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Name: _________________________________________

Class: ________

CHAPTER 1 (MICROORGANISM)
1. Effect of pH factor on the growth of microorganisms
Aim : ____________________________________________________________________________________________.
Problem statement : What is the effect of pH value on the growth of bacteria?
Hypothesis : ______________________________________________________________________________________
Variable:

Manipulated : _____________________________________________.
Responding

: _____________________________________________.

Constant

: _____________________________________________.

Materials and Apparatus:

1.

_____________________

5.

_______________________

2.

_____________________

6.

_______________________

3.

_____________________

7.

______________________

4.

_____________________

sterile
cotton

nutrient broth +
Bacteria culture
A
acidic

Sterile
cotton

nutrient broth +
Bacteria culture
B
pH 7 (neutral)

nutrient broth +
Bacteria culture
C
Alkaline

Procedure:
1.
2.

Three test tubes with nutrient broth are labelled A, B and C.


The solutions below are added to the test tubes:
A: 1 ml of dilute hydrochloric acid + 1 ml of bacterial culture
B: 1 ml of distilled water + 1 ml of bacterial culture
C: 1 ml of sodium hydroxide solution + 1 ml of bacterial culture

3.
4.
5.

The test tubes are covered with a steril cotton.


All the three test tubes are kept in a cupboard.
After 4 days, observation on the growth of bacteria is recorded.

Tabulation of data:

Conclusion:
___________________________________________________________________________________

Prepared by Pn. Sity Salang

Name: _________________________________________

Class: ________

2. Effect of temperature factor on the growth of microorganisms:


Aim :__________________________________________________________________________________________.
Problem statement : What is the effect of temperature on the growth of bacteria?
Hypothesis : ___________________________________________________________________________________.
Variable:
Manipulated:

_____________________________________________

Responds

_____________________________________________

Constant

_____________________________________________

Materials and Apparatus:

1.

_____________________

5.

_______________________

2.

_____________________

6.

_______________________

3.

_____________________

7.

______________________

4.

_____________________

Sterile cotton

nutrient broth +
Bacteria culture

nutrient broth +
Bacteria culture
A (50C )

B (370C )

nutrient broth +
Bacteria culture
C (600C )

Procedure:
1. Three test tubes with nutrient broth are labelled A, B and C.
2. 1 ml of bacterial culture is poured onto the nutrient broth in each test tube.
3. The test tubes are covered with a steril cotton.
4. The test tube are placed in various places as stated below:
A: kept in a refrigerator at temperature 50C
B: kept in an oven at temperature 370C
C: kept in an oven at temperature 800C
5. After 4 days, observation on the growth of bacteria is recorded.

Tabulation of data:

Conclusion:
_____________________________________________________________________________.
Prepared by Pn. Sity Salang

Name: _________________________________________

Class: ________

3. Effect of light on the growth of microorganisms:


Aim : __________________________________________________________________________.
Problem statement : What is the effect of light on the growth of bacteria?
Hypothesis : ____________________________________________________________________.
Variable:
Manipulated:

____________________________

Responds

____________________________

Constant

____________________________

Materials and Apparatus:

1.

____________________

4.

____________________

2.

____________________

5.

___________________

3.

____________________

sterile cotton

nutrient broth +
Bacteria culture
A (dark)

nutrient broth +
Bacteria culture
B (under bright light)

Procedure:
1. Two test tubes with nutrient broth are labelled A and B.
2. 1 ml of bacterial culture is poured onto the nutrient broth in each test tube.
3. The test tubes are covered with a steril cotton.
4. The test tubes are placed in various places as stated below:
A: is placed in a dark condition
B: is left under bright light.
5. After 4 days, observation on the growth of bacteria is recorded.
Tabulation of data:

Conclusion:
____________________________________________________________________________.

Prepared by Pn. Sity Salang

Name: _________________________________________

Class: ________

4. Effect of humidity on the growth of microorganisms:


Aim : ____________________________________________________________________________.
Problem statement : What is the effect of humidity on the growth of bacteria?
Hypothesis : ______________________________________________________________________.
Variable:
Manipulated:

____________________________________

Responds

____________________________________

Constant

____________________________________

Materials and Apparatus:

1.

_____________________

4.

________________

2.

_____________________

5. _________________

3.

