TR is quenched by QXL 610. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of Hilyte Fluor TR is recovered and can be continuously monitored at excitation/emission = 590 nm/620 nm. Cat. No. 71146 Product EnzoLyte 620 HCV Protease Assay Kit *Fluorimetric* Qty 100 assays
Contents: 5-FAM/QXL 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage), Assay buffer, HIV protease inhibitor, Fluorescence reference standard for calibration, A detailed protocol
EnzoLyte 620 HIV Protease Assay Kit *Fluorimetric*

EnzoLyte 620 HCV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor TR (fluorophore) and QXL 610 (quencher) for continuous measurement of the enzyme activities. In the intact FRET peptide, the fluorescence of Hylite Fluor TR is quenched by QXL 610. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of Hylite Fluor TR is recovered and can be continuously monitored at excitation/emission = 590 nm/620 nm. Cat. No. 71148 Product EnzoLyte 620 HIV Protease Assay Kit *Fluorimetric* Qty 100 assays
Contents: HiLyte Fluor TR/QXL 610-based FRET peptide substrate (Ex/ Em=590/620 nm upon cleavage), Assay buffer, Fluorescence reference standard for calibration
HCV Protease FRET Substrate I

This is a HCV protease substrate incorporating an ester bond between residues P1 and P1. It is widely used for continuous assay of NS3 protease activity. Cat. No. 22991-025 Product HCV Protease FRET Substrate I [Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu --(COO)Ala-Ser-Lys(DABCYL)-NH2] Qty 0.25 mg
Contents: FRET peptide substrate (fluorogenic indicator, Ex/Em=590/610nm upon cleavage), Assay buffer, HIV protease inhibitor, Fluorescence reference standard for calibration, A detailed protocol
HIV Protease FRET Substrate I

Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. The fluorescence quantum yields of the HIV-1 PR substrate in the FRET assay increased by 40.0- and 34.4-fold, respectively, per mole of substrate cleaved. Cat. No. 22992 Product HIV Protease FRET Substrate I Qty 1 mg
This is an internally quenched fluorescent substrate cleaved specifically between the Ala-Ser peptide bond thereby liberating the C-terminal peptideEDANS fragment from the proximity quenching effect of DABCYL group. Cat. No. 22998 Product CMV Protease FRET Substrate I [DABCYL-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser -Arg-Leu-Ala-EDANS] Qty 1 mg
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F : 040-27016168
Reagents and Assay Kits for Analyzing Miscellaneous Proteases

EnzoLyte Green Protease Assay Kit *Fluorimetric*
Our EnzoLyte Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugates fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dyelabeled peptides. The EnzoLyte protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases. Cat. No. 71124 Product EnzoLyte Green Protease Assay Kit *Fluorimetric* Qty 500 assays
PROTEASES
TACE FRET Substrate I

TACE FRET substrate I is derived from the cleavage sequence of proTNFa recognized by TNFa-cleaving enzyme (TACE). Incubation of the TACE substrate with recombinant human TACE gives a specific cleavage to restore the quenched fluorescence. The substrate is widely used to screen inhibitors of TNF-convertase. Cat. No. 25043 Product TACE FRET Substrate I (DABCYL-Leu-Ala-Gln-Ala-Val-Arg-SerSer-Ser-Arg-EDANS) Qty 1 mg
Substrate for Calpain

The colorless and nonfluorescent (Z-Ala-Ala) 2Rh110 is hydrolyzed to highly fluorescent rhodamine 110. Alternatively, (Z-Ala-Ala) 2Rh110 can also be used to detect calpain in a chromogenic mode since the enzymatic product (rhodamine 110) exhibits a large extinction coefficient. Cat. No. 60320-5 Product (Z-Ala-Ala)2Rh110 [Rhodamine 110, bis-(CBZ-L-alanylL-alanine amide)] Qty 5 mg
Contents: Fluorescently labeled casein with high ratio of dye/protein, Trypsin (positive control), Assay buffer, A detailed protocol
EnzoLyte Red Protease Assay Kit *Fluorimetric*

Same as above, except that it uses Rhodamine. Cat. No. 71140 Product EnzoLyte Red Protease Assay Kit *Fluorimetric* Qty 500 assays
Contents: Fluorescently labeled casein with high ratio of dye/protein (Ex/Em=546/ 575nm on cleavage), Trypsin (positive control), Assay buffer
Substrate for Elastase

The colorless and nonfluorescent (Z-Ala-Ala-Ala-Ala) 2Rh110 is hydrolyzed to highly fluorescent rhodamine 110. Cat. No. 60321-5 Product (Z-Ala-Ala-Ala-Ala) 2Rh110 [Rhodamine 110, bis-(CBZ-L-alanylL-alanyl-L-alanyl-L- alanine amide)] Qty 5 mg
Substrates for Proteases Casein, FITC/TAMRA Conjugated

Cat. No. 85100 85101 Product Casein, FITC Conjugated Casein, TAMRA Conjugated Qty 5 mg 5 mg
Substrate for Elastase/Trypsin

The colorless and nonfluorescent (Z-Ala-Arg) 2Rh110 is hydrolyzed to highly fluorescent rhodamine 110. Cat. No. Product (Z-Ala-Arg)2Rh110 [Rhodamine 110, bis-(CBZ-L-alanyl-L -arginine amide)] Qty 5 mg
Substrate for Trypsin/Cathepsin B & L

60322-5 The colorless (Z-Arg) 2Rh110 is hydrolyzed to highly fluorescent rhodamine 110. Cat. No. 60323-5 Product (Z-Arg)2Rh110 [Rhodamine 110, bis(CBZ-L-arginine amide)] Qty 5 mg
Malaria Aspartyl Proteinase FRET Substrate I

A useful fluorogenic substrate for the continuous assay of malaria aspartyl proteinase. Cat. No. 24480 Product Malaria Aspartyl Proteinase FRET Substrate I [DABCYL-Glu-Arg-NlePhe-Leu-Ser-Phe-Pro-EDANS] Qty 1 mg
Renin FRET Substrate I

This substrate contains a Leu-Val renin cleavage site that occurs in the N-terminal peptide of human angiotensinogen. Cleavage of the substrate by renin liberates the peptidyl-EDANS fragment from proximity with the DABCYL acceptor, restoring the fluorescence of the EDANS moiety. Cat. No. 24478 Product Renin FRET Substrate I (DABCYL-g-Abu-Ile-His-Pro-Phe-HisLeu-Val-Ile-His-Thr-EDANS) Qty 1 mg
! T : 040-27017477 / 65916167
F : 040-27016168
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Chromogenic & Fluorogenic Substrates for Detecting Glycosidases
GLYCOSIDASES
Cat. No. 85624
Product 5-Bromo-4-chloro-3-indoxyl-N-acetyl --D-N-acetylneuraminic acid, sodium salt Chromogenic neuraminidase substrate used in histochemistry abs: ~610 nm; em: N/D CUG [3-Carboxyumbelliferyl--D-galactopyranoside] Excellent fluorogenic substrate for detecting -galactosidase abs: 386 nm; em: 448 nm FDGlcU [Fluorescein di--D-glucuronide] The most sensitive fluorogenic substrate for detecting -glucuronidase abs: 490 nm; em: 514 nm 4-Methylumbelliferyl--D-glucoside Excellent fluorogenic substrate for detecting -glucosidase abs: 360 nm; em: 499 nm MUG [4-Methylumbelliferyl--D-galactopyranoside] Excellent fluorogenic substrate for detecting -galactosidase abs: 360 nm; em: 499 nm MUGlcU [4-Methylumbelliferyl--D-glucuronide] Excellent fluorogenic substrate for detecting -galactosidase abs: 360 nm; em: 499 nm 4-Nitrophenyl-N-acetyl-b-D-galactosaminide Chromogenic N-acetylgalactosaminidase substrate, abs: 399 nm 4-Nitrophenyl-N-acetyl-b-D-glucosaminide Chromogenic N-acetylglucosaminidase substrate, abs: 399 nm; em: N/A 4-Nitrophenyl--D-cellobioside Chromogenic cellobiohydralase (e.g. cellobiosidase) substrate used in histochemistry abs: 399 nm; em: N/A 4-Nitrophenyl--D-fucopyranoside Chromogenic substrate for -fucosidase used in histochemistry abs: 399 nm; em: N/A 4-Nitrophenyl--D-galactopyranoside Excellent chromogenic -galactosidase substrate abs: 399 nm; em: N/A 4-Nitrophenyl--D-galactopyranoside Excellent chromogenic -galactosidase substrate abs: 399 nm; em: N/A 4-Nitrophenyl--D-glucopyranoside Excellent chromogenic -galactosidase substrate abs: 399 nm; em: N/A 4-Nitrophenyl--D-glucopyranoside Excellent chromogenic -galactosidase substrate abs: 399 nm; em: N/A X--xyloside [5-Bromo-4-chloro-3-indoxyl--Dxylopyranoside] Chromogenic -xylosidase substrate used in histochemistry abs: ~610 nm; em: N/D
Qty 5 mg
Cat. No. 85640
Product 4-Nitrophenyl--D-glucuronic acid, sodium salt chromogenic galactosidase substrate abs: 399 nm; em: N/A 4-Nitrophenyl--D-mannopyranoside chromogenic -galactosidase substrate abs: 399 nm; em: N/A -Trifluoromethylumbelliferyl--Dgalactopyranoside chromogenic -galactosidase substrate abs: 385 nm; em: 502 nm -Trifluoromethylumbelliferyl-D-glucuronide fluorogenic glucuronidase substrate abs: 385 nm; em: 502 nm X-cellobioside [5-Bromo-4-chloro-3-indoxyl-Nacetyl--D-cellobioside] Chromogenic cellobiohydralase (e.g. cellobiosidase) substrate used in histochemistry abs: ~610 nm; em: N/D X-fucoside [5-Bromo-4-chloro-3-indoxyl-D-fucopyranoside] Chromogenic substrate for -fucosidase used in histochemistry abs: ~610 nm; em: N/D X-a-Gal [5-Bromo-4-chloro-3-indolyl-D-galactopyranoside] Excellent chromogenic -galactosidase substrate used in histochemistry abs: ~610 nm; em: N/D X-Gal [5-Bromo-4-chloro-3-indolyl-D-galactopyranoside] Excellent chromogenic -galactosidase substrate used in histochemistry abs: ~610 nm; em: N/D X-GalNAc [5-Bromo-4-chloro-3-indoxylN-acetyl--D-galactosaminide] Chromogenic N-acetylgalactosaminidase substrate used in histochemistry abs: ~610 nm; em: N/D X-GluNAc [5-Bromo-4-chloro-3-indoxyl-N-acetyl-D-glucosaminide] Chromogenic Nacetylgalactosaminidase substrate used in histochemistry abs: ~610 nm; em: N/D X-GlcU [5-Bromo-4-chloro-3-indoxyl-D-glucuronic acid, sodium salt] Excellent chromogenic -glucuronidase substrate used in histochemistry abs: ~610 nm; em: N/D X--Glu [5-Bromo-4-chloro-3-indoxyl--Dglucopyranoside] Excellent chromogenic -galactosidase substrate used in histochemistry abs: ~610 nm; em: N/D X--D-mannoside [5-Bromo-4-chloro-3-indoxyl--Dmannopyranoside] Chromogenic -manosidase substrate used in histochemistry abs: ~610 nm; em: N/D
Qty 100 mg
85641 10 mg 85615
100 mg
85601
100 mg
85602
5 mg
85616
25 mg
85625 250 mg
100 mg
85607
85605
250 mg
85626
25 mg
85606
100 mg
85627
25 mg
85618
100 mg
85620
1g
85619
100 mg
85634
100 mg
85622
25 mg
85637
100 mg
85623
25 mg
85638
1g 85630 1g
25 mg
85636
85639
1g
85628
25 mg
85629
1g 85632 25 mg
100 mg
85635
88
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F : 040-27016168
Reagents and Assay kits for Phosphatases, Phospholipases and Galactosidases
PHOSPHATASES/PHOSPHOLIPASES/ GALACTOSIDASES
EnzoLyte FDP Alkaline Phosphatase Assay Kit *Fluorimetric*
Other Chromogenic and Fluorogenic Substrates for Detecting Phosphatases and Phospholipases
Cat. No. 85633 Product BCIP *UltraPure Grade* [5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt] abs: ~610 nm; em: N/A pNPP [4-Nitrophenyl phosphate, disodium salt] *UltraPure Grade* Excellent chromogenic phosphatase substrate, abs: 399 nm MUP *UltraPure Grade*4Methylumbelliferyl phosphate, free acid] Abs (max): 360 nm; em: 499 nm MUP, DCA *UltraPure Grade*4Methylumbelliferyl phosphate, dicyclohexylammonium salt, trihydrate] abs: 360 nm; em: 499 nm MUP, DSS *UltraPure Grade* [4-Methylumbelliferyl phosphate, disodium salt] abs: 360 nm; em: 499 nm X-InP [5-Bromo-4-chloro-3-indoxyl myo-inositol-1-phosphate, ammonium salt] Qty 100 mg
The EnzoLyte FDP Alkaline Phosphatase Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). Cat. No. 71109 Product EnzoLyte FDP Alkaline Phosphatase Assay Kit *Fluorimetric* Qty 500 assays
85301
1g
Contents: FDP phosphatase substrate (Ex/Em=48520/52820 nm upon dephosphorylation), Calibration standard, Reaction buffer, Stop buffer.
EnzoLyte FDP Alkaline Phosphatase ELISA Assay Kit *Fluorimetric*

The EnzoLyte FDP Alkaline Phosphatase ELISA Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in ELISA.
85310
1g
85311 Cat. No. 71101-R Product EnzoLyte FDP Alkaline Phosphatase ELISA Assay Kit *Fluorimetric* Qty 500 assays 85312
1g
Contents: FDP phosphatase substrate (Ex/Em=48520/52820 nm upon dephosphorylation), Assay buffer, Stop solution, Wash buffer, Alkaline phosphataseconjugated secondary antibody (goat anti-rabbit IgG), An optimized ELISA protocol
1g
85631
5 mg
Fluorescein diphosphate, tetraammonium salt (FDP)

Please view page#
FDG [Fluorescein di- -D-galactopyranoside]

Fluorescein di- -D-galactopyranoside (FDG) is one of the most sensitive fluorogenic substrates available for detecting -galactosidase. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. FDG has been widely used for identifying lacZpositive cells with fluorescence microscopy and flow cytometry.
EnzoLyte pNPP Alkaline Phosphatase ELISA Assay Kit *Colorimetric*

The EnzoLyte pNPP Alkaline Phosphatase ELISA Assay Kit provides optimized pNPP assay buffer, modified ELISA wash buffer and AP-conjugated secondary antibody. The assay is highly sensitive and has a large dynamic range. Cat. No. 71232-M Product EnzoLyte pNPP Alkaline Phosphatase ELISA Assay Kit *Colorimetric* Qty 500 assays
Resorufin -D-Galactopyranoside
Unlike FDG that requires a two-step hydrolysis to generate maximum fluorescence, resorufin -D-galactopyranoside requires only a singlestep hydrolysis reaction to attain full fluorescence. Resorufin galactoside has also been used to quantitate -galactosidase activity in single yeast cells by flow cytometry and to detect immobilized -galactosidase activity. Cat. No. 85600 Product FDG [Fluorescein di--D-galactopyranoside] Resorufin -D-Galactopyranoside Qty 5 mg
EnzoLyte pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit *Colorimetric*
EnzoLyte TM pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a sensitive colorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form. Cat. No. 71233 Product EnzoLyte pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit *Colorimetric* Qty 500 assays
85617
25 mg
Contents: pNPP chromogenic substrate (absorption max = 405 nm on dephosphorylation), Assay buffer, Stop solution.
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Reagents and Assay Kits for Analyzing Secretases

EnzoLyte 520 -Secretase Assay Kit
EnzoLyte -Secretase Assay Kit provides a sensitive and efficient method for screening potential b -secretase inhibitors. This kit uses a -secretasecleavable FRET peptide substrate. The specific cleavage of the peptide by -secretase physically separates the donor and acceptor, recovering the quenched fluorescence that is directly related to -secretase activity. Cat. No. 71144 Product EnzoLyte 520 -Secretase Assay Kit Qty 100 assays
-Secretase
-Secretase Substrate III

This is a FRET-based b-secretase substrate that is cleaved by -secretase, thereby liberating the MCA fragment from the proximity quenching effect of the DNP group. This cleavage results in fluorescence enhancement that is observed at the MCA emission wavelength of ~390 nm. Cat. No. 60270 Product -Secretase Substrate III [MCA-Glu-Val-Asn-Leu-Asp-Ala-GluPhe-Lys(DNP)-OH] Qty 1 mg
Contents: FRET peptide substrate (fluorogenic indicator, Ex/Em = 488/520 nm upon cleavage), -Secretase (positive control), Assay buffer, Fluorescence reference standard for calibration, A detailed protocol
-Secretase Substrate V
This is a FRET-based -secretase substrate that is cleaved by -secretase, thereby liberating the HiLyte Fluor 488 fragment from the proximity quenching effect of the QXL 520 group. This results in fluorescence enhancement that is observed at the HiLyte Fluor emission wavelength of ~520 nm with excitation at 488 nm.
-Secretase Substrate I
This is FRET-based -secretase substrate that is cleaved by -secretase, thereby liberating the MCA fragment from the proximity quenching effect of the DNP group. This cleavage results in fluorescence enhancement that is observed at the MCA emission wavelength of ~390 nm. Cat. No. 60271 Product -Secretase Substrate I [MCA-His-Gln-Lys-Leu-Val-PhePhe-Ala-Lys(DNP)-OH] Qty 1 mg
Cat. No. 60604-01
Product -Secretase Substrate V [HiLyte Fluor 488-Glu-Val-AsnLeu-Asp-Ala-Glu-Phe-Lys(QXL 520)-OH]
Qty 1 mg
-Secretase Substrate I
This substrate is cleaved by -secretase thereby liberating the MCA fragment from the proximity quenching effect of the DNP group. This results in fluorescence enhancement that is observed at the MCA emission wavelength of ~390 nm. Cat. No. 60268 Product -Secretase Substrate I [MCA-Glu-Val-Lys-Val-Asp-Ala-GluPhe-Lys(DNP)-OH] Qty 1 mg
-Secretase Substrate II
This is an internally quenched fluorescent -secretase substrate where the Val residue is replaced by Met. This substrate is cleaved by -secretase, thereby liberating the MCA fragment from the proximity quenching effect of the DNP group. This cleavage results in fluorescence enhancement that is observed at the MCA emission wavelength of ~390 nm. Cat. No. 60269 Product -Secretase Substrate II [MCA-Glu-Val-Lys-Met-Asp-AlaGlu-Phe-Lys(DNP)-OH] Qty 1 mg
90
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F : 040-27016168
Reagents & Assay kits for Peroxidases, Oxidases & Dehydrogenases

ADHP (10-Acetyl-3,7-dihydroxyphenoxazine)
This is not only a stable fluorogenic substrate for HRP but also an ultrasensitive probe for H2O2. In the presence of HRP and H 2O2, ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Cat. No. 85500 Product ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) Qty 25 mg
PEROXIDASES/REDOX ENZYMES
Other Chromogenic and Fluorogenic Sustrates for Detecting Peroxidases

Cat. No. 85501 Product ABTS [2,2'-Azino-bis(3-ethylbenzothiazoline6-sulfonic acid) diammonium salt], 1M solution in DMSO Peroxidase substrate suitable for use in ELISA procedures; produce a soluble green product; abs:405 nm; em: N/A AEC [3-Amino-9-Ethylcarbazole], 1M solution in DMSO produce an insoluble red product upon peroxidase/H2O2 oxidation abs: N/D; em: N/D 4-CN [4-Chloro-1-naphthol], 1M solution in DMSO produce an insoluble red product upon peroxidase/H2O2 oxidation abs: N/D; em: N/D DAB [3,3'-Diaminobenzidine tetrahydrochloride], 1M aqueous solution produce an insoluble brown product upon HRP/H2O2 oxidation abs: N/D; em: N/D HPPA [3-(4-Hydroxyphenyl)propionic acid]*UltraPure Grade* Fluorogenic peroxidase substrate abs: 330 nm; em: 410 nm Luminol *UltraPure Grade* Luminogenic peroxidase substrate; also used for detecting superoxide abs: 355 nm; em: 411 nm MCLA [2-Methyl-6-(4-methoxyphenyl)3,7-dihydroimidazo[1,2-a]pyrazin -3-one, hydrochloride] Mainly used for luminogenic detection of superoxide abs:430 nm; em: 546 nm NBD methylhydrazine [N-Methyl-4-hydrazino-7-nitrobenzofurazan] Flurogenic peroxidase substrate abs:493 nm; em: 552 nm OPD [o-Phenylenediamine dihydrochloride], 1M solution in DMSO Peroxidase substrate suitable for use in ELISA procedures; produce a soluble yellow-orange product abs: N/D; em: N/D TMB [3,3',5,5'-Tetramethylbenzidine dihydrochloride], 1M solution in DMSO Soluble peroxidase substrate; yields a blue soluble product with maximum absorption of 370 and 652 nm that changes to yellow upon addition of strong acid such as sulfuric acid abs:492 nm; em: N/D Qty 1 mL
EnzoLyte ADHP Hydrogen Peroxide Assay Kit *Fluorimetric*

EnzoLyte ADHP Hydrogen Peroxide Assay Kit uses highly purified ADHP to quantify hydrogen peroxide in solutions, in cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions.
85502
5 mL
85503 Cat. No. 71112 Product EnzoLyte ADHP Hydrogen Peroxide Assay Kit *Fluorimetric* Qty 500 assays 85504
5 mL
EnzoLyte ADHP Peroxidase Assay Kit *Fluorimetric*

EnzoLyte ADHP Peroxidase Assay Kit uses highly purified ADHP to quantify peroxidase activity in solutions, in cell extracts and in live cells and on solid surfaces (such as PVDF membranes).
5 mL
85505 Cat. No. 71111 Product EnzoLyte ADHP Peroxidase Assay Kit *Fluorimetric* Qty 500 assays
1g
82100
1g
Entoleted ADHP Peroxidase ELISA Assay Kit *Fluorimetric*

EnzoLyte ADHP Peroxidase ELISA Assay Kit uses highly purified ADHP to quantify HRP activity in the secondary antibody or streptavidin-HRP conjugates. Cat. No. 71110-R Product Entoleted ADHP Peroxidase ELISA Assay Kit *Fluorimetric* Qty 500 assays 85727 87350
25 mg
5 mg
EnzoLyte ADHP Peroxidase ELISA Assay Kit *Fluorimetric*

EnzoLyte ADHP Peroxidase ELISA Assay Kit uses highly purified ADHP to quantify HRP activity in the secondary antibody or streptavidin-HRP conjugates. Cat. No. 71110-M Product EnzoLyte ADHP Peroxidase ELISA Assay Kit *Fluorimetric* Qty 500 assays
85506
5 mL
85507
1 mL
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F : 040-27016168
91
Chromogenic and Flurogenic Substrates for Detecting Cytochrome P-450 and other Oxidases
Enzyme Substrates for Detecting Cytochrome P-450 and other Oxidases
Cat. No. 85726 Product Benzyloxyresorufin [Resorufin benzyl ether] Fluorogenic cytochrome P-450 substrate abs: 571 nm; em: 585 nm 5-(and-6)-Carboxy-2',7'dichlorodihydrofluorescein diacetate Cell-impermeable substrate of oxidases (including peroxidase) abs: 495 nm; em: 529 nm 5-Carboxy-2',7'dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) Cell-permeable substrate of oxidases (including peroxidase). abs: 495 nm; em: 529 nm 6-Carboxy-2',7'dichlorodihydrofluorescin diacetate, di(acetoxymethyl ester) Cell-permeable substrate of oxidases (including peroxidase). abs: 495 nm; em: 529 nm 3-Cyano-7-ethoxycoumarin Fluorogenic cytochrome P-450 substrate abs: 408 nm; em: 450 nm 2',7'-Dichlorodihydrofluorescein diacetate [2',7'-Dichlorofluorescin diacetate] Cell-permeable substrate of oxidases (including peroxidase) abs: 495 nm; em: 529 nm Dihydrocalcein, AM Generic substrate of oxidases (including peroxidase) abs: 494 nm; em: 517 nm Dihydrocalcein, AM *Custom Packaging*Generic substrate of oxidases (including peroxidase) abs: 494 nm; em: 517 nm Dihydroethidium [Hydroethidine] Generic substrate of oxidases (including peroxidase); The oxidized product tends to accumulate in nuclei. abs: 518 nm; em: 605 nm Dihydroethidium [Hydroethidine], 5 mM soln. in DMSO Generic substrate of oxidases (including peroxidase); The oxidized product tends to accumulate in nuclei. abs: 518 nm; em: 605 nm Dihydrofluorescein diacetate [H2FDA] Generic substrate of oxidases (including peroxidase) abs: 492 nm; em: 517 nm Dihydrorhodamine 123 Generic substrate of oxidases (including peroxidase) in mitochondria abs: 507 nm; em: 529 nm Qty 10 mg Cat. No. 85720 Product
CYTOCHROME P450 & OXIDASES

Qty 10 mg
Dihydrorhodamine 123 Generic redox indicator of oxidases (including peroxidase); The oxidized product tends to accumulate in mitochondria. abs: 507 nm; em: 529 nm Dihydrorhodamine 123, 5 mM solution in DMSO Generic redox indicator of oxidases (including peroxidase); The oxidized product tends to accumulate in mitochondria. abs: 507 nm; em: 529 nm Dihydrorhodamine 6G Generic substrate of oxidases (including peroxidase) in mitochondria abs: 528 nm; em: 551 nm Ethoxyresorufin [Resorufin ethyl ether] Fluorogenic cytochrome P-450 substrate that generates red fluorescent product upon enzyme cleavage abs: 571 nm; em: 585 nm 7-Ethoxy-4-trifluoromethylcoumarin Fluorogenic cytochrome P-450 substrate that generates blue/green fluorescent product upon enzyme cleavage abs: 385 nm; em: 502 nm Methoxyresorufin [Resorufin methyl ether] Fluorogenic cytochrome P-450 substrate that generates red fluorescent product upon enzyme cleavag abs: 571 nm; em: 585 nm MPTS [8-Methoxypyrene-1,3,6-trisulfonic acid, trisodium salt] Cell-impermeable cytochrome P-450 substrate that generates yellow fluorescent product upon enzyme cleavage abs: 454 nm; em: 511 nm Resazurin, sodium salt *UltraPure Grade* Widely used for measuring cell cytotoxicity, proliferation and mitochondrial metabolic activity in isolated neural tissue; also used for measuring dehydrogenase activity abs: 571 nm; em: 585 nm
85719 25 mg
1 mL
85703
85702
5 mg
85712
25 mg
85715 5 mg
5 mg
85708
85716 10 mg
25 mg
85705
85706
100 mg 85707 5 mg
85700
1 mg 85725
100 mg
85701
1 mg
85717
10 mg
85740
100 mg
85718
1 mL
Luciferin and Luciferases

Cat. No. 25 mg 82250 82252 10 mg Product D-Luciferin, free acid *UltraPure Grade* abs: 328 nm; em: 532 nm D-Luciferin, potassium salt *UltraPure Grade* abs: 328 nm; em: 532 nm D-Luciferin, sodium salt D-Luciferin, free acid *UltraPure Grade* abs: 328 nm; em: 532 nm Qty 25 mg 25 mg
85710
85711
82251 82251
25 mg 25 mg
92
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- Amyloid Peptides and Related Assay Reagents

DHL fluorescent -amyloid (1-28) sampler kit
Fluorophore labeled beta-amyloid peptides have been used in investigating beta-amyloids aggregation, generation and clearance, microglial activation and phagocytosis. DHL fluorescent b-amyloid (1-28) sampler kit provides eight fluorescent beta-amyloid (128) peptides and one unlabeled peptide as control. The sampler kit provides ample choices for researchers interested in beta-amyloid related experiments. Cat. No. 71020 Product DHL fluorescent -amyloid (1-28) sampler kit Qty 1 kit
AMYLOIDS
Assay kits and reagents
-Amyloid (1-42) Tracers -Amyloid (1-42), N-Terminal Labeled

DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Cat. No. 24224 Product -Amyloid (1-42), N-Terminal Unlabeled Also available in Biotin and fluor labeled form Qty 0.5 mg
Anti- -Amyloid antibodies

Cat. No. 28182 Product Rabbit Anti--Amyloid (anti aa 672-713), Sp:Human; used in immunoprecipitation immunofluorescence Also available in Biotin and fluor labeled form Anti--Amyloid (NT), monoclonal Species: Human; Host: Rabbit Also available in Biotin and fluor labeled form Qty 100 g
-Amyloid (1-28) Tracers -Amyloid (1-28), N-Terminal Labeled (Tag-DAEFRHDSGYEVHHQKLVFFAEDVGSNK)

Cat. No. 24231 Product -Amyloid (1-28), N-Terminal Unlabeled Also available in Biotin and fluor labeled form Qty
29547 0.5 mg
0.1 mg
DHL fluorescent -amyloid (1-40) sampler kit

Fluorophore labeled beta-amyloid peptides have been used in investigating beta-amyloids aggregation, generation and clearance, microglial activation and phagocytosis. DHL fluorescent b-amyloid (1-40) sampler kit provides eight fluorescent beta-amyloid (140) peptides and two unlabeled peptide as control. The sampler kit provides ample choices for researchers interested in beta-amyloid related experiments. Cat. No. 71021 Product DHL fluorescent -amyloid (1-40) sampler kit Qty 1 kit
Vital Stains for Amyloids

Cat. No. 88300 Product Qty
BSB [(trans,trans)-1-Bromo-2,5-bis-(35 mg hydroxycarbonyl-4-hydroxy)styrylbenzene] Congo Red derivative, binds to amyloid inclusions in situ and amyloid in brains of live animals. Used for monitoring amyloid fibrils assembled from the Ab peptide, -synuclein and tau. BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] uncharged derivative of thioflavin-T that has high a affinity for Ab fibrils and shows very good brain entry and clearance. BTA-2 [2-(4'-(dimethylamino)phenyl) -6-methyl-benzothiazole]Uncharges derivative of Thioflavin-T, stains both plaques and neurofibrillary tangles in post mortem Alzheimers disease brain with 6-fold higher affinity. Chrysamine G is a carboxylic acid analogue of Congo red, binds to senile plaques , also used to stain cerebrovascular amyloid in tissue sections Congo Red *UltraPure Grade* For early diagnosis of amyloid deposition. Monitored by a yellow-green birefringence under crossed polarization. Also, when observed under UV light, it emits fluorescence in amyloid sections. Half Chrysamine G Has lower affinity for Ab than CG. Thioflavin T *UltraPure Grade* Classic amyloid stain for senile plaques containing bA4 peptide in Alzheimers disease brain. Binds to sheets, not to monomers/dimmers, when excited at 450 nm, it emits at 482 nm. 10 mg
88301
-Amyloid (1-40) Tracers -Amyloid (1-40), N-Terminal Labeled (TagDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV)
88302
100 mg
Cat. No. 24235
Product -Amyloid (1-40), N-Terminal Unlabeled Also available in Biotin and fluor labeled form
Qty 0.5 mg
88303
10 mg
83016
1g
DHL Fluorescent beta-Amyloid (1-42) Sampler Kit

Fluorophore labeled beta-amyloid peptides have been used in investigating beta-amyloids aggregation, generation and clearance, microglial activation and phagocytosis. DHL fluorescent b-amyloid (1-42) sampler kit provides seven fluorescent beta-amyloid (142) peptides and two unlabeled peptide as control. The sampler kit provides ample choices for researchers interested in beta-amyloid related experiments. Cat. No. 71022 Product DHL Fluorescent beta-Amyloid (1-42) Sampler Kit Qty 1 kit
88305
10 mg
88306
1g
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93
Substrates, Assay kits and Reagents for Caspases

EnzoLyte AMC Caspase Substrate Sampler Kit *Fluorimetric*
EnzoLyte Caspase Substrate Sample Kit contains a series of AMC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. It provides the best solution for identifying a suitable substrate for designing AMC-based caspase assays. All the components in the kit are also available in bulk package. Cat. No. 71121 Product EnzoLyte AMC Caspase Substrate Sampler Kit *Fluorimetric* Qty 8x100 assays
APOPTOSIS
Rh110-based Caspase Assay kits:

Rh110-based substrates, which yield green fluorescence upon proteolytic cleavage. The kit can be used to continuously measure the activities of caspase-3 in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. The longer wavelength spectrum and higher extinction coefficient of the green-fluorescent Rh110 product provide greater sensitivity and less interference from cell components.
EnzoLyte Rh110 Caspase 3 Assay Kit *Most Sensitive*

EnzoLyte Rh110 Caspase-3 Assay Kit contains (Ac-DEVD)2-Rh110 as a fluorogenic indicator for assaying caspase-3 activities. Cat. No. 71141 Product EnzoLyte Rh110 Caspase 3 Assay Kit *Most Sensitive* Qty 500 assays
Contents: 10 AMC caspase substrates (fluorogenic indicator, Ex/Em=354/422nm upon cleavage), Assay buffer, AMC (fluorescence reference standard for calibration)
AnaRed based Caspase Assay kits:

AnaRed-based substrates, which yield near IR fluorescence upon proteolytic cleavage, are particularly useful for cell-based assays. The kit can be used to continuously measure the activities of caspase-3 in cell culture directly, cell extracts, and purified enzyme using a fluorescence microplate reader or fluorometer.
Contents: (Z-DEVD)2-Rh110 substrate (fluorogenic indicator, Ex/Em=496/520nm upon cleavage), Cell lysis buffer, Assay buffer, Ac-DEVD-CHO (inhibitor), Rh110 (fluorescence reference standard for calibration)
EnzoLyteAnaRed Caspase 3 Assay Kit *Fluorimetric*

EnzoLyte AnaRed Caspase-3 Assay Kit contains Ac-DEVD-AnaRed as a fluorogenic indicator for assaying caspase-3 activities and screening caspase-3 inhibitors. Cat. No. 71122 Product EnzoLyte AnaRed Caspase 3 Assay Kit *Fluorimetric* Qty 500 assays
EnzoLyte Rh110 Caspase 7 Assay Kit *Most Sensitive*

The kit can be used to continuously measure the activities of caspase-7 in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. Cat. No. 71142 Product EnzoLyte Rh110 Caspase 7 Assay Kit *Most Sensitive* Qty 500 assays
Contents: Ac-DEVD-AnaRed substrate (fluorogenic indicator, Ex/Em=595/635nm upon cleavage), AnaRed dye (fluorescence reference standard for calibration), Assay buffer, Ac-DEVD-CHO (inhibitor), Cell lysis buffer
Contents: (Z-DEVD)2-Rh110 substrate (Ex/Em=496/520 nm upon cleavage), Cell lysis buffer, Assay buffer, Ac-DEVD-CHO (inhibitor), Rh110 (fluorescence reference standard for calibration), A detailed protocol
EnzoLyte AnaRed Caspase 7 Assay Kit *Fluorimetric*

The EnzoLyte AnaRed Caspase-7 Assay Kit contains Ac-DEVD-AnaRed as fluorogenic indicator for assaying Caspase-7 activities and screening caspase-7 inhibitors. Cat. No. 71123 Product EnzoLyte AnaRed Caspase 7 Assay Kit *Fluorimetric* Qty 500 assays
EnzoLyte Rh110 Caspase 3 Screening Kit *Most Sensitive*

This EnzoLyte Rh110 Caspase 3 Screening Kit contains (Z-DEVD)2-Rh110 as a fluorogenic indicator for screening caspase-3 inhibitors and inducers. The kit is used to continuously measure the activities of caspase-3 using a fluorescence microplate reader. Cat. No. 71143 Product EnzoLyte Rh110 Caspase 3 Screening Kit *Most Sensitive* Qty 500 assays
Contents: Ac-DEVD-AnaRed substrate (fluorogenic indicator, Ex/Em=595/635nm upon cleavage), AnaRed dye (fluorescence reference standard for calibration), Assay buffer, Ac-DEVD-CHO (inhibitor), Cell lysis buffer, A detailed protocol
Contents: (Z-DEVD)2-Rh110 substrate (Ex/Em=496/520 nm upon cleavage), Assay buffer, Ac-DEVD-CHO (inhibitor), Rh110 (fluorescence reference standard for calibration), A detailed protocol
Caspase Substrates, Chromogenic (pNA)

pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. pNA has maximum absorption around 408 nm. Cat. No. 25250-5 25259-5 Product Ac-YETD-pNA Caspase-1 (ICE) substrate Ac-YVAD-pNA Caspase-1 substrate with Km = 23 mM and kcat = 1.5 s-1 Ac-VDQQD-pNA Caspase-2 substrate Qty 5 mg 5 mg
Caspase substrates, with AnaRed

Cat. No. 60316-1 60318-1 Product D-AnaRed Caspase-2 substrate Z-DEVD-AnaRed Caspase-3 substrate and caspase-8 substrate Ac-IETD-AnaRed Caspase-8 substrate Qty 1 mg 1 mg
60314-1
1 mg
25251-5
5 mg
94
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F : 040-27016168
Substrates for Caspases

Caspase Substrates, Chromogenic (pNA)
Cat. No. 25252-5 Product Ac-VDVAD-pNA Caspase-2 and kcat = 4.5 s-1; Caspase-3 substrate Ac-VQVD-pNA Caspase-3 substrate Z-DEVD-pNA Caspase-3 substrate Ac-DMQD-pNA Caspase-3 substrate Ac-DQMD-pNA Caspase-3 (apopain) substrate Ac-LEVD-pNA Caspase-4 substrate Ac-IETD-pNA Caspase-8 substrate with Km = 66 mM; Substrate for granzyme B Ac-LEHD-pNA Caspase-9 substrate Ac-VEID-pNA Caspase-1, Caspase-3, Caspase-6, Caspase-7 Ac-DEVD-pNA Caspase-1, Caspase-3, Caspase-4, Caspase-6, Caspase-7, Caspase-8 Qty 5 mg
APOPTOSIS
Caspase Substrates, Fluorogenic (AFC)

AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370390 nm. Cat. No. 25271-5 25285-5 25264-5 60325-5 25273-5 Product Ac-YVAD-AFC Caspase-1 substrate Ac-WEHD-AFC Caspase-1 substrate Ac-VDVAD-AFC Caspase-2 substrate Ac-DMQD-AFC Caspase-3 substrate Ac-DEVD-AFC Caspase-3 and Caspase-7 substrate Z-DEVD-AFC Caspase-3 and caspase-7 substrates Ac-LEVD-AFC Caspase-4 substrate Ac-VEID-AFC Caspase-6 substrate Ac-IETD-AFC Caspase-8 substrate Z-IETD-AFC Caspase-8 substrate Ac-LEHD-AFC Caspase-9 substrate Qty 5 mg 5 mg 5 mg 5 mg 5 mg
25253-5 25282-5 25260-5 25265-5 25249-5 25258-5
5 mg 5 mg 5 mg 5 mg 5 mg 5 mg
25278-5 25254-5
5 mg 5 mg
21685-5 28267 25272-5
5 mg 5 mg 5 mg 5 mg 5 mg 5 mg
25263-5
5 mg 25270-5 60324-5
Caspase substrates, with Rh110 peptides

Rh110 (rhodamine 110)-derived caspase substrates are probably the most sensitive indicators widely used for the fluorimetric detection of various caspase activities. Cleavage of Rh110 peptides by caspases generates strongly fluorescent Rh110 that is monitored fluorimetrically at 510-530 nm with excitation of 488 nm, the most common excitation light source used in fluorescence instruments. Cat. No. 60310-1 60309-1 60313-1 60304-5 Product (Ac-YVAD)2-Rh110 Caspase-1 substrate (Ac-WEHD) 2-Rh110 Caspase-1 substrate D2-Rh110 Caspase-2 substrate (Z-DEVD) 2-Rh110 Caspase-3 substrate and caspase-7 substrate (Ac-DMQD) 2-Rh110 Caspase-3 substrate (Ac-LEVD) 2-Rh110 Caspase-4 substrate (Ac-VEID) 2-Rh110 Caspase-6 substrate (Z-IETD) 2-Rh110 Caspase-8 substrate (Ac-LEHD) 2-Rh110 Caspase-9 substrate Qty 1 mg 1 mg 1 mg 5 mg
25276-5
Caspase Substrates, Fluorogenic (AMC)

Cat. No. 28267-5 25275-5 25267-5 25261-5 25255-5 25266-5 25262-5 60303-5 28265 25256-5 25256-5 25257-5 25286-5 Product Ac-YEVD-AMC Caspase-1 (ICE) substrate Ac-WEHD-AMC Caspase-1 substrate with Km/Kcat = 3.3106 M-1s-1 Ac-YEVD-AMC Caspase-1 (ICE) substrate Ac-YVAD-AMC Caspase-1 and Caspase-4 substrate Ac-VDVAD-AMC Caspase-2 substrate Ac-DMQD-AMC Caspase-3 substrate Ac-DEVD-AMC Caspase-3 and Caspase-7 substrate Z-DEVD-AMC Caspase-3 and caspase-7 substrates Ac-LEVD-AMC Caspase-4 substrate Ac-VEID-AMC Caspase-6 substrate Ac-IETD-AMC Caspase-8 substrate Ac-IETD-AMC Caspase-8 substrate Ac-LEHD-AMC Caspase-9 substrate Qty 5 mg 5 mg 5 mg 5 mg 5 mg 5 mg 5 mg 5 mg 5 mg 5 mg 5 mg 5 mg 5 mg
60305-1 60307-1 60308-1 60312-1 60306-1
1 mg 1 mg 1 mg 5 mg 1 mg
Caspase Antibodies
Please inquire
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F : 040-27016168
95
GTP Family- Ras

Small GTPases (Ras Superfamily) - Ras Family
GTPases of the Ras superfamily represent a group of more than fifty proteins that function as molecular switches controlling a variety of signaling and transport pathways essential for numerous cellular functions. They were grouped, according to sequence homologies, into five principal branches: Ras family, Rab family, Ran family, Rho/Rac/Cdc42 family and Sar1/Arf family. Mutations in Ras proteins that block them in a constitutively active state are found in 30 to 60% of human malignancies. Ras-proteins have a molecular mass of 21 kDa. Suitable as a substrate for farnesyltransferase. H-Ras is one of the classic human Ras proteins (H-, N-, K-Ras4A, and KRas4B. H-Ras C comprises the first 166 residues of Ras that are sufficient for binding guanine nucleotides and hydrolysis of GTP, but lacks the C-terminal CaaX motif necessary for membrane localization of Ras. H-Ras C (aa 1 - 171) lacks the C-terminus with the CaaX recognition sequence necessary for anchoring Ras into the plasma membrane. The mutation S17N results in a 40-fold increase in the affinity for GTP without affecting its affinity for GDP. The mutation Q61L results in a decreased GTPase activity as well as increased GDP/GTP exchange. This mutant constitutively activates the Ras-signaling pathway. The mutation G12V leads to elimination of the intrinsic GTPase activity. H-Ras (G12V) is effective in activation of PI3K and PKB, whereas N-Ras and K-Ras are more potent towards MAP kinase. PR-358 K-Ras, one of three classic human Ras genes, is spliced to two different isoforms, K-Ras4A and K-Ras4B. K-Ras4B is the predominant product of the alternative splicing pathway and the Ras protein encoded by the commonly mutated Ras gene in human cancer. PR-204 Stimulation of Ba/F3-Fms cells with either IL-3 or CSF-1 resulted in efficient activation of both K-Ras 4B and M-Ras. Stimulation of fibroblasts with EGF also led to more efficient activation of K-Ras 4B than of H-Ras or NRas, and the activation of M-Ras was very strong. PR-396 An S27N mutant of M-Ras, like the analogous H-Ras S17N mutant, was a dominant inhibitor of activation of the c-fos promoter by constitutively active Src Y527F, suggesting that M-Ras and p21 Ras shared guanine nucleotide exchange factors and are likely to be activated in parallel. MRas (Q71L) is a constitutive active mutant of M-Ras. N-Ras C comprises the first 167 residues of Ras that are sufficient for binding guanine nucleotides and hydrolysis of GTP, but lacks the C-terminal CaaX motif necessary for membrane localization of Ras. PR-359 TC21 is a Ras-like GTPase with high oncogenic potential that is found mutated in some human tumors and overexpressed in breast cancer cell lines. TC21 binds physically to c-Raf-1 in a GTP-dependent manner. TC21 (Q72L) is a constitutive active mutant of TC21. Rap1A, in contrast to Ras, is found largely in mid-Golgi, endocytic vesicles, and lysosomal vesicles, indicating different cellular functions of the proteins. In developmental systems, the functions of Rap proteins seem to be related primarily to morphological and differentiative events, rather than to those that govern proliferation and cell-fate specification, phenomena that often require signaling via conventional Ras proteins. The G12V mutant is a constitutively active mutant of Rap1A. Rap1B is known to antagonize the mitotic and transforming activity of Ras. Rap1B can be activated by cAMP known to either stimulate or inhibit cell proliferation. The deletion of 17 C-terminal amino acids in Rap1B C leads to the loss of the phosphorylation site Ser179 and the geranyl-geranylation site. PR-395 Cat. No. PR-107 Product
SIGNAL TRANSDUCTION
GTP-Ras
Qty 50 g
H-Ras (Harvey rat sarcoma viral oncogene homolog) Human H-RasGST (Harvey rat sarcoma viral oncogene homolog) Human H-Ras C (Harvey rat sarcoma viral oncogene homolog, residues 1-166) Human H-Ras C (S17N) (Harvey rat sarcoma viral oncogene homolog, residues 1-166) Human H-Ras (Q61L) (Harvey rat sarcoma viral oncogene homolog) Human H-Ras (G12V) (Harvey rat sarcoma viral oncogene homolog) Human H-Ras (G12V)GST (Harvey rat sarcoma viral oncogene homolog) Human K-Ras 4B (Kirsten rat sarcoma viral oncogene homolog, isoform 4B) Human M-Ras/R-Ras3His (Q71L) (muscle and microspikes Ras, related ras viral oncogene homolog 3) Human N-Ras C (Neuroblastoma rat sarcoma viral oncogene homolog, residues 1-167) Human TC21/R-Ras2GST (Teratocarcinoma oncogene, related ras viral oncogene homolog 2) Human TC21/R-Ras2His (Q72L) (Teratocarcinoma oncogene, related ras viral oncogene homolog 2, constitutive active mutant) Human Rap1AHis (Ras-proximate 1A) Human Rap1AHis (G12V) (Ras-proximate 1A) Human Rap1B C (Ras-proximate 1B, deletion of 17 C-terminal residues) Human
PR-364
50 g
PR-201
50 g
PR-202
50 g
PR-203
50 g
PR-206
50 g
50 g
50 g
50 g
PR-205
50 g
50 g
50 g
PR-124
50 g
PR-125
50 g
PR-301
50 g
96
! T : 040-27017477 / 65916167
F : 040-27016168
GTP Family- Rab

