UNIVERSITY OF SANTO TOMAS GRADUATE SCHOOL

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A Thesis Proposal Presented to the Grad ate !"hool Uni#ersit$ o% !anto To&as

In Partial F l%ill&ent O% the Re' ire&ents o% the De(ree Master o% !"ien"e in )iolo($

*$ +UAN CARLO! DE )OUR)ON No#e&*er ,-.-

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TA&LE O' CONTENTS Pa(e Pre%ator$ Note 0Optional1 1(0 T)e Pro*lem Ratio!ale 3 Introd "tion 3 Resear"h I&pedi&ents 2(0 T)e Re"ear+) ,-e"tio!" 2(1 Literat re Re#ie/ 6 2(2 Resear"h 7 estions ,, .(0( T)e Re"ear+) /et)o%" .(1 Methods and Materials ,8 9.... !tandards2 Rea(ents and Che&i"als ,5 9..., E:tra"tion 0or !a&plin(1 Pro"ed re ,6 9...9 Ethi"al Considerations 0123 Appli+a*le45 9, .(2 Anal$sis 9.,.. Instr &ental Anal$sis ,; 9.,., Data Interpretation and Cal" lations ,< Re3ere!+e" 99 Examples only! (remove those not applicable) Appendi: I4 Per&it to Cond "t the Inter#ie/s 96 Appendi: II4Re' est %or Pertinent Data 9; .

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Appendi: III4 In%or&ed Consent %or Resear"h Parti"ipants 9< Appendi: I=4 In%or&ed Consent %or Resear"h Parti"ipation Re"r ited Con"ept Mappin( Parti"ipants 3Appendi: =4 ! r#e$ 7 estionnaires 3. Appendi: =I4 The Inter#ie/ Proto"ol 33 Appendi: =II4 The Inter#ie/ Proto"ol %or T tors>Re#ie/ers 35 Appendi: =III4 The Inter#ie/ Proto"ol 36 Appendi: I?4 Con"ept Mappin( Instr &ent 3; Appendi: ?4 Do" &entar$ Anal$sis 3< Appendi: ?I4 Ti&eta*le %or Resear"h 8Appendi: ?II4 ) d(et Proposal 8. C-rri+-l-m 6itae 8,

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CHAPTER . THE PRO)LEM RATIONALE

This "hapter & st *e "lear and lo(i"al in des"ri*in( succinctly the trends in the %ield that render the "hosen topi" problematic. This "hapter & st end /ith the delineation o% the re"ear+) *arrier" or limitatio!" o% the st d$2 0if possible, s *stantial2 ethi"al2

&ethodolo(i"al2 and pra"ti"al1 that the "andidate &a$ "o&e a"ross in the "ond "t o% the st d$. The last t/o para(raphs & st *e de#oted to resear"h i&pedi&ents 0*arriers1 and li&itations o% the st d$. The %irst para(raph o% the paper *e(ins /ith the %irst line o% the para(raph indented one@hal% in"h 08@6 spa"es1. There are no additional lines added *et/een para(raphsAB st the re( lar do *le spa"e that sho ld *e &aintained thro (ho t the paper and the Re%eren"es pa(e2 i% there is one. Do not se *lo"C para(raphin( 0e:tra lines *et/een para(raphs2 /ith ne/ para(raphs *e(innin( %l sh le%t /itho t indentation1 %or a"ade&i" papersD s "h %or&at is in"reasin(l$ sed these da$s %or * siness letters2 * t not %or a"ade&i" or s"holarl$ papers.

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The PAGE NUM)ER o% the %irst pa(e o% EACH CHAPTER & st not appear 0and in this te&plate2 /as *lo"Ced /ith an opa' e s' are1. The s ""eedin( pa(es MU!T sho/ proper pa(e n &*ers.

UNIVERSITY OF SANTO TOMAS GRADUATE SCHOOL

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CHAPTER , THE RE!EARCH 7UE!TION!

