CN2108 Data Sheet

CI. Protein Quantification

-amylase
Sample
S25
S60

Concentration (ppm)
196.069
166.447

Retention Time (min)

1.350
1.347

CII. Protein Activity

As the Beer-Lamberts Law is to be used in the calculation process, we shall first define it before
proceeding forward. It states that the absorbance level of a sample is proportional to the
concentration of the absorbing substance and to the path length.

Where
A

=
=
=
=

absorbance
molar absorptivity coefficient at a particular wavelength
path length
concentration of light absorbing species in the sample

Sample: S25

Time (min)
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5

A600
0.877
0.812
0.745
0.677
0.601
0.545
0.495
0.455
0.421
0.394
0.378

c (%)
0.4174
0.3867
0.3548
0.3221
0.2863
0.2597
0.2356
0.2166
0.2006
0.1878
0.1801

c (mg/ml)
4.174
3.867
3.548
3.221
2.863
2.597
2.356
2.166
2.006
1.878
1.801

600 (ml/mg)
0.21011
0.20998
0.20997
0.21018
0.20992
0.20985
0.21010
0.21006
0.20987
0.20979
0.20988

6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5

0.357
0.350
0.342
0.332
0.327
0.330
0.311
0.310

0.1702
0.1665
0.1629
0.1580
0.1556
0.1572
0.1482
0.1475

1.702
1.665
1.629
1.580
1.556
1.572
1.482
1.475

A600
0.946
0.858
0.803
0.722
1.492
0.642
0.604
0.701
0.486
0.469
0.435
0.419
0.423
0.397
0.388

c (%)
0.4503
0.4083
0.3826
0.3440
0.7103
0.3059
0.2874
0.3340
0.2316
0.2231
0.2071
0.1993
0.2012
0.1891
0.1848

c (mg/ml)
4.503
4.083
3.826
3.440
7.103
3.059
2.874
3.340
2.316
2.231
2.071
1.993
2.012
1.891
1.848

0.20975
0.21021
0.20994
0.21012
0.21015
0.20992
0.20985
0.21016

Sample: S60

Time (min)
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5

600 (ml/mg)
0.21008
0.21014
0.2099
0.20988
0.21005
0.20987
0.21016
0.20988
0.20984
0.21022
0.21004
0.21023
0.21024
0.20994
0.20996

CIII. Specific Binding Constants

% Starch

Vol. starch
(ml)

Vol. distilled
water (ml)

Total vol.
(ml)

(mg/ml)

A600

c (%)

3
2
1.5
1
0.4

3
2
1.5
1
0.4

0
1
1.5
2
2.6

3
3
3
3
3

2.658
2.533
2.197
1.471
0.394

1.2656
1.2061
1.0462
0.7003
0.1878