Small GTPases (Ras Superfamily) - Rab Family
Rab proteins are small GTP-binding proteins that form the largest family within the Ras superfamily. Rab proteins regulate vesicular trafficking pathways, behaving as membrane-associated molecular switches. Rab1A is a small GTPase that belongs to the Ras superfamily. Rab proteins play an important role in various aspects of membrane traffic, including cargo selection, vesicle budding, vesicle motility, tethering, docking, and fusion. For example, Rab1A, Rab1B and Rab2 are located inthe ER to Golgi intermediate compartment and cis Golgi cisternae and are required for ER to Golgi and intra Golgi transport. The small GTPase Rab2 is a resident of pre-Golgi intermediates and is required for protein transport from the endoplasmic reticulum to the Golgi complex. Rab3A, a member of the Rab small G protein family, is involved in the process of Ca 2+-dependent neurotransmitter release. Rab3A activity is regulated by a GDP/GTP exchange protein (Rab3 GEP), a Rab GDP dissociation inhibitor (Rab GDI), and a GTPaseactivating protein (Rab3 GAP). The Rab3A recycling is coupled with synaptic vesicle trafficking. The monomeric GTPase Rab4 is associated with early endosomes and regulates recycling vesicle formation. Together with Rab5 it act to control influx and efflux out of early endosomes. Rab6A is a small GTPase that belongs to the Ras superfamily. It mediates intra-Golgi vesicular trafficking and is geranylgeranylated on both Cterminal cysteines. Rab7 is a 23.5 kDa small GTPase localized in late endosomes. Rab7 regulates late endocytic membrane traffic between endosomes and lysosomes. The protein contains a C-terminal CXC motif for post-translational geranylgeranylation. The small GTPase Rab17 is restricted to epithelial cells and its expression is induced during cell polarization. In addition to the network of ubiquitous Rab GTPases, a set of epithelia-specific Rab proteins, including Rab17, Rab18, Rab20, and Rab25, facilitates endocytic and transcytotic transport to the apical and basolateral plasma membranes. Rab24 exists predominantly in the GTP state when expressed in cultured cells. The low GTPase activity is related to the presence of serine instead of glutamine at the position cognate to Ras Gln-61. Rab24 was found in the endoplasmic reticulum/cis-Golgi region and on late endosomal structures. The localization of Rab24 may indicate its involvement in autophagyrelated processes. The monomeric GTPase Rab27 subfamily regulates the exocytosis of these cell-specific store organelles. Rab27 acts through organelle-specific effector proteins, such as granuphilin in pancreatic beta cells and melanophilin in melanocytes. The Rab27 and effector complex then interacts with proteins that are essential for membrane transport and fusion. Rab GDP Dissociation Inhibitor (RabGDI) forms complexes with cytosolic prenylated GDP-bound Rab proteins and delivers them to the target membrane. It also retreaves GDP-bound Rab proteins from the membrane releases. PR-116 Cat. No. PR-113 Product
SIGNAL TRANSDUCTION
GTP- Rab
Qty 50 g
Rab1AGST-His (Ras-associated, small GTP-binding protein) Mouse Rab1BGST-His (Ras-associated, small GTP-binding protein) Rat Rab2 (Ras-associated, small GTP-binding protein) Human Rab2GST-His (Ras-associated, small GTPbinding protein) Human Rab3AGST-His (Ras-associated, small GTP-binding protein) Mouse Rab4A (Ras-associated, small GTP-binding protein) Human Rab4AGST-His (Ras-associated, small GTP-binding protein) Human Rab6A (Member of Ras oncogene family) Human Rab7 (Ras-associated, small GTP-binding protein) Dog Rab7GST-His (Ras-associated, small GTP-binding protein) Dog Rab17 (Ras-associated, small GTPbinding protein) Mouse Rab17GST-His (Ras-associated, small GTP-binding protein) Mouse Rab24 (Ras-associated, small GTP-binding protein) Mouse Rab24GST-His (Ras-associated, small GTP-binding protein) Mouse Rab27A (Ras-associated, small GTP-binding protein) Rat Rab27AGST-His (Ras-associated, small GTP-binding protein) Rat RabGDI (Rab GDP Dissociation Inhibitor, member of Ras oncogene family) Bovine, Sf9 insect cells
PR-114
50 g
PR-111
50 g
PR-112
50 g
PR-115
50 g
PR-117
50 g
50 g
PR-109
50 g
PR-108
50 g
PR-362
50 g
PR-119
50 g
PR-118
50 g
PR-121
50 g
PR-120
50 g
PR-123
50 g
PR-122
50 g
PR-110
50 g
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97
GTP Family- Ran, Heterotrimeric G-protein

Small GTPases - Ran Family
Ran (Ras-related nuclear protein) is involved in diverse cellular processes like nucleo-cytosplasmic transport of proteins into the nucleus and RNA into the cytoplasm, mitotic spindle assembly, and post-mitotic nuclear assembly. The Ran-regulated nucleo-cytoplasmic transport through the nuclear core complex occurs in conjunction with importins and exportins. The mutation T24N results in no or little binding to GTP and is known to act as a negative inhibitor of nuclear import. Ran Q69L is defective in GTP hydrolysis. Useful as a negative control for Ran binding and import studies. The mutation Q69L abolishes GTP hydrolysis. The mutation G19V results in a gain-of-function mutant that is not, however, sensitive to the exchange factor RCC1. The mutation E70A results in inhibition of the guanine exchange reaction mediated by RCC1, the guanine nucleotide exchange factor (GEF) for Ran. Cat. No. PR-211 PR-212 PR-213 PR-214 PR-215 Product Ran (Ras-related nuclear protein) Human Ran (T24N) (Ras-related nuclear protein) Human Ran (Q69L) (Ras-related nuclear protein) Human Ran (G19V/Q69L) (Ras-related nuclear protein) Human Ran (E70A) (Ras-related nuclear protein) Human Qty 50 g 50 g 50 g 50 g 50 g PR-363 Cat. No. PR-303 Product
SIGNAL TRANSDUCTION
GTP-Ran, Heterotrimer
Qty 50 g
Rac1 C (Ras-related C3 botulinum toxin substrate 1, residues 1-187) Human RhoA (Ras-like GTP-binding protein) Human RhoA GST (Ras-like GTP-binding protein, GST-tagged) Human
PR-106
50 g
50 g
Heterotrimeric G-Proteins
Heterotrimeric G-Proteins are GTPases composed of -, -, and subunits. These GTPases are classically associated with plasma membrane receptors containing seven transmembrane domains (G protein-coupled receptors [GPCRs]). Receptor activation activates the G-Protein by inducing the exchange of GTP for GDP at a binding site on G . G and/or G, then goes on to interact with effector proteins. In mammals, about 20 G subunits (39 to 46 kD), about 5 distinct G (ca. 35 kD) and at least 12 G subunits (from 7 to 10 kD) have been identified. Gs L is the long splice variant of the -subunit of stimulatory heterotrimeric Gs-proteins. It differs from the short splice variant (Gs S) by 15-amino acid insert between the Ras-like domain and the ahelical domain. Gs L activates adenylate cyclase (AC) and possesses a lower GDP-affinity than GsS (cat.# PR-505). These differences in GDPaffinity have important consequences for receptor/G-protein coupling and AC activation. The mutant Gs L-Asn295 is functionally inactive in terms of nucleotide binding. GTP-binding possesses a highly conserved aspartate residue in the NKX D motif that is critical for high-affinity interaction with GTP. In almost all GTP-binding proteins so far, the D/N-mutation switches basespecifity from guanine to xanthine. The exchange of Gln227 to Leu227 inhibits the intrinsic GTPase activity, resulting in a constitutively active G , and increases GDP-affinity of G . Whereas the exchange of Asp295 to Asn295 leads to inactive mutants of G-subunits, an additional Q/L-mutation in the catalytic site (Gln227 Leu227) rescues protein function and induces xanthine nucleotidespecifity. In contrast to all other G -subunits studied so far, the D/N-mutation in Gs S does not influence nucleotide binding activity. The exchange of Gln212 to Leu212 inhibits the intrinsic GTPase activity, resulting in a constitutively activated G , and increases GDP-affinity of G . Gustducin is a transducin-like heterotrimeric guanine nucleotide-binding protein (G-protein) expressed in taste receptor cells (TRCs) of the tongue, stomach and intestinal brush cells. Gustducin was demonstrated to be myristolated and was also palmitoylated in insect cells. Gustducin mediates two responses, i.e . a decrease in cyclic nucleotide monophosphates via activation of phosphodiesterase 1A by gustducin- and activation of phospholipase C- by released G subunits. Gustducin has close structural similarity with the visual G-protein, transducin- . Gustducin- reconstituted with the G-protein subunits G12 was prepared from Sf9 cells coinfected with gustducin- and G 12 -encoding baculoviruses. Cat. No. PR-501 Product G s L (stimulatory heterotrimeric G-protein, long splice variant of the -subunit) Rat G s L-Asn 295 (stimulatory heterotrimeric G-protein, long splice variant of the -subunit) Rat Qty 1 ml
Small GTPases - Rho/Rac/Cdc42 Family

Rho family GTPases Rac1 and Cdc42 (cell division cycle 42) belong to the Ras superfamily of small GTP-binding proteins. The human homolog of yeast Cdc42 is essential for cell polarity and regulates cytoskeletal rearrangements in responses to growth factor stimulation. The C-terminal deletion of 13 amino acids of Cdc42 DC includes the polybasic domain consisting of six contiguous basic amino acids. The polybasic domain of Cdc42 is required for homodimer formation. The Gly12 to Val mutation of Cdc42 DC leads to a constitutive active protein. Rac1 is involved in regulation of cell growth to cytoskeletal organisation required for cell motility and cell adhesion.The C-terminal deletion of five amino acids of Rac1DC (aa 1 - 187) includes arginine 188, which is part of the polybasic domain consisting of six contiguous basic amino acids. Deletion or mutation of the polybasic residues decrease the intrinsic GTPase activity and result in a loss of the self-stimulatory GAP activity. RhoA is a small GTPase of the Ras superfamily. Regulates stress fiber formation in response to growth factor stimulation. Cat. No. PR-302 Product Cdc42 CGST (Cell Division Cycle Protein 42, C-terminal deletion of 13 residues) Human Cdc42 C G12VGST (Cell Division Cycle Protein 42, G12V mutant, C-terminal deletion of 13 residues) Human Qty 50 g
PR-300
50 g
PR-502
1 ml
98
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GTP Family- GTPases, GPCRs

Heterotrimeric G-Proteins
Cat. No. PR-504 Product G s L-Leu 227 (stimulatory heterotrimeric G-protein, long splice variant of the -subunit) Rat G s L -Leu 227 -Asn 295 (stimulatory heterotrimeric G-protein, long splice variant of the -subunit) Rat G s S (stimulatory heterotrimeric G-protein, short splice variant of the -subunit) Rat G s S -Asn 280 (stimulatory heterotrimeric G-protein, short splice variant of the -subunit) Rat G s S-Leu 212 (stimulatory heterotrimeric G-protein, short splice variant of the -subunit) Rat G s S-Leu 212 -Asn 280 (stimulatory heterotrimeric G-protein, short splice variant of the -subunit) Rat Gustducin + 12 (Gustducin + G-protein 12-subunits) Rat Qty 1 ml
SIGNAL TRANSDUCTION
GTP Heterotrimer, GAP, GTPase
GAPs (GTPase Activation Proteins)

GTPase activating proteins (GAPs) accelerate the GTP hydrolysis reaction by up to 5 orders of magnitude, thus switching off the activated GTPase and terminating the signaling activity. The malignant transformation of cells is often linked to oncogenic mutations of Ras that escape regulation by GAPs. The NF1 (Neurofibromin) gene product acts as a GTPase activating factor (GAP) on Ras. Inactivating mutations in NF1 lead to neurofibromatosis type 1. NF1- 333 contains residues 1198-1530 of human NF1 comprising the functional GAP-related domain that is able to stimulate GTP-hydrolysis on wild type Ras. RapGAP, the GTPase activating protein of Rap, catalyzes the transition of Rap from GTP-bound to GDP-bound state by hydrolysis of GTP. Rap proteins are GTPases of the Ras superfamily of G proteins opposing the Ras activation pathway. RapGap-341 is a catalytic active fragment comprising 341 amino acids (residues 75 - 415) of wild type Rap1GAP. RapGAP-341 (R91A) retained more than 50% activity in comparison to RapGAP-341. Rna1p is the GTPase-activating factor (GAP) of yeast Gsp1p, an ortholog of mammalian Ran. Rna1p is located in the cytoplasm throughout the cell cycle and plays a direct role in protein import into the nucleus in S. cerevisiae and S. pombe. Cat. No. 1 ml PR-223 Product NF1-333 (RasGAP) (Neurofibromin, GTPase Activating Protein of Ras) Human RapGAP-341 (R91A) (GTPase Activating Protein of Rap) Human Rna1p (RanGAP) (GTPase Activating Factor of yeast Gsp1p, an ortholog of mammalian Ran) S. pombe Qty 50 g
PR-503
1 ml
PR-505
1 ml
PR-507
1 ml
PR-508
1 ml
PR-506
1 ml
PR-601
PR-222
50 g
GEFs (Guanine Nucleotide Exchange Factors)

EPAC (exchange protein directly activated by cAMP) is a Rap-specific guanine-nucleotide exchange factor (GEF). EPAC is activated by the binding of cAMP to a cyclic nucleotide monophosphate-binding domain. Nterminal deletion of the DEP domain (domain that occur in Dishevelled, Egl-10, and Pleckstrin) of EPAC DEP does not affect regulation of EPACactivity but affects membrane localization. RCC1 (Regulator of Chromosome Condensation, a -propeller chromatinbound protein) catalyzes guanine nucleotide exchange of the Ras-related nuclear protein Ran. RCC1 is the guanine nucleotide exchange factor (GEF) for Ran that induces exchange between the bound GDP and cellular GTP. Genetic studies have suggested that RCC1 may be involved in sensing the replication state of DNA and controlling the activation of Cdc2/ cyclin B protein kinase through Ran. Ras-binding domain (RBD) of the human RalGDS, comprises amino acids 1 - 97 of wild type. RalGDS (Ral-guanine nucleotide dissociation stimulator) is the guanine nucleotide exchange factor (GEF) for the Ras-related protein Ral. The GEF-activity of RalGDS is stimulated by binding of Ras. PR-221
50 g
GTPase Cycle Effectors

Raf is a member of the serine/threonine proteine kinase family involved in regulation of cell growth and differentiation and is the most important effector of Ras. RafRBD (Ras binding domain, amino acids 50-132) mediates interaction with membrane-anchored Ras necessary for activation of the kinase activity of Raf. Ras-binding domain (RBD) of the human RalGDS, comprises amino acids 1 - 97 of wild type. RalGDS (Ral-guanine nucleotide dissociation stimulator) is the guanine nucleotide exchange factor (GEF) for the Ras-related protein Ral. The GEF-activity of RalGDS is stimulated by binding of Ras. Rhotekin is an effector of the Rho-GTPases that was previously identified in mice. Cat. No. PR-305 Product RafRBD (c-Raf) (Ras Binding Domain of Raf, c-Raf 1 Kinase) Human RafRBDGST (c-Raf) (Ras Binding Domain of Raf, c-Raf 1 Kinase) Human RalGDS-RBDGST (Ras Binding Domain of the Ral Guanine Nucleotide Dissociation Stimulator) Human RhotekinRBDGST (Rhotekin Ras Binding Domain, from the Japanese Human Qty 50 g
Cat. No. PR-304
Product EPAC-1 DEP (Exchange Protein directly Activated by cAMP, lacks the Dishevelled, Egl-10 and Pleckstrin Domain) Human RCC1 (RanGEF) (Regulator of Chromosome Condensation, Ran Guanine Exchange Factor) Human RalGDS-RBD (Ras Binding Domain of the Ral Guanine Nucleotide Dissociation Stimulator) Human
GST
Qty 50 g
PR-366 50 g PR-365 50 g
50 g
PR-306
50 g
PR-365
PR-367
50 g
! T : 040-27017477 / 65916167
F : 040-27016168
99
GTP Family- Ran, Heterotrimeric G-protein

GTPase Cycle Effectors
The Wiskott-Aldrich syndrome protein (WASP) is the founding member of a family of proteins that have emerged as crucial effectors of Rho GTPases and activators of the cytosceletal organizing complex Arp2/3. WASP has been shown to be intimately involved in many pathways that influence the function of the immune system. WASP-121 comprises the amino acids 201-321 of the native WASP sequence. This domain is essential for the binding of Cdc42 to WASP. EPAC (exchange protein directly activated by cAMP) is a Rap-specific guanine-nucleotide exchange factor (GEF). EPAC is activated by the binding of cAMP to a cyclic nucleotide monophosphate-binding domain. Nterminal deletion of the DEP domain (domain that occur in Dishevelled, Egl-10, and Pleckstrin) of EPAC DEP does not affect regulation of EPACactivity but affects membrane localization. The amino terminus of Af6 was identified as the Ras-binding site. Additionally to the Ras-binding Af6 also binds Rap. ZO-1, a protein involved in the formation of tight junctions, also binds to Af6 close to the amino terminus thereby competing with Ras binding. These data suggest a participation of Af6 in the regulation of cell-cell contacts via a Rasmodulated interaction with ZO-1. RIN2, only expressed in neurons, preferentially interacts with the GTP form of Rab5 rather than the GDP form, although it enhances the guanine exchange reaction on Rab5. Additionally RIN2 interacts with several Ras proteins, it binds the GTP-loaded forms of H-Ras, TC21 and M-Ras.
SIGNAL TRANSDUCTION
GTPase, GPCRs
GTPase Modifying Enzymes

REP-2 (Rab escort protein-2) binds to a wide range of RabGGTase with nanomolar affinities and supports the prenylation of RabGTPase mediated by RabGGTase, with an exception of Rab27. Cat. No. PR-101 PR-360 PR-103 Product GGTase-I (Geranyl-Geranyl-Transferase) Rat GGTase-IGST (Geranyl-Geranyl-Transferase) Rat GGTase-II (Rab GGTase) (Geranyl-Geranyl-Transferase, - and -subunit) Rat FTase (Protein farnesyltransferase, - and -subunit) Rat FTaseGST (Protein farnesyltransferase, - and -subunit) Rat REP-1 His (Rab Escort Protein) Rat REP-2 His (Rab Escort Protein) Human Qty 50 g 50 g 50 g
PR-102
50 g
PR-361
50 g
PR-105 PR-104
50 g 50 g
Cat. No. PR-369
Product WASP-121 GST (Wiskott-Aldrich Syndrome Protein) Human EPAC-1 DEP (Exchange Protein directly Activated by cAMP, lacks the Dishevelled, Egl-10 and Pleckstrin Domain) Human AF6-RBDGST (residues 1-141) (GTP-H-Ras binding cytoplasmic protein, Afadin - Ras-binding domain) Human RIN2-RAGST (residues 785-879) (Ras and Rab interactor 2 - Rasassociation domain) Human
Qty 50 g
PR-304
50 g
GPCRs (G-Protein Coupled Receptors) Adrenergic Receptors

The human 1-adrenoreceptor (1AR) is activated by the catecholamines epinephrine and norepinephrine and couples to the G-protein Gs to mediate adenylate cyclase activation. The 1AR exists as several polymorphic forms of which the Gly389 and Arg 389 variants are among the best known. There is a controversy whether or not there are functional differences between the two 1AR polymorphisms. 1ARs are mainly found in the heart, kidney, and fat tissue. These receptors are involved in physiological processes such heart contraction, renin release and lipolysis.. 1-Adrenergic receptor-Gly 389-Gs L is a fusion protein in which the Gs L N-terminus is linked to the 1 -adrenoceptor (1AR) C-terminus via a hexahistidine (His6)-tag. Gs L is the long splice variant of the -subunit of the heterotrimeric G-protein Gs. The 1AR-Gly389-Gs L fusion protein ensures a defined 1:1 stoichiometry of the receptor and the Gs L subunit as well as high coupling efficiency. In contrast to 1AR-Arg389Gs S (cat.# PR-524), 1AR-Gly389-Gs L exhibits hallmarks of high constitutive activity, i.e. high efficacy and potency of partial agonists at activating [ 35S]GTP S binding and high efficiency of agonist-free 1ARGly389-Gs L at activating AC. 1-Adrenergic receptor-Arg389-Gs L is a fusion protein in which the Gs?L N-terminus is linked to the 1-adrenoreceptor ( 1AR) C-terminus via a (His6)-tag. The 1AR exists as several polymorphic forms of which the Gly 389 and Arg 389 variants are among the best known. 2-Adrenergic receptorCAM-Gs L is a fusion protein in which the Gs L N-terminus is linked to the 2AR CAM C-terminus via (His6)-tag. 2AR CAM is a constitutively active mutant of the 2AR. It differs from the wild-type receptor by four discrete amino acid substitutions in the third intracellular loop of the receptor (L266 S266, K267 R267, H269 K269, and L272 A272). 2-Adrenergic receptor-Gs L is a fusion protein in which the Gs L N-terminus is linked to the 2-adrenoceptor (2AR) C-terminus via a (His6)-tag.
PR-398
50 g
PR-397
50 g
GTPase Modifying Enzymes

GGTase-I (Geranylgeranyltransferase-I) catalyzes the transfer of the farnesyl and geranylgeranyl groups from farnesyl and geranylgeranyldiphosphate to proteins containing a C-terminal CaaX motif. Farnesyltransferase (FT) and GGTase-I are closely related, sharing a common a subunit and 30% identity in their subunits. GGTase-II (Geranylgeranyltransferase-II) catalyzes the transfer of geranylgeranyl moiety onto two C-terminal cysteines of Rab proteins. Composed of an and heterodimer (50 and 38 kDa, respectively) and requires Rab escort protein for its catalytic activity. GGTase-II was shown to exhibit higher affinity towards geranylgeranyl pyrophosphate (Kd = 8 nM) than farnesyl pyrophosphate (Kd = 60 nM). FTase catalyzes the transfer of the farnesyl group from farnesyl diphosphate to proteins containing a C-terminal CaaX motif, where C is a conserved cysteine that is the site of farnesyl modification, a is usually an aliphatic amino acid, and X is methionine, serine, glutamine, or alanine. REP-1 (Rab escort protein-1) binds to a wide range of RabGGTase with nanomolar affinities and supports the prenylation of RabGTPase mediated by RabGGTase.
100
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F : 040-27016168
GTP Family- GPCRs

GPCRs (G-Protein Coupled Receptors) Adrenergic Receptors
2-Adrenergic receptor-GsL-Leu227 is a fusion protein in which the GsLLeu 2 2 7 N-terminus is linked to the 2-adrenoceptor ( 2 AR) C-terminus via a (His6)-tag.The exchange of Gln227 to Leu227 (Q/L mutation) in the catalytic site abolishes the intrinsic GTPase activity and increases the GDP-affinity of Gs , resulting in a constitutively active G-protein. 2-Adrenergic receptor-Gs L-Leu 227-Asn 295 is a fusion protein in which the Gs L-Leu227-Asn295 N-terminus is linked to the 2-adrenoceptor (2AR) C-terminus via (His6)-tag. The exchange of Asp 295 to Asn 295 leads to an inactive Gs -mutant. However, an additional Q/L-mutation in the catalytic site (Gln 227 Leu 227) that abolishes GTPase activity and increases GDP-affinity rescues protein function and induces specificity for XTP and XppNHp relative to GTP and GppNHp, respectively. 2 -Adrenergic receptor-Gs L is a fusion protein in which the Gs L N-terminus is linked to the 2AR C-terminus via a (His6)-tag. G 12 are subunits of the heterotrimeric G-protein. Cat. No. PR-521 PR-522 PR-525 PR-524 Product 1-AR-Gly 389 (1-Adrenergic Receptor) Human 1-AR-Gly 389-G s L (1-Adrenergic Receptor GsL fusion protein) Human 1-AR-Arg -G s L (1-Adrenergic Receptor GsL fusion protein) Human
389
SIGNAL TRANSDUCTION
GPCRs
Cat. No. PR-541
Product 2-AR-G i 3 (2-Adrenergic Receptor Gi3 fusion protein) Human 2-AR-Gi3 + 1 2 (2-Adrenergic Receptor Gi3 fusion protein + 1 2-subunits) Human 2-AR-Gq + 1 2 (2-Adrenergic Receptor Gq fusion protein + 12-subunits) Human 2-AR-G i 3 (2-Adrenergic Receptor Gi3 fusion protein) Human 2 -AR-G s S (2-Adrenergic Receptor GsS fusion protein) Human 2-AR-G s S-Asn 280 (2-Adrenergic Receptor GsS fusion protein) Human, Recombinant, Sf9 insect cells 2 -AR-G s S -Leu 212-Asn 280 (2-Adrenergic Receptor GsS fusion protein) Human 2 -AR-G s S-Leu 212 (2-Adrenergic Receptor GsS fusion protein) Human 2-AR + GsS (2-Adrenergic Receptor + GsS) Human 2-AR-G 16 (2-Adrenergic Receptor G16 fusion protein) Human 2-AR-G16 + 1 2 (2-Adrenergic Receptor G16 fusion protein + 12-subunits) Human
Qty 1 ml
PR-551
1 ml
PR-542
1 ml
PR-543
1 ml
PR-544
1 ml
PR-545 Qty 1 ml 1 ml PR-547 1 ml PR-548 1 ml PR-549 1 ml 1 ml PR-550 PR-546
1 ml
1 ml
1 ml
1 -AR-Arg 389 -G s S (1-Adrenergic Receptor GsS fusion protein) Human 1-AR-Gly 389-G s S (1-Adrenergic Receptor GsS fusion protein) Human 2-AR CAM -G sL (Constitutively Active Mutant of 2-Adrenergic Receptor GsL fusion protein) Human 2-AR-G s L (2-Adrenergic Receptor GsL fusion protein) Human 2-AR-G saL-Leu 227 (2-Adrenergic Receptor GsL fusion protein) Human 2-AR-G saL-Leu 227-Asn 295 (2-Adrenergic Receptor GsL fusion protein) Human 2-AR-GsaL + 1 2 (2-Adrenergic Receptor GsL fusion protein + 12subunits) Human 2-AR-TS-G s L (2-Adrenergic Receptor GsL fusion protein with a thrombin cleavage site) Human 2-AR + GsL (2-Adrenergic Receptor + GsL) Human 2-AR-G q (2-Adrenergic Receptor Gq fusion protein) Human 2-AR-G i 2 (2-Adrenergic Receptor Gi2 fusion protein) Human 2-AR-Gi2 + 12 (2-Adrenergic Receptor Gi2 fusion protein + !2-subunits) Human
1 ml 1 ml
PR-523 PR-531
1 ml
PR-532 PR-533 PR-534
1 ml 1 ml 1 ml
GPCRs (G-Protein Coupled Receptors) Histamine Receptors

The histamine H1-receptor (H1R) belongs to the superfamily of seven transmembrane-domain (7TM), G-protein-coupled receptors (GPCRs). The endogenous agonist for the H1R is histamine, which is both neurotransmitter and autacoid. The H1R couples to Gq-proteins to activate phospholipase C. Numerous agonists and antagonists are known. H1R agonists are divided into three classes, i.e. small agonists derived from histamine, histamine derivatives with bulkier aromatic substituents at position 2 of the imidazole ring and histaprofidens. The regulator of G-protein signaling 4 (RGS4) efficiently enhances steadystate GTP hydrolysis stimulated by H1R, thus providing a very sensitive model system for the pharmacological analysis of the H1R directly at the G-protein level. The guinea pig H2R (gpH2R) was prepared from Sf9 cells infected with gpH2R-encoding baculovirus. In this system, gpH2R couples to the Gslike G-proteins of the insect cells to mediate AC activation. hH2R-Gq is a fusion protein in which the Gq N-terminus is linked to the C-terminus of the human H2-receptor (H2R) via a (His6)-tag. hH2R-GsL is a fusion protein in wich the GsL N-terminus is linked to the C-terminus of the human H2-receptor (hH2R) via a (His6)-tag.
PR-535
1 ml
PR-536
1 ml
PR-537 PR-538
1 ml 1 ml
PR-539 PR-540
1 ml 1 ml
hH2R-GsS is a fusion protein in wich the GsS N-terminus is linked to the C-terminus of human H2-receptor (hH2R) via a (His6)-tag. hH2R reconstituted with Gs S was prepared from Sf9 cells coinfected with hH2R- and Gs S-encoding baculoviruses.
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101
GTP Family- GPCRs

GPCRs (G-Protein Coupled Receptors) Histamine Receptors
Cat. No. PR-611 PR-612 Product gpH 1 R (Histamine H1-Receptor) Guinea pig gpH1R + RGS4 (Histamine H1-Receptor + Regulator of G-protein Signaling 4) Guinea pig gpH 2 R (Histamine H2-Receptor) Guinea pig hH 1R (Histamine H1-Receptor) Human hH1R + RGS4 (Histamine H1-Receptor + Regulator of G-protein Signaling 4) Human hH 2R (Histamine H2-Receptor) Human hH 2R-G q (Histamine H2-Receptor Gq fusion protein) Human hH 2R-G s L (Histamine H2-Receptor GsL fusion protein) Human hH 2R-G s S (Histamine H2-Receptor GsS fusion protein) Human rH 2 R (Histamine H2-Receptor) Rat hH2R + GsS (Histamine H2-Receptor + GsS) Human Qty 1 ml 1 ml
SIGNAL TRANSDUCTION
GPCRs
GPCRs (G-Protein Coupled Receptors) Dopamine Receptors

The dopamine D 1-receptor (D 1R) belongs to the superfamily of seven transmembrane-domain (7TM), G-protein-coupled receptors (GPCRs). The D 1R couples to the G-protein Gs to mediate adenylyl cyclase (AC) activation. The endogenous catecholamine dopamine influences many cellular activities including behavior, hormone synthesis, blood pressure control and intracellular ion transport. The human D1R was prepared from Sf9 cells infected with D 1R-encoding baculovirus. In this system the D1R couples to the Gs -like G-proteins of the insect cells to mediate AC activation. Cat. No. PR-581 1 ml Product Dopamine Receptor D 1R Human Qty 1 ml
PR-613 PR-614 PR-615
1 ml 1 ml
PR-616 PR-617
1 ml 1 ml
GPCRs (G-Protein Coupled Receptors) Formyl Peptide Receptors

The formyl peptide receptor (FPR) is expressed in neutrophils, couples to pertussis toxin (PTX)-sensitive Gi-proteins and activates phospholipase C, chemotaxis and cytotoxic cell functions. The FPR, like most GPCRs, is a type IIIb membrane protein with an extracellular N-terminus without a signal sequence. The human FPR exists in various isoforms, FPR-26, FPR-98 and FPR-G6 respectively. These FPR isoforms differ from each other in amino acid positions 101 (localized at the top of the third transmembrane domain), 192 (localized in the center of the second extracellular loop) and 346 (localized at the extreme C-terminus). FPR-26/FPR-98/FPRG6 is reconstituted with the G-protein Gi 21 2 is constitutively active and was prepared from Sf9 cells triple-infected with FPR-, Gi 2-, and G 12-encoding baculoviruses. The human formyl peptide receptor (FPR) is N-glycosylated and activates phagocytes via Gi-proteins. The FPR possesses two potential N-glycosylation sites in the N-terminus (Asn4 and Asn10) and one potential N-glycosylation site in the second extracellular loop (Asn179). FPR-NGII lacks the N-glycosylation sites at Asn4 and Asn10. FPR-NGII reconstituted with the G-protein Gi 212 was prepared from Sf9 cells triple-infected with FPR-, Gi2-, and G12-encoding baculoviruses. Compared to wildtype FPR, FPR-NGII is much less active in terms of high-affinity agonist binding and agonist-stimulated [35S]GTPgS binding. Cat. No. PR-591 Product FPR26 + Gi21 2 (Formyl Peptide Receptor + inhibitory G-protein Gi212) Human FPR98 + Gi21 2 (Formyl Peptide Receptor + inhibitory G-protein Gi212) Human FPRG6 + Gi21 2 (Formyl Peptide Receptor + inhibitory G-protein Gi212) Human, Recombinant, Sf9 insect cells FPR-NGII + Gi21 2 (Formyl Peptide Receptor, non-glycosylated, + inhibitory G-protein Gi212) Human, Recombinant, Sf9 insect cells Qty 1 ml
PR-618
1 ml
PR-619
1 ml
PR-620 PR-621
1 ml 1 ml
GPCRs (G-Protein Coupled Receptors) Leukotriene Receptors

The human leukotriene B4 receptor (BLTR) is a seven transmembranedomain (7TM), G protein-coupled receptor (GPCR). BLTR reconstituted with the G-protein Gi 11 2 was prepared from Sf9 cells tripleinfected with BLTR-, Gi 1-, and G 12-encoding baculoviruses. BLTR reconstituted with the G-protein Gi 21 2 was prepared from Sf9 cells triple-infected with BLTR-, Gi2-, and G12-encoding baculoviruses. BLTR reconstituted with the G-protein Gi 21 2 was prepared from Sf9 cells triple-infected with BLTR-, Gi3-, and G12-encoding baculoviruses. Cat. No. PR-560 Product BLTR + Gia112 (Leukotriene B4 Receptor + inhibitory G-protein Gia112) Human BLTR + Gia212 (Leukotriene B4 Receptor + inhibitory G-protein Gia212) Human BLTR + Gia312 (Leukotriene B4 Receptor + inhibitory G-protein Gia312) Human Qty 1 ml
PR-592
1 ml
PR-593
1 ml
PR-594 1 ml
1 ml
PR-561
PR-562
1 ml
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GTP Family- GPCRs, Importins

GPCRs (G-Protein Coupled Receptors) Complement Component 5a Receptors
The anaphylatoxin C5a-receptor (C5aR, CD88) belongs to the superfamily of seven transmembrane-domain (7TM), G protein-coupled receptors (GPCRs). In monocytes and granulocytes, activation of C5aR causes chemotaxis, degranulation, and superoxide anion production. C5aR is also involved in the formation of adhesion molecules as well as in the production of so-called acute-phase proteins. C5aR reconstituted with the G-protein G i11 2 was prepared from Sf9 cells triple-infected with C5aR-, Gi1-, and G12-encoding baculoviruses. Cat. No. PR-571 Product C5aR + Gi112 (Complement Component 5a Receptor + inhibitory G-protein Gi112) Human, Recombinant, Sf9 insect cells C5aR + Gi212 (Complement Component 5a Receptor + inhibitory G-protein Gi212) Human, Recombinant, Sf9 insect cells C5aR + Gi312 (Complement Component 5a Receptor + inhibitory G-protein Gi312) Human, Recombinant, Sf9 insect cells Qty 1 ml
SIGNAL TRANSDUCTION
GPCRs, Importins
GPCRs (G-Protein Coupled Receptors) Cell Extracts

This membrane preparation was made from uninfected Sf9 insect cells and can be used as a negative control for immunoblotting. Specifically, membranes from uninfected Sf9 cells do not show immunoreactivity with the M1-antibody (reacting with the FLAG epitope) and commonly used antibodies against mammalian Gs , Gi and Gq . Membranes from uninfected Sf9 cells exhibit low but clearly detecable adenylate cyclase activity that can be used as background to assess the effects of exogenously expressed receptors and G-proteins. Cat. No. PR-634 Product Membrane preparation (Sf9 cell extract) uninfected, Sf9 insect cells Qty 1 ml
Importins
1 ml Importins are involved in nuclear import and belong to a seperate class of Ran-GTP binding proteins. Importin itself does not interact with nuclear pore complex (NPC) but functions as an adaptor that binds Importin . Both these importins inhibit mitotic spindle assembly. Importin binds the importin -NLS complex or other adaptors to mediate the uptake of the substrateimportin a/b complex through the nuclear pore complex. Importin and inhibit mitotic spindle assembly. Cat. No. Product Importin His Human Importin His D. melanogaster Qty 50 g
PR-572
PR-573
1 ml
GPCRs (G-Protein Coupled Receptors) Platelet-activating Factor Receptors

Platelet-activating factor (PAF) is a pro-inflammatory lipid mediator possessing a unique 1-O-alkyl-2-acetylsn- glycero-3-phosphocholine backbone. The PAF receptor (PAFR), which belongs to the superfamily of seven transmembrane (7TM), G-proteincoupled receptors (GPCRs), transduces pleiotropic functions including cell motility, smooth muscle contraction, and synthesis and release of mediators and cytokines via multiple heterotrimeric G-proteins. PAFR is reconstituted with the G-protein G i112 was prepared from Sf9 cells triple-infected with PAFR-, Gi1-, and G12-encoding baculoviruses. Cat. No. PR-631 Product PAFR + Gi112 (Platelet Activating Factor Receptor + inhibitory G-protein Gi112) Human, Recombinant, Sf9 insect cells PAFR + Gi212 (Platelet Activating Factor Receptor + inhibitory G-protein Gi212) Human, Recombinant, Sf9 insect cells PAFR + Gia312 (Platelet Activating Factor Receptor + inhibitory G-protein Gia312) Human, Recombinant, Sf9 insect cells Qty 1 ml
PR-231
PR-232
50 g
PR-632
1 ml
PR-633
1 ml
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103
PI3 Kinase Family

Phosphoinositide 3-kinase (PI3K) isoforms native proteins, affinity-tagged variants and associated products
Phosphoinositide 3-kinases (PI3Ks) phosphorylate phosphatidylinositols (PIs) at their 3-OH position generating lipid second messengers and thereby regulate numerous biological processes including cell growth, differentiation, survival, proliferation, migration and metabolism. On the basis of structural similarities and substrate specificity, the PI3K family can be subdivided into three classes termed I, II, and III. Cat. No. PR-344S Product
SIGNAL TRANSDUCTION
P13K
Qty 5 g
PI(3)K, S pack (Phosphoinositide 3-Kinase , p110/p85) Human PI(3)K, L pack (Phosphoinositide 3-Kinase , p110/p85) Human
PR-344L
10 g
PI3K
PI3Ka plays a specific role in apoptosis in human colon cancer cells. Injection of neutralizing antibodies specific to PI3K into adenocarcinoma cells induced apoptosis, a response that was reverted by treating cells with caspase inhibitor. It was also shown that PI3K mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3-phosphorylated phosphoinositides. PI3K is a key regulator of the initiation of keratinocyte differentiation. A decrease in PI3K activity results in a loss of keratinocyte adhesion to the extracellular membrane and the initiation of early phase differentiation. The PI3K catalytic and regulatory subunits are coexpressed in Sf9 insect cells. Cat. No. PR-341S Product PI(3)K, S pack (Phosphoinositide 3-Kinase a, p110/p85) Bovine, Recombinant, Sf9 insect cells PI(3)K, L pack (Phosphoinositide 3-Kinase , p110/p85) Bovine, Recombinant, St9 insect cells PI(3)KGST, S pack (Phosphoinositide 3-kinase p110/p85) Bovine, Recombinant, Sf9 insect cells PI(3)KGST, L pack (Phosphoinositide 3-kinase p110/p85) Bovine, Recombinant, Sf9 insect cells PI(3)KR916PGST, S pack (Phosphoinositide 3-kinase p110, p85) Bovine, Recombinant, Sf9 insect cells PI(3)KR916PGST, L pack (Phosphoinositide 3-kinase p110, p85) Bovine, Recombinant, Sf9 insect cells p85 (Phosphoinositide 3-Kinase , regulatory subunit) Bovine, Recombinant, Qty 5 g
PI3K
PI3K is highly expressed in cells of hematopoietic origin. It plays an important role in dendritic cell (DC) trafficking and in the activation of specific immunity. PI3K -/- mice showed a reduced ability to respond to chemokines, and had a selective defect in the number of skin Langerhans cells and in lymph node CD8a-DCs. In macrophages, the chemokine RANTES/CCL5 activates the small GTPase Rac1 and its downstream target PAK2. The PI3K D946A protein is a catalytically inactive mutant of PI3K with a D946A mutation in the ATP binding site. This recombinant catalytically inactive protein can be used as a negative control in any kind of PI3Kg kinase activity studies.Recombinant full length PI3KDA mutant carries a N-terminal GST-Tag and was purified by affinity chromatography. Distinct regions within the p101 regulatory subunit primary stucture are responsible for interaction with PI3K and G . The PI3K binding site is confined to the N-terminus, whereas binding to Gb is mediated by the C-terminus of p101. The PI3K catalytic and regulatory subunits are coexpressed in Sf9 insect cells. The PI3K catalytic subunit carries a N-terminal His6 affinity Tag, whereas the p101 adaptor subunit carries a N-terminal GST-Tag.
PR-341L
10 g
PR-940S
5 g
Cat. No. PR-343S
Product PI(3)KHis, S pack (Phosphoinositide 3-Kinase , p110) Human, Recombinant, Sf9 insect cells PI(3)KHis, L pack (Phosphoinositide 3-Kinase , p110) Human, Recombinant, Sf9 insect cells PI(3)KD946AGST, S pack (Phosphoinositide 3-kinase , p110, catalytically inactive mutant) Human, Recombinant, Sf9 insect cells PI(3)KD946AGST, L pack (Phosphoinositide 3-kinase , p110, catalytically inactive mutant) Human, Recombinant, Sf9 insect cells PI(3)KHis/p101GST, S pack (Phosphoinositide 3-Kinase , p110/p101) Human, Recombinant, Sf9 insect cells PI(3)KHis/p101GST, L pack (Phosphoinositide 3-Kinase , p110/p101) Human, Recombinant, Sf9 insect cells
Qty 5 g
PR-940L
10 g PR-343L 5 g PR-346S 10 g
10 g
PR-941S
5 g
PR-941L
PR-342
20 g
PR-346L
10 g
PI3K
The PI3K isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation of PI3K is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2). It was also shown that PI3K is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr, HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis. PI3K is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line. It was shown by several research groups that the catalytic subunit of PI3K can be activated by G .
PR-347S
5 g
PR-347L
10 g
104
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PI3 Kinase Family

PI3K
Recently it was shown that the inactivation of PI3K in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgEinduced degranulation and cytokine release. In neutrophils a role for PI3K in TNF -induced signalling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the -specific inhibitor IC87114. The PI3K catalytic and regulatory subunits are coexpressed in Sf9 insect cells. The catalytic subunit carries a GST-Tag and the heterodimer was purified by affinity chromatography. Cat. No. PR-345S Product PI(3)KGST, S pack (Phosphoinositide 3-Kinase , p110/p85) Human PI(3)KGST, L pack (Phosphoinositide 3-Kinase , p110/p85) Human Qty 5 g LI-002 10 g LI-009
SIGNAL TRANSDUCTION
PI3K
PI3 Kinase Family Substrates

Cat. No. LI-011 LI-012 LI-001 Product PI3K Lipid Kinase Substrate Mix 1 (PI, PE, PS, PC, and SM) PI3K Lipid Kinase Substrate Mix 2 (PIP2, PE, PS, PC, and SM) PI (L--Phosphatidylinositol) Bovine liver, sodium salt PI [L--Phosphatidylinositol-(1,2dipalmitoyl)-D-myo-inositol] synthetic PI-4-P (L--Phosphatidylinositol-4-phosphate) Porcine brain, diammonium salt PI-4-P [L--Phosphatidylinositol(1,2-dipalmitoyl) -D-myo-inositol 4-monophosphate] synthetic PI-4,5-P 2 (L--Phosphatidylinositol-4,5bisphosphate) Porcine brain, triammonium salt PI-4,5-P 2 [L--Phosphatidylinositol(1,2-dipalmitoyl) -D-myo-inositol 4,5-bisphosphate] synthetic PC (L--Phosphatidylcholine) Chicken egg PE (L--Phosphatidylethanolamine) Chicken egg PS (L--Phosphatidylserine) Porcine brain, sodium salt SM (Sphingomyelin) Porcine brain Qty 1 mg (based on PI) 0.1 mg (based on PIP2) 5 mg
LI-008
100 g
1 mg
PR-345L
100 g
PI3 Kinase Family - Adaptor Proteins

p85 functions as the regulatory subunit of the class IA PI3-kinase isoforms , , and . It contains two SH2 domains that bind to tyrosine-phosphorylated growth factor receptors or substrate adaptor proteins. It also contains a BH (breakpoint cluster region homology) domain that shows GAP activity towards the small GTPases Rab4, Rab5, Cdc42, Rac1 and to a lesser extend towards Rab6 and Rab11. It was shown that PI3K catalytic subunit mediated phosphorylation of the p85 adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3Kdependent signaling events not requiring production of 3-phosphorylated phosphoinositides. PI3K protein kinase activity has been implicated in IRS-1 serine phosphorylation in insulin-treated adipocytes and in STAT3 and IRS-1 phosphorylation upon activation of the type 1 IFN receptor by IFN- . Cat. No. PR-342 Product p85 (Phosphoinositide 3-Kinase , regulatory subunit) Bovine, Recombinant, Leishmania tarentolae Qty 20 g LI-006 LI-003
0.5 mg
LI-010
100 g
LI-004
5 mg
LI-005
5 mg
5 mg
LI-007
5 mg
PI3 Kinase Family Inhibitors

Cat. No. INH-001 Product Wortmannin (PI3-Kinase Inhibitor) Talaromyces wortmannin KY 12420 LY294002 (PI3-Kinase Inhibitor) Quercetin (Protein Kinase Inhibitor) Myricetin (Protein Kinase Inhibitor) Staurosporine (Protein Kinase Inhibitor) Streptomyces staurosporeus Qty 1 mg Cat. No. ABD-026S 1 mg 100 mg 5 mg ABD-027 50 mg ABD-027P ABD-026L
PI3 Kinase Antibodies

Product -hPI(3)K (mAb p110) WB (anti-human Phosphoinositide 3-kinase gamma) mouse monoclonal antibody, IgG2a, clone H1 -hPI(3)K (mAb p110) WB (anti-human Phosphoinositide 3-kinase gamma) mouse monoclonal antibody, IgG2a, clone H1 -hPI(3)K (mAb p110) IP (anti-human Phosphoinositide 3-kinase gamma) mouse monoclonal antibody, IgG2a, clone 641 -hPI(3)K (mAb p110) IP (anti-human Phosphoinositide 3-kinase gamma) mouse monoclonal antibody, IgG2a, clone 641 Qty 200 l
INH-002 INH-003 INH-004 INH-005
1 ml
1 ml
100 g
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105
Protein Kinases Tyrosine & Ser/Thr Kinases

