Control

5g
10g
15g
20g

Nitrates
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Day 8
53.942
47.882
38.742
32.842
24.742
17.674
10.942
10.942
57.325
42.048
35.049
28.994
20.843
12.045
7.449
7.449
54.464
46.943
41.994
35.053
26.853
23.945
15.965
15.965
55.949
31.043
21.535
14.995
8.667
2.943
1.585
1.585
52.631
45.942
34.604
19.003
14.783
7.943
3.743
3.743

Control
5g
10g
15g
20g

Nitrates
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Day 8
52.968
45.432
38.586
30.474
25.854
15.943
11.954
11.954
55.432
42.853
33.038
26.865
19.742
14.428
7.485
7.485
53.472
45.842
40.942
36.048
24.583
22.942
16.758
16.758
54.753
30.058
23.542
14.657
7.668
2.643
1.674
1.674
53.853
44.753
34.953
28.742
13.532
7.485
3.584
3.584
LIPID

70
Control

5g

10g

15g

60

Nitrates (mg/l)

Control
5g
10g
15g
20g

befor growth phase After growth phase end of starvation phase

Total Lipid Neutral lipidTotal
Neutral
Total
Neutral
1.8
0.6
2.78
0.9
4.87
1.47
1.8
0.6
16.6
4.5
28.4
9.743
1.8
0.6
22.4
10.9
34.17
13.34
1.8
0.6
19.4
8.8
30.406
11.5
1.8
0.6
18.3
1.9
30.221
8.1

50

20g

40
30
20
10
0

7
8
Days

10

11

12

13

14

Nitrates (mg/l)

60
50

Control

5g

40

10g

15g

30

20g

20
10
0
1

7
8
Days

10

11

12

13

14

Day 9
Day 10
Day 11
Day 12
Day 13
Day14
Day15
1.485
0.784
0.325
0.201
0.184
0.085
0.042
3.942
1.032
0.882
0.425
0.218
0.108
0.046
7.535
3.443
1.942
0.853
0.424
0.158
0.093
0.321
0.094
0.054
0.0325
0.013
0.008
0.005
1.785
1.085
0.083
0.042
0.021
0.014
0.008

Day 9
Day 10
Day 11
Day 12
Day 13
Day14
Day15
2.842
0.685
0.378
0.198
0.185
0.082
0.036
3.274
1.085
0.752
0.385
0.217
0.114
0.05
6.853
2.853
1.853
0.832
0.413
0.143
0.09
0.328
0.094
0.058
0.0328
0.016
0.001
0.006
1.542
1.048
0.084
0.048
0.017
0.015
0.009

Control
5g
10g
15g
20g

Phosphates
Day 1
40.842
42.884
44.774
42.632
40.748

Control
5g
10g
15g
20g

Phosphates
Day 1
41.836
42.773
43.596
42.374
40.586

Total Lipid
Control
5g
Befor growth phase1.8
After growth phase
2.78
End of starvation phase
4.87

10g
1.8
16.6
28.4

15g
1.8
22.4
34.17

20g
1.8
19.4
30.406

1.8
18.3
30.221

Neutral Lipid

Phosphates (mg/l)

Control
5g
Befor growth phase0.6
After growth phase0.9
End of starvation phase
1.47

0.6
4.5
9.743

15g

20g

0.6
10.9
13.34

50
45
40
35
30
25
20
15
10
5
0

0.6
8.8
11.5

0.6
1.9
8.1

Control

5g

10g

15g

20g

14

10g

7
8
Days

10

11

12

13

14

Phosphates (mg/l)
13

14

50
45
40
35
30
25
20
15
10
5
0

Control

5g

10g

15g

20g

7
8
Days

10

11

12

13

14

Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Day 8
Day 9
Day 10
35.042
28.784
22.945
14.894
12.849
5.748
5.748
2.272
0.893
36.288
28.294
20.932
14.392
7.842
3.704
3.704
0.758
0.313
35.839
27.603
17.845
12.49
8.424
3.473
3.473
1.073
0.732
38.893
33.849
26.893
18.484
13.743
7.434
7.434
3.894
0.858
34.858
27.784
19.747
11.847
8.853
3.683
3.863
0.742
0.421

Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Day 8
Day 9
Day 10
36.075
27.685
22.723
14.742
12.328
5.853
5.853
2.385
0.859
34.873
28.546
20.748
15.654
7.439
3.549
3.549
0.746
0.308
35.574
25.974
16.694
12.742
7.974
3.853
3.853
1.064
0.785
38.659
32.648
26.669
17.447
13.338
7.586
7.586
3.686
0.836
33.967
27.594
20.852
15.586
12.684
3.475
3.475
0.738
0.427

40
35

Befor growth phase

After growth phase

End of starvation phase

Total Lipid (%)

30
25
20
15
10
5
0
Control

5g

10g

15g

20g

Conditions

16

Neutral Lipid (%)

14

Befor growth phase

After growth phase

End of starvation phase

12
10
8

6
4
2
0

Control

5g

10g

15g

20g

0
Control

5g

10g
Conditions

15g

20g

Day 11
Day 12
Day 13
Day14
Day15
0.634
0.424
0.084
0.024
0.012
0.183
0.093
0.046
0.028
0.013
0.398
0.114
0.084
0.057
0.032
0.385
0.117
0.073
0.064
0.047
0.148
0.103
0.084
0.035
0.021

Day 11
Day 12
Day 13
Day14
Day15
0.639
0.428
0.089
0.027
0.011
0.185
0.096
0.048
0.024
0.014
0.385
0.118
0.082
0.051
0.038
0.347
0.116
0.064
0.042
0.032
0.137
0.105
0.084
0.031
0.026

Ph
Day 1
Control
5g
10g
15g
20g

Day 2
8.3
7.28
7.37
7.31
7.27

Ph
Day 1
Control
5g
10g
15g
20g

8.6
7.12
7.21
7.18
7.14

Day 2
7.35
7.28
7.37
7.31
7.27

Control

Day 3
8.43
6.94
7.14
7.04
6.93

Day 3
6.82
7.12
7.21
7.18
7.04

5g

10g

6.53
6.94
7.14
7.04
6.95

15g

9
8.5

Ph

8
7.5
7
6.5
6
1

7Days8

10

Day 4

Day 5
8.38
6.65
6.92
6.83
6.85

Day 4

Day 6
8.52
7.04
6.74
6.42
6.46

Day 5
6.89
6.65
6.82
6.83
6.86

Day 7
8.37
6.74
6.52
6.57
6.67

Day 6
6.5
7.04
6.74
6.42
6.63

20g

10 11 12 13 14

Day 8
8.48
6.48
6.42
6.85
6.85

Day 7
6.85
6.64
6.58
6.57
6.73

Day 9
8.53
7.32
6.73
7.23
7.32

Day 8
6.92
6.48
6.42
6.85
6.85

8.62
8.2
7.47
7.64
7.53

Day 10
Day 11
Day 12
8.51
8.73
8.69
8.38
8.42
8.64
8.17
8.38
8.46
7.87
8.35
8.54
7.75
8.21
8.36

7.25
6.62
6.63
6.86
6.58

Day 10
Day 11
Day 12
7.04
6.68
6.53
6.95
7.03
7.14
6.52
6.48
6.63
7.13
6.95
6.79
6.63
6.85
6.87

Day 9
7.08
6.75
6.74
6.64
6.74

Control
5g
10g
15g
20g

Chlora
Day 1
Day 2
Day 3
Day 4
Day 5
3.843
3.532
3.354
3.858
2.743
2.753
2.532
2.582
2.592
2.214
5.949
5.653
5.474
5.321
5.284
6.482
6.385
6.175
5.369
5.045
4.402
3.843
3.349
3.62
3.349

Day 13
Day14
Day15
6.44
6.57
6.88 Control
7.06
7.2
7.08 5g
6.45
6.72
6.83 10g
6.65
6.83
6.9 15g
6.92
6.74
6.78 20g

Day 1
Day 2
Day 3
Day 4
Day 5
3.883
3.548
3.357
3.879
2.741
2.763
2.538
2.585
2.589
2.223
5.95
5.659
5.453
5.325
5.287
6.487
6.754
6.182
5.374
5.047
4.407
3.847
3.35
3.67
3.354

Chlorophyll a(g/ml)

7
Control

5g

5
4
3
2
1
0
1

Days

8
Chlorophyll a(g/ml)

Day 13
Day14
Day15
8.78
8.72
8.64
8.81
8.73
8.75
8.58
8.74
8.65
8.32
8.21
8.37
8.26
8.38
8.43

