T ECHNI CAL DAT A S HEET OF DI ARS T OP APPLI CAT I ON I N PRODUCT I VE B REEDI NG FACI LI T I ES

I NT EGRAL ROAS T ED CEREAL COMPLE ME NT ARY FEED FOR ALL ANIMAL SPECIES.

E U R OF E E D T E CH N OL OGI E S s .r .l. thanks to ex tens ive ex per ience and ex per iments in lives tock far ms , have developed a natur al vegetable pr oduct able to impr ove animals yield, by a cor r ect modulation in the development of micr oor ganis m which ar e pr es ent in the diges tive appar atus thanks to the D I AR S T OP vegetable components , well dos ed among thems elves and elabor ated by innovative technological s ys tems , ar e able to impr ove the diges tive appar atus s tate of health, s omtimes in danger becaus e of the alter ation of the micr obial intes tinal flor a typical in intens ive br eeding. D I AR S T OP acts with a phys ical pr otection cr eating a film on the gas tr ointes tinal walls and in this way PREVENT S GAS T RO- ENT HERI C DI S E AS ES (necr otic enther itis , gas tr o- enther itis , as pecific diar r ohea and dehydr ation etc.). I t has a deconges ting action on mucos ae and I MPROVES T HE AB S ORB T I ON of NUT RI ENT S and ENHANCES T HE EFFE CT I VENES S OF T HE RAPI ES AGAI NS T GAS T RO- ENT HERI C DI S E AS ES . D I AR S T OP helps impr oving the health of animals that ar e s tr es s ed by diets with high pr otein content or br ed in inadequate facilities or technologies . I t r egular iz es the s peed of the tr ans it in the chimo, keeps intes tines clean, impr oves the abs or btion of nutr ients , r educes abdominal s welling and flatulences , faeces become mor e compact. I ts ar oma impr oves appetite. I t can be us ed: Fr om the br eeder s as feed additive, in par ticular for lactating animals . As s uppor t fr om integr ator is ti, as a s ubs titute of aux inic antibiotics and/or chemiopr ofilax ic for animals that have enther ic dis eas es . Added to indus tr ial feed to pr event a lar ge par t of enther ic dis eas es , impr oving gr owth and yields . After s ever al tr ials that have been car r ied out in far ms ther e has been a r eduction of diar r ohea and a better gr owth in compar is on to non tr eated animals and animals tr eated with aux inic pr oducts .
1

COMP ON E N T S I ntegr al r oas ted bar ely meal integr al r oas ted oat meal I ntegr al r oas ted r ice meal I ntegr al r oas ted wheat meal

AN AL Y S I S
Ar s enico 0,019 Cadmium 0,012 mg/K g Mer cur e < LQ mg/K g L ead 0,041 Water 1,1% T otal pr otein 9,3% T otal fats 2,2% Cr ude fibr e 11,8% As hes 4,8% S tar ch 17,5% Copper as Cu 3,9 Z inc as Z n 21,7 I r on as Fe 29,9 T otal Phos phor us as P S ulphates 430 Chlor ides 895,4 Calcium as Ca 571 Magnes ium as Mg Potas s ium as K 0,62% Fr uctos e 0,30% Maltos e 1,20% I ns etticidi Or gano Clor ur ati abs ent I ns etticidi Or gano Fos for ati abs ent I ns etticidi Car bammati Gr uppo 1 abs ent Aflatox ins Deter mination inHPL C Aflatox in B 1 abs ent Aflatox in B 2 abs ent Aflatox in G1 abs ent Aflatox in G2 abs ent mg/K g mg/K g

t.q. t.q. t.q. t.q. t.q. t.q. ppm t.q. ppm t.q. ppm t.q. 0,5% t.q. ppm t.q. ppm t.q. ppm t.q. 0,16% t.q. t.q. t.q. t.q.