Tyrosine Kinases (Contd.)
N-terminal GST tagged Abl SH3 domain. Abl SH3 domain is the SH3 domain of Abl tyrosine kinase. SH3 domains are known to bind proline rich peptides. The Abl SH3 domain has been expressed as a fusion protein with GST allowing expression in E. coli and purification by affinity chromatography. N-terminal GST tagged Hck SH3 domain. Hck SH3 domain is the SH3 domain of Hck tyrosine kinase. SH3 domains are known to bind proline rich peptides. The Hck SH3 domain has been expressed as a fusion protein with GST allowing expression in E. coli and purification by affinity chromatography. Src (p60c-src) is a 60 kDa cytoplasmic tyrosine kinase with functions in the regulation of cell division, cell-matrix adhesion, and cell-cellcell adhesion. Src family tyrosine kinases participate in signal transduction of different classes of membrane receptors, including receptor tyrosine kinases and G-protein coupled receptors. N-terminal GST tagged Src SH3 domain. Src SH3 domain is the SH3 domain of Src tyrosine kinase. SH3 protein with GST allowing expression in E. coli and purification by affinity chromatography. Platelet-derived growth factors (PDGF) are potent mitogens for cells of mesenchymal origin, including smooth muscle cells and glial cells. The PDGF isoforms AA, BB, AB, CC, and DD are dimeric molecules. They bind two receptor molecules simultaneously and dimerize them upon binding. The autophosphorylation induced after dimerization of PDGF receptors serves two important functions. On one hand, phosphorylation of a conserved tyrosine residue inside the kinase domains (Tyr-849 in the receptor and Tyr-857 in the -receptor) leads to an increase in the catalytic efficiencies of the kinase. On the other hand, autophosphorylation of tyrosine residues located outside the kinase domain creates docking sites for signal transduction molecules containing SH2 domains. Cat. No. PR-305 Product
SIGNAL TRANSDUCTION
PTK, PKA, PKB, PKC
Qty 50 g
RafRBD (c-Raf)(Ras Binding Domain of Raf, c-Raf 1 Kinase) RafRBDGST (c-Raf)(Ras Binding Domain of Raf, c-Raf 1 Kinase)
PR-366
50 g
Serine/Threonine Kinases
MKK6 is one of the major specific activators of p38. MAP kinase kinases (MAPKKs) MKK3 and MKK6 are the primary regulators of p38 phosphorylation and activation. The MKK6/p38 pathway is well documented in the regulation of apoptosis. The p38 mitogen-activated protein (MAP) kinase signal transduction pathway regulates the production of interleukin1 and tumor necrosis factor-alpha. The recombinant human MKK6 was expressed in E. coli and purified by glutathione sepharose chromatography. MAP (mitogen-activated protein) kinases are activated by a family of dual specificity kinases called MEKs (MAP kinase/Erk Kinase). MEK1 can be activated by Raf by phosphorylation on serine 218 and serine 222. Mutation of these sites to acidic residues leads to constitutively active MEK1 in some cases. Given the central role of the ERK/mitogen-activated protein kinase pathway in mediating growth-promoting signals for a diverse group of upstream stimuli, inhibitors of MEK, as a key central mediator, could have significant clinical benefit in the treatment of breast and other cancers. The human recombinant MEK1, activated by two amino acid exchanges (S218D, S222D), was expressed in E. coli and purified by gluthatione sepharose and gelfiltration. It is suitable for labeling MEK1 substrates and for activation of ERK1 and ERK2. The molecular weight of the protein is 69.7 kDa.
Cat. No. Cat. No. PR-337 Product AblGST SH3 domain (Abelson Tyrosine Kinase, SH3 Domain) HckGST SH3 domain (Hematopoietic Tyrosine Kinase, SH3 domain) LckGST SH3 domain (Lymphocyte-specific Kinase, SH3 Domain) Src GST (Sarcoma inducing protein kinase) SrcGST SH3 domain (Sarcoma inducing tyrosine kinase, SH3 domain) PDGFR-GST, cytosolic region (Platelet-derived growth factor receptor) Qty 50 g PR-161 PR-336 50 g PR-321
Product MKK6GST, active(Mitogenactivated protein kinase kinase) MEK1GST, active(Mitogen-activated kinase kinase 1, MAPKK1)
Qty 20 g
10 g
PR-339
50 g
Protein A, B and C Kinases

The principle intracellular target for cAMP in mammalian cells is the cAMP-dependent protein kinase (PKA). A large number of hormones, neurotransmitters and other signal substances utilize adenosine 3, 5 cyclic monophosphate (cAMP) as an intracellular second messenger. The recombinant human PKA, catalytic subunit alpha was expressed in E. coli and purified by Ni-agarose chromatography. It is suitable for labeling PKA substrates. The sequence based calculated molecular weight is 42.3 kDa. Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB (Akt1), PKB (Akt2), and PKB (Akt3). PKB/Akt is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components. The Thr-308 residue in the kinase domain and Ser-473 residue in the tail domain of Akt1 need to be phosphorylated by PDK1 and PDK2, respectively, for its maximal activation. The activation mechanism remains to be fully characterised but occurs downstream of PI-3K. Akt1 contains a region homologous to a pleckstrin domain found in multiple signaling molecules and is stimulated by a number of receptor tyrosine kinases, including receptors for IGF, NGF, PDGF, VEGF, angiotensin, and insulin, by the action of PI 3-kinase. The recombinant human Akt1 kinase (PKB) is expressed in Sf9 insect cells and purified by Ni-NTA agarose chromatography. It is suitable for labeling Akt1 kinase substrates. The sequence based calculated molecular weight is 59.05 kDa.
PR-900
20 g
PR-338
50 g
PR-357
20 g
Protein Kinases - Tyrosine Like Kinases

Raf is a member of the serine/threonine proteine kinase family involved in regulation of cell growth and differentiation and is the most important effector of Ras. Full-length Raf is composed of three conserved regions responsible for interaction with Ras, for phosphorylation and for catalytic activity. RafRBD (Ras binding domain, amino acids 50-132) mediates interaction with membrane-anchored Ras necessary for activation of the kinase activity of Raf.
106
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Protein Kinases PKA, PKB, PKC, CamK, Cyclin dependent

Protein A, B and C Kinases
Protein kinase C alpha (PKC alpha) is a serine/threonine kinase and a member of the conventional (classical) PKCs (cPKCs), which have four conserved (C1 to C4) regions. This ubiquitously expressed PKC isotype is activated in response to many different kinds of stimuli and translocates from cytosol to the specialized cellular compartments (nucleus, focal adhesion, caveolae, etc.) where it is presumed to work. Therefore, PKC alpha has been implicated in a variety of cellular functions including proliferation, apoptosis, differentiation, motility, and inflammation. The recombinant human PKCwas expressed in E coli and purified by Niagarose chromatography. It is suitable for labeling PKC substrates. The sequence based calculated molecular weight is 81.9 kDa.
SIGNAL TRANSDUCTION
PKA, PKB, PKC, CamK, Cyclin dep.
Cyclin-dependent Kinases
Casein kinase 2 (CK2) is a ubiquitous pleiotropic proliferation-associated serine/threonine protein kinase. Similar to protein kinase A, CK2 is a tetramer made up from two different subunits 22. The catalytic subunit corresponds to the C-subunit of PKA, the non-catalytic -subunit is unique and differs from the R-subunit of PKA in all known features. The recombinant human CK2 subunits and were expressed separately as non-fusion proteins in E. coli and reconstituted to the 22 holoenzyme in the course of the purification using several chromatography steps. The protein is suitable for labeling CK2 substrates. The molecular weight is 140 kDa. The human recombinant protein kinase CK21 was expressed in E. coli as a non-fusion protein and purified by several chromatography steps. The -subunit is constitutively active and suitable for labeling casein kinase 2 alpha substrates. The human recombinant protein kinase CK22 was expressed in E. coli as a N-MBP fusion protein. The -subunit is constitutively active and suitable for labeling casein kinase 2 alpha substrates. The molecular weight of the protein is 84.3 kDa. JNK2 2/SAPK1a belongs to the family of mitogenactivated protein kinases (MAPK). Mitogen-activated protein (MAP) kinases comprise a family of ubiquitous proline-directed, protein serine/ threonine kinases, which participate in signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. p38 /SAPK2a belongs to the family of mitogen-activated protein kinases (MAPK). Mitogen-activated protein (MAP) kinases comprise a family of ubiquitous proline-directed, proteinserine/ threonine kinases, which participate in signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. The recombinant human p38kinase (SAPK2a) was expressed in E. coli and purified by Ni-agarose chromatography. The active form was produced by phosphorylation of the purified p38with MKK6. It is suitable for labeling p38kinase substrates. The sequence based calculated molecular weight is 42.7 kDa. MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. At least three MAPK families have been characterized: extracellular signalregulated kinase (ERK), Jun kinase (JNK/SAPK) and p38 MAPK. Activated ERK can enter the nucleus and phosphorylate transcription factors providing the link between cell surface receptor-mediated events and nuclear induction of gene expression. In the nucleus activated ERK promotes the transcription and the activity of transcription factors cfos, c-myc, c-jun and p21. The recombinant human ERK2 was expressed in E. coli and purified by Ni-agarose chromatography. It is suitable for labeling ERK2/MAPK2 substrates. The highly active form is produced by phosphorylation of the protein in vitro with MEK1. The sequence based calculated molecular weight is 44.56 kDa. The recombinant human ERK1 was expressed in E. coli and purified by Ni-agarose chromatography. It is suitable for labeling ERK1 substrates. The highly active form is produced by phosphorylation of the protein in vitro with MEK1. The sequence based calculated molecular weight is 43.62 kDa. Cat. No. PR-316 Product CK2, holoenzyme, active (Casein Kinase 2, - and -subunit) Qty 10 g
Cat. No. PR-318 PR-324
Product PKAHis, -subunit, active (Protein Kinase A) Akt1/ PKBHis, active (Protein Kinase B) Akt1/ PKBHis, inactive (Protein Kinase B) Akt2/PKB His, active (Protein kinase B) Akt3/ PKB His, active (Protein Kinase B) PKCHis, active (Protein Kinase C)
Qty 50 g 20 g
PR-325 PR-333 PR-334
20 g 100 g 20 g
PR-329
10 g
Calcium/Calmodulin-dependent Kinases
Checkpoint kinase 2 (CHK2) is emerging as a key mediator of diverse cellular responses to genotoxic stress, guarding the integrity of the genome throughout eukaryotic evolution. Recent studies show the fundamental role of CHK2 in the network of genome surveillance pathways that coordinate cell-cycle progression with DNA repair and cell survival or death. Defects in CHK2 contribute to the development of both hereditary and sporadic human cancers, and earmark this kinase as a candidate tumour suppressor and an attractive target for drug discovery. The recombinant human CHK2 was expressed in Sf9 insect cells and purified by Ni-NTA agarose chromatography. It is suitable for labeling CHK substrates. The sequence based calculated molecular weight is 66.06 kDa. Cat. No. PR-313 Product CHK2His, active (Checkpoint Kinase 2) Qty 20 g
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Protein Kinases Cyclin dependent, Substrates, Inhibitors

Cyclin-dependent Kinases (Contd.)
Cat. No. PR-314 PR-160 PR-317 PR-315 PR-326 PR-327 PR-328 PR-331 Product CK21, active (Casein Kinase 2, -subunit) CK22MBP, active (Casein Kinase 2, -prime subunit) CK2, active (Casein Kinase 2, -subunit) CK2 (Casein Kinase 2, -subunit) JNK22/ SAPK1a His, active (c-jun kinase/ stress-activated protein kinase) p38/ SAPK2aHis, active (Stress-activated protein kinase) p38/ SAPK2aHis, inactive (Stress-activated protein kinase) p42/ ERK2/ MAPK2His, active (Extracellular signal-regulated kinase/ mitogen-activated protein kinase) p42/ ERK2/ MAPK2His, inactive (Extracellular signal-regulated kinase/ mitogen-activated protein kinase) p42/ ERK2/ MAPK2His (Extracellular signal-regulated Kinase) Rat, Recombinant, E. coli p44/ERK1His, active (Extracellular signal-regulated kinase/ mitogen-activated protein kinase) p44/ERK1His, inactive (Extracellular signal-regulated kinase/ mitogen-activated protein kinase) Qty 10 g 10 g 10 g 10 g PE-204 100 g PE-205 20 g PE-206 20 g 5 g PE-207
SIGNAL TRANSDUCTION
Cyclin dep. Kinase, substrate, Inhibitors
Substrate Peptides
Cat. No. PE-200 Product Akt substrate peptide RPRAATF Abl substrate peptide EAIYAAPFAKKK Cdc2 substrate peptide (Ac-S)PGRRRRK Cdk5 substrate peptide PKTPKKAKKL CHK substrate peptide KKKVSRSGLYRSPSMPENLNRPR CK1 substrate peptide RRKDLHDDEEDEAMSITA CK2 substrate peptide 1 RRRDDDSDDD CK2 substrate peptide 2 RRRADDSDDDDD GSK3 substrate peptide YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE PKA substrate peptide GRTGRRNSI PKC substrate peptide QKRPSQRSKYL Src substrate peptide KVEKIGEGTYGVVYK PE-200 AMPK substrate peptide HMRSAMSGLHLVKRR DYRK substrate peptide KKISGRLSPIMTEQ LKB1 substrate peptide LSNLYHQGKFLQTFCGSPLYRRR Qty 1 mg
PE-201
1 mg
PE-203
1 mg
1 mg
1 mg
1 mg
1 mg
PE-213 10 g PE-202 50 g PE-208 10 g
1 mg
PR-332
1 mg
PR-320
1 mg
PR-322
PE-209
1 mg
PR-323
20 g
1 mg
1 mg
Inhibitors
Cat. No. INH-003 INH-004 INH-005 Product Quercetin (Protein Kinase Inhibitor) Myricetin (Protein Kinase Inhibitor) Staurosporine (Protein Kinase Inhibitor) Streptomyces staurosporeus SP600125 (JNK Inhibitor) SB203580 (p38 MAPK Inhibitor) SB202190 (p38 MAPK Inhibitor) Qty 100 mg 5 mg 50 g
PE-201
1 mg
PE-203
1 mg
PKA Substrates
Cat. No. 60510-1 Product CREBtide Native KRREILSRRPSYR (Also available in Fluor & Biotin derivatives) Kemptide Native LRRASLG Kemptide Phosphorylated LRRAS(PO3)LG (Also available in Fluor & Biotin derivatives) Qty 1 mg
INH-006 INH-007
2 mg 2 mg
22593 60528-1
1 mg 1 mg
INH-008
2 mg
PKA, PKC, MAPKAP-K1 Substrates

Cat. No. 29983-1 Product Native RRRLSSLRA-NH2 (Also available in Fluor & Biotin derivatives) Qty 1 mg
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Substrates for Protein Kinases

PKC Substrates
Cat. No. 27165 Product MARCKS Protein (154-165) Native KKRFSFKKSFKL (Also available in Fluor & Biotin derivatives) Neurogranin 28-43 Native AAKIQASFRGHMARKK Neurogranin 28-43 Phosphorylated AAKIQAS(PO3)FRGHMARKK (Also available in Fluor & Biotin derivatives) Glycogen Synthase residues 1-8 Native PLSRTLSVAAKK-NH2 (Also available in Fluor & Biotin derivatives) RRGRTGRGRRGIFR (Native) (Also available in Fluor & Biotin derivatives) RRGRTGRGRRGIFR Phosphorylated Native RFAVRDMRQTVAVGVIKAVDKK Phosphorylated RFAVRDMRQT(PO3)VAVGVIKAVDKK (Also available in Fluor & Biotin derivatives) Qty 1 mg
SIGNAL TRANSDUCTION
Kinase Substrates
Substrates for Enzyme CaMK

Cat. No. 60249-1 29929-1 Product Autocamtide-2 Native KKALRRQETVDAL Autocamtide-2 Phosphorylated KKALRRQET(PO3)VDAL (Also available in Fluor & Biotin derivatives) Autocamtide-3 Native KKALHRQETVDAL Autocamtide-3 Phosphorylated KKALHRQET(PO3)VDAL (Also available in Fluor & Biotin derivatives) Qty 1 mg 1 mg
27173
1 mg
29994-1
1 mg
29973-1
1 mg
27177
1 mg
29974-1
1 mg
60542-1
1 mg
Substrates for CaM Kinase II

1 mg 1 mg 1 mg Cat. No. 29954-1 Product Residues 281-291 of CamK II (autophophorylation site) Native MHRQETVDCLK-NH2 Residues 281-291 of CamK II Phosphorylated MHRQET(PO3)VDCLK-NH2 (Also available in Fluor & Biotin derivatives) Glycogen Synthase residues 1-8 Native PLSRTLSVAAKK-NH2 (Also available in Fluor & Biotin derivatives) Glycogen Synthase residues 1-10 Native PLSRTLSVSS-NH2 (Also available in Fluor & Biotin derivatives) Syntide-2 Native PLARTLSVAGLPGKK (Also available in Fluor & Biotin derivatives) Qty 1 mg
60546-1 60560-1 60535-1
29958-1
1 mg
PKC Substrates
Cat. No. 29924-1 29928-1 Product Native AALVRQMSVAFFFK Phosphorylated AALVRQMS(PO3)VAFFFK (Also available in Fluor & Biotin derivatives) Qty 1 mg 1 mg 60514-1 27177
1 mg
1 mg
PKC Substrate
Cat. No. 27183 60533-1 Product Native ERMRPRKRQGSVRRRV Phosphorylated ERMRPRKRQGS(PO3)VRRRV (Also available in Fluor & Biotin derivatives) Qty 1 mg 1 mg
22511
1 mg
Substrate for AKT/PKB

Cat. No. 60209-1 Product Crosstide [GRPRTSSFAEG] Native (Also available in Fluor & Biotin derivatives) ARKRERTYSFGHHA Native (Also available in Fluor & Biotin derivatives) ARKRERTYS(PO3) FGHHA Phoshphorylated Qty 1 mg 1 mg
PKG Substrates
Cat. No. 60506-1 Product BPDEtide [RKISASEFDRPLR] Native RKISASEFDRPLR (Also available in Fluor & Biotin derivatives) Native QKRPRRKDTP Phosphorylated QKRPRRKDT(PO3)P (Also available in Fluor & Biotin derivatives) Native RKRSRAE Phosphorylated RKRS(PO3)RAE (Also available in Fluor & Biotin derivatives) Qty 1 mg
29944-1
29946-1 1 mg 1 mg
1 mg
27185 29997-1
60298-1 60302-1
1 mg 1 mg
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Substrate for Protein Kinases & Phosphatases

Substrates for MAPKs
Cat. No. 27168 Product Native APRTPGGRR Substrate fopr p44 MAPK (ERK1) & p42 MAPK (ERK-2) Phosphorylated APRT(PO3)PGGRR (Also available in Fluor & Biotin derivatives) EGF Receptor 661-681 Native KRELVEPLTPSGEAPNQALLR-NH2 EGF Receptor 661-681 Phosphorylated KRELVEPLT(PO3)PSGEAPNQALLR-NH2 (Also available in Fluor & Biotin derivatives) Tyrosine Hydroxylase 24-33 Native KQAEAVTSPR (Also available in Fluor & Biotin derivatives) Qty 1 mg
SIGNAL TRANSDUCTION
Kinase Substrates
Substrates for Protein Tyrosine Kinase

Cat. No. 29942-1 29937-1 Product Native ADEYLIPQQ Phosphorylated ADEY(PO3)LIPQQ (Also available in Fluor & Biotin derivatives) Tyrosine Kinase Peptide 1 KVEKIGEGTYGVVYK (Native) (Also available in Fluor & Biotin derivatives) Tyrosine Kinase Peptide 3 RRLIEDAEYAARG (Native) (Also available in Fluor & Biotin derivatives) Qty 1 mg 1 mg
60746
1 mg
60551-1
1 mg
29969-1
1 mg 22581 1 mg
1 mg
29965-1
Substrates for Protein Tyrosine phosphatase-1B

1 mg Cat. No. 60634 Product Phosphorylated Ac-ELEFY(PO3)MDYE-NH2 (Also available in Fluor & Biotin derivatives) EGF Receptor Substrate 2 Phosphorylated DADEY(PO3)LIPQQG (Also available in Fluor & Biotin derivatives) Native ELEFY(PO3)MDYE-NH2 (Also available in Fluor & Biotin derivatives) Qty 1 mg
60294-1
Substrate for Calcineurin (PP2B)

Cat. No. 24516 Product Phosphorylated DLDVPIPGRFDRRVS(PO3)VAAE (Also available in Fluor & Biotin derivatives) Qty 1 mg
24433
1 mg
29950-1
1 mg
Substrates for p60c-src

Cat. No. 27197 Product Native YIYGSFK (Also available in Fluor & Biotin derivatives) Qty 1 mg
Substrate for CDK5

Cat. No. 60026-1 Product Native PKTPKKAKKL (Also available in Fluor & Biotin derivatives) Phosphorylated PKT (PO3) PKKAKKL Qty 1 mg
Substrate for p43 (cd2)

Cat. No. 22579 Product Native ADAQHATPPKKKRKVEDPKDF (Also available in Fluor & Biotin derivatives) Phosphorylated ADAQHAT (PO3)PPKKKRKVEDPKDF Qty 1 mg
29982-1
1 mg
Substrate for CDK1

Cat. No. 60522-1 Product Native HATPPKKKRK (Also available in Fluor & Biotin derivatives) Phosphorylated HAT(PO3)PPKKKRK Qty 1 mg
29923-1
1 mg 60526-1
1 mg
Substrates for Protein Tyrosine phosphatases

Cat. No. 24526 Product Protein Tyrosine Phosphatase Substrate I Native ENDY(PO3)INASL (Also available in Fluor & Biotin derivatives) MCA-EDAEY (PO3)AAK(DNP)R-NH2 FRET substrate for assaying PTPs Qty 1 mg
Substrates for Enzyme Casein Kinase

Cat. No. 60537-1 60541-1 Product Native RRADDSDDDDD Substrate for CKII Phosphorylated RRADDS(PO3)DDDDD (Also available in Fluor & Biotin derivatives) Native RRKDLHDDEEDEAMSITA (Also available in Fluor & Biotin derivatives) Qty 1 mg 1 mg
60512-1
1 mg 60547-1
1 mg
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Phosphatases
Recombinant Phosphatases
The dual specific Cdc25A (Cell division cycle) phosphatase regulates cell cycle progression, has oncogenic and anti-apoptotic activity, and is overexpressed in many human tumors. Protein Tyrosine Phosphatase type IVA, member 3, isoform 1; whole sequence. The proteins of regenerating liver (PRL-1, -2, and -3) are closely related protein tyrosine phosphatases (PTPs) having high sequence similarities to several dual-specificity PTPs. Elevated expression levels of PRL-3 have been reported in colon cancer cells that have metastasized to liver. PTEN is one of the most frequently mutated tumor suppressors in human cancer. PTEN is a dual-specificity phosphatase with both protein phosphatase and lipid phosphatase activity. The best described substrate of PTEN is phosphatidylinositol (3,4,5)tris-phosphate (PtdIns(3,4,5)P3). PTEN removes the phosphate in PtdIns(3,4,5)P3 to generate PtdIns(4,5)P2 and serves to counter-balance the effects of PI3 Kinase. PTEN regulate the signaling pathway of PI3 Kinases by preventing localisation of proteins with pleckstrin homology domains to the cell membrane. The molecular weight is about 48 kDa. PTP 1B is the prototype non-transmembrane protein tyrosine phosphatase. It is a ubiquitous protein that was originally identified in human placenta. This highly active enzyme is well suited for the study of tyrosine phosphatase kinetics and substrate specificity, as well as for the screening of inhibitors. PTP-SL (PTPBR7) is a cytosolic membrane-associated enzyme with a single catalytic domain. PTP-SL is a major regulator of extracellular signal-regulated kinase ERK1/2 and p38. Interaction between PTP-SL and ERK1/2 and p38 is strictly dependent on a kinase interaction motif (KIM) consisting of 16 amino acid residues. PR-319 is is the catalytically inactive form of PTP-SL-ex, which was obtained by site-directed mutagenesis of the active site cysteine to serine. The intracellular region of PTP contains three domains: the juxtamembrane (JM, aa 765-923) and two phosphatase domains (D1 and D2). Only the D1 domain is catalytically active. Protein Tyrosin Phosphatase, cytoplasmic region, containing catalytic domain D1 C-terminally linked to the non-catalytic domain D2. Molecular weight is 72.95 kDa. SHP-1 is a protein-tyrosine phosphatase with tandem SH2 domains. It is a negative regulator of signaling of various receptors including the erythropoietin receptor, the IL-3 receptor, c-Kit, the CSF-1 receptor, the Bcell receptor and c-Ros. The mammalian dual-specificity protein-tyrosine phosphatase VHR (for VH1-related) has been identified as a novel regulator of extracellular regulated kinases (ERKs). Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes phosphate groups from tyrosine, serine, and threonine residues. It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to act as a phosphatase for several members of the MAP kinase family including ERK1, ERK2, and JNK. It is a target for the ZAP-70 kinase, and phosphorylation of VHR at tyrosine 138 leads to a downregulation of ERK2 activity. Protein Tyrosine Phosphatase-Basophil-like (PTP-BL)is a large nonreceptor type protein tyrosine phosphatase caracterized by the presence of a extreme N-terminal KIND domain (kinase noncatalytic C-lobe domain), a N-terminal FERM domain (four point one, ezrin, radixin, moesin homology domain), five PDZ (postsynaptic density protein-95, discs large, zonula occludens) regions and a C-terminal catalytically active domain. Cat. No. PR-348 PR-330 Product
SIGNAL TRANSDUCTION
Phosphatases
Qty 20 g 20 g
Cdc25A (Cell division cycle phosphatase) Human PRL-3 (Protein Tyrosine Phosphatase with C-terminal Prenylation Motif, Phosphatase of Regenerating Liver)Human PTEN (phosphatase and tensin homologue deleted on chromosome 10) human PTP 1B (Protein Tyrosine Phosphatase 1B) Human PTP-SL-cat (Protein Tyrosine Phosphatase, Catalytic Domain) Mouse PTP-SL-ex (Protein Tyrosine Phosphatase) Mouse PTP-SL-ex, catalytically inactive mutant (Protein tyrosine phosphatase) mouse PTP-SL-kim (Protein Tyrosine Phosphatase, Kinase Interaction Motif and Catalytic Domain) Mouse PTP, catalytic domain D1(N2) (Protein tyrosine phosphatase) Human PTP, cytoplasmic region (D1D2) (Protein tyrosine phosphatase) Human SHP-1 GST (Src homology 2 domain Phosphatase) Human VHR (VH1-related phosphatase) Human PTP-BL, catalytic domain (Protein tyrosine phosphatase Basophil-like) mouse
PR-930
5 g
PR-931 PR-311
20 g 20 g
PR-310 PR-319 PR-312
20 g 20 g 20 g
PR-309 PR-349 PR-901
20 g 20 g 20 g
PR-350 PR-308
20 g 20 g
Phosphatase Assay Kit

Protein phosphatases consist of three general families with different substrate specificities towards phospho-seryl/threonyl, -tyrosyl residues, or both. This kit provides a simple means to measure phosphatase activity by using pNPP as a general substrate of phosphatases. In a first step, the enzyme dephosphorylates its substrate pNPP. In a second step, the phenolic OH-group is deprotonated under alkaline conditions resulting in p-nitrophenolate with a strong absorption at 405 nm (yellow). Cat. No. AK-101 Product Phosphatase Assay Kit (pNPP-based colorimetric assay, 96 well format) Qty 1 Kit
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Assay Kits for Protein Phosphorylation

EnzoLyte Serine/Threonine Protein Kinase Substrate Sampler Kit *FAM-Labeled*
The EnzoLyte Serine Protein Kinase Substrate Sampler Kit provides ten FAM-labeled protein kinase substrates along with two phosphorylated peptide controls. These substrates can be utilized to design fluorescence polarization-based kinase assays, facilitating the identification of an appropriate peptide substrate for the serine kinase of interest. Cat. No. 71202 Product EnzoLyte Serine/Threonine Protein Kinase Substrate Sampler Kit *FAM-Labeled* Qty 1 kit
SIGNAL TRANSDUCTION
Kinase & Phosphatase Assay kits
EnzoLyte MFP Protein Phosphatase Assay Kit *Fluorimetric*

MFP is a proprietary fluorogenic substrate for most phosphatases, i.e., alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The phosphatase-induced hydrolysis of MFP yields an intense fluorescent product that has excitation wavelength around 488 nm, and emission maximum around 520 nm. EnzoLyte FMP Protein Phosphatase Assay Kit uses MFP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. Cat. No. 71104 Product EnzoLyte MFP Protein Phosphatase Assay Kit *Fluorimetric* Qty 500 assays
EnzoLyte Tyrosine Protein Kinase Substrate Profiling Kit *Fluorimetric*

EnzoLyte Tyrosine Protein Kinase (TPK) Substrate Sampler Kit is designed to facilitate the identification of the optimum peptide substrate for developing a particular TPK assay. The EnzoLyte Tyrosine Protein Kinase Substrate Profiling Kit provides four FAM-labeled protein kinase substrates, four biotin-labeled substrates, and two phosphorylated peptide controls. Cat. No. 71203 Product EnzoLyte Tyrosine Protein Kinase Substrate Profiling Kit *Fluorimetric* Qty 1 kit
The kit contains: MFP phosphatase substrate Assay buffer
EnzoLyte MG Phosphate Assay Kit *Colorimetric*

EnzoLyte MG Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate on a spectrophotometer (600-660 nm) or on a plate reader. Assays can be performed in tubes, cuvettes or multi-well plates and can detect as little as 1.6 pmoles of phosphate (detection range between 0.02 mM and 40 mM phosphate). The kit has been used for assaying phosphatase, phospholipase or lipase, nucleoside triphosphatase (ATPase, GTPase etc) and phytase. Cat. No. 71103 Product EnzoLyte MG Phosphate Assay Kit *Colorimetric* Qty 1000 assays
The kit contains: Four FAM-labeled TPK substrates Four Biotin-labeled TPK substrates Two phosphorylated peptide controls.
EnzoLyte Protein Kinase Substrate Sampler Kit *Biotinylated*

EnzoLyte Tyrosine Protein Kinase (TPK) Substrate Sampler Kit *Biotinylated* is designed to facilitate the characterization of an appropriate peptide substrate for developing a particular TPK assay. The kit contains 10 popular biotinylated protein kinase substrates along with two phosphorylated peptide controls. It provides a convenient solution for identifying a suitable substrate for designing biotinylated peptide-based protein kinase assays. Cat. No. 71200 Product EnzoLyte Protein Kinase Substrate Sampler Kit *Biotinylated* Qty 10 x 500 assays
Contents: Highly purified Malachite Green reagent, Assay buffer
EnzoLyte pNPP Protein Phosphatase Assay Kit *Colorimetric*

p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for protein tyrosine phosphatases and serine/threonine phosphatases. The EnzoLyte pNPP Protein Phosphatase Assay Kit uses pNPP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. It has high sensitivity and wide linear range (The detection limit is generally 3 ng or below). Cat. No. 71105 Product EnzoLyte pNPP Protein Phosphatase Assay Kit *Colorimetric* Qty 500 assays
EnzoLyte Protein Kinase Substrate Sampler Kit *FAM-labeled*

EnzoLyte Tyrosine Protein Kinase (TPK) Substrate Sampler Kit *FAMLabeled* is designed to facilitate the characterization of an appropriate peptide substrate for developing a particular TPK assay. The kit contains 10 FAM-labeled protein kinase substrates along with two phosphorylated peptide controls. It provides a convenient solution for identifying a suitable substrate for designing FAM-labeled peptide-based protein kinase assays. Cat. No. 71201 Product EnzoLyte Protein Kinase Substrate Sampler Kit *FAM-labeled* Qty 10 x 500 assays
Contents: pNPP chromogenic substrate, Assay buffer
Fluorescein diphosphate, tetraammonium salt (FDP)

Fluorescein diphosphate (FDP) is one of the most sensitive fluorogenic phosphatase substrates. The colorless and nonfluorescent FDP is hydrolyzed to highly fluorescent fluorescein. Cat. No. 85300 Product Fluorescein diphosphate, tetraammonium salt (FDP) Qty 5 mg
Antibodies
We have a wide range of Antibodies for signaing related proteins. Please enquire.
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Antibodies and related products

We offer more than 11,000 monoclonal and polyclonal antibodies for research and diagnosis and a vast number of secondary antibodies labeled to AP, Biotin, Colloidal Gold, Cyan, FITC, HRP, PE, Texas Red and TRITC are available. Also available are the antigens. Primary Antibodies Secondary Antibody Conjugates Epitope Tags, Fusion Proteins etc. Detection Kits Enzyme Substrates Avidin, Biotin, Streptavidin RIA-Kits Protein Standards & Controls Custom Antibody Production and Services
ANTIBODIES
Products
Secondary Antibodies
Cat. No. 28164 28165 28166 28167 28168 28169 28170 28171 28172 28173 28174 28175 28176 28177 28178 Product Rabbit Anti-Mouse IgG (H+L), purified Rabbit Anti-Mouse IgG (H+L), HRP-Conjugated Rabbit Anti-Mouse IgG (H+L), AP-Conjugated Rabbit Anti-Mouse IgG (H+L), Biotinylated Rabbit Anti-Goat IgG (H+L), purified Rabbit Anti-Goat IgG (H+L), HRP-Conjugated Rabbit Anti-Goat IgG (H+L), AP-Conjugated Rabbit Anti-Goat IgG (H+L), Biotinylated Goat Anti-Mouse IgG (H+L), purified Goat Anti-Mouse IgG (H+L), HRP-Conjugated Goat Anti-Mouse IgG (H+L), AP-Conjugated Goat Anti-Mouse IgG (H+L), Biotinylated Goat Anti-Rabbit IgG (H+L), purified Goat Anti-Rabbit IgG (H+L), HRP-Conjugated Goat Anti-Rabbit IgG (H+L), AP-Conjugated Goat Anti-Rabbit IgG (H+L), Biotinylated Rabbit Anti-Chicken IgY, purified Rabbit Anti-Chicken IgY, HRP-Conjugated Rabbit Anti-Chicken IgY, AP-Conjugated Rabbit anti-Chicken IgY, Biotinylated Qty 1 mg 0.5 mg 0.5 mg 0.5 mg 1 mg 0.5 mg 0.5 mg 0.5 mg 1 mg 0.5 mg 0.5 mg 0.5 mg 1 mg 0.5 mg 0.5 mg 0.5 mg 1 mg 0.5 mg 0.5 mg 0.5 mg
ELISA Substrates
OPD Fizzing Tablets TMB Fizzing Tablets
Other substrates also available
WB/IHC Substrate
AEC Fizzing Tablets FAST RED/ Naphtol Urea Peroxyde Tablets BCIP / INT p-NPP NBT/ ROCK TMB
28179 29709 29710 29711 29712
Immunoblotting and Immunhistochemistry Substates

BCIP/ NBT BCIP/ TNBT DAB TMB Blotting
Epitope Tag antibodies

Cat. No. 29774 29779 29674 29629 29673 61250 29673-BIOT 29673-FITC Product Anti-C-Myc Tag Anti-GFP Anti-DYKDDDDK Anti-HA Tag Anti-His Tag, rabbit polyclonal Anti-His Tag IgG, mouse monoclonal Rabbit Polyclonal Anti-His Tag IgG, Biotinylated Rabbit Polyclonal Anti-His Tag IgG, FITClabeled, Also available in other Fluorescent tags like FAM, AMCA, HiLyte Fluor ,TAMRA Mouse Monoclonal Anti-His Tag IgG, FITClabeled, Also available in other Fluorescent tags like FAM, AMCA, HiLyte Fluor ,TAMRA Mouse Monoclonal Anti-His Tag IgG, Biotinylated Monoclonal Anti-GST Anti-GST Tag, Mouse Monoclonal, Biotinylated Qty 100 g 100 g 100 g 100 g 100 ug 100 ug 100 g 100 g
Secondary Antibodies
Alkaline Phosphatase Horse Radish Peroxidase Biotin (BAC) Conjugates Cyanine (CYTM) Conjugates DiI-AC-LDL / DiI-LDL Di0 LDL F (ab) Fragments and F (ab) 2 Fragments of Antibodies Gold Labelled Secondary Antibodies and F (ab) 2 Fragments Fluorescein Conjugates (FITC) Peroxide Anti-Peroxidase (PAP) Phycocyanin and Allophycocyanin Phycoerythrin Conjugates Rhodamine and Texas RedTM Conjugates
61250-FITC
100 g
61250-BIOT 29531 29531-BIOT 29531-FITC
100 g 100 g 100 ug
Anti-GST Tag, Mouse Monoclonal, FITC labeled; 100 g Also available in other Fluorescent tags like FAM, TAMRA, AMCA, HiLyte Fluor
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113
Biotin Derivatives, Avidin Conjugates

AnaTag Protein Biotinylation Kit
It includes all essential components for biotinylation and purification with a detailed operation protocol. Cat. No. 71003 Product AnaTag Protein Biotinylation Kit Qty 1 kit
ANTIBODIES
Biotin-Avidin
Amino-containing Biotins for Modifying Carbohydrates, Carbonyl, Carboxyl Groups

Cat. No. 60647 60648 Product Biocytin hydrazide More polar than biotin; used for cell tracing Biotin cadaverine [N-(5-Aminopentyl) biotinamide, trifluoroacetic acid salt] Building block for modifying carboxy and carbonyl groups; substrate for transglutaminase Biotin ethylenediamine [N-(2-Aminoethyl) biotinamide, hydrobromide] Building block for modifying carboxy and carbonyl groups Biotin hydrazide Biotinylating reagent for carbohydrates and carbonyl compounds Biotin-X cadaverine [5-(((N-(Biotinoyl)amino) hexanoyl)amino) pentylamine, trifluoroacetic acid salt] Building block for modifying carboxy and carbonyl groups; substrate for transglutaminase; longer spacer than 60648 Biotin-XX hydrazide [6-((6 ((Biotinoyl)amino)hexanoyl)amino) hexanoic acid, hydrazide] Biotinylating reagent for carbohydrates and carbonyl compounds; better avidin-binding due to the longer spacer FMOC biocytin [e-Biotinoyl-a-(9 fluorenylmethoxycarbonyl)-L-lysine] Building block for preparing biotinylated peptides Qty 25 mg 25 mg
Amino-Reactive Biotins
Cat. No. 60638 21100 60639 21109 Product Biocytin, [e-Biotinoyl-L-lysine] More polar than biotin; used for cell tracing D-Biotin Highly purified and tested for avidin-binding D-Biotin *100 mM aqueous solution* Ready-to-use solution D-Biotin, SE [Succinimidyl D-biotin] Popular biotinylating reagent for nucleotides, peptides and proteins Biotin-X, SE [6-((Biotinoyl)amino) hexanoic acid, succinimidyl ester] Amino-reactive biotinylating reagent for peptides and proteins; Biotin-X, SSE [6-((Biotinoyl)amino) hexanoic acid, sulfosuccinimidyl ester, sodium salt] Biotin-XX, SE [6-((6-((Biotinoyl)amino) hexanoyl)amino) hexanoic acid, succinimidyl ester] Biotinylating reagent for peptides and proteins FMOC biocytin [FMOC-Lys(Biotin)-OH] Building block for preparing biotinylated peptides Qty 100 mg
60649 1g 10 ml 100 mg 60651 100 mg
25 mg
60650
25 mg
25 mg
21113
21115
100 mg
60652
25 mg
60640
25 mg
21117 100 mg
100 mg
21117
Thiol-Reactive Biotins
Cat. No. 60642 Product Biocytin C2 maleimide Excellent thiol-reactive biotin with a moderate spacer for better avidin-binding Biotin C2 maleimide Excellent thiol-reactive biotin with a moderate spacer for better avidin-binding N-(Biotinoyl)-N-(iodoacetyl)ethylenediamine Thiol-reactive biotinylating reagent for proteins and other biomolecules. Qty 10 mg
60643
25 mg
60644
25 mg
Amino-containing Biotins for Modifying Carbohydrates, Carbonyl, Carboxyl Groups

Cat. No. 60645 Product ARP [N-(aminooxyacetyl)-N-(D-biotinoyl) hydrazine, trifluoroacetic acid salt] Biotinylating reagent for carbohydrates and nucleic acids Biocytin [e-Biotinoyl-L-lysine] More polar than biotin; used for cell tracing Qty 10 mg
60638
100 mg
114
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Biotin, Avidin, Streptavidin

Streptavidin and Its Conjugates
Cat. No. 60659 Product Streptavidin Highly purified and tested for biotin-binding Streptavidin, alkaline phosphatase conjugated *2 mg/mL* used in ELISA assays Streptavidin, allophycocyanin conjugated *2 mg/mL* abs: 652 nm; em: 662 nm used in immunofluorescence assays Streptavidin, B-phycoerythrin conjugated *2 mg/mL* abs: 545 nm; em: 575 nm used in immunofluorescence assays Streptavidin, crosslinked allophycocyanin conjugated *2 mg/mL* abs: 616 nm; em: 647 nm used in immunofluorescence assays Streptavidin, 5-FAM conjugated abs: 492 nm; em: 519 nm used in immunofluorescence assays Streptavidin, HiLyte Fluor 488 conjugated abs: 495 nm; em: 524 nm Widely used in immunofluorescence assays; stronger signal and better stability than FITC-streptavidin Streptavidin, HiLyte Fluor 555 conjugated abs: 555 nm; em: 565 nm Widely used in immunofluorescence assays; comparable spectral properties to those of Cy3 or Cy3B Streptavidin, HiLyte Fluor 647 conjugated abs: 650 nm; em: 668 nm Widely used in immunofluorescence assays; comparable spectral properties to those of Cy5 Streptavidin, HRP conjugated abs: N/A nm; em: N/A nm Widely used in ELISA assays Streptavidin, R-phycoerythrin conjugated *2 mg/mL* abs: 565 nm; em: 575 nm Widely used in immunofluorescence assays Streptavidin, 5-TAMRA conjugated abs: 541 nm; em: 568 nm used in immunofluorescence assays Streptavidin, HiLyte Fluor TR conjugated abs: 591 nm; em: 622 nm used in immunofluorescence assays; comparable spectral properties to those of sulforhodamine 101 sulfonyl chloride (also called Texas Red ) with stronger fluorescence intensity Qty 5 mg
ANTIBODIES
Biotin-Avidin ..
Biotin Quantitation Reagents and Fluorescent Biotins

Cat. No. 71161 Product HABA Biotin Quantitation Kit *Colorimetric* abs: 348 nm; em: N/A nm Colorimetric determination of biotin Qty 1 kit
60660
0.25 ml
60661
0.10 ml
Cat. No. 60654
Product Biotin-4-fluorescein abs: 494 nm; em: 523 nm Demonstrate better binding and stronger fluorescence than biotin fluorescein Biotin-X NTA [Biotin-X nitrilotriacetic acid, tripotassium salt] Excellent reagent for detecting polyhistidine-containing biomolecules Fluorescein biotin [5-((N-(5-(N-(6 (Biotinoyl)amino)hexanoyl)amino)pentyl) thioureidyl) fluorescein] abs: 494 nm; em: 518 nm Green fluorescent biotin used for studying avidin binding HABA [2-4(-hydroxyazobenzene) benzoic acid] *UltraPure Grade* abs: 348 nm; em: N/A nm Colorimetric indicator for biotin TMR Biocytin [5-(and-6)Tetramethylrhodamine biocytin] abs: 554 nm; em: 581 nm Orange fluorescent biotin used for studying avidin binding
Qty 10 mg
60662
0.25 ml 60655 0.10 ml 60656
5 mg
60663
5 mg
60664
1 mg
60665
1 mg
60657
1g
60658 1 mg
5 mg
60666
60667
1 mg
Antibody Reagents
Cat. No. 29704 Product Antibody Diluent (Ready to Use) AnaPrep IHC Histain Kit-AEC AnaPrep AEC Substrate System AnaPrep IHC Histain Kit-DAB Maleimidyl KLH Maleimidyl BSA Qty 125 mL 15 mL 125 mL 15 mL 5 mg 5 mg
60668
1 mg
29702 29703
60669
0.25 ml
29700 29551
60670
1 mg
29552
60671
1 mg
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115
Prion Proteins and cDNAs

HumanPrion Proteins
Cat. No. PR-902 Product PrPc (full length, residues 23-231) His (Prion Protein, cellular form) Human, Recombinant, E. coli Native PrPc (full length, residues 23-231) His (Prion Protein, cellular form) Human, Recombinant, E. coli PrPc (globular domain, residues 90-231) His (Prion Protein, cellular form)Human, Recombinant, E. coli Native PrPc (globular domain, residues 90-231) His (Prion Protein, cellular form)Human, Recombinant, E. coli Qty 50 g
Prion Proteins
Mouse Prion Proteins

Cat. No. PR-904 Product PrPc (full length, residues 23-230)His (Prion Protein, cellular form) Mouse, Recombinant, E. coli Native PrPc (full length, residues 23-230)His (Prion Protein, cellular form) Mouse, Recombinant, E. coli PrPc (globular domain, residues 90-230)His (Prion Protein, cellular form) Mouse, Recombinant, E. coli Native PrPc (globular domain, residues 90-230)His (Prion Protein, cellular form) Mouse, Recombinant, E. coli Qty 50 g
PR-908
5 g
PR-910
5 g
PR-905
50 g PR-907 5 g PR-913 50 g
PR-911
5 g
Bovine Prion Proteins

Cat. No. PR-370 Product PrP (wild type) (Prion Protein, cellular form) Bovine, Recombinant, E. coli PrPc (T194A)His (Prion Protein, cellular form) Bovine, Recombinant, E. coli PrPc (F209S)His (Prion Protein, cellular form) Bovine, Recombinant, E. coli PrP (Q228R) (Prion Protein, cellular form) Bovine, Recombinant, E. coli PrPc (A144V)His (Prion Protein, cellular form) Bovine, Recombinant, E. coli
c His c His
Qty 50 g
Prion associated Proteins