7
6
5
4
3
2
1
0

Control

5g

10g

0
1

7 8
Days

9 10 11

Day 6
Day 7
Day 8
Day 9
Day 10
Day 11
Day 12
Day 13
Day14
2.841
2.859
2.546
2.873
2.643
2.993
2.329
2.254
2.231
2.115
2.226
2.165
2.043
2.021
2.321
2.145
2.276
2.224
5.154
5.095
4.843
4.563
4.135
3.743
3.432
3.215
3.114
4.454
4.275
4.258
4.142
3.553
3.721
3.732
2.325
2.795
3.316
3.273
2.943
2.43
2.221
2.173
2.17
2.163
2.121

Day 6
Day 7
Day 8
Day 9
Day 10
Day 11
Day 12
Day 13
Day14
2.854
2.864
2.532
2.879
2.656
2.954
2.335
2.258
2.238
2.119
2.239
2.169
2.049
2.029
2.328
2.153
2.28
2.228
5.163
5.102
4.856
4.569
4.139
3.753
3.438
3.221
3.116
4.464
4.278
4.263
4.147
3.558
3.728
3.739
2.322
2.799
3.319
3.275
2.948
2.45
2.229
2.183
2.17
2.168
2.132

10g

10g

15g

10

11

15g

20g

12

20g

13

14

10 11 12 13 14

Control
5g
10g
15g
20g

Chlorb
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
3.814
3.516
3.332
3.816
2.714
2.827
2.836
2.741
2.518
2.565
2.546
2.21
2.096
2.211
5.928
5.641
5.451
5.31
5.272
5.131
5.01
6.421
6.351
6.154
5.338
5.027
4.427
5.064
4.386
3.817
3.331
3.564
3.317
3.297
3.251
Control

5g

10g

15g

20g

7
Chlorophyll b(g/ml)

Day15
2.125
2.146
3.012
2.271
1.747

Control
5g
10g
15g
20g

6
5
4
3
2
1
0
1

7
Chlorophyll b(g/ml)

Day15
2.119
2.143
3.003
2.276
1.743

Chlorb
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
3.819
3.521
3.345
3.821
2.721
2.832
2.845
2.743
2.526
2.576
2.585
2.21
2.103
2.215
5.934
5.643
5.456
5.313
5.276
5.134
5.06
6.453
6.356
6.164
5.343
5.032
4.432
5.075
4.394
3.823
3.336
3.578
3.324
3.308
3.258

Control

7 8
Days
5g

9 10 11 12 13 14

10g

15g

6
5
4
3
2
1
0
1

7 8
Days

9 10 11 12 13 14

20g

Days

Day 8
Day 9
Day 10
Day 11
Day 12
Day 13
Day14
Day15
2.538
2.87
2.64
2.983
2.321
2.245
2.212
2.104
2.148
2.012
2.008
2.314
2.123
2.256
2.213
2.132
4.464
4.556
4.123
3.734
3.421
3.184
3.104
3.004
4.832
4.553
3.534
3.721
3.732
2.314
2.753
2.256
2.931
2.378
2.216
2.153
2.165
2.145
2.116
1.732

Day 8
Day 9
Day 10
Day 11
Day 12
Day 13
Day14
Day15
2.525
2.845
2.542
2.967
2.314
2.225
2.219
2.101
2.142
2.006
2.008
2.308
2.117
2.251
2.209
2.126
4.462
4.548
4.117
3.737
3.416
3.173
3.097
2.986
4.828
4.543
3.53
3.716
3.726
2.307
2.747
2.242
2.928
2.365
2.213
2.147
2.154
2.138
2.106
1.726