D OS AGE S : W H I T E ME AT CAL VE S Fr om the 2 nd to the 30 th day 10 g/animal/meal. Fr om the 31 s t day to the s laughter ing fr om 10 to 20 g/animal/meal. I n cas e of enter itis 50- 100 g/animal/meal till the pr oblem is s olved. N .B . D I AR S T OP impr oves the quality and the appear ance of meat and the ir on it contains , s ince it by- pas s es , does not dar ken its color , but makes the meat whiter .I n or der to r each the r ight weight in advance, dur ing the las t per iod incr eas e by 8- 12% the dos age of milk and of D I AR S T OP to 20- 25 g/animal/meal. I n cas e of dys en t er y w e w ou l d r ecom m en d t o r edu ce t h e dos age of m i l k by 15- 20% for 3- 4 meals and give to the animal the s ame quantity of liquid. CAL VE S I N S T AB L E S Jus t after colos tr um 20 g/meal the fir s t week and then r educe the dos age to 10 g/meal. N .B . T he fur will become br ighter . MI L K I N G COW S 100 g/day dur ing the fir s t week, as s oon as faeces become homogeneous r educe the dos age to 50 gr . 100/150 g / meal in cas e of s er ious dys enter y, as s oon as the pr oblem is s olved, r etur n to the us ual dos ages . N .B . I f D I AR S T OP is dos ed continuous ly in the unifeed car r iage at 10- 20 g/meal it r educe by 50% the cas es of r umen movement. P I GS Dos age: fr om 0,5 % to 2% in the feed up to 35 K g, at 1% in the feed for weaning and at 0,5 % in the feed for finis hing. T U R K E YS Dos age: fr om 0,5% to 2% for the fir s t per iod feeds , at 1% dur ing the s econd per iod and at 0,5 % dur ing the final per iod. E GG L AY E R S Dos age: fr om 0,3% to 0,5% for the fir s t 3- 4 months , then incr eas e to at 1% dur ing las t months of the cycle. R AB B I T S D I AR S T OP is us ed at dos ages that may var y fr om 1% to 2,5% in accor dance with the r es ults we want to obtain.
3

GOAT S Dos age: fr om 1% to 2% in the feed for the fir s t 25 days , fr om 0,5% to 1% dur ing the final phas e. DURI NG T HE PROCES S I NG OF PEL LE T I Z ED FE ED I ncr eas es the s helf life and the efficacy of the pelletiz ing machine. Eliminates the r es idues of powder of the pellet. I ncr eas es the har dnes s of the pellet.

PI GS T OP
L is t of tes ts Compar is on with tes ter s . Compar is on among 4 kinds of integr ator s . Feed mix tur es differ ently adde

T ES T N.01 T it l e :
A tr ial was car r ied out on pigs in or der to s tudy the effects of P I GS T OP , when added to the feed mix tur e. Mater ials and methods : I n the tr ial 120 pigs wer e divided into 2 gr oups of 60 s ubj ects each unifor m in s ex , age, weight and nes t. A gr oup, the contr ol, was given a commer cial feed mix tur e, wher eas the other gr oup r eceived the s ame feed mix tur e with the addition of 3 0 0 gr . P I GS T OP /q feed mix tur e.T he tr ial s tar ted at weaning (about 29 days of age ) and ended after 33 days when the pigs wer e about 59- 64 days old. R es u l t s : Nr . Animals Days On T r ial I nitial Weight Final Weight Daily Gain Feed Conver s ion Efficiency (K g Feed/K g Gain) Daily Feed I ntake Mor tality gr gg kg kg 3 0 0 gr . P i gs t op X 1 0 0 K g f eed 60 33 6.01 16.70 325 1.89 615 52% 5% CONT ROL 60 33 6.04 16.58 310 2.02 646 48% 5%
5

T E S T N.02 T it le : T r ials wer e car r ied out on 4 gr oups of pigs fed with a feed mix tur e having 4 differ ent additions . Mater ials and methods : I n the tr ial 80 pigs wer e utiliz ed, they wer e divided into 4 gr oups unifor m in s ex , age and weight. T he gr oups r eceived the s ame feed mix tur e but containing 4 differ ent additions . (tab.1). T he tr ial s tar ted at the wear ing when the pigs wer e about 28 days old and ended after 41 days when the s ubj ect wer e about 70 days old. R es u l t s : T AB L E 1 : Addition of the 4 ex amined feed mix tur es F eed m i x t u r e w i t h m i n er al vi t am i n i c addi t i on Feed mix tur e with miner al vitaminic addition + 300 gr . of P I GS T OP Feed mix tur e with miner al vitaminic addition + 80 gr . of CARB ADOX 10x 100K g. + 40 ppm of AVOPARCI NA Feed mix tur e with miner al vitaminic addition + 80 gr . of di CARB ADOX 10 x 100 K g. + a pr oduct containing yeas t and lactobacilli.