____________________

6. _________________

sterile cotton

Damp nutrient broth +


Bacteria culture
A (humid)

Dry nutrient broth +


Bacteria culture
B (dry)

Procedure:
1. Two test tubes are labelled A and B, and their contents are shown below.
A: Damp nutrient broth + 1 ml of bacterial culture
B: Dry nutrient broth + 1 ml of bacterial culture
2. The test tubes are covered with a steril cotton.
3. All the three test tubes are kept in a cupboard.
4. After 4 days, observation on the growth of bacteria is recorded.
Tabulation of data:

Conclusion:
_______________________________________________________________________________.

Prepared by Pn. Sity Salang

Name: _________________________________________

Class: ________

5. Effect of nutrient on the growth of microorganisms:


Aim : ____________________________________________________________________________
Problem statement : What is the effect of nutrient on the growth of bacteria?
Hypothesis : ______________________________________________________________________
Variable:
Manipulated:

______________________

Responds

______________________

Constant

_______________________

Materials and Apparatus:

1.

____________________

5.

___________________

2.

____________________

4.

___________________

3.

____________________

sterile cotton

Nutrient broth +
Bacteria culture

A
(presence of nutrient)

Broth without nutrient +


Bacteria culture

B
(without nutrient)

Procedure:
1. Two test tubes are labelled A and B, and their contents are shown below.
A: damp nutrient broth + 1 ml of bacterial culture
B: damp broth without nutrient + 1 ml of bacterial culture
2. The test tubes are covered with a steril cotton.
3. All the three test tubes are kept in a cupboard.
4. After 4 days, observation on the growth of bacteria is recorded.
Tabulation of data:

Conclusion:
____________________________________________________________________________.

Prepared by Pn. Sity Salang

Name: _________________________________________

Class: ________

6. Effect of antibiotic on the growth of microorganisms:


Aim :____________________________________________________________________________.
Problem statement : What is the effect of antibiotic on the growth of bacteria?
Hypothesis : _____________________________________________________________________.
Variable:
Manipulated:

_________________________________

Responds

_________________________________

Constant

__________________________________

Materials and Apparatus:

distil water
disc

1.

_______________________

5.

______________________

2.

______________________

6.

______________________

3.

______________________

7.

_____________________

4.

______________________

nutrient agar
+
bacteria culture

penicillin disc

Procedure:
1. Two Petri dishes with nutrient agar are labelled A and B.
2. 1 ml of bacterial culture is poured onto the nutrient agar in each Petri dish.
3. A dish of filter paper that has been moistened with distil water is placed on the surface of
nutrient agar in Petri dish A as shown above.
4. A penicillin disc is placed on the surface of nutrient agar in Petri dish B.
5. All the two petri dishes are kept in a cupboard.
6. After 4 days, observation on the growth of bacteria is recorded.
Tabulation of data:

Conclusion:
______________________________________________________________________________.

Prepared by Pn. Sity Salang

Name: _________________________________________

Class: ________

CAPTER 5 (MOTION)
A) INERTIA
Aim: ___________________________________________________________________________________.
Problem statement: What is the relationship between mass and inertia?
Hypothesis: _____________________________________________________________________________.
Variable:
Manipulated : _____________________________________________.
Responding

: _____________________________________________.

Constant

: _____________________________________________.

Materials and apparatus: 1. _______________________


2. _______________________
3. _______________________

Procedure:
1. Tin B is filled up with sand while tin A is left empty.
2. Both tins are swung at the same height.
3. The tin are stopped from swinging after a while.
4. The tin that is harder to swing and that is harder to stop are observed.

Tabulation Of Data:

Conclusion:
________________________________________________________________________________.

Prepared by Pn. Sity Salang

Name: _________________________________________

Class: ________

MOMENTUM
Relationship Between Mass And Momentum
Aim: ___________________________________________________________________________.
Problem statement: What is the relationship between mass and momentum?
Hypothesis: ______________________________________________________________________.
Variable:
Manipulated variable : ____________________________.
Responding variable : ____________________________
Constant variable
Materials and apparatus:
1.
2.
3.
4.

(a)

: ____________________________
________________________
________________________
________________________
________________________

(b)

Procedure:
1. The apparatus as shown in diagram (a) is set up.
2. A trolley is pushed gently to move down the track and collides a wooden block.
3. The displacement of wooden block is measured and recorded.
4. This experiment is repeated by using a two-stacked trolley as shown in diagram (b).
Tabulation Of Data:

Conclusion:

_____________________________________________________

Prepared by Pn. Sity Salang

Name: _________________________________________

Class: ________

2. Relationship Between Velocity And Momentum


Aim: ___________________________________________________________________________.
Problem statement: What is the relationship between velocity and momentum?
Hypothesis: _____________________________________________________________________.
Variable:
Manipulated : ____________________________________________________
Responding

: ____________________________________________________

Constant

: _____________________________________________________

Materials and apparatus:


1. ________________________
2. ________________________
3. ________________________
4. ________________________

(a)

(b)

Procedure:
1. The apparatus as shown in diagram (a) is set up.
2. A trolley is pushed gently down the track and it collides with a wooden block.
3. The displacement of wooden block is measured and recorded
4. This experiment is repeated by using two wooden blocks to make the track steeper as shown in
diagram (b).
Tabulation Of Data:

Conclusion:
_______________________________________________________________________________.

