Technical File Exossine: Exo-P

INTRODUCTION
Industrialization and progress come with a price. Human activities release chemicals, gases, particles, and dust into the atmosphere. Pollutants are heavily concentrated in the cities with important consequences for the human body. Urban pollution causes acidification of the skin and disturbance of hydration with a concomitant increase in transepidermal water loss. Poor hydration is associated with visible changes at the skin surface and loss of protection from external aggression. City people often complain of a dull complexion. This is related to the fact that pollution affects skin texture through increase skin flaking and reduced skin flexibility. On top of that, there is strong evidence suggesting a crucial role for environmental factors in the aggravation of pathological skin conditions such as xerosis, atopic dermatitis, acne, and psoriasis. Protection from urban pollution is thus a prime target for skin appearance and skin health. But how can we Support the Natural Protective Barrier Function of the Skin? The Answer may come from Nature Itself In this work, we describe the in vitro, ex vivo, and clinical data supporting the use, for cosmetic applications, of the third exotic EPS originating form the Kopara surrounding Moorea atoll in French Polynesia. Following the launch of Exo-H and Exo-T, we now introduce: Exo-P Exo-P chelates heavy metals and protects against toxic chemicals and thus providing complete protection against aggressions resulting from urban pollution. Exo-P is the key ingredient to improve rapidly dull skin for a healthy and luminous skin complexion.

EFFICACY STUDIES
Detoxification from Heavy Metals with Exo-P
BACKGROUND Exopolysaccharides have the remarkable ability to make chemical bonds with heavy metal ions, thus rendering them much less chemically reactive. In line with these known properties of exopolysaccharides, the chelation potential of Exo-P toward cadmium and lead was measured to document its potential as a protective agent. CONCLUSION The ability of Exo-P to adsorb divalent cations may facilitate heavy metal removal from the surface of the skin thus offering a protection from common urban pollutants such as cadmium and lead.

Prevention Effect by Exo-P from UV-Induced Lipid Peroxidation

BACKGROUND UV exposure is a potent oxidant challenge for skin cells. UV-B radiation generates the production of reactive oxygen species (ROS) among which hydrogen peroxide (H2O2) known to induce damage to lipids, proteins, and DNA (Briganti and Picardo, 2003). Peroxidation of lipids in cell membranes alters their fluidity and their signaling efficiency, leading to inflammatory changes, premature aging of the skin, and aberrant cell proliferation (Bickers and Athar, 2006).
1

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Technical File Exossine: Exo-P

RESULTS Results obtained in fluorescence have been transformed to express protection of keratinocyte membrane from radical formation. Not surprisingly, UV exposure of cells results in a significant increase in the level of lipid peroxidation at the cell membrane. In the same conditions, the synthetic anti-oxidant BHA (positive control) reduced the formation of lipid peroxidation by 76%, while the protection conferred by ExoP was of 28%. CONCLUSION

Effect of Exo-P on lipid peroxidation in keratinocytes

% peroxidation
100 80 60 40 20 0 Control Control + UVB BHA Exo-P 0,04% + UVB Exo-P 0,1% + UVB
* p<0.05

24% protection

28% * protection

76% *

Exo-P significantly protects skin cell membranes from lipid peroxidation under UV exposure. Protection from UV-induced lipid peroxidation is associated with prevention of skin photo-aging.

Protection from Urban Pollution by Exo-P Ex Vivo Lipid Peroxidation Study

BACKGROUND In addition to heavy metals and UV radiation, there are other environmental hazards susceptible of affecting the integrity of human skin. These include cigarette smoke, fuel exhaust, halogenated hydrocarbons, and ozone. In all cases, free radicals are generated upon exposure leading to increase oxidative reactions including peroxidation of lipids at the membrane of cells (Pelle et al, 2002; Scherer, 2005). Malondialdehyde (MDA), the end product of lipid peroxidation has been used here as a biomarker of oxidation in an ex vivo model of skin exposure to urban pollution represented by a pollution cocktail, a mixture of heavy metals and hydrocarbons. These pollutants are representative of what can be found in cigarette smoke and fuel exhaust. The model allowed us to evaluate the protective potential of Exo-P. CONCLUSION Exo-P protects skin explants from lipid peroxidation under urban pollutant exposure. Preserving skin lipids from oxidation is known to be important for the maintenance of the barrier function of the stratum corneum.

Protection from urban pollution by Exo-P in skin explant

700

MDA pmol/ml

650 600 550 500

44% protection
76%

Basal

Pollutant

Pollutant + tocopherol

Pollutant + Exo-P 3%

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Technical File Exossine: Exo-P

Anti-Pollution Consumer Test

OBJECTIVE The objective of this study was to evaluate the consumer perception of Exo-P as a fast action anti-pollution active following 7 days of treatment. PROTOCOL Subject 57 woman volunteers aged 30 to 50, living in a big city, and with a skin sensitive to pollution. Half of the volunteers were smoker (29 out of 57). Test conditions Each volunteer applied a cream containing Exo-P 2% on their face, twice a day (morning & night) for 7 days. Subjects were asked to fill out a self-evaluation questionnaire at the end of the study (D7), in order to evaluate their overall opinion and feeling on the effectiveness of the product. RESULTS

Immediate well-being Improvement in dullness Resistance to external aggressions Shielding effect Protection from pollution Healthy looking skin Luminous skin tone Prevention of skin irritation & redness 70% Positive Opinion (%) 74% 77% 77% 79%

82% 82% 82%

CONCLUSION
Exo-P provides an effective shield against aggressions resulting from urban pollution. The skin looks prettier and healthier with a luminous tone. Exo-P is the perfect defense for SKIN IN THE CITY. This sophisticated natural active is well-defined exotic polysaccharide obtained from Kopara living in unique ecosystems in the French Polynesia. Naturally rich in essential minerals, they shield the beauty of skin. Exo-P provides a complete protection against aggression resulting form urban pollution.
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Technical File Exossine: Exo-P

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Technical File Exossine: Exo-P

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Technical File Exossine: Exo-P

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