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State that enzymes are globular proteins, with a specific tertiary structure, which catalyse metabolic reactions in living organisms
Specificity, Active Site, Enzyme-substrate complex and Enzyme-product complex All enzymes contain an active site which is usually a depression within the enzyme that another molecule can bind to. This molecule is a substrate, and the substrate must be the exact same shape as the enzyme in order for it to bind and create an enzymesubstrate complex. The substrate must be specific to the active site. Once the enzyme-substrate complex is made, it catalyses the reaction. This may cause the substrate to split into two or more molecules, or catalyse the Describe, with the aid of diagrams, the joining together of two molecules. Either way, it forms mechanism of action of enzyme molecules, an enzyme-product complex and from that two or with reference to specificity, active site, more products. lock and key hypothesis, induced-fit hypothesis, enzyme-substrate complex, enzyme-product complex and lowering of activation energy
Lock and Key Hypothesis The lock and key hypothesis basically states that the substrate fits the active site perfectly without any help or change in shape. This is shown in the image above.
(cont.) Describe, with the aid of diagrams, the mechanism of action of enzyme molecules, with reference to specificity, active site, lock and key hypothesis, induced-fit hypothesis, enzyme-substrate complex, enzyme-product complex and lowering of activation energy
1. Set up the equipment needed to carry out the test. 2. In one test tube, add a measured amount of substrate and a measured amount of enzyme. Add either an acid or alkali and take tests at varying pH levels. Describe how the effects of pH, temperature, enzyme concentration and substrate concentration on enzyme activity can be investigated experimentally 3. In another, do the same again but put a thermometer in the tube and heat it up, measuring the rate of reaction every xo. 4. In another, add a set amount of substrate but add a lot of enzyme solution and take tests. 5.In another add a set amount of enzyme solution but add a lot of substrate solution and take tests.
State that some medicinal drugs work by inhibiting the activity of enzymes