Biotechnology SM CLASSES

Question 1 A dividing and undifferentiated mass of cells is called A B C D Callus Embryo Explant Zygote

Question 2 Advancement in genetic engineering has been possible due to the discovery of A B C D Oncogenes Transposons Restriction endonuclease Exonucleases

Question 3 Agarose extracted from sea weeds finds use in A B C D Gel electrophoresis Spectrophotometry Tissue culture PCR

Question 4 Continuous addition of sugars in 'fed batch' fermentation is done to A B Degrade sewage Produce methane

C D

Obtain antibiotics Purify enzymes

Question 5 Which purine is found in RNA? A B C D Guanine Cytosine Thymine Uracil

Question 6 DNA fingerprinting was discovered by A B C D James Watson Alec Jeffreys Frederick Fanger Hargobind Khorana

Question 7 DNA is found in A B C D Chromosomes Plastids Mitochondria All of the above

Question 8 Dolly, The first cloned mammal, was produced by A B Parthenogenesis Artificial fertilization

C D

Replacement of zygote nucleus by somatic nucleus in vitro fertilization

Question 9 Embryo culture is used for A B C D Establishing suspension culture Recovery of inter specific hybrids Somatic hybridization Haploid production

Question 10 First artificial gene was synthesized by A B C D Nirenberg Mendel Morgan Khorana

Question 11 Genetic Engineering is A B C D plastic surgery addition or removal of genes study of extranuclear genes all of the above

Question 12 Genomics is the study of A Genes in general

B C D

Human genes Genomes in general Human genome

Question 13 Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5' ______ GAATTC ________ 3' 3' ________CTTAAG ________ 5' A B C D Palindromic sequence of base pairs Replication completed Deletion mutation Start codon at the 5' end

Question 14 Maximum number of existing transgenic animals is of A B C D Pig Fish Mice Cow

Question 15 One of the following processes involves southern blotting technique A B C D rDNA technology DNA finger printing production of transgenic plants formation of suspension culture

Question 16 The transgenic animals are those which have A B C D foreign DNA in some of their cells foreign DNA in all of their cells foreign RNA in all of their cells Both (A) and (C)

Question 17 The two strands of DNA are held together by bonds of A B C D Nitrogen Oxygen Hydrogen Carbon

Question 18 There is a restriction endonuclease called EcoRI. What does "co" part in it stand for? A B C D coli colon coelom coenzyme

Question 19 Transgenic plants are developed by A B C Introducing foreign genes Introducing gene mutations Deleting certain chromosomes parts

Stopping spindle formation

Question 20 Cry genes or Bt genes are obtained from A B C D Cotton pest Tobacco plant Bacillus thuringiensis E - Coli

Question 21 Which enzyme is useful in genetic engineering? A B C D DNAase Amylase Lipase Restriction endonuclease

Question 22 Which is related to genetic engineering? A B C D Plastid Plasmid Heterosis Mutation

Question 23 Which of the following enzyme joints the okazaki fragments? A B C DNA polymerase DNA ligase Helicase

Restriction endonuclease

Question 24 Which of the following features should be present in vector? A B It should replicate autonomously It should have its own origin of replication It should possess restriction enzyme cleavage sites All of these

C D

Question 25 Which of the following is a genetic vector? A B C D Plasmid Phage Cosmid All of these

Question 26 Which of the following is related to genetic engineering? A B C D Mitochondrion Golgi apparatus Lysosome Plasmid

Question 27 Which of the following is used in Biowar? A A pathogen

B C D

Toxin from a pathogen A delivery system for the bioweapon agent All of these

Question 28 Which of the following tool makes possible genetic engineering? A B C D Restriction endonuclease DNA polymerase Helicase RNA polymerase

Question 29 Which one contains four pyrimidine bases? A B C D GATCAATGC GCUAGACAA UAGCGGUAA TGCCTAACG

Question 30 Which one is found only in RNA? A B C D Cytosine Adenine Uracil Guanine

Question 3 1

Which one of the following is used as vector for cloning genes into higher organisms? A B C D Salmonella typhimurium Retrovirus Baculovirus Neurospora crassa

Question 32 The vector for T-DNA is A B C D Thermus aquaticus Salmonella typhimurium Agrobacterium tumefaciens Bacillus thuringiensis

Question 33 Cellular totipotency is demonstrated by A B C D All plant cells Only gymnosperm cells All eukaryotic cells Only bacterial cells

Question 34 Crop plants grown in monoculture are A B C D Characterized by poor root system Free from intraspecific competition Low in yield Highly prone to pests

Question 35

Differentiation of organs and tissues in a developing organism, is associated with A B C D Differential expression of genes Developmental mutations Deletion of genes Lethal mutations

Question 36 Meristem culture is practiced in horticulture to get A B C D Slow-growing callus Somaclonal variation Haploids Virus-free plants

Question 37 Micropropagation is a technique A B C D for production of true to type plants for production of haploid plant for production of Somatic hybrids for production of Somaclonal plants