T AB L E 2 Feed Mix tur e Nr . Animals Days On T r ial I nitial Weight Final Weight Daily Gain (Gr ) Feed Conver s ion Efficiency Daily Feed I ntake Feed Mix tur e Efficiency Mor tality T E S T N. 03 T itle : T r ial r es ults car r ied out on pigs fed with feed mix tur e differ ently added. Mater ials and methods : I n the tr ial 140 pigs wer e utiliz ed and they wer e divided into 2 gr oups unifor m in s ex , age and weight. T he gr oups on tr ial r eceived feed mix tur e with 2 differ ent additives . T he tr ial s tar ted at the 65 th days of age and las ted 35 days . 1 20 41 5.84 19.01 321 2.04 655 49% 5% 2 20 41 5.77 20.62 374 1.88 680 53.2% 0% 3 20 41 5.86 20.31 352 1.97 695 50.7% 0% 4 20 41 5.69 20.06 350 2.00 700 50% 5%

R es u l t s : T AB L E N.01 - Addition to the 2 feed mix tur es . A feed mix tur e with miner al vitaminic addition + T YLAD 40 FURANI CO 100 CARB ADOX 10 B Feed mix tur e with miner al vitaminic addition + P I GS T OP T YLAD 40 CARB ADOX 10 T AB L E N. 02 Feed Mix tur e Nr . Animals Days On T r ial I nitial Weight Final Weight Daily Gain Feed Conver s ion Efficiency Feed Mix tur e Efficiency gg kg kg gr 1 70 35 18.90 41.30 640 2.61 38.21 2 70 35 18.81 43.15 695 2.40 1.60% 300 gr x 100Feed 50 gr x 100Feed 50 gr x 100Feed 100 gr x 100Feed 100 gr x 100Feed 80 gr x 100Feed

T S T OP
T itle : T he us e of T S T OP in the feeding of tur keys . Mat er i als an d m et h ods : 5
T he tr ial las ted 50 days when the s ubj ect wer e about 100 days old. I n the tr ial 120 animals wer e utiliz ed, they wer e divided into 2 gr oups of 60 s ubj ect each. T he tur keys wer e weighed at the beginning of the tr ial and at the end at about 150 days of age. B oth the tur keys of gr oup 1 (ex per imental thes is ) and the tur keys of gr oup 2 (contr ol) wer e given two kinds of feed mix tur e. T he only differ ence between the 2 gr oups was the s upplementar y addition of 300 g/100 K g of T S T OP . I n table 1 ther e ar e the chemical analys es of the 2 feed mix tur es given, type A fr om 100 th to 125 th day of age and type B fr om the 120 th day to the s laughter day.

T AB . NR.1 T YPE A Mois tur e Cr ude Pr otein Ether Ex tr act Cr ude Fibr e As h Cys tine Methionine Lys ine 13.00 21.00 7.50 3.00 6.30 0.20 0.35 0.80 T YPE B 13.00 18.50 9.50 3.00 6.00 0.20 0.30 0.60

I n table 2 ther e ar e the additions per K g to the feed mix tur e (ty- pe A and type B )
9

T AB . NR.2 T YPE A Vitam. A Vitam. D3 Vitam. E Vitam. B 1 Vitam. B 2 Vitam. B 6 Vitam. B 12 Vitam. K 3 Vitam. Pp Vitam. H Cholin D. Pantothenic Ac Folic Acid Cobalt I r on I odine Manganes e Copper Z inc S elenium T YPE B 14.000 U.I Vitam. A 4.500 U.I . Vitam. D3 30 mg Vitam. E 4 mg Vitam. B 1 6 mg Vitam. B 2 5 mg Vitam. B 6 0.02 mg Vitam. B 12 5 mg Vitam. K 3 75 mg Vitam. Pp 0.2 mg Vitam. H 750 mg Cholin 15 mg D. Pantothenic Ac. 1 mg Folic Acid 0.5 mg Cobalt 50 mg I r on 1 mg I odine 50 mg Manganes e 5 mg Copper 100 mg Z inc 0.050 mg S elenium 10.000 U.I . 2.500 U.I . 20 mg 2 mg 4 mg 3 mg 0.02 mg 3 mg 50 mg 0.2 mg 750 mg 15 mg 1 mg 0.5 mg 50 mg 1 mg 50 mg 5 mg 100 mg 0.050 mg
10

Res ults : T AB LE N.03 3 0 0 gr T S T OP x 1 0 0 K g F E E D CONT ROL Nr . Animals Days On T r ial I nitial Weight Final Weight T otal Gain Daily Gain Feed Conver s ion Efficiency Feed I ntake Yield Mor tality Mois tur e Litter 3.24 22.19 30.8% 6.6% 30% 3.46 22.28 28.9% 8% 35% gr gg kg kg 60 50 8.05 14.90 6.85 137 60 50 8.16 14.60 6.44 129