This "hapter in"l des all t$pes o% &aterials re#ie/ed2 "on"ept al literat re /hi"h "a&e %ro& *ooCs. Related st dies *oth lo"al and %orei(n "onsistin( &ainl$ o% peer re#ie/ed Bo rnals. Yo r ai& is to sho/ )o$ the present st d$ relates to the exi"ti!7 #!o$le%7e and pre8io-" st dies in ter&s o% *oth si&ilarities and di%%eren"es. The %or&al s *@se"tions %or this "hapter areD Be more judicious in making Section headings that are nne"essar$ and inappropriate. I% it ne"essitates headin(s2 or $o are /ritin( a lon( paper in /hi"h headin(s are needed *$ "on#ention or to help $o r reader2 %ollo/ APA %or&at re' ire&ents as e:plained in the A&eri"an Ps$"holo(i"al Asso"iation EAPAF 0,--.1 Publication Manual. Conser#ati#e s"holarl$ standards in &ost dis"iplines re' ire stri"t third-person sa(e in reports o% e:peri&ents and st dies. APA r les allo/ %or the se o% the first person /hen appropriate2 to a#oid the a/C/ardness o% e:"l si#e third@person sa(e. A detailed dis" ssion o% this iss e2 &a$*e %o nd on se"tion 9.-< 0Pre"ision and Clarit$2 APA 0,-.-1 Publication Manual2 p.5;2 6th edition1. The

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s *se"tion on GAttri* tion2H list e:a&ples o% ndesira*le attri* tion in third-person sa(e /hen des"ri*in( steps taCen in the Methodolo($ se"tion. 2(1 Re8ie$ o3 t)e Literat-re M st *e +ompre)e!"i8e in the li(ht o% the "hosen #aria*les /ith a #ie/ to s r%a"in( the resear"h blankspots 0 ne:plored area1 and blindspots 0"on%li"tin( areas in the literat re1. 2(2 Re"ear+) ,-e"tio!9" 0or H pot)e"e"1 A resear"h h$pothesis is the state&ent $o "reated /hen $o spe" late pon the o t"o&e o% a resear"h or e:peri&ent. E#er$ tr e e:peri&ental desi(n & st ha#e this state&ent at the "ore o% its str "t re2 as the lti&ate ai& o% an$ e:peri&ent.

UNIVERSITY OF SANTO TOMAS GRADUATE SCHOOL

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CHAPTER 9 THE RE!EARCH METHOD!

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%ollo/in(D 0.1 resear"h desi(n2 0,1 st d$ site2 091 sele"tion o% sa&ples2 031 data>o t"o&e &eas res 2 data "olle"tion pro"ed re2 081 et)i+al +o!"i%eratio! and 051 data>&ode o% anal$sisD and M st *e "tro!7l s pported *$ the appendices that "ontain the instr &ents

or "orp s o% data to *e sed. There are t$o 3ormal s *@se"tions 09.. and 9.,1 /hi"h areD .(1 /et)o%" a!% /aterial" .(1(1 !tandards2 Rea(ents2 and Che&i"als This se"tion & st ha#e detailed des"ription o% &aterials sed. .(1(2 E:tra"tion 0or !a&plin( or !$nthesis1 Pro"ed re This se"tion +a! )a8e eit)er o% the 9 titles dependin( on $o r resear"h. .(2 A!al "i" .(2(1 Instr &ental Anal$sis This se"tion & st ha#e %etaile% des"ription o% &aterials sed. .(2(2 Data Interpretation and Cal" lations

UNIVERSITY OF SANTO TOMAS GRADUATE SCHOOL In this se"tion $o

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&a$ in"l de $o r statisti"al anal$sis2

des"ription o% so%t/ares et". 'or example 0NOTE: t)i" $a" ta#e! 3rom a ;o-r!al( "i!+e o- are $riti!7 a propo"al< t)e 7rammar GM! and M!>M! data

3orm /UST &E 2N THE 'UTURE TENSE5 sear"hes /ere per%or&ed

sin( a lo"al Mas"ot ser#er 0Mas"ot ,.,.-2

Matri: !"ien"e2 London2 UI1 a(ainst the !/issJProt data*ase 0#ersion 85.;2 3.-2 8.; se' en"es1. For MALDIJM! peptide &ass

%in(erprintin(2 a &ass toleran"e o% 8- pp& /as allo/edH. 0ta#e! 3rom 'o-ille! et al(< Analytical Biochemistry =0> 020105 .=? =.5