Cell density
Days
Control (12/12)
0

2
4

6
8
10
12
14

5g

10 g

15 g

1730150.5
2193882.2
2491838.8

1730150.5
2472839.2
2734349.3

1730150.5
2634492.4
2934382.3

1730150.5
1982302.3
2182394.9

2739393.3

3013824.9

3232043.4

2703843.5

2983992.2
2938381.0
2909128.6
2803289.3

3313293.3
3224090.3
3103923.5
3084239.5

3800734.9
3791991.2
3711913.5
3700482.3

2924830.1
2938430.4
2901939.3
2899372.4

Cell density
Days
Control (12/12)
2
4

6
8
10
12
14

5g

10 g

15 g

1642180.5
2152482.2
2231528.8

1580520.8
2288239.2
2684449.3

1624030.1
2434152.3
2727381.2

1560566.1
1967311.5
2292144.3

2529416.9

3093827.3

3141083.3

2407122.5

2627992.6
2878381.7
2749578.4
2608219.0

3167476.3
3034240.3
2943304.9
3035279.5

3605729.2
3584891.7
3606424.7
3627452.1

2714157.1
2627113.4
2728452.1
2698471.4

5g

15 g

Control (12/12)

10 g

20 g

4.00E+06

Cell density (cells/ml)

3.50E+06
3.00E+06
2.50E+06
2.00E+06
1.50E+06
1.00E+06
5.00E+05
0.00E+00
0

Days

10

12

14

Control (12/12)

Cell Density (cells/ml)

4.00E+06

5g

10 g

15 g

3.50E+06
3.00E+06
2.50E+06
2.00E+06
1.50E+06
0

8
Days

10

12

Initial COD

Final COD COD r %

20 g
1730150.5
2398599.2
2620409.3
2834020.5
3000492.1
2930349.4
2900340.5
2892494.4

Control
5g
10 g
15 g
20 g

300
5012
10078.8
15030.2
20040.2

38.3
900.3
1643.4
3194.3
5120.2

87.23333
82.03711
83.69449
78.74746
74.45035

CODr

20 g
1634470.6
2265261.2
2517293.3
2807432.2
2520372.1
2616240.6
2532274.1

CODr
85

CODr (%)

2625219.5

90

80
75
70
65
Control

20 g

14

5g

10 g

Days

15 g

15 g

20 g

12

14

Initial
Control (12/12)
5g
10 g
15 g
20 g

CODr

15 g

20 g

After growth Phase

End of stravation Phase
38.3
38.3
38.3
38.3
38.3

320.3
374.4
396.3
365.3
360.4

122.3
132.7
102.5
112.6
121.6

Biomass

Control
0.45
0.4
0.35

Biomass (g/l)

Control
5g
10 g
15 g
20 g

Day 1
Day 2
Day 3
Day 4
0.284
0.3
0.327
0.342
0.286
0.299
0.317
0.331
0.304
0.329
0.338
0.398
0.273
0.317
0.321
0.338
0.243
0.252
0.263
0.272

0.3
0.25
0.2
0.15
0.1
0.05
0
1

Day 5
Day 6
Day 7
Day 8
Day 9
Day 10
Day 11
Day 12
Day 13
Day 14
0.354
0.363
0.37
0.354
0.362
0.353
0.341
0.327
0.316
0.304
0.342
0.357
0.383
0.363
0.347
0.332
0.328
0.322
0.317
0.283
0.362
0.374
0.407
0.403
0.388
0.373
0.343
0.362
0.352
0.338
0.351
0.373
0.394
0.364
0.39
0.36
0.332
0.326
0.321
0.313
0.281
0.3
0.323
0.309
0.302
0.332
0.223
0.238
0.227
0.221

5g

Days

10 g

15 g

9 10 11 12 13 14 15

20 g

Biomass
Control
5g
10 g
15 g
20 g

Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
0.273
0.299
0.302
0.314
0.322
0.328
0.332
0.282
0.295
0.312
0.33
0.348
0.352
0.354
0.307
0.318
0.324
0.331
0.356
0.363
0.361
0.275
0.285
0.29
0.298
0.304
0.313
0.32
0.262
0.268
0.274
0.278
0.285
0.295
0.305

Control

5g

10 g

15 g

0.37
0.35

Biomass (g/l)

Day 15

0.33
0.31
0.29
0.27
0.25
1

Days

10 11 12

Day 8
Day 9
Day 10
Day 11
Day 12
Day 13
Day 14
Day 15
0.327
0.321
0.316
0.306
0.312
0.311
0.307
0.348
0.353
0.347
0.338
0.332
0.324
0.318
0.363
0.356
0.351
0.346
0.341
0.338
0.33
0.311
0.298
0.287
0.281
0.276
0.262
0.257
0.3
0.294
0.287
0.282
0.273
0.267
0.261

15 g

12 13 14

20 g