11

T DAI RY
T E S T 01 T I T LE DE S CRI PT I ON RES ULT S T AB L E CONCL US I ON T E S T NR. 01 T itle: Repor t and r es ults fr om the us e of T D AI R Y in the daily feed por tion to milking cows in a dair y far m in S or es ina (Cr emona) Des cr iption of the tes t: I n the far m ar e br ed animals of the s ame s pecies , on the aver age weight, age and or igin ar e the s ame. We car r ied out a tes t adding T D AI R Y in the following per iods and at the following dos ages :

PE RI OD 01/02/02 28/02/02 11/03/02 18/03/02 18/03/02 -

QUANT I T Y 25 gr / cow / day 50 gr / cow / day 25 gr / cow / day

12

Res ults : I n the following table ar e the analys es done by the Z oopr ophylactic I ns titute on the milk pr oduced by the cows dur ing the tr eatment: D at e 07/02/2002 18/02/2002 11/03/2002 18/03/2002 03/04/2002 16/04/2002 S omatic cells /ml 422.000 367.000 545.000 296.000 332.000 263.000 Geom. aver age bacter ial count 89.000 76.000 81.000 61.000 61.000 52.000 Geom. aver age cells 422.000 437.000 453.000 405.000 383.000 358.000

As you can s ee fr om the table the analys es done on Mar ch, 11 2002 the s omatic cells of the milk have incr eas ed. T his was due to the fact that cut maiz e had finis hed in one container and it was poor quality fodder . We incr eas ed the quantity of T D AI R Y and gave to the animals 50 gr /animal. As you can s ee fr om the s econd analys is of the month the par ameter s have then r etur ned back to the us ual amount. T he s light incr eas e of s omatic cells on Apr il, 4 was due to the opening of a new container of cut maize, and again the fodder was not of good quality. Fr om that date on the dos age was r educed again to 25 gr /animal.

13

Mor eover the milk pr oduction incr eas ed as we can obs er ve in the following table.

M IL K P R O D U C T IO N
QUANTITY OF MILK (100 60 40 lt) 20 0 M O R N IN G E V E N IN G 0 7 /0 2 / 1 8 /0 2 / 1 1 /0 3 / 1 8 /0 3 / 0 3 /0 4 / 1 6 /0 4 / 4 8 ,2 8 4 2 ,3 4 4 9 ,8 2 4 5 ,2 4 5 0 ,4 2 4 9 ,6 5 1 ,6 8 4 5 ,0 8 5 2 ,5 5 4 5 ,0 8 5 0 ,5 4 4 ,7 6 M O R N IN G E V E N IN G

DATE

Con cl u s i on :

T he us e of T D AI R Y at the above dos ages gr eatly r educed s omatic cells in the milk. I n gener al us ing this pr oduct ther e have been evident benefits s ince the animals can diges t the fodder better and the faeces ar e mor e cons is tent.
14

RAB S T OP
L I S T OF T ES T S T r ial car r ied out in or der to s tudy the effects of R AB S T OP , when added to the feed mix tur e, on r abbits . T he us e of a cer eals additive R abs t op in the fattening of r abbits T E S T N. 01 T itle : T r ial car r ied out in or der to s tudy the effects of R AB S T OP , when added to the feed mix tur e, on r abbits . Mater ials and methods : I n the tr ials 136 r abbits wer e utiliz ed, they wer e divided into two gr oups of 68 s ubj ects each unifor m in s ex , age weight and nes t . A gr oup, the contr ol, was given a feed mix tur e without any additives , wher eas the other gr oup r eceived the s ame mix tur e wher e R AB S T OP was added (300 gr R AB S T OP /kg feed). T he tr ial s tar ted at the weaning (about 28 days of age) and ended after 43 days when the s ubj ect wer e s laughter ed.

15

R es u l t s :
R AB S T OP 3 0 0 gr / 1 0 0 K g. f eed Nr . animals Days on tr ial I nitial weight Final weight Daily gain Feed conver s ion efficiency (K g feed/Kg gain) Daily feed intake Mor tality gr gg kg kg gr 68 43 665 2.294 37,8 3,09 117 32% 4,41% CONT ROL 68 43 667 2.218 36,1 3,38 122 29,5% 5,88%

T E S T N. 02 T it le : T he us e of a cer eals additive R AB S T OP in the fatt ening of r abbits , (this r es ear ch was car r ied out by pr of. Manfr edo Manfr edini c/o the Rationing Pr ovis ions I ns titute, Mar kets and indus tr ies of animal or igin food , Univer s ity of B ologna, Faculty of Medicine and Veter inar y S cience).

