Enzymes Revision Questions 1. An enzyme is completely denatured at 50 C. A fixed concentration of this enzyme is added to a fixed concentration of its substrate.

The time taken for completion of the reaction is measured at different temperatures. Which graph sho s the results!

2. A metabolic path ay is

What ould be the effect of adding a small amount of a non"competiti#e inhibitor of enzyme $! A %nzyme $ ould be partially denatured. B &ubstance ' ould increase in concentration. C &ubstance ( ould no longer be formed. D The initial reactant ould no longer be metabolised.

3. The enzyme lysozyme secreted from tear glands forms deposits on contact lenses. Which ingredient ould be effecti#e in a contact lens cleaner for remo#ing these deposits! A antibodies B lysosomes C p) buffers D proteases 4. The graph sho s the effect of substrate concentration on the rate of an enzyme" controlled reaction. The enzyme concentration is constant.

Which statement about the graph is correct! A *et een W and X+ the number of enzyme molecules is limiting. B *et een X and Y+ the number of enzyme molecules is limiting. C *et een X and Y+ the number of substrate molecules is limiting. D *et een X and Y+ the product concentration remains the same.

5. The cur#e ' sho s the acti#ity of an enzyme at $0oC. Cur#es A to , sho the effect of different conditions on the acti#ity of the enzyme. Which cur#e sho s the effect of increasing the temperature by -0oC and adding extra substrate!

6. What is the effect of increasing substrate concentration on the degree of inhibition of an enzyme"controlled reaction!

7. The initial rate of a reaction catalysed by an enzyme as measured at #arious substrate concentrations. Which graph sho s the effect of a lo concentration of non"competiti#e inhibitor on the reaction!

8. The graphs sho the effects of temperature and p) on enzyme acti#ity.

Which statement explains the enzyme acti#ity at the point sho n! A At .+ hydrogen bonds are formed bet een enzyme and substrate. B At /+ the kinetic energy of enzyme and substrate is highest. C At 0+ peptide bonds in the enzyme begin to break. D At &+ the substrate is completely denatured. . Which bonds are the last to break hen an enzyme is heated! A disulphide B hydrogen C hydrophobic interactions D ionic 1!. ,uring the production of apple 1uice+ enzymes are used to break do n the components of the cell alls. Which carbohydrate ill be produced by this hydrolysis! A amylose B cellulose C 2 glucose D 3 glucose

11. The rate of enzyme catalysed reactions in human cells is regulated. Which of the follo ing may be in#ol#ed in such regulation! - a change in enzyme concentration $ a change in substrate concentration 4 inhibition by the final product of the reaction A - only B 4 only C - and $ only D -+ $ and 4 12. The graph sho s the effect of an enzyme on a reaction.

Which combination identifies '+ ( and 5!

13. 6n the graph+ X represents the relationship bet een the initial rate of reaction of an enzyme and the concentration of its substrate under optimal conditions and ithout an inhibitor. Which cur#e represents the result hen the same experiment is carried out in the presence of a fixed+ lo concentration of a non"competiti#e inhibitor!

14. Which bonds hold substrate molecules to the acti#e site of an enzyme! A disulphide B glycosidic C hydrogen D peptide

15. 7ollo ing a heart attack+ the enzyme lactate dehydrogenase leaks into the blood plasma from damaged heart muscle. Which steps are re8uired to obtain the best estimate of lactate dehydrogenase acti#ity in a sample of blood plasma!