BOT2201CP LAB FOR PLANT ANATOMY & CELL BIOLOGY

(Lab) LEARNING OUTCOME : On successful completion of the course, the student will be able to understand the structure and function of living and non living cell inclusions in plants appreciate the architecture of plant tissues. understand tissue organization in plant parts. identify the stages of mitotic and meiotic cell divisions relate the anatomical modifications to the habitat COURSE OUTLINE : EXPERIMENTS/LAB : 1. Maceration and observation of cell types. 2. Dermal tissue mounting of epidermal peeling and observation of Trichomes and stomatal types 3. Primary and secondary structure of dicot and monocot plant parts A. Leaf Hibiscus & Grass B. Stem- Tridax & Grass, C. Root Bean & Grass 4.Nodal anatomy- Trilacunar node (Azadirachta) 5. Secondary structure of dicot stem (Sunflower) and dicot root (Bean) 6. Microtome sectioning-Demonstration (Boerhavia stem / Ficus leaf) 7. Anamolous secondary growth in Boerhavia / Dracaena / Bougainvillea / Bauhinia 8. Anatomical adaptations of Hydrophytes (Hydrilla stem) Xerophytes (Nerium leaf) 9. Observation of ergastic substances Starch grains - potato, rice Cystolith Ficus leaf Raphides - Colacacia 10. Mitosis Onion root tip squash 11. Meiosis Tradescantia anther smear 45 Hrs. 3 Hrs./Wk.

BOT2201CP LAB FOR PLANT ANATOMY & CELL BIOLOGY

(Lab)

Entry Behaviour Test

Answer the following:

1.Dicotyledon: The dicotyledons, also known as dicots, was a grouping formerly used for the flowering
plants whose seed typically has two embryonic leaves orcotyledons

2.Hydrophytes: a plant that grows in water or very moist ground; an aquatic plant.

3Monocotyledon:Monocotyledons also known as monocots, are one of two major groups of flowering plants (or angiosperms) that are traditionally recognized, the other being dicotyledons, or dicots. Monocot seedlings typically have one cotyledon (seed-leaf), in contrast to the two cotyledons typical of dicots.

4. Cystolith: (Gr. "cavity" and "stone") is a botanical term for the inorganic concretions, usually of calcium carbonate, formed in a cellulose matrix in special cells called lithocysts, generally in the leafof plants of certain families, e.g. Ficus elastica, the Indian rubber plant of the family Moraceae. Plants in the family Urticaceae, also known as Stinging Nettles, also form leaf cystoliths, but only during their later flowering and seed setting stages. Cannabis and other plants in the family Cannabaceae also produce leaf and flower cystoliths.

5.Mitosis:It is the process by which a cell duplicates into two daughter cells.

Course Facilitators

Asha Kannan and G.Priscilla Sweetlin