16

Mater ials and methods : I n the tr ials 144 hybr id r abbits wer e utilized, they wer e divided into 3 gr oups of 48 s ubj ects each unifor m in s ex , age, weight and nes t. A gr oup, the contr ol, was given a feed mix tur e, wher eas the other two gr oups , A1 and A2, r eceived the s ame mix tur e as the contr ol but with the addition of 250 and 300 g R AB S T OP / 100 K g. feed r es pectively. T he tr ial, which las ted 68 days , s tar ted at the weaning of animals (about 80 days of age) and ended at the s laughter . T he r abbits wer e weighed individually at inter vals of two weeks , then they wer e weighed for the las t time on the day of the s laughter . At the s ame inter vals feed intakes and r es pective feed conver s ion indices wer e r ecor ded. At the s laughter (at a weight of appr ox imately 2.5 Kg. after fas ting for 8 hour s ) live weight and weight of the chilled car cas s wer e meas ur ed. Refr iger ation took place in a r efr iger ation cell at a temper atur e of 4 C for 2 hour s . R es u l t s : T he feed intakes did not differ s tatis tically in the thr ee gr oups ; however the feed intakes tended to r educe with the addition of R AB S T OP ; the s malles t inges tion s eemed to be influenced by the level of addition (106,9 VS 108,9 VS 111,5 g/d r es pectively in A2, A1, and C). T his tendency is mor e clear in table 4 wher e the feed intakes of r abbits fed with added feed mix tur e tended to r educe fr om the fir s t days of the pos t- wear ing. T he feed conver s ion indice decr eas ed on account of the weight gains which ar e s imilar in the 2 gr oups . I n table 4 the tendency of the feed conver s ion in the differ ent phas es is als o s howed.

17

Con cl u s i on s : T he r es ults obtained s howed that the us e of R AB S T OP pos itively affected the per for mances of fattening r abbits and the feed intakes and conver s ions . Par ticular y, the r abbits fed with R AB S T OP (in the dos age 100 gr /100 kg feed mis tur e) s howed a tendency to r educe feed intakes and to limit the feed conver s ion indice. T he mor tality r ate was als o r educed in the gr oups r eceiving the addition . All thes e r es ults s howed that the pr oduct on tr ial pos itively affected the per for mances of fattening r abbits . GR OU P S P AR AME T E R S Mois tur e Cr ude Pr otein Ether Ex tr act Cr ude Fibr e As h Nitr ogen Fr ee Ex tr act Calcium Phos phor us % % S .S . CON T R OL 5,40 17,68 3,08 18,66 8,82 51,76 1,12 0,37
(1) (1)

A1 5,30 17,50 3,22 18,57 8,95 51,76 1,15 0,37

A 2 5,30 17,37 3,16 18,74 9,02 51,71 1,10 0,37

(1)

R AB S T OP has got this chemical compos ition : Cr ude pr otein 10,67 ; Ether ex tr act 3,24 ; Cr ude fibr e 9,46% ; As h 3,09% ; Nitr ogen- fr ee ex tr act 73,54 ; NDF 18,08 ; ADF 14,64 ; Calcium 0,03 ; Phos phor us 0,05 ; pH 5,60.
18

T AB . 2 - P R OD U CT I VE P E R F OR MAN CE S P AR AME T E R S Nr . Animals I nitial Weight Final Weight Daily Gain Daily Feed I ntake Feed Conver s ion Efficiency P E R I OD 0 : 14 D 15 : 28 D 29 : 42 D 43 : 56 D 57 : 68 D 0 : 68 D ** A,B a,b : : : P < 0,01 P < 0,01 P < 0,05 Kg Kg g g Con t r ol 43 0,65 2,52 27,5 111,5 4,06 A1 45 0,65 2,48 26,9 108,9 4,05 A2 47 0,65 2,49 27,1 106,9 3,94 Var i an ce 0,01 0,03 5,52 5,28 0,004 S i gn i f i can cy N.S . N.S . N.S . N.S . N.S .

T AB . 3 - W E I GH T GAI N S I N T H E D I F F E R E N T P E R I OD S ( gr / d) Con t r ol 29,5 31,0 30,6 Aa23,1 24,8 26,9 A1 29,3 30,5 30,4 B b19,1 27,5 26,9 A2 29,0 29,3 31,8 Aba21,5 25,3 27,1 Var i an ce 22,2 30,3 16,7 22,5 57,3 5,52 S i gn i f i can cy N.S . N.S . N.S . ** N.S . N.S .