N-terminal GST tagged Src SH3 domain. Src SH3 domain is the SH3 domain of Src tyrosine kinase. SH3 domains are known to bind proline rich peptides. Human lymphocyte specific kinase (Lck) is involved in T-cell- and IL2receptor signaling. Lck SH3 domain is the SH3 domain of Lck tyrosine kinase. SH3 domains are known to bind proline rich peptides. GABARAP is essential for correct delivery of newly synthesized or recycled GABA type A receptor molecules to the postsynaptic membrane neurons. Cat. No. Product SrcGST SH3 domain(Sarcoma inducing tyrosine kinase, SH3 domain) Human LckGST SH3 domain(Lymphocytespecific Kinase, SH3 Domain)Human, GABARAPGST(GABA Type A Associated Protein)Human, Qty 50 g
PR-3v71
50 g
PR-372
50 g
PR-373
50 g
PR-374
50 g PR-338
Ovine (Sheep) Prion Proteins

Cat. No. PR-376 Product PrPcHis Genotyp: ARR (Codons 136,154,171), scrapie resistant ovine (sheep), Recombinant, E. coli PrPcHis Genotyp: VRQ (Codons 136,154,171), scrapie sensitive ovine (sheep), Recombinant, E. coli Qty 50 g
PR-339 PR-1000
50 g 50 g
PR-377
50 g
BovPrPC cDNA
Cat. No. PRD-370 Product bovPrPc cDNA, wild type (cDNA from bovine Prion Protein, cellular form) bovPrPc cDNA [T194A] (cDNA from bovine Prion Protein, cellular form) bovPrPc cDNA [F209S] (cDNA from bovine Prion Protein, cellular form) bovPrPc cDNA [Q228R] (cDNA from bovine Prion Protein, cellular form) bovPrPc cDNA [A144V] (cDNA from bovine Prion Protein, cellular form) Qty
Hamster Prion Proteins

Cat. No. PR-903 Product PrPc (full length, residues 23-231)His (Prion Protein, cellular form) Hamster, Recombinant, E. coli Native PrP (full length, residues 23-231)His (Prion Protein, cellular form) Hamster, Recombinant, E. coli PrPc (globular domain, residues 90-231) His (Prion Protein, cellular form) Hamster, Recombinant, E. coli Native PrPc (globular domain, residues 90-231)His (Prion Protein, cellular form) Hamster, Recombinant, E. coli
c
Qty 50 g PRD-371
10 g
10 g
PR-909
50 g
PRD-372
10 g
PR-906
50 g
PRD-373
10 g
PR-912
50 g
PRD-374
10 g
ATG-start lodon and different restriction sites for subcloning on request.
116
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Viral Proteins- CMV, EBV, Herpes, Hepatitis A, B, C

Cytomegalovirus (CMV)
Cat. No. PR-1247 Product CMV-gB (residues 11-76) (Cytomegalo Virus Glycoprotein B) CMV pp28 (residues 130-160) (Cytomegalo Virus Phosphoprotein 28) CMV pp38 (residues 117-373) (Cytomegalo Virus Phosphoprotein 38) CMV pp52 (residues 202-434) (Cxtomegalo Virus Phosphoprotein 52) CMV pp65 (residues 297-510) (Cytomegalo Virus Phosphoprotein 65) CMV pp150 (residues 1011-1048) (Cytomegalo Virus Phosphoprotein 150) Qty 100 g
Viral Proteins
Hepatitis A (Contd.)
Cat. No. PR-1114 Product HAV-VP1-P1A/Mosaic (residues 669-782, 722-830) (Hepatitis A Virus Coat Protein VP1-Core Protein P2A Mosaic) HAV-VP1-P2A (residues 669-782) (Hepatitis A Virus Coat Protein VP1- Core Protein P2A) HAV-P2C-P3A/1 (residues 1392-1521) (Hepatitis A Virus Core Protein P2C-P3A fragment 13-15) HAV-P2C-P3A/2 (residues 1492-1606) (Hepatitis A Virus Core Protein P2C-P3A fragment 14-16) HAV-P2C-P3A/Mosaic (residues 1392-1521, 1492-1606) (Hepatitis A Virus Core Proteins P2C-P3A Mosaic) HAV-P2C (residues 1211-1234) (Hepatitis A Virus Core Protein P2C) HAV-P3C (residues 1643-1743) (Hepatitis A Virus Core Protein P3C) Qty 100 g
PR-1248
100 g PR-1113 100 g PR-1117 100 g PR-1118
100 g
PR-1249
100 g
PR-1250
100 g
PR-1251
100 g PR-1116
PR-1252
100 g
100 g
Epstein-Barr Virus (EBV)

Cat. No. PR-1223 Product EBV-EBNA1 Mosaic (residues 1-90, 408-498) (Epstein-Barr Virus Nuclear Protein 1) EBV-EA (residues 306-390) (Epstein-Barr Virus Early Antigen) EBV p18 (residues 1-119) (Epstein-Barr Virus Capsid Antigen) EBV p23 (residues 1-162) (Epstein-Barr Virus Capsid Antigen) Qty 100 g
PR-1115 PR-1121
100 g 100 g
Hepatitis B
Cat. No. PR-1122 Product HBV-Core (residues 1-186) (Hepatitis B Virus Core Protein) HBV-Core 16 kDa (residues 1-144) (Hepatitis B Virus Core Protein) HBV-Core 18 kDa (residues 1-183) (Hepatitis B Virus Core Protein) HBV-HBe (Hepatitis B Virus e Antigen, HBVeAg) HBVsAg-ayw (Hepatitis B Virus Surface Antigen, ayw subtype) HBVsAg-ayw (Hepatitis B Virus Surface Antigen, ayw subtype) HBVsAg-adr (Hepatitis B Virus Surface Antigen, adr subtype) HBVsAg-adw (Hepatitis B Virus Surface Antigen, adw subtype) Qty 100 g 100 g 100 g 100 g 100 g
PR-1224 PR-1225 PR-1226
100 g 100 g
PR-1124 100 g PR-1123 PR-1125
Herpes
Cat. No. PR-1108 PR-1109 PR-1110 PR-1111 Product HSV-1 gD (residues 266-394) (Herpes Simplex Virus-1 glycoprotein D) HSV-1 gG (residues 84-175) (Herpes Simplex Virus-1 glycoprotein G) HSV-2 gD (residues 266-384) (Herpes Simplex Virus-2 glycoprotein D) HSV-2 gG (residues 525-578) (Herpes Simplex Virus-2 glycoprotein G) Qty 100 g 100 g 100 g
PR-1126
PR-1129
100 g
PR-1127
100 g
PR-1128 100 g
100 g
Hepatitis C Hepatitis A
Cat. No. PR-1112 PR-1120 Product HAV-VP1 (residues 524-633) (Hepatitis A Virus Coat Protein VP1) HAV-VP2-VP4 (residues 55-164) (Hepatitis A Virus Capsid Proteins VP4-VP2) HAV-VP3 (residues 304-415) (Hepatitis A Virus VP3 Capsid Protein) Qty 100 g 100 g Cat. No. PR-1131 PR-1130 Product HCV-N-G1 (residues 1-102) (Hepatitis C Virus Nucleocapsid Genotype 1) HCV-N-G1a (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 1a) HCV-N-G1b (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 1b) HCV-N-G2a (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 2a) Qty 100 g 100 g
PR-1132 PR-1133
100 g 100 g
PR-1119
100 g
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117
Viral Proteins- Hepatitis C

Hepatitis C (Contd.)
Cat. No. PR-1134 Product HCV-N-G2b (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 2b) HCV-N-G3a (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 3a) HCV-N-G3b (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 3b) HCV-N-G4 (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 4) HCV-N-G5 (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 5) HCV-N-G6a (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 6a) HCV-N-G3/10 (residues 2-119) (Hepatitis C Virus Nucleocapsid Genotype 3/10) HCV-N (residues 105-302) (Hepatitis C Virus Nucleocapsid) HCV-N24 (Hepatitis C Virus Nucleocapsid) C-HCV (Combined Hepatitis C Virus, NS3/NS4/NS5) HCV-NC 22 kDa (residues 2-192) (Hepatitis C Virus Nucleocapsid) HCV-NC 22 kDa (residues 2-192), Biotin conjugated (Hepatitis C Virus Nucleocapsid) HCV-NC 22 kDa (residues 2-192), Fluorescein conjugated (Hepatitis C Virus Nucleocapsid) HCV-NC 22 kDa (residues 2-192), Rhodamine conjugated (Hepatitis C Virus Nucleocapsid) HCV-NS3His (Hepatitis C Virus Non-Structural protein) HCV-NS3-S1a (residues 1192-1459) (Hepatitis C Virus Non-Structural protein, Subtype 1a) HCV-NS3-S1a (residues 1359-1456) (Hepatitis C Virus Non-Structural protein, Subtype 1a) HCV-NS3-S1b (residues 1192-1459) (HCV Non-Structural protein, Subtype 1b) HCV-NS3-S1b (residues 1359-1456) (HCV Non-Structural protein, Subtype 1b) HCV-NS3-S1c (residues 1192-1459) (HCV Non-Structural protein, Subtype 1c) HCV-NS3-S2b (residues 1192-1459) (HCV Non-Structural protein, Subtype 2b) Qty 100 g
Viral Proteins
Cat. No. PR-1155 Product HCV-NS3-S2b (residues 1359-1456) (HCV Non-Structural protein, Subtype 2b) CV-NS3-S2c (residues 1192-1459) (HCV Non-Structural protein, Subtype 2c) HCV-NS3-S3 (residues 1359-1456) (HCV Non-Structural protein, Subtype 3) HCV-NS3-S4 (residues 1359-1456) (HCV Non-Structural protein, Subtype 4) HCV-NS3-S5 (residues 1359-1456) (HCV Non-Structural protein, Subtype 5) HCV-NS3-S5a (residues 1192-1459) (HCV Non-Structural protein, Subtype 5a) HCV-NS3-S6 (residues 1359-1456) (HCV Non-Structural protein, Subtype 6) HCV-NS3-S6a (residues 1192-1459) (HCV Non-Structural protein, Subtype 6a) HCV-NS3-22 kDa (residues 1400-1643) (HCV Non-Structural protein) HCV-NS3 22 kDa (residues 1400-1643), Biotin conjugated (HCV Non-Structural protein) HCV-NS4, Mosaic (HCV Non-Structural protein) HCV-NS4-G1, Mosaic (residues 16911710, 1712-1733, 1921-1940) (HCV Non-Structural protein, Genotype 1) HCV-NS4-G2, Mosaic (residues 1691-1710, 1712-1733, 1921-1940) (HCV Non-Structural protein, Genotype 2) HCV-NS4-G3, Mosaic (residues 16911710, 1712-1733, 1921-1940) (HCV Non-Structural protein, Genotype 3) HCV-NS4-G5, Mosaic (residues 1691-1710, 1712-1733, 1921-1940) (HCV Non-Structural protein, Genotype 5) HCV-NS4 (residues 1916-1947) (HCV Non-Structural protein) HCV-NS4 19kDa (residues 1658-1863) (HCV Non-Structural protein) HCV-NS4 19kDa (residues 1658-1863), Biotin conjugated (HCV Non-Structural protein) HCV-NS4 19kDa (residues 1658-1863), Horseradish peroxidase conjugated (HCV Non-Structural protein) HCV-NS4 19kDa (residues 1658-1863), Fluorescein conjugated (HCV Non-Structural protein) Qty 100 g
PR-1135
100 g
PR-1149
100 g
PR-1136
100 g PR-1156 100 g PR-1157
100 g
PR-1137
100 g
PR-1138
100 g PR-1158
PR-1139
100 g
100 g
PR-1140
100 g
PR-1152
100 g
PR-1141
100 g
PR-1159
100 g
PR-1142 PR-1143 PR-1144 PR-1144-B HRP PR-1144-F
100 g PR-1151 100 g 100 g 100 g PR-1160 PR-1160-B/ HRP PR-1161 PR-1162 100 g PR-1163 100 g 100 g PR-1164
100 g
100 g 100 g
100 g
100 g 100 g
PR-1144-R
100 g
PR-1145 PR-1146
100 g
PR-1153
100 g
PR-1165
100 g
PR-1147
100 g
PR-1166 PR-1167
100 g 100 g 100 g
PR-1154
100 g PR-1167-B 100 g PR-1167HRP 100 g PR-1167-F
PR-1148
100 g
PR-1150
100 g
118
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Viral Proteins- Hepatitis C, HIV-1

Cat. No. PR-1167-R Product HCV-NS4 19kDa (residues 1658-1863), Rhodamine conjugated (HCV Non-Structural protein) HCV-NS4 19kDa (residues 1658-1863), Rhodamine conjugated (HCV Non-Structural protein) HCV-NS5 (residues 2322-2423) (HCV Non-Structural protein) HCV-NS5-G1 (HCV Non-Structural protein, Genotype 1) HCV-NS5-G1a (residues 2212-2313) (HCV Non-Structural protein, Genotype 1a) HCV-NS5-G1b (residues 2212-2313) (HCV Non-Structural protein, Genotype 1b) HCV-NS5-G2 (HCV Non-Structural protein, Genotype 2) HCV-NS5-G2a (residues 2212-2313) (HCV Non-Structural protein, Genotype 2a) HCV-NS5-G2b (residues 2212-2313) (HCV Non-Structural protein, Genotype 2b) HCV-NS5-G3 (HCV Non-Structural protein, Genotype 3) HCV-NS5-G3a (residues 2212-2313) (HCV Non-Structural protein, Genotype 3a) HCV-NS5-G3b (residues 2212-2313) (HCV Non-Structural protein, Genotype 3b) HCV-NS5-G4 (HCV Non-Structural protein, Genotype 4) HCV-NS5-G5 (HCV Non-Structural protein, Genotype 5) HCV-NS5-G6 (HCV Non-Structural protein, Genotype 6) HCV-NS5-G6a (residues 2212-2313) (HCV Non-Structural protein, Genotype 6a) Qty 100 g
Viral Proteins
Hepatitis E (Contd.)
Cat. No. PR-1186 PR-1188 100 g PR-1185 100 g Product HEV-ORF2 (residues 403-461) (Hepatitis E Virus ORF Frame protein) HEV-ORF2 (residues 452-617) (Hepatitis E Virus ORF protein) HEV-ORF2 (residues 633-659) (Hepatitis E Virus ORF protein) Qty 100 g 100 g 100 g
PR-1167-R
PR-1168 PR-1169 PR-1170 PR-1171 PR-1172 PR-1173 PR-1174 PR-1175 PR-1176
HIV-1
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g Cat. No. PR-1200 PR-1201 PR-1201-B PR-1201HRP PR-1202 PR-1203 PR-1207 PR-1213 PR-1204 PR-1204-B 100 g 100 g 100 g 100 g 100 g PR-1204HRP PR-1205 PR-1205HRP PR-1206 PR-1217 PR-1215 PR-1209 Product HIV-1 Envelope (HIV 1 Antigen) HIV-1 p24 (HIV 1 Antigen) HIV-1 p24, biotin conjugated (HIV 1 Antigen) HIV-1 p24, horse radish peroxidase labeled (HIV 1 Antigen) HIV-1 p17, p24, gp120, gp41 (HIV 1 Antigens) HIV-1 p17, p24, gp120 (HIV 1 Antigens) HIV-1 p17, p24 (HIV 1 Antigens) HIV-1 p24 (residues 77-436) (HIV 1 Antigen) HIV-1 gp41 (HIV 1 Antigen) HIV-1 gp41, biotin conjugated (HIV 1 Antigen) HIV-1 gp41, horse radish peroxidase conjugated (HIV 1 Antigen) HIV-1 gp41 Long (HIV 1 Antigen) HIV-1 gp41 Long, horse radish peroxidase conjugated (HIV 1 Antigen) HIV-1 gp41 (residues 466-753) (HIV 1 Antigen) HIV-1 p55 (HIV 1 Antigen) HIV-1 gp160 (HIV 1 Antigen) HIV-type O Envelope (HIV Type O Antigen) HIV-1 Integrase (HIV 1 Integrase) HIV-1 TAT (HIV 1 Trans-Acting Transcription factor) HIV-1 Nef protein (full length, 1-210)His ((HIV -1, Negative Factor) HIV-1 Nef protein (full length, 2-210)His, myristoylated (HIV-1, Negative Factor) HIV-1 Nef (residues 3-190) (HIV 1 Antigen) HIV-1 RT (HIV 1 Reverse Transcriptase) HIV-1 RT, p51 subunit (HIV-1 Reverse Transcriptase) HIV-1 RT, p66 subunit (HIV 1 Reverse Transcriptase) HIV-1 Vpu-43GST (HIV 1, Virus Protein U, residues 39-81 Qty 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g
PR-1177 PR-1178 PR-1179 PR-1180 PR-1181
100 g 100 g 10 g 10 g 100 g 100 g 10 g 50 g 50 g
Hepatitis D
Cat. No. PR-1182 Product HDV (residues 1-108, 151-209) (Hepatitis Delta Virus) Qty 100 g
PR-1208 PR-1216 PR-381 PR-382
Hepatitis E
Cat. No. PR-1183 Product HEV-ORF2/ORF3/1 Mosaic (Hepatitis E Virus ORF proteins, 4 immunodominant regions) HEV-ORF2/ORF3/2 Mosaic (Hepatitis E Virus ORF proteins, 12 immunodominant regions) HEV-ORF2 (residues 92-123) (Hepatitis E Virus ORF protein) Qty
PR-1214 PR-351
100 g 10 g 10 g 10 g 100 g
100 g PR-353
PR-1184
100 g
PR-354 PR-380
PR-1187
100 g
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119
HIV-2, Rubella, VZV, TBEV, Taxoplasma, Treponema

HIV-2
Cat. No. PR-1218 PR-1210 PR-1211 PR-1212 PR-352 Product HIV-2 Envelope (HIV 2 Antigen) 100 g HIV-2 gp32 (HIV 2 Antigen) HIV-2 gp36 (HIV 2 Antigen) HIV-2 gp36 (residues 390-702) (HIV 2 Antigen) HIV-2 RT (HIV 2 Reverse Transcriptase) 100 g 100 g 100 g 10 g Qty
Viral Proteins
Varizella-Zoster-Virus (VZV)
Cat. No. PR-1253 PR-1254 PR-1255 Product VZV-gE (aa 48-135) (Varicella-zoster Virus Glycoprotein E) VZV ORF9 (residues 6-28, 76-100) (Varicella-zoster Virus Protein) VZV ORF26 (residues 9-33, 184-208) (Varicella-zoster Virus Protein) Qty 100 g 100 g 100 g
Tick-Borne Encephalitis Virus (TBEV)

Cat. No. PR-1227 Product TBEV gE (residues 95-229) (Tick-borne Encephalitis Virus Glycoprotein E) Qty 100 g
Human T-cell Lymphotropic Virus (HTLV)

Cat. No. PR-1219 PR-1220 PR-1221 PR-1222 Product HTLV-1 Envelope HTLV-1 p24 Core HTLV-1 Mosaic (residues 374-400, 190-207) HTLV-1+2 (fusion protein) Qty 100 g 100 g
Toxoplasma
Cat. No. Product TG MIC3 (residues 234-306) (Toxoplasma Gondii Microneme Protein 3) TG GRA1/p24 (residues 57-149) (Toxoplasma Gondii Dense Granule Protein 1) TG GRA7/p29 (residues 24-100) (Toxoplasma Gondii Dense Granule Protein 7) TG SAG1/p30 (residues 45-198) (Toxoplasma Gondii Surface Antigen 30) Qty 100 g 100 g 100 g 100 g
100 g PR-1243 100 g PR-1244
Rubella Virus (RV)

Cat. No. PR-1228 Product RV gE1 Mosaic (residues 157-176, 374-390, 213-239) (Rubella Virus Glycoprotein E1) RV gE2 (residues 31-105) (Rubella Virus Envelope Glycoprotein E2) RV-C (residues 1-123) (Rubella Virus Capsid Protein) Qty 100 g
PR-1245 PR-1246
Treponema
100 g 100 g Cat. No. PR-1239 PR-1238 Product TRP p15 (T. Pallidum Immunogen) TRP p17 (T. Pallidum Immunogen) TRP p41 (T.Pallidum Immunogen) TRP p47 (T. Pallidum Immunogen) TmpA (T.Pallidum Protein A) Qty 100 g 100 g 100 g 100 g 100 g
PR-1229 PR-1230
SARS
Cat. No. PR-1100 PR-1101 PR-1103 PR-1102 PR-1104 PR-1105 Product SARS-ACE (residues 1-76) (SARS-Associated Coronavirus Envelope) SARS-ACM (residues 182-216) (SARS-Associated Coronavirus Matrix) SARS-ACN/2 (residues 1-49) (SARS-Associated Coronavirus Nucleocapsid) SARS-ACN/1 (residues 340-390) (SARS-Associated Coronavirus Nucleocapsid) SARS-ACN/3 (residues 1-49, 192-220) (SARS-Associated Coronavirus Nucleocapsid) SARS-ACSMS(C) (residues 1051-1076, 1121-1154, 1162-1190) (SARS-Associated Coronavirus Spike Mosaic S(C)) SARS-ACSMS(M) (residues 408-470, 540-573) (SARS-Associated Coronavirus Spike Mosaic S(M)) Qty
PR-1240 PR-1241 100 g PR-1242 100 g 100 g 100 g 100 g 100 g
PR-1106
100 g
120
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Transcription Factors - General, Activators

General Transcription Factors
Cat. No. PR-703 PR-781 PR-701 PR-786 PR-785 PR-700 PR-784 PR-731 PR-702 PR-787 PR-712 PR-706 PR-704 PR-788 PR-705 PR-709 Product TBP (TATA box Binding Protein) TBPGST (TATA box Binding Protein) TFIIA-p12 (Transcription Factor IIA, p12 subunit) TFIIAGST (p12) (Transcription Factor IIA, p12 subunit) TFIIAGST (p19) (Transcription Factor IIA, -subunit) TFIIA-p55 (Transcription Factor IIA, p55 subunit) TFIIAGST (p55) (Transcription Factor IIA, p55 subunit) TFIIA (Transription Factor IIA, reconstituted) TFIIB (Transcription Factor IIB) TFIIBGST (Transcription Factor IIB) TFIID (Transcription Factor IID, native complex) TFIIE (Transcription Factor IIE, - + -subunits) TFIIE, p56 (Transcription Factor IIE, -subunit) TFIIEGST, p56 (Transcription Factor IIE, -subunit) TFIIE, p34 (Transcription Factor IIE, -subunit) TFIIF (Transcription Factor IIF, Rap30 + Rap74 subunits) TFIIF, RAP74 (Transcription Factor IIF, Rap74 subunit) RAP74 GST (Transcription Factor IIF, Rap74 subunit) TFIIF, RAP30 (Transcription Factor IIF, Rap30 subunit) RAP30 GST (Transcription Factor IIF, Rap30 subunit) TFIIH (Transcription Factor IIH, native complex) TFIIH-p62 (Transcription Factor IIH, p62 subunit) RNA pol II (RNA Polymerase II, native complex) RNA pol II-CTD (RNA Polymerase II, Carboxy-terminal Domain) RNA pol II-CTDGST (RNA Polymerase II, Carboxyterminal Domain) RNA pol II-hRPB5 (RNA Polymerase II, p33 subunit) RNA pol II-hRPB5GST (RNA Polymerase II, p33 subunit) RNA pol II-hRPB6GST (RNA Polymerase II, p15.6 subunit) Qty 10 g 10 g 10 g 10 g 10 g 10 g
Transcription Factors
General Transcription Factors (Contd.)

Cat. No. PR-716 PR-793 PR-794 PR-795 Product RNA pol II-hRPB9 (RNA Polymerase II, p14.5 subunit) RNA pol II-hRPB9 (RNA Polymerase II, p14.5 subunit) RNA pol II-hRPB10GST (RNA Polymerase II, RPB10 subunit) RNA pol II-hRPB12GST (RNA Polymerase II, RPB12 subunit)
GST
Qty 10 g 10 g 10 g 10 g
Transcription Factors Activators

Cat. No. Product Bcl-10 (B-cell lymphoma 10) BRCA1 (Breast/Ovary Cancer Gene 1 Product, Tumor Suppressor Protein and Transcription Factor) CTF1 (CCAAT-Box-Binding Transcription Factor 1) CTF1GST-Tag (CCAAT-Box-Binding Transcription Factor 1, activation domain, residues 399-499) c-fos (Proto-oncogene, Cellular oncogene fos, G0/G1 switch regulatory protein 7) c-jun (Proto-oncogene, Transcription Factor Activator Protein-1) c-myc (Proto-oncogene) E2F-1 (RBAP-1, Transcription Factor and Rb-mediator) FGF-1 (Acidic Fibroblast Growth Factor) FGF-2 (Basic Fibroblast Growth Factor) GAL4-AH [Positive regulator of galactose inducible genes, GAL4(1-147) fused to an a-Helix] GAL4-VP16 [Positive regulator of galactose inducible genes, GAL4(1-147) fused to VP16(411-490) herpes simplex virus virion transactivating protein] VP16 GST [VP16(411-490), herpes simplex virus virion transactivating protein]i GAL4-E1A [Positive regulator of galactose inducible genes, GAL4(1-147) fused to E1A(121-223), Ubiquitin-like 1 activating enzyme E1A (SUMO-1 activating enzyme] Qty 6 g 2 g
10 g 10 g 10 g 10 g 2 g
PR-769 PR-763
PR-734 10 g PR-798 10 g 10 g 10 g PR-772 10 g PR-773 10 g 10 g 10 g 10 g PR-810 2 g PR-717 10 g 2 g 10 g PR-718 PR-730 PR-771
5 g 0 g
5 g
5 g
5 g 5 g
PR-707 PR-789 PR-708 PR-790 PR-710 PR-711 PR-713 PR-714
PR-809
5 g 5 g 10 g
10 g
PR-796
10 g
PR-797
10 g
PR-715 PR-791 PR-792
10 g 10 g 10 g
PR-719
4 g
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Transcription Factors - Activators, Coactivators, Repressors

Transcription Factors Activators (Contd.)
Cat. No. PR-720 Product GAL4-Sp1Q [Positive regulator of galactose inducible genes, GAL4(94) fused to transcription factor Sp1(266-516)] Sp1 (HeLa) (GC-box Binding Protein, Natural fraction) Sp1 (Sf9) (GC-box Binding Protein ) Sp1GST (GC-box Binding Protein, GST-tagged) p53 (Tumor Suppressor Protein and Transcription Factor, wild type) p53 (C-terminal deletion 1-363) (Tumor Suppressor Protein and Transcription Factor, residues 1-363, C-terminal deletion) p53 (C-terminal deletion 1-342) (Tumor Suppressor Protein and Transcription Factor, residues 1-342, C-terminal deletion) p53GST (wild type) (Tumor Suppressor Protein and Transcription Factor, residues 1-81) p53GST (mt, L22Q and W23S) (Tumor Suppressor Protein and Transcription Factor, residues 1-81) pRB (Retinoblastoma Protein, Tumor Suppressor Protein, Transcription Factor and Proliferation Factor) RAD51 (RecA-like protein, recombination repair protein) WT-1 (+ KTS) (The Wilms Tumor Suppressor and Transcription/Pre-mRNA Splicing Factor) WT-1 (- KTS) (The Wilms Tumor Suppressor and Transcription/Pre-mRNA Splicing Factor) Qty 4 g
Transcription Factors
Transcription Factors Coactivators (Contd.)

Cat. No. PR-770 PR-725 2 g PR-726 5 g PR-727 10 g 5 g PR-735 PR-736 5 g PR-737 5 g PR-738 5 g Product p300 (C1135-2414) (p300 C-terminus HAT Domain) PC4 (Positive Cofactor 4, Transcriptional Coactivator wild type) PC4-mt (F77P) (Positive Cofactor 4, F77P mutant, Transcriptional Coactivator) PC4-mt (serine mutations) (Positive Cofactor 4, serine mutations, Transcriptional Coactivator) Topo I (Human DNA Topoisomerase I, wild type) Topo I CTD [Human DNA Topoisomerase I, C-terminal Domain (CTD)] Topo I Core (Human DNA Topoisomerase I, Core Domain) Topo I NTD [Human DNA Topoisomerase I, N-terminal Domain (NTD)] Topo I (Y723F) (Human DNA Topoisomerase I) Qty 4 g 10 g
PR-732 PR-733 PR-799 PR-759
10 g
10 g
2 g 2 g
PR-760
2 g 2 g
PR-761
PR-782
PR-739
2 g
PR-783
10 g
Transcription Factors Repressors

Cat. No. Product Dr1 (Down-regulator of transcription 1, NC2, 19 kDa) Dr1 GST (Down-regulator of transcription 1, NC2, 19 kDa) HMG1 (High Mobility Group 1) HMG1 (Native, High Mobility Group 1) Bovine, calf thymus Qty 10 g
PR-765
5 g PR-723
PR-808
10 g
PR-800
10 g
PR-766
5 g
PR-724 PR-757
10 g 10 g
PR-767
5 g
Transcription Factors Coactivators

Cat. No. PR-740 PR-741 PR-728 PR-729 Product BRG1 (Brahma-related Gene 1 Protein, wild type) BRG1-mt (Brahma-related Gene 1 Protein, K798R) p52 (Transcriptional Coactivator) p75 (Transcriptional Coactivator and Lens Epithelial Cell-Derived Growth Factor) p75-CTR [C-terminal region of p75 (LEDGF)] p300 (Tumor Suppressor Protein and Transcription Factor) Qty 5 g 5 g 10 g
Transcription Factors - Pre-mRNA Splicing Factors

Cat. No. PR-774 Product ASF/SF2 (SFRS1 or SRp30a) (Splicing Factor, Arginine/Serine-rich 1, Pre-mRNA Splicing Factor) Human, ASF/SF2 (SFRS1 or SRp30a) (Splicing Factor, Arginine/Serine-rich 1, Pre-mRNA Splicing Factor) Human, SC35 (SFRS2 or SRp30b) (Splicing Component, 35 kDa, Pre-mRNA Splicing Factor) Human, WT-1 (+ KTS) (The Wilms Tumor Suppressor and Transcription/Pre-mRNA Splicing Factor) WT-1 (- KTS) (The Wilms Tumor Suppressor and Transcription/Pre-mRNA Splicing Factor) Qty 10 g
PR-775
5 g
PR-776 10 g PR-766 10 g 2 g PR-767
5 g
5 g
PR-758 PR-762
5 g
122
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Nuclear Receptors/Hematopoetins
Nuclear Receptors
Cat. No. PR-756 PR-743 PR-804 PR-750 PR-744 PR-805 PR-813 Product ER (Estrogen Receptor, -isoform) FXR (Farnesoid-X-activated Receptor) FXR
GST
Hematopoetins/Nuclear Receptor
Nuclear Receptors (Contd.)

Qty 4 g 10 g 10 g 5 g 10 g 10 g 10 g PR-742 10 g PR-801 10 g PR-752 10 g PR-802 10 g 10 g PR-751 PR-803 PR-722 10 g PR-826 PR-721 PR-821 Cat. No. PR-812 Product RAR- (Retinoic Acid Receptor, -isoform)
GST
Qty 10 g 10 g
(Farnesoid-X-activated Receptor)
GR (Glucocorticoid Receptor) LXR- (Liver-X Receptor, -isoform) LXR-

GST
RAR- -LBD (residues 150-417) (Retinoic Acid Receptor, -isoform, ligand binding domain) RAR- -LBDGST (residues 150-417) RXR- (Retinoid X Receptor, -isoform) RXR--LBD (residues 200-462) (Retinoid X Receptor, -isoform, Ligand Binding Domain) RXR--LBDGST (residues 200-462) (Retinoid X Receptor, -isoform, Ligand Binding Domain) RXR- (Retinoid X Receptor, -isoform) RXR- GST TR- 1 (Thyroid Hormone Receptor, 1-isoform) TR- 1GST TR- 1 (Thyroid Hormone Receptor, 1-isoform)
10 g 10 g 10 g
(Liver-X Receptor, -isoform)
LXR- -N182GST (Liver-X Receptor, -isoform, N-terminal 182 region) LXR- -LBD (Liver-X receptor, -isoform, ligand binding domain) LXR- -LBD GST (Liver-X Receptor, a-isoform, ligand binding domain) LXR- (Liver-X Receptor, -isoform) LXR- (Liver-X Receptor, -isoform)
GST
PR-746
10 g
PR-807
5 g 10 g 5 g 10 g 10 g
PR-745 PR-806 PR-747
PPAR- (Peroxisome Proliferator-activated Receptor, -isoform) PPAR- GST (Peroxisome Proliferator-activated Receptor, -isoform) PPAR--LBD (Peroxisome Proliferator-activated Receptor, a-isoform) PPAR--LBDGST (Peroxisome Proliferator-activated Receptor, a-isoform) PPAR- (Peroxisome Proliferator-activated Receptor, -isoform) PPAR-()-LBDGST (residues 165-441) (Peroxisome Proliferator-activated Receptor, -isoform, ligand binding domain) PPAR- (Peroxisome Proliferator-activated Receptor, -isoform) PPAR- -LBDGST (residues 204-477) (Peroxisome Proliferator-activated Receptor, -isoform, ligand binding domain) RAR- (Retinoic Acid Receptor, -isoform) RAR--LBD (residues 154-462) (Retinoic Acid Receptor, -isoform, ligand binding domain) RAR--LBDGST (residues 154-462) RAR--LBD (residues 173-409) (Retinoic Acid Receptor, -isoform, ligand binding domain) RAR--LBDGST (residues 173-409) RAR- (Retinoic Acid Receptor, -isoform)
PR-814
PR-816
10 g
Transcription Factors - Cell Extracts

Cat. No. Product HeLa cell nuclear extract (for in vitro transcription) Human HeLa cell nuclear extract (for in vitro pre-mRNA splicing) Human 293 cell nuclear extract (for in vitro pre-mRNA splicing) Human HeLa cell cytosol extract (S100) Human Qty 200 l 200 l 200 l 200 l
PR-815
10 g
PR-777 PR-778
PR-748
10 g PR-779 10 g PR-780
PR-822
PR-749
10 g
Hematopoietins
Cat. No. PR-693 Product BMP-4 (Bone Morphogenetic Protein-4) EPO (Erythropoietin a) EPO (Erythropoietin ) FLT3 Ligand (Fms-like Tyrosine Kinase 3 Ligand) FLT3 Ligand (Fms-like Tyrosine Kinase 3 Ligand) Murine, G-CSF(Granulocyte-Colony Stimulating Factor) Human, Recombinant, CHO Cells G-CSF(Granulocyte-Colony Stimulating Factor) Human, Recombinant, E. coli Qty 10 g 500 U 500 U 10 g 10 g 10 g 10 g
PR-823
10 g
PR-753 PR-819
5 g 10 g
PR-402 PR-403 PR-419
PR-824 PR-820
10 g 10 g PR-421 PR-424 10 g 5 g PR-423
PR-825 PR-755
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123
Hematopoetins / Cytokines
Hematopoietins (Contd.)
Cat. No. PR-425 PR-436 Product G-CSF, pegylated GM-CSF(Granulocyte Macrophage-Colony Stimulating Factor)Human, Recombinant, E. coli GM-CSF(Granulocyte Macrophage-Colony Stimulating Factor)Murine, Recombinant, E. coli IL-3 IL-11 LIF (Leukemia Inhibitory Factor) M-CSF (Macrophage Colony Stimulating Factor) SCF (Stem Cell Factor) SCF(Stem Cell Factor) Mouse, Recombinant, E. coli SCF (Stem Cell Factor) Rat, Recombinant, E. coli Qty 10 g 10 g
Hematopoetins/Cytokines
Suppressors
Cat. No. PR-407 PR-408 PR-463 Product BMP-2 (Bone Morphogenetic Protein-2) BMP-7 (Bone Morphogenetic Protein-7) IL-4 (Interleukin 4, B-cell stimulatory factor) Human IL-4 (Interleukin 4, B-cell stimulatory factor) Murine IL-10 (Interleukin 10) Insulin Leptin (Obesity Factor) Leptin (Obesity Factor) Murine, Recombinant, E. coli Leptin (Obesity Factor) Ovine (sheep), Recombinant, E. coli Leptin (Obesity Factor) Rat, Recombinant, E. coli Leptin, triple mutant (L39A, D40A, F41A) (Obesity Factor) Human, Recombinant, E. coli Leptin, quadruple mutant (L39A, D40A, F41A, I42A) (Obesity Factor) Human, Recombinant, E. coli Leptin, triple mutant (L39A, D40A, F41A) (Obesity Factor) Murine, Recombinant, E. coli Leptin, triple mutant (L39A, D40A, F41A) (Obesity Factor) Ovine (sheep), Recombinant, E. coli Leptin, quadruple mutant (L39A, D40A, F41A, I42A) (Obesity Factor) Ovine (sheep), Recombinant, E. coli Leptin, triple mutant (L39A, D40A, F41A) (Obesity Factor) Rat, Recombinant, E. coli Qty 10 g 10 g 10 g
PR-437 PR-462 PR-471 PR-490 PR-491 PR-682 PR-683 PR-684
10 g 10 g 10 g 10 g PR-470 10 g 10 g 10 g PR-483 10 g PR-482 PR-484 Qty 100 g 100 g 20 g 100 g 20 g PR-488 10 g 10 g 50 g PR-489 PR-486 PR-485 PR-477 PR-480 PR-481
PR-464
10 g
10 g 25 g 1 g 1 g 1 g 1 g 100 g
Antiviral Cytokines
Cat. No. PR-443 PR-439 PR-440 PR-441 PR-442 PR-444 PR-445 PR-420 Product IFN- 1b IFN- 2a IFN- 2a, pegylated IFN- 2b IFN- 2b, pegylated IFN- 1 IFN- 1b IFN-
100 g
100 g
100 g
100 g
Apoptosis-inducing Cytokines
Cat. No. PR-497 PR-498 PR-430 Product Prolactin Human Prolactin Ovine (sheep) TNF (Tumor Necrosis Factor) Human, Recombinant, E. coli TNF Mouse, Recombinant, E. coli TNF- Human, Recombinant, E. coli TPO (Thrombopoietin, Megakaryocyte colony stimulating factor) Human, Recombinant, E. coli TPO Mouse, Recombinant, E. coli Qty 50 g 1 mg 50 g
PR-487
100 g
PR-431 PR-685 PR-687
50 g 20 g 10 g
PR-688
10 g
124
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Cytokines Suppressors, Proinflammatory/ Growth Factors

Proinflammatory Cytokines
Cat No. PR-400 PR-401 PR-459 PR-460 PR-466 PR-467 PR-472 PR-473 PR-474 PR-478 PR-479 PR-430 PR-431 PR-685 Product IL-1 a Human, Recombinant, E. coli IL-1 Human, Recombinant, E. coli IL-1 Murine, Recombinant, E. coli IL-1ra (Interleukin 1 receptor antagonist) Human, Recombinant, E. coli IL-6 Human, Recombinant, E. coli IL-6 Murine, Recombinant, E. coli IL-15 Human, Recombinant, E. coli IL-15 Murine, Recombinant, E. coli IL-15 Rat, Recombinant, E. coli KGF-1 (Keratinocyte Growth Factor 1) Human, Recombinant, E. coli KGF-2 (Keratinocyte Growth Factor 2) Human, Recombinant, E. coli TNF Human, Recombinant, E. coli TNF Mouse, Recombinant, E. coli TNF- Human, Recombinant, E. coli Qty. 10 g 10 g 10 g PR-411 10 g 20 g 10 g 10 g 10 g 10 g 10 g 10 g 50 g 50 g 20 g PR-416 PR-417 PR-418 PR-422 PR-435 PR-433 PR-434 PR-414 PR-809 PR-415 PR-810 PR-413 PR-412
Cytokines/Growth Factors
Other Growth Factors

Cat No. PR-404 PR-405 Product Artemin BDNF (Brain-derived Neurotrophic Factor) CNTF (Ciliary Neurotrophic Factor) EG-VEGF (Endocrine Gland-derived Vascular Endothelial Growth Factor) EGF (Epidermal Growth Factor) Human, Recombinant, E. coli EGF (Epidermal Growth Factor) Human, Recombinant, Pichia pastoris FGF-1 (Acidic Fibroblast Growth Factor) Human, Recombinant, Sf9 insect cells FGF-1 (Fibroblast Growth Factor, acidic) Human, Recombinant, E. coli FGF-2 (Basic Fibroblast Growth Factor) Human, Recombinant, Sf9 insect cells FGF-2 (Fibroblast Growth Factor, basic) Human, Recombinant, E. coli FGF-2, bovine (Fibroblast Growth Factor, basic) Bovine, Recombinant, E. coli FGF-9 (Fibroblast Growth Factor-9) Murine, Recombinant, E. coli GDNF (Glial-Derived Neurotrophic Factor) Human, Rec., E. coli GH (Growth Hormone) Bream, Recombinant, E. coli GH (Growth Hormone) Ovine (sheep), Recombinant, E. coli GH (Growth Hormone) Rat, Recombinant, E. coli GH-20K, placental (Placental Growth Hormone-20K) GH-20K, pituitary (Pituitary Growth Hormone-20K) GH-22K (Placental Growth Hormone-22K) GH1, GH-22K (Pituitary Growth Hormone) HGF (Hepatocyte Growth Factor) Human, Recomb., High 5 insect cells IGF-I (Insulin Like Growth Factor I, IGF-1) Human, Recombinant, E. coli IGF-I Barramundi, Recombinant, E. coli IGF-I Bream, Recombinant, E. coli IGF-I Chicken, Recombinant, E. coli IGF-I Murine, Recombinant, E. coli Qty. 10 g 10 g 20 g 10 g
500 g 50 g 5 g 50 g 5 g 50 g 50 g 10 g 10 g 20 g 200 g 20 g 100 g 100 g 100 g 500 g 10 g 100 g 20 g 20 g 20 g 50 g
T-Cell Growth Factors

Cat No. PR-461 PR-463 PR-464 PR-468 PR-469 Product IL-2 Human, Recombinant, E. coli IL-4 Human, Recombinant, E. coli IL-4 Murine, Recombinant, E. coli IL-7 Human, Recombinant, E. coli IL-9 Human, Recombinant, E. coli Qty. 50 g 10 g 10 g 10 g 10 g
B-Cell Growth Factors

Cat No. PR-406 Product BLyS/BAFF (B Lymphocyte Stimulator/ B cellactivating factor belonging to the TNF family) Human, Recombinant, E. coli CD40L, soluble (CD40 Ligand/CD154/TRAP TNFrelated Activation Protein) Human, Recombinant, E. coli IL-5 Human, Recombinant, E. coli IL-6 Human, Recombinant, E. coli IL-6 Murine, Recombinant, E. coli Qty. 20 g PR-428 PR-427 PR-429 50 g PR-426 PR-438 10 g PR-446 PR-466 PR-467 20 g 10 g PR-451 PR-453 PR-449 PR-447
PR-680
PR-465
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125
Growth Factors - VEGF/Inhibitors/Ca2+ binding proteins

Other Growth Factors (Contd..)
Cat No. PR-452 PR-450 PR-448 PR-454 PR-455 PR-456 Product IGF-I Salmon/Trout, Recombinant, E. coli IGF-I Tuna, Recombinant, E. coli IGF-I Rat, Recombinant, E. coli IGF-II Human, Recombinant, E. coli IGF-II Chicken, Recombinant, E. coli IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) Human, Recombinant, mouse myeloma (NSO) cells IGFBP-2 Bovine, Recombinant, E. coli IGFBP-3 Human, Recombinant, E. coli KGF-1 (Keratinocyte Growth Factor 1) KGF-2 (Keratinocyte Growth Factor 2) NGF (Nerve Growth Factor ) NT-3, NGF-2 (Neurotrophin-3, Nerve Growth Factor 2) PDGF-AA (Platelet-derived Growth Factor-AA) PDGF-AB (Platelet-derived Growth Factor-AB) PDGF-BB (Platelet-derived Growth Factor-BB) VEGF (Vascular Endothelial Growth Factor) Human, Recombinant, E. coli VEGF (Vascular Endothelial Growth Factor) Bovine, Recombinant, E. coli VEGF (Vascular Endothelial Growth Factor) Mouse, Recombinant, E. coli VEGF-121 (Vascular Endothelial Growth Factor variant) Human, Recombinant, E. coli Qty. 20 g 20 g 20 g 50 g 20 g 25 g
Growth Factors
Growth Inhibitors
Cat. No. PR-492 Product MIA (Melanoma Inhibitory Activity Protein) Human, Recombinant, E. coli Qty
5 g
For TNF- and please view page #
Calcium binding proteins

Cat. No. PR-390 Product Calmodulin Human, Recombinant, E. coli S100A1 (Calcium-binding Protein A1) Human, Recombinant, E. coli S100A4 (Calcium-binding Protein A4) Human, Recombinant, E. coli S100A6 (Calcium-binding Protein A6) Human, Recombinant, E. coli S100A11 (Calcium-binding Protein A11) Human, Recombinant, E. coli Titin, I-band fragment Human, Recombinant, E. coli Ventricular Myosin Regulatory Light Chain LCII Human, Recombinant, E. coli Non-muscle Myosin II Regulatory Light Chain Human, Recombinant, E. coli Qty 100 g 50 g 5 g 5 g 10 g 100 g
PR-457 PR-458 PR-478 PR-479 PR-409 PR-493 PR-494 PR-495 PR-496
25 g 25 g
PR-391 PR-1258
10 g 10 g 20 g PR-1265 10 g PR-392 10 g PR-393 10 g 10 g PR-394 PR-1260
100 g 100 g
PR-689
10 g
Antioxidants
Cat. No. PR-150 Product Cu/Zn SODHis (Cu/Zn Superoxide Dismutase) Mn SODHis (Manganese Superoxide Dismutase) MSRA (Methionine sulfoxide reductase A, EC1.8.4.6) MSRB1 (Methionine sulfoxide reductase B1, EC1.8.4.6) GST Glutathione S-Transferase Schistosoma japonicum, Recombinant, E. coli
His His
PR-691
10 g
Qty 1000 U 500 U
PR-692
10 g
PR-149 PR-130
PR-690
10 g
100 g
PR-131
100 g
PR-811
5 g
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Fmoc and Boc Amino/Amino Acid catridges

