Professional Documents
Culture Documents
• Introduction
• Control of Gene Expression in Prokaryotes
• The lac Operon of E. coli
• The trp Operon of E. coli
• Control of Gene Expression in Eukaryotes
o Control of Transcriptional Initiation
Introduction
The controls that act on gene expression (i.e., the ability of
a gene to produce a biologically active protein) are much
more complex in eukaryotes than in prokaryotes. A major
difference is the presence in eukaryotes of a nuclear
membrane, which prevents the simultaneous transcription
and translation that occurs in prokaryotes. Whereas, in
prokaryotes, control of transcriptional initiation is the major
point of regulation, in eukaryotes the regulation of gene
expression is controlled nearly equivalently from many
different points.
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Attenuation of the trp operon. The attenuation region of the trp operon
contains sequences that allow the resulting mRNA to form several different
stem-loop structures. These regions are identified as 1 through 4. The stem-
loops that are significant as to whether transcription is attenuated or not are
formed between regions 2 and 3 or between regions 3 and 4. When tryptophan
levels are high there is plenty of charged trp-tRNAs available and ribosomes
translating the leader peptide encoded by the trpL gene do not stall at the
repeated trp codons in the leader peptide. Under these conditions the
ribosomes rapidly cover regions 1 and 2 of the mRNA which allows the stem-
loop composed of regions 3 and 4 to form. The stem-loop formed by regions
3-4 results in a transcriptional termination structure and transcription of the trp
operon ceases, i.e. is attenuated. Conversely, when tryptophan levels are low
the level of charged trp-tRNAs will also be low. This leads to a stalling of the
ribosomes within the leader peptide when they encounter the trp codon
repeats. The ribosome stalls over region 1 of the mRNA which allows step-
loop 2-3 to form and prevents the transcriptional termination stem-loop 3-4
from forming. The inability of this structure to form allows the entire operon
to be transcribed and the tryptophan biosynthetic enzymes to be produced.