19

T AB . 4 - F E E D I N T AK E AN D F E E D E F F I CI E N CY I N T H E D I F F E R E N T P E R I OD

P E R I OD 0 : 14 D 15 : 28 D 29 : 42 D 43 : 56 D 57 : 68 D 0 : 68 D FEED E FFI CI E NCY 0 : 14 D 15 : 28 D 29 : 42 D 43 : 56 D 57 : 68 D 0 : 68 D * : 0,05 a,b : 0,05 P P < <

Con t r ol 73,5 85,9 124,8 166,7 111,5 2,49 2,74 4,13 5,41 6,78 4,06

A1 71,6 84,6 121,4 167,3 108,9 2,25 2,78 3,94 6,37 6,13 4,05

A2 67,6 84,3 124,4 157,5 106,9 2,33 2,90 3,77 5,83 6,22 3,94

Var i an ce 12,8 35,6 4,60 7,70 36,6 5,28 0,04 0,03 0,04 0,08 0,13 0,04

S i gn i f i can cy N.S . N.S . N.S . N.S . N.S . N.S . N.S . N.S . N.S . N.S . N.S . N.S .

126,1 a 120,5b 119,0 b

20

T AB . 5 - S L AU GH T E R D AT A P AR AME T E R S . Nr . Animals WEI GHT At S L AUGHT ER Weight Los s T r ans por t Car cas s Chilling Weight T he K g Dur ing % After K g % Con t r ol A 1 43 2,411 4,35 1,473 61,10 45 2,368 4,43 1,425 60,11 A2 47 2,383 4,33 1,433 60,16 Var i an ce 0,03 1,57 0,02 6,18 S ignificancy N.S . N.S . N.S . N.S .

Dr es s ing Per centage %

T AB . 6 - RAB B I T S DI ED DURI NG T HE COURS E OF T HE T RI AL CAGE S PERI OD 0- 14 15- 28 29- 42 43- 56 57- 68 CON T R OL 1 0 0 2 0 0 2 0 0 1 0 1 3 0 0 1 0 0 A1 1 0 0 1 0 0 2 0 0 0 0 1 3 0 0 0 1 0 A2 1 0 0 0 0 0 2 0 0 1 0 0 3 0 0 0 0 0 0 0 6 1 2
21

T OT AL P E R I OD

CALFS T OP
T EST S: 1 Compar ing tr ial between two gr oups of white meat calves . 2 Contr ol of the affection of Cal f s t op on the main pr oductive per for mances of white meat calves . T E S T N. 0 1 Compar ing tr ial between two gr oups of white meat calves . Mater ials and methods : T he br eeding of 150 heads was made up of s ubj ects of the s ame br eed, quite unifor m in weight, age and or igin. T he tr ial was car r ied out on 70 calves which wer e given CAL F S T OP as follows : DAYS ON T RI AL 2
nd st

QUANT I T Y 5 gr / head / meal 8 gr / head / meal

T OT AL QUANT I T Y 300 gr 512 gr 812 gr

30

th rd

days days

121

- 153

T OT AL

T he other 80 calves (the contr ol) r eceived the s ame feed mix tur e (kind and quantity of dr ied milk, additions ther apy etc.) without any CAL F S T OP .
22

T he tr ial s tar ted when the young calves ar r ived and ended when the calves wer e s laughter ed. Res ults : T AB L E 1 CAL F S T OP AD D E D T O MI L K nr . Animals I nitial weight Final weight Feed conver s ion efficiency T otal feed intake (Kg) Milk yield Mor tality T E S T N. 0 2 Contr ol of the affection of CAL F S T OP on the main pr oductive per for mances of white meat calves . Mat er i als an d m et h ods : I n the tr ial 220 calves wer e utilized. T he calves , of the s ame br eed, quite unifor m in weight, age and or igin wer e divided into 2 gr oups of 116 and 104 s ubj ects r es pectively. 70 54.6 240.44 1.60 298 62.3% 2.8% CONT ROL 80 53.8 235.1 1.64 298 60.8% 2.5%

23

T he tr ial was car r ied out on 116 calves of a gr oup which wer e given CAL F S T OP as follows : DAYS ON T RI AL 2 26 127
nd th th