Fmoc Labeled amino acids
Fmoc Amino Acids Ala Fmoc Amino Acids Abu Fmoc Amino Acids Arg Fmoc Amino Acids Asn Fmoc Amino Acids Asp Fmoc Amino Acids Cys Fmoc Amino Acids Glu Fmoc Amino Acids Gln Fmoc Amino Acids Gly Fmoc Amino Acids His Fmoc Amino Acids Ile Fmoc Amino Acids Leu Fmoc Amino Acids Lys Fmoc Amino Acids Met Fmoc Amino Acids Nle Fmoc Amino Acids Nva Fmoc Amino Acids Orn Fmoc Amino Acids Phe Fmoc Amino Acids Pro Fmoc Amino Acids Ser Fmoc Amino Acids Thr Fmoc Amino Acids Tyr Fmoc Amino Acids - Val Fmoc Amino Acids Unusual
PEPTIDE SYNTHESIS
Amino acids
Amino Acid Cartridges Fmoc L-Amino Acid Cartridges for ABI Synthesizers (1 mmol)
Available in 1 & 10 Cartridges pack size (Enquire for Pricing)
Fmoc D-Amino Acid Cartridges for ABI Synthesizers (1 mmol)

Available in 1 & 10 Cartridges pack size (Enquire for Pricing)
Fmoc L-Amino Acid Cartridges for Rainin PS-3 Synthesizers (1 mmol)

Available in 10 Cartridges pack size (Enquire for Pricing)
Fmoc L-Amino Acid Cartridges for Rainin PS-3 Synthesizers (0.4 mmol)
Available in 10 Cartridges pack size (Enquire for Pricing)
Boc L-Amino Acid Cartridges for ABI Synthesizers (1 mmol)

Available in 10 Cartridges pack size (Enquire for Pricing).
Boc D-Amino Acid Cartridges for ABI Synthesizers (1 mmol)

Available in 1 & 10 Cartridges pack size (Enquire for Pricing).
Boc Labeled Amino Acids

Boc Amino Acids Ala Boc Amino Acids Arg Boc Amino Acids Asn Boc Amino Acids Asp Boc Amino Acids Cys Boc Amino Acids Glu Boc Amino Acids Gln Boc Amino Acids Gly Boc Amino Acids His Boc Amino Acids Ile Boc Amino Acids Leu Boc Amino Acids Lys Boc Amino Acids - Met Boc Amino Acids - Nle Boc Amino Acids Nva Boc Amino Acids - Orn Boc Amino Acids - Phe Boc Amino Acids - Pro Boc Amino Acids - Ser Boc Amino Acids - Trp Boc Amino Acids Tyr Boc Amino Acids Val Boc Amino Acids Unusual
Boc L-Amino Acid Cartridges for ABI Synthesizers (2 mmol)

Miscellaneous Amino Acid Cartridges for ABI Synthesizers (1 mmol)

Available in 1g, 5g and 25g pack sizes. Please enquire for pricing.
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Resins/ Coupling Labeling Reagents

RESINS 2-Chlorotrityl Resins
Available in 1g & 5g pack sizes of different Amino Acids (Enquire for Pricing)
PEPTIDE SYNTHESIS
Resins and reagents
Coupling Reagents and Additives

Cat. No. 21663 Product Benzotriazole-1-yl-oxy-trispyrrolidino-phosphonium hexafluorophosphate Benzotriazole-1-yl-oxy-trispyrrolidino-phosphonium hexafluorophosphate Benzotriazole-1-yl-oxy-trispyrrolidino-phosphonium hexafluorophosphate BOP DCC DCC DIC DIC DIC DIEA DIEA in NMP (2.0 M) DIBOC DIBOC DIBOC DMAP DMAP DMAP DMAP EDC EDC EDC HBTU HBTU HBTU in HOBt (0.5 M) HOAt HOAt HOBt, anhydrous HOBt, anhydrous Piperidine Piperidine TBTU TBTU Qty 5g
Trityl Resins
Available in 1g, 5g & 25 g of pack size (Enquire for Pricing) Diaminobutane trityl resin Trityl chloride resin 4-Methoxytrityl chloride resin Cysteamine 4-methoxytrityl resin Thiol 4-methoxytrityl resin
21665
25 g
21667
100 g
23510 29860 29861 25113 25114 25115 27221 27220 23740 23741 23742 29883 29884 29858 29859 29855 29856 29857 21001 21002 27217 27256 27255 21003 21004 27218 27219 20376 20377
25 g 100 g 500 g 5g 25 g 100 g 500 mL 200 mL 5g 25 g 100 g 5g 25 g 100 g 500 g 5g 25 g 100 g 100 g 500 g 200 mL 1g 5g 100 g 500 g 200 mL 500 mL 100 g 500 g
Highly Substituted Resins

Merrifield resin MBHA resin Fmoc-Rink Amide MBHA Resins.
HMP Resins
Available in 1g & 5g pack sizes (Enquire for pricing)
MAPS Resins
Fmoc and Boc resins available in 8-branch and 4-branch formats.
PAM Resins
Available in 1g, 5g & 10g of pack sizes (Enquire for pricing).
TentaGel Resins
Available in 1g & 5g of pack sizes (Enquire for pricing).
Wang Resins
Available in 1g, 5g & 10g of pack sizes (Enquire for pricing).
AFC, AMC, and pNA Resins

Available in 0.5g, & 1g of pack sizes (Enquire for pricing).
Miscellaneous Resins
Available in 0.5g, 1g & 5g of pack sizes (Enquire for types and pricing).
Miscellaneous Reagents
Cat. No. 20814 20815 20816 27257 27258 21392 24616 25241 Product Fmoc-OSu Fmoc-OSu Fmoc-OSu HMPA (HMP Linker) HMPA (HMP Linker) Rink Amide Linker Rink Amide Linker Ninhydrin Test Kit Qty 5g 25 g 100 g 1g 5g 1g 5g 1 each
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Building Blocks
Building Blocks
Cat. No. 29271 29272 29273 29274 29275 29276 29277 29278 29279 29280 29281 29282 29283 29284 29285 29286 29287 29288 29289 29290 29291 29292 29293 29294 29295 29296 29297 29298 29299 29300 29301 29302 29315 29316 29319 29320 29321 29322 29323 29324 Product ()-N-4-Boc-2-methylpiperazine ()-N-4-Boc-2-methylpiperazine tert-Butyl N-(2-aminoethyl)carbamate tert-Butyl N-(2-aminoethyl)carbamate tert-Butyl 1-piperazinecarboxylate tert-Butyl 1-piperazinecarboxylate (S)-N-4-Benzyl-2-isobutylpiperazine (S)-N-4-Benzyl-2-isobutylpiperazine (S)-N-1-Boc-2-isobutylpiperazine (S)-N-1-Boc-2-isobutylpiperazine (S)-N-4-Benzyl-2-isopropylpiperazine (S)-N-4-Benzyl-2-isopropylpiperazine (S)-N-1-Boc-2-isopropylpiperazine (S)-N-1-Boc-2-isopropylpiperazine (R)-N-4-Benzyl-2-phenylpiperazine (R)-N-4-Benzyl-2-phenylpiperazine (R)-N-4-Benzyl-2-(benzyloxymethyl) piperazine (R)-N-4-Benzyl-2-(benzyloxymethyl) piperazine (R)-N-1-Boc-2-(benzyloxymethyl)piperazine (R)-N-1-Boc-2-(benzyloxymethyl)piperazine (S)-N-4-Benzyl-2-benzylpiperazine (S)-N-4-Benzyl-2-benzylpiperazine (S)-N-1-Boc-2-benzylpiperazine (S)-N-1-Boc-2-benzylpiperazine (S)-N-4-Benzyl-2-(methylthioethyl)piperazine (S)-N-4-Benzyl-2-(methylthioethyl)piperazine (S)-N-4-Benzyl-2-(3-indolylmethyl)piperazine (S)-N-4-Benzyl-2-(3-indolylmethyl)piperazine (S)-1,4-Diazabicyclo[4.3.0]nonane (S)-1,4-Diazabicyclo[4.3.0]nonane ()-1,4-Diazabicyclo[4.4.0]decane ()-1,4-Diazabicyclo[4.4.0]decane trans-4-Hydroxy-L-proline methyl ester hydrochloride salt trans-4-Hydroxy-L-proline methyl ester hydrochloride salt (R)-(-)-2-Hydroxy-4-phenylbutyric acid (R)-(-)-2-Hydroxy-4-phenylbutyric acid (1S,3R)-Methyl hydrogen cis-1,3cyclopentanedicarboxylate (1S,3R)-Methyl hydrogen cis-1,3cyclopentanedicarboxylate 4-Amino-2-bromopyrimidine-5-carbonitrile 4-Amino-2-bromopyrimidine-5-carbonitrile Qty 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 1g 5g 5g 60016-5 25 g 5g 25 g 5g 60016-1 60015-5 60013-5 60014-1 60014-5 60015-025 N-1-Boc-4-aminopiperidine 60010-1 60010-5 60011-5 60011-25 60012-1 60012-5 60013-1 29329 29330 29327 29328 29326 29325 29315
PEPTIDE SYNTHESIS
Building Blocks
Building Blocks
Cat. No. 29325 Product 4-Amino-3-iodo-1H-pyrazolo [3,4-d]pyrimidine trans-4-Hydroxy-L-proline methyl ester hydrochloride salt 4-Amino-3-iodo-1H-pyrazolo[3,4-d] pyrimidine 4-Amino-3-iodo-1H-pyrazolo[3,4-d] pyrimidine 2-Chloro-4,6-dimethoxy-1,3,5-triazine (98%) 2-Chloro-4,6-dimethoxy-1,3,5-triazine (98%) a- (p-Toluenesulfonyl)-4-fluorobenzylisonitrile a - (p-Toluenesulfonyl)-4fluorobenzylisonitrile 3-tert-Butoxycarbonylaminopiperidine 3-tert-Butoxycarbonylaminopiperidine 4-tert-Butoxycarbonylaminopiperidine 4-tert-Butoxycarbonylaminopiperidine N-1-Boc-3-aminopiperidine N-1-Boc-3-aminopiperidine N-1-Boc-4-aminopiperidine Qty 1g
5g
1g
5g
25 g 25 g 1g 5g
1g 5g 5g 25 g 1g 5g 1g 5g 1g 5g 0.25 g
N-Boc-1,2,3,6-tetrahydropyridine N-Boc-1,2,3,6-tetrahydropyridine N-Boc-3-hydroxy-1,2,3,6tetrahydropyridine N-Boc-3-hydroxy-1,2,3,6tetrahydropyridine N-Boc-3-hydroxy-1,2,3,6tetrahydropyridine N-Boc-trans-4-bromo-3-hydroxypiperidine N-Boc-trans-4-bromo-3-hydroxypiperidine
60015-1
1g
5g 1g 5g
25 g 5g 25 g
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Bioactive Peptides
Amyloid Peptides Adrencorticotrophin peptides Agouti Related Peptides Angiotensins and Related Peptides Antibiotic Peptides Bombesins Osteocalcins Bradykinins Cell Permeable Peptides Calcitonin Related Peptides Endomorphins Cholecystokinin-Pancreozymin Endorphins Enkaphalins Corticotropin Releasing Factor Dynorphins Endothelins Leutenizing Hormone Endexins HIV Related Peptides Galanins Gastrins Fibronectin Fragments Glucagon-Like Peptides Growth Hormone Releasing Factors Matrix Metalloproteinases Neurokinins Natriuretic Peptides Orexins Neuromedins Neuropeptide Y and Analogs Neurotensins and Related Peptides Oxytocins, Vasopressins, and Related Peptides Pancreatic Polypeptides Parathyroid Hormones and Related Peptides Toxins Peptide YY and Analogs Prolactin Releasing Peptides Pituitary Adenylate Cyclase Activating Peptides (PACAPS) Proteolipid Proteins (PLPs) Secretins Somatostatins Substance P and Analogs Thrombin Related Peptides Thyrotropin Releasing Hormones and Related Peptides TNF Peptides Vasoactive Intestinal Peptides (VIPs) Viral Peptides Miscellaneous Bioactive Peptides Hepatitis-C Related Peptides Phosphopeptides Protein Kinase Related Peptides Apoptosis Blocking Peptides
PEPTIDE SYNTHESIS
Catalogue Peptides
Cancer Research Blocking Peptides Signal Transduction Blocking Peptides
We have a broad range of Bioactive peptides for the categories listed. Please inquire for your specific requirement ! If not catalogued, we can custom synthesize it for you! For Custom Peptide Synthesis please view page # 133
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Gene Synthesis, Library construction

Customized Gene Synthesis
We offer custom gene synthesis service for a very economical prices. Turnaround time is dependent on sequence length. Progress report will be issued at different stages. Confidentiality will be assured through a non-disclosure agreement (if necessary). The following are included: Artificial gene synthesis; Expression optimization; Codon optimization (if necessary); Insertion of gene into standard plasmid or customerprovided vector; Double stranded DNA sequencing data with chromatogram; The final product (10g) can be delivered as a vector DNA construct (vector map included) and/or as a lyophilized clone mixture 100% fidelity Supplied as lyophilized plasmid E.coli culture transformed and stab provided at minimal cost Expression in E.coli, Pichia pastoris, Baculovirus
CUSTOM SERVICES
Genomic Services
Construction of a Genomic DNA Lambda library

We can clone large inserts ranging in size from 9-23 kb into a lamdba vector. The Lambda DASH II Vector (Stratagene) will be used for genomic lambda library construction. We do not guarantee average insert size but will attempt to obtain desired range of average insert size. Insert size can vary from about 9-23Kb, as specified in manufactures kit. We guarantee the minimum requested 1,000,000 clones. Genomic library preparation highlights: DNA purification (>100kb in average). DNA random shearing by enzymatic methods. Size fractionation. Sticky-end ligation to the vector. Packaging and stock preparation. Customer will receive non-amplified phage stocks. Recombinant clones will be up to about 1 million.
Construction of a Lambda cDNA library

cDNA libraries are constructed using our proprietary technology, which features full-length enriched, high quality size-fractionated, and directionally cloned cDNA. We do not guarantee average insert size but will attempt to obtain desired range of average insert size. We guarantee the minimum requested 1,000,000 clones. cDNA library preparation highlights: Total and polyA RNA isolation. 1st strand cDNA synthesis: 5 adaptor primers will be added to full-length cDNAs during this step; an oligo-dT or a random primer based on the need of customer will be used. 2nd strand cDNA synthesis by primer extension. Double stranded cDNAs will be amplified using low cycling PCR if necessary (optional). Size fractionation (normally recombinant clones will contain an average insert size of at least 1kb). Ligation to phage vector. Packaging. Quality control (average insert size and non-insert clone determination, number and percentage of recombinants). Customer will receive non-amplified cDNA stocks. Recombinant clones will be up to about 1 million for lambda. Library amplification is available, however not included in this offer.
BAC genomic library

BAC libraries are essential elements in doing large-scale genome research such as genome sequencing, physical maps, and gene cloning. Bacterial artificial chromosomes (BAC) cloning technology was developed in early 1990s and quickly gained popularity. This technology was used in applications for genomic analysis that included large-scale physical mapping and genomic sequencing although this technology was developed much later than Yeast Artificial Chromosome (YAC) and cosmid cloning technologies. We guarantee coverage. We perform HMW DNA extraction ourselves. We have experience with plant, fungal, bacterial and animal libraries. We have extensive experience with GC-rich organisms and marine bacteria. Our arrayed BAC libraries are shipped in bar-coded microplates. Shipment temperature of non-arrayed libraries is always monitored using a BLUE FLAG Temperature Data logger. We provide technical support.
A few libraries constructed in the past include: Starting material Mouse tissue Soy bean Zeas Mays Beetle Canola plant Pseudomonas sp. Human liver P. brassicacearum P . omnivora Insert size 180 Kb 150 Kb 150 Kb 162 Kb 130 Kb 140 Kb 215 Kb 160 Kb 185 Kb
Plasmid DNA library (shotgun)

A modified pBluescript vector will be used. We do not guarantee average insert size but will attempt to obtain desired range of average insert size. We guarantee the minimum requested 500,000 clones. Service highlights: DNA purification (>100kb in average). DNA random shearing by chemical methods. Size fractionation. Blunt-end ligation to the vector. Recombinant clones will be up to about 500,000.
Clone array is optional.
Arrayed, Non-arrayed, and screened libraries available.
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Library Construction/ Site-directed Mutagenesis/Genome Walking

Plasmid cDNA library
cDNA libraries are produced using proprietary technology, which features full-length enriched, high quality size-fractionated, and directionally cloned cDNA. We do not guarantee average insert size but will attempt to obtain desired range of average insert size. We guarantee the minimum requested 500,000 clones. Service highlights: Competent cell preparation. cDNA synthesis to obtain full-length enriched cDNAs. Cloning will be directional. Size fractionation and ligation to vector (modified pBluescript). Transformation in E. coli DH10B. Plate will be identified with bar-coded labels. OPTIONAL: Arraying and Normalization Fluoresceins DAPI Hoechst Nucleotides Propidium Acridines Verapamil Biamines Ryanodines Rhodamines Phospholipids Ceramides Peptides Glycosides Tetradotoxins Bungarotoxins Taxols
CUSTOM SERVICES
Genomic & Proteomic Services
The fluorescent probes/dyes available are: Fluoresceins FMA/FITC JOE/HEX/TET FDG/ FDP BCECF Luciferins Coelenterazines Dioxetanes BAPTA Rhodamines TAMRA/ROX Rh110/Rh123 Resorufin Carbocyanines JC-1/Dil/Dio DiBAC/DiSBAC Indo/Fura EGTA Coumarins AFC/AMC Psoralens SPQ/MEQ NBD Lucifer Yellow Acrylodan ATP/dUTP AMP/GMP Coumarins Transferrin Phycoerythrins Avidins Biotins Antibodies Microspheres Phalloidins DNA/RNA
Site-directed mutagenesis
perform the site-directed mutagenesis and subsequent sequence verification; provide purified plasmid DNA; provide E.coli strain harboring the plasmid with the target mutation; provide a complete mutagenesis report.
Neuropeptides
Please provide us with 1mg of plasmid DNA with the target sequence, plasmid map, and last but not least, target sites for mutations! Plasmid must be less than 8Kb. If greater than 8Kb a subcloning fees shall be charged extra.
Custom Synthesis of Amino Acids and Peptides

We routinely perform difficult peptide synthesis such as synthesis of long peptides (>50-mers), peptides containing unusual modifications and cyclic peptides. In addition, we can also do large-scale synthesis (>100 g). Optimized Fmoc and Boc methodologies are employed in house to obtain high quality peptides. Although most of the peptides are purified by HPLC using reverse phase C 4 and C 18 columns, alternative purification methods (e.g. ion exchange and gel filtration chromatography) are used to purify some unique peptides. All custom peptides are accompanied with MS and HPLC analyses. Additional analytical data (e.g., sequencing, amino acid analysis) are available upon request. We can synthesize modified peptides such as: DNA-peptide conjugates Drug-peptide conjugates Glycopeptides Lipopeptides Phosphopeptides Cyclic peptides (N -> C) Peptides with disulfide bonds Pegylated peptides Biotinylated peptides MAPS peptides Dye-labeled peptides: Peptides with C-terminal modification: Peptides with reduced amide bond Peptides labeled with C 13 or N 15 Peptide libraries
Other services:
Subcloning Gap filling 5 and 3 RACE of first-strand cDNAs 5 and 3 genomic walking Identifying intron/exon junctions Identifying transgene/genomic DNA junctions Identifying gene traps and transposon-insertion location Bi-directional sequence extension of STSs and EST
Custom Synthesis of Fluorescent Probes

We have extensive experience in custom-synthesizing biological stains, fluorescent and luminescent proves, peptides, nucleotides, carbohydrates and other biological molecules. With our state-of the art synthetic and analytical instruments and GMP facilities, you can be assured that you would get high quality products from us. We offer custom conjugations for labeling your peptides, antibodies, proteins, nucleotides and nucleic acids with fluorescent dyes, luminescent probes, biotin, enzymes or other tagging molecules.
Quantity range from 5mg to 100 mg Purity levels range from Crude to >98% Please enquire for bulk pricing GMP-Grade Peptides We synthesize GMA-grade peptides through our principals; AnaSpec Inc., USA. Documentation and regulatory support are provided for your clinical applications. AnaSpec holds a FDA Registration for GMP Drug Manufacturing (Registration #2951078).
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Custom Production of Monoclonal and Polyclonal Antibodies

Polyclonal Antibody Production
Polyclonal Antibody Production for 2 rabbits: NIH 90-day standard protocol or 70-day conventional protocol 10 mL pre-immune serum 100 mL anti-serum
CUSTOM SERVICES
Antibody and Other Services
Phase II: Cell Fusion and Parental Clone Screening (3 wks) Mouse with the highest titer given final boost to establish murine myeloma cell line Fused cell clones screened by ELISA to select positive antibody-secreting parental clones Potential parental clones expanded into 24-well plates for harvest of tissue culture supernatant for further antibody screening (ELISA, Western Blot, or IHC) Shipment of 5-12 supernatants (2 mL/clone)
Additional antibody services include: ELISA Peptide Synthesis 10 mg peptide, >80% purity, MS and HPLC certified Peptide-Carrier Protein Conjugation to BSA or KLH
Phase III: Monoclonal Selection and Cryogenic Vial Preservation (3 wks) Monoclonal selection performed with 1-5 parental clones Subclone with high specificity expanded and cryopreserved (4-5 vials each) Shipment of hybridoma cells and up to 10 mL of supernatant from each subclone Phase IV: Large Scale Antibody Production (3 wks) *Production performed either in vivo (ascites) or in vitro (bioreactor, stationary cell culture).
Complete Anti-Peptide Antibody Package includes:

Free consultation for selection of epitope 5 mg purified (>80% purity, up to 15 amino acids) peptide delivered to researcher for immediate study 1 mg conjugated to BSA for ELISA screening; 4 mg conjugated to KLH for antibody production Immunization of rabbits, mice, or chickens at your preference Peptide-specific ELISA titration of antibody for all bleeds Pre-immune samples of serum, ascites, or eggs Shipment of 100 mL anti-serum, 20 mL ascites, or 12 antibody-containing eggs
Cell-Based Assays
There are a variety of fluorescent dyes for monitoring cell functions. Using our superior fluorescent dyes, the following cell-based assays are provided: Cytotoxicity Cell Proliferation Cell viability Cell adhesion Muti-drug resistance
Monoclonal Antibody Production

Shipment of 100 mL anti-serum, 20 mL ascites, or 12 antibodycontaining eggs Free consultation for epitope selection Evaluation of suitable screening methods Frequent interactions between researcher and AnaSpec during hybridoma development process Project segmentation into different phases to provide researcher flexibility for terminating project at the end of each phase Full confidentiality assured
Analytical Services
To accelerate customers research and development projects, we offer a variety of analytical services besides our specialized custom services described above. Small biomolecules Proteins Oligosaccharides Oligonucleotides Glycoproteins Lipids Other biopolymers
Phase I: Immunogen Design and Immunization (9 wks) Design and preparation of protein antigen by researcher or peptide immunogen Site-directed conjugation for peptide including peptide-KLH as antigen and peptide-BSA as screening reagent Immunization of 6 Balb/C mice, including maintenance and 3 immunizations for a standard 60-day protocol Determination and qualification of titer of test bleed (e.g., ELISA, Western Blot, IP) Shipment of all 6 mice test bleed at 1:100 dilution (1 mL each)
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Reactive Fluorescent Dyes

Fluorescent Dyes
Cat. No. 81401 81207 Product ABD-F, [4-Fluoro-7-aminosulfonyl benzofurazan] abs : 315 nm; em: none AMCA-X, [6-((7-Amino-4-methylcoumarin3-acetyl)amino)hexanoic acid] abs : 353 nm; em: 442 nm AMCA-X, SE , [6-((7-Amino-4-methyl coumarin-3-acetyl)amino)hexanoic acid, succinimidyl ester] abs : 442 nm; em: 354 nm 2-Aminoacridone abs : 425 nm (pH.7); em: 532 nm (pH.7) AMF, 4'-(Aminomethyl)fluorescein, hydrochloride abs : 493 nm; em: 516 nm 1-Aminomethylpyrene, hydrochloride abs : 340 nm; em: 376 nm ANDS, [7-Aminonaphthalene-1,3-disulfonic acid, potassium salt] abs : 350 nm; em: 450 nm ANTS, [8-Aminonaphthalene-1,3,6-trisulfonic acid, disodium salt] *UltraPure Grade* abs : 353 nm; em: 520 nm APTS, [8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt] abs : 424 nm; em: 505 nm Badan, [6-Bromoacetyl-2-dimethyl amino naphthalene] abs : 387 nm; em: 520 nm bBBr, [Dibromobimane] *UltraPure Grade* abs : 395 nm; em: 490 nm mBBr, [Monobromobimane] *UltraPure Grade* abs : 395 nm; em: 490 nm 5-(and-6)-Carboxy-2',7'-dichlorofluorescein abs : 504 nm; em: 529 nm 5-(and-6)-Carboxy-2',7' dichloro fluorescein, succinimidyl ester abs : 504 nm; em: 529 nm 5(6)-CR110, [5-(and-6)-Carboxyrhodamine 110, hydrochloride] abs : 498 nm; em: 521 nm 5-CR110, [5-Carboxyrhodamine 110, hydrochloride] abs : 498 nm; em: 521 nm 6-CR110, [6-Carboxyrhodamine 110, hydrochloride] abs : 498 nm; em: 521 nm 5(6)-CR110, SE, [5-(and-6)Carboxyrhodamine 110, succinimidyl ester] abs : 498 nm; em: 521 nm 5-CR110, SE, [5-Carboxyrhodamine 110, succinimidyl ester] abs : 498 nm; em: 521 nm 6-CR110, SE, [6-Carboxyrhodamine 110, succinimidyl ester] abs : 498 nm; em: 521 nm 5(6)-CR6G, [6-Carboxyrhodamine 6G, hydrochloride] abs: 519 nm; em: 544 nm 5-CR6G, [5-(and-6)-Carboxyrhodamine 6G, hydrochloride] abs: 518 nm; em: 544 nm 6-CR6G, [5-Carboxyrhodamine 6G, hydrochloride] abs: 520 nm; em: 547 nm Qty 10 mg 25 mg
FLUORESCENCE
Fluorescent Dyes
Fluorescent Dyes (Contd.)

Cat. No. 81104 81105 81106 81402 25 mg 81216 25 mg 81403 100 mg 81501 1g 81201 Product 5(6)-CR6G, SE, [5-(and-6)-Carboxyrhodamine 6G, succinimidyl ester] abs: 522 nm; em: 550 nm 5-CR6G, SE, [5-Carboxyrhodamine 6G, succinimidyl ester] abs: 524 nm; em: 556 nm 6-CR6G, SE, [6-Carboxyrhodamine 6G, succinimidyl ester] abs: 524 nm; em: 551 nm DACIA, [N-(7-Dimethylamino-4-methylcoumarin3-yl)iodoacetamide] abs: 376 nm; em: 465 nm DACITC, [7-Dimethylamino-4-methylcoumarin3-isothiocyanate] abs: 400 nm; em: 476 nm DACM, [N-(7-Dimethylamino-4-methylcoumarin3-yl)maleimide] abs: 383 nm; em: 463 nm Dansyl cadaverine, [5-Dimethylaminonaphthalene -1-(N-(5-aminopentyl))sulfonamide] abs: 333 nm; em: 518 nm Dansyl chloride [5-Dimethylaminonaphthalene1-sulfonyl chloride], abs: 372 nm; em: none Dansyl-X, acid abs: 333 nm; em: 518 nm Dansyl-X, SE abs: 333 nm; em: 518 nm Qty 10 mg 5 mg 5 mg 10 mg 10 mg 10 mg 25 mg
81208
10 mg
81513 81512 81522 81529
81528
100 mg
100 mg
81523
10 mg
81209 81214
100 mg 25 mg 25 mg
81420 81448 81433 81015 81016
10 mg 25 mg 25 mg 100 mg 25 mg
81422
DCIA, [7-Diethylamino-3-((4'-(iodoacetyl) amino)phenyl) -4-methylcoumarin] abs: 384 nm; em: 470 nm DEAC, acid, [7-Diethylaminocoumarin3-carboxylic acid] abs: 432 nm; em: 472 nm DEAC, SE, [7-Diethylaminocoumarin-3carboxylic acid, succinimidyl ester] abs: 432 nm; em: 472 nm DMACA, [7-Dimethylaminocoumarin4-acetic acid] abs: 370 nm; em: 459 nm DMACA, SE, [7-Dimethylaminocoumarin4-acetic acid, succinimidyl ester] abs: 376 nm; em: 468 nm 5-DTAF, [5-(4,6-Dichlorotriazinyl) aminofluorescein] abs: 492 nm; em: 517 nm 6-DTAF [6-(4,6-Dichlorotriazinyl) aminofluorescein] abs: 492 nm; em: 517 nm EDANS, acid, [5-((2-Aminoethyl)amino) naphthalene-1-sulfonic acid] abs: 335 nm; em: 493 nm EDANS Iodoacetamide, [5-((((2-Iodoacetyl)amino)ethyl)amino) naphthalene-1-sulfonic acid] abs: 336 nm; em: 490 EDANS C2 maleimide abs: 336 nm; em: 490 EDANS, sodium salt, [5-((2 Aminoethyl)amino)naphthalene-1-sulfonic acid, sodium salt] abs: 335 nm; em: 493 5(6)-FAM, [5-(and-6)-Carboxyfluorescein] abs: 494 nm (pH>7.0); em: 519 nm (pH>7.0)
81210 81211
250 mg 25 mg
81225 81226
100 mg 25 mg
81131
25 mg 81001 5 mg 5 mg 5 mg 23887 5 mg 81431 5 mg 81432 25 mg 10 mg 81002 10 mg 81535 81012
25 mg
81132 81133 81134
25 mg
1g
81135
100 mg
81136
25 mg 1g
81101 81102 81103
250 mg
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Fluorescent Dyes (Contd.)
Cat. No. 23887 Product EDANS, acid, [5-((2-Aminoethyl) amino)naphthalene-1-sulfonic acid] abs: 335 nm; em: 493 nm EDANS Iodoacetamide, [5-((((2-Iodoacetyl)amino)ethyl)amino) naphthalene-1-sulfonic acid] abs: 336 nm; em: 490 EDANS C2 maleimide abs: 336 nm; em: 490 EDANS, sodium salt, [5-((2-Aminoethyl)amino) naphthalene-1sulfonic acid, sodium salt] abs: 335 nm; em: 493 5-FAM, [5-Carboxyfluorescein] abs: 492 nm (pH>7.0); em: 518 nm (pH>7.0) 6-FAM, [6-Carboxyfluorescein] abs: 495 nm (pH>7.0); em: 517 nm (pH>7.0) 5-FAM cadaverine, [Fluorescein-5-carboxamide cadaverine] abs: 494 nm (pH>7.0); em: 521 nm (pH>7.0) 5-FAM LYS, [Fluorescein-5-carboxamide lysine] abs: 494 nm (pH>7.0); em: 521 nm (pH>7.0) 5(6)-FAM, SE, [5-(and-6)Carboxyfluorescein, succinimidyl ester] abs: 494 nm (pH>7.0); em: 519 nm (pH>7.0) 5-FAM, SE, [5-Carboxyfluorescein, succinimidyl ester] abs: 492 nm (pH>7.0); em: 518 nm (pH>7.0) 6-FAM, SE, [6-Carboxyfluorescein, succinimidyl ester] abs: 495 nm (pH>7.0); em: 517 nm (pH>7.0) 5-FAM-X, SE, [6-(Fluorescein-5carboxamido)hexanoic acid, succinimidyl ester] abs: 494 nm (pH>7.0); em: 521 nm (pH>7.0) 5-FITC cadaverine, [5-((5-Aminopentyl)thioureidyl)fluorescein] abs: 492 nm (pH>7.0); em: 516 nm (pH>7.0) FITC Isomer I, [5-FITC; fluorescein-5-isothiocyanate] abs: 494 nm (pH>7.0); em: 519 nm (pH>7.0) FITC Isomer II, [6-FITC, fluorescein-6-isothiocyanate] abs: 494 nm (pH>7.0); em: 520 nm (pH>7.0) Fluorescamine *UltraPure Grade* abs: 315 nm; em: None Fluorescein-5-maleimide abs: 493 nm; em: 515 nm 5-FTSC, [Fluorescein-5-thiosemicarbazide] abs: 492 nm (pH>7.0); em: 516 nm (pH>7.0) 6-HEX, acid, [6-Carboxy-2',4,4',5',7,7'hexachlorofluorescein] abs: 533 nm; em: 550 nm 6-HEX, SE, [6-Carboxy-2',4,4',5',7,7'hexachlorofluorescein, succinimidyl ester] abs: 533 nm; em: 550 nm HiLyte Fluor 488 acid (can be substituted for FITC) abs: 497 nm; em: 525 nm HiLyte Fluor 555 acid (can be substituted for Cy3 dye) abs: 550 nm; em: 566 nm HiLyte Fluor 647 acid (can be substituted for Cy5 dye) abs: 650 nm; em: 675 nm Qty 1g
FLUORESCENCE
Fluorescent Dyes
Fluorescent Dyes (Contd..)

Cat. No. 81260 81265 100 mg 81161 25 mg 1g 81256 100 mg 81261 100 mg 10 mg 81266 Product HiLyte Fluor 680 acid (can be substituted for Cy5.5 dye) abs: 678 nm; em: 699 nm HiLyte Fluor 750 acid (can be substituted for Cy7 dye) abs: 753 nm; em: 778 nm HiLyte Fluor 488 acid, SE (can be substituted for FITC) abs: 498 nm; em: 525 nm HiLyte Fluor 555 acid, SE (can be substituted for Cy3 dye) abs: 552 nm; em: 569 nm HiLyte Fluor 647 acid, SE (can be substituted for Cy5 dye) abs: 649 nm; em: 674 nm HiLyte Fluor 680 acid, SE (can be substituted for Cy5.5 dye) abs: 678 nm; em: 699 nm HiLyte Fluor 750 acid, SE (can be substituted for Cy7 dye) abs: 754 nm; em: 778 nm HiLyte Fluor 488 amine (can be substituted for FITC) abs: 499 nm; em: 526 nm HiLyte Fluor 555 amine (can be substituted for Cy3 dye) abs: 551 nm; em: 567 nm HiLyte Fluor 647 amine (can be substituted for Cy5 dye) abs: 649 nm; em: 674 nm HiLyte Fluor 680 amine (can be substituted for Cy5.5 dye) abs: 678 nm; em: 699 nm HiLyte Fluor 750 amine (can be substituted for Cy7 dye) abs: 754 nm; em: 778 nm HiLyte Fluor 488 C2 maleimide (can be substituted for FITC) abs: 498 nm; em: 525 nm HiLyte Fluor 555 C2 maleimide (can be substituted for Cy3 dye) abs: 552 nm; em: 569 nm HiLyte Fluor 647 C2 maleimide (can be substituted for Cy5 dye) abs: 649 nm; em: 674 nm HiLyte Fluor 680 C2 maleimide (can be substituted for Cy5.5 dye) abs: 678 nm; em: 699 nm HiLyte Fluor 750 C2 maleimide (can be substituted for Cy7 dye) abs: 754 nm; em: 778 nm HiLyte Fluor 488 hydrazide (can be substituted for HRP) abs: 499 nm; em: 526 nm HiLyte Fluor 555 hydrazide (can be substituted for Cy3 dye) abs: 551 nm; em: 567 nm HiLyte Fluor 647 hydrazide (can be substituted for Cy5 dye) abs: 649 nm; em: 674 nm HiLyte Fluor 680 hydrazide (can be substituted for Cy5.5) abs: 678 nm; em: 699 nm HiLyte Fluor 750 hydrazide (can be substituted for Cy7 dye) abs: 753 nm; em: 782 nm 7-Hydroxycoumarin-3-carboxylic acid abs: 387 nm; em: 448 nm Qty 5 mg 5 mg
81431
5 mg
81432 81535
81251
5 mg
1 mg
81003 81004 81502
1 mg
1 mg
81503 81006
10 mg 25 mg
81162
1 mg
81252
1 mg
81007
10 mg
81257
1 mg
81008
10 mg
81262
1 mg
81009
5 mg
81267
1 mg
81504
5 mg
81164
1 mg
81005
100 mg
81254
1 mg
81010
100 mg
81259
1 mg
81264 25 mg 25 mg 25 mg 25 mg 81253 5 mg 81258 10 mg 10 mg 81263 81268 81205 81269
1 mg
81202 81405 81505 81019
1 mg
81163
1 mg
1 mg
81020
1 mg 1 mg 1 mg 250 mg
81160 81250
81255
5 mg
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135

Fluorescent Dyes (Contd...)
Cat. No. 81206 Product 7-Hydroxycoumarin-3-carboxylic acid, succinimidyl ester abs: 363 nm; em: 447 nm 7-Hydroxy-4-methylcoumarin-3-acetic acid abs: 360 nm; em: 455 nm 7-Hydroxy-4-methylcoumarin-3acetic acid, succinimidyl ester abs: 364 nm; em: 458 nm 5-IAF, [5-Iodoacetamidofluorescein] abs: 493 nm; em: 515 nm IANBD amide, [N,N-Dimethyl-N(iodoacetyl)-N-(7-nitrobenz-2-oxa1,3-diazol-4-yl)ethylenediamine] abs: 478 nm; em: 541 nm IDCC, [7-Diethylamino-3((((2-iodoacetamido)ethyl)amino) carbonyl)coumarin] abs: 420 nm; em: 470 nm 6-JOE, SE, [6-Carboxy-4',5'-dichloro-2',7'dimethoxyfluorescein, succinimidyl ester] abs: 520 nm (pH > 9.0); em: 548 nm (pH > 9.0) LRB-EDA, [Lissamine rhodamine B ethylenediamine] abs: 560 nm; em: 581 nm LRB-SC, [Lissamine rhodamine B sulfonyl chloride] abs: (in dichloromethane): 568 nm; em: (in dichloromethane): 584 nm 7-Methoxycoumarin-3-carbonyl azide abs: 360 nm; em: 415 nm 7-Methoxycoumarin-3-carboxylic acid abs: 336 nm; em: 402 nm 7-Methoxycoumarin-3-carboxylic acid, succinimidyl ester abs: 358 nm; em: 410 nm NBD-Cl [4-Chloro-7-nitrobenzofurazan] *UltraPure Grade* abs: 337 nm; em: none NBD-F, [4-Fluoro-7-nitrobenzofurazan] abs: 337 nm; em: none NBD lysine abs: 466 nm; em: 533 NBD-X, [6-(N-(7-Nitrobenz-2-oxa-1, 3-diazol-4-yl)amino)hexanoic acid] abs: 467 nm; em: 539 NBD-X, SE, [Succinimidyl 6(N-(7-nitrobenz-2-oxa-1,3- diazol-4-yl) amino)hexanoate] abs: 466 nm; em: 535 1-Pyrenebutanoic acid abs: 341 nm; em: 376 1-Pyrenebutanoic acid, hydrazide abs: 341 nm; em: 376 1-Pyrenebutanoic acid, succinimidyl ester abs: 340 nm; em: 376 N-(1-Pyrene)maleimide abs: 338 nm; em: 375 PMIA, N-(1-Pyrenemethyl) iodoacetamide abs: 338 nm (cysteine adduct); Qty 50 mg
FLUORESCENCE
Fluorescent Dyes

Cat. No. 81244 Product 1-Pyrenesulfonyl chloride abs: 350 nm (cysteine adduct); em: 380 nm (cysteine adduct) 5(6)-ROX, [5-(and-6)-Carboxy-X-rhodamine] abs: 568 nm; em: 591nm 5-ROX, [5-Carboxy-X-rhodamine] abs: 567 nm; em: 591nm 6-ROX, [6-Carboxy-X-rhodamine] abs: 570 nm; em: 591nm 5(6)-ROX, SE, [5-(and-6)Carboxy-X-rhodamine, succinimidyl ester] abs: 576 nm; em: 601 nm 5-ROX, SE, [5-Carboxy-X-rhodamine, succinimidyl ester] abs: 573 nm; em: 602 nm 6-ROX, SE, [6-Carboxy-X-rhodamine, succinimidyl ester] abs: 575 nm; em: 602 nm SBF-Cl, [4-Chloro-7-sulfobenzofurazan, ammonium salt] abs: 380 nm; em: none SBF-F, [4-Fluoro-7-sulfobenzofurazan, ammonium salt] abs: 385 nm; em: none Sulforhodamine 101 C2 maleimide abs: 588 nm; em: 601 nm Sulforhodamine 101 cadaverine abs: 583 nm; em: 601 nm Sulforhodamine 101 hydrazide abs: 583 nm; em: 601 nm Sulforhodamine 101 lysine abs: 583 nm; em: 600 nm Sulforhodamine 101 sulfonyl chloride abs: 588 nm; em: 601 nm 5(6)-TAMRA, [5-(and-6)Carboxytetramethylrhodamine] abs: 541 nm; em: 565 nm 5-TAMRA, [5-Carboxytetramethylrhodamine] abs: 541 nm; em: 568 nm 6-TAMRA, [6-Carboxytetramethylrhodamine] abs: 541 nm; em: 568 nm 5(6)-TAMRA cadaverine, [Tetramethylrhodamine 5-(and -6)carboxamide cadaverine] abs: 544 nm; em: 570 nm 5-TAMRA cadaverine, [Tetramethylrhodamine-5-carboxamide cadaverine] abs: 545 nm; em: 576 nm 6-TAMRA cadaverine, [Tetramethylrhodamine 6-carboxamide cadaverine] abs: 544 nm; em: 575 nm 5-TAMRA Lysine, [Tetramethylrhodamine-5carboxamide lysine] abs: 545 nm; em: 577 nm 5(6)-TAMRA, SE, [5-(and-6)Carboxytetramethylrhodamine, succinimidyl ester] abs: 546 nm; em: 575 nm 5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester], abs: 547 nm; em: 574 nm Qty 100 mg
81235 81239
100 mg 25 mg
81110 81111 81112 81113
100 mg 10 mg 10 mg 25 mg
81406 81425
25 mg 25 mg
81114 25 mg 81115 81408 81409 10 mg 81447 100 mg 81510 81540 25 mg 81511 250 mg 81130 100 mg 81120 25 mg 81121 25 mg 25 mg 100 mg 81507 25 mg 81508 100 mg 100 mg 100 mg 100 mg 81125 100 mg 81509 81124 81122 81506
5 mg 5 mg 25 mg 10 mg 5 mg 5 mg 5 mg 5 mg 10 mg 25 mg
81421
81011
5 mg
81530
81108
81240 81241 81242
81203
10 mg 10 mg 10 mg
81204 81516 81212
5 mg
81213
5 mg
81236 81237 81238 81436 81435
5 mg 25 mg
5 mg
136
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Reactive Fluorescent Dyes, Fluor. Probes for Nucleic Acids

Cat. No. 81126 Product 6-TAMRA, SE, [6-Carboxytetramethylrhodamine, succinimidyl ester] abs: 547 nm; em: 573 nm 5(6)-TAMRA-X, SE, [6-(Tetramethylrhodamine-5-(and-6)-c arboxamido) hexanoic acid, succinimidyl ester] abs: 544 nm; em: 572 nm 6-TET, acid, [6-Carboxy-2',4,7,7'tetrachlorofluorescein] abs: 520 nm; em: 535 nm 6-TET, SE, [6-Carboxy-2',4,7,7'tetrachlorofluorescein, succinimidyl ester] abs: 521 nm; em: 536 nm Tetramethylrhodamine-5(and-6)-maleimide, abs: 540 nm; em: 567 nm Tetramethylrhodamine-5-maleimide abs: 540 nm; em: 567 nm Tetramethylrhodamine-6-maleimide abs: 542 nm; em: 568 nm 5-TMRIA, [Tetramethylrhodamine-5iodoacetamide] abs: 541 nm; em: 567 nm 5(6)-TRITC, [Tetramethylrhodamine-5(and-6)-isothiocyanate] abs: 543 nm; em: 571 nm 5-TRITC; G isomer, [Tetramethylrhodamine-5-isothiocyanate] abs: 543 nm; em: 571 nm 6-TRITC; R isomer , [Tetramethylrhodamine-6-isothiocyanate] abs: 544 nm; em: 572 nm Qty 5 mg
FLUORESCENCE
Fluorescent Dyes/ Probes
Fluorescent Probes for detecting Nucleic acidsCell permeant Atains

Cat. No. 83213 Product ACMA [9-Amino-6-chloro-2-methoxyacridine] abs: 412 nm; em: 471 nm Acridine orange abs: 500 nm; em: 526 nm Acridine orange, 10 mg/mL solution in water, abs: 500 nm; em: 526 nm Acridine orange 10-nonyl bromide [Nonyl acridine orange] abs: 495 nm; em: 519 nm Actinomycin D abs: 442 nm; em: none DAPI [4',6-Diamidino-2-phenylindole, dihydrochloride] abs: 358 nm; em: 461 nm DAPI [4',6-Diamidino-2-phenylindole, dihydrochloride] *Custom Packaging* abs: 358 nm; em: 461 nm Dihydroethidium [Hydroethidine] abs: 355 nm; em: N/A Dihydroethidium [Hydroethidine], 5 mM solution in DMSO abs: 355 nm; em: N/A Dihydroethidium [Hydroethidine] *Special Air-free Packaging* abs: 355 nm; em: N/A Hoechst 33258, 20 mM solution in water abs: 352 nm; em: 461 nm Hoechst 33342, 20 mM solution in water abs: 350 nm; em: N/A Hoechst 34580 abs: 392 nm; em: 440 nm Methylene Blue *UltraPure Grade* abs: 661 nm; em: N/D Methyl Green *UltraPure Grade* abs: 635 nm; em: N/D Pyronin Y *UltraPure Grade* abs: 546 nm; em: 549 nm Thiazole Orange *UltraPure Grade* abs: 512 nm; em: 533 nm Thiazole Orange, 10 mM in DMSO *UltraPure Grade* abs: 512 nm; em: 533 nm Qty 25 mg
81127
5 mg 83298 25 mg 83299 83300
1g 10 mL 25 mg
81021
81022
5 mg
81444
25 mg
83200 83210
5 mg 10 mg
81446 81445 81410
5 mg 5 mg 83211 5 mg
25 mg
81150
10 mg
85704 85718
25 mg 1 mL
81151
5 mg
81152
5 mg
85718
10 mL
83219
5 mL 5 mL 5 mg 1g 1g 100 mg 100 mg 10 mL
Fluorescent Probes for detecting Nucleic acidsCell Impermeant stains

