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Abstract
Alginate production from glucose-based media by Azotobacter vinelandii DSM 576 was studied in the shaken-flask scale at different temperatures ( !"# $%&' shaking speeds ( 5("#5( min )*&' and initial concentrations of yields glucose (*("*(( g l)*&+ ,hen buffering the culture medium' alginate ( & and bacterial biomass were optimi-ed with respect to the ratio and acetate (Ac& and phosphate (.h& concentrations by performing a composite design e/periment+ 0he e/perimental yields were fitted to the only statistically significant factors with mean percentage errors smaller than *51 and * 1' respecti2ely+ 0he resulting empiric models were used to estimate the ratio (**( g-atom % per gatom 3& and concentrations of acetate and phosphate associated with optimum alginate yields ranging from 5 to !!1 of the glucose supplied+ More specifically' phosphate limitation ((+(!5 g l )*& and no acetate addition resulted in ma/imum alginate production with respect to the case of e/cess of phosphate (!+5 g l)*& and acetate (! g l)*&+ 4urther trials permitted us to assess that the alginate yield was hampered by any sodium acetate addition5 howe2er' 6A78 was insensiti2e to .h concentrations and ratios in the ranges (+(!5"*+9 g l)* and 6("**( g-atom % per g-atom 3' respecti2ely' in acetatefree media+
Keywords
Alginate5 bacteria5 Azotobacter vinelandii5 polysaccharide5 optimi-ation5 e/perimental design
Keywords
Alginate5 Azotobacter vinelandii5 ?/opolysaccharide5 Microfiltration5 Membrane bioreactor