QUANT I T Y 6 g/head / meal 3 g/head / meal 8 g/head / meal

T OT AL QUANT I T Y 276 g 600 g 368 g 1.244 g

- 25 - 150

th th th

days days days

- 126

T OT AL

T he B gr oup (the contr ol) made up of 104 s ubj ects r eceived the s ame feed mix tur e (in quantity of dr ied milk, addition, ther apy ) as the A gr oup did but without any CAL F S T OP addition. T he tr ial s tar ted when the young calves ar r ived and ended when the calves wer e s laughter ed. Res ults : CAL F S T OP Nr . animals I nitial weight Final weight Feed conver s ion efficiency T otal feed intake (Kg) Milk yield Mor tality AD D E D T O MI L K 116 60,3 238,9 1,61 287,52 61,9% 0,8% Contr ol 104 61,6 234,5 1,67 288,80 59,8% 1,9%
24

LAYE RS T OP
T it l e :
Ex per imental tr ial on layer s dur ing laying in or der to s tudy mix tur e ( 300gr .LAYERS x 100K g feed). Mater ials and methods : T he feed was always given ad libitum. Dur ing the fir s t week feed mix tur e for pullet was given, then it was gr adually r eplaced by a feed mix tur e for layer . T he tr ial las ted about 37 weeks as data fr om 26 th to 62 nd week of age wer e r ecor ded. the effects of L AY E R S T OP when added to the feed

25

T AB . NR. 1 CH E MI CAL AN AL Y S I S Mois tur e Cr ude Pr otein Ether Ex tr act Cr ude Fibr e As h Cys tine Methionine L ys ine 13.00% 17.00% 4.50% 4.40% 13.00% 0.25% 0.30% 0.60% I N T E GR AZ I ON E P E R K G Vit. A Vit. D3 Vit. E Vit. K 3 Vit. B 1 Vit. B 2 Vit. B 6 Vit. B 12 Vit. Pp Pantothenic Acid Choline Manganes e I r on Z inc Copper Cobalt I odine Folic Acid 12.500 2.500 20 2 3 5 3 30 12 500 90 40 60 6 U.I . U.I . mg mg mg mg mg mg mg mg mg mg mg mg

0.02 mg

0.25 mg 1.2 mg 0.5 mg

26

T AB . NR. 2 L AY E R S T OP AD D E D Age Week s 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 % layin g 29.9 55.9 71.7 81.7 84.4 84.8 84.2 84.3 87.5 87.8 87.8 87.4 86.7 84.7 85.7 85.7 84.6 84.3 3.5 3.4 3.4 3.0 3.1 3.0 3.0 3.2 3.4 3.4 3.3 3.0 3.0 3.1 3.4 % br ok eggs egg layer s feed intake weigh left t 50.8 52.1 56.0 57.8 58.1 58.6 59.4 60.5 61.2 61.6 61.8 62.1 62.8 62.8 62.9 63.0 62.8 63.1 27.330 27.265 27.180 27.143 27.096 27.041 26.989 26.934 26.886 26.854 26.823 26.775 26.730 26.670 26.626 26.581 26.541 26.492 112 110 112 110 W I T H OU T L AY E R S T OP % laying 29.8 55.3 71.4 81.5 84.1 84.6 84.3 84.1 86.6 86.7 86.4 86.1 85.3 85.0 85.0 84.8 84.2 84.1 3.5 3.8 4.1 4.0 4.1 3.9 3.8 3.7 3.8 3.8 3.9 4.0 4.1 4.3 4.6 % br ok eggs 51.2 52.3 54.5 56.4 57.1 57.6 57.8 58.1 59.3 60.1 60.2 60.5 60.8 61.3 61.4 60.9 61.5 61.8 27.315 27.245 27.210 27.173 27.151 27.112 27.083 27.021 26.963 26.931 26.893 26.815 26.781 26.703 26.641 26.603 26.554 26.512
27

Egg Weight

L ayer s left

Feed I ntake

114

120

116

118

40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62

83.4 80.0 78.8 79.5 80.2 79.4 78.9 75.5 75.3 73.7 74.0 74.0 74.1 74.0 73.5 72.2 72.6 73.6 74.2 73.1 70.2 72.8 70.3

4.0 4.0 4.0 4.1 4.2 4.3 4.6 4.2 4.4 4.2 4.1 4.3 4.4 4.1 4.0 4.2 4.6 4.6 4.5 4.2 4.4 4.6 4.3

63.3 63.3 63.5 63.3 64.1 64.0 64.2 63.9 64.3 64.5 64.6 64.6 64.8 65.1 65.0 64.8 64.9 65.4 66.0 65.9 66.1 66.4 66.3