Cat. No. 83201 83208 83209 Product 7-AAD [7-Aminoactinomycin D] abs: 546 nm; em: 647 nm EthD-1 [Ethidium homodimer-1] abs: 528 nm; em: 617 nm EthD-2 [Ethidium homodimer-2], 1 mM solution in DMSO abs: 535 nm; em: 624 nm Ethidium Bromide, 5 mM aqueous solution abs: 518 nm; em: 605 nm Ethidium Bromide *UltraPure Grade* abs: 518 nm; em: 605 nm Ethidium monoazide bromide abs: 462 nm; em: 625 nm Propidium iodide abs: 535 nm; em: 617 nm Propidium iodide, 1.0 mg/mL solution in water abs: 535 nm; em: 617 nm Stains-All abs: 573 nm; em: 609 nm Qty 1 mg
83218 83216 83239
1 mg 83238 200 ml 83237 10 x 1 mL 10 x 100 mg 5 mg 25 mg 10 ml 100 mg 83227 83228
83222 83221 83214 83212 83215 83013
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137
Labeling Nucleic acids/ FRET Probes

Amino-modified Uridine derivatives for labeling Nucleotides
Cat. No. 83202 Product Aminoallyl dUTP [5-(3-Aminoallyl)-2'-deoxyuridine 5'triphosphate, trisodium salt] Aminoallyl dUTP, 4 mM in TE buffer Aminoallyl dUTP, 4 mM in TE buffer *UltraPure Grade* Aminoallyl dUTP *UltraPure Grade* Aminoallyl dUTP *UltraPure Grade * *Custom Packaging* Aminoallyl UTP [5-(3-Aminoallyl)-uridine 5'-triphosphate, trisodium salt] ARP [N-(Aminooxyacetyl)-N-(D- biotinoyl) hydrazine, trifluoroacetic acid salt] abs: <300 nm; em: none BrdU [5-Bromo-2-deoxyuridine] abs: <300 nm; em: none BrdUTP [5-Bromo-2-deoxyuridine 5-triphosphate], 10 mM in TE Buffer abs: <300 nm; em: none BrdUTP [5-Bromouridine 5-triphosphate], 10 mM in TE Buffer abs: <300 nm; em: none Qty 1 mg
FLUORESCENE
Fluorescent Probes/ FRET Probes
DABCYL based FRET Probes

Cat. No. 81801 Product Qty
DABCYL acid, SE, [4-((4- (dimethylamino)phenyl)azo) 100 mg benzoic acid, succinimidyl ester] abs: 453 nm; em: none Appl: It is the amino-reactive form of DABCYL DABCYL C2 amine abs: 428 nm; em: none Appl: carbonyl-reactive building block for developing DABCYL-based FRET probes. DABCYL C2 maleimide abs: 428 nm; em: none Appl: thiol-reactive building block for developing DABCYL-based FRET probes. DABCYL hydrazide abs: 428 nm; em: none Appl: carbonyl-reactive building block for developing DABCYL-based FRET probes. DABCYL Plus acid abs: 437 nm; em: none It has much greater water solubility than DABCYL. DABCYL Plus acid, SE abs: 454 nm; em: none The absorption spectrum of DABCYL Plus is environment-sensitive DABCYL Plus C2 amine abs: 430 nm; em: none Appl: carbonyl-reactive building block for developing DABCYL Plus -based FRET probes. DABCYL Plus C2 maleimide abs: 430 nm; em: none Appl: thiol-reactive building block for developing DABCYL Plus based FRET probes. DABCYL Plus hydrazide abs: 430 nm; em: none Appl: carbonyl-reactive building block for developing DABCYL Plus -based FRET probes. DABSYL-L-alanine abs: 436 nm; em: none Appl: reference standard for the HPLC analysis of L-alanine and alanine-containing peptides. DABSYL chloride *UltraPure Grade* [4-dimethylaminoazobenzene-4'- sulfonyl chloride] abs: 436 nm (butylamine adduct); em: none Appl: acceptor for developing FRET-based nucleic acid probes and protease substrates DABSYL-glycine abs: 436 nm; em: none Appl: standard for HPLC analysis of glycine and glycin-containing peptides. 100 mg
83206 83203 83204 83205 83207
250 ml 250 ml 1 mg 1 mg 1 mg
81819
81802
25 mg
81820
100 mg
60645
10 mg 81803 25 mg 81804 100 uL 81823 100 uL 81805
100 mg
83224 83225
25 mg
10 mg
83226
10 mg
HiLyte Fluor Dyes for Labelling Nucleic Acids

81824 Cat. No. 71238 Product HiLyte Fluor Amine-Reactive Dye, HiLyte Fluor 488, SE *5-Pack* HiLyte Fluor Amine-Reactive Dye, HiLyte Fluor 555, SE *5-Pack* HiLyte Fluor Amine-Reactive Dye, HiLyte Fluor 647, SE *5-Pack* Qty 0.1 mg 81808 0.1 mg
10 mg
25 mg
71239
71240
0.1 mg
81806
1g

Cat. No. 23490 Product t-BOC-Lys(DABCYL)-OH abs: 428 nm; em: none Appl: A useful building block for FRET probes containing DABCYL (as acceptor) DABCYL acid, [4-((4-(dimethylamino) phenyl)azo) benzoic acid] *UltraPure Grade* abs: 425 nm; em: none Qty 100 mg 81807 81809
25 mg
DABSYL hydrazine abs: 436 nm; em: none 100 mg Appl: building block that can be coupled to an aldehyde (for carbohydrates or glycoproteins) or carboxy group (for peptides or proteins).
81800
1g
138
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FRET Probes and Quenchers

Cat. No. 81810 Product DABSYL-L-leucine abs: 436 nm; em: none reference standard for HPLC analysis of L-leucine and leucinecontaining peptides. DABSYL-L-methionine abs: 436 nm; em: none reference standard for HPLC analysis of L-methionine and methionine-containing peptides. DABSYL-L-proline abs: 436 nm; em: none reference standard for HPLC analysis of L-proline and proline-containing peptides. DABSYL-L-tryptophan abs: 436 nm; em: none reference standard for HPLC analysis of L-tryptophan and tryptophancontaining peptides. DABSYL-L-valine abs: 436 nm; em: none reference standard for HPLC analysis of L-valine and valinecontaining peptides. FMOC-Lys(DABCYL)-OH, abs: 428 nm; em: none building block for FRET probes containing DABCYL (as acceptor). FMOC-Lys(DABCYL) AnaResin abs: 428 nm; em: none building block for FRET probes containing DABCYL (as acceptor) in solid-phase synthesis. Qty 25 mg
FLUORESCENCE
FRET Probes/Quenchers
Quenchers
DNP: An excellent amine-reactive FRET quencher paired with Trp or Tyr Cat. No. 81227 Product DNP-X acid, [6-(2,4-Dinitrophenyl) aminohexanoic acid] abs: ~350 nm; em: none DNP-X acid, SE, [6-(2,4-Dinitrophenyl) aminohexanoic acid, succinimidyl ester] abs: ~350 nm; em: none DNP C2 amine abs: ~350 nm; em: none DNP C2 maleimide abs: ~350 nm; em: none Qty 100 mg
81811
25 mg
81228
25 mg
81821 25 mg 81822
100 mg
81812
25 mg
81813
25 mg
QXL:
QXL 490 dyes are the optimized quenchers for EDANS, AMCA and most coumarin fluorophores. QXL 490 acid can be coupled to amino groups via EDC-mediated reactions.
81814
25 mg
QXL 520 dyes are optimized quenchers for fluoresceins such as FAM, FITC, Rhodamine 6G and HiLyte Flour 488 flurophores. Cat. No. 81825 Product QXL 490 acid abs: 485 nm (in water); em: none QXL 490 acid, SE abs: 495 nm(in water); em: none readily reacts with amino-containing compounds. QXL 490 C2 amine abs: 485 nm(in water); em: none readily reacts with carbonyl-containing compounds. QXL 490 C2 maleimide abs: 485 nm(in water); em: none readily reacts with thiol-containing compounds. QXL 490 hydrazide abs: 485 nm(in water); em: none readily reacts with carbonyl-containing compounds. QXL 520 acid abs: 508 and 530 nm; em: none can be coupled to amino groups via EDC-mediated reactions. QXL 520 acid, SE abs: 508 and 530 nm; em: none reacts with amino-containing compounds. QXL 520 C2 amine abs: 508 and 530 nm; em: none readily reacts with carbonyl-containing compounds. QXL 520 C2 maleimide abs: 508 and 530 nm; em: none readily reacts with thiol-containing compounds. QXL 520 hydrazide abs: 508 and 530 nm; em: none readily reacts with carbonylcontaining compounds. Qty 100 mg 25 mg
23496
100 mg 81826
23858
500 mg 81828
10 mg
EDANS based FRET Probes

Cat. No. 23486 Product t-BOC-Asp(EDANS)-OH abs: 341 nm; em: 470 building block for FRET probes containing EDANS t-BOC-Glu(EDANS)-OH abs: 341 nm; em: 470 building block for FRET probes containing EDANS EDANS acid, [5-((2-aminoethyl)amino) naphthalene-1-sulfonic acid] abs: 335 nm; em: 493 EDANS, sodium salt, [5-((2aminoethyl) amino)naphthalene-1-sulfonic acid, sodium salt] abs: 335 nm; em: 493 used for preparing FRET-based nucleic acid probes and protease substrates. FMOC-Asp(EDANS)-OH abs: 341 nm; em: 470 building block for FRET probes containing EDANS FMOC-Glu(EDANS)-OH abs: 341 nm; em: 470 building block for FRET probes containing EDANS Qty 100 mg
81827
10 mg
81846
5 mg
23488
100 mg
81830
100 mg
23887
1g
81831
25 mg
81535
81832 1g
10 mg
23492
100 mg
81833
10 mg
23494
100 mg
81847
5 mg
! +91-40-27017477 / 65916167 Fax : +91-40-27016168
139
FRET Probes & Quenchers

QXL (Contd.)
QXL 570 dyes are optimized quenchers for rhodamines (such as TAMRA, sulforhodamine B, ROX) and Cy3 flurophores. It can be coupled to amino groups via EDC-mediated reaction. QXL 610 dyes are optimized quenchers for ROX and Texas Red fluorophores. QXL 610 acid can be coupled to amino groups via EDCmediated reactions. QXL 670 dyes are optimized quenchers for Cy5 and Cy5-like fluorophores such as HiLyte Fluor 647. QXL 670 acid can be coupled to amino groups via EDC-mediated reactions. Cat. No. 81835 81836 Product QXL 570 acid abs: 577 nm; em: none QXL 570 acid, SE abs: 578 nm; em: none readily reacts with amino-containing compounds. QXL 570 C2 amine abs: 577 nm; em: none readily reacts with carbonyl-containing compounds. QXL 570 C2 maleimide abs: 577 nm; em: none readily reacts with thiolcontaining compounds. QXL 570 hydrazide abs: 578 nm; em: none readily reacts with carbonyl-containing compounds. QXL 610 acid abs: 594 nm and 628 nm; em: none QXL 610 acid, SE abs: 594 nm and 628 nm; em: none readily reacts with aminocontaining compounds. QXL 610 C2 amine abs: 594 nm and 628 nm; em: none readily reacts with carbonyl-containing compounds. QXL 610 hydrazide abs: 594 nm and 628 nm; em: none readily reacts with carbonyl-containing compounds. QXL 610 vinyl sulfone *Thiol-Reactive* abs: 594 nm and 628 nm; em: none readily reacts with thiolcontaining compounds. QXL 670 acid abs: 668 nm; em: none QXL 670 acid, SE abs: 668 nm; em: none readily reacts with aminocontaining compounds. QXL 670 C2 amine abs: 668 nm; em: none readily reacts with carbonyl-containing compounds. QXL 670 C2 maleimide abs: 668 nm; em: none readily reacts with thiol-containing compounds. QXL 670 hydrazide abs: 668 nm; em: none readily reacts with carbonyl-containing compounds. Qty 25 mg 10 mg 81852
FLUORESCENCE
Fluorescent Probes/ FRET Probes
QXL (Contd.)
QXL 680 dyes are optimized quenchers for Cy5 and Cy5-like fluorophores such as HiLyte Fluor 647. QXL 680 acid reacts with amine and hydroxylcontaining compounds through EDC-mediated reactions. Cat. No. 81855 81851 Product QXL 680 acid abs: 679 nm; em: none QXL 680 acid, SE abs: 679 nm; em: none readily reacts with amino-containing compounds. QXL 680 C2 amine abs: 679 nm; em: none readily reacts with carbonyl-containing compounds. QXL 680 C2 maleimide abs: 679 nm; em: none readily reacts with thiol-containing compounds. QXL 680 hydrazide abs: 679 nm; em: none readily reacts with carbonyl-containing compounds. Qty 10 mg 5 mg
1 mg
81854
1 mg
81837
5 mg
81853
1 mg
81838
5 mg
81848
5 mg
81815 81816
100 mg 25 mg
81818
25 mg
81849
10 mg
81817
25 mg
81840 81841
10 mg 5 mg
81842
5 mg
81843
5 mg
81850
5 mg
140
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Quantification & Detection of Proteins on Gels/Solution

AnaLyte OPA Protein Quantitation Kit *Fluorimetric*
AnaLyte OPA Protein Quantitation Kit is designed to quantify lower concentrations of proteins. This kit that employs the OPA reagent for rapid and sensitive protein quantitation in solution. The kit functions well in the presence of lipids and detergents, the substances that interfere with many other protein determination methods.The kit is best used to determine proteins in the range from 50 ng/mL to 25 mg/mL. Cat. No. 71015 Product AnaLyte OPA Protein Quantitation Kit *Fluorimetric* Qty 500 assays 81529
FLUORESCENCE
Protein detection
Detecting Proteins on Gels

Cat. No. 83004 83005 81513 Product Alcian Blue 8GX *UltraPure Grade* abs: 615 nm; em: N/D nm Alcian Blue 8GX Plus*UltraPure Grade* abs: 670 nm; em: N/D nm 2-Aminoacridone abs: 425 nm; em: 531 nm ANDS [7-Aminonaphthalene-1,3disulfonic acid, potassium salt] abs: 350 nm; em: 450 nm 2,6-ANS [2-Anilinonaphthalene6-sulfonic acid] abs: 319 nm; em: 422 nm 1,8-ANS [1-Anilinonaphthalene8-sulfonic acid] abs: 319 nm; em: 422 nm ANTS [8-Aminonaphthalene-1,3, 6-trisulfonic acid, disodium salt] *UltraPure Grade* abs: 353 nm; em: 520 nm APTS [8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt] abs: 424 nm; em: 505 nm Biotin hydrazide Biotin-XX hydrazide [6-((6-((Biotinoyl)amino)hexanoyl)amino) hexanoic acid, hydrazide] Congo Red *UltraPure Grade* abs: 497 nm; em: N/A nm Coomassie Brilliant Blue G-250 *UltraPure Grade* abs: 610 nm; em: N/A nm Coomassie Brilliant Blue R-250 *UltraPure Grade* abs: 585 nm; em: N/A nm DABSYL hydrazide abs: 436 nm; em: N/A nm Dansyl hydrazide [5-Dimethylaminonaphthalene-1sulfonyl hydrazine] abs: 336 nm; em: 534 nm Eosin Y *UltraPure Grade* Abs(max): 517 nm; em: N/D nm Fast Green FCF *UltraPure Grade* abs: 622 nm; em: N/A nm 5-FTSC [Fluorescein-5-thiosemicarbazide] abs: 492 nm; em: 516 nm Nile Red abs: 552 nm; em: 636 nm 10% Ninhydrin in ethanol abs: N/D nm; em: N/D nm Oil Red O *UltraPure Grade* abs: 518 nm; em: N/A nm Ponceau S *UltraPure Grade* abs: 520 nm; em: N/A nm Stains-All abs: 573 nm; em: 609 nm Qty 100 mg 100 mg 25 mg 1g
Reagents Used for Quantifying Peptides and Proteins in Solution

Cat. No. 81448 Product bBBr *UltraPure Grade* [Dibromobimane] abs: 393 nm; em: 490 nm DABSYL hydrazide abs: 436 nm; em: N/A nm Dansyl hydrazide [5-Dimethylaminonaphthalene-1sulfonyl hydrazine] abs: 336 nm; em: 534 nm Fluorescamine *UltraPure Grade* abs: 315 nm; em: None mBBr *UltraPure Grade* [Monobromobimane] abs: 395 nm; Em(max): 490 nm NBD-Cl *UltraPure Grade* [4-Chloro-7-nitrobenzofurazan] abs: 337 nm; em: None NBD-F *UltraPure Grade* [4-Fluoro-7-nitrobenzofurazan] abs: 337 nm; em: None NDA *UltraPure Grade* [Naphthalene-2,3-dicarboxaldehyde] abs: 4194 nm; em: 4934 nm OPA *UltraPure Grade* [o-Phthaldialdehyde] abs: 3345 nm; em: 4565 nm SBF-Cl [4-Chloro-7-sulfobenzofurazan, ammonium salt] abs: 380 nm; em: None SBF-F [4-Fluoro-7-sulfobenzofurazan, ammonium salt] abs: 385 nm; em: None Qty 25 mg
81524
100 mg
81525
100 mg
81528
100 mg
81807
100 mg 81523 100 mg 60650 60652 25 mg 83016 25 mg 83002
10 mg
81520
25 mg 25 mg
81202
1g 100 mg
81433
81203
100 mg
83003
1g
81204
25 mg
81807 81520
100 mg 100 mg
83010
100 mg
83012
1g
83017 83007
1g 1g 25 mg 25 mg 10 ml 1g 1g 100 mg
81408
25 mg
81505 83011
81409
10 mg
83000 83008 83009 83013
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141
Kits for Labeling Proteins with dyes & enzymes

AnaTag Protein Labeling Kits AnaTag AMCA-X Protein Labeling Kit
This kit is optimized to conjugate AMCA-X to proteins (e.g., IgG). One conjugation reaction can label up to 5 mg protein.
FLUORESCENCE
Labeling & Detection of Proteins
AnaTag APC Protein Labeling Kit

The EnzoLyte APC protein labeling kit is optimized for labeling APC-XL (a stable, chemically cross-linked APC trimer) to antibodies and other proteins. APC (Allophycocyanin) is an ultra-sensitive fluorescent tracer because of its high emission quantum yields. Its fluorescence can be detected at the emission wavelength of 6605 nm or at 6505 nm when excited. Cat. No. 71011 Product AnaTag APC Protein Labeling Kit Qty 1 kit
AnaTag 5-FAM Protein Labeling Kit

This kit is optimized to conjugate 5-FAM (5-Carboxyfluorescein) to proteins (e.g., IgG). One conjugation reaction can label up to 5 mg protein.
AnaTag 5-TAMRA Protein Labeling Kit

This kit is optimized to conjugate 5-TAMRA (5Carboxytetramethylrhodamine) to proteins (e.g., IgG). One conjugation reaction can label up to 5 mg protein.
AnaTag BPE Protein Labeling Kit

The EnzoLyte BPE protein labeling kit is optimized for labeling BPE to antibodies and other proteins. B-PE (B-Phycoerythrin) is a fluorescent protein that has absorption bands with peaks at 545 nm and 563 nm and has maximal emission at 578 nm. B-PE labeled biotin, avidin, and primary and secondary antibodies have been widely applied to flow cytometry, live cell staining, and multi-color immunofluorescent staining. Cat. No. 71012 Product AnaTag BPE Protein Labeling Kit Qty 1 kit
AnaTag 5-ROX Protein Labeling Kit

This kit is optimized to conjugate 5-ROX (5-Carboxy-X-rhodamine) to proteins (e.g., IgG). One conjugation reaction can label up to 5 mg protein.
AnaTag TR Protein Labeling Kit

This kit is optimized to conjugate HiLyte Fluor TR to proteins (e.g., IgG). One conjugation reaction can label up to 1 mg protein.
AnaTag HiLyte Fluor 555 Protein Labeling Kit

This kit is optimized to conjugate HiLyte Fluor 555 SE to proteins (e.g., IgG). One conjugation reaction can label 100 g of protein.
AnaTag RPE Protein Labeling Kit

The EnzoLyte RPE protein-labeling kit is optimized for labeling RPE to antibodies and other proteins. R-PE (R-Phycoerythrin), a fluorescent protein, belongs to the phycobiliproteins family. It has broad absorption bands with peaks at 565 nm, 498 nm, and 539 nm, therefore can be excited with versatile excitation sources. Its maximal emission can be detected at 578 nm. R-PE labeled biotin, avidin, primary and secondary antibody have been widely applied to flow cytometry, live cell staining, and multi-color immunofluorescent staining.
AnaTag HiLyte Fluor 647 Protein Labeling Kit

This kit is optimized to conjugate HiLyte Fluor 647 SE to proteins (e.g., IgG). One conjugation reaction can label 100 g of protein.
AnaTag Biotin Protein Labeling Kit

This kit is optimized to conjugate biotin-X, SE (d-Biotin-amidocaproateN-hydroxysuccinimide ester) to proteins (e.g., IgG). One reaction can label up to 10 mg IgG.
Cat. No. 71013
Product AnaTag RPE Protein Labeling Kit 1 Reaction (1x1 mg protein)
Qty 1 kit
AnaTag AP Protein Labeling Kit

The AnaTagTM Alkaline Phosphatase Protein Labeling Kit is optimized for labeling proteins with alkaline phosphatase. This kit provides ample materials to label and desalt up to 1 mg of Ig G.
AnaTag HRP Protein Labeling Kit

This kit provides an ultra convenient method to conjugate HRP to protein. It contains formulated and preactivated HRP, stabilizer and all necessary reagents/buffer to prepare one reaction of HRP-protein conjugation. Cat. No. 71000 71001 71002 71005 71006 71007 71008 71003 71009 71010 Product AnaTag AMCA-X Protein Labeling Kit AnaTag 5-FAM Protein Labeling Kit AnaTag 5-TAMRA Protein Labeling Kit AnaTag 5-ROX Protein Labeling Kit AnaTag TR Protein Labeling Kit AnaTag HiLyte Fluor 555 Protein Labeling Kit AnaTag HiLyte Fluor 647 Protein Labeling Kit AnaTag Biotin Protein Labeling Kit AnaTag AP Protein Labeling Kit AnaTag HRP Protein Labeling Kit Qty 5 Rxns. 5 Rxns. 5 Rxns. 5 Rxns. 5 Rxns. 5 Rxns. 5 Rxns. 5 Rxns. 1 kit 1 kit
AnaLyte CB Protein Quantitation Kit *Colorimetric*

AnaLyte CB Protein Quantitation Kit is a modification of Bradford method. This kit is designed to quantify concentrations of proteins higher than 1,500 g/mL. Sufficient reagents are provided to perform 1000 assays, based on a 200 L assay volume in a 96-well microplate format. The assay can also be adapted for use in cuvettes. The assay is based on the immediate absorption shift from 465 nm to 595 nm when Coomassie Blue G-250 binds to proteins in acidic solutions. The kit has been specially formulated with ready-to-use buffers, prediluted standards. Common contaminants, including salts, solvents, mercaptoethanol, amino acids and DNA, are well tolerated in this assay. However, it is not compatible with detergents. Cat. No. 71014 Product AnaLyte CB Protein Quantitation Kit *Colorimetric* Qty 500 assays
142
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Calcium Detection Reagents

UV- Excitable Calcium Indicators Fura-2
abs : 363 (335) nm; em: 512 (505) nm Cat. No. 84015 84016 84017 84018 84019 84011 84012 Product Fura-2, AM Fura-2, AM *UltraPure Grade* Fura-2, AM *UltraPure Grade* Custom Packaging Fura-2, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO Fura-2, AM *UltraPure Grade* Bulk Packaging Fura-2, pentapotassium salt Fura-2, pentasodium salt Qty 1 mg 1 mg 1 mg 1 ml 50 mg 1 mg 1 mg
FLUORESCENCE
Calcium Detection
Dihydrocalcein
abs : 494 nm; em: 517 nm Reduced form of calcein AM Cat. No. 85700 85701 Product Dihydrocalcein, AM Dihydrocalcein, AM *Custom Packaging* Qty 1 mg 1 mg
Fluo-3
abs : 506 nm; em: 526 nm The most popular cell-permeable calcium indicator for functional GPCR assays Cat. No. 84024 84025 84026 84027 Product Fluo-3, AM Fluo-3, AM *UltraPure Grade* Fluo-3, AM *UltraPure Grade* Custom Packaging Fluo-3, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO Fluo-3, AM *UltraPure Grade* *Bulk Package* Fluorescence enhancement upon binding Ca2+, Mg2+, Ba2+, Cd2+, Hg2+, Pb2+, Zn2+ and La3+ Fluo-3, pentaammonium salt Fluo-3, pentapotassium salt Fluo-3, pentasodium salt Qty 1 mg 1 mg 1 mg 1 mL 50 mg
Indo-1
Cat. No. 84006 84007 84008 84009 84010 84000 84001
abs : 346 (330) nm; em: 475 (401) nm Product Indo-1, AM Indo-1, AM *UltraPure Grade* Indo-1, AM *UltraPure Grade* Custom packaging Indo-1, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO Indo-1, AM *UltraPure Grade* *Bulk packaging* Indo-1, pentapotassium salt Indo-1, pentasodium salt Qty 1 mg 1 mg 1 mg 1 mg 50 mg 1 mg 1 mg
84028
84020 84021 84022
1 mg 1 mg 1 mg
Fluo-5F
abs : 494 nm; em: 518 nm Cat. No. 84055 Product Fluo-5F, pentapotassium salt Sensitive fluorescent Ca2+ indicator, Kd = 2.3 mM Fluo-5F, AM Cell-permeable fluorescent Ca2+ indicator Qty 1 mg
Indo-5F
Cat. No. 84050 84051
abs : 347 (331) nm; em: 475 (412) nm Product Indo-5F, pentapotassium salt Indo-5F, AM Qty 1 mg 1 mg
84056
1 mg
Fluo-5N
abs : 593 nm; em: 518 nm Cat. No. 84057 Product Fluo-5N, pentapotassium salt Fluorescence enhancement upon binding Ca2+, Cd2+, Hg2+ and La3+ Fluo-5N, AM Low affinity cell-permeable Ca2+ indicator Qty 1 mg
Visible Light-Excitable Calcium Indicators Calcein

abs : 494 nm; em: 517 nm Cat. No. 89200 89201 89202 89203 89204 89206 89205 Product Calcein *UltraPure Grade* Calcein, AM Calcein, AM *UltraPure Grade* Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO Calcein, AM *UltraPure Grade* *Custom Packaging* Calcein blue Abs (max): 322 nm; Em (max): 437 nm Calcein blue, AM Abs (max): 494 nm; Em (max): 517 nm Qty 100 mg 1 mg 1 mg 200 L 1 mg 250 mg 25 mg
84058
1 mg
Visible Light-Excitable Calcium Indicators X-Rhod-1

abs : 580 nm; em: 602 nm Cat. No. 84049 84048 Product X-Rhod-1, tripotassium salt X-Rhod-1, AM Qty 1 mg 1 mg
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143
Calcium Detection Reagents

Rhod-2
abs : 549 nm; em: 578 nm Long wavelength fluorescent indicator for quantifying intracellular Ca2+ concentration Cat. No. 84034 84035 84036 84037 84038 84030 Product Rhod-2, AM Rhod-2, AM *UltraPure Grade* Rhod-2, AM *UltraPure Grade* Custom Pack Rhod-2, AM *UltraPure Grade*, 1 mM solution in anhydrous DMSO Rhod-2, AM *UltraPure Grade* Bulk Pack Rhod-2, tripotassium salt Fluorescence enhancement upon binding Ca2+, Mg2+, Ba2+, Cd2+, Hg2+, Pb2+, Zn2+ and La3+ Rhod-2, trisodium salt Fluorescence enhancement upon binding Ca2+, Mg2+, Ba2+, Cd2+, Hg2+, Pb2+, Zn2+ and La3+ Qty 1 mg 1 mg 1 mg 1 mg 50 mg 82258 82259 82260 82256 82257 Cat. No. 82255 Product
FLUORESCENCE
Calcium Detection
Qty 0.25 mg 0.25 mg 0.25 mg 0.25 mg 0.25 mg 0.25 mg
Coelenterazine *UltraPure Grade* Abs (max): 429 nm; Em (max): 466 nm Coelenterazine cp *UltraPure Grade* Abs (max): 430 nm; Em (max): 442 nm Coelenterazine f *UltraPure Grade* Abs (max): 437 nm; Em (max): 472 nm Coelenterazine h *UltraPure Grade* Abs (max): 437 nm; Em (max): 466 nm Coelenterazine hcp *UltraPure Grade* Abs (max): 437 nm; Em (max): 445 nm Coelenterazine n *UltraPure Grade* Abs (max): 431 nm; Em (max): 468 nm
1 mg
Non- Luminescent Calcium Indicators

Cat. No. Product BAPTA, AM # BAPTA, AM *UltraPure Grade* # BAPTA, tetrapotassium salt ## BAPTA, tetrasodium salt ## 5,5-Difluoro BAPTA, AM # 5,5-Difluoro BAPTA, tetrapotassium salt ## 5,5-Dimethyl BAPTA, AM # 5,5-Dimethyl BAPTA, tetrapotassium salt ## 5,5-Dinitro BAPTA, AM # 5,5-Dinitro BAPTA, free acid ## EGTA, AM # EGTA, tetrasodium salt, 10 mM aqueous solution *UltraPure Grade* A chelating agent for the determination of calcium in the presence of magnesium Pluronic F-127 *Cell Culture Tested * Cell culture reagent for dissolving AM esters Pluronic F-127, 20% solution in DMSO Cell culture reagent for dissolving AM esters Pluronic F-127, 10% solution in water Cell culture reagent for dissolving AM esters Qty 25 mg 25 mg 100 mg 100 mg 25 mg 100 mg 25 mg 100 mg 25 mg 100 mg 10 mg 10 mL
84031
1 mg
84070 84071 84072 84073
Rhod-5F
abs : 580 nm; em: 602 nm Long wavelength fluorescent Ca2+ indicator Cat. No. 84059 84060 Product Rhod-5F, tripotassium salt Rhod-5F, AM Qty 1 mg 1 mg
84074 84075 84076 84077
Rhod-5N
abs : 551 nm; em: 577 nm Cat. No. 84061 84062 Product Rhod-5N, tripotassium salt Rhod-5N, AM Qty 1 mg 1 mg
84080 84081 84100 84097
Coelenterazine Sampler Kit *UltraPure Grade*

This Coelenterazine Sampler Kit consists of coelenterazine and four of its derivatives, 25 g each, for reconstituting aequorin in cells that have been transfected with apoaequorin DNA. The coelenterazine analogs confer different Ca2+ affinities and spectral properties on he aequorin complex. Cat. No. 71403 Product Qty
84040 84041 84042
10 g 10 mL 100 mL
Coelenterazine Sampler Kit *UltraPure Grade* 1 kit
# Cell permeable ## Cell impermeable
Coelenterazine and Its Synthetic Analogs for Luminescent Calcium Detection

The aequorin complex comprises a 22,000-dalton apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. It has a broad detection range from ~0.1 M to >100 M. Aequorins containing the cp, f or h form of coelenterazine exhibit 1020 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazine cp has been used in HTS screening assay for GPCRs.
144
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Fluorecent substrates for Esterase/Oxidase for Cell Viability

Fluorogenic Esterase and Oxidase Substrates Used for Cell Viability Assays
Cat. No. 84602 Product BCECF, AM, 1 mg/mL solution in anhydrous DMSO abs: 503 nm; em : 520 nm, BCECF, AM *Custom Packaging* Properties same as above 5-(and-6)-Carboxy-2',7'dichlorodihydrofluorescein diacetate abs: 495 nm; em : 529 nm) Cell-impermeable substrate for detection of oxidases (including peroxidase) 5-Carboxy-2',7'-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) abs: 495 nm; em : 529 nm Cell-permeable substrate for fluorogenic detection of oxidases (including peroxidase) 5-(and-6)-Carboxy-2',7'dichlorofluorescein diacetate *Mixed Isomers* abs: 495 nm; em : 529 nm pH indicator for acidic pH range 5(6)-CFDA [5-(and-6)-Carboxyfluorescein diacetate] *Mixed Isomers* abs: 492 nm; em : 517 nm, pH indicator for slightly acidic pH range 5-CFDA [5-Carboxyfluorescein diacetate] *Single Isomers* abs : 492 nm; em : 517 nm) pH indicator for slightly acidic pH range 6-CFDA [6-Carboxyfluorescein diacetate] *Single Isomers* abs: 492 nm; em : 517 nm) pH indicator for slightly acidic pH range 5-CFDA, AM [5-Carboxyfluorescein diacetate, acetoxymethyl ester] abs : 492 nm; em : 517 nm Cell-permeable pH indicator for slightly acidic pH range 2',7'-Dichlorodihydrofluorescein diacetate [2',7'-Dichlorofluorescin diacetate] abs: 495 nm; em : 529 nm Cell-permeable substrate for detection of oxidases (including peroxidase) Dihydrocalcein, AM abs: 494 nm; em : 517 nm Generic cell permeable substrate for detection of oxidases (including peroxidase); is well retained in cells. Dihydrocalcein, AM *Custom Packaging* abs: 494 nm; em : 517 nm Qty 1 ml
FLUORESCENCE
Cell viability, proliferation & toxicity
Fluorogenic Esterase and Oxidase Substrates Used for Cell Viability Assays (Contd.)
Cat. No. 85701 Product Dihydrocalcein, AM abs: 494 nm; em 517 nm * Reduced form of calcein AM Dihydroethidium *Special Air-free Packaging* abs: 518 nm; em: 605 nm Generic substrate for detection of oxidases (including peroxidase); The oxidized product tends to accumulate in nuclei. Dihydroethidium 5 mM solution in DMSO abs: 355 nm; em: N/A Bind to DNA/RNA (red fluorescence) upon oxidation Dihydrofluorescein diacetate abs: 492 nm; em: 517 nm Generic substrate for detection of oxidases (including peroxidase) Dihydrorhodamine 123 abs : 507 nm; em: 529 nm Generic substrate for detection of oxidases (including peroxidase) in mitochondria Dihydrorhodamine 123 *Air-free Packaging* Properties same as above Dihydrorhodamine 123, 5 mM solution in DMSO Properties same as above Dihydrorhodamine 6G abs: 528 nm; em: 551 nm Generic substrate for detection of oxidases (including peroxidase) in mitochondria FDA [Fluorescein diacetate] abs: 490 nm; em: 514* nm, Cell-permeable pH indicator for slightly acidic pH range Qty 1 mg
84603
1 mg
85717
10 mg
85703
25 mg
85718 5 mg 85710
1 mL/ 10 mL
85702
25 mg
89003
100 mg 85711 10 mg
89005
100 mg
85720 85719
10 mg 1 mL 25 mg
89007
100 mg
85712
89008
100 mg
88400
1g
89009
5 mg
Fluorescent Vital Stains for Monitoring Cellular Functions

Cat. No. Product DBDS [4,4'-Dibenzamidostilbene-2,2'disulfonic acid, disodium salt] abs : 343 nm; em: 430 nm Environment-sensitive dye for studying membrane anion transporter DNDS [4,4'-Dinitrostilbene-2,2'disulfonic acid, disodium salt] abs : 352 nm; em: N/A Fluorescent probe for studying ion transporter DiA [4-(4-(Dihexadecylamino) styryl)-Nmethylpyridinium iodide] abs : 491 nm; em: 613 nm Fluorescent lipophilic tracer Qty 100 mg
85706
100 mg
84737
85700
1 mg
84915
1g
84710 85701 1 mg
25 mg
Also available Calcein & Calcein Blue. Please view page #
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145
Fluorescent Stains for Cell Viability

Fluorescent Vital Stains for Monitoring Cellular Functions (Contd.)
Cat. No. 84700 Product DiBAC4(3) *UltraPure Grade* [Bis-(1,3-dibutylbarbituric acid) trimethine oxonol] abs: 493 nm; em: 516 nm Sensitive 488 nm-excitable membrane potential probe, ~1% fluorescence intensity change/mV; DiBAC4(5) *UltraPure Grade* [Bis-(1,3-dibutylbarbituric acid) pentamethine oxonol] abs: 590 nm; em: 616 nmv Sensitive membrane potential probe with longer wavelength DiD [1,1'-Dioctadecyl-3,3,3',3' tetramethylindodicarbocyanine perchlorate] *Oil* abs: 644 nm; em: 665 nm Lipophilic membrane tracer DiD [1,1'-Dioctadecyl-3,3,3',3' tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt] *Solid* Properties same as above DIDS [4,4'-Diisothiocyanatostilbene-2,2'disulfonic acid, disodium salt] abs: 341 nm; em: 415 nm Reactive environment-sensitive dye for studying receptors and proteins DiI [1,1'-Dioctadecyl-3,3,3',3'tetramethylindocarbocyanine perchlorate] abs: 549 nm; em: 565 nm A lipophilic membrane stain that diffuses laterally to stain the entire cell; Its D9-DiI [1,1'-Dioleyl-3,3,3',3'tetramethylindocarbocyanine methanesulfonate] abs: 549 nm; em: 564 nm A lipophilic membrane stain DiIC1(5) [1,1',3,3,3',3'Hexamethylindodicarbocyanine iodide] abs: 638 nm; em: 658 nm Used for measurement of mitochondrial membrane potential DiIC12(3) [1,1'-Didodecyl-3,3,3',3'tetramethylindocarbocyanine perchlorate] abs: 549 nm; em: 565 nm Lipophilic neuronal tracer DiIC16(3) [1,1 abs: 549 nm; em: 565 nm Lipophilic neuronal tracerss DiIC18(3)-DS [1,1'-Dioctadecyl-3,3,3',3' tetramethylindocarbocyanine-5,5' -disulfonic acid] abs: 555 nm; em: 570 nm Lipophilic neuronal tracer Qty 25 mg
FLUORESCENCE

Cat. No. 84908 Product DiIC18(5)-DS [1,1'-Dioctadecyl-3,3,3',3' tetramethylindodicarbocyanine-5,5'disulfonic acid] abs: 650 nm; em: 670 nm Lipophilic neuronal tracer DiO [3,3'-Dioctadecyloxacarbocyanine perchlorate abs: 484 nm; em: 501 nm Lipophilic neuronal tracer DiOC2(3) [3,3'-Diethyloxacarbocyanine iodide] abs: 482 nm; em: 497 nm Membrane potential-dependent red-shifted fluorescence DiOC5(3) [3,3'-Dipentyloxacarbocyanine iodide] abs: 484 nm; em: 500 nm measuring membrane potential DiOC6(3) [3,3'-Dihexyloxacarbocyanine iodide] abs: 484 nm; em: 501 nm Most widely used for measuring membrane potential DiOC7(3) [3,3'-Diheptyloxacarbocyanine iodide] abs: 482 nm; em: 504 nm Used for measuring membrane potential of mitochondria DiOC16(3) [3,3'-Dihexadecyloxacarbocyanine perchlorate] abs: 484 nm; em: 501 nm Lipophilic membrane tracer 1,1'-Dioctadecyl-5,5'-diphenyl-3,3,3', 3' tetramethylindocarbocyanine chloride abs: 576 nm; em: 599 nm Lipophilic neuronal tracer DiR [1,1'-Dioctadecyl-3,3,3',3' tetramethylindotricarbocyanine iodidev abs: 748 nm; em:780 nmv Lipophilic neuronal tracer DiSBAC2(3) [Bis-(1,3-diethylthiobarbituric acid) trimethine oxonol] abs: 535 nm; em: 560 nm Sensitive membrane potential probe, less temperature-dependent than DiBAC DiSC3(5) [3,3'-Dipropylthiadicarbocyanine iodide] abs: 651 nm; em: 675 nm Accumulate in cells on hyperpolarized membranes 5-Dodecanoylaminofluorescein abs: 495 nm; em: 518 nm Cell membrane stain DPH [1,6-Diphenyl-1,3,5-hexatriene] abs:350 nm; em: 452 nm Environment-sensitive dye for studying membrane and protein structures Qty 5 mg
84712 84701 25 mg
100 mg
84706
100 mg
84903
25 mg 84714 100 mg
84715 84920 10 mg
100 mg
84707 84904 100 mg
100 mg
84708 100 mg
25 mg
84711
84916
5 mg
84906
25 mg 84922
10 mg
84709
100 mg 84702 25 mg/ 100 mg
84902
100 mg 84923 100 mg
84905
100 mg 88200 100 mg
84907
5 mg 88202 25 mg
146
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Fluorecent Stains for Cell Viability/Nucleic acid stains

Cat. No. 88203 Product DPH propionic acid [3-(4-(6-Phenyl)-1,3,5-hexatrienyl) phenylpropionic acid] abs: 354 nm; em: 430 nm Cell membrane stain JC-1 [5,5',6,6'-tetrachloro-1,1',3,3'tetraethylbenzimidazolylcarbocyanine iodide] abs: 514 nm; em: 529 nm Widely used for measuring membrane potential of mitochondria; 585/520 Fluorescence ratio increases upon cell hyper-polarization. Laurdan [6-Dodecanoyl-2-dimethylaminonaphthalene] abs: 364 nm; em: 497 nm Environment-sensitive dye for studying membrane and protein structures Merocyanine 540 abs: 555 nm; em: 578 nm The first fluorescent dye used for measuring membrane potential; phototoxic agent 5-Octadecanoylaminofluorescein abs: 497 nm; em: 519 nm Cell membrane stain; used for staining latex and liposome Oxonol V [Bis-(3-phenyl-5-oxoisoxazol-4-yl) pentamethine oxonol] abs: 610 nm; em: 639 nm Fluorescence decrease upon membrane hyperpolarization Oxonol VI [Bis-(3-propyl-5-oxoisoxazol-4-yl) pentamethine oxonol] abs: 599 nm; em: 634 nm Prodan [6-Propionyl-2-dimethylaminonaphthalene] abs: 363 nm; Em (max): 497 nm Environment-sensitive dye for studying membranes and structures of proteins 1,3,6,8-Pyrenetetrasulfonic acid, tetrasodium salt abs: 374 nm; em: 403 nm Hydrophilic cell tracer Rhodamine 123 *UltraPure Grade* abs: 507 nm; em: 529 nm Widely used for measuring membrane potential of mitochondria; Fluorescence is less dependent on dye location Rhodamine B, hexyl ester, perchlorate abs: 556 nm; em: 578 nm Mitochondrial stain SITS [4-Acetamido-4'isothiocyanatostilbene- 2,2'- disulfonic acid, disodium salt] abs: 336 nm; em: 436 nm Fluorescent probe for studying ion transporter SP-DiIC18(3) [1,1'-Dioctadecyl-6,6'di(4-sulfophenyl) -3,3,3',3' tetramethylindocarbocyanine] abs: 556 nm; em: 573 nm Lipophilic neuronal tracer Qty 25 mg 84732 5 mg Cat. No. 84917 Product
FLUORESCENCE
Qty 5 mg
SP-DiOC18(3) [3,3'-Dioctadecyl-5,5'di(4-sulfophenyl) oxacarbocyanine, sodium salt] abs: 497 nm; em: 513 nm Lipophilic neuronal tracer SPEEDY DiA [4-(4-(Dilinoleylamino)styryl)-Nmethylpyridinium iodide] *Oil* abs: 492 nm; em: 612 nm Fluorescent lipophilic tracer TMRE [Tetramethylrhodamine, ethyl ester, perchlorate] abs: 549 nm; em: 574 nm Used for measuring membrane potential of mitochondria; Fluorescence is less dependent on dye location. TMRM [Tetramethylrhodamine, methyl ester, perchlorate)] abs: 549 nm;em: 573 nm Used for measuring membrane potential of mitochondria; Fluorescence is less dependent on dye location.
5 mg
88060
88061
25 mg
88201
25 mg
88065 25 mg
25 mg
84720
88210
100 mg
84703
100 mg
Cell-Impermeant Nucleic Acid Stains Used for Cell Viability Assays

Cat. No. 83208 Product EthD-1 [Ethidium homodimer-1] abs: 528 nm; em: 617 nm Higher affinity to nucleic acids than EtBr EthD-2 [Ethidium homodimer-2], 1 mM solution in DMSO abs: 535 nm; em: 624 nm Higher affinity to nucleic acids than EtBr Ethidium Bromide, 5 mM aqueous solution abs: 518 nm; em: 605 nm Ethidium Bromide *UltraPure Grade* properties same as above Ethidium monoazide bromide abs: 462 nm; em: 625 nm A fluorescent photoaffinity label that couples covatently to nucleic acids upon light irradiation Propidium iodide abs: 535 nm; em: 617 nm Propidium iodide, 1.0 mg/mL solution in water properties same as above Stains-All abs: 573 nm; em: 609 nm Differential staining of nucleic acids and proteins; RNA (bluish purple), DNA (blue) and proteins (red) Qty 1 mg
84704
100 mg 83209
200 ml
88212
25 mg 83222 83221 100 mg 83214 25 mg 83212 83215 10 mg 83013 100 mg
10 x 1 mL 1g 5 mg
88310
88054
25 mg 10 ml 100 mg
88058
88316
84918
5 mg
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LDH based Assay Kits for Analyzing Cell Viability & Proliferation
DHL Cell Viability and Proliferation Assay Kit *Fluorimetric*
Lactate dehydrogenase (LDH) generally exists in the cytoplasm of living mammalian cells. The measurement of cytoplasmic LDH activity is a wellaccepted assay to quantify living cell numbers and indicate cell viability. The kit provides researchers a one-step assay to count living cells in a culture and continuously monitor cell proliferation over time by measuring cytoplasmic LDH activity. The kit uses resazurin as a sensitive fluorogenic indicator, which is converted to resozufin (excitation/ emission=560nm /590 nm) by cytoplasmic LDH3-5. It can detect as little as 48 living cells with the linear range up to 5X104 cells (r2=0.99). It is suitable for high throughput screening of cell proliferation or cytotoxicity effect of a variety of compounds. Cat. No. 71300 Product DHL Cell Viability and Proliferation Assay Kit *Fluorimetric* DHL Cell Viability and Proliferation Assay Kit *Fluorimetric* Qty 2000 assays 10,000 assays
FLUORESCENCE
Assay kits for Cell Viability/Cytotoxicity
71301
DHL Express Cell Counting Kit *Fluorimetric*