Question 38 What is true of plasmid? A B C D Part of nuclear chromosome Contains genes for vital activities Found in viruses Widely used in gene transfer

Question 39 Somaclonal variation appears in plants A B C D Transformed by recombinant DNA technology Growing in polluted soil or water Exposed to gamma rays Raised in tissue culture

Question 40 Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells) A B C D An antifeedant A particular hormone Both sense and anti-sense RNA A toxic protein

Question 41 Plasmids are suitable vectors for gene cloning because A these can shuttle between prokaryotic and eukaryotic cells these are small circular DNA molecules with their own replication origin site these are small circular DNA molecules, which can integrate with host chromosomal DNA these often carry antibiotic resistance genes

Question 42

Some of the characteristics of Bt cotton are A B C Medium yield, long fibre and resistance to beetle pests Long fibre and resistance to aphids High yield and resistance to bollworms High yield and production of toxic protein crystals which kill dipteran pests

Question 43 Cultivation of Bt cotton has been much in the news. The prefix Bt means ______. A produced by biotechnology using restriction enzymes and ligases bigger thread variety of cotton with better tensile strength barium-treated cotton seeds carrying an endotoxin gene from Bacillus thuringiensis

B C D

Question 44 Stirred-tank bioreactors have been designed for A B Purification of the product Addition of preservatives to the product Availability of oxygen throughout the process Ensuring anaerobic conditions in the culture vessel

Question 45 Restriction endonucleases are enzymes which A Recognize a specific nucleotide sequence for binding of DNA ligase Make cuts at specific positions within the DNA molecule Remove nucleotides from the ends of the DNA molecule Restrict the action of the enzyme DNA polymerase

Question 46 Bacillus thuringiensis is used to control A B C D Nematodes Fungal pathogens Bacterial pathogens Insect pests

Question 47 The genetically-modified (GM) brinjal in India has been developed for A B C D Enhancing shelf life Insect-resistance Drought-resistance Enhancing mineral content

Question 48 The protein products of the following Bt toxin genes cry IA c and cry II Ab are responsible for controlling

A B C D

Bollworm Roundworm Moth Fruit fly

Question 49 Variable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in A B C D Monoclonal antibody production DNA fingerprinting Recombinant DNA technology Stemcell culture

Question 50 Silencing of mRNA has been used in producing transgenic plants resistant to A B C D Bollworms Bacterial blights White rusts Nematodes

Question 51 Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from A B C D Bacteriophages Bacterial cells Plasmids All prokaryotic cells

Question 52

PCR proceeds in three distinct steps governed by temperature, they are in order of A B C D Annealing, Synthesis, Denaturation Synthesis, Annealing, Denaturation Denaturation, Annealing, Synthesis Denaturation, Synthesis, Annealing

Question 53 Which of the following is correctly matched? A B C D Ligase - Molecular scissors Thermus aquaticus - Bt-gene Agrobacterium tumefaciens Tumour Hind II - Plasmid vector

Question 54 Sterilization of tissue culture medium is done by A mixing the medium with antifungal agents filtering the medium through fine sieve autoclaving of medium at 120 for 15 min keeping the medium at 20C

C D

Question 55 Biolistics (gene-gun) is suitable for A B Constructing recombinant DNA by joining with vectors Transformation of plant cells

C D

Disarming pathogen vectors DNA finger printing

Question 56 An improved variety of transgenic basmati rice A Gives high yield and is rich in vitamin A Does not require chemical fertilizers and growth hormones Give high yield but has no characteristic aroma Is completely resistant to all insect pests and diseases of paddy

Question 57 In cloning of cattle a fertilized egg is taken out of the mother's womb and in the eight cell stage the individual cells are separated under electrical field for further development in culture media the egg is divided into 4 pairs of cells which are implanted into the womb of other cows in the eight cell stage, cells are separated and cultured until small embryos are formed which are implanted into the womb of other cows. from this up to eight identical twins can be produced

Question 58

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? A B C D 5 CACGTA 3; 3 CTCAGT 5 5 CGTTCG 3; 3 ATGGTA 5 5 GATATG 3; 3 CTACTA 5 5 GAATTC 3; 3 CTTAAG 5

Question 59 Assertion: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote). Reason: Both bacteria and yeast multiply very fast to form huge population, which express the desired gene. Both Assertion and Reason are true and the Reason is the correct explanation of the Assertion. Both Assertion and Reason are true but the Reason is not the correct explanation of the Assertion. Assertion is true statement but Reason is false. Both Assertion and Reason are false statements.

Question 60 Compare the statements A and B. Statement A: Synthesis of DNA takes place in the S-phase of interphase. Statement B: Every chromosome, during metaphase, has two chromatids. Choose the correct description : A Statement A is correct and B is

wrong. B Statement A is wrong and B is correct. Both the statements A and B are correct and A is not the reason for B. Both the statements A and B are correct and A is the reason for B.