Prepared by Pn. Sity Salang

Name: _________________________________________

Class: ________

C) PRESSURE
1. Relationship Between Pressure And Force
Aim: ___________________________________________________________________________.
Problem statement: What is the relationship between pressure and force?
Hypothesis: _____________________________________________________________________.
Variable:
Manipulated : ____________________________________________________
Responding

: ____________________________________________________

Constant

: _____________________________________________________

Materials and apparatus:


1. ________________________
2. ________________________
3. ________________________
4. ________________________

Procedure:
1. The apparatus are prepared as shown above.
2. A 100g weight is placed on the sponge.
3. The distance of the compressed sponge is recorded.
4. Steps 2 and 3 are repeated with various loads of 200g, 300g, 400g and 500g.
5. The observation is recorded.
Tabulation Of Data:

Conlusion:
___________________________________________________________________
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Name: _________________________________________

Class: ________

2. Relationship Between Pressure And surface Area.


Aim: __________________________________________________________________________
Problem statement: What is the relationship between pressure and surface area?
Hypothesis: ____________________________________________________________________.
Variable:
Manipulated : ____________________________________________________
Responding

: ____________________________________________________

Constant

: _____________________________________________________

Materials and apparatus: 1.

____________________

2.

_______________________

2.

____________________

4.

_______________________

Procedure:
1.

A 500g weight on top of the plasticine as shown above. The impact on the plasticine is
recorded.

2.

The experiment is repeated by putting the weight on a sharp nail and a blunt nail
respectively, while their pointed edges press against the plasticine.

3.

The dent formed on the plasticine are compared.

4.

Result is recorded in a table.

Tabulation Of Data:

Conlusion:
__________________________________________________________________________.
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Name: _________________________________________

Class: ________

Aim: To study Archimedes principle.


Problem statement: What is the relationship between the buoyant force and the weight of the water
displaced?
Hypothesis: The buoyant force acting on the stone is equal to the weight of the water displaced by
the stone.
Variable:
Manipulated : Weight of stone
Responding

: Weight of water displaced.

Constant

: Type of water used

Materials and apparatus: 1.

Eureka can,

2.

Beaker

3.

Thread

4.

Stones of different sizes

5.

Spring balance

6.

Water

Procedure:
1.

Set up the apparatus as shown in the diagram above.

2.

Record the weight of the stone as shown in diagram (a).

3.

Measure the weight of the empty beaker and record it as W1.

4.

Immerse the stone into the water in the Eureka Vessel as shown in diagram (b).

5.

Record the weight of the stone when immersed in water.

6.

Measure the weight of the beaker that contains the displaced water and record it as W2.

7.

Calculate the weight of the displaced water. (W2 - W1 )

8.

Repeat steps 2-7 by using different sizes of stones.

9.

Record the observations in the form of the table.

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Name: _________________________________________

Class: ________

Result:
Weight of stone in air = 0.8 N (W3)
Weight of stone in water = 0.4 N (W4)
(appearance weight loss of the stone)
Buoyant force acting on the stone = 0.4 N (W3 - W4)
Weight of empty beaker = 0.6 N (W1)
Weight of beaker and water displaced = 1.0 N (W5)
Weight of water displaced = 0.4 N (W5 - W1)
From the results, W4 < W3
W3 - W4 = W5 - W1
Weight Of Stone in the water (N)
0.4
0.2

Weight Of Water Displaced (N)


0.4
0.2

Discussion:
From the experiment, it is observed that volume of water displaced is equal to the volume
of the object that is completely immersed in water.
The weight of the stone when it is completely immersed in water is less than the natural
weight of the stone in air. This is due to the upthrust or buoyant force of the water acting on the stone.
The amount of water in the beaker is the amount of water displaced by the stone. The
weight loss of the stone is equal to the weight of the water displaced.
Conlusion:
The buoyant force acting on the stone is equal to the weight of the water displaced by the
stone. Hypothesis is accepted.

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