26.445 26.387 26.330 26.295 26.260 26.227 26.193 26.125 26.101 26.041 25.994 25.942 25.790 25.773 25.760 25.741 25.726 25.690 25.653 25.621 25.593 25.541 25.496 102 104 107 106 106 108

83.6 81.6 80.5 80.1 80.1 80.3 79.6 73.6 78.8 74.6 74.2 74.1 73.0 72.6 73.1 70.8 69.3 68.8 68.6 68.8 68.1 67.9 66.2

4.9 5.0 5.0 5.2 4.9 5.1 5.2 5.3 5.6 5.7 5.7 5.8 6.0 5.7 6.1 5.9 6.1 6.0 6.3 6.1 6.2 6.8 7.4

62.1 62.0 62.2 62.4 62.8 62.6 62.9 63.3 63.2 63.4 63.4 63.8 63.6 63.5 63.9 64.0 64.1 64.3 64.5 64.7 64.6 64.9 65.0

26.451 26.383 26.312 26.261 26.208 26.143 26.093 26.002 25.937 25.841 25.717 25.666 25.593 25.521 25.488 25.416 25.368 25.294 25.231 25.118 25.073 25.017 24.983 111 113 114 116 115 116

28

R es u l t s : T AB L E N .0 3 CAP .1 L aying B r oken Eggs Egg Weight Mor tality Feed I ntake Con cl u s i on s : T he r es ults obtained (s ee table 3) s howed that the addition of L AYERS T OP ( 300 gr x 100Kg feed) to the feed mix tur e pos itively affected the per for mance of the layer s .We could s ee how the laying par centage (79.3% VS 78.4% ) the egg weight (63.5 VS 62.1) was better among the layer s in the s hed num- ber 1. T he br oken eggs per centage (3.86 VS 5.04) and the daily feed intake ( 107.7 gr VS 115.3 gr ) s howed how the addition pos itively affected the per for mance of the animals . We could infer that the s ugges ted addition could r educe the pr oduction cos t/K g eggs of ar ound 7- 8% . % % 79.3 3.86 63.5 6.7% 107.7 CAP .2 78.4 5.04 62.2 8.53% 115.3

29

ENZ YMES FOR WHEAT B AS ED FEEDS B ROI LER PERFORMANCE T RI AL T S T OP

A tr ial was conducted with an integr ated br oiler company to inves tigate the effects of us ing in br oiler feeds . T r i al s i z e: T r i al f or m at : 75.000 bir ds fr om day old 6 hous es . 3 hous es (37.500 bir ds ) fed s tandar d diet (contr ol), 3 hous es (37.500 bir ds ) fed tr ial diet with T B R OI L E R S at the dos age of 3 K g/T on (tr eatment)

30

 Feed:

The two groups have been fed with the same quantity (3 Kg animal)
S L AU GH T E R L I V E W E I GH T ( K g) S L AU GH T E R AGE ( d ay s ) D AI L Y W H E I GH T GAI N ( g m / d ay ) F CR ( k g)

CONTROL GROUP HOUSE 1 HOUSE 2 HOUSE 3 AVERAGE TREATMENT GROUP HOUSE 4 HOUSE 5 HOUSE 6 AVERAGE IM P R O V . O V E R C O N T R O L % IM P R O V E M E N T

1 .7 6 5 1 .6 9 0 1 .7 8 0 1 .7 4 5 ,0 0

42 42 41 4 1 ,6 7

4 2 ,0 2 4 0 ,2 4 4 3 ,4 1 4 1 ,8 9

1 ,7 0 1 ,7 8 1 ,6 9 1 ,7 2

1 .8 0 5 1 .8 5 1 1 .8 0 7 1 .8 2 1 ,0 0 7 6 ,0 0 4 ,3 6

41 41 40 4 0 ,6 7 -1 ,0 0 2 ,4 6

4 4 ,0 2 4 5 ,1 5 4 5 ,1 8 4 4 ,7 8 2 ,8 9 6 ,9 0

1 ,6 6 1 ,6 2 1 ,6 6 1 ,6 5 0 ,0 7 4 ,3 6

OB S E R VAT I ON T B R OI L E R S tr eated feed r es ulted in a 6,90% impr ovement in Daily Liveweight Gain and in a 4,36% impr ovement in Feed Conver s ion Ratio. B ir ds fed T B R OI L E R S tr eated feed finis hed mor e quickly (one day befor e), at heavier weights
31

D ai l y L i vew ei gh t Gai n

45 44 Control 43 TBROILERS 42 41 40

32

F eed Con ver s i on R at i o

2 Control TBROILERS

1
33