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)I)LIOGRAPHY In"l de all literat re a+t-all "ited in "hapters . to 92 arra!7e se APA

e!trie" ALPHA&ET2CALL@ 0NOT NU/&ERED5D stri"tl$

"t le e!trie". !a&ple entries %or *oo#"2 ;o-r!al"2 *oo# e%itio! and i!ter!et "o-r+e are sho/n *elo/ in that orderD )a:ter2 C. 0.<<61. Ra"e E' alit$ in Health Care and Ed "ation. Philadelphia4 )alliKre Tindall. Ga dio2 +. L.2 L !no/don2 C. T. 0,--;1. !patial C es More !alient Than Color C es in Cotton@Top Ta&arins 0 aguinus oedipus1 re#ersal learnin(. !ournal of "omparati#e Psychology 2 .,,2 33.@333.

doi4.-..-96>-698@6-95..,,.3.33. H$de2 +. !.2L Dela&ater2 +. 0,--;1. H &an !e: alit$ 0.-th ed.1 Ne/ YorC4 M"Gra/@Hill. Resear"h Initiati#es. 0n.d.1. Retrie#ed +an ar$ ..2 ,--62 %ro& MIT2 Co&parati#e Media !t dies /e*site2

http4>>"&s.&it.ed >resear"h>inde:.php

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Appe!%ix 2

Timeta*le 3or Re"ear+)

Re"ear+) O*;e+ti8e" .. Mritin( o% Proposal ,. Colle"tion o% Al(al Materials 9. Fra"tionation o% Pol$sa""harides 3. Colle"tion o% )lood and Isolation o% P)MCs 8. Cell =ia*ilit$ Assa$ 5. ELI!A Assa$ %or C$toCines 6. MRNA e:tra"tion ;. Che&opre#enti#e assa$s <. Data Collation>Anal$sis .-. Collo' i &>De%ense

2010
A! Al A7 Se pt O+ t No 8 De + Aa ! 'e * /a r

2011
Ap r /a

UNIVERSITY OF SANTO TOMAS GRADUATE SCHOOL Appe!%ix 22 &-%7etar ReB-ireme!t" ,-a!tit

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SOURCES O' EXPENSES Colle+tio! a!% 'ra+tio!atio! o3 Pol "a++)ari%e" Colle"tion o% !ar(ass & ;-N Ethanol -..M HCl ..,8M NaOH "et$l p$ridini & "hloride <<N a"eti" a"id <<N ETOH L$ophiliOation &loo% Colle+tio! a!% 2"olatio! o3 P&/C" #a" tainer t *es Fi"oll@H$pa' e (radient soln Ti""-e C-lt-re Rea7e!t" L@(l ta&ine Peni"illin@!trepto&$"in Hepes ) %%er !odi & *i"ar*onate Tr$psin -.,8N EDTA MTT DMEM F)! ' ali%ied H. !td. TC %lasC 8- &l "anted ne"C .8 &l "oni"al t *e / "ap pol$ 8- &l "oni"al t *e /> "ap pol D l*e""os Phosphate ) %%ered RPMI .53Tr$pan )l e Rea(ents FreeOin( Medi & DM!O !er & %ree !odi & P$r #ate !oln DPPH rea(ent Hep G, Cell Lines

Amo-!t 0P)P5 .-2--82658.-,,.5--9<95-,8-2C<=C5 6-.82--15>00 ,2.,5.6< .2638.83 .25--.;< ;8<.;, <66.5; 62955.-6 925;, <28...5. 325,8 32 ;68 32<5;.53 ,2 3.6 8296, .233< 523;3 <-, 62,9; 982--D.<D0D(0 =

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UNIVERSITY OF SANTO TOMAS GRADUATE SCHOOL

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RNA 2"olatio! a!% RT-PCR rea7e!t" TriOol rea(ent RNAse O t Re"o&. Inhi*itor RT@PCR Mi: Rea"tions A(arose ele"tro rea(ents M Tris ) %%er sol tion Gel Pilot DNA loadin( d$e Pipet tips $ello/ PCR t *e (lo#es Tiss es and "leanin( rea(ents EL2SA F2T Rea7e!t" IL@5 H &an anti*od$ IL@. H &an anti*od$ TNF@P H &an Anti*od$

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