DHL Express Cell Counting Kit provides a convenient 15-minute assay to quantify the total cell number (including live cells and dead cells) by measuring total LDH activity in cytoplasm and culture medium. Upon cell death, LDH is released into surrounding medium. The kit uses resazurin, a sensitive indicator for cell viability. Resazurin is converted to resozufin (excitation/emission=560 nm/590 nm) by LDH with a coupled enzymatic reaction. The kit can detect as little as 97 cells with the linear range up to 2.5X104 cells (r2=0.95). It is suitable for high throughput screening of cell proliferation or cytotoxicity effect of a variety of compounds. The kit contains: Assay mixture, Assay buffer, Lysis solution, Stop solution, A detailed protocol Cat. No. 71405 Product DHL Express Cell Counting Kit *Fluorimetric* DHL Express Cell Counting Kit *Fluorimetric* Qty 500 assays 5000 assays
71406
DHL Cell Cytotoxicity Assay Kit *Fluorimetric*

The damage of cell membrane leads to the release of cytoplasmic enzymes. The measurement of released cytoplasmic lactate dehydrogenase (LDH) is a well-accepted assay to estimate cell membrane integrity and quantify cytotoxicity. The EnzoLyte Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon converted) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but it only measures the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit contains: Assay mixture Assay buffer Lysis solution Stop solution A detailed protocol
Cat. No. 71302
Product DHL Cell Cytotoxicity Assay Kit *Fluorimetric* DHL Cell Cytotoxicity Assay Kit *Fluorimetric*
Qty 500 assays 5000 assays
71303
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FISH Paints
STAR*FISH Chromosome Painting
The Cambio STAR HFISH Chromosome Painting System has been developed in collaboration with a leading molecular cytogenetics laboratory in the University of Cambridge, U.K. The products come with Cambios guarantee of quality, and are backed with the full technical support of the Cambridge University molecular cytogenetics laboratory. Cambios Chromosome Paints are uniquely prepared from PCR* amplified DNA obtained from FACS-sorted chromosomes. F. Hoffmann-La Roche Inc. has granted Cambio a PCR based DNA manufacturing license to make the STAR HFISH range of Chromosome Paints. The STARHFISH range includes: Mouse Whole Chromosome-specific Probes, Human and Mouse Centromeric Probes, Detection Kits, Labeled and Secondary Antibodies and ancillary products. We are also pleased to announce that this range is now complemented by AL Technologies products, which include Human Chromosome-specific p and q arm probes.
CYTOGENETICS
StarFish chromosomal Paints
STAR*FISH Human Band Probes
Chromosome-Specific
These specific band and oncogene probes are constructed from microdissected normal human metaphase chromosome DNA that has been directly amplified by PCR using a degenerate primer. The probes are available labelled with either biotin or digoxigenin (DIG).
STAR*FISH Human Chromosome-Y-Specific Probes

Chromosome Y Euchromatic Probe Biotin Labelled This probe is an unpublished cosmid clone (LOR2.6, 9, Taylor & Wolfe), which can be used to detect the euchromatic portion of the Y chromosome but not the pseudoautosomal region at the distal tip of Yp (and Xp) and not the distal Yq heterochromatin. It gives no signal elsewhere on the genome. Use detection kit numbers 1066-K, 1082-KT, 1089-KB, 1090-KD, 1043-KB or 1596-KC with this product. Chromosome Y Heterochromatic Region Probe To supplement our Chromosome Y Euchromatic probe and the whole chromosome Y paints, which do not paint heterochromatin, we also provides a Yqh-specific probe. Available with biotin, FITC or Cy3 labelling, it can be used in conjunction with other STARHFISH paints and probes, and other STAR*FISH detection kits and reagents.
STAR*FISH Human Whole ChromosomeSpecific Probes

Cambios STAR*FISH system provides an excellent method for the identification of single human chromosomes, and allows the detection of translocations and insertions on metaphase chromosomes. Marker chromosomes can also be identified and complex karyotypes analysed. Probes are available for all chromosomes. To allow users to undertake more ambitious protocols, paints are now supplied in concentrated form with separate hybridisation buffer. The individual paints come in packs of 10 and 20 tests. All Cambio paint systems for whole human chromosomes are now Cot-1 free. Ground breaking work by our collaborators in the University of Cambridge has resulted in competitor DNA-free painting. AL Technologies whole chromosome probes labelled with digoxigenin (DIG) are now available from Cambio.
STAR*FISH Human Chromosome-Specific Centromeric Probes

A comprehensive range of biotin, Cy3 & FITC labelled human chromosome-specific centromeric probes is now available from Cambio.
STAR*FISH Human Paint Boxes

The Cambio Paint Box contains 3 tests for each chromosome presented in one convenient palette, which is particularly useful for researchers needing to test for each chromosome. STAR*FISH Paint Boxes are supplied in a ready-to-use format (45l per vial).
STAR*FISH Human Chromosome Pan Centromeric Probes

These DNA probes identify the centromeric region of each chromosome. They are ideal for the detection of aneuploidy and polyploidy, dicentrics, tricentrics and other complex aberrations, as well as for general numerical chromosome analysis. All STAR*FISH Chromosome Paint systems are carefully tested for their cytogenetic performance as in situ hybridisation probes The paints are available in both concentrated and ready-to-use format. Storage at -20C is recommended.
Multicolour FISH (M-FISH)

Multicolour FISH (M-FISH) allows the simultaneous visualisation of all human chromosomes in a different colour and consequently allows the examination of the human genome in a single hybridisation. The technology is especially useful when specimen material is limited and numerous complex chromosomal rearrangements are involved. SKY and M-FISH are the two systems used to analyse multicoloured chromosomes and the systems differ only in the way they capture and analyse the images. The multicolour probe set consists of a mixture of combinatorially labelled chromosomespecific paints of all human chromosomes. These paints are derived from DOP-PCR-amplified DNA from FACS-sorted chromosomes.
STAR*FISH Human Chromosome Pan-Telomeric Probes

We offer a probe specific to the telomeric region of all human chromosomes. Implicated in the processes of cell division, telomeres are believed to play a role in senescence and cancer. STAR*FISH telomeric probes are an invaluable tool to any researcher studying these areas. Available labelled with biotin, FITC or Cy3 the probes are tested for specificity and signal intensity, and are provided with full experimental protocols.
Human Rainbow*FISH Probes

The human Rainbow*FISH probes visualise 6 chromosomes per set (1-6, 712, 13-18 and 16-Y). Covering the entire genome in just four experiments, the Rainbow*FISH probes are quick and easy to use. The probes utilise biotin, FITC and Cy3, so there is no need to buy new filters or software. The probes come ready to use with hybridisation buffer.
STAR*FISH Human Chromosome-Specific Arm Probes

AL Technologies probes for the p or q arm of each chromosome are labelled with either biotin or digoxigenin (DIG). Please note that due to cross-hybridisation only the q arm is available for chromosomes 13, 14, 15, 21 and 22.
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FISH Paints
Harlequin*FISH Paints
Harlequin*FISH paints are derived from the application of chromosome painting to comparisons of the genetics of a wide variety of species. This cross-species colour banding was developed to be applied to the analysis of the human genome. Created from two closely related gibbon species, the probes hybridise to different loci in the human genome. Benefits: Whole genome analysis in a single multicolour FISH assay Combinatorial labelling generates 7 colours Unique bar code for each chromosome Characterise gross and cryptic genomic rearrangements Detect inter-chromosomal translocations Interpret marker chromosomes and samples with poor morphology Provide rapid and precise characterisations of leukaemias Detect and classify cancers Monitor therapeutic response Accessible, robust FISH tool
CYTOGENETICS
StarFish chromosomal Paints
Mouse Rainbow*FISH Probes

The mouse Rainbow*FISH probes visualise 7 chromosomes per set (1-7, 814 and 15-Y). Covering the entire genome in just three experiments, the RainbowHFISH probes are quick and easy to use. The probes utilise biotin, FITC and Cy3, so there is no need to buy new filters or software.
X-Y FISH Bovine Sex Test Kit

X and Y chromosome paints were developed from sorted yak chromosomes for sexing cattle spermatozoa. The procedure was evaluated using the Beltsville sperm sexing technology, which separates spermatozoa by flow-cytometry into X- and Y-bearing fractions. Using FISH, the method is a simple, reliable and robust procedure for assessing the effectiveness of any separation technique of bovine X and Y spermatozoa. Paints can also be used for interphase-FISH.
Bovine Translocation (1;29) FISH Kit

The 1;29 balanced chromosome translocation is recognised to be the most common chromosome aberration in cattle causing reduced fertility. The t(1;29) abnormality can be identified or excluded by conventional chromosome analysis, which is used widely as a screening test. FISH analysis offers the advantage of identifying the translocation in interphase nuclei and suboptimal chromosome preparations. It provides a rapid and unequivocal diagnosis.
STAR*FISH Primed In Situ Synthesis

Telomeric PRINS Kit STAR*FISH PRINS (PRimed IN situ Synthesis) is a rapid and sensitive method of detecting sequences of DNA without requiring lengthy in situ hybridisation protocols. The technique is basically a single-cycle PCR reaction, requiring a single specific primer and labelled dUTP/dNTP solution. The key advantages of this method of in situ detection are the intensity and clarity of the signal and the speed of the protocol. The signal produced is intense and localised due to the fact that individual unincorporated nucleotides do not contribute significantly to background, and carefully chosen primers eliminate false signal emerging from other loci. With its speedy protocol, results can be obtained in under 1 hour (a lot faster than conventional FISH). Also, PCR requires few, if any, toxic reagents, unlike the need for formamide in FISH.
STAR*FISH Rat 12/y Paints STAR*FISH Pig x/y Paints

These highly-specific paints are suitable for a wide range of applications. As with all our paints, they come with the Cambio guarantee of quality, and the full technical support of the University of Cambridge Molecular Cytogenetics Laboratory.
STAR*FISH Detection Kits

These Detection Kits have been specially designed to optimise the visualisation of the Chromosome Paints. Due to popular demand, they are provided separately from the Paints.
STAR*FISH Mouse Whole Chromosome-Specific Probes

STAR*FISH Mouse Whole Chromosome-Specific paint systems are prepared from PCR-amplified DNA obtained from FACS-sorted chromosomes. Mouse paints are now being used to develop model systems for studies in genetics, mutagenesis, developmental cancer biology, and as human surrogates in studying the effects of genotoxic agents. Available in this range are whole chromosome paints for use in the detection of translocation and trisomy in mice. Paints are available for chromosomes 119, X and Y. Mouse probes are available labelled with biotin, FITC or Cyanine 3, and are supplied with hybridisation buffer in packs of 10 and 20 tests. Unless otherwise requested, mouse probes are supplied in the ready-to-use format.
Sonicated Human Genomic DNA

This product has been purified from human placental by phenol extraction. Quality control (by electrophoresis) indicated the absence of protein and RNA. It is suitable for eliminating background when performing Southern and other types of hybridisation, genomic analysis, and library construction. Size range shows a medium 300500bps. Supplied at 10mg/ml.
Proteinase K & Ribonuclease A (RNase A)

Please see page # 3 and page # 39
STAR*FISH Mouse Paint Boxes

In addition to our popular human paint boxes, we also offers 3 tests of each mouse whole chromosome paint in one convenient palette. This product was specifically designed for researchers needing to test for each chromosome, and is also a useful resource when initialising a new painting assay or service
STAR*FISH Mouse Pan-Centromeric Probes

To complement our human pan-centromerics, ourrange includes mouse pan centromeric probes. Manufactured and quality controlled to the same high standards as our human probes, the mouse pan-centromerics are ideal for the detection of chromosome number aberrations in this experimental species.
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Genome Walking- APATechnology

Asymmetrical PCR Amplification (APA) technology
NEW TECHNOLOGIES
APA Gene Technology
Asymmetrical PCR amplification (APA) represents a new generation of PCR technology developed by Bio S&T (U.S. patent pending). PCR technology is widely used in molecular biology for DNA cloning, genomic typing and sequencing. It has also been applied in medical and pharmaceutical research, clinical diagnosis and human DNA fingerprinting. A variety of PCR permutations have been developed since its conception. However, a common limitation among these variations is the need to know the DNA sequences flanking the region of interest. Thus, DNA amplification has been limited to templates with known sequences. APA is designed specifically to overcome this limitation. APA technology is capable of selectively or randomly amplifying any unknown DNA sequences. The APA procedure includes two steps (see figure 1):
In step 1, particular primer sets named degenerated random tagging (DRT) primers are involved in the reaction. Each DRT primer consists of three components respectively, each containing a 4-6 nucleotide arbitrary sequence at the 3 end, a bi-nucleotide degenerated sequence and a tagging primer binding sequence. The functions of the DRT primers are to randomly bind denatured DNA templates, lead single strand DNA amplification, and provide a tagging primer binding position in the 5 region. The annealing temperature during this step ranges from 35-42 oC with an optimum of 37 oC for many species. In step 2 of the APA procedure, a specific primer and tagging primer are used in a reaction performed at a higher annealing temperature (higher than 57oC). One of the most effective uses of APA Technology is gap filling in genome integration. Conventional gap filling is often laborious, time consuming and ineffective given that too much DNA template is utilized in each reaction. Through APA Technology the sequencing process to walk the BAC clones is extremely simple and reliable with the additional advantage that only a minute amount of DNA is needed for the reaction. Usually, BAC End sequencing is usually limited by two factors: 1) a large amount of DNA, usually more than 5 ug is needed for each reaction 2) the BAC vector is typically a single copy vector, making it hard to extract large amounts of DNA from a BAC clone. Our revolutionary APA Technology has overcome these limitations. With this absolutely fresh approach, only 2-5 ng of template DNA are needed for each reaction, almost 1000 times less than required by the conventional procedure. Figure 1: Schematic Representation of the APA Technology
APAgene Genome Walking Kit

Can be used for: gap filling, localized cloning of genomic DNA, isolating promoter and regulatory sequences corresponding to cloned cDNAs, 5 and 3 RACE of first-strand cDNAs, and identifying intron/exon junctions.
APAgene Locator Kit

Can be used for: identifying transgene/genomic DNA junctions, bi-directional sequence extension of sequence-tagged sites (STSs) and expressed sequence tags (ESTs), identifying gene traps and transposon-insertion locations.
APAgene BAC End Amplification Kit

Can be used for: insert-end amplification of large clones such as P1, YAC and BAC as well as localized sub-cloning of large clones such as P1, YAC and BAC. Cat. No. BT600 Product APAgene Genome Walking Kit APAgene Genome Walking Kit APAgene Genome Walking Kit Qty 6 walks Cat. No. BT700 Product APAgene Genome Locator Kit APAgene Genome Locator Kit APAgene Genome Locator Kit Qty 6 walks Cat. No. BT500 Product APAgene BAC End Amplification Kit APAgene BAC End Amplification Kit APAgene BAC End Amplification Kit Qty 6 walks
BT601
20 walks
BT701
20 walks
BT501
20 walks
BT602
40 walks
BT702
40 walks
BT502
40 walks
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A Novel Cloning Vector - pLivSelectCloning Technology

pLivSelectCloning Technology
pLivSelect technology (patent pending No. 2,335,412) is the latest technology in DNA cloning. Compared with conventional DNA cloning technologies, which depend on blue/white selection for recombinants, pLivSelect is a revolution in DNA cloning. In conventional cloning technologies, the lac operon contains a partial lacZ sequence coding for the N-terminal portion of the b-galactosidase gene. The C-terminal portion is contributed by the host via a-complementation. If a DNA insert is not introduced into the lacZ gene, an enzymatically active b-galactosidase will be generated via the a-complementation of the N-terminal portion with the C-terminal portion contributed by the host strain. The functional b-galactosidase will then cleave X-gal resulting in an insoluble blue substance forming blue colonies. If a DNA insert is introduced into the lacZ gene, transcription of the N-terminal portion of the b-galactosidase will be interrupted, preventing a-complementation and consequently cleavage of X-gal. The resulting colonies are colorless. Our revolutionary pLivSelect cloning vector provides an innovative direct selection system for recombinant identification. The key part of this innovative cloning system comprises:
NEW TECHNOLOGIES
Cloning Vector
pLivSelect cloning vector

The pLivSelect -PCR vector is derived from the pLivSelect -I vector (Cat#BC101). The XbaI site in the MCS region of the original vector has been replaced by a Pml I site. Therefore, the pLivSelect-PCR vector contains two Pml I sites flanking the PCR insertion site ( Hpa I) so that the PCR insert can easily be excised from the vector through a single Pml I digestion. The pLivSelect -PCR vector provided in the pLivSelect -PCR Cloning Kit has been linearized using Hpa and processed through a unique procedure so that the DNA vector can be used to clone all types of PCR products. Therefore, pLivSelect -PCR Cloning Kits can be applied for cloning PCR products amplified using either Taq or Pfu DNA polymerases (those which generate either sticky or blunt ends). Choose between pLivSelect -II Cloning Kit A, B or C depending upon the restriction sites into which you would like to clone your gene. The following are the contents of pLivSelect -II Cloning Kit A, B and C: Linearized and dephosphorylated pLivSelect-II vector DNA, 100 ng/l T4 DNA ligase, 5 U/l 10X T4 ligase buffer E. coli DH10B glycerol stock
A lacI repressor sequence that has been engineered to contain multiple cloning sites (MCS); A lac-ABR operon where the antibiotic resistant gene is placed under the regulation of the lac operon.
pLivSelect-II Cloning Kit R-A, R-B and R-C

Contains 40 Ready-to-go cloning reaction mixtures (for a 20 l ligation reaction). Each lyophilized reaction mix contains 100 ng of pLivSelect.-II vector DNA T4 ligase buffer components T4 DNA ligase E. coli DH10B agar stock
If there is no DNA insertion in the MCS area of the lacI sequence, lacI has normal repressor function that will inhibit lac promoter activity for blactamase synthesis. Therefore, cells that harbor the non-recombinant vector cannot survive on agar plates containing chloramphenicol. In contrast, if a DNA insert has been introduced into the MCS region of the lacI sequence, the chlorampehnicol resistance gene will be normally expressed owing to the absence of lacI repressor activity. Cells that contain a vector carrying an exogenous DNA fragment will survive on agar plates containing the antibiotic. pLivSelect is thus a direct antibiotic-based selection system for recombinant identification. pLivSelect-PCR Cloning Kits can be applied for cloning PCR products amplified using either Taq or Pfu DNA polymerases (those which generate either sticky or blunt ends).
pLivSelect-II-PCR Cloning Kit L

(With and Without competent cells) Optimized to enhance blunt-end ligation (all types of PCR products with blunt ends or 3-A overhang sticky ends can be cloned with high efficiency).
pLivSelect-II-PCR Cloning Kit

Optimized to enhance blunt-end ligation (all types of PCR products with blunt ends or 3-A overhang sticky ends can be cloned with high efficiency). Transformation q All E. coli strains that do not express the lac repressor ( lacI or lacI ) may be used for transformation. If you use a strain that expresses the lac repressor, your transformants WILL NOT grow on agar plates containing chloramphenicol.
Figure 1: PLivSelect Vector Map
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A Novel Eukaryotic Expression System-LEXSY

Leishmania Expression System
NEW TECHNOLOGIES
Eucaryotic Expression System
The Leishmania Expression System represents the combination of easy handling known from bacterial expression systems with the potential of an eukaryotic protein expression/folding/ modification system. The protozoan host Leishmania tarentolae was isolated from lizard and is non-human pathogenic (Biosafety level 1). For high level expression target genes are supplied with specific splicing signals and integrated into a strongly transcribed chromosomal site or maintained episomally. The expression constructs are made in E. coli and introduced into L. tarentolae by electroporation. Up to four selection markers can be used simultaneously for coexpression of multisubunit proteins in one strain or expression enhancement. General and secretory expression vectors are available. The selected recombinant strains can be cultivated as static or agitated cultures and grow to high cell densities in standard bacteriological media without sera. The cells can be easily lysed by sonication or detergents. One main advantage of the Leishmania Expression System is the potential for mammalian-type posttranslational modification of heterologous target proteins, such as glycosylation, phosphorylation, prenylation etc. Amplification of target gene from cDNA as Bgl II / Nco I / Xho I x Xho I / Pac I / Not I compatible cassette Insertion of target gene into Leishmania expression vector Transfection of Leishmania tarentolae host strain by electroporation Selection of recombinant strains with Nourseothricin/Hygromycin/Bleomycin/Neomycin Confirmation of genetic structure of recombinant strain by diagnostic PCR Cultivation of expression strain and evaluation of expression level of target protein Purification and characterization of target protein
Reference: Breitling et al. (2002) Non-pathogenic trypanosomatid protozoa as a platform for protein research and production. Prot. Expr. Purific. 25 :209.
Fig. 1 : The pF4X1.4 general expression plasmid family is available with sat, hyg, ble or neo marker genes for selection with Nourseothricin, Hygromycin B, Bleomycin or Neomycin. Instead of a poly-linker, the target gene replaces a stuffer fragment, allowing control for proper restriction. The Nco I site overlaps the ATG of the target gene. 5 int and 3 int are regions for homologous recombination into the host chromosome following linearization of the expression plasmid with Swa I; utr1, utr2 and utr3 are optimized gene flanking non-translated regions providing the splicing signals for posttranscriptional mRNA processing for expression of target and marker genes in L. tarentolae.
Figure 2: The pSP1.4sat secretory expression plasmid family allows the use of a signal peptide which has proven to function for extracellular secretion and correct processing in L. tarentolae . Signal peptide coding regions from human epo gene or from L. tarentolae gp63 gene are available. Instead of a polylinker, the mature part of the target gene replaces a stuffer fragment, allowing control for proper restriction. The Kas I site overlaps the signal peptide cleavage site. 5' int and 3' int are regions for homologous recombination into the host chromosome following linearization of the expression plasmid with Swa I. For secretory expression with the homologous signal peptide of the target protein the pF4X1.4 general expression plasmid family (Fig. 1) can be used.
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Protein Expression in a new system-LEXSY

Proteins expressed in L. tarentolae at Jena Bioscience:
Cytosolic proteins: human transcription factors of the proto-oncogene family: Miz-1, c-Myc, Max, Mad1, human Cu/Zn superoxide dismutase, -galactosidase, T7 RNA polymerase, green fl uorescent protein, glutathione S-transferase. Membrane proteins: human bradykinin receptor, Rab7-GTPase, Type I MBP. Secreted proteins: human erythropoietin, single chain antibodies, human interferon
NEW TECHNOLOGIES
Eucaryotic Expression System
High level Inducible expression of target proteins in LEXSY

Use of heterologous T7 polymerase-TET repressor system for high level protein synthesis: Cat. No. EGE-120 Product Inducible LEXSY Starter Kit bleomycin selection (contains vector pTUBAPX1.4ble) Inducible LEXSY Starter Kit neomycin selection (contains vector pTUBAPX1.4neo) Qty
1 Kit
EGE-121
1 Kit
Gene Expression Starter Kit

If you prefer to perform the complete transfection and expression procedure yourself, Jena Bioscience offers a Starter Kit, containing all essential components to engineer a Leishmania expression strain. The following steps are described in an easy to follow manual: 1. Amplifi cation of target gene as Bgl II / Nco I / Xho I x Xho I /Pac I / Not I compatible cassette 2. Insertion of target gene into Leishmania expression vector by standard methods in E. coli 3. Transfection of Leishmania tarentolae host strain by electroporation 4. Selection of recombinant strains with Nourseothricin / Hygromycin / Bleomycin / Neomycin 5. Confirmation of genetic structure of recombinant strain by diagnostic PCR 6. Cultivation of expression strain and evaluation of expression level of target protein Cat. No. EGE-101 Product Constitutive LEXSY Starter Kit bleomycin selection (contains vector pF4X1.4ble) Constitutive LEXSY Starter Kit hygromycin selection (contains vector pF4X1.4hyg) Constitutive LEXSY Starter Kit neomycin selection (contains vector pF4X1.4neo) Constitutive LEXSY Starter Kit nourseothricin selection (contains vector pF4X1.4sat) Qty
Expression vectors also available separately
Secretory expression of target proteins in LEXSY

Efficient protein secretion with function proved signal peptides for protein targeting: Cat. No. EGE-110 Product Secretory LEXSY Starter Kit EPO signal peptide nourseothricin selection (contains vector pF4SPepoX1.4sat) Secretory LEXSY Starter Kit LMSAP signal peptide nourseothricin selection (contains vector pF4SPlmsapX1.4sat) Secretory LEXSY Starter Kit LMSAP signal peptide hygromycin selection (contains vector pF4SPlmsapX1.4hyg) Secretory LEXSY Starter Kit LMSAP signal peptide neomycin selection (contains vector pF4SPlmsapX1.4neo) Qty 1 Kit
EGE-111
1 Kit
EGE-113
1 Kit
EGE-114 1 Kit
1 Kit
EGE-102
1 Kit
Expression vectors also available separately
EGE-103
Leishmania tarentolae Cultivation Starter Kits

1 Kit The Leishmania tarentolae Cultivation Starter Kit is the easiest way to establish a Leishmania tarentolae cell culture in your own laboratory. The kit contains everything to start your work with Leishmania tarentolae (strain as live suspension culture, media, cell culture flasks and cryo vials with glycerol). Cat. No. LT-102 Product LEXSY Cultivation Starter Kit P10 (contains laboratory strain P10; use for constitutive and secretory expression vectors) LEXSY Cultivation Starter Kit T7-TR (contains T7-TR strain expressing bacteriophage T7 RNA polymerase and TET repressor; use for inducible expression vectors) Qty 1 Kit
EGE-104
1 Kit
Kit
Contents Leishmania tarentolae recipient strain as live submerse culture 50 ml completed medium for initial inoculations Components for preparation of 1-liter growth medium General Expression vector with sat marker (Nourseothricin selection) 50 ml electroporation buffer Primer sets for insert sequencing and diagnostic PCR Manual (the manual is also available for download in PDF format from our web site at www.jenabioscience.com ) Cat. No. EGE-201 EGE-202 EGE-203 EGE-204 Product Qty
LT-111
1 Kit
pF4X1.4ble Constitutive expression vector 5 g (50 l) pF4X1.4hyg Constitutive expression vector 5 g (50 l) pF4X1.4neo Constitutive expression vector 5 g (50 l) pF4X1.4sat Constitutive expression vector 5 g (50 l)
Host strains, Growth media, antibiotics, additives and primers available. Please inquire for pricing of custom transfection services Important Licensing Information: This service is licensed for non-commercial research only. Commercial use of this expression system requires separate licensing. Cloning of customer genes in Leishmania expression vectors, development of protein purification protocols, pilot expressions and protein purification can be performed with a separate contract.
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INDEX
1 kb Ladder ................................................................ 11 100 bp + 1.5 kb DNA Ladder ............................................ 11 100 bp Ladder ............................................................. 11 2-Deoxyribonucleoside-5-Triphosphate Solutions ................ 11 200 bp Ladder ............................................................. 12 293 cell nuclear extract ................................................ 123 2'-dATP ...................................................................... 11 2'-dCTP ...................................................................... 11 2'-dGTP ...................................................................... 11 2'-dNTP (mix of all 4) .................................................... 11 2'-dTTP ...................................................................... 11 2'-dUTP ...................................................................... 11 2'-Fluoro-dCTP Solution .................................................. 32 2'-Fluoro-dUTP Solution .................................................. 32 2-Way Cap, m7G[5]pppp[5]m7G .................................. 31 2X Tissue & Cell Lysis Buffer ............................................. 3 3'-RACE ............................................................... 132,151 50 bp Ladder ............................................................... 12 500 bp Ladder ............................................................. 12 5'-RACE ............................................................... 132,151 8-Oxoguanine-DNA Excision Mix ........................................ 41 Abl SH3 domain .......................................................... 106 ABTS ......................................................................... 91 acridine orange .......................................................... 137 actinomycin D ............................................................ 137 Additive screen ............................................................ 79 Additives .................................................................... 79 ADHP ......................................................................... 91 adrenergic receptors .................................................. 101 Adrenocorticotrophin peptides ...................................... 130 AEC fizzing tablets ...................................................... 113 AF6-RBD ................................................................... 100 Affinity chromatography resins, columns ............................ 58 Agarase ...................................................................... 17 Agarose Gel-Digesting Preparation, GELase ....................... 17 Agouti related peptides ............................................... 130 Akt1/PKBa ........................................................... 106-110 Akt1/PKBb ........................................................... 106-110 Alcian Blue ................................................................ 141 Alkaline Phosphatase (CIP) .............................................. 42 Alkaline Phosphatase assay kits ......................................... 89 Alkaline Phosphatase, APex Heat-Labile ............................ 42 Alkaline Phosphatase ...................................................... 42 Amino acids ............................................................... 127 Amino acids-Boc labelled .............................................. 127 Amino acids-Fmoc labelled ............................................ 127 Amino hexyl CTP Sepharose ............................................ 58 Amino hexyl dCTP Sepharose .......................................... 58 Amino hexyl dGTP Sepharose .......................................... 58 Amino hexyl dITP Sepharose ........................................... 58 Amino hexyl dUTP Sepharose .......................................... 58 Amino hexyl dXTP Sepharose .......................................... 58 Amino hexyl GTP Sepharose ............................................ 58 Amino hexyl ITP Sepharose ............................................. 58 Amino hexyl UTP Sepharose ............................................ 58 Amino hexyl XTP Sepharose ............................................ 58 Amino hexyl ATP Sepharose .............................................. 58 Amino hexyl dATP Sepharose ........................................... 58 Amino octyl ATP Sepharose .............................................. 58 Amino octyl CTP Sepharose ............................................. 58 Amino octyl dATP Sepharose ............................................ 58 Amino octyl dCTP Sepharose ........................................... 58 Amino octyl dGTP Sepharose ........................................... 58 Amino octyl dITP Sepharose ............................................ 58 Amino octyl dUTP Sepharose ........................................... 58 Amino octyl dXTP Sepharose ........................................... 58 Amino octyl GTP Sepharose ............................................. 58 Amino octyl ITP Sepharose .............................................. 58 Amino octyl UTP Sepharose ............................................. 58 Amino octyl XTP Sepharose ............................................. 58 Amino phenyl ATP Sepharose ............................................ 58 Aminoallyl- UTP ............................................................ 34 Ammonium Acetate 5M Solution ........................................ 17 AmpliCap-MAX SP6 High Yield Message Maker RNA Kit .................................................. 31 AmpliCap-MAX T3 High Yield Message Maker RNA Kit ........... 31 AmpliCap-MAX T7 High Yield Message Maker RNA Kit ............................................................. 31 AmpliCap SP6 High Yield Message Maker RNA Kit ................. 31 AmpliCap T3 High Yield Message Maker RNA Kit .................. 31 AmpliCap T7 High Yield Message Maker RNA Kit .................. 31 Amplification, DNA see PCR Ampligase DNA ligase Kit ............................................... 43 Ampligase Thermostable DNA Ligase ................................. 43 AmpliScribe SP6 High Yield Transcription Kit ..................... 30 AmpliScribe T3 High Yield Transcription Kit ...................... 30 AmpliScribe T3-Flash Transcription Kit ........................... 30 AmpliScribe T7 High Yield Transcription Kit ...................... 30 AmpliScribe T7-Flash Transcription Kit ........................... 30 AmpliTherm DNA Polymerase, MasterAmp ........................ 8 Amyloid - vital stains ..................................................... 93 Amyloid peptides ........................................................ 130 Analytical services ...................................................... 133 AnaPrep AEC substrate system ....................................... 115 AnaPrep IHC Histain kit ................................................ 115 ANS ......................................................................... 141 Antibiotic ................................................................. 130 Antibodies, custom synthesis ......................................... 133 antibodies, primary .................................................... 113 antibodies, secondary .................................................. 113 Antibody diluent ......................................................... 115 antioxidants .............................................................. 126 APA technology ........................................................... 151 APex Heat-Labile Alkaline Phosphatase .............................. 42 A-plus Poly (A) Polymerase Tailing kit ................................ 31 Apoptosis Blocking peptides .......................................... 130 Apoptosis ................................................................ 94-95 ARCA Cap Analog, 3-O-Methyl-m7G[5]ppp[5]G .................. 31 ARCA, 3-O-Methyl-m7G[5]ppp[5]G ................................ 19 aRNA Amplification .................................................... 33-35 ArrayPure Nano-scale RNA Purification kits ......................... 4 Arrest-in Transfection reagent ........................................ 36 artemin .................................................................... 125 ATP (Adenosine-5'-Triphosphate) ...................................... 32 ATP Solution ................................................................ 32 Autocamtide-2, substrate for CaMK ................................ 109 Autocamtide-3, substrate for CaMK ................................ 109 Automated Cycle Sequencing ........................................... 56 B stearothermophilus (rBst) DNA Polymerase ........................ 7 1-adrenergic receptor-G protein fusion ......................... 101 1-adrenergic receptors .............................................. 101 2-adrenergic receptors .............................................. 101 2-adrenergic receptors-G protein fusion ........................ 101 BAC Clones .................................................................. 28 BAC Cloning ............................................................. 19-21 BAC Genomic library ................................................... 131 BAC library construction .............................................. 131 BAC Vectors BACMAX DNA Purification kit ........................................ 4,20 Bacterial Protein Extraction Solution ................................. 57 Bacteriophage Lambda ............................................... 12,21 BAC-Tracker Supercoiled DNA Ladder .............................. 20 BAPTA ...................................................................... 144 BCIP/INT .................................................................. 113 Bcl-10 ...................................................................... 121 BDNF, brain derived neurotrophic factor ......................... 125 beadbeater ................................................................. 67 Benchtop freezer ......................................................... 66 Beta amyloid antibodies ................................................. 93 Beta amyloid fluorescence sampler kits .............................. 93 Beta amyloid peptides ................................................... 93 Beta amyloid tracers ..................................................... 93 Beta-secretase assay kit ................................................. 90 Beta-secretase assay-substrates ....................................... 90 bio pulverizer .............................................................. 68 Bioactive peptides ...................................................... 130 biocytin C2 maleimide .................................................. 114 Biocytin .................................................................... 114 Bioprocessing, Protein & Nucleic Acid ............................... 57 Biotin Quantitation kit, HABA ........................................ 115 Biotin, thiol reactive ................................................... 114 Biotin-fluorophore tagged ............................................. 115 Biotins, amino containing .............................................. 114 Biotins, amino reactive ................................................ 114 Biotin-X .................................................................... 114 Biotin-X-X-NHS ............................................................. 35 Biotinylation kit for proteins ......................................... 114 BioTrack Human Gene subsets .......................................... 25 BLA RP-1 Reverse Primer ............................................... 50 bLyS/BAFF, B-lymphocyte stimulator/B-cell activating factor 125 BMP-2, bone morphogenetic protein-2 ............................ 124 BMP-4, Bone Morphogenetic protein 4 ............................. 123 Bombesins ................................................................. 130 B O P ......................................................................... 128 BPDEtide, substrate for PKG ......................................... 109 Bradykinins ............................................................... 130
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INDEX
Brahma-related Gene 1 protein ...................................... 122 BRCA1 ...................................................................... 121 BrdU ....................................................................... 138 BrdUTP .................................................................... 138 Brucella ORF Collection ................................................... 26 BSA, maleimidyl .......................................................... 115 BuccalAmp DNA Extraction Kit ......................................... 1 Building Blocks ........................................................... 129 C jejuni ORF Collection ................................................... 26 C.elegans ORF Collection ................................................. 27 C.elegans Promoter Collection .......................................... 27 C.elegans RNAi library ................................................... 36 Calcein ..................................................................... 143 Calcitonin related peptides ........................................... 130 Calcium binding proteins .............................................. 126 Calcium indicators, non luminiscent ................................ 144 Calcium indicators, UV excitable .................................... 143 Calcium indicators, Visible light excitable ................... 143,144 Calcium/calmodulin-dependent kinases ............................. 107 Calmodulin ................................................................ 126 Calpain-substrate ......................................................... 87 Cancer research Blocking peptides ................................. 130 Candida albicans Genomic Library .................................... 27 Cap analogs ................................................................. 31 Capping Kits, RNA ......................................................... 31 Caspase 3 assay kit ....................................................... 94 Caspase 7 assay kit ....................................................... 94 Caspase antibodies ........................................................ 95 Caspase assay kits ......................................................... 94 Caspase assay substrates ................................................ 95 Caspase Profiling kit ...................................................... 94 Catch-All Sample Collection Swabs .................................... 1 Cathepsin- substrate ..................................................... 87 CCAAT-box-binding Transcription factor1 ......................... 121 CD40 ligand ............................................................... 125 cdc25A ..................................................................... 111 Cdc4 ........................................................................ 298 cDNA Clones ......................................................... 16,25-27 cDNA Cloning ............................................................ 16-17 cDNA Libraries ......................................................... 25-27 cDNA library construction ............................................. 131 cDNA Synthesis ......................................................... 14,15 Cell adhesion assays ..................................................... 133 Cell counting kit .......................................................... 148 Cell Cytotoxicity assay kit ............................................. 148 Cell permeable peptides ............................................... 130 Cell proliferation assays ............................................... 133 Cell viability and Proliferation assay kit ........................... 148 Cell viability assays, fluorogenic substrates .................. 145-148 Cell viability, monitoring cellular functions by fluorescent stains ............................................... 145-148 c-fos ........................................................................ 121 Chiller ........................................................................ 66 CHK2 (Checkpoint kinase 2) ........................................... 107 Chromosome paints ................................................ 149-150 CircLigase ssDNA Ligase .............................................. 122 c-jun ....................................................................... 121 CK 2 (Casein kinase 2) .................................................. 107 CK2a ................................................................... 107,108 CK2b .................................................................. 107,108 Cloning kit, PCR, pLivselect vector ................................. 152 Cloning vector, pLivselect ............................................. 152 c-myc ...................................................................... 121 c-myc-anti, antibody ................................................... 113 CNTF, Ciliary Nerotrophic factor .................................... 125 Coelentrazine ............................................................. 144 Colony Fast-Screen Kit (PCR Screen) ............................... 22 Colony Fast-Screen Kit (Restriction Screen) ..................... 22 Colony Fast-Screen Kit (Size Screen) ............................... 22 Competent Cells ........................................................ 23-24 Complement Component 5a receptors .............................. 103 Congo Red1 ................................................................. 41 CopyControl BAC Cloning Kit (BamH I) .............................. 19 CopyControl BAC Cloning Kit (EcoR I) ............................... 19 CopyControl BAC Cloning Kit (Hind III) ............................. 19 CopyControl cDNA, Gene & PCR Cloning Kit ....................... 16 CopyControl Fosmid Library Production Kit ...................... 18 CopyControl HTP Fosmid Library Production Kit ................ 18 CopyControl Induction Solution ............................... 16,18,19 CopyControl pCC1BAC BamH I Cloning Ready Vector ........ 19 CopyControl pCC1BAC EcoR I Cloning Ready Vector .......... 19 CopyControl pCC1BAC Hind III Cloning Ready Vector ..................................................... 19 CopyCutter EPI400 Cells ............................................. 23,24 Core Enzyme, E coli RNA Polymerase .................................. 29 Corticotrophin releasing factor ...................................... 130 Cosmid Cloning ............................................................. 21 Coupling reagents ....................................................... 128 c-Raf ................................................................... 99-100 CREBtide, substrate for PKA ......................................... 108 Crosstide, substrate for Akt/PKB ................................... 109 Cryo crystallization ....................................................... 82 Crystallization- Tape, Grease .......................................... 81 Crystallization-covers slides ............................................ 81 Crystallization-plates ..................................................... 81 CTP (Cytidine-5'-Triphosphate) ........................................ 32 CTP ........................................................................... 32 Custom gene synthesis .................................................. 131 Custom peptide synthesis ......................................... 130,132 Custom synthesis of Fluorescent probes ............................ 132 Cuvettes ................................................................. 73,75 Cycle Sequencing ........................................................ Kits Cyclin dependent kinases .............................................. 107 Cytochrome P450 assay substrates .................................... 92 Cytomegalovirus proteins .............................................. 117 Cytotoxicity assays ..................................................... 133 DAB ........................................................................... 91 Dansyl chloride ........................................................... 134 DAPI ........................................................................ 137 D-Biotin .................................................................... 114 DCC ......................................................................... 128 Detergents .................................................................. 79 DHFR-1 FP-1 Forward Sequencing Primer ..................... 52,55 DHFR-1 RP-1 Reverse Sequencing Primer ...................... 52,55 DIBOC ...................................................................... 128 DIC ......................................................................... 128 DIEA ........................................................................ 128 DMAP ....................................................................... 128 DNA & RNA Endonucleases ................................................ 40 DNA & RNA Exonucleases ................................................. 40 DNA Amplification, ................................................. see PCR DNA Binding Proteins ..................................................... 37 DNA Endonucleases ......................................................... 38 DNA Exonuclease I, E coli ................................................. 38 DNA Exonuclease III , E coli .............................................. 38 DNA Exonuclease VII, E coli .............................................. 38 DNA Exonucleases ...................................................... 38,39 DNA extraction ............................................................ 1-4 DNA Fragment 2X Precipitation Solution ............................. 17 DNA Glycosylases & Excision Mixes ..................................... 41 DNA Markers ............................................................ 11,12 DNA Polymerase I, E coli ................................................ 7-8 DNA Purifcation from Plant .............................................. 2 DNA Purification from Blood ............................................. 2 DNA Purification from Faeces ........................................... 2 DNA Purification from Leaf .............................................. 2 DNA Purification from Soil ............................................... 2 DNA Purification from Water ............................................ 2 DNA Purification .......................................................... 1-4 DNA Sequencing ........................................................ 55,56 DNase Af ..................................................................... 41 DNase, Plasmid-Safe ATP-Dependent .............................. 136 DNP ......................................................................... 139 dNTP ......................................................................... 11 Dopamine receptors .................................................... 102 Dr1, down regulator of transcription ............................. 1122 Drosophila Gene Collection .............................................. 26 Drosophila RNAi library .................................................. 36 DuraScribe SP6 Transcription Kit ................................... 32 DuraScribe T7 Transcription Kit ..................................... 32 Dynorphins ................................................................ 130 E coli RNA Polymerase Core Enzyme .................................. 29 E coli RNA Polymerase Holo Enzyme (sigma saturated) ........... 29 E coli DNA Endonuclease IV .............................................. 38 E2F-1 ....................................................................... 121 EasyLyseTM Bacterial Protein Extraction Solution ................. 57 EDANS ...................................................................... 134 EDC 128 EGF Receptor, substrate for MAPK ................................. 110 EGF, Epidermal Growth Factor ....................................... 125 EGTA ....................................................................... 144 EG-VEGF, Endocrine Gland-derived vascular endothelial growth factor .......................................... 125 Elastase-substrate ......................................................... 87
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INDEX
Electroblotter .......................................................... 62-63 Electrophoresis - horizontal, submarine ......................... 59-60 Electrophoresis- vertical ................................................ 61 Electrophoresis-power supplies ........................................ 61 Electrophoresis-Sequencing gels ....................................... 62 Electroporation Cuvettes ............................................ 22,73 ELISA substrates ......................................................... 113 Endexins ................................................................... 130 End-It DNA End-Repair Kit ............................................. 17 Endorphins ................................................................ 130 Endothelins ................................................................ 130 Enkephalins ................................................................ 130 Enzyme Storage Buffer ................................................... 12 Eosin ........................................................................ 141 EPAC-1 ....................................................................... 99 EpiFOS Fosmid Library Production Kit ............................. 19 Epi-Grids Colony Grid Templates ...................................... 22 EPO, erythropoetin ..................................................... 123 Epstein Barr Virus proteins .......................................... 117 ERK1 (extra cellular related kinase) ................................ 108 ERK2 (extracellular signal-regulated kinase) ...................... 108 Estrogen receptor ....................................................... 123 ESTs ........................................................................... 25 Ethidium bromide ....................................................... 137 Exo/S1 kit ................................................................... 41 ExtractMaster Fecal DNA Extraction kit ............................... 2 EZ::TN <blaM/R6K ori> Transposon ................................. 50 EZ::TN <DHFR-1> Insertion Kit ................................... 50,55 EZ::TN <DHFR-1> Tnp Transposome .............................. 52 EZ::TN <DHFR-1> Transposon ....................................... 52 EZ::TN <KAN-2> Insertion Kit ..................................... 50,55 EZ::TN <KAN-2> Tnp Transposome Kit ............................ 52 EZ::TN <KAN-2> Transposon .......................................... 52 EZ::TN <Not I/KAN-3> Transposon ................................... 54 EZ::TN <oriV/KAN-2> Insertion Kit .................................. 49 EZ::TN <oriV/KAN-2> Transposon .................................... 49 EZ::TN <R6K ori/KAN-2> Insertion Kit ............................. 50 EZ::TN <R6K ori/KAN-2> Transposon .............................. 50 EZ::TN <T7/KAN-2> Promoter Insertion Kit ....................... 49 EZ::TN <T7/KAN-2> Transposon ..................................... 49 EZ::TN <TET-1> Insertion Kit ......................................... 55 EZ::TN <TET-1> Transposon ........................................... 55 EZ::TN Beta-Lactamase Fusion Kit ................................... 50 EZ::TN In-Frame Linker Insertion Kit ............................... 54 EZ::TN Plasmid-Based Deletion Machine ............................ 54 EZ::TN pMOD-2<MCS> Transposon Construction Vector ................................................. 51 EZ::TN pMOD-3<R6K ori/MCS> Transposon Construction Vector .................................. 51 EZ::TN Transposase ................................................ 49-55 EZ::TN<R6K ori/KAN-2> Tnp Transposome ..................... 52 EZ::TNProtein Truncation Kit ........................................ 53 EZ-Tn5 <R6K ori/KAN-2> Insertion Kit ............................. 50 EZ-Tn5 <T7/KAN-2> Promoter Insertion Kit ....................... 49 EZ-Tn5 -Lactamase Fusion Kit ....................................... 50 FailSafe GREEN Real-Time PCR PreMix Selection Kit ........................................................... 10 FailSafe GREEN Real-Time PCR System .............................. 10 FailSafe PCR 2X PreMix A to Premix L ............................... 6 FailSafe PCR PreMix Selection Kit .................................... 6 FailSafe PCR System ..................................................... 6 FailSafe PROBES Real-Time PCR PreMix Selection Kit ................................................ 10 FailSafe PROBES Real-Time PCR System ........................... 10 FailSafe Real-Time GREEN Capillary PCR PreMix Selection Kit 10 FailSafe Real-Time GREEN PCR Capillary System ................. 10 FAM ......................................................................... 135 Farnesoid X-activated receptor ..................................... 123 Fast-Link DNA Ligation Kit ............................................. 43 FDG ........................................................................... 89 FDP, fluorogenic substrate for phosphatase ....................... 112 FDP ........................................................................... 89 FGF-11 ....................................................................... 21 FGF-21 ....................................................................... 21 FGF-91 ....................................................................... 25 Fibronectin fragments ................................................. 130 Finding Signal Peptides First strand synthesis .................................................... 14 FISH kit, bovine, (1,29) translocation ......................... 149-150 FISH probes, Harlequin ........................................... 149-150 FISH probes, Human chromosome Pan-telomeric ............................. 149-150 FISH probes, Human chromosome-arm specific .............................. 149-150 FISH probes, Human chromosome-specific band specific .................................................. 149-150 FISH probes, Human chromosome-specific centromeric .................................................... 149-150 FISH probes, Human chromosomeY-specific .................. 149-150 FISH probes, Human paint boxes ................................ 149-150 FISH probes, Human Rainbow .................................... 149-150 FISH probes, mouse paint boxes149-150 FISH probes, mouse, pan-centromeric probes .............. 149-150 FISH probes, Mouse, rainbow ................................... 149-150 FISH probes, mouse, whole chromosome-specific probes . 149-150 FISH probes, Multicolour FISH ................................... 149-150 FISH probes, pig, X/Y ............................................. 149-150 FISH probes, rat, 12/Y ............................................ 149-150 FISH probes, whole chromosome specific ..................... 149-150 FISH probes .......................................................... 149-150 FISH, detection kits ................................................ 149-150 FITC ........................................................................ 135 FLT3 ligand, Fms-like Tyrosine kinase 3 ligand .................... 123 Fluo-3 ...................................................................... 143 Fluo-5F ..................................................................... 143 Fluo-5N ..................................................................... 143 Fluorescamine ............................................................ 141 Fluorescent dyes .................................................... 134-141 Fmoc Biocytin ............................................................ 114 Fmoc-Osu .................................................................. 128 Formyl Peptide receptors ............................................. 102 Fosmid Cloning ......................................................... 18-19 Fosmid Library Production ......................................... 18,19 FosmidMAX DNA Purification kit ........................................ 4 Freezer storage boxes ................................................... 66 FRET probes, DABSYL based .......................................... 138 FRET probes, EDANS based ........................................... 139 Ftase ....................................................................... 100 Fugu IMAGE cDNA .......................................................... 26 Full Range DNA Ladder .................................................... 11 Fura-2 ...................................................................... 143 G ........................................................................... 99 GAL4-AH, GAL4 fused to alpha helix ................................ 121 GAL4-E1A .................................................................. 121 GAL4-Sp1Q ................................................................ 122 GAL4-VP16 ................................................................ 121 Galactosidase substrate ................................................. 89 Galanins .................................................................... 130 GAP (GTPase activation Protein) ...................................... 99 Gastrins ................................................................... 130 G-CSF ................................................................. 123,124 Gel Blot papers ............................................................ 63 Gel extraction .............................................................. 17 Gel scooper ................................................................. 76 GELase LMP Agarose Gel-Digesting Preparation .................. 17 Gene Expression Analysis ............................................. 33-35 Gene synthesis ........................................................... 131 GenMap Human BAC Collection ......................................... 28 Genome walking .......................................................... 151 Genomic Cloning ....................................................... 18-21 Genomic DNA, human, sonicated ................................ 149-150 Genomic walking .................................................... 132,151 GFP-anti, antibody ..................................................... 113 GGTase-I ................................................................... 100 GGTase-II .................................................................. 100 Gloves ........................................................................ 75 Glucagon-like peptides ................................................. 130 Glucocorticoid receptor ............................................... 123 Glutathione S-transferase ............................................. 126 Glycogen synthase, substrate for CamK II ......................... 109 Glycogen synthase, substrate for PKC .............................. 109 Glycosidase assay- substrates .......................................... 88 Glycosylase, HK-UNG Uracil-DNA ..................................... 41 Glycosylases & Excision Mixes .......................................... 41 Glycosylases ................................................................. 41 GNDF, Glial-derived neurotrophic factor .......................... 125 GPCRs (G-protein coupled receptors) ........................ 100-103 G-protein coupled receptors- cell extract ........................ 103 G-protein ................................................................... 99 Gram Positive DNA Purification Kit, MasterPure ................. 2 growth factors ........................................................... 125 Growth Hormone releasing factor ................................... 130 Growth Hormone ........................................................ 125 Growth Hormone-20K ................................................... 125 GST ......................................................................... 126 GST-anti, antibody ...................................................... 113
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INDEX
GTP (Guanosine-5'-Triphosphate) ..................................... 32 GTP Family ............................................................ 96-103 GTP Solution ................................................................ 32 GTP ........................................................................... 32 GTPase Cycle Effectors ............................................ 99-100 GTPase modiying enzyme .............................................. 100 Guanine Nucleotide Exchange Factor .................................. 99 Gustducin ................................................................... 99 H1R (HistamineH1 receptor)+regulator of G-protein signaling 102 H1R (HistamineH1 receptor) .......................................... 102 H1R-G protein fusion protein ......................................... 102 H2 R(Histamine H2 receptor) ......................................... 101 H2R (HistamineH2 receptor)+regulator of G-protein signaling ................................................ 102 H2R-G protein fusion protein ......................................... 102 HA-anti, antibody ....................................................... 113 Halogen containing nucleotides ......................................... 80 Halogen-containing ATP analogs ......................................... 80 Halogen-containing GTP analogs ........................................ 80 HA-Tagged Yeast Strains ................................................. 27 HBTU ....................................................................... 128 Hck SH3 domain .......................................................... 106 HCV Protease assay kits86 HCV Protease- substrates ............................................... 86 heavy atom derivatization -crystallography ........................ 80 Hela cell cytosol extract ................................................ 123 Hela cell nuclear extracts .............................................. 123 Hepatitis A proteins ..................................................... 117 Hepatitis B proteins .................................................... 117 Hepatitis C proteins ............................................... 117-119 Hepatitis C-related peptides ......................................... 130 Hepatitis D proteins .................................................... 119 Hepatits E proteins ..................................................... 119 Herpes proteins .......................................................... 117 HEX ......................................................................... 135 HGF, Hepatocyte Growth factor ..................................... 125 High Fidelity Polymerase .................................................. 9 High Range DNA Ladder .................................................. 11 Hilyte Fluor ................................................................ 135 HisTag-anti, antibody .................................................. 113 Histamine receptors .................................................... 102 HIV Protease - substrates ............................................... 86 HIV Protease assay kits .................................................. 86 HIV related peptides ................................................... 130 HIV Reverse Transcriptase .............................................. 15 HIV-1 proteins ...................................................... 119,120 HIV-2 proteins ........................................................... 120 HK Thermolabile Phosphatase ........................................ 42 HK-UNG Thermolabile Uracil N-Glycosylase ........................ 41 HMG1, High mobility group .......................................... 1122 HMP linker ................................................................ 128 HOAt ....................................................................... 128 HOBt ....................................................................... 128 Hoechst dyes .............................................................. 137 Holoenzyme, E coli RNA Polymerase, (sigma-saturated) .......... 29 Homogenizer ................................................................ 67 Hot Start Polymerase ...................................................... 9 H-Ras ......................................................................... 96 Human ORF Collection .................................................... 25 Human T-cell Lymphotropic proteins (HTLV) ...................... 120 Hybridase Thermostable RNase H ................................... 39 Hybridization bottles ..................................................... 72 Hybridization Oven ................................................... 72-73 Hydrogen Peroxidase assay kit ......................................... 91 HyperMu <CHL-1> Forward Primer .................................. 55 HyperMu <CHL-1> Insertion Kit ....................................... 55 HyperMu <CHL-1> Reverse Primer .................................. 55 HyperMu <CHL-1> Transposon ........................................ 55 HyperMu <KAN-1> Insertion kit ...................................... 55 HyperMu <KAN-1> Transposon ........................................ 55 HyperMu <R6K ori/KAN-1> Tnp Transposome Kit .............. 52 HyperMu <R6K ori/KAN-1> Transposon ............................. 52 HyperMu Transposase .................................................. 50 IBI Base Runner 100 & 200 sequencers ............................... 62 IBI MP1015 Electrophoresis System ................................... 60 IBI STS-45I Sequencer .................................................... 62 IBI Ultra-wide mini gel submarine system ........................... 60 IBI Universal Protein Gel systems ...................................... 61 IFN- , 2b .................................................................. 124 IFN- -1b ................................................................... 124 IFN- -2a ................................................................... 124 IFN- ........................................................................................................ 124 IFN- ........................................................................................................ 124 IL-1 receptor antagonist ............................................... 125 IL-10 ........................................................................ 124 IL-11 ........................................................................ 124 IL-15 ........................................................................ 125 IL-1a ........................................................................ 125 IL-1b ....................................................................... 125 IL-2 ......................................................................... 125 IL-3 ......................................................................... 124 IL-4 ......................................................................... 125 IL-5 ......................................................................... 125 IL-6 ......................................................................... 125 IL-7 ......................................................................... 125 IL-9 ......................................................................... 125 IMAGE ESTs ................................................................. 25 Immobilized 2',3'- EDA Nucleotides .................................... 58 Immobilized 8-Aminobutyl Nucleotides ............................... 58 Immobilized 8-Aminohexyl Nucleotides ............................... 58 Immobilized Tyrosines .................................................... 58 Importin ................................................................... 103 Incyte Gene Collection .................................................... 25 Indo-1 ...................................................................... 143 Indo-5F .................................................................... 143 Induction Solution, CopyControl .................................. 18,19 Induction Solution, CopyCutter ....................................... 24 Inhibitor of DNA Cleavage, TypeOne Restriction Inhibitor ..... 17 Inhibitor of Transcription, Tagetin RNA Polymerase Inhibitor ................................................................ 32 Inhibitor Remover Resin .................................................. 2 Inhibitors .......................................... (see relevant section) Inserting a Promoter ..................................................... 49 Inserting an Origin of Replication ..................................... 49 Insertion Kits ............................................................... 49 Insulin like Growth factor binding protein ................... 125,126 Insulin like Growth factor ........................................ 125,126 Insulin ...................................................................... 124 In-vitro Transcription ................................................ 29-32 Isofreeze flipper temperature maintenance racks ................ 66 JBS DNA Shuffling Mutagenesis Kit ..................................... 54 JBS dNTP Mutagenesis Kit ............................................... 54 JBS Error-Prone Random Mutagenesis Kit ........................... 54 JBS Halo kits ................................................................ 80 JBS Halo-ATP kits .......................................................... 80 JBS Halo-GTP kits ......................................................... 80 JBS Membrane Screen ................................................... 77 JBS Methylation kit ....................................................... 79 JBS Solubility kits ......................................................... 79 JBScreen Basic ............................................................. 78 JBScreen Buffer kits ..................................................... 78 JBScreen Bulk kits ......................................................... 77 JBScreen Cryo ............................................................. 82 JBScreen Crystal Screening Kits ....................................... 77 JBScreen Detergents ..................................................... 79 JBScreen Heavy ............................................................ 80 JBScreen HTS ............................................................... 77 JBScreen Plus .............................................................. 79 JBScreen Refill kits ....................................................... 77 JBSolution Detergent test kit ........................................... 79 JNK2a/SAPK1a (c-jun kinase/stress-activated protein kinase) 108 JOE ......................................................................... 136 KAN-2 FP-1 Forward Sequencing Primer ............................ 55 KAN-2 RP-1 Reverse Sequencing Primer ............................. 55 Kemptide, substrate for PKA ........................................ 108 KGF-1, keratinocyte growth factor-1 .............................. 125 KGF-2, keratinocyte growth factor-2 .............................. 125 Klenow DNA Polymerase ................................................. 7,8 KLH, maleimidyl .......................................................... 115 Kool NC-45 RNAP Activity & Inhibitor Screening Kit ............... 30 Kool NC-45 Universal RNA Polymerase Template .................... 30 K-Ras ......................................................................... 96 Labels ........................................................................ 75 Ladders .................................................................. 11,12 Lambda DNA (Bgl I) ....................................................... 11 Lambda DNA/Bme (Ava II) ............................................... 11 Lambda DNA/Bss T1I (StyI) ............................................. 11 Lambda DNA/HindIII ...................................................... 11 Lambda DNA ................................................................ 12 Lambda Exonuclease ...................................................... 38 Lambda GT10 Forward DNA Sequencing Primer .................... 13
iv
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INDEX
Lambda GT10 Reverse DNA Sequencing Primer ..................... 13 Lambda GT11 Forward DNA Sequencing Primer .................... 13 Lambda GT11 Reverse DNA Sequencing Primer ..................... 13 Lambda Terminase ........................................................ 38 Lck SH3 domain .......................................................... 106 Leptin, obesity factor ................................................. 124 Leukemia Inhibitory factor ........................................... 124 Leukotriene receptors ................................................. 102 Leutenizing Hormone ................................................... 130 LEXSY, Leishmania Expression System .......................... 153-154 Library construction ................................................... 131 Ligase, T4 DNA, Cloned .................................................. 43 Lipophilic membrane tracers .................................... 145-148 Liquid nitrogen dewars .................................................. 65 Liver-X receptor ........................................................ 123 Log Scale DNA Ladder .................................................... 12 Long Range Polymerase .................................................... 9 Low Range DNA Ladder ................................................... 11 Luciferin ..................................................................... 92 LY294002 ................................................................... 105 Lysozyme .................................................................... 57 M13 forward and reverse primers .................................... 13 M13/pUC Forward DNA Sequencing Primer ......................... 13 M13/pUC Reverse DNA Sequencing Primer .......................... 13 Magnesium Chloride ....................................................... 12 magnetic stirrer with hot plate ........................................ 68 magnetic stirrer .......................................................... 68 Malaria aspartyl Proteinase FRET substrate ........................ 87 Mammalian Gene Collection .............................................. 25 MAPK2 (mitogen activated kinase) .................................. 108 MARKS Protein, substrate for PKC ................................. 109 MasterAmp 10X PCR Enhancer ........................................ 32 MasterAmp AmpliThem DNA Polymerase ........................... 8 MasterAmp Buccal Swab Brushes - Remote Site Testing ......... 1 MasterAmp Buccal Swab Brushes ..................................... 1 MasterAmp Buccal Swab Kit - Remote Site Testing ............... 1 MasterAmp Buccal Swab Kit ........................................... 1 MasterAmp DNA Extraction Solution .................................. 1 MasterAmp Extra-Long 2X PCR Premix 1 to Premix 9 ........... 6 MasterAmp Extra-Long DNA Polymerase Mix ....................... 6 MasterAmp Extra-Long PCR Kit ....................................... 6 MasterAmp GREEN Real-Time RT-PCR Kit .......................... 10 MasterAmp High Fidelity RT-PCR Kit ............................... 14 MasterAmp PCR Optimization Kit with (NH4)2SO4 ............. 5-6 MasterAmp PCR Optimization Kit without (NH4)2SO4 ......... 5-6 MasterAmp RT-PCR Kit for High Sensitivity ....................... 15 MasterAmp Taq DNA Polymerase Kit .............................. 7,8 MasterAmp Taq DNA Polymerase .................................... 7,8 MasterAmp Taq PCR Core Kit ......................................... 8 MasterAmp Tfl DNA Polymerase Kit ................................ 7,8 MasterAmp Tfl DNA Polymerase ..................................... 7,8 MasterAmp Tth DNA Polymerase Kit ................................ 7,8 MasterAmp Tth DNA Polymerase .................................... 7,8 MasterPure Complete DNA & RNA Purification Kit ............... 3 MasterPure DNA Purification Kit ..................................... 3 MasterPure DNA Purification Kit for Blood ......................... 2 MasterPure Gram Positive DNA Purification kit ................... 2 MasterPure Plant Leaf DNA Purification Kit ........................ 2 MasterPure Precipitation Solution for Blood ....................... 3 MasterPure RNA Purification Kit ..................................... 3 MasterPure Yeast DNA Purification Kit .............................. 4 MasterPure Yeast RNA Purification Kit .............................. 4 Matrix Metalloproteinases ........................................... 83-85 Matrix Metalloproteinases-Substrates FRET based ............ 83-85 MaxPlax Lambda Packaging Extract ................................. 21 M-CSF ...................................................................... 124 MEK1 ....................................................................... 106 Membrane potential probes ...................................... 145-148 Mesophilic DNA Polymerases ........................................... 7-9 MessageMAX T7 Capped Message Transcription Kit ............... 31 MessageMuter shRNAi Production Kit ............................... 37 MgCI2, 25 mM .............................................................. 58 MIA, Melanoma Inhibitory activity protein ........................ 126 Microcentrifuge ........................................................... 64 Microfuge ................................................................... 64 Mid Range DNA Ladder ................................................... 11 Minifridge .................................................................. 66 Minifuge ..................................................................... 64 MiniV In vitro Transcription kit ....................................... 30 MKK6 ....................................................................... 106 MMLV Reverse Transcriptase ........................................... 14 MMP- antibodies .......................................................... 85 MMP-1 Assay kits ...................................................... 83,84 Monoclonal antibody production ..................................... 133 Monomethylated; m7G[5]ppp[5]G .................................. 19 MonsterScript 1st-strand cDNA synthesis kit ....................... 14 MonsterScript Reverse Transcriptase ................................ 14 Mouse BAC Clones ......................................................... 28 MPC Protein Precipitation Solution .................................... 3 MPC Protein Precipitation Solution ................... 215 M-Ras ........................................................................ 96 mRNA Amplification ................................................... 33-35 mRNA Purification .......................................................... 3 mRNA Purification .......................................................... 3 mRNA-ONLY Eukaryotic mRNA Isolation kit ............................ 3 mRNA-ONLY Prokaryotic mRNA Isolation kit .......................... 3 Mthylene blue ............................................................. 137 Mu-EndProtein Truncation Kit ....................................... 53 MUKAN-1 FP-1 Forward Sequencing Primer ......................... 52 MUKAN-1 RP-1 Reverse Sequencing Primer ......................... 52 Multidrug resistance assays ........................................... 133 Mung Bean Nuclease ....................................................... 40 Mutagenesis Kits ........................................................... 54 Mutation Detection ........................................................ 43 Myosin II, non muscle, regulatory ................................... 126 Myricetin .................................................................. 105 Natriuretic peptides ................................................... 130 NBT/ROCK ................................................................ 113 Needles and Loops for Inoculation ..................................... 76 Nerokinins ................................................................. 130 Nerve Growth factor ................................................... 126 Neurogranin, substrate for PKC ..................................... 109 Neuromedins .............................................................. 130 Neuropeptide Y and analogs ........................................... 130 Neurotensins and related peptides .................................. 130 Neurotrophin3 ........................................................... 126 Nickase ...................................................................... 41 Nile Red .................................................................... 141 Ninhydrin test kit ....................................................... 128 Nitrocellulose membranes ................................................ 63 Not I/KAN-3 FP-2 Forward Sequencing Primer .................... 54 Not I/KAN-3 RP-2 Reverse Sequencing Primer ..................... 54 N-Ras ......................................................................... 96 NTP Mixture (ATP, CTP, GTP, UTP) ................................... 32 NTPhos Thermolabile Phosphatase ..................................... 42 Nuclear receptors ....................................................... 123 Nuclease S1 .................................................................. 41 Nylon membranes .......................................................... 63 Oil Red ..................................................................... 141 Oligo dT ..................................................................... 13 Oligo modifcation ......................................................... 13 Oligo Synthesis ............................................................. 13 OmniCleave Endonuclease .............................................. 40 OPD ........................................................................... 91 Optimization Kit ........................................................... 6 Orexins .................................................................... 130 Osteocalcins .............................................................. 130 Owl adjustable Vertical system ........................................ 60 Owl Bandit Mini Tank electroblotter .................................. 63 Owl Bandit Tank Electroblotting sytems .............................. 63 Owl Buffer Puffer Self ReCirculating Gel System .................. 59 Owl Centipede Minigel System .......................................... 59 Owl Gator Gel System .................................................... 59 Owl Millipede Horizontal System ....................................... 60 Owl Otter sequencing gel casting system ............................ 62 Owl Panther Semi Dry Electroblotters ................................ 62 Owl Penguin Dual-gel water-cooled electrophoresis system ...... 61 Owl Puffin Single sided Minigel system ............................... 60 Owl simple cast Minigel Systems ....................................... 59 Owl Spider Electrophoresis System ................................... 59 Owl Submarine Gel Electrophoresis equipment ..................... 59 Owl T-Rex Aluminium Backed Sequencing System ................... 62 Owl Whirlsystem Recirculating Gel system ........................... 59 Oxidase assay substrates ................................................ 92 Oxytocins .................................................................. 130 Ozma PEG-Series 48-Salt ................................................ 78 p300, tumor suppressor gene and transcription factor ........ p38a/SAPK2 ............................................................. p42 ......................................................................... p44 ......................................................................... p52, transcriptional coactivator .................................... p53, C-term deletion ................................................... p53, wild type ........................................................... 122 108 108 108 122 122 122
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INDEX
p75, transcriptional coactivator .................................... 122 p85 ........................................................................ 104 Pancreatic polypeptides ............................................... 130 Parathyroid hormone and related peptides ....................... 130 pBR322 DNA ................................................................ 12 PC (Phospatidylcholine) ................................................ 105 PC4, positive cofactor 4 ............................................... 122 pCC1BAC/plndigoBAC-5 Forward Sequencing Primer ....... 19,20 pCC1BAC/plndigoBAC-5 Reverse Sequencing Primer ........ 19,20 pCC1FOS/pEpiFOS-5 Forward Sequencing .................... 18,19 pCC1FOS/pEpiFOS-5 Reverse Sequencing Primer .......... 18,19 pCC2 Forward Sequencing Primer .................................... 18 pCC2 Reverse Sequencing Primer ..................................... 18 PCR CleanUp ................................................................ 17 PCR Cloning ............................................................. 16,17 PCR Enhancer ............................................................... 12 PCR Optimization .......................................................... 6 PCR ........................................................................ 5-17 PDGFR (Platelet-derived growth factor receptor) .............. 106 PE (Phosphatidylethanolamine) ....................................... 105 Peptide synthesis ........................................................ 132 Peptide YY and analog .................................................. 130 Periplasmic protein extraction ........................................ 57 PeriPreps Periplastic Protien Extraction Kit .................... 57 peroxidase assay - substrates .......................................... 91 Phage Lambda Extracts .................................................. 21 Phage T7 DNA ............................................................... 12 Phi29 DNA Polymerase, RepliPHI ...................................... 9 Phi29 Polymerase Dilution Buffer, RepliPHI ......................... 9 Phi29 Reagent Set, RepliPHI ............................................ 9 Phosphatase assay kits ............................................ 111,112 Phosphatase assay- substrate ........................................... 89 Phospholipase assay- substrate ........................................ 89 Phosphopeptides ........................................................ 130 PI (Phosphatidylinositol) ............................................... 105 PI3K antibodies .......................................................... 105 PI3K inhibitors ........................................................... 105 PI3K lipid kinase mix1 .................................................. 105 PI3K lipid kinase mix2 .................................................. 105 PI3K ....................................................................... 104 PI3K ....................................................................... 104 PI3K ....................................................................... 105 PI3K ....................................................................... 104 PI-4,5P2 (Phosphatidylinositol bisphosphate) ..................... 105 PI-4P ....................................................................... 105 pIndigoBAC-5 (BamH I Cloning-Ready) ............................... 20 pIndigoBAC-5 (Hind III Cloning-Ready) .............................. 20 pIndigoBAC-5 Sequencing Primers .................................... 20 Piperidine ................................................................ 128 Pituitary adenylate cyclase activating peptides ................. 130 Pituitary Growth hormone ............................................ 125 PKA (Protein kinase A) ................................................. 106 PKC ....................................................................... 106 Plasmid DNA library construction ................................... 132 Plasmid prep ............................................................... 16 PlasmidMAX Plasmid Isolation kit ...................................... 16 Plasmid-Safe ATP-Dep DNase .......................................... 16 Platelet derived growth factor ...................................... 126 Platelet-activating factor receptors ................................ 103 Pluronic F-127 ............................................................ 144 pMOD<MCS> Forward PCR Primer .................................. 51 pMOD<MCS> Forward Sequencing Primer ......................... 51 pMOD<MCS> Reverse PCR Primer ................................... 51 pMOD<MCS> Reverse Sequencing Primer .......................... 51 pNPP ................................................................... 89, 113 Poly(A) Tailing .............................................................. 31 Polyclonal antibody production ...................................... 133 Ponceau .................................................................... 141 Power supplies ............................................................. 61 Power supply for sequencing gel ....................................... 61 PPAR, Peroxisome Proliferator-activated receptor ............ 123 pPDM Sequencing Primer for pPDM-1 & pPDM-2 ........... 54 pPDM-1 Cloning Plasmid ............................................... 54 pPDM-2 Cloning Plasmid ................................................ 54 Precipitation Solution for Blood ........................................ 3 PreMix-Choice Kits ........................................................ 6 Pre-mRNA splicing factors ............................................ 122 Primed in situ synthesis .......................................... 149-150 Primers Commonly Used for Sequencing ............................. 13 Primers ...................................................................... 13 Prion associated proteins ............................................. 116 Prion cDNA ................................................................ 116 Prion proteins ........................................................... 116 PRL-3 ....................................................................... 111 Prolactin releasing peptides .......................................... 130 Prolactin ................................................................... 124 Propidium iodide ....................................................... 137 Protease assay kits ....................................................... 87 Protease assay- substrates .............................................. 87 Proteases .................................................................... 87 Protein kinase related peptides ..................................... 130 Protein kinase substrate sampler kit ............................... 112 Protein labeling kit, 5-FAM ............................................ 142 Protein labeling kit, 5-ROX ........................................... 142 Protein labeling kit, 5-TAMRA ........................................ 142 Protein labeling kit, Alkalin phosphatase ........................... 142 Protein labeling kit, AMCA-X ......................................... 142 Protein labeling kit, APC ............................................... 142 Protein labeling kit, Biotin ............................................ 142 Protein labeling kit, Hilyte Fluor ..................................... 142 Protein labeling kit, HRP .............................................. 142 Protein labeling kit, Phycoerythrin ................................. 142 Protein labeling kit, TR ................................................ 142 Protein Molecular weight Marker ...................................... 12 Protein Quantitation kit1 ................................................ 41 Proteinase K ................................................................. 3 Proteolipid proteins (PLPs) ........................................... 130 PS (Phosphatidylserine) ............................................... 105 PTEN ....................................................................... 111 PTP-1B .................................................................... 111 PTP-Basophil like ........................................................ 111 PTPm ....................................................................... 111 PTP-SL-catalytic domain .............................................. 111 PTP-SL-ex ................................................................. 111 PTP-SL-kim (kinase interacting motif) ............................. 111 pUC 19 DNA ................................................................. 12 pUC 19/MspI ................................................................ 11 pUC19 DNA Control Template ........................................... 11 PVDF Membranes .......................................................... 63 pWEB::TNC Cosmid Cloning Kit ....................................... 21 pWEB::TNC Cosmid Vector ............................................ 21 pWEB::TNC Deletion Cosmid Transposition Kit ................... 21 pWEB Cosmid Cloning Kit .............................................. 21 Pyronin Y .................................................................. 137
Q
Q-Beta Replicase .......................................................... 29 Quantitative PCR and RT-PCR Quenchers ............................................................ 139,140 Quercetin ................................................................. 105 QuickExtract DNA Extraction Solution ................................ 1 QXL .................................................................... 139,140 R6KAN-2 Reverse Primer ................................................ 52 Rab GGTase ............................................................... 100 Rab17 ........................................................................ 97 Rab1A ........................................................................ 97 Rab1B ........................................................................ 97 Rab2 .......................................................................... 97 Rab24 ........................................................................ 97 Rab27A ...................................................................... 97 Rab3A ........................................................................ 97 Rab4A ........................................................................ 97 Rab6A ........................................................................ 97 Rab7 .......................................................................... 97 RabGDI ...................................................................... 97 Rac1 .......................................................................... 98 RAD51 ...................................................................... 122 RafRBD ................................................................ 99-100 RalGDS-RBD ................................................................. 99 Ran ........................................................................... 98 Random primers ........................................................... 13 RanGAP ...................................................................... 99 Rap1A ........................................................................ 96 Rap1B ........................................................................ 96 RAP30 ...................................................................... 121 RAP74 ...................................................................... 121 RapGAP ...................................................................... 99 Ras ........................................................................... 96 RasGAP ...................................................................... 99 RB, retinoblastoma protein ........................................... 122 rBst DNA Polymerase ...................................................... 7 Ready-Lyse Lysozyme Solution ........................................ 57 ReadyPreps Protein Preparation Kit ............................... 57 Real-Time PCR ............................................................. 10 Real-Time RT-PCR ......................................................... 15 Rec A Protein ............................................................... 37
vi
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Rec J Exonuclease ......................................................... 39 RecA Protein, E coli ....................................................... 37 RecBCD Nuclease, Ecoli ................................................... 39 Red Cell Lysis Solution ..................................................... 3 Renin-substrate ............................................................ 87 REP-1 ....................................................................... 100 REP-2 ....................................................................... 100 Replica plating device .................................................... 76 RepliPHI Phi29 DNA Polymerase ........................................ 9 Rescue Cloning of Insertion Mutations ................................ 50 resins, 2-chlorotrityl ................................................... 128 resins, AFC, AMC, pNA ................................................. 128 resins, Fmoc-Rink amide .............................................. 128 resins, HMP ............................................................... 128 resins, MAPS ............................................................. 128 resins, MBHA ............................................................. 128 resins, merrifield ....................................................... 128 resins, PAM ............................................................... 128 resins, TentaGel ......................................................... 128 resins, Wang .............................................................. 128 resins ...................................................................... 128 Resorufin .................................................................... 89 Restriction enzymes .................................................. 44-48 Retinoic acid receptor ................................................. 123 RetroAmp RT DNA Polymerase ........................................ 15 Retroviral vectors ......................................................... 36 Reverse transcriptase .................................................... 14 RhoA .......................................................................... 98 Rhod-2 ................................................................ 143,144 Rhod-5F ............................................................... 143,144 Rhod-5N .............................................................. 143,144 Rhodamine ................................................................ 136 Rhotekin ............................................................... 99-100 Ribonucleoside-5-Triphosphate Solutions ........................... 32 RiboScribe SP6 RNA Probe Synthesis Kit ........................... 30 RiboScribe T3 RNA Probe Synthesis Kit ............................ 30 RiboScribe T7 RNA Probe Synthesis Kit ............................ 30 RiboShredder RNase Blend ............................................ 40 RIN2-RA .................................................................... 100 Rink amide linker ........................................................ 128 RNA Cap Analog, Methylated ............................................ 31 RNA Cap Analog, Trimethylated ........................................ 31 RNA Cap Analog, Unmethylated ......................................... 31 RNA Interference (RNAi) ............................................. 36-37 RNA Labeling ............................................................ 33-35 RNA pol II- hRPB10 ...................................................... 121 RNA pol II- hRPB12 ...................................................... 121 RNA pol II- hRPB5 ........................................................ 121 RNA pol II- hRPB6 ........................................................ 121 RNA pol II- hRPB9 ........................................................ 121 RNA pol II .................................................................. 121 RNA pol II-CTD ........................................................... 121 RNA Polymerases ........................................................... 29 RNA Probe Synthesis ...................................................... 30 RNA Purification for Microarray ....................................... 4 RNA Purification from Yeast ............................................. 4 RNA Purification .......................................................... 3,4 RNAi ...................................................................... 36-37 RNase A ...................................................................... 39 RNase A-Resistant RNA ................................................... 30 RNase H, E coli ............................................................. 39 RNase I, E coli .............................................................. 40 RNase III, E coli ............................................................ 40 RNase T1, Aspergillus oryzae ........................................... 40 RNase TA ..................................................................... 41 RNase-Free DNase I ....................................................... 38 RNTP ......................................................................... 32 rocker- 3D .................................................................. 69 rocker ....................................................................... 68 Rolling Circle Transcription ............................................. 32 ROX ......................................................................... 136 RT-PCR .................................................................. 14,15 Rubella virus proteins .................................................. 120 S100A, Calcium binding protein A ................................... 126 SARS virus proteins ..................................................... 120 SB202190 (p38 MAPK inhibitor) ...................................... 108 SB203580 (p38 MAPK inhibitor) ...................................... 108 SCF, Stem cell factor .................................................... 124 Secretins .................................................................. 130 Sequencing .............................................................. 55,56 Sequencing Polymerase .................................................... 9 Sequencing Primer-1/pWEB::TNC Cosmid Vector ............... 21 SequiTherm Cycle Sequencing Kit .................................... 56 SequiTherm DNA Polymerase .......................................... 56 SequiTherm EXCEL II DNA Sequencing Kit - LC for 25-41 cm gels ..................................................... 56 SequiTherm EXCEL II DNA Sequencing Kit - LC for 66 cm gels 56 SequiTherm EXCEL II Long-Read DNA Sequencing Kit - ALF 56 SequiTherm EXCEL II Manual Sequencing Kit ..................... 56 SequiTherm Stop/Gel Loading Buffer (Automated) .............. 56 SequiTherm Stop/Gel Loading Buffer (Manual) ................... 56 SequiTherm Stop/Gel Loading Buffer for LI-COR or NEN .. 56 serine/threonine kinase substrate sampler kit ................... 112 Serine/Threonine kinases ......................................... 106-110 shaker orbital .............................................................. 69 shaker ........................................................................ 69 Short-Hairpin RNA .................................................... 36-37 SHP-1 (src homology 2 domain phosphatase) ...................... 111 shRNAi controls ............................................................ 36 shRNAi Libraries .......................................................... 36 shRNAi transfection kits ................................................. 36 Signal Transduction blocking peptides .............................. 130 Single Stranded DNA Binding Protein ................................. 37 Site directed mutageneis .............................................. 132 Slides for Microarray .................................................... 76 Slot-Blot Manifold systems .............................................. 63 Slot-Blot Minifold systems ............................................... 63 SoilMaster DNA Extraction Kit ......................................... 2 SoilMaster Inhibitor Removal Resin ................................... 2 SoilMaster Spin Columns ................................................. 2 Solubility kits ............................................................... 79 Somatostatins ............................................................ 130 Sp1 ......................................................................... 122 SP6 Promoter DNA Sequencing Primer ............................... 13 SP6- Promoter Primer ................................................... 13 SP6 R&DNA Polymerase ................................................. 29 SP6 RNA Polymerase ...................................................... 29 SP600125 (JNK inhibitor) .............................................. 108 Sphingomyelin ............................................................ 105 Spin Columns Spreader .................................................................... 76 Src SH3 domain .......................................................... 106 S r c ......................................................................... 106 Stains-all ................................................................... 137 Staurosporine ............................................................ 105 Stickleback cDNA Collection ............................................. 26 stirrer ....................................................................... 68 Streptavidin, alkaline phosphatase .................................. 115 Streptavidin, fluorophore conjugated ............................. 115 Streptavidin, HRP ....................................................... 115 Streptavidin .............................................................. 115 Subcloning ................................................................. 132 Substance P and analogs ............................................... 130 Substrate for Calcineurin .............................................. 110 Substrate for casein kinase ........................................... 110 substrate for cdk ...................................................... 1110 Substrate for cdk ...................................................... 5110 Substrate for MAPK .................................................... 110 Substrate for p43 (CD2) ............................................... 110 Substrate for p60-src .................................................. 110 Substrate for PKCe ..................................................... 109 Substrate for PKCm .................................................... 109 Substrate for PKG ...................................................... 109 Substrate peptide for AMPK ......................................... 108 Substrate peptide for CHK ............................................ 108 Substrate peptide for CK1 ............................................ 108 Substrate peptide for CK2 ............................................ 108 Substrate peptide for DYRK .......................................... 108 Substrate peptide for GSK3 .......................................... 108 Substrate peptide for LKB1 .......................................... 108 Substrate peptide for PKA ............................................ 108 Substrate peptide for PKC ............................................ 108 Substrate peptide for src ............................................. 108 Substrate peptides for Abl ............................................ 108 Substrate peptides for Akt ........................................... 108 Substrate peptides for Cdc-2 ........................................ 108 Substrates for CaM Kinase II ......................................... 109 Substrates for PTK ..................................................... 110 Substrates for PTP ..................................................... 110 Substrates for PTP-1B ................................................. 110 Sulfoxide reductase, methionine ..................................... 126 Supercoiled DNA Marker Set ............................................ 20 Supercoiled DNA Marker Set ............................................ 20 Superoxide dismutase .................................................. 126 Swabs, CatchAll Sample Collection Swabs ............................ 1 Syntide, substrate for CamKII ....................................... 109
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vii
INDEX T
Transformation & Storage Solution .................................... 17 Transformation ........................................................ 17,23 TransforMax EC100D pir+ Electrocompent E coli ........... 23,24 TransforMax EC100D pir-116 Electrocompent E coli ....... 23,24 TransforMax EC100 Chemically Competent E coli .......... 23,24 TransforMax EC100 Electrocompetent E coli ................. 23,24 TransforMax EC100-T1R Chemically Competent E coli ................................................. 23,24 TransforMax EC100-T1R Electrocompetent E coli ........... 23,24 TransforMax EPI300 Chemically Competent E coli ......... 23,24 TransforMax EPI300 Electrocompetent E coli ................ 23,24 TransforMax EPI300-T1R Chemically Competent E coli ... 23,24 TransforMax EPI300-T1R Electrocompetent E coli .......... 23,24 Transilluminators .......................................................... 74 Transposome kits .......................................................... 52 Transposon Construction Vector ....................................... 51 Transposon Construction Vectors ...................................... 51 Transposons ............................................................. 49-55 Treponema proteins .................................................... 120 Trimethylated; m2,2,7G[5]ppp[5]G ............................... 31 TRITC ...................................................................... 137 Trypsin-substrate ......................................................... 87 TypeOne Restriction Inhibitor ........................................ 17 Tyrosine Hydroxylase, substrate for MAPK ....................... 110 Tyrosine kinases ......................................................... 106 Tyrosine like kinases .................................................... 106 Tyrosine protein kinase substrate sampler kit ................... 112 Universal Primers ......................................................... 13 Unmethylated; G[5]ppp[5]G ......................................... 31 Uracil N-Glycosylase , HK-UNG ........................................ 41 Uracil-DNA Excision Mix .................................................. 41 Urea peroxide tablets .................................................. 113 UTP (Uridine-5'-Triphosphate) ......................................... 32 UTP (Uridine-5'-Triphosphate) ......................................... 32 UV Crosslinker ............................................................. 74 UV Lamp- Handheld ....................................................... 74 UV shield/goggles .......................................................... 76 UV-White ligh plate converter .......................................... 74 Vaccinia DNA Topoisomerase I .......................................... 37 Vasoactive Intestinal peptides ........................................ 130 Vasopressins .............................................................. 130 VEGF, Vascular Endothelial growth factor ......................... 126 Ventricular Myosin regulatory light chain .......................... 126 VH1-related phosphatase .............................................. 111 Vortex mixer ............................................................... 68 VP16, Herpes simplex virion trans-activating protein ......... 121 Varizella-Zoster Virus proteins ...................................... 120 WASP-121 ................................................................. 100 water bath .................................................................. 70 Water, Sterile Nuclease-Free ........................................... 12 Water ........................................................................ 12 waterbath- shaking ....................................................... 70 waterbath-circulating .................................................... 71 WaterMaster DNA Purification kit ...................................... 2 Wizard I and II ............................................................. 78 Wortmannin .............................................................. 105 WT-1(+KTS), Wilms tumor suppressor and transcription splicing factor .................................... 122 WT-1(-KTS), Wilms tumor suppressor and transcription splicing factor ......................................... 122 Xenopus IMAGE cDNA ..................................................... 26 X-Rhod-1 ............................................................. 143,144 X-Y FISH Bovine sex test kit ...................................... 149-150 Yeast Insertional Mutant Strains ....................................... Yeast Knockout Strains ................................................... Yeast ORF Collection ...................................................... Yeast TAP Fusion Collection .............................................. Yeast Tet-promoter Hughes Strains .................................... 27 27 27 28 28
T3 Promoter DNA Sequencing Primer ................................. 13 T3- Promoter primer .................................................... 13 T3 RNA Polymerase ........................................................ 29 T4 DNA Endonuclease V ................................................... 38 T4 DNA Ligase, Cloned .................................................... 43 T4 DNA Polymerase ......................................................... 9 T4 Polynucleotide Kinase, Cloned ...................................... 43 T4 RNA Ligase .............................................................. 43 T7 DNA Polymerase, Unmodified ........................................ 9 T7 Promoter DNA Sequencing Primer ................................. 13 T7- Promoter primer .................................................... 13 T7 R&DNA Polymerase .................................................. 29 T7 RNA Polymerase ........................................................ 29 TA Buffer 10X .............................................................. 12 TACE-substrate ............................................................ 87 Tagetin RNA Polymerase Inhibitor ................................... 32 TAMRA ..................................................................... 136 Taq DNA Polymerase, MasterAmp ..................................... 8 TAQurate GREEN Real-Time PCR MasterMix ....................... 10 TAQurate PROBES Real-Time PCR MasterMix ..................... 10 TargetAmp 1-Round amplification kit 103 ...................... 33-35 TargetAmp 1-Round Aminoallyl-aRNA amplification kit 101 .. 33-35 TargetAmp 2-Round Aminoallyl-aRNA amplificcation kit 10 .. 33-35 TargetAmp 2-Round Aminoallyl-aRNA amplificcation kit 20 .. 33-35 TATA box binding protein .............................................. 121 Taylor-Wharton Cryogenic Refrigerators ............................ 65 TBTU ....................................................................... 128 TC-21/R-Ras 3 ............................................................. 96 TC21-R-Ras 2 ............................................................... 96 TE Buffer ..................................................................... 3 Terminator 5'- Phosphate-Dependent Exonuclease ................. 40 TET ......................................................................... 137 TET-1 FP-1 Forward Primer ............................................ 55 TET-1 RP-1 Reverse Primer ............................................. 55 TFIIA ....................................................................... 121 TFIIA-p12 .................................................................. 121 TFIIA-p55 .................................................................. 121 TFIIB ....................................................................... 121 TFIID ....................................................................... 121 TFIIE ........................................................................ 121 TFIIE , p56 ............................................................... 121 TFIIE , p34 ................................................................ 121 TFIIF, RAP74 .............................................................. 121 TFIIF ........................................................................ 121 TFIIH ....................................................................... 121 TFIIH-p62 .................................................................. 121 Tfl DNA Polymerase, MasterAmp ...................................... 8 Thermolabile Phosphatases .............................................. 42 Thermophilic DNA Polymerases ........................................ 7,8 Thermus Thermostable RNA Polymerase .............................. 29 Thiazole orange .......................................................... 137 Thrombin related peptides ........................................... 130 Thrombopoetin, megakaryocyte colony stimulating factor .... 124 Thyroid hormone receptor ............................................ 123 Thyrotrophin releasing hormones and related peptides ........ 130 Tick-borne Encephalitis protein ..................................... 120 Tissue & Cell Lysis Solution ............................................... 3 tissue tearor ............................................................... 67 Titin ........................................................................ 126 T M B .......................................................................... 91 TN FP-1 Forward Primer ............................................ 49,50 TN RP-1 Reverse Primer ............................................ 49,50 TNF peptides ............................................................. 130 TNF- ...................................................................... 124 TNF- ....................................................................... 124 Tobacco Acid Pyrophosphatase ......................................... 42 Topoisomerase I, core domain ........................................ 122 Topoisomerase I, C-term domain .................................... 122 Topoisomerase I, N-term domain .................................... 122 Topoisomerase I .......................................................... 122 Tough tags, spots .......................................................... 75 Toxins ...................................................................... 130 Toxoplasma proteins .................................................... 120 Trancription factors, activators .................................... 121 Transcription Buffer Package .......................................... 31 Transcription factors, coactivators ................................ 122 Transcription factors ............................................. 121-123
X Y
Zebrafish Gene Collection ............................................... 26 Zebrafish Morpholino Collection ....................................... 26
viii
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Biotechnology Biotechdesk Catalog 2006 07

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Biotechnology Biotechdesk Catalog 2006 07

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A C

DNA/ RNA PURIFICATION

X-RAY CRYSTALLOGRAPHY ........................................ 77

ENZYME DETECTION REAGENTS AND ASSAY KITS ......................................................................... 83

SIGNAL TRANSDUCTION ............................................... 97

cDNA LIBRARIES AND CLONES ................................... 25

ANTIBODIES ..................................................................... 113

IN VITRO TRANSCRIPTION .......................................... 29

PEPTIDE SYNTHESIS ................................................... 127

MODIFYING ENZYMES ................................................... 38

CUSTOM SERVICES ....................................................... 131

FLUORESCENCE ............................................................. 134

CYTOGENETICS .............................................................. 149

PROTEIN PURIFICATION ............................................... 57

NEW TECHNOLOGIES .................................................. 151

INDEX ..................................................................... (i) - (viii)

PlasmidMAX DNA Isolation Kit

MasterPure DNA Purification Kit for Blood

MasterPure Plant Leaf DNA Purification Kit

SoilMaster/ WaterMaster DNA Extraction Kit

MasterPure DNA Purification Kit

MasterPure Yeast DNA Purification Kit

Biotech Desk Pvt. Ltd.

Tel: +91-40-27017477/ 65916167

CUSTOM GENE SYNTHESIS

We guarantee a quick turnaround-time, unbeatable pricing and large inserts!

OTHER GENOMIC SERVICES BAC LIBRARY CONSTRUCTION

Mammalian cDNA Libraries and Clones

Non Mammalian cDNA Libraries and Clones

Yeast cDNA Libraries and Clones

CUSTOM PEPTIDE SYNTHESIS

CUSTOM ANTIBODY PRODUCTION

Additional antibody services include:

Tel: +91-40-27017477/ 65916167

nts a i r a v agged t y t i n i s, Aff n i e t o r p Native

ucts d o r p ciated o s s A &

Protein Kinase A, B and C

Other Related Proteins:

Phosphoinositide 3-kinase (PI3K)

JBScreen Classic bulk

JBScreen Classic ampoules

JBScreen Classic refill

JBScreen Classic HTS

Wizard Screens I & II from Emerald Biosystems

JBScreen Cryo Pro

JBScreen Detergents kits

JBS Halo Kits

ANTIBODIES FOR EVERYTHING!!

Epitope Tagged Antibodies

Antibody Related Kits:

CopyControl BAC Library Production

Maximise yields from every clone!!

Biotech Desk Pvt. Ltd.

Optimizing Real-Time PCR

Using SYBR Green I Dye

And EVERY TIME

MasterAmp GREEN Real-Time RT-PCR Kit

MasterAmp High Sensitivity RT-PCR Kit

MasterAmp High Fidelity RT-PCR Kit

Highly accurate RT-PCR amplification of RNA templates for

Cloning Sequencing Expression Transcription analysis

Biotech Desk Pvt. Ltd.

Tel: +91-40-